CN107198240A - 黑果枸杞中多种有效成分的联合提取方法 - Google Patents
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Abstract
本发明涉及一种黑果枸杞中多糖、色素、蛋白质、籽油和籽饼的联合提取方法。主体工艺包括研磨、水提、醇沉、水溶、浓缩等步骤。将黑果枸杞挑选后研磨，先后经50℃水提(pH3)和90℃水提，合并两次提取液，离心，从渣中分离出黑果枸杞籽，经烘干、压榨获得黑果枸杞籽油和黑果枸杞籽饼产品；将上清液醇沉后过滤得清液和沉淀物，将清液浓缩后冷冻干燥得黑果枸杞色素产品；将沉淀加水溶解，不溶物即为蛋白质，将水溶液再次醇沉、干燥即得黑果枸杞多糖产品。本发明工艺简便，可同时得到黑果枸杞中的色素、多糖、蛋白质、籽油和籽饼产品，用于抗氧化、预防心血管疾病等保健品的原料，从而高值化综合利用黑果枸杞。
Description
技术领域：
本发明涉及一种黑果枸杞高值化综合利用的方法，将黑果枸杞中色素、枸杞多糖、蛋白质和枸杞籽联合提取。
背景技术：
黑果枸杞中含有丰富的原花青素、多糖、蛋白质等各种营养成分。其中，原花青素是最有效的天然自由基清除剂，其功效是维生素C的20倍、VE的50倍；枸杞多糖具有抗疲劳、降血糖、增强免疫力等生理功能，黑果枸杞蛋白质氨基酸组成符合WHO/FAO的参考蛋白模式，属于优质蛋白。其药用保健价值远远高于普通红枸杞，被誉为“软黄金”。
目前，有多种方法用于提取黑果枸杞中的色素或多糖，如乌鲁木齐益广康源生物科技有限公司申报的“黑果枸杞果实提取花青素及其制备方法”专利(申请号：201510128567.1申请公布号：CN 104789612 A)，青海康元药用资源科技利用有限公司申报的“一种黑果枸杞固液隔离提取多糖的方法”专利(申请号：201610100244.6申请公布号：CN105542029 A)。现有方法只能对其中的一种物质进行单独的提取，而且在提取工艺过程对其它成分有一定程度破坏，从而造成了黑果枸杞的浪费。
本发明对黑果枸杞中多种有效成分进行联合提取，产品可用于制备高附加值的食品和保健品，可大大提高黑果枸杞的资源利用率。
发明内容：
本发明所要解决的技术问题是提供一种联合提取黑果枸杞中的色素、多糖、蛋白质和枸杞籽的方法。本发明解决上述技术问题所采用的技术方案为：将黑果枸杞干果充分研磨后按1∶50的固液比于50℃ pH3的盐酸溶液浸提150分钟。离心后收集沉淀按1∶50的固液比于90℃水浸提30分钟。再次离心使固液分离，从沉淀中收集得黑果枸杞籽，经烘干、压榨可获得黑果枸杞籽油和黑果枸杞籽饼；合并两次提取液，真空浓缩后加入95％乙醇，使乙醇浓度达到80～85％，4℃下静置过夜后过滤得上清液和沉淀，将上清液进行真空浓缩回收乙醇后冷冻干燥得黑果枸杞色素产品；将沉淀用水溶解，过滤收集不溶物、干燥即得黑果枸杞蛋白质，水溶液进行二次醇沉(乙醇浓度达到80～85％)、过滤、干燥即得黑果枸杞多糖产品。
与现有技术相比，本发明的优点在于：工艺简便、得率高，实现了黑果枸杞中各种有效成分的联合提取，使原料得到了最大限度的利用，减少了黑果枸杞资源浪费。
附图说明：
图1是黑果枸杞各种成分联合提取的工艺流程。
具体实施方法：
实例：取5kg黑果枸杞干果充分研磨后按1∶50的固液比加入pH3的盐酸溶液于50℃浸提150分钟。离心使固液分离，收集沉淀按1∶50的固液比加入蒸馏水250L于90℃浸提30分钟，再次离心使固液分离。收集沉淀中的枸杞籽1kg，烘干后压榨获得黑果枸杞籽油和黑果枸杞籽饼；将两次浸提上清液合并、真空浓缩，加入95％的乙醇调至80％，4℃下静置过夜醇沉后过滤，将上清液进行真空浓缩后进行冷冻干燥即得黑果枸杞色素产品2.92kg。滤渣用水溶解后，离心得到不溶性物质即为蛋白质(干重42g)，上清液加95％的乙醇调至80％进行二次醇沉，过滤，收集沉淀、干燥即得黑果枸杞多糖116g。
Claims (2)
1.黑果枸杞中多种有效成分的联合提取方法，其特征在于，将黑果枸杞中黑果枸杞色素、黑果枸杞多糖、黑果枸杞蛋白质、黑果枸杞籽油、黑果枸杞籽饼依次提取出来，实现了黑果枸杞的综合利用，且工艺简便易行。
2.黑果枸杞中多种有效成分的联合提取方法，其步骤为：
(1)将黑果枸杞干果充分研磨，用pH＝3的盐酸溶液按照1∶50的固液比，50℃下浸提150min；
(2)将步骤(1)所得混合溶液离心，分别得到上清液和沉淀；
(3)将步骤(2)所得沉淀用蒸馏水按1∶50固液比，90℃浸提30min；
(4)将步骤(3)所得混合液离心得上清液和沉淀，收集沉淀再经干燥即得黑果枸杞籽；
(5)将步骤(4)所得黑枸杞籽压榨，即得黑枸杞籽油和黑枸杞籽饼；
(6)将步骤(2)和步骤(4)所述的上清液合并，真空浓缩；
(7)向步骤(6)所得浓缩液中加入95％的乙醇使乙醇浓度达到80～85％，4℃下静置过夜；
(8)将步骤(7)所得混合物过滤得上清液和沉淀；
(9)将步骤(8)所得上清液经真空浓缩回收乙醇后冷冻干燥即得黑果枸杞色素产品；
(10)将步骤(8)所得沉淀以蒸馏水溶解后离心，得上清液和沉淀；
(11)将步骤(10)中上清液加入95％的乙醇使乙醇浓度达到80～85％，4℃下静置过夜；
(12)将步骤(11)所得混合物过滤得上清液和沉淀；
(13)将步骤(12)中所得沉淀经干燥即得黑果枸杞多糖产品；
(14)将步骤(10)中所得沉淀干燥即得黑果枸杞蛋白质产品。
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110746515A (zh) * | 2019-10-28 | 2020-02-04 | 百瑞源枸杞股份有限公司 | 一种同步分离制取的枸杞多糖、枸杞红素、枸杞多肽及其制备方法 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110746515A (zh) * | 2019-10-28 | 2020-02-04 | 百瑞源枸杞股份有限公司 | 一种同步分离制取的枸杞多糖、枸杞红素、枸杞多肽及其制备方法 |
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Application publication date: 20170926 |