CN107188655A - The cultivation of auricularia auricula generation material and cultural method of a kind of useless branch of utilization grape - Google Patents

The cultivation of auricularia auricula generation material and cultural method of a kind of useless branch of utilization grape Download PDF

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Publication number
CN107188655A
CN107188655A CN201710547766.5A CN201710547766A CN107188655A CN 107188655 A CN107188655 A CN 107188655A CN 201710547766 A CN201710547766 A CN 201710547766A CN 107188655 A CN107188655 A CN 107188655A
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Prior art keywords
black fungus
bacterium
cultivation
branch
rod
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CN201710547766.5A
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Inventor
周晓武
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Zhuji Spring Edible Mushroom Cultivation Farm
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Zhuji Spring Edible Mushroom Cultivation Farm
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Priority to CN201710547766.5A priority Critical patent/CN107188655A/en
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00—Calcareous fertilisers

Abstract

The present invention discloses the cultivation of auricularia auricula generation material and cultural method of a kind of useless branch of utilization grape.The present invention gives up branch as wherein raw material by the use of grape and makes bacterium rod, then carries out autoclaving → bacterium inoculation → culture, induces former base, when there is black fungus former base in bacterium rod bag wall, ear hole is outputed in whole black fungus bacterial rod wall surrounding, increases oxygen, promotes the growth of black fungus bud；Finally it is colonized down, produce agaric.The black fungus bacterial rod that the present invention is prepared is more ventilative compared with prior art, beneficial to mycelial growth, is favorably improved black fungus yield；So that the black fungus bacteria time greatly shortens, grow in advance of about 15 days.

Description

The cultivation of auricularia auricula generation material and cultural method of a kind of useless branch of utilization grape
Technical field
The invention belongs to technical field of crop cultivation, be related to a kind of utilization grape give up branch cultivation of auricularia auricula generation material with And cultural method.
Background technology
Black fungus is a kind of delicious glial mushroom and medicinal fungus of fine quality, and China's black fungus producing region is divided into three：Northeast Piece --- Liaoning, Jilin, Heilungkiang.Central China piece --- Shaanxi, Shanxi, Gansu, Sichuan, Henan, Hebei, Hubei.South Piece --- Yunnan, Guizhou, Guangxi, Guangdong, Hunan, Fujian, Taiwan, Jiangxi, Shanghai.Black fungus meat is fine and smooth, crisp smooth mouth, battalion Support abundant.Its protein content is high more than general vegetables and fruit.And contain amino acid and multivitamin necessary to the mankind. The content of wherein vitamin B be rice, face, ten times of vegetables, it is higher than meat 3-6 times.The content of irony is higher than meat 100 times.Calcium Content be 30-70 times of meat, the content of phosphorus is also higher than egg, meat, be tomato, 4-7 times of potato.Black fungus has Have moist strong, clearing lung-heat QI invigorating, sedation-analgesia, the effects such as washing stomach and intestine clearly, the polysaccharide body contained by it also has significant antitumor Activity；The traditional Chinese medical science treatment cold-damp lumbocrural pain, brothers knot numbness, hemorrhoid hemorrhage, dysentery, collapse and drench and the disease such as puerperal asthenia The common prescription medicine of disease；It is the health food of weaving, cement, mining, cleaner and chemical plant workman.Nucleosides in black fungus Acid can reduce the cholesterol level in blood, therefore, US business in 1980 advise China black fungus wear into powdery or Grain enters American market as medicine.Their prospect for sales to black fungus as medicine in the U.S. are very optimistic, because heart Turn into the No.1 disease in the U.S. with the disease in terms of blood vessel.There is research in China very early to the medicinal of black fungus, during Ming Dynasty Lee Precious《Compendium of Materia Medica》In write：" ... disconnected paddy controls hemorrhoid ..., metrorrhagia and metrostaxis, blood under bloody flux ".
Because black fungus has higher nutritive value and medical value, so be all in great demand very much in domestic and international market, product Supply falls short of demand.China's black fungus yield occupies the first place in the world, is traditional large outlet special product, enjoys in the international market very high Reputation.Therefore, natural forest tree resource and agricultural crop straw rationally using mountain area is fully excavated, black fungus life is greatly developed Production, to revitalizing local economy, expanding foreign export has important realistic meaning.
In early stage, the black fungus producing region of China is that, using the cultivation of old method, have plenty of the natural biography by means of black fungus spore Broadcast；Have plenty of and spread by means of the mycelia of old ear wood；Have plenty of using wood fragments ear to be inoculated with.The fifties, China's research-on-research Person passes through painstaking efforts, successfully cultivates pure culture, and applied to production, change Semi-artificial cultivation shape for a long time State, not only shortens the production cycle of black fungus, and yield also obtain growth at double, and quality is also significantly increased.
Since the seventies, the domestic research for having carried out substituting stuff cultivation black fungus again, applied to production.Black fungus generation Material cultivation is using wood chip, with the container culturing black fungus such as polybag.Substituting stuff cultivation aboundresources, yield is high, and the cycle is short, is one Plant promising cultural method.
Because Bag Material cultivation of auricularia auricula raw material mainly utilize weed tree sawdust, and weed tree sawdust needs external allocation and transportation, cost high and Destruction is ecological.The applicant carries out cultivating black fungus using the useless branch of grape, implements black fungus scale high yield substituting stuff cultivation, no It is only capable of improving the yield of black fungus product to a certain extent, while to promoting wasted resources, developing a circular economy, cleans The earth, beautiful rural area is significant.
The content of the invention
It is an object of the invention to a kind of black wood for the branch that given up in view of the shortcomings of the prior art there is provided utilization grape Ear cultivation generation material.
Cultivation of auricularia auricula generation material of the present invention includes each component of following content of material：
Further optimization：
It is a further object to provide a kind of cultivation of auricularia auricula method of the useless branch of utilization grape.
This method comprises the following steps：
Step (1), select bag
Using 18cm × 55cm Polypropylene Bags；
Step (2), charging
Weed tree sawdust, the useless branch of grape, Mai Fu, land plaster, sugar are stirred in above-mentioned Polypropylene Bag, water is added so that Material moisture 55-60% (content of material), at the same adjust pH value be 6.5-7.0, sack tighten after as black fungus bacterial rod；
By each component of following content of material：
Further optimization：
Step (3), autoclaving → bacterium inoculation → culture
The black fungus bacterial rod that step (2) is prepared handles 18- under the conditions of 100-105 DEG C of high pressure (18-19MPa) 22h；
Million grades of desinfection chamber inoculations：In black fungus bacterial rod wall three bacterium eyes (depth 3-4cm, hole are opened along longitudinal straight uniform Footpath 2-3cm), bacterium rod two ends bacterium eye is 2-4cm with bacterium stick end distance；Then black fungus strain is filled in above-mentioned bacterium eye；
Condition of culture：Lucifuge culture 40~45 days at 22-25 DEG C are put, until mycelia is covered with whole bacterium rod；
Step (4), induce former base
After mycelia purseful, 18-20 DEG C is cooled to, ventilation, printing opacity culture induce former base；
Step (5), punching ventilation
When about there is black fungus former base in bacterium rod bag wall through one week after step (4), opened in whole black fungus bacterial rod wall surrounding Go out ear hole, increase oxygen, promote the growth of black fungus bud；
Further, ear hole requirement is outputed：Adjacent hole is apart from 2-2.5cm, hole depth 0.5-1cm, aperture 0.2-0.3cm；
Step (6), lower it is colonized
Step (5) outputs ear hole after one week when mycelia covers with out ear hole, and selection continuous sunny is cultivated for one week at present；
Further, cultivate lowerly and tiltedly stand on ground for bacterium rod, angle is 60-80 °；
Step (7), ear management
In the case where intensity is 800-1000lx illumination, young ear is gone out using 15-25 DEG C of thermal stimulation formation former base of daytime；Go out young ear Water spray keeps humidity 80-90% (mass content) when daily more than 6 hours long afterwards, until black fungus is ripe.
The beneficial effects of the invention are as follows：
1st, the present invention utilizes the useless branch of grape so that black fungus product fine, yield is high, and benefit is stable.
2nd, the black fungus bacterial rod that the present invention is prepared is more ventilative compared with prior art, beneficial to mycelial growth, helps to carry High black fungus yield.
3rd, raw material of the present invention to promoting wasted resources, are developed a circular economy, cleaned using the useless branch of substantial amounts of grape The earth, beautiful rural area is significant.
4th, using cultivation of auricularia auricula method of the present invention so that the black fungus bacteria time greatly shortens, grow in advance of about 15 days.
5th, compare prior art, and the spring ear quality that the present invention can be prepared is higher, and yield is higher.
The bacterium rod that prior art is used easily is degenerated in spring so that spring ear is malnutritive, and yield is extremely low.And it is of the invention Overcome the shortcoming.
Embodiment
The present invention is further analyzed below in conjunction with specific embodiment.
Embodiment 1：
In lunar calendar July, Zhejiang Province's Zhuji City spring profit edible fungus culturing base carries out black fungus rod.Lunar calendar July system Bacterium rod kind processed, the lunar calendar eight, nine, moon bacterium germination are got over the summer, are colonized under the deflation oxygenation of lunar calendar October, and the lunar calendar starts receipts mushroom December.
This method comprises the following steps：
Step (1), select bag
Using 18cm × 55cm Polypropylene Bags；
Step (2), charging
By 687.5g weed tree sawdusts, 400g grapes useless branch, 150g wheats husband, 10g land plasters, 2.5g in above-mentioned Polypropylene Bag Sugar stirs, and adds water so that material moisture 55% (content of material), while it is 6.5 to adjust pH value, sack is made after tightening For black fungus bacterial rod；
Step (3), normal-pressure sterilization → bacterium inoculation → culture
The black fungus bacterial rod that step (2) is prepared handles 22h under the conditions of 18MPa, 100 DEG C；
Million grades of desinfection chamber inoculations：In black fungus bacterial rod wall three bacterium eyes (depth 3cm, aperture are opened along longitudinal straight uniform 3cm), bacterium rod two ends bacterium eye and bacterium stick end distance are 2cm；Then black fungus strain is filled in above-mentioned bacterium eye；
Condition of culture：Lucifuge culture 45 days at 22 DEG C are put, until mycelia is covered with whole bacterium rod；
Step (4), induce former base
After mycelia purseful, 18 DEG C are cooled to, ventilation, printing opacity culture induce former base；
Step (5), punching ventilation
When about there is black fungus former base in bacterium rod bag wall through one week after step (4), opened in whole black fungus bacterial rod wall surrounding Go out ear hole, increase oxygen, promote the growth of black fungus bud；
Output ear hole requirement：Adjacent hole is apart from 2cm, hole depth 1cm, aperture 0.3cm；
Step (6), lower it is colonized
Step (5) outputs ear hole after one week when mycelia covers with out ear hole, and selection continuous sunny is cultivated for one week at present；
Cultivate lowerly and tiltedly stand on ground for bacterium rod, angle is 60 °；
Step (7), ear management
In the case where intensity is 1000lx illumination, young ear is gone out using 15-25 DEG C of thermal stimulation formation former base of daytime；Go out every after young ear Water spray keeps humidity 90% (mass content) when it is more than 6 hours long, until black fungus is ripe.
Embodiment 2：
In lunar calendar July, Zhejiang Province's Zhuji City spring profit edible fungus culturing base carries out black fungus rod.Lunar calendar July system Bacterium rod kind processed, the lunar calendar eight, nine, moon bacterium germination are got over the summer, are colonized under the deflation oxygenation of lunar calendar October, and the lunar calendar starts receipts mushroom December.
This method comprises the following steps：
Step (1), select bag
Using 18cm × 55cm Polypropylene Bags；
Step (2), charging
By 750g weed tree sawdusts, 312.5g grapes useless branch, 150g wheats husband, 30g land plasters, 7.5g in above-mentioned Polypropylene Bag Sugar is stirred, and adds water so that material moisture 60% (content of material), while it is 6.5-7.0 to adjust pH value, sack is tightened Afterwards as black fungus bacterial rod；
Step (3), normal-pressure sterilization → bacterium inoculation → culture
The black fungus bacterial rod that step (2) is prepared handles 18h under the conditions of high pressure 19MPa, 105 DEG C；
Million grades of desinfection chamber inoculations：In black fungus bacterial rod wall three bacterium eyes (depth 4cm, aperture are opened along longitudinal straight uniform 2cm), bacterium rod two ends bacterium eye and bacterium stick end distance are 4cm；Then black fungus strain is filled in above-mentioned bacterium eye；
Condition of culture：Lucifuge culture 40 days at 25 DEG C are put, until mycelia is covered with whole bacterium rod；
Step (4), induce former base
After mycelia purseful, 20 DEG C are cooled to, ventilation, printing opacity culture induce former base；
Step (5), punching ventilation
When about there is black fungus former base in bacterium rod bag wall through one week after step (4), opened in whole black fungus bacterial rod wall surrounding Go out ear hole, increase oxygen, promote the growth of black fungus bud；
Output ear hole requirement：Adjacent hole is apart from 2.5cm, hole depth 0.5cm, aperture 0.3cm；
Step (6), lower it is colonized
Step (5) outputs ear hole after one week when mycelia covers with out ear hole, and selection continuous sunny is cultivated for one week at present；
Cultivate lowerly and tiltedly stand on ground for bacterium rod, angle is 80 °；
Step (7), ear management
In the case where intensity is 900lx illumination, young ear is gone out using 15-25 DEG C of thermal stimulation formation former base of daytime；Go out every after young ear Water spray keeps humidity 85% (mass content) when it is more than 6 hours long, until black fungus is ripe.
Embodiment 3：
In lunar calendar July, Zhejiang Province's Zhuji City spring profit edible fungus culturing base carries out black fungus rod.Lunar calendar July system Bacterium rod kind processed, the lunar calendar eight, nine, moon bacterium germination are got over the summer, are colonized under the deflation oxygenation of lunar calendar October, and the lunar calendar starts receipts mushroom December.
This method comprises the following steps：
Step (1), select bag
Using 18cm × 55cm Polypropylene Bags；
Step (2), charging
725g weed tree sawdusts, the useless branch of 375g grapes, 125g wheats husband, 10g land plasters, 15g sugar are stirred in above-mentioned Polypropylene Bag Mix uniform, add water so that material moisture 58% (content of material), while it is 7.0 to adjust pH value, sack tighten after as black Agaric fungus rod；
Step (3), normal-pressure sterilization → bacterium inoculation → culture
The black fungus bacterial rod that step (2) is prepared handles 20h under the conditions of high pressure 19MPa, 102 DEG C；
Million grades of desinfection chamber inoculations：In black fungus bacterial rod wall three bacterium eyes (depth 3.5cm, hole are opened along longitudinal straight uniform Footpath 2.5cm), bacterium rod two ends bacterium eye is 3cm with bacterium stick end distance；Then black fungus strain is filled in above-mentioned bacterium eye；
Condition of culture：Lucifuge culture 43 days at 23 DEG C are put, until mycelia is covered with whole bacterium rod；
Step (4), induce former base
After mycelia purseful, 19 DEG C are cooled to, ventilation, printing opacity culture induce former base；
Step (5), punching ventilation
When about there is black fungus former base in bacterium rod bag wall through one week after step (4), opened in whole black fungus bacterial rod wall surrounding Go out ear hole, increase oxygen, promote the growth of black fungus bud；
Output ear hole requirement：Adjacent hole is apart from 2.2cm, hole depth 0.8cm, aperture 0.25cm；
Step (6), lower it is colonized
Step (5) outputs ear hole after one week when mycelia covers with out ear hole, and selection continuous sunny is cultivated for one week at present；
Cultivate lowerly and tiltedly stand on ground for bacterium rod, angle is 70 °；
Step (7), ear management
In the case where intensity is 1000lx illumination, young ear is gone out using 15-25 DEG C of thermal stimulation formation former base of daytime；Go out every after young ear Water spray keeps humidity 90% (mass content) when it is more than 6 hours long, until black fungus is ripe.
Embodiment 4：
Step (1), select bag
Using 18cm × 55cm Polypropylene Bags；
Step (2), charging
By 750g weed tree sawdusts, the useless branch of 387.5g grapes, 100g wheats husband, 5g land plasters, 7.5g sugar in above-mentioned Polypropylene Bag Stir, add water so that material moisture 60% (content of material), while it is 6.5, conduct after sack is tightened to adjust pH value Black fungus bacterial rod；
Step (3), normal-pressure sterilization → bacterium inoculation → culture
The black fungus bacterial rod that step (2) is prepared handles 21h under the conditions of high pressure 18MPa, 104 DEG C；
Million grades of desinfection chamber inoculations：In black fungus bacterial rod wall three bacterium eyes (depth 4cm, aperture are opened along longitudinal straight uniform 3cm), bacterium rod two ends bacterium eye and bacterium stick end distance are 4cm；Then black fungus strain is filled in above-mentioned bacterium eye；
Condition of culture：Lucifuge culture 40 days at 25 DEG C are put, until mycelia is covered with whole bacterium rod；
Step (4), induce former base
After mycelia purseful, 18 DEG C are cooled to, ventilation, printing opacity culture induce former base；
Step (5), punching ventilation
When about there is black fungus former base in bacterium rod bag wall through one week after step (4), opened in whole black fungus bacterial rod wall surrounding Go out ear hole, increase oxygen, promote the growth of black fungus bud；
Output ear hole requirement：Adjacent hole is apart from 2cm, hole depth 1cm, aperture 0.3cm；
Step (6), lower it is colonized
Step (5) outputs ear hole after one week when mycelia covers with out ear hole, and selection continuous sunny is cultivated for one week at present；
Further, cultivate lowerly and tiltedly stand on ground for bacterium rod, angle is 75 °；
Step (7), ear management
In the case where intensity is 800lx illumination, young ear is gone out using 15-25 DEG C of thermal stimulation formation former base of daytime；Go out every after young ear Water spray keeps humidity 80% (mass content) when it is more than 6 hours long, until black fungus is ripe.
The black fungus yield that above-described embodiment 1-4 is prepared is that each bacterium rod produces 180-250kg, and prior art can only Produce 150-200kg.And the black fungus that above-described embodiment is prepared is more soft more tasty and more refreshing than former mouthfeel, and meat is more abundant, Color is pitch black.
Above-described embodiment black fungus maturity period is longer of about 15 days than in advance with prior art.
Above-described embodiment is not the limitation for the present invention, and the present invention is not limited only to above-described embodiment, as long as meeting Application claims, belong to protection scope of the present invention.

Claims (10)

  1. The cultivation of auricularia auricula generation material of branch 1. a kind of utilization grape gives up, it is characterised in that include each component of following content of material：
  2. The cultivation of auricularia auricula generation material of branch 2. a kind of utilization grape as claimed in claim 1 gives up, it is characterised in that including following The each component of content of material：
  3. A kind of cultivation of auricularia auricula method of branch 3. utilization grape gives up, it is characterised in that this method comprises the following steps：
    Step (1), select bag
    Using 18cm × 55cm Polypropylene Bags；
    Step (2), charging
    Weed tree sawdust, the useless branch of grape, Mai Fu, land plaster, sugar are stirred in above-mentioned Polypropylene Bag, water is added so that material Water content 55-60%, at the same adjust pH value be 6.5-7.0, sack tighten after as black fungus bacterial rod；
    The content of material of above-mentioned each component is as follows：
    Step (3), autoclaving → bacterium inoculation → culture
    The black fungus bacterial rod that step (2) is prepared handles 18-22h under the conditions of 100-105 DEG C of high pressure；
    Bacterium rod after above-mentioned processing is placed in into million grades of desinfection chambers to be inoculated with：Opened in black fungus bacterial rod wall along longitudinal straight uniform Three bacterium eyes, are then filled in above-mentioned bacterium eye by black fungus strain；
    Condition of culture：Lucifuge culture 40~45 days at 22-25 DEG C are put, until mycelia is covered with whole bacterium rod；
    Step (4), induce former base
    After mycelia purseful, 18-20 DEG C is cooled to, ventilation, printing opacity culture induce former base；
    Step (5), punching ventilation
    When about there is black fungus former base in bacterium rod bag wall through one week after step (4), ear is outputed in whole black fungus bacterial rod wall surrounding Hole, increases oxygen, promotes the growth of black fungus bud；
    Step (6), lower it is colonized
    Step (5) outputs ear hole after one week when mycelia covers with out ear hole, and selection continuous sunny is cultivated for one week at present；
    Step (7), ear management
    Under illumination, young ear is gone out using 15-25 DEG C of thermal stimulation formation former base of daytime；Sprayed when going out daily more than 6 hours long after young ear Water keeps humidity 80-90%, until black fungus is ripe.
  4. The cultivation of auricularia auricula method of branch 4. a kind of utilization grape as claimed in claim 3 gives up, it is characterised in that step (2) is each The content of material of component is as follows：
  5. The cultivation of auricularia auricula method of branch 5. a kind of utilization grape as claimed in claim 3 gives up, it is characterised in that step (3) is high The pressure that pressure sterilizing is used is 18-19MPa.
  6. The cultivation of auricularia auricula method of branch 6. a kind of utilization grape as claimed in claim 3 gives up, it is characterised in that step (3) bacterium The depth 3-4cm, aperture 2-3cm of eye.
  7. The cultivation of auricularia auricula method of branch 7. a kind of utilization grape as claimed in claim 3 gives up, it is characterised in that step (3) bacterium Rod two ends bacterium eye and bacterium stick end distance are 2-4cm.
  8. The cultivation of auricularia auricula method of branch 8. a kind of utilization grape as claimed in claim 3 gives up, it is characterised in that step (5) is opened Go out ear hole requirement：Adjacent hole is apart from 2-2.5cm, hole depth 0.5-1cm, aperture 0.2-0.3cm.
  9. The cultivation of auricularia auricula method of branch 9. a kind of utilization grape as claimed in claim 3 gives up, it is characterised in that under step (6) Ground cultivation tiltedly stands on ground for bacterium rod, and angle is 60-80 °.
  10. The cultivation of auricularia auricula method of branch 10. a kind of utilization grape as claimed in claim 3 gives up, it is characterised in that step (7) Intensity of illumination is 800-1000lx.
CN201710547766.5A 2017-07-06 2017-07-06 The cultivation of auricularia auricula generation material and cultural method of a kind of useless branch of utilization grape Pending CN107188655A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107624515A (en) * 2017-10-24 2018-01-26 建瓯市元润塑料有限公司 Black fungus open air cultivating bacteria bag and its preparation and application method
CN111480515A (en) * 2020-05-13 2020-08-04 桐梓县马鬃苗岭菌业有限公司 Oyster mushroom planting method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102815978A (en) * 2011-06-07 2012-12-12 林金榜 Edible fungus culture medium with grape branches as main raw material, and edible fungus culturing method
CN104557270A (en) * 2014-12-19 2015-04-29 新林区鲲鹏食用菌农民专业合作社 Black fungus culture method and culture medium suitable for northeast region
CN104973958A (en) * 2015-06-25 2015-10-14 广西柳城县成霖农业科技有限公司 Black fungus culture substrate and preparation method therefor
CN105859415A (en) * 2016-04-14 2016-08-17 上海大学 Novel auricularia auricula culture material and preparation method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102815978A (en) * 2011-06-07 2012-12-12 林金榜 Edible fungus culture medium with grape branches as main raw material, and edible fungus culturing method
CN104557270A (en) * 2014-12-19 2015-04-29 新林区鲲鹏食用菌农民专业合作社 Black fungus culture method and culture medium suitable for northeast region
CN104973958A (en) * 2015-06-25 2015-10-14 广西柳城县成霖农业科技有限公司 Black fungus culture substrate and preparation method therefor
CN105859415A (en) * 2016-04-14 2016-08-17 上海大学 Novel auricularia auricula culture material and preparation method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107624515A (en) * 2017-10-24 2018-01-26 建瓯市元润塑料有限公司 Black fungus open air cultivating bacteria bag and its preparation and application method
CN111480515A (en) * 2020-05-13 2020-08-04 桐梓县马鬃苗岭菌业有限公司 Oyster mushroom planting method

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Application publication date: 20170922