CN107115376B - 毛果鱼藤石油醚提取物的抗氧化应用 - Google Patents

毛果鱼藤石油醚提取物的抗氧化应用 Download PDF

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CN107115376B
CN107115376B CN201710529968.7A CN201710529968A CN107115376B CN 107115376 B CN107115376 B CN 107115376B CN 201710529968 A CN201710529968 A CN 201710529968A CN 107115376 B CN107115376 B CN 107115376B
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petroleum ether
derris elliptica
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杨立芳
邓丽姚
姜明国
吴家法
吴霜
石松标
郭洋洋
姜昱
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Guangxi University for Nationalities
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Abstract

本发明公开了毛果鱼藤石油醚提取物具有抗氧化作用，特别针对II型糖尿病的氧化应激有良好效果。检测结果显示，毛果鱼藤提取物对糖尿病模型小鼠的血清氧化应激性指标SOD、GSH‑PX显著性增加，亦显著性降低了MDA水平。实验研究表明，本发明毛果鱼藤石油醚提取物对II型糖尿病小鼠具有抗氧化的作用。因此，本发明为深入研究与开发壮药毛果鱼藤提供了理论依据，以期为临床提供一种新的通过抗氧化应激作用而治疗糖尿病的现代中药制剂。

Description

毛果鱼藤石油醚提取物的抗氧化应用
技术领域
本发明属于壮药毛果鱼藤技术领域，尤其涉及一种毛果鱼藤石油醚提取物的抗氧化应用。
背景技术
糖尿病与遗传、环境、胰岛素分泌缺陷等因素有关，从而引起相关代谢的紊乱、细胞凋亡，已经严重的威胁到了人类的健康。研究证明氧化应激性是诱导糖尿病的途径之一。
毛果鱼藤(Derris Eriocarpa How)为豆科鱼藤属植物，其又名土甘草，主要分布在广东、广西等地区。攀援状灌木，木质大藤本。茎棕黄色，有多数黄色瘤状突起物，断面淡黄色，纤维状。羽状复叶互生，小叶对生。花小，粉红色，呈腋生总状花序。小枝被锈色微柔毛，花期为6-7月，果期为9月至翌年1月。毛果鱼藤常被用于治疗膀胱炎、肾炎、尿道炎、咳嗽等，具有利尿除湿、镇咳化痰的功效。
发明内容
本发明要解决的技术问题是提供一种毛果鱼藤石油醚提取物的抗氧化应用，为临床提供一种新的具有良好疗效的抗氧化中药。
为解决上述技术问题，本发明采用以下技术方案：
毛果鱼藤石油醚提取物在制备抗氧化药物中的应用。
抗氧化药物为抗糖尿病氧化应激性药物。
糖尿病为II型糖尿病。
毛果鱼藤石油醚提取物来自毛果鱼藤乙醇提取物的石油醚提取部位。
毛果鱼藤石油醚提取物按以下操作制备：将干燥毛果鱼藤藤茎切片为0.5-1cm，经中药粉碎机粉碎成粗粉，过30目筛得毛果鱼藤药粉；称取毛果鱼藤药粉，用体积浓度95％的乙醇浸泡药粉24h后，加热回流提取2次，每次1h，合并乙醇提取液，浓缩，挥尽乙醇；将毛果鱼藤乙醇提取物用水混悬，加入石油醚萃取3次，合并萃取液，浓缩，最终得到毛果鱼藤石油醚提取物浸膏。
为充分利用广西、云南特有的壮药毛果鱼藤，发明人建立了一种毛果鱼藤石油醚提取物(Petroleum Ether Extract of Derris eriocarpa How.，PEEDEH)的制备方法。据此，发明人进一步研究，发现了毛果鱼藤石油醚提取物具有抗氧化作用，特别针对II型糖尿病的氧化应激有良好效果。检测结果显示，毛果鱼藤提取物对糖尿病模型小鼠的血清氧化应激性指标SOD(超氧化物歧化酶)、GSH-PX(谷胱甘肽过氧化物酶)显著性增加，亦显著性降低了MDA(丙二醛)水平。实验研究表明，本发明毛果鱼藤石油醚提取物对II型糖尿病小鼠具有抗氧化的作用。因此，本发明为深入研究与开发壮药毛果鱼藤提供了理论依据，以期为临床提供一种新的通过抗氧化应激作用而治疗糖尿病的现代中药制剂。
具体实施方式
一、毛果鱼藤石油醚提取物的制备
将干燥毛果鱼藤藤茎切成0.5-1cm切片，经中药粉碎机粉碎成粗粉，过30目筛得毛果鱼藤药粉；称取毛果鱼藤药粉1kg，用体积浓度95％的乙醇浸泡药粉24h后，加热回流提取2次，每次1h，合并乙醇提取液，浓缩，挥尽乙醇，得到毛果鱼藤乙醇提取物(92.5g)；将毛果鱼藤乙醇提取物用适量水混悬，加入500mL石油醚萃取，萃取3次，合并萃取液，浓缩，最终得到深绿色的毛果鱼藤石油醚提取物浸膏20g。
二、II型糖尿病小鼠模型的建立。
将实验小鼠体质量为18-22g的SPF级雄性KM小鼠适应性喂养3d，饲养温度为(25±1)℃、相对湿度为(60±5)％、12h的光照昼夜循环。实验前实验动物自由饮水但禁食12h，动物尾巴静脉注射STZ(120mg/kg)，辅以高糖高脂饲料(实验基础饲料60％、蔗糖20％、猪油10％、市售胆固醇1％、蛋黄9％)饲养。3d后，实验小鼠经尾静脉取血，记录小鼠FBG，FBG≥11.1mmol/L，则II型糖尿病小鼠造模成功，成功后使用基础饲料。随机将上述小鼠分毛果鱼藤石油醚部位浸膏高(800mg/kg)、中(400mg/kg)、低(200mg/kg)剂量组、二甲双胍(320mg/kg)阳性对照组、模型组及正常对照组，每组10只。每天相同的时间灌胃给药1次，正常对照组和模型组小鼠则灌胃双蒸水，上述实验动物均按照20ml/kg的体积进行灌胃。
三、实验氧化应激性指标的测定
实验步骤
灌胃给药21d，小鼠经眼球取血，将血液离心10min(4℃，4000r/min)。收集血清，使用SOD、MDA、GSH-Px试剂盒在对应波长为450nm、532nm、412nm处测定吸光度值，计算实验动物血清氧化应激性水平SOD、MDA、GSH-Px指标。
实验结果
如表1所示，毛果鱼藤石油醚浸膏提取部位高剂量及中剂量使糖尿病模型小鼠对比STZ模型组中SOD，GSH-PX含量显著性的升高(P＜0.05)，MDA含量显著性降低。由于氧化应激性是糖尿病诱发的途径之一，降低糖尿病小鼠氧化应激性作用可改善糖尿病病症。因此，壮药毛果鱼藤提取物可显著性的降低糖尿病模型小鼠的氧化应激性。
表1 毛果鱼藤石油醚提取物对II型糖尿病小鼠血清氧化应激性指标的影响结果(x±s，n＝10)
Figure BDA0001338312620000031

Claims (2)

1.毛果鱼藤石油醚提取物在制备抗糖尿病氧化应激性药物中的应用，其特征在于所述毛果鱼藤石油醚提取物按以下操作制备：将干燥毛果鱼藤藤茎切片为0.5-1cm，经中药粉碎机粉碎成粗粉，过30目筛得毛果鱼藤药粉；称取毛果鱼藤药粉，用体积浓度95％的乙醇浸泡药粉24h后，加热回流提取2次，每次1h，合并乙醇提取液，浓缩，挥尽乙醇；将毛果鱼藤乙醇提取物用水混悬，加入石油醚萃取3次，合并萃取液，浓缩，最终得到毛果鱼藤石油醚提取物浸膏；所述糖尿病为II型糖尿病。
2.根据权利要求1所述的应用，其特征在于：所述毛果鱼藤石油醚提取物来自毛果鱼藤乙醇提取物的石油醚提取部位。
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