CN107201396A - 检测水稻半矮杆基因sd-1等位基因用的CAPS标记 - Google Patents
检测水稻半矮杆基因sd-1等位基因用的CAPS标记 Download PDFInfo
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- CN107201396A CN107201396A CN201610156855.2A CN201610156855A CN107201396A CN 107201396 A CN107201396 A CN 107201396A CN 201610156855 A CN201610156855 A CN 201610156855A CN 107201396 A CN107201396 A CN 107201396A
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
基因组DNA | 1μL |
上游引物(10μM) | 0.5μL |
下游引物(10μM) | 0.5μL |
2×Taq MasterMix | 10μL |
ddH2O | 8μL |
基因组DNA的PCR扩增产物 | 5μL |
酶切缓冲液 | 2μL |
限制性内切酶 | 0.5μL |
ddH2O | 12.5μL |
Claims (10)
Priority Applications (1)
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CN201610156855.2A CN107201396B (zh) | 2016-03-18 | 2016-03-18 | 检测水稻半矮杆基因sd-1等位基因用的CAPS标记 |
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CN201610156855.2A CN107201396B (zh) | 2016-03-18 | 2016-03-18 | 检测水稻半矮杆基因sd-1等位基因用的CAPS标记 |
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CN107201396A true CN107201396A (zh) | 2017-09-26 |
CN107201396B CN107201396B (zh) | 2020-11-27 |
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CN201610156855.2A Expired - Fee Related CN107201396B (zh) | 2016-03-18 | 2016-03-18 | 检测水稻半矮杆基因sd-1等位基因用的CAPS标记 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110894542A (zh) * | 2019-12-31 | 2020-03-20 | 扬州大学 | 一种鉴别水稻gs5基因和glw7基因类型的引物及其应用 |
CN116334290A (zh) * | 2023-04-12 | 2023-06-27 | 湖北省农业科学院粮食作物研究所 | 一种鉴定水稻功能基因的引物组、试剂盒及应用 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003070934A1 (fr) * | 2002-02-25 | 2003-08-28 | Plant Genome Center Co., Ltd. | Methode d'analyse du genotype de la region situee autour du gene sd-1 vegetal et methode d'analyse des caracteristiques d'un vegetal semi-nain utilisant la methode d'analyse du genotypage |
CN101440374A (zh) * | 2001-06-19 | 2009-05-27 | 本田技研工业株式会社 | 涉及植物半矮化的sd1基因和其应用 |
CN102174519A (zh) * | 2011-02-25 | 2011-09-07 | 中国农业科学院作物科学研究所 | 一种矮败水稻培育方法及其所用到的dna |
CN102994635A (zh) * | 2012-06-21 | 2013-03-27 | 浙江省农业科学院 | 检测水稻粒型的分子标记方法、试剂盒和引物 |
CN104630364A (zh) * | 2015-02-05 | 2015-05-20 | 中国科学院遗传与发育生物学研究所 | 抗稻瘟病基因Pi9的特异性CAPS标记Pi9caps及其应用 |
-
2016
- 2016-03-18 CN CN201610156855.2A patent/CN107201396B/zh not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101440374A (zh) * | 2001-06-19 | 2009-05-27 | 本田技研工业株式会社 | 涉及植物半矮化的sd1基因和其应用 |
WO2003070934A1 (fr) * | 2002-02-25 | 2003-08-28 | Plant Genome Center Co., Ltd. | Methode d'analyse du genotype de la region situee autour du gene sd-1 vegetal et methode d'analyse des caracteristiques d'un vegetal semi-nain utilisant la methode d'analyse du genotypage |
CN102174519A (zh) * | 2011-02-25 | 2011-09-07 | 中国农业科学院作物科学研究所 | 一种矮败水稻培育方法及其所用到的dna |
CN102994635A (zh) * | 2012-06-21 | 2013-03-27 | 浙江省农业科学院 | 检测水稻粒型的分子标记方法、试剂盒和引物 |
CN104630364A (zh) * | 2015-02-05 | 2015-05-20 | 中国科学院遗传与发育生物学研究所 | 抗稻瘟病基因Pi9的特异性CAPS标记Pi9caps及其应用 |
Non-Patent Citations (2)
Title |
---|
A. SASAKI等: "A mutant gibberellin -synthesis gene in rice", 《NATURE》 * |
吴秀兰等: "单核苷酸多态性在水稻遗传育种中的应用", 《分子植物育种》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110894542A (zh) * | 2019-12-31 | 2020-03-20 | 扬州大学 | 一种鉴别水稻gs5基因和glw7基因类型的引物及其应用 |
CN116334290A (zh) * | 2023-04-12 | 2023-06-27 | 湖北省农业科学院粮食作物研究所 | 一种鉴定水稻功能基因的引物组、试剂盒及应用 |
CN116334290B (zh) * | 2023-04-12 | 2024-04-05 | 湖北省农业科学院粮食作物研究所 | 一种鉴定水稻功能基因的引物组、试剂盒及应用 |
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