CN107668078B

CN107668078B - Synergistic pesticide composition containing chenopodium ambrosioides essential oil and application thereof

Info

Publication number: CN107668078B
Application number: CN201711061770.7A
Authority: CN
Inventors: 张方梅; 乔利; 潘鹏亮; 陈俊华; 智亚楠; 王国君; 周顺玉
Original assignee: Xinyang Agriculture and Forestry University
Current assignee: Xinyang Agriculture and Forestry University
Priority date: 2017-11-02
Filing date: 2017-11-02
Publication date: 2020-09-25
Anticipated expiration: 2037-11-02
Also published as: CN107668078A

Abstract

The application belongs to the field of pesticides, and particularly relates to a synergistic pesticide composition containing chenopodium ambrosioides essential oil and application thereof. A synergistic pesticide composition containing chenopodium ambrosioides essential oil and bifenthrin, wherein the mass ratio of the chenopodium ambrosioides essential oil to the bifenthrin is 1:15-25:1, and the chenopodium ambrosioides essential oil and bifenthrin can be used for controlling tea geometrid. The pesticide composition can effectively prevent and control tea geometrid and is safe to tea trees; and can reduce the dosage and slow down the generation of drug resistance.

Description

Synergistic pesticide composition containing chenopodium ambrosioides essential oil and application thereof

Technical Field

The application belongs to the field of pesticides, and particularly relates to a synergistic pesticide composition containing chenopodium ambrosioides essential oil and application thereof.

Background

Tea geometrid (Ectropis obliqua Prout) belongs to Lepidoptera and family of Bombycidae, and is one of the most common and serious pests in tea gardens. Currently, the control of this insect relies primarily on chemical and biological control. Because a great amount of chemical pesticide is used for preventing and controlling the tea geometrid, the resistance of the tea geometrid to the chemical pesticide is gradually increased, the environment is polluted, the natural enemies of a tea garden are greatly reduced, and the like, so that the ecological balance is damaged. Meanwhile, the long-term residue accumulation of the pesticide causes the food safety problem of the tea leaves and the human health problem.

At present, when different chemical components are matched with different plant essential oils for use, the effective components contained in the plant essential oils may show synergistic action, and the use concentration of chemical pesticides can be reduced to a certain extent. Meanwhile, as a plurality of components act on pests synergistically, the action mechanism is complex, and the pests are not easy to generate drug resistance. Thus, plant essential oils not only become one of the important sources of pesticides, but also have the potential to act as synergists for chemical pesticides. Some researches show that some plant secondary biomass has obvious synergistic effect on pesticides. The ethanol extracts of the plants such as pepper, artemisia pigweed, pseudoforsythia and the like and the tea saponin have obvious synergistic effect on the insecticidal activity of Bt (Mohan & Gujar.2003; Sayyed & Wright, 2004; Li et al, 2006); sophora alopecuroides extract, fennel oil chemical components, myristicin and the like can improve the efficacy of organic phosphorus (Lieben et al, 2006; Zhao Jian Wei et al, 2007); the plant essential oils of pepper, mugwort, masson pine needle, juniper, yellow wormwood, etc. can improve the penetration of fipronil, abamectin and chlorpyrifos to the epidermis of diamondback moth larvae (sun-Hongxia, 2008).

In order to solve the problems in the prior art, the invention selects the essential volatile oil of tea plant pest tea geometrid and chenopodiaceae plant chenopodium ambrosioides as a research object, develops a synergistic composition to reduce the usage amount of chemical pesticides in a tea garden, provides a certain theoretical basis for the development of green prevention and control technology of the tea garden, and ensures the safe production of tea products.

Disclosure of Invention

In order to solve the problems that the tea geometrid has gradually increased resistance to chemical pesticides, pollutes the environment, greatly reduces natural enemies of a tea garden and the like in the prior art, the invention provides the synergistic pesticide composition containing the chenopodium ambrosioides essential oil, and the synergistic pesticide composition can be used for preventing and controlling the tea geometrid of the tea trees.

The technical scheme provided by the invention is as follows;

a synergistic pesticide composition containing chenopodium ambrosioides essential oil comprises chenopodium ambrosioides essential oil and bifenthrin, wherein the mass ratio of the chenopodium ambrosioides essential oil to the bifenthrin is 1:15-25: 1.

The pesticide composition comprises the effective components of chenopodium ambrosioides essential oil and bifenthrin in a mass ratio of 1:10-20:1, preferably in a mass ratio of 1:5-10: 1.

The pesticide composition can be prepared into a preparation acceptable for pesticides, and the total weight of the chenopodium ambrosioides essential oil and the bifenthrin in the preparation accounts for 5-90 percent of the total mass of the preparation, preferably 25-60 percent.

The pesticide composition of the invention is in the form of missible oil, aqueous emulsion and the like.

The pesticide composition provided by the invention is used for preventing and controlling tea geometrid.

Compared with the prior art, the invention has the following beneficial effects:

(1) as known from a large number of indoor and field pesticide effect tests, the effect of the pesticide composition is not simple superposition of the activities of all components, but shows obvious synergistic effect.

(2) The pesticide composition can be prepared into preparations of different formulations such as missible oil, aqueous emulsion and the like, each preparation has good safety to crops, meets the safety requirement of pesticide preparations, and is suitable for popularization and application.

(3) The pesticide composition can effectively prevent and control tea geometrid and is safe to tea trees; and can reduce the dosage and slow down the generation of drug resistance.

Detailed Description

The following detailed description of specific embodiments of the present invention is provided in conjunction with examples, but it should be understood that the scope of the present invention is not limited to the specific embodiments.

Experiment one: determination of contact toxicity activity of chenopodium ambrosioides essential oil and bifenthrin compounded and paired tea geometrid

Test insects: 3-instar larvae of tea geometrid collected from field

Test reagents: chenopodium ambrosioides essential oil: cleaning herba Chenopodii, cutting into pieces, air drying, cutting into small segments, distilling in steam distiller to extract volatile oil, and storing the obtained herba Chenopodii essential oil. Bifenthrin raw material: purchased from the market.

The test method comprises the following steps: micro-dropping method is adopted. Dripping 5 μ l medicinal liquid on the forebreast back plate of the test insect with 5 μ l microsyringe, treating 20 head insect, repeating for 4 times, feeding fresh folium Camelliae sinensis in artificial climate box, treating with distilled water, checking the result 7d after treatment, and calculating LC50 value.

The co-toxicity coefficient (CTC) was calculated according to the Sun cloud Peter method. When CTC is less than 80, the composition is antagonistic, when CTC is less than 80, the composition is additive, and when CTC is less than 120, the composition is synergistic.

Measured virulence index (ATI) ═ standard agent EC 50/test agent EC50 x 100

Theoretical virulence index (TTI) ═ A agent virulence index x percent of A in the mixture + B agent virulence index x percent of B in the mixture

Co-toxicity coefficient (CTC) ═ measured toxicity index (ATI) of the mixture/Theoretical Toxicity Index (TTI) of the mixture x 100

TABLE 1 determination of the contact toxicity of Chenopodium ambrosioides essential oil and bifenthrin in combination with ectropis obliqua

As can be seen from Table 1, the compound of chenopodium ambrosioides essential oil and bifenthrin has a significant contact killing synergistic activity within the mass ratio of 1:15-25:1, the preferred mass ratio is 5:1, and the synergistic CTC value is 188.0.

Experiment two: field control effect of compound chenopodium ambrosioides essential oil and bifenthrin on ectropis obliqua

Example 1:

weighing 10g of chenopodium ambrosioides essential oil, 2g of bifenthrin, 7.5g of calcium dodecyl benzene sulfonate, 4g of sodium dodecyl sulfate, 15g of phenethyl phenol polyoxyethylene ether and 100g of 150# solvent oil. The raw materials are put into a mixing kettle to be mixed according to a conventional method for preparing missible oil, and the missible oil with the weight percentage content of the effective components of 12 percent is prepared.

Example 2:

weighing 25g of chenopodium ambrosioides essential oil, 25g of bifenthrin, 3g of phenethyl phenol polyoxyethylene ether phosphate, 5g of polyoxyethylene polyoxypropylene ether sulfate, 33#2g of agricultural emulsion and 13g of 150# solvent oil, and adding water to 100 g. The raw materials are put into a mixing kettle according to a conventional method for preparing the aqueous emulsion and are mixed at high speed to prepare the aqueous emulsion with the weight percentage of the effective components of 50 percent.

The test method comprises the following steps: uniformly spraying the tea trees by using a manual sprayer, taking the wetting degree of the surfaces of the leaves as the degree, examining the population base numbers of the tea geometrid at the age of 2-3 days before and after the application, and calculating the control effect. The results are shown in Table 2.

TABLE 2 field control of Chenopodium ambrosioides essential oil and bifenthrin in combination with ectropis obliqua

As can be seen from Table 2, compared with the single use, the compound of the chenopodium ambrosioides essential oil and the bifenthrin can improve the control effect of the chenopodium ambrosioides essential oil on tea geometrid, has obvious synergistic effect, reduces the use dosage of the medicament and slows down the generation of drug resistance.

The present invention has been further described with reference to the examples, but the present invention is not limited to the above-described embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.

Claims

1. Use of a synergistic pesticide composition comprising chenopodium ambrosioides essential oil for controlling tea geometrid, characterized in that: the composition contains effective components chenopodium ambrosioides essential oil and bifenthrin, wherein the mass ratio of the effective components chenopodium ambrosioides essential oil to the bifenthrin is 1:15-25: 1; the mass of the chenopodium ambrosioides essential oil and the bifenthrin in the preparation accounts for 5-90% of the total mass of the whole pesticide preparation, and the balance is an agriculturally and pharmaceutically acceptable auxiliary material.

2. Use according to claim 1, characterized in that: the mass ratio of the effective component chenopodium ambrosioides essential oil to bifenthrin is 1:10-20: 1.

3. Use according to claim 1, characterized in that: the mass ratio of the effective component chenopodium ambrosioides essential oil to bifenthrin is 1:5-10: 1.

4. Use according to claim 1, characterized in that: the pesticide preparation is in the form of missible oil or aqueous emulsion.