CN107094805A - A kind of plant pesticide composition comprising camphor tree leaf essential oil and its application - Google Patents
A kind of plant pesticide composition comprising camphor tree leaf essential oil and its application Download PDFInfo
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- CN107094805A CN107094805A CN201710302026.5A CN201710302026A CN107094805A CN 107094805 A CN107094805 A CN 107094805A CN 201710302026 A CN201710302026 A CN 201710302026A CN 107094805 A CN107094805 A CN 107094805A
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- Prior art keywords
- essential oil
- camphor tree
- tree leaf
- leaf essential
- active component
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/24—Lauraceae [Laurel family], e.g. laurel, avocado, sassafras, cinnamon or camphor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention belongs to pesticides application technical field, and in particular to a kind of plant pesticide composition comprising camphor tree leaf essential oil and its application.A kind of plant pesticide composition for including camphor tree leaf essential oil, comprising active components A and active component B, active components A is camphor tree leaf essential oil, and active component B is Osthole, and active components A and active component B weight ratio are 1:30‑30:1.The composition pesticide of the present invention has obvious synergistic effect, reduces dosage, can effectively prevent and treat the insect that resistance has been produced to existing medicament;The dispenser of composition pesticide of the present invention simultaneous can control various pests, reduce drug cost.
Description
Technical field
The invention belongs to pesticides application technical field, and in particular to a kind of botanical pesticide combination comprising camphor tree leaf essential oil
Thing and its application.
Background technology
Camphor tree (Cinnamomum camphora) also known as cinnamomum camphora, black camphor tree, camphorwood, fragrant camphor tree etc., are that Lauraceae Cinnamomum is evergreen tall and big
Broad-leaved arbor.Camphor tree is one of China natural perfume material seeds, and the position such as its root, stem, leaf, fruit, which can be refined, isolates essence
Oil comes.The chemical composition of camphor tree essential oil is very abundant, the chemical composition for having about 300 kinds in camphor tree leaf essential oil.Camphor tree leaf essential oil
It is main into including the chemical combination such as linalool, safrole, camphor, borneol, nerolidol, different nerolidol, citral, geraniol
Thing;Most of chemical compositions play the role of sterilization, desinsection, antibacterial, in industries such as light industry, perfumery industry, beauty culture, pharmaceutical sectors
It is widely used, with wide exploitation prospect.
Osthole also known as osthole, are the one kind extracted from the fruit of Umbelliferae frutus cnidii platymiscium cnidium monnieri
Coumarin kind compound, its chemical name is osthole, is that content highest is (about in frutus cnidii
1%) a kind of alkyl Coumarins active chemical.Medicine lead its domain there is anti-hypertension, it is anti-arrhythmia, anti-ageing
Always, the effects such as antitumor, anti-osteoporosis disease, anti-inflammatory and antiallergic action;In agriculture field, it has desinsection, the work of sterilization
With.
Chemical pesticide is used for a long time, causes Heavy environmental pollution, drug-fast increase, exploitation has desinsection wide spectrum, low toxicity,
The botanical pesticide for the advantages of being not likely to produce the resistance to the action of a drug and good Environmental compatibility is current hot subject.In addition by different structure
The pesticide active ingredient of type is compounded, and is current one kind the problems such as solve cost and resistance in agricultural chemicals single dose application process
Effective means.Compound synergic is formulated well, can improve actual prevention effect, is reduced the usage amount of agricultural chemicals, is helped to delay to resist
The generation of property, is the important means of scientific prevention and cure pest and disease damage.At present at home and abroad also without camphor tree leaf essential oil and nimbin, cnidium monnieri
The relevant report of sub- element compounding.
The content of the invention
It is an object of the invention to provide a kind of plant pesticide composition with notable synergistic effect, with low toxicity, environment
Friendly the characteristics of.
It is another object of the present invention to provide a kind of new application for synergistic composition.
To overcome the deficiencies in the prior art, inventor is tested by a large amount of indoor bioassay, has been surprisingly found that camphor tree folic acid
After oil and nimbin or Osthole are compounded with certain proportion, diamondback moth, tea geometrid are had significant synergies.
In the present invention, plant pesticide composition includes active components A and active component B, and active components A is camphor tree folic acid
Oil, active component B is Osthole, and active components A and active component B weight ratio are:1:30-30:1.
Further, when active component B is Osthole, active components A and active component B weight ratio are 1:15-
30:1。
Present invention also offers a kind of pesticidal preparations for including described plant pesticide composition, it is characterised in that also
Include agriculturally acceptable auxiliary agent.
Preferably, in described pesticidal preparations, active components A and active component B weight percentage are 0.1-
40%.
Preferably, the formulation of described pesticidal preparations is missible oil, suspending agent, wettable powder, water dispersible granules, water and milk
Agent or microemulsion.
It is used for the purposes for preventing and treating crop pest present invention also offers described pesticidal preparations.
It is used for the purposes for preventing and treating diamondback moth, tea geometrid present invention also offers described pesticidal preparations.
The extracting method of camphor tree leaf essential oil of the present invention:
The blade (tender leaf, old leaf or fresh fallen leaves) of camphor tree is gathered first, and after cleaning, leaf surface moisture is dried naturally, it is stand-by;
Next weighs a certain amount of material handled well, shreds and is put into round-bottomed flask, steam distillation is used in volatile oil extractor
Method is extracted, distillation time 4h, essential oil anhydrous Na2SO4Dry, obtain the sealing of camphor tree leaf essential oil stand-by.
Compared with prior art, the beneficial effects of the invention are as follows:
(1) composition pesticide of the invention has obvious synergistic effect, reduces dosage, can effectively prevent and treat to existing
Medicament has produced the insect of resistance.
(2) dispenser of composition pesticide of the invention simultaneous can control various pests, reduce drug cost.
(3) composition pesticide of the invention is by mixture, and action site increase is conducive to overcoming and delays insect generation anti-
The property of medicine, extends the service life of medicament.
(4) active component of composition pesticide of the invention is botanical pesticide, with low toxicity, is not likely to produce the resistance to the action of a drug
With Environmental compatibility it is good the advantages of.
Embodiment
The present invention is described in detail its advantage with specific examples below.
First, indoor virulence test is determined
Biologicall test example 1:Camphor tree leaf essential oil and nimbin composition are to wild cabbage diamondback moth Toxicity Determination
Subjects:Wild cabbage diamondback moth
Method of testing is blade medicine embrane method.The fresh cabbage leaves without pollution by pesticides are taken to be immersed in the camphor tree folic acid of series concentration
The middle 10s of oil and nimbin, is compared, interior is dried with distilled water.The training that appropriately sized disk is placed in 9cm is cut into after drying
Support in ware, access 3 instar larvae of the same size, each 3~4 repetitions of concentration, each repeatedly 10~20 larvas are united after 48h
The death rate is counted, concentration LC50 values in suppressing are calculated with least square method.Again co-toxicity coefficient (CTC) is calculated according to the abundant methods of Sun Yun.Work as CTC
< 80, then composition show as antagonism, as 80 < CTC < 120, then composition shows as summation action, as CTC > 120,
Then composition shows as synergistic effect.
Survey toxicity index (ATI)=(standard agent LC50/ reagent agent LC50) × 100
A percentage composition+B medicament toxicity index in theoretical toxicity index (TTI)=A medicament toxicity index × mixture × mixed
B percentage composition in agent
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theoretical toxicity index (TTI)] × 100;Poison
Power measurement result is as shown in table 1.
The camphor tree leaf essential oil of table 1 and nimbin composition are to wild cabbage diamondback moth indoor virulence
As shown in Table 1, camphor tree leaf essential oil and nimbin are in specific ratio range, and composition has aobvious to wild cabbage diamondback moth
Write synergistic effect;When the quality proportioning of camphor tree leaf essential oil and nimbin is 1:10-15:In the range of 1, its co-toxicity coefficient is more than
120, show notable synergistic effect.
Biologicall test example 2:Camphor tree leaf essential oil and Osthole composition are to tea geometrid Toxicity Determination
Subjects:Tea geometrid
Method of testing is leaf dipping method.Transplant and dry after fresh tea blade tip is impregnated in decoction, cut the tea tip dried and put
Enter in can, every bottle of 3-4 tea tip, select in 2 instar larvae access can of the same size, each processing connects worm 15,
In triplicate, 26-27 DEG C of temperature control, and suitable humidity is kept, feed fresh normal blade.120h investigates its death after medicine
Rate, concentration LC50 in suppressing is calculated with least square method, then calculate co-toxicity coefficient (CTC) according to the abundant methods of Sun Yun.As CTC < 80, then
Composition shows as antagonism, as 80 < CTC < 120, then composition shows as summation action, as CTC > 120, then composition
Show as synergistic effect.
Survey toxicity index (ATI)=(standard agent LC50/ reagent agent LC50) × 100
A percentage composition+B medicament toxicity index in theoretical toxicity index (TTI)=A medicament toxicity index × mixture × mixed
B percentage composition in agent
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theoretical toxicity index (TTI)] × 100;Poison
Power measurement result is as shown in table 2.
The camphor tree leaf essential oil of table 2 and Osthole composition are to tea geometrid indoor virulence
As shown in Table 2, camphor tree leaf essential oil and nimbin are in specific ratio range, and composition has aobvious to tea tree tea geometrid
Write synergistic effect;When the quality proportioning of camphor tree leaf essential oil and nimbin is 1:15-30:In the range of 1, its co-toxicity coefficient is more than
120, show notable synergistic effect.
Pesticidal combination of the present invention can be prepared into the preparation for being adapted to agriculturally use with known method.It is as described below only
For preferably embodiment of the invention, only to explain the present invention, therefore it can not be interpreted as to the scope of the claims of the present invention
Limitation.
2nd, agricultural chemicals prepares embodiment
Embodiment 1:
Camphor tree leaf essential oil 10%, nimbin 1%, 1-METHYLPYRROLIDONE 4%, agriculture breast 1601#6%, Tween-60 #3%,
Agriculture breast 2201#4%, epoxychloropropane 7%, benzoic acid 2%, propane diols 2%, deionized water supplies 100%.Mentioned component is pressed
Conventional method processing can be prepared by 11% camphor tree leaf essential oil nimbin aqueous emulsion.
Embodiment 2:
Camphor tree leaf essential oil 5%, Osthole 1%, 1-METHYLPYRROLIDONE 5%, agriculture breast 1601#5%, Tween-60 #3%,
Agriculture breast 2201#3%, epoxychloropropane 6%, benzoic acid 4%, propane diols 2%, deionized water supplies 100%.Mentioned component is pressed
Conventional method processing can be prepared by 6% camphor tree leaf essential oil osthole water-emulsion agent.
Embodiment 3:
Camphor tree leaf essential oil 2%, nimbin 2%, 1-METHYLPYRROLIDONE 12%, agriculture breast 1601#10%, sodium alkyl sulfonate
15%th, organosilicon 2%, deionized water supplies 100%.Mentioned component is processed according to a conventional method can be prepared by 4% camphortree leaf
Essential oil micro nimbin emulsion.
Embodiment 4:
Camphor tree leaf essential oil 0.4%, Osthole 2%, 1-METHYLPYRROLIDONE 7%, agriculture breast 1601#11%, alkyl sulfonic acid
Sodium 20%, organosilicon 3%, deionized water supplies 100%.Mentioned component is processed according to a conventional method can be prepared by 2.4% camphor tree
Leaf essential oil Osthole microemulsion.
Embodiment 5:
Camphor tree leaf essential oil 4%, nimbin 2%, 1-METHYLPYRROLIDONE 12%, agriculture breast 500#6%, dimethylbenzene to 100%.
Mixed in the method input mixing kettle that above raw material is routinely prepared to missible oil, it is 6% that effective Ingredients Weight percentage composition, which is made,
Camphor tree leaf essential oil nimbin emulsion.
Embodiment 6:
Camphor tree leaf essential oil 7%, Osthole 2%, 1-METHYLPYRROLIDONE 14%, agriculture breast 500#9%, dimethylbenzene is extremely
100%.Mixed in the method input mixing kettle that above raw material is routinely prepared to missible oil, effective Ingredients Weight percentage composition is made
For 9% camphor tree leaf essential oil Osthole EC.
3rd, field efficacy is tested
Field efficacy example 1:Camphor tree leaf essential oil and nimbin composition are to wild cabbage diamondback moth field efficacy
Experimental subjects:Wild cabbage diamondback moth
Test medicine and dosage refer to table 3.Arranged using random district's groups, often processing is repeated 3 times, plot area 20m2, often
Advise knapsack hand sprayer even spraying.Spraying time is that wild cabbage rolls into a ball a phase, field diamondback moth occurance peak.During experiment with
Based on cloudy weather, 22 DEG C of daily mean temperature.Pilot survey is using the method for pinpointing singling.Per cell by 5 points of samplings of diagonal,
Investigate the quantity of each instar larvae of complete stool.1d, 3d, 7d after insect population, medicine are investigated before medicine and investigates work worm remaining on singling wild cabbage
Number, calculates its preventive effect, and seen whether that poisoning occurs.
The camphor tree leaf essential oil of table 3 and nimbin composition are to wild cabbage diamondback moth field efficacy
As shown in Table 3, camphor tree leaf essential oil and nimbin composition have notable synergistic effect to wild cabbage diamondback moth, and preventive effect reaches
To more than 92%, it is possible to reduce the amount of application of active component.Produced without poisoning.
Field efficacy example 2:The field efficacy of camphor tree leaf essential oil and Osthole to tea geometrid
Subjects:Tea geometrid
Test medicine and dosage refer to table 4.Arranged using random district's groups, often processing is repeated 4 times, plot area 30m2, altogether
Count 20 cells.With knapsack hand sprayer in dispenser.It is 60kg per 667m2 water consumptions, tea leaf is uniformly squirted.Apply
Medicine, which is worked as, to be become a fine day, 22~30 DEG C of temperature, relative humidity 46%, without rainfall in 3d after dispenser.
In before dispenser and dispenser after 1,3,7d respectively investigation once, full period is investigated 4 times altogether.Per cell 2 sampling points of mark, every point
0.2m2, calculates Revision insect recluced rate and preventive effect.
Calculation formula is:
The field efficacy of the camphor tree leaf essential oil of table 4 and Osthole to tea geometrid
As shown in Table 4, camphor tree leaf essential oil and nimbin composition have notable synergistic effect to tea geometrid, and preventive effect reaches
More than 91%, it is possible to reduce the amount of application of active component.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not limited to the substantial technological content of the present invention
Scope, substantial technological content of the invention is broadly to be defined in the right of application, any skill that other people complete
Art or method, if it is identical with defined in claims hereof scope, or a kind of simply equivalent replacement,
It is considered as being covered by among the right.
Claims (7)
1. a kind of plant pesticide composition for including camphor tree leaf essential oil, it is characterised in that include active components A and active component
B, active components A is camphor tree leaf essential oil, and active component B is Osthole, and active components A and active component B weight ratio are 1:
30-30:1。
2. plant pesticide composition according to claim 1, it is characterised in that active component B is Osthole, and
Active components A and active component B weight ratio are 1:15-30:1.
3. a kind of pesticidal preparations of the plant pesticide composition comprising described in claim any one of 1-2, it is characterised in that also
Include agriculturally acceptable auxiliary agent.
4. pesticidal preparations according to claim 3, it is characterised in that active components A and active component B weight percent contain
Measure as 0.1-40%.
5. pesticidal preparations according to claim 3, it is characterised in that the formulation of described pesticidal preparations is missible oil, suspended
Agent, wettable powder, water dispersible granules, aqueous emulsion or microemulsion.
6. the pesticidal preparations according to claim any one of 3-5 are used for the purposes for preventing and treating crop pest.
7. pesticidal preparations according to claim 6 are used for the purposes for preventing and treating diamondback moth, tea geometrid.
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Cited By (1)
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Application publication date: 20170829 |