CN107158067A - It is a kind of to be used to prevent and treat Chinese medicine composition of swine eperythrozoonosis and its preparation method and application - Google Patents
It is a kind of to be used to prevent and treat Chinese medicine composition of swine eperythrozoonosis and its preparation method and application Download PDFInfo
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- CN107158067A CN107158067A CN201710257575.5A CN201710257575A CN107158067A CN 107158067 A CN107158067 A CN 107158067A CN 201710257575 A CN201710257575 A CN 201710257575A CN 107158067 A CN107158067 A CN 107158067A
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
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Abstract
The invention discloses a kind of Chinese medicine composition for being used to prevent and treat swine eperythrozoonosis, the Chinese medicine composition is prepared by following raw material in parts by weight：3 parts of crotons, 2 parts of radix glycyrrhizae；And there is provided its preparation method and application.Beneficial effects of the present invention are：It is a kind of for preventing and treating Chinese medicine composition of swine eperythrozoonosis and its preparation method and application that the present invention is provided, Chinese herbal medicine is used for raw material, there is the Chinese medicine composition that progress scientific compatibility is obtained heat-clearing and damp-drying drug, desinsection to go long-pending effect, eperythrozoon can effectively be killed, therapeutic effect is good, cure rate is up to more than 60%, it is to avoid in treatment eperythrozoon suis use chemicals and arsenic preparation, it is to avoid the medicament residue problem in animal-derived food.
Description
Technical field
The present invention relates to technical field of animal remedy preparation, and in particular to a kind of Chinese medicine group for being used to prevent and treat swine eperythrozoonosis
Compound and its preparation method and application.
Background technology
Eperythrozoonosis is a kind of infectious diseases common to human beings and animals as caused by Infected with Eperythrozoon body.Eperythrozoon
It is the quasi-microorganism for parasitizing erythrocyte surface, blood plasma and marrow.Pig is generally easier to occur eperythrozoonosis, and pig is attached red thin
Cell space disease is to be attached to swine erythrocyte surface by eperythrozoon suis or be free in blood plasma a kind of caused acute, hot infection
Disease, has generation in many countries and regions and popular.The disease clinically with heating, anaemia, hemolytic jaundice, expiratory dyspnea,
Rubefaction etc. is characterized, and causes animal body immunosupress, breeding difficulty, low live birth index.Big portion at home in recent years
It is all in Outbreak to divide provinces and regions, and serious economic loss is caused to pig industry.Current swine eperythrozoonosis vaccine immunity is ground
Study carefully and not yet break through, the eperythrozoonosis specific medicament of pig is seldom.Present prophylactic treatment milk swine eperythrozoonosis is typically adopted
With terramycin, sulfanilamide (SN) and three nitrogen amidines and arsenic preparation etc., though there is certain effect, cure rate is not high, easily recurrence.Some even use arsenic
Preparation for treating, in spite of certain curative effect, but pollutes the body that poisonous arsenic residual compromises consumer in environment, and pork and is good for
Health.
The content of the invention
The purpose of the present invention is used to prevent and treat swine red cell aiming at above-mentioned defect of the prior art there is provided one kind
Chinese medicine composition of body disease and its preparation method and application.
To achieve these goals, the technical scheme that provides of the present invention is：It is a kind of to be used to prevent and treat swine eperythrozoonosis
Chinese medicine composition, the Chinese medicine composition is prepared by following raw material in parts by weight：3 parts of crotons, radix glycyrrhizae 2
Part.
Second object of the present invention there is provided a kind of above-mentioned Chinese medicine composition for being used to prevent and treat swine eperythrozoonosis
Preparation method, be will crotons and radix glycyrrhizae crush after cross No. three sieves, be mixed into powder according to proportioning.
Third object of the present invention there is provided a kind of above-mentioned Chinese medicine composition for being used to prevent and treat swine eperythrozoonosis
Preparing the application in being used to prevent and treat the medicine of swine eperythrozoonosis.
Further, above-mentioned application, the adding proportion of the Chinese medicine composition is adds 2mg per kg body weight daily, even
With 3 days.
Beneficial effects of the present invention are：A kind of Chinese medicine composition for being used to prevent and treat swine eperythrozoonosis that the present invention is provided
And its preparation method and application, use Chinese herbal medicine for raw material, carry out the obtained Chinese medicine composition of scientific compatibility have heat-clearing and damp-drying drug,
Desinsection goes the effect of product, can effectively kill eperythrozoon, therapeutic effect is good, and cure rate is up to more than 60%, it is to avoid in treatment pig
Chemicals and arsenic preparation are used in eperythrozoon, it is to avoid the medicament residue problem in animal-derived food.
Embodiment
Embodiment 1：
Treat artificial challenge's eperythrozoon mouse experiment：
1 test material and method：
1.1 test material：
1.1.1 experimental animal：Blood tabletting microscopy is made through tail vein blood in Kun Ming mice, small white mouse, it is determined that without attached red
Cell body infects, body weight 18~22g, and female, male half and half, purchased from medical college of Lanzhou University.
1.1.2 test apparatus and reagent：
Light microscope（XSP-C104B）, micropipettor, asepsis injector, syringe etc., PBS, physiological saline.Investigational agent
Thing：The parts by weight of crotons 6, the parts by weight of radix glycyrrhizae 4 are crushed, and are mixed, are produced.Control drug：Three nitrogen amidines, the limited duty of Lanzhou Zheng Feng pharmacy
Ren companies produce, lot number：160412.
1.2 test method：
Artificial challenge, the pig blood sample of 3 subinfection eperythrozoons will be washed through PBS, with isometric sterile saline
Dilution is used as infection sample.0.5 ml positive blood samples are inoculated with to small white mouse by the way that inocalation method is injected intraperitoneally.After five days, through tail
Venous blood collection, plus physiological saline are put on slide, and lid amplifies 1 000 times of observations, in mirror after dripping cedar oil with cover glass with oil mirror
Under see there is barbed red blood cell (having title dentation), or adhere to some semicircle or circular attachments around red blood cell and (have title mace
Shape, lemon-like etc.), red blood cell simultaneously has micro, slight vibration, and red blood cell shrinkage, deformation etc., or see in blood plasma have it is circular or
The situation of unsetting nomadic objects not of uniform size, that is, think to infect successfully.
Experiment packet, will infect successful small white mouse and is randomly divided into 5 groups, every group 10.The mouse that medicine is added after crushing
In grain, plus particle is made in suitable amount of adhesive, and the mouse grain of medicine addition 1 ‰, 2 ‰, 4 ‰ is respectively prepared；1 group is medicine low dosage
Treatment group, the mouse grain 4g/ of feeding medicine addition 1 ‰ is only；2 groups are medicine middle dosage treatment group, feeding medicine addition 2 ‰
Mouse grain 4g/ is only；3 groups are medicine high-dose therapy group, and the mouse grain 4g/ of feeding medicine addition 4 ‰ is only；4 groups are that three nitrogen amidines are treated
Group, is injected intraperitoneally by daily 5.4mg/kg body weight；5 groups of feedings without medicine mouse grain, once every three days.
2 index of assessment of curative effect：
The change of infection rate:Observation small white mouse before experiment, 20 visuals field of blood microscopy record red blood cell number, obtain red blood cell
Infection rate is (infection eperythrozoon red blood cell number/red blood cell number) × 100%, after medication every group regularly carry out daily it is red thin
The measure of born of the same parents' infection rate, METHOD FOR CONTINUOUS DETERMINATION 6 days.
3 results and analysis：
Drug therapy artificial challenge's eperythrozoon suis small white mouse experimental result is shown in Table 1.
Table 1
The high, normal, basic group of small white mouse to artificial challenge of medicine has different degrees of therapeutic action as can be seen from Table 1, wherein with
High dose group is preferably, close with the nitrogen amidine of control drug three, but the speed that infected Erythrocytes rate declines will be slower than three Dan Mi treatment groups.
Physiological saline group small white mouse infected Erythrocytes rate is not changed, and self-healing is unable to after pointing out mice infection eperythrozoon.
Embodiment 2：
Clinical trial：
1 materials and methods：
1.1 test material：
Experimental animal chooses the ill pig (Gansu Province Dingxi suburban area plant) of 163 natural infection eperythrozoons, sick pig
Poor appetite, burnout are mainly shown as, body temperature rise is up to 40~41 DEG C, and first constipation, rear diarrhoea, anorexia is had difficulty in breathing, eye
Conjunctiva elder generation flush, rear pale, xanthochromia, the inferior skin of two ears, four limbs, abdomen has reddish violet spot.Ill pig blood enters to more than
Row microscopy, and eperythrozoon positive infection pig is chosen for experimental animal.
1.2 test method：Experiment is divided into 2 groups, i.e. three nitrogen amidine groups 80 and uses medicine group 83 of the present invention.
Three nitrogen amidine groups：Intramuscular injection, 6mg/ kg（Body weight）/ d, physiological saline solution is used in conjunction 3 days；Medicine group of the present invention
The powder of the present invention that addition is prepared by preceding method, 2mg/ kg（Body weight）/ d, is used in conjunction 3 days.
2 efficacy determinations：
Recovery from illness：Clinical symptom disappearance, pig blood red blood cell has no eperythrozoon through sessile drop method microscopy.
Take a turn for the better：Clinical symptom disappearance, pig blood red blood cell is through the visible a small amount of attached corpus hemorrhagicum of sessile drop method microscopy.
It is invalid：Clinical symptoms do not disappear, and pig blood red blood cell is through the visible attached corpus hemorrhagicum of sessile drop method microscopy.
3 results：
Through the ill pig 83 of traditional Chinese powder medicine treatment infection eperythrozoon, fully recover 51, take a turn for the better 19, invalid 13, cure
Rate is 61.4%, and effective percentage is 84.3%.Through the ill pig 80 of three nitrogen amidines treatment infection eperythrozoon, fully recover 39, improvement 21
Example, invalid 20, cure rate is 48.8%, and effective percentage is 75.0%.Chinese medicine group treats eperythrozoon suis as can be seen from the results
Sick cure rate and cure rate are above three nitrogen amidine groups.
Finally it should be noted that：The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention,
Although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, it still may be used
To be modified to the technical scheme described in foregoing embodiments, or equivalent substitution is carried out to which part technical characteristic.
Within the spirit and principles of the invention, any modification, equivalent substitution and improvements made etc., should be included in the present invention's
Within protection domain.
Claims (4)
1. a kind of be used to prevent and treat the Chinese medicine composition of swine eperythrozoonosis, it is characterised in that the Chinese medicine composition be by by
Prepared according to the following raw material of parts by weight meter：3 parts of crotons, 2 parts of radix glycyrrhizae.
2. a kind of preparation method for being used to prevent and treat the Chinese medicine composition of swine eperythrozoonosis according to claim 1, its
It is characterised by, is to cross No. three sieves after crushing crotons and radix glycyrrhizae, powder is mixed into according to proportioning.
3. a kind of Chinese medicine composition for being used to prevent and treat swine eperythrozoonosis according to claim 1 is being prepared for preventing and treating
Application in the medicine of swine eperythrozoonosis.
4. application according to claim 3, it is characterised in that the adding proportion of the Chinese medicine composition is daily per kg body
2mg is re-added, is used in conjunction 3 days.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710257575.5A CN107158067B (en) | 2017-04-19 | 2017-04-19 | Traditional Chinese medicine composition for preventing and treating swine eperythrozoonosis and preparation method and application thereof |
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| CN201710257575.5A CN107158067B (en) | 2017-04-19 | 2017-04-19 | Traditional Chinese medicine composition for preventing and treating swine eperythrozoonosis and preparation method and application thereof |
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| CN107158067A true CN107158067A (en) | 2017-09-15 |
| CN107158067B CN107158067B (en) | 2021-02-26 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113577167A (en) * | 2021-08-10 | 2021-11-02 | 中国农业科学院兰州畜牧与兽药研究所 | Application of daphne giraldii nitsche in preparation of medicine for treating swine eperythrozoonosis |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101224235A (en) * | 2008-02-02 | 2008-07-23 | 中国农业科学院兰州畜牧与兽药研究所 | Treating eperythrozoonosis medicine, preparing method and uses thereof |
| CN103960542A (en) * | 2014-05-20 | 2014-08-06 | 山东新希望六和集团有限公司 | Feed and traditional Chinese medicine composition for preventing and treating swine eperythrozoonosis |
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2017
- 2017-04-19 CN CN201710257575.5A patent/CN107158067B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101224235A (en) * | 2008-02-02 | 2008-07-23 | 中国农业科学院兰州畜牧与兽药研究所 | Treating eperythrozoonosis medicine, preparing method and uses thereof |
| CN103960542A (en) * | 2014-05-20 | 2014-08-06 | 山东新希望六和集团有限公司 | Feed and traditional Chinese medicine composition for preventing and treating swine eperythrozoonosis |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113577167A (en) * | 2021-08-10 | 2021-11-02 | 中国农业科学院兰州畜牧与兽药研究所 | Application of daphne giraldii nitsche in preparation of medicine for treating swine eperythrozoonosis |
| CN113577167B (en) * | 2021-08-10 | 2022-07-01 | 中国农业科学院兰州畜牧与兽药研究所 | Application of daphne giraldii nitsche in preparation of medicine for treating eperythrozoonosis of pigs |
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