JP2018500936A - バブルプライマー - Google Patents

バブルプライマー Download PDF

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Publication number
JP2018500936A
JP2018500936A JP2017551376A JP2017551376A JP2018500936A JP 2018500936 A JP2018500936 A JP 2018500936A JP 2017551376 A JP2017551376 A JP 2017551376A JP 2017551376 A JP2017551376 A JP 2017551376A JP 2018500936 A JP2018500936 A JP 2018500936A
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JP
Japan
Prior art keywords
primer
sequence
nucleotides
amplification
complementary
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JP2017551376A
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English (en)
Japanese (ja)
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JP2018500936A5 (https=
Inventor
マキューン，ブライアン・ジェームズ
マケルガン，キャサル・ジョゼフ
Original Assignee
ディーエヌエーイー グループ ホールディングス リミテッド
ディーエヌエーイー グループ ホールディングス リミテッド
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Application filed by ディーエヌエーイー グループ ホールディングス リミテッド, ディーエヌエーイー グループ ホールディングス リミテッド filed Critical ディーエヌエーイー グループ ホールディングス リミテッド
Publication of JP2018500936A publication Critical patent/JP2018500936A/ja
Publication of JP2018500936A5 publication Critical patent/JP2018500936A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2017551376A 2014-12-22 2015-12-22 バブルプライマー Pending JP2018500936A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB201422982 2014-12-22
GB1422982.7 2014-12-22
PCT/GB2015/054125 WO2016102956A1 (en) 2014-12-22 2015-12-22 Bubble primers

Publications (2)

Publication Number Publication Date
JP2018500936A true JP2018500936A (ja) 2018-01-18
JP2018500936A5 JP2018500936A5 (https=) 2019-01-31

Family

ID=55025267

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017551376A Pending JP2018500936A (ja) 2014-12-22 2015-12-22 バブルプライマー

Country Status (5)

Country Link
US (1) US20170349926A1 (https=)
EP (1) EP3237635B1 (https=)
JP (1) JP2018500936A (https=)
CN (1) CN107208150A (https=)
WO (1) WO2016102956A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018009677A1 (en) * 2016-07-07 2018-01-11 Complete Genomics, Inc. Fast target enrichment by multiplexed relay pcr with modified bubble primers
CN107365769B (zh) * 2017-07-25 2021-05-04 深圳华大智造科技股份有限公司 一种鼓泡状引物及其组成的试剂盒和应用
CN111699269B (zh) * 2018-01-12 2025-12-05 纳特拉公司 新引物及其用途

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6692915B1 (en) * 1999-07-22 2004-02-17 Girish N. Nallur Sequencing a polynucleotide on a generic chip
JP2008531040A (ja) * 2005-03-05 2008-08-14 シージーン アイエヌシー 二重特異性オリゴヌクレオチドを使用した方法及び二重特異性オリゴヌクレオチド
JP2011172573A (ja) * 1998-12-23 2011-09-08 Preben Lexow 拡大タグを使用したシークエンシング方法
WO2014018080A1 (en) * 2012-07-24 2014-01-30 Natera, Inc. Highly multiplex pcr methods and compositions
JP2014180278A (ja) * 2013-03-21 2014-09-29 Toshiba Corp 核酸の解析方法、そこにおいて使用されるアッセイキット

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1896617B1 (en) * 2005-05-31 2013-01-02 Life Technologies Corporation Multiplex amplification of short nucleic acids
CN101484898A (zh) * 2006-07-04 2009-07-15 株式会社岛津制作所 核酸扩增用引物设计装置、引物设计程序、以及引物设计服务器装置
WO2011001496A1 (ja) * 2009-06-29 2011-01-06 株式会社 東芝 検体解析方法およびそれに用いるアッセイキット
WO2012103154A1 (en) * 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
CN103060924B (zh) * 2011-10-18 2016-04-20 深圳华大基因科技有限公司 微量核酸样本的文库制备方法及其应用
US20130184165A1 (en) * 2012-01-13 2013-07-18 Data2Bio Genotyping by next-generation sequencing
CN102952798A (zh) * 2012-11-07 2013-03-06 龙岩九健生物芯片技术研究所 一种pcr引物设计方法
CN111118125B (zh) * 2013-11-26 2023-06-30 杭州联川基因诊断技术有限公司 一种纯化pcr产物的方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011172573A (ja) * 1998-12-23 2011-09-08 Preben Lexow 拡大タグを使用したシークエンシング方法
US6692915B1 (en) * 1999-07-22 2004-02-17 Girish N. Nallur Sequencing a polynucleotide on a generic chip
JP2008531040A (ja) * 2005-03-05 2008-08-14 シージーン アイエヌシー 二重特異性オリゴヌクレオチドを使用した方法及び二重特異性オリゴヌクレオチド
WO2014018080A1 (en) * 2012-07-24 2014-01-30 Natera, Inc. Highly multiplex pcr methods and compositions
JP2014180278A (ja) * 2013-03-21 2014-09-29 Toshiba Corp 核酸の解析方法、そこにおいて使用されるアッセイキット

Also Published As

Publication number Publication date
EP3237635B1 (en) 2019-10-23
WO2016102956A1 (en) 2016-06-30
US20170349926A1 (en) 2017-12-07
EP3237635A1 (en) 2017-11-01
CN107208150A (zh) 2017-09-26

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