CN107114620B - Predatory mite culture base material and using method thereof - Google Patents
Predatory mite culture base material and using method thereof Download PDFInfo
- Publication number
- CN107114620B CN107114620B CN201710439340.8A CN201710439340A CN107114620B CN 107114620 B CN107114620 B CN 107114620B CN 201710439340 A CN201710439340 A CN 201710439340A CN 107114620 B CN107114620 B CN 107114620B
- Authority
- CN
- China
- Prior art keywords
- powder
- greenhouse
- predatory
- base material
- mites
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000463 material Substances 0.000 title claims abstract description 57
- 238000000034 method Methods 0.000 title claims description 23
- 241000238876 Acari Species 0.000 claims abstract description 104
- 239000000843 powder Substances 0.000 claims abstract description 48
- 238000009395 breeding Methods 0.000 claims abstract description 25
- 230000001488 breeding effect Effects 0.000 claims abstract description 22
- ZSBXGIUJOOQZMP-UHFFFAOYSA-N Isomatrine Natural products C1CCC2CN3C(=O)CCCC3C3C2N1CCC3 ZSBXGIUJOOQZMP-UHFFFAOYSA-N 0.000 claims abstract description 21
- ZSBXGIUJOOQZMP-JLNYLFASSA-N Matrine Chemical compound C1CC[C@H]2CN3C(=O)CCC[C@@H]3[C@@H]3[C@H]2N1CCC3 ZSBXGIUJOOQZMP-JLNYLFASSA-N 0.000 claims abstract description 21
- 229930014456 matrine Natural products 0.000 claims abstract description 21
- 239000008267 milk Substances 0.000 claims abstract description 21
- 210000004080 milk Anatomy 0.000 claims abstract description 21
- 235000013336 milk Nutrition 0.000 claims abstract description 21
- 229940029982 garlic powder Drugs 0.000 claims abstract description 19
- 241001435059 Artemisia argyi Species 0.000 claims abstract description 14
- 235000010894 Artemisia argyi Nutrition 0.000 claims abstract description 14
- 244000005700 microbiome Species 0.000 claims abstract description 11
- 150000001875 compounds Chemical class 0.000 claims abstract description 5
- 235000015099 wheat brans Nutrition 0.000 claims description 34
- 241001455175 Neoseiulus cucumeris Species 0.000 claims description 32
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 18
- 244000063299 Bacillus subtilis Species 0.000 claims description 18
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 18
- 241000223260 Trichoderma harzianum Species 0.000 claims description 18
- 241000751139 Beauveria bassiana Species 0.000 claims description 14
- 238000005070 sampling Methods 0.000 claims description 9
- 238000007689 inspection Methods 0.000 claims description 8
- 239000002131 composite material Substances 0.000 claims description 6
- 235000003261 Artemisia vulgaris Nutrition 0.000 claims description 5
- 240000006891 Artemisia vulgaris Species 0.000 claims description 5
- 230000000694 effects Effects 0.000 abstract description 33
- 241000238631 Hexapoda Species 0.000 abstract description 24
- 230000001651 autotrophic effect Effects 0.000 abstract description 13
- 241000233866 Fungi Species 0.000 abstract description 11
- 230000001965 increasing effect Effects 0.000 abstract description 11
- 230000008901 benefit Effects 0.000 abstract description 6
- 230000004083 survival effect Effects 0.000 abstract description 4
- 241000894006 Bacteria Species 0.000 description 40
- 241000607479 Yersinia pestis Species 0.000 description 28
- 244000052616 bacterial pathogen Species 0.000 description 12
- 230000009286 beneficial effect Effects 0.000 description 11
- 230000000844 anti-bacterial effect Effects 0.000 description 10
- 241000220223 Fragaria Species 0.000 description 7
- 239000000575 pesticide Substances 0.000 description 7
- 244000062804 prey Species 0.000 description 7
- 230000001954 sterilising effect Effects 0.000 description 7
- 238000004659 sterilization and disinfection Methods 0.000 description 7
- 235000016623 Fragaria vesca Nutrition 0.000 description 6
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 6
- 230000002401 inhibitory effect Effects 0.000 description 6
- 230000005764 inhibitory process Effects 0.000 description 6
- 230000000749 insecticidal effect Effects 0.000 description 6
- 235000016709 nutrition Nutrition 0.000 description 6
- 230000002829 reductive effect Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 230000002265 prevention Effects 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 241001414989 Thysanoptera Species 0.000 description 4
- 230000003385 bacteriostatic effect Effects 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 230000002708 enhancing effect Effects 0.000 description 4
- 239000003205 fragrance Substances 0.000 description 4
- 230000036039 immunity Effects 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 230000035764 nutrition Effects 0.000 description 4
- 241001658758 Neoseiulus Species 0.000 description 3
- 241001454295 Tetranychidae Species 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 241000488583 Panonychus ulmi Species 0.000 description 2
- 241000179203 Phytoseiulus persimilis Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 230000007969 cellular immunity Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000004727 humoral immunity Effects 0.000 description 2
- 230000002147 killing effect Effects 0.000 description 2
- 231100000053 low toxicity Toxicity 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000000384 rearing effect Effects 0.000 description 2
- 230000003248 secreting effect Effects 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 241000406588 Amblyseius Species 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 241000258937 Hemiptera Species 0.000 description 1
- 241001206567 Neoseiulus barkeri Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000488585 Panonychus Species 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000002226 simultaneous effect Effects 0.000 description 1
- 235000021012 strawberries Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a predatory mite culture base material which comprises the following components in parts by weight: 77-83% of pollen, 5-15% of milk powder, 3-7% of compound microorganism, 2-4% of garlic powder, 0.5-1.5% of artemisia argyi powder and 0.5-1.5% of matrine. After the breeding base material is mixed with the conventional feed to feed the predatory mites, the breeding base material has the advantages of improving the survival rate, the growth and development rate and the reproduction rate of the predatory mites, increasing the population growth speed, increasing the field activity capability and the prey search capability of the predatory mites, preventing the breeding of the predatory mites from polluting sundry fungus and sundry insects under natural conditions, and realizing autotrophic self-breeding under natural conditions after the breeding.
Description
Technical Field
The invention relates to a technology for artificially feeding mites in the agricultural field, in particular to a predatory mite culture base material and a using method thereof.
Background
The strawberry planting area in China is rapidly increased in recent years, but due to successive year planting, the damage of strawberry insect pests is increased year by year, and the normal growth of strawberries is seriously influenced by red spider, thrips, whiteflies and other pests. At present, part of pests generate certain resistance to chemical pesticides, and pesticide residues of products bring potential safety hazards to consumers, so that the natural enemy predatory mites are widely used for preventing and treating strawberry pests in a large area, and the method is particularly important for the development of food safety and the strawberry industry. Predatory mites are main natural enemies of pests such as pest mites and thrips on various agricultural and forestry crops, and have strong control effect on the harm of small pests. Predatory mites have been used for decades at home and abroad to control pests and harmful mites. At present, various predatory mites are commercially produced, such as Phytoseiulus persimilis (Phytoseiulus persimilis), Neoseiulus persimilis (Neoseiulus barkeri), Neoseiulus persimilis (Neoseiulus cuumeris), Amblyseius cucumeris (Amblyseius cuumeris) and the like, and the commercial predatory mites are widely used for controlling pests such as pest mites in fields, greenhouses and orchards, thrips and the like, such as 70-75% of greenhouse flowers and vegetables in British, Finland, Sweden and other countries, such as the British, the vegetable mites mainly use the predatory mites to control red spiders, thrips and tarsal mites; the newly small cucumeris amblyseius cucumeris and artificial propagation technology are introduced abroad in our country from the end of the last 90 years, predatory mites are widely used for preventing and controlling pest mites and pests of agricultural and forestry crops, the dosage of chemical pesticides is effectively reduced, if the predatory mites are applied to oranges to prevent and control citrus panonychus, the chemical pesticide dosage can be reduced by more than 40%, and the predatory mites become an important substitute for chemical control of pest mites.
The feed for artificially feeding predatory mites in large quantities mainly comprises pollen, natural preys (such as tetranychidae), substitute preys (such as tetranychidae, sweet fruit mites and the like), artificial feed and the like, wherein the method for commercially breeding the predatory mites is commonly adopted due to the advantages of wide sources of the substitute preys, such as tetranychidae, low feeding cost, easy acquisition and the like. The main principle of artificially feeding predatory mites in large quantities by using substitute preys (flour mites, sweet fruit mites and the like) is to breed a large quantity of substitute preys (flour mites, sweet fruit mites and the like) by using feeds such as wheat bran, rice bran and the like and then substitute the predatory mites required by breeding the preys. The traditional method for cultivating predatory mites has high requirement on cultivation conditions and is complex to operate, so that the cost is high (including house renting, depreciation, labor cost, enterprise management cost and the like), and the predatory mites are sold after cultivation, so that the transportation cost and the sale cost are further increased, the actual use cost of farmers is high, and the popularization is delayed in recent years. The facility for farmers to breed predatory mites under natural conditions is simple and low in cost, but is easily interfered by external environment, easily pollutes infectious microbes and other insects, and simultaneously the breeding quantity of the predatory mites is limited and the predatory activity is reduced. Therefore, the invention needs to invent a predatory mite culture base material which can realize autotrophic and autotrophic propagation under natural conditions after farmer introduction, and reduce the cost of strawberry pest prevention and control. Meanwhile, the invention also needs to invent a use method for applying the breeding base material to predatory mite breeding, which is simple and convenient to operate and easy to popularize.
Disclosure of Invention
By selecting reasonable component combinations and the proportion of the components, the invention provides a predatory mite culture base material which can realize autotrophic and autotrophic propagation under natural conditions after farmer introduction. Meanwhile, the application method of the breeding base material for feeding predatory mites is simple and convenient to operate and easy to popularize.
In order to achieve the purpose, the invention provides a predatory mite breeding base material which comprises the following components in parts by weight: 77-83% of pollen, 5-15% of milk powder, 3-7% of compound microorganism, 2-4% of garlic powder, 0.5-1.5% of artemisia argyi powder and 0.5-1.5% of matrine. The conventional feed such as bran, wheat bran and the like is singly used, because the conventional feed has no insect control and bacteria inhibition effects, cannot increase the activity of predatory mites, is easy to pollute mixed bacteria and other insects, so that the breeding amount of the predatory mites after eating is limited, the predatory activity is reduced, and the autotrophic self-breeding cannot be realized under natural conditions, therefore, the invention adds the breeding base material which has certain insect control and bacteria inhibition effects and can increase the activity of the predatory mites to nurture the predatory mites in the formula of the conventional feed. The existence of the pollen can improve the survival rate, the growth and development rate and the reproduction rate of the predatory mites, and has obvious effects on enhancing the planting capability of the predatory mites and controlling the effect of pests; the milk powder contains rich protein, and can improve the amino acid content in prey feed, thereby improving the development and reproduction capability of predatory mites and increasing the population growth speed; the composite microorganism controls the propagation of harmful bacteria in a mode that beneficial bacteria occupy the place preferentially, thereby ensuring that the nutrient components in the feed mixed with the culture base material are not damaged by the harmful bacteria, ensuring that predatory mites fed by the feed mixed with the culture base material are not influenced by the harmful bacteria to grow and develop, promoting the diffusion of the predatory mites in the field, increasing the activity of the predatory mites in the field and the search capability of the prey, and accelerating the prevention and control; the garlic powder is a natural plant broad-spectrum antibiotic, can effectively inhibit the growth and reproduction of harmful fungi, has obvious control effect on mixed fungi of the culture base material, and does not influence the growth and reproduction activities of beneficial fungi; the mugwort powder has a special fragrance, the fragrance has the efficacy of expelling insects and the effects of resisting bacteria and viruses, predatory mites cultured under natural conditions are not easy to pollute mixed bacteria and mixed insects, and autotrophic self-propagation can be realized under natural conditions after introduction; matrine is a plant source pesticide, can be rapidly decomposed in nature, is a low-toxicity, low-residue and environment-friendly pesticide, has insecticidal and bactericidal activities, has good control effects on mixed bacteria and mixed insects, is not easy to pollute the mixed bacteria and the mixed insects when being cultivated and predatory mites under natural conditions, and can realize autotrophic self-reproduction under natural conditions after being introduced; it is important to note that the above-described benefits are not solely directed to the claims.
Further, the composite microorganism comprises 1.5-2.5% of beauveria bassiana, 0.5-1.5% of trichoderma harzianum, 0.5-1.5% of bacillus subtilis and 0.5-1.5% of bacillus amyloliquefaciens according to the weight ratio. The molecular spores generated by the beauveria bassiana penetrate the body wall of the pests through the action of epidermal degradation enzyme, so that the nutrition and the moisture in the pests are absorbed, the pests are further killed, and the insecticidal effect is achieved; trichoderma harzianum dissolves cell walls of pathogenic bacteria by secreting extracellular enzymes, penetrates hypha to absorb nutrition, further kills the pathogenic bacteria and plays a role in sterilization; the bacillus subtilis generates active substances for inhibiting pathogenic bacteria in the growth process, enhances the cell immunity and humoral immunity functions of predatory mites, improves the immunity of groups, and plays roles in inhibiting bacteria and enhancing immunity; the bacillus amyloliquefaciens secretes antibacterial substances to inhibit the growth of pathogenic bacteria, and can generate extracellular hydrolase which plays an important role in inhibiting the pathogenic bacteria and has an antibacterial effect besides generating antibiotics; the predatory mites of the culture medium are carried with beneficial bacteria such as white muscardine fungi, trichoderma harzianum, bacillus subtilis, bacillus amyloliquefaciens and the like, and have the functions of killing insects and resisting bacteria, and the two functions simultaneously ensure that the nutrient components in the feed can be completely absorbed by the predatory mites, protect the predatory mites from being influenced by harmful bacteria to normally grow and develop, prevent the damage of pests to the growth of the predatory mites, increase the activity of the predatory mites in the field and the search capability of the predatory mites on prey and accelerate the prevention and control. It is important to note that the above-described benefits are not solely directed to the claims.
Further, the culture base material comprises the following components in parts by weight: 80% of pollen, 10% of milk powder, 5% of compound microorganism, 3% of garlic powder, 1% of mugwort powder and 1% of matrine.
Further, the culture base material comprises the following components in parts by weight: 80% of pollen, 10% of milk powder, 2% of beauveria bassiana, 1% of trichoderma harzianum, 1% of bacillus subtilis, 1% of bacillus amyloliquefaciens, 3% of garlic powder, 1% of artemisia argyi powder and 1% of matrine. The mixture of the pollen and the milk powder with the weight ratio of 90 percent completely meets the growth nutritional requirements of the predatory mites, improves the development and reproduction capability of the predatory mites, increases the population growth speed, and ensures that the predatory mites eating the mixed feed have quick development, easy reproduction and activity; particularly, the muscardine, the trichoderma harzianum, the bacillus subtilis, the bacillus amyloliquefaciens, the garlic powder, the artemisia argyi powder and the matrine are proportioned according to the weight ratio of 2:1:1:1:3:1:1, so that the insecticidal, bactericidal and bacteriostatic effects of the feed are balanced, the components with bacteriostatic action are slightly heavier in proportioning from the aspect of proportioning, only the growth of harmful bacteria is fundamentally inhibited, the microecological balance in the feed base is balanced, beneficial bacteria are adopted to inhibit pathogenic bacteria (harmful bacteria), no new harmful bacteria can be generated in the feed base by 'beneficial bacteria inhibition', and the environment in the feed base is maintained by matching the components with the bactericidal action, so that the predatory mites after eating the feed are fast in development, easy to reproduce and active. It is important to note that the above-described benefits are not solely directed to the claims.
The invention also provides a use method of the predatory mite culture base material.
The use method of the predatory mite culture base material comprises the following steps:
a. mixing pollen, milk powder, composite microorganism, Bulbus Allii powder, folium Artemisiae Argyi powder, and matrine uniformly to form culture base material;
b. according to the weight ratio, 95% of wheat bran is placed in a steamer to be steamed for 10 minutes, and after the wheat bran is cooled, the wheat bran and 5% of culture base materials are uniformly mixed;
c. the powder mites were inoculated at 1000 heads/ml and cultured under the following conditions: the temperature in the greenhouse is 15-30 ℃ in the daytime, the temperature at night is not less than 8 ℃, and the humidity in the greenhouse is 65-90%;
d. after 1 week, 10 predatory mite seeds/ml are put into the greenhouse, and the cultivation is continued under the following conditions: the temperature in the greenhouse is 15-30 ℃ in the daytime, the temperature at night is not less than 8 ℃, and the humidity in the greenhouse is 65-90%;
e. sampling inspection is carried out after 3 weeks, and the culture is qualified when the number of predatory mites reaches more than 100 heads/ml;
f. the prey mites which are qualified in culture are applied on the spot and applied once every 10 days.
Further, a use method of the predatory mite culture base material comprises the following steps:
a. mixing 80% of pollen, 10% of milk powder, 2% of beauveria bassiana, 1% of trichoderma harzianum, 1% of bacillus subtilis, 1% of bacillus amyloliquefaciens, 3% of garlic powder, 1% of artemisia argyi powder and 1% of matrine uniformly according to the weight ratio to form a culture base material;
b. according to the weight ratio, 95% of wheat bran is placed in a steamer to be steamed for 10 minutes, and after the wheat bran is cooled, the wheat bran and 5% of culture base materials are uniformly mixed;
c. the powder mites were inoculated at 1000 heads/ml and cultured under the following conditions: the temperature in the greenhouse is 23 ℃ in the daytime, the temperature at night is 11 ℃, and the humidity in the greenhouse is 78%;
d. after 1 week, 10 predatory mite seeds/ml are put into the greenhouse, and the cultivation is continued under the following conditions: the temperature in the greenhouse is 23 ℃ in the daytime, the temperature at night is 11 ℃, and the humidity in the greenhouse is 78%;
e. sampling inspection is carried out after 3 weeks, and the culture is qualified when the number of predatory mites reaches more than 100 heads/ml;
f. the prey mites which are qualified in culture are applied on the spot and applied once every 10 days.
By selecting reasonable component combination and the proportion among the components, the invention has the following beneficial effects:
1. the conventional feed such as bran, wheat bran and the like is singly used, because the conventional feed has no insect control and bacteria inhibition effects, cannot increase the activity of predatory mites, is easy to pollute mixed bacteria and other insects, so that the breeding amount of the predatory mites after eating is limited, the predatory activity is reduced, and the autotrophic self-breeding cannot be realized under natural conditions, therefore, the invention adds the breeding base material which has certain insect control and bacteria inhibition effects and can increase the activity of the predatory mites to nurture the predatory mites in the formula of the conventional feed. The existence of the pollen can improve the survival rate, the growth and development rate and the reproduction rate of the predatory mites, and has obvious effects on enhancing the planting capability of the predatory mites and controlling the effect of pests; the milk powder contains rich protein, and can improve the amino acid content in prey feed, thereby improving the development and reproduction capability of predatory mites and increasing the population growth speed; the composite microorganism controls the propagation of harmful bacteria in a mode that beneficial bacteria occupy the place preferentially, thereby ensuring that the nutrient components in the feed mixed with the culture base material are not damaged by the harmful bacteria, ensuring that predatory mites fed by the feed mixed with the culture base material are not influenced by the harmful bacteria to grow and develop, promoting the diffusion of the predatory mites in the field, increasing the activity of the predatory mites in the field and the search capability of the prey, and accelerating the prevention and control; the garlic powder is a natural plant broad-spectrum antibiotic, can effectively inhibit the growth and reproduction of harmful fungi, has obvious control effect on mixed fungi of the culture base material, and does not influence the growth and reproduction activities of beneficial fungi; the mugwort powder has a special fragrance, the fragrance has the efficacy of expelling insects and the effects of resisting bacteria and viruses, predatory mites cultured under natural conditions are not easy to pollute mixed bacteria and mixed insects, and autotrophic self-propagation can be realized under natural conditions after introduction; matrine is a plant source pesticide, can be rapidly decomposed in nature, is a low-toxicity, low-residue and environment-friendly pesticide, has insecticidal and bactericidal activities, has good control effects on mixed bacteria and mixed insects, is not easy to pollute the mixed bacteria and the mixed insects when being cultivated and predatory mites under natural conditions, and can realize autotrophic self-reproduction under natural conditions after being introduced; therefore, after the cultivation base material is mixed with the conventional feed to feed the predatory mites, the cultivation base material has the advantages of improving the survival rate, the growth and development rate and the reproduction rate of the predatory mites, increasing the population growth speed, increasing the activity capability of the predatory mites in the field and the searching capability of the predatory mites on prey, preventing the cultivated predatory mites from polluting sundry fungi and sundry insects under natural conditions, and realizing autotrophic self-reproduction under natural conditions after introduction;
2. the molecular spores generated by the beauveria bassiana penetrate the body wall of the pests through the action of epidermal degradation enzyme, so that the nutrition and the moisture in the pests are absorbed, the pests are further killed, and the insecticidal effect is achieved; trichoderma harzianum dissolves cell walls of pathogenic bacteria by secreting extracellular enzymes, penetrates hypha to absorb nutrition, further kills the pathogenic bacteria and plays a role in sterilization; the bacillus subtilis generates active substances for inhibiting pathogenic bacteria in the growth process, enhances the cell immunity and humoral immunity functions of predatory mites, improves the immunity of groups, and plays roles in inhibiting bacteria and enhancing immunity; the bacillus amyloliquefaciens secretes antibacterial substances to inhibit the growth of pathogenic bacteria, and can generate extracellular hydrolase which plays an important role in inhibiting the pathogenic bacteria and has an antibacterial effect besides generating antibiotics; the predatory mites of the culture medium carry beneficial bacteria such as white muscardine fungi, trichoderma harzianum, bacillus subtilis, bacillus amyloliquefaciens and the like, and have the functions of killing insects and resisting bacteria, and the two aspects have the simultaneous effects, so that the nutrient components in the feed can be completely absorbed by the predatory mites, the predatory mites are protected from being influenced by harmful bacteria to normal growth and development, the damage of pests to the growth of the predatory mites is prevented, the activity capability of the predatory mites in the field and the search capability of the predatory mites on preys are improved, and the purpose of prevention and control is accelerated;
3. the mixture of the pollen and the milk powder with the weight ratio of 90 percent completely meets the growth nutritional requirements of the predatory mites, improves the development and reproduction capability of the predatory mites, increases the population growth speed, and ensures that the predatory mites eating the mixed feed have quick development, easy reproduction and activity; particularly, the muscardine fungi, the trichoderma harzianum, the bacillus subtilis, the bacillus amyloliquefaciens, the garlic powder, the artemisia argyi powder and the matrine are proportioned according to the weight ratio of 2:1:1:1:3:1:1, so that the insecticidal, bactericidal and bacteriostatic effects of the feed are balanced, the components with bacteriostatic action are slightly heavier in proportioning from the aspect of proportioning, only the growth of harmful bacteria is fundamentally inhibited, the microecological balance in the feed base is balanced, beneficial bacteria are adopted to inhibit pathogenic bacteria (harmful bacteria), no new harmful bacteria can be generated in the feed base by 'beneficial bacteria inhibition', and the environment in the feed base is maintained by matching the components with the bactericidal action, so that the predatory mites after eating the feed are fast in development, easy to reproduce and active;
4. the predatory mite culture base material is prepared by selecting reasonable component combinations and the proportion of the components, and the predatory mites are fed after being mixed with the traditional feed, and have strong predatory capability, fast development, easy propagation and strong adaptability after eating.
5. The invention also provides a use method of the predatory mite culture base material, and the method is simple and convenient to operate and easy to popularize.
The culture base material which has certain insect control and bacteriostasis functions and can increase the activity of predatory mites is added into the conventional feed formula, so that the predatory mites after farmer introduction realize autotrophic self-propagation under natural conditions, and the feed has very important significance for large-area popularization of biological control technology while the cost of preventing and controlling the strawberry insects is reduced.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A predatory mite culture base material comprises the following components in parts by weight: 77% of pollen, 5% of milk powder, 1.5% of beauveria bassiana, 0.5% of trichoderma harzianum, 0.5% of bacillus subtilis, 0.5% of bacillus amyloliquefaciens, 2% of garlic powder, 0.5% of artemisia argyi powder and 0.5% of matrine.
a. Mixing 77% of pollen, 5% of milk powder, 1.5% of beauveria bassiana, 0.5% of trichoderma harzianum, 0.5% of bacillus subtilis, 0.5% of bacillus amyloliquefaciens, 2% of garlic powder, 0.5% of artemisia argyi powder and 0.5% of matrine uniformly according to the weight ratio to form a culture base material;
b. according to the weight ratio, 95% of wheat bran is placed in a steamer to be steamed for 10 minutes, after the wheat bran is cooled, sterilization measures are adopted to sterilize, and the wheat bran and 5% of culture base materials are uniformly mixed, wherein the weight of 95% of the wheat bran is 9.5kg, and the weight of 5% of the culture base materials is 0.5 kg;
c. the method comprises the following steps of inoculating 1000 heads/ml of aleyrotes tetranychii in a greenhouse, inoculating 40000 heads in total, and culturing under the following conditions: the temperature in the greenhouse is 15 ℃ in the daytime, the temperature at night is 8 ℃, and the humidity in the greenhouse is 65%;
d. after 1 week, 10 amblyseius cucumeris seeds/ml, 1200 amblyseius cucumeris seeds are put into the greenhouse, and the cultivation is carried out under the following conditions: the temperature in the greenhouse is 15 ℃ in the daytime, the temperature at night is 8 ℃, and the humidity in the greenhouse is 65%;
e. sampling inspection is carried out after 3 weeks, and the culture is qualified when the number of the amblyseius cucumeris reaches more than 100 heads/ml;
f. and (4) applying the cultured amblyseius cucumeris on the spot, and applying the amblyseius cucumeris once every 10 days.
Example 2
A predatory mite culture base material comprises the following components in parts by weight: 83% of pollen, 15% of milk powder, 2.5% of beauveria bassiana, 1.5% of trichoderma harzianum, 1.5% of bacillus subtilis, 1.5% of bacillus amyloliquefaciens, 4% of garlic powder, 1.5% of artemisia argyi powder and 1.5% of matrine.
a. Mixing 83% of pollen, 15% of milk powder, 2.5% of beauveria bassiana, 1.5% of trichoderma harzianum, 1.5% of bacillus subtilis, 1.5% of bacillus amyloliquefaciens, 4% of garlic powder, 1.5% of artemisia argyi powder and 1.5% of matrine uniformly according to the weight ratio to form a culture base material;
b. according to the weight ratio, 95% of wheat bran is placed in a steamer to be steamed for 10 minutes, after the wheat bran is cooled, sterilization measures are adopted to sterilize, and the wheat bran and 5% of culture base materials are uniformly mixed, wherein the weight of 95% of the wheat bran is 9.5kg, and the weight of 5% of the culture base materials is 0.5 kg;
c. the method comprises the following steps of inoculating 1000 heads/ml of aleyrotes tetranychii in a greenhouse, inoculating 40000 heads in total, and culturing under the following conditions: the temperature in the greenhouse is 30 ℃ in the daytime, 15 ℃ at night and 90% in the greenhouse;
d. after 1 week, 10 amblyseius cucumeris seeds/ml, 1200 amblyseius cucumeris seeds are put into the greenhouse, and the cultivation is carried out under the following conditions: the temperature in the greenhouse is 30 ℃ in the daytime, 15 ℃ at night and 90% in the greenhouse;
e. sampling inspection is carried out after 3 weeks, and the culture is qualified when the number of the amblyseius cucumeris reaches more than 100 heads/ml;
f. and (4) applying the cultured amblyseius cucumeris on the spot, and applying the amblyseius cucumeris once every 10 days.
Example 3
A predatory mite culture base material comprises the following components in parts by weight: 80% of pollen, 10% of milk powder, 2% of beauveria bassiana, 1% of trichoderma harzianum, 1% of bacillus subtilis, 1% of bacillus amyloliquefaciens, 3% of garlic powder, 1% of artemisia argyi powder and 1% of matrine.
a. Uniformly mixing 80% of pollen, 10% of milk powder, 2% of beauveria bassiana, 1% of trichoderma harzianum, 1% of bacillus subtilis, 1% of bacillus amyloliquefaciens, 3% of garlic powder, 1% of artemisia argyi powder and 1% of matrine according to the weight ratio to form a culture base material;
b. according to the weight ratio, 95% of wheat bran is placed in a steamer to be steamed for 10 minutes, after the wheat bran is cooled, sterilization measures are adopted to sterilize, and the wheat bran and 5% of culture base materials are uniformly mixed, wherein the weight of 95% of the wheat bran is 9.5kg, and the weight of 5% of the culture base materials is 0.5 kg;
c. the method comprises the following steps of inoculating 1000 heads/ml of aleyrotes tetranychii in a greenhouse, inoculating 40000 heads in total, and culturing under the following conditions: the temperature in the greenhouse is 23 ℃ in the daytime, the temperature at night is 11 ℃, and the humidity in the greenhouse is 78%;
d. after 1 week, 10 amblyseius cucumeris seeds/ml, 1200 amblyseius cucumeris seeds are put into the greenhouse, and the cultivation is carried out under the following conditions: the temperature in the greenhouse is 23 ℃ in the daytime, the temperature at night is 11 ℃, and the humidity in the greenhouse is 78%;
e. sampling inspection is carried out after 3 weeks, and the culture is qualified when the number of the amblyseius cucumeris reaches more than 100 heads/ml;
f. and (4) applying the cultured amblyseius cucumeris on the spot, and applying the amblyseius cucumeris once every 10 days.
Comparative example
a. According to the weight ratio, 95% of wheat bran is placed in a steamer to be steamed for 10 minutes, after the wheat bran is cooled, sterilization measures are adopted for sterilization, and the wheat bran and 5% of sterilized wheat bran are uniformly mixed, wherein the weight of 95% of the wheat bran is 9.5kg, the weight of 5% of the sterilized wheat bran is 0.5kg, namely the weight of the sterilized wheat bran is 10kg, and no culture base material is added;
b. the method comprises the following steps of inoculating 1000 heads/ml of aleyrotes tetranychii in a greenhouse, inoculating 40000 heads in total, and culturing under the following conditions: the temperature in the greenhouse is 23 ℃ in the daytime, the temperature at night is 11 ℃, and the humidity in the greenhouse is 78%
c. After 1 week, 10 amblyseius cucumeris seeds/ml, 1200 amblyseius cucumeris seeds are put into the greenhouse, and the cultivation is carried out under the following conditions: the temperature in the greenhouse is 23 ℃ in the daytime, the temperature at night is 11 ℃, and the humidity in the greenhouse is 78%;
d. sampling inspection is carried out after 3 weeks, and the culture is qualified when the number of the amblyseius cucumeris reaches more than 100 heads/ml;
e. and (4) applying the cultured amblyseius cucumeris on the spot, and applying the amblyseius cucumeris once every 10 days.
After 20 days, the feeding effect of amblyseius cucumeris of examples 1, 2, 3 and comparative example was counted as initial density (head/g) and continued at 30, 40, 50, 60 days, with the results as shown in the following table:
examples 1, 2 and 3 and comparative example Amblyseius cucumeris number statistics (unit: head/gram)
From the above table, it can be seen that:
after the amblyseius cucumeris is bred for 30 days, 40 days, 50 days and 60 days by using the breeding base materials in the examples 1, 2 and 3, the breeding number of the amblyseius cucumeris is obviously higher than that of the amblyseius cucumeris bred by common feed (without the breeding base materials). Wherein the maximum number of the amblyseius cucumeris proliferates after 30, 40, 50, 60 days of rearing of amblyseius cucumeris using the rearing medium of example 3.
And the occurrence of field insect pests in the greenhouses using the embodiments 1, 2 and 3 and the comparative example was counted by random sampling investigation. As shown in the table below, the occurrence of pests in the greenhouse by application of Amblyseius cucumeris in examples 1, 2 and 3 was significantly better than the comparative example, and the pest amount in the greenhouse was the least in example 3.
Greenhouse insect pest statistics for Amblyseius cucumeris applied in examples 1, 2 and 3 and comparative examples
It can be seen that the addition of the breeding base of the present invention can significantly increase the predatory capacity of predatory mites.
The predatory mite culture base material is prepared by selecting reasonable component combinations and the proportion of the components, and the predatory mites are fed after being mixed with the traditional feed, and have strong predatory capability, fast development, easy propagation and strong adaptability after eating. The formula has low cost and easy formula and is suitable for self-operation of farmers.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (6)
1. A predatory mite culture base material is characterized in that: the breeding base material comprises the following components in parts by weight: 77-83% of pollen, 5-15% of milk powder, 3-7% of compound microorganism, 2-4% of garlic powder, 0.5-1.5% of mugwort powder and 0.5-1.5% of matrine,
the composite microorganism comprises 1.5-2.5% of beauveria bassiana, 0.5-1.5% of trichoderma harzianum, 0.5-1.5% of bacillus subtilis and 0.5-1.5% of bacillus amyloliquefaciens.
2. The predatory mite cultivation base of claim 1, wherein: the breeding base material comprises the following components in parts by weight: 80% of pollen, 10% of milk powder, 5% of compound microorganism, 3% of garlic powder, 1% of mugwort powder and 1% of matrine.
3. The predatory mite cultivation base of claim 1, wherein: the breeding base material comprises the following components in parts by weight: 80% of pollen, 10% of milk powder, 2% of beauveria bassiana, 1% of trichoderma harzianum, 1% of bacillus subtilis, 1% of bacillus amyloliquefaciens, 3% of garlic powder, 1% of artemisia argyi powder and 1% of matrine.
4. The method of using a predatory mite cultivation base as claimed in claim 1, wherein: the method comprises the following steps:
a. mixing pollen, milk powder, composite microorganism, Bulbus Allii powder, folium Artemisiae Argyi powder, and matrine uniformly to form culture base material;
b. according to the weight ratio, 95% of wheat bran is placed in a steamer to be steamed for 10 minutes, and after the wheat bran is cooled, the wheat bran and 5% of culture base materials are uniformly mixed;
c. the powder mites were inoculated at 1000 heads/ml and cultured under the following conditions: the temperature in the greenhouse is 15-30 ℃ in the daytime, the temperature at night is not less than 8 ℃, and the humidity in the greenhouse is 65-90%;
d. after 1 week, 10 predatory mite seeds/ml are put into the greenhouse, and the cultivation is continued under the following conditions: the temperature in the greenhouse is 15-30 ℃ in the daytime, the temperature at night is not less than 8 ℃, and the humidity in the greenhouse is 65-90%;
e. sampling inspection is carried out after 3 weeks, and the culture is qualified when the number of predatory mites reaches more than 100 heads/ml;
f. the prey mites which are qualified in culture are applied on the spot and applied once every 10 days.
5. The method of using a predatory mite cultivation base as claimed in claim 1, wherein: the method comprises the following steps:
a. mixing 80% of pollen, 10% of milk powder, 2% of beauveria bassiana, 1% of trichoderma harzianum, 1% of bacillus subtilis, 1% of bacillus amyloliquefaciens, 3% of garlic powder, 1% of artemisia argyi powder and 1% of matrine uniformly according to the weight ratio to form a culture base material;
b. according to the weight ratio, 95% of wheat bran is placed in a steamer to be steamed for 10 minutes, and after the wheat bran is cooled, the wheat bran and 5% of culture base materials are uniformly mixed;
c. the powder mites were inoculated at 1000 heads/ml and cultured under the following conditions: the temperature in the greenhouse is 23 ℃ in the daytime, the temperature at night is 11 ℃, and the humidity in the greenhouse is 78%;
d. after 1 week, 10 predatory mite seeds/ml are put into the greenhouse, and the cultivation is continued under the following conditions: the temperature in the greenhouse is 23 ℃ in the daytime, the temperature at night is 11 ℃, and the humidity in the greenhouse is 78%;
e. sampling inspection is carried out after 3 weeks, and the culture is qualified when the number of predatory mites reaches more than 100 heads/ml;
f. the prey mites which are qualified in culture are applied on the spot and applied once every 10 days.
6. The use of a predatory mite cultivation base as claimed in claim 4 or 5, wherein: the predatory mites are amblyseius cucumeris.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710439340.8A CN107114620B (en) | 2017-06-12 | 2017-06-12 | Predatory mite culture base material and using method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710439340.8A CN107114620B (en) | 2017-06-12 | 2017-06-12 | Predatory mite culture base material and using method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107114620A CN107114620A (en) | 2017-09-01 |
| CN107114620B true CN107114620B (en) | 2020-10-02 |
Family
ID=59729187
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710439340.8A Expired - Fee Related CN107114620B (en) | 2017-06-12 | 2017-06-12 | Predatory mite culture base material and using method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107114620B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE1026685B1 (en) * | 2018-10-05 | 2020-05-07 | Biobest Group N V | Mite composition and method for growing mites |
| CN111700038A (en) * | 2020-06-24 | 2020-09-25 | 潮州中天农业科技有限公司 | Anti-infection method for predatory mites |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101049098A (en) * | 2007-03-15 | 2007-10-10 | 福建农林大学 | Culture medium for preserving species of mites |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101268772B (en) * | 2008-05-23 | 2012-03-21 | 北京依科曼生物技术有限公司 | Predatory mites producing method |
| CN106720973A (en) * | 2015-11-20 | 2017-05-31 | 刘振宇 | A kind of organic standard pig premix and preparation method thereof |
-
2017
- 2017-06-12 CN CN201710439340.8A patent/CN107114620B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101049098A (en) * | 2007-03-15 | 2007-10-10 | 福建农林大学 | Culture medium for preserving species of mites |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107114620A (en) | 2017-09-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR100749227B1 (en) | Method for preparing fermented microbial feed additives for livestock using mushroom media and fermented microbial feed for livestock using the same | |
| KR101010762B1 (en) | Biologically Controlled Strains and Their Microbial Organic Fertilizers for Withering Disease of Cucumber and Watermelon | |
| Thiribhuvanamala et al. | Improved techniques to enhance the yield of paddy straw mushroom (Volvariella volvacea) for commercial cultivation | |
| CN101875571B (en) | Method for preparing enhanced liquid microbial organic fertilizer | |
| CN105296394A (en) | Microbial decomposition agent for animal wastes and straws and preparation method thereof | |
| US10080333B2 (en) | Hydroponic method utilizing beneficial micro-organisms | |
| CN102775202A (en) | Novel microbial organic fertilizer with anti-actinomyces active compound bacteria and preparation method thereof | |
| CN107266183A (en) | Chicken manure organic fertilizer and preparation method thereof | |
| CN102617203A (en) | Method for producing microbial organic fertilizer by utilizing poultry feathers and product thereof | |
| CN103651582A (en) | Broad-spectrum composite microbial fungicide and preparation method thereof | |
| Carrasco et al. | Commercial cultivation techniques of mushrooms | |
| CN107188724B (en) | Fermentation process of biological organic bacterial fertilizer | |
| CN107114620B (en) | Predatory mite culture base material and using method thereof | |
| KR20050111367A (en) | A pgpr growing-promoter and it's growing method for crops | |
| KR100727529B1 (en) | The livestock feeds and the method of livestock feeds production using edible mushroom mycelia | |
| KR101892935B1 (en) | Compostion for Improving Bottom Materials and Growth Enhancing | |
| CN107410367A (en) | A kind of paecilomyces fumosoroseus finish and preparation method thereof and a kind of insecticide | |
| WO2020168649A2 (en) | Citrus greening disease prevention method | |
| KR20180007244A (en) | Commposition for accelerating growth of plants using bacillus amyloliquefaciens and use thereof | |
| JP5833893B2 (en) | Sterilization method of organic fertilizer | |
| CN101473855B (en) | Propagating agent for preventing fungus in field | |
| KR102430672B1 (en) | Fertilizer manufacturing method using indigenous microorganisms | |
| KR101687891B1 (en) | Cultivating method of tree ear and the composition of cultur medium | |
| WO2020168650A1 (en) | Microorganism composite formulation and preparation method therefor | |
| CN107227270A (en) | A kind of biocontrol bacteria in soil-borne diseases agent, a kind of soil-borne disease biological control matrix and preparation method thereof and application method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201002 |