CN107167558A - A kind of additive agent for feeding replace anti-performance appraisal procedure - Google Patents
A kind of additive agent for feeding replace anti-performance appraisal procedure Download PDFInfo
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- CN107167558A CN107167558A CN201710364004.1A CN201710364004A CN107167558A CN 107167558 A CN107167558 A CN 107167558A CN 201710364004 A CN201710364004 A CN 201710364004A CN 107167558 A CN107167558 A CN 107167558A
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- appraisal procedure
- feeding
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- diarrhoea
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
Abstract
The invention provides a kind of additive agent for feeding replace anti-performance appraisal procedure, by gavage diarrhoea animal, assess animal rehabilitation rate with assess the additive agent for feeding replace anti-performance.The appraisal procedure that the present invention is provided changes traditional evaluation profile, can significantly shorten the assessment time, without large-scale farming site, greatly reduces the cost of assessment, improves efficiency, and assessment result is close with Traditional measurements method, as a result accurately, reliably.The appraisal procedure of the present invention can be used alone, and can act also as primary dcreening operation step and is used with Traditional measurements Combination of Methods.
Description
Technical field
The present invention relates to feed additive field, and in particular to a kind of additive agent for feeding replace anti-performance appraisal procedure.
Background technology
Grice diarrhoea (diarrhea) is a bottleneck for restricting pig industry development, and huge economic damage is brought to pig industry
Lose.Grice diarrhoea is mainly the bacterial diarrhea as caused by Escherichia coli, salmonella and clostridieum welchii, most often greatly
Enterobacteria and salmonellal yellow dysentery and dysentery characterized by white mucous stool.Treatment grice diarrhoea is usually using antibiotic at present, but antibiotic
Drug resistance and medicament residue have been beaten in alarm bell, therapeutic process for the use of antibiotics and there is side effect, easily cause
Food security addiction is suffered from, and the Ministry of Agriculture will completely forbid and antibiotic is added in animal feed, therefore exploitation new antibiotic substitute
It is extremely urgent.
At present, declare that the additive product of alternative antibiotic emerges in an endless stream on the market, including plant extracts, bacteriolyze
Multiple species such as enzyme, acidulant, not only species is various, and aspect of performance is also that the good and bad jumbled together, it is therefore necessary to have to its performance
Effect is assessed and just can confirm that whether it can reach antibiotic substitution effect.Traditional for anti-performance appraisal procedure is added in feed
Additive to be assessed, investigates growth of animal performance after feeding, this appraisal procedure cycle length, it is necessary to large-scale experiment place,
Assessed cost is high, therefore, it is that feed is ground that the antibiotic of a kind of low cost of exploitation, quick, accurate evaluation additive agent for feeding is alternative
Study carefully an important topic in field.
The content of the invention
To overcome the defect that existing additive agent for feeding exists in terms of antibiotic substitution effect is assessed, the purpose of the present invention
Be to provide a kind of additive agent for feeding replaces anti-performance appraisal procedure.
The present invention additive agent for feeding be for anti-performance appraisal procedure：Additive agent for feeding to be assessed is gavaged into diarrhoea dynamic
Thing, by assessing animal rehabilitation rate with assess the additive agent for feeding replace anti-performance.
On the whole, traditional feeding animals pattern is changed into and more easy directly gavaged by appraisal procedure of the invention
The pattern of diarrhoea animal, changes into the investigation of rehabilitation situation by the investigation of growth performance, fundamentally changes traditional addition
Anti-performance Evaluation example is replaced in agent.On this basis, those skilled in the art may also be combined with specific cultivation situation, such as humiture shadow
Sound, animal itself difference (kind, body weight etc.), column home ambient influnence etc. make appropriate adjustment, exclude adverse effect factor.
The present invention appraisal procedure need to exclude gavage animal dis other epidemic situations, especially viral epidemic situation, so as to replace
Anti-performance assessment result is more accurate and reliable.
In a preferred embodiment of the appraisal procedure according to the present invention, the appraisal procedure includes following mistake
Journey：Additive agent for feeding to be assessed is gavaged into diarrhoea animal, animal rehabilitation rate is assessed, reaches that 50% is indicated above described to be assessed
Additive agent for feeding possess for anti-performance.
In another preferred embodiment of the appraisal procedure according to the present invention, the appraisal procedure includes following mistake
Journey：Additive agent for feeding to be assessed is gavaged into diarrhoea animal, diarrhoea animal is gavaged as a control group using antibiotic, animal is assessed
Rehabilitation rate, reaches that the control group gavages 70% additive agent for feeding to be assessed indicated above of animal rehabilitation rate and possesses and replace
Anti-performance.
Antibiotic, for anti-product for treatment suffer from diarrhoea animal rehabilitation situation and differ, or even there are greatest differences,
Under this situation, it can be commented using existing antibiotic product as a control group by contrasting the rehabilitation situation of investigation diarrhoea animal
Estimate for anti-performance, cover the shortage.
, can also be by additive agent for feeding to be assessed in another preferred embodiment of the appraisal procedure according to the present invention
Animal rehabilitation rate investigate the integral rehabilitation situation of animal of gavaging with respect to rehabilitation rate in combination with control group.
In the embodiment of above-mentioned appraisal procedure, antibiotic control group can select existing fodder antibiotics species, or can
Suitable Antibiotics are selected as a control group according to required therapeutic effect, including but not limited to colistine sulfate, quinoline second
Alcohol, kitasamycin etc..
The present invention appraisal procedure in, gavage treatment diarrhoea animal gavage the time can be 2~4 days, preferably 3 days,
Animal suffer from diarrhoea as being not improved, stops gavaging at once.
In the appraisal procedure of the present invention, diarrhoea animal is the bacterial diarrhea of moderate or severe.Select moderate or severe abdomen
The influence of animal self-healing situation can preferably be excluded by rushing down animal, and the degree of moderate or severe can be according to the common animal abdomen in this area
Standards of grading are rushed down to determine.For example, the diarrhoea standards of grading of piglet can be found in：Si Mabofeng etc., " different material type creep feeds are to breast
Weaned piglets and diarrhoea, the influence of coat condition ", 2011.Bacterial diarrhea refers to by Escherichia coli, salmonella, Wei
The bacterial diarrhoea such as family name clostridium.
In the appraisal procedure of the present invention, the quantity for animal of suffering from diarrhoea typically can be more than 20, the usual more gained of quantity
Assessment result is more reliable, but excessive experimental animal can bring the inferior position in cost, therefore preferred amount is 20~30.
In the appraisal procedure of the present invention, it can also include continuing to gavage consolidation on the basis of diarrhoea animal rehabilitation rate is assessed
Treatment 2 days, by assess animal diarrhoea repeatedly rate with assess the additive agent for feeding replace anti-performance.Rate can be anti-repeatedly for diarrhoea
The stability that additive replaces anti-performance is mirrored, additive can be also reflected from another point of view replaces anti-performance.
The appraisal procedure of the present invention is applicable to the fields such as domestic animal, poultry feed, preferably can be nursery-stage pig feed addition
The assessment of agent.
Anti-performance combined type appraisal procedure is replaced present invention also offers a kind of additive agent for feeding, including primary dcreening operation and is commented again
Estimate two steps, wherein, the primary dcreening operation step is using the appraisal procedure described in above any one of technical scheme.
Appraisal procedure can select the evaluation profile of traditional feeding animals again, can by the combination of two kinds of evaluation profiles
The high efficiency and accuracy for resistance evaluation are further improved, especially when additive types to be assessed are various and for anti-performance
When indefinite.
The additive agent for feeding that the present invention is provided for anti-performance appraisal procedure changes traditional evaluation profile, by gavaging
Diarrhoea animal simultaneously investigates the rehabilitation situation of animal to assess the antibiotic substitution effect of additive, can significantly shorten the assessment time,
Without large-scale farming site, the cost of assessment is greatly reduced, efficiency, and assessment result and Traditional measurements side is improved
Method is close, as a result accurately, reliably.The appraisal procedure of the present invention can be used alone, and can act also as primary dcreening operation step and Traditional measurements side
Method is applied in combination, and will be a kind of new, efficient additive agent for feeding in face of in the market increasingly numerous additive agent for feeding product
For anti-performance appraisal procedure.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, the example of the present invention will be further described below
The technical scheme of property embodiment.
The joy of embodiment 1 treasured (plant extracts) gavages contrast test with super colistin (colistine sulfate)
Joy treasured is commercial diet additive (the honest Biological Co., Ltd. in Wuxi), and its main component is：Barrenwort,
(the icariin 0.08%-0.12%, Quercetin such as purslane, wolf's milk, the fruit of Cherokee rose, fruit of glossy privet>0.01%, oleanolic acid>
0.008%).At present, the precious main function as feed addictive of joy is antibacterial, anti-inflammatory, protects gastrointestinal tract mucous and adjust
Gastrointestinal function is saved, still without clearly for anti-performance report.
The present embodiment investigates the effect for gavaging treatment grice diarrhoea, and is compareed with existing feeding antibiotic, so that
Assess as the feasibility that traditional feeding antibiotic is substituted or supplemented in pig feed.
1st, subjects：
Diarrhoea piglet：In the case where pig farm is without obvious epidemic situation, using super colistin as control group, joy treasured is experiment
Group, carries out gavaging treatment to nursery house diarrhoea piglet.It is as invalid in gavaged 3 days, illustrate not suit the medicine to the illness, stop in time.
(1) the precious operational version of joy
By the precious product of 1.5g joys and 15ml warm water rush it is molten cool 5-10 minutes, remove volume after residue, dissolving and rise to
17ml, it is desirable to now with the current.Using the instrument that gavages such as disposable syringe, properly hold with the selection of pig size according to dosage is gavaged
Long-pending gavages instrument.For diarrhoea piglet, every diarrhoea pig gavages twice daily, and each 8.5ml must shake before gavaging every time
Swing, mix, joy treasured gavages total head number and reaches 20.
(2) super colistin operational version
The super colistins of 1g are mixed with 40ml physiological saline.Using the instrument that gavages such as disposable syringe, according to gavaging
Dosage gavages instrument with pig size selection appropriate volume.For diarrhoea piglet, every diarrhoea pig gavages twice, every time daily
10ml, must sway before gavaging every time, mix, and be gavaged immediately, and super colistin gavages total head number and reaches 20.
2nd, effect observation and record
Observed before gavaging every morning after piglet recovery situation of once suffering from diarrhoea, record rehabilitation pig's head number and rehabilitation repeatedly
Head number, and calculate rehabilitation rate.
3rd, experiment duration (treatment+consolidation)
Every diarrhoea piglet treatment is gavaged 3 days, is gavaged and is continued to gavage consolidation in 2 days after taking effect, whole viewing test object is extensive
Whether there is situation repeatedly after multiple；As gavage 3 days it is still invalid if stop at once, eliminate, concomitant medicament treatment.
4th, result of the test
Result of the test is respectively as shown in table 1, table 2：Joy treasured group piglet gavage treatment still have 8 grice diarrhoeas within the 3rd day
(eliminating out experiment), proceeds without situation repeatedly after the after treatment of 2 days, rehabilitation rate is for 12 piglets of rehabilitation
60%；And super colistin group piglet is gavaging the 3rd day after treating, 14 diarrhoea piglet rehabilitations are still suffered from diarrhoea for 6 in addition
(eliminating out experiment), does not occur situation repeatedly in follow-up after treatment, and rehabilitation rate is 70%.
The test effect of the joy of table 1 treasured group piglet
The test effect of the super colistin group piglet of table 2
Consolidated statement 1, the result of table 2 understand that super colistin effect in terms for the treatment of piglet anti diar rhea is gavaged is good, instant effect,
Effect is more slightly worse than super colistin in terms of joy treasured treatment diarrhoea, but also has obvious therapeutic effect, and is preventing diarrhoea
Aspect and super colistin effect quite, illustrate that joy treasured has preferable antibiotic substitution effect repeatedly, can be used as feeding anti-
The substitute or complementary goods of raw element.
5th, compliance test result
It is precious as feed addictive progress Animal experiment using joy, that is, pass through traditional feeding experiment and verify joy
It is alternative whether treasured possesses antibiotic.In on April 10,27 days to 2017 March in 2017 Wuhan Tian kind grow up pig farm carry out.
Experiment selection miscellaneous is protected with group, body weight is write instructions and transfer in 10kg or so the red long Bai Yudan genealogies of law Yorkshire binary of the genealogy of law of health
Piglet 225 is educated, 3 treatment groups, the respectively precious group of medicine group, joy, blank control group, 3 weights of each processing are randomly divided into
It is multiple, 25 piggys are each repeated, the feeding experiment of 14 days by a definite date is carried out.
The antibiotic of medicine group includes：10% super colistine sulfate, 20ppm；50% kitasamycin, 55ppm；Effectively
Composition meter.
The precious product of joy of the joy treasured group containing 1000ppm.
Testing material is responsible for producing and provided by Guangdong Jin Xinnong feed corporation,Ltds, is particulate material, the actual measurement of testing material
Value is shown in Table 3.
The measured value of the testing material of table 3
| Testing material | Moisture content (%) | Crude protein (%) | Crude ash content (%) | Calcium (%) | Total phosphorus (%) |
| Medicine group | 13.42 | 17.70 | 5.43 | 0.85 | 0.68 |
| Joy treasured group | 12.74 | 18.15 | 5.46 | 0.86 | 0.71 |
| Blank control group | 13.06 | 18.13 | 5.53 | 0.86 | 0.69 |
The management of test pig is grown up by day kind based on the actual feeding management in pig farm, assistant research fellow person personal management, and each group is in phase
Carried out under same feeding management and environmental condition, keep feed fresh, keep pig house environment comfortable, daily viewing test pig is adopted
Food, health and active situation, find to be the pig number of marking, track record date of the onset, duration, treatment side after sick pig
Elimination test column is wanted in case, rehabilitation situation, serious morbidity, and weighs the body weight of sick pig, carries out the record of date and body weight.
Determine tests below index：
(1) production performance：Daily ingestion amount, daily gain, feedstuff-meat ratio.
(2) Scours index：It was found that being the pig number of marking after diarrhoea sick pig, keep track of date of the onset, duration, control
Treatment scheme, rehabilitation situation；Elimination test column is wanted in serious morbidity, and weighs the body weight of sick pig, carries out the record of date and body weight.Root
Calculated according to appraisal result per nest Scours index, its calculation formula is：Per nest Scours index=observed and recorded fraction summation/(observation
Total number of days * is per litter size).
The production performance of experiment pig is shown in Table 4.
Table 4 tests pig production performance grade form
| Processing | Medicine group | Joy treasured group | Blank control group |
| Test number of days | 14 | 14 | 14 |
| Test first days of a year or a month number | 75 | 75 | 75 |
| Test initial age in days | 35 | 35 | 35 |
| First counterpoise (kg) | 10.199±1.210 | 10.250±1.245 | 10.254±1.332 |
| Last head number | 74 | 75 | 74 |
| Last counterpoise (kg) | 15.650±0.998 | 15.687±1.747 | 15.600±2.054 |
| ADFI(g) | 575±11 | 576±24 | 570±54 |
| ADG(g) | 389±25 | 388±33 | 382±44 |
| FCR | 1.478±0.41 | 1.484±0.80 | 1.492±0.86 |
| Scours index | 0.025±0.048 | 0.028±0.023 | 0.037±0.021 |
Remarks：Indivedual pig pin pain or crippled and reject the sick pig that falls down to the ground in process of the test
As seen from the results in Table 4：
(1) in terms of average daily gain, medicine group is 575g/ heads, and joy treasured group is 576g/ heads, and blank control group is
570g/ heads, medicine group is more or less the same with the precious group of joy, and joy treasured group is than blank control group increase by 1.05%, and medicine group compares blank
Control group increase by 0.88%；(2) in terms of average daily gain, medicine group is 389g/ heads/d, and joy treasured group is 388g/ heads/d, empty
White control group is 382g/ heads/day, and medicine group is more or less the same with the precious group of joy, and joy treasured group increases by 1.57% than blank control group,
Medicine group is than blank control group increase by 1.83%；(3) in terms of feedstuff-meat ratio, medicine group is 1.478, and joy treasured group is 1.484, empty
White control group is 1.492；(4) in terms of diarrhoea, medicine group Scours index is 0.025, and joy treasured group Scours index is 0.028, empty
White control group Scours index is 0.037.Draw based on the above results：Medicine group is superior to sky with joy treasured group weaned piglets
White control group, and medicine group is suitable with joy treasured group performance, absolutely proves, show that joy treasured has under Traditional measurements method preferable
Antibiotic substitution effect, can be used as the substitute or complementary goods of feeding antibiotic.
In summary, animal gavages experiment and the assessment result of feeding experiment is basically identical, i.e. joy treasured is to grice diarrhoea
Have preferable effect, illustrate that the appraisal procedure result of the present invention is accurate, reliably, and relative to conventional method take it is short, need not
Large-scale experiment place, is a kind of quick, efficiently, inexpensive appraisal procedure.
The U.S.A of embodiment 2 helps strong (plant extracts) and gavages contrast test with super colistin
Mei You strengthens (active ingredient：1.5% sanguinarine, 0.75% Chelerythrine) it is commercially available herbal medicine additive (lake
South America is up to living resources Co., Ltd), it is the feed addictive of known alternative antibiotic, investigates young by gavaging treatment
The effect of diarrhea of pigs.
1g U.S.As are helped into strong product to mix with 50ml physiological saline, U.S.A, which helps to strengthen, is slightly soluble in water, and need to stir makes it fully dissolve.Adopt
Diarrhoea piglet is gavaged with the method for embodiment 1, and super colistin is set as a control group, every group gavages total head number and reach
To 20.
Result of the test is respectively as shown in table 5, table 6：The strong group piglets of Mei You still have 10 grice diarrhoeas on the 3rd day gavaging treatment
(eliminating out experiment), proceeds without situation repeatedly after the after treatment of 2 days, rehabilitation rate is for 10 piglets of rehabilitation
50%；And super colistin group piglet is gavaging the 3rd day after treating, 14 diarrhoea piglet rehabilitations are still suffered from diarrhoea for 6 in addition
(eliminating out experiment), in follow-up after treatment, has 2 situation repeatedly occur, rehabilitation rate is 60%, and rate is 10% repeatedly.
The U.S.A of table 5 helps the test effect of strong group piglet
The test effect of the super colistin group piglet of table 6
Consolidated statement 5, the result of table 6 understand, evaluates U.S.A by the method that gavages of the present invention and helps effect in terms of strong treatment diarrhoea
Can be suitable with super colistin, illustrate the present invention to gavage method as the antibiotic substitution effect of additive is assessed be a kind of letter
Single, the accurate, method of reliable results.
Unless limited otherwise, term used herein is the implication that those skilled in the art are generally understood that.
Embodiment described in the invention is not used to limit the scope of the invention merely for exemplary purpose,
Those skilled in the art can be made within the scope of the invention various other replacements, changes and improvements, thus, the invention is not restricted to
Above-mentioned embodiment, and be only defined by the claims.
Claims (10)
1. a kind of additive agent for feeding replace anti-performance appraisal procedure, it is characterised in that additive agent for feeding to be assessed is gavaged into abdomen
Rush down animal, by assessing animal rehabilitation rate with assess the additive agent for feeding replace anti-performance.
2. appraisal procedure according to claim 1, it is characterised in that the appraisal procedure includes：Will be to be assessed feeding
Additive gavages diarrhoea animal, assesses animal rehabilitation rate, reaches that 50% additive agent for feeding to be assessed indicated above possesses
For anti-performance.
3. appraisal procedure according to claim 1, it is characterised in that the appraisal procedure includes：Will be to be assessed feeding
Additive gavages diarrhoea animal, gavages diarrhoea animal as a control group using antibiotic, assesses animal rehabilitation rate, it is described right to reach
70% additive agent for feeding to be assessed indicated above for gavaging animal rehabilitation rate according to group possesses for anti-performance.
4. appraisal procedure according to claim 3, it is characterised in that the control group selects colistine sulfate, quinoline second
One or more in alcohol, kitasamycin.
5. the appraisal procedure according to claim any one of 1-4, it is characterised in that the treatment time gavaged is 3 days.
6. the appraisal procedure according to claim any one of 1-4, it is characterised in that the diarrhoea animal is moderate or severe
Bacterial diarrhea.
7. the appraisal procedure according to claim any one of 1-4, it is characterised in that the quantity of the diarrhoea animal is 20
More than, preferably 20~30.
8. the appraisal procedure according to claim any one of 2-4, it is characterised in that the appraisal procedure also includes：Continue
Gavage after treatment 2 days, by assess animal diarrhoea repeatedly rate with assess the additive agent for feeding replace resistance stabilizability.
9. the appraisal procedure according to claim any one of 1-4, it is characterised in that the additive agent for feeding is raised for child care pig
Feed additives.
10. a kind of additive agent for feeding replaces anti-performance combined type appraisal procedure, including primary dcreening operation and assesses two steps again, its feature
It is, the primary dcreening operation step is using the appraisal procedure described in claim any one of 1-9.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104146182A (en) * | 2014-08-28 | 2014-11-19 | 成都双胞胎饲料有限公司 | Biological feed additive for preventing and treating diarrhoea of piggies |
| CN105746921A (en) * | 2016-03-10 | 2016-07-13 | 曲靖双胞胎饲料有限公司 | Novel feed additives capable of effectively preventing and treating piglet diarrhea |
-
2017
- 2017-05-22 CN CN201710364004.1A patent/CN107167558A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104146182A (en) * | 2014-08-28 | 2014-11-19 | 成都双胞胎饲料有限公司 | Biological feed additive for preventing and treating diarrhoea of piggies |
| CN105746921A (en) * | 2016-03-10 | 2016-07-13 | 曲靖双胞胎饲料有限公司 | Novel feed additives capable of effectively preventing and treating piglet diarrhea |
Non-Patent Citations (4)
| Title |
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| 俞宁等: "枯草芽孢杆菌替代抗生素治疗仔猪腹泻试验", 《西昌学院学报》 * |
| 宋延飞等: "中草药添加剂替代抗生素在断奶仔猪中的应用研究", 《饲料工业》 * |
| 宋志刚等: "应用低聚糖类制剂—G•D 生命素治疗仔猪黄白痢的试验研究", 《饲料工业》 * |
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