JP2018500936A5 - - Google Patents
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- Publication number
- JP2018500936A5 JP2018500936A5 JP2017551376A JP2017551376A JP2018500936A5 JP 2018500936 A5 JP2018500936 A5 JP 2018500936A5 JP 2017551376 A JP2017551376 A JP 2017551376A JP 2017551376 A JP2017551376 A JP 2017551376A JP 2018500936 A5 JP2018500936 A5 JP 2018500936A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
- nucleotides
- region
- starting template
- product
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims 11
- 239000002773 nucleotide Substances 0.000 claims 7
- 125000003729 nucleotide group Chemical group 0.000 claims 7
- 108091093088 Amplicon Proteins 0.000 claims 4
- 230000000295 complement effect Effects 0.000 claims 4
- 150000007523 nucleic acids Chemical class 0.000 claims 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 3
- 230000003321 amplification Effects 0.000 claims 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims 3
- 238000012163 sequencing technique Methods 0.000 claims 3
- 230000015556 catabolic process Effects 0.000 claims 2
- 238000006731 degradation reaction Methods 0.000 claims 2
- 238000009396 hybridization Methods 0.000 claims 2
- 108091033319 polynucleotide Proteins 0.000 claims 2
- 102000040430 polynucleotide Human genes 0.000 claims 2
- 239000002157 polynucleotide Substances 0.000 claims 2
- 238000006243 chemical reaction Methods 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB201422982 | 2014-12-22 | ||
| GB1422982.7 | 2014-12-22 | ||
| PCT/GB2015/054125 WO2016102956A1 (en) | 2014-12-22 | 2015-12-22 | Bubble primers |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2018500936A JP2018500936A (ja) | 2018-01-18 |
| JP2018500936A5 true JP2018500936A5 (https=) | 2019-01-31 |
Family
ID=55025267
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017551376A Pending JP2018500936A (ja) | 2014-12-22 | 2015-12-22 | バブルプライマー |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20170349926A1 (https=) |
| EP (1) | EP3237635B1 (https=) |
| JP (1) | JP2018500936A (https=) |
| CN (1) | CN107208150A (https=) |
| WO (1) | WO2016102956A1 (https=) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018009677A1 (en) * | 2016-07-07 | 2018-01-11 | Complete Genomics, Inc. | Fast target enrichment by multiplexed relay pcr with modified bubble primers |
| CN107365769B (zh) * | 2017-07-25 | 2021-05-04 | 深圳华大智造科技股份有限公司 | 一种鼓泡状引物及其组成的试剂盒和应用 |
| CN111699269B (zh) * | 2018-01-12 | 2025-12-05 | 纳特拉公司 | 新引物及其用途 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NO986133D0 (no) * | 1998-12-23 | 1998-12-23 | Preben Lexow | FremgangsmÕte for DNA-sekvensering |
| US6692915B1 (en) * | 1999-07-22 | 2004-02-17 | Girish N. Nallur | Sequencing a polynucleotide on a generic chip |
| WO2006095941A1 (en) * | 2005-03-05 | 2006-09-14 | Seegene, Inc. | Processes using dual specificity oligonucleotide and dual specificity oligonucleotide |
| EP1896617B1 (en) * | 2005-05-31 | 2013-01-02 | Life Technologies Corporation | Multiplex amplification of short nucleic acids |
| CN101484898A (zh) * | 2006-07-04 | 2009-07-15 | 株式会社岛津制作所 | 核酸扩增用引物设计装置、引物设计程序、以及引物设计服务器装置 |
| WO2011001496A1 (ja) * | 2009-06-29 | 2011-01-06 | 株式会社 東芝 | 検体解析方法およびそれに用いるアッセイキット |
| WO2012103154A1 (en) * | 2011-01-24 | 2012-08-02 | Nugen Technologies, Inc. | Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations |
| CN103060924B (zh) * | 2011-10-18 | 2016-04-20 | 深圳华大基因科技有限公司 | 微量核酸样本的文库制备方法及其应用 |
| US20130184165A1 (en) * | 2012-01-13 | 2013-07-18 | Data2Bio | Genotyping by next-generation sequencing |
| HK1211058A1 (en) * | 2012-07-24 | 2016-05-13 | 纳特拉公司 | Highly multiplex pcr methods and compositions |
| CN102952798A (zh) * | 2012-11-07 | 2013-03-06 | 龙岩九健生物芯片技术研究所 | 一种pcr引物设计方法 |
| JP2014180278A (ja) * | 2013-03-21 | 2014-09-29 | Toshiba Corp | 核酸の解析方法、そこにおいて使用されるアッセイキット |
| CN111118125B (zh) * | 2013-11-26 | 2023-06-30 | 杭州联川基因诊断技术有限公司 | 一种纯化pcr产物的方法 |
-
2014
- 2014-12-22 US US15/538,078 patent/US20170349926A1/en not_active Abandoned
-
2015
- 2015-12-22 CN CN201580074738.5A patent/CN107208150A/zh active Pending
- 2015-12-22 JP JP2017551376A patent/JP2018500936A/ja active Pending
- 2015-12-22 WO PCT/GB2015/054125 patent/WO2016102956A1/en not_active Ceased
- 2015-12-22 EP EP15816856.7A patent/EP3237635B1/en active Active
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