CN107088213A - Chinese medical extract, cream for preventing and alleviating senile skin chap and its production and use - Google Patents
Chinese medical extract, cream for preventing and alleviating senile skin chap and its production and use Download PDFInfo
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/46—Eucommiaceae (Eucommia family), e.g. hardy rubber tree
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
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- A61K2236/50—Methods involving additional extraction steps
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Abstract
The invention discloses a kind of Chinese medical extract, cream for being used to preventing and alleviating senile skin chap and its production and use, belong to daily cosmetics studying technological domain.The present invention is directed to the elderly's skin characteristic, it is proposed that a kind of Chinese medical extract and the cream prepared by the extract, the Chinese medical extract are to extract to obtain from the Chinese medicine composition being made up of the bulk drug of following parts by weight：10~20 parts of 20~40 parts of teasel root, 15 30 parts of the bark of eucommia, 15~30 parts of gumbo, 10~20 parts of sealwort and the bark of official magnolia.Raw material of the present invention select natural plants, non-stimulated to skin safe.Experiment proves that the extract and cream of the present invention not only has mitigation to chap, also with anti-inflammation, moisturizing profit flesh, Shu Min is antipruritic the effects such as, and skin barrier can be repaired, fundamentally treat the skin problems such as old chap, itch.Easy, preparation method simplicity that raw material of the present invention are obtained, green safety, alleviates and prevention chap effect is good, with good application prospect and market prospects.
Description
Technical field
The skin care formulation prepared the present invention relates to a kind of Chinese medical extract and by the extract, it is more particularly to a kind of
For the cream prevented and alleviated the Chinese medical extract of senile skin chap and prepared by the Chinese medical extract and its preparation
Method.The invention belongs to daily cosmetics studying technological domain.
Background technology
Fissura, also known as rhagadia manus et pedis, refer to that the dry skin and wire of hand, pedal skin caused by a variety of causes are split
A kind of disease of gap.Modern medicine believes that, when epidermal keratinocyte dehydration is more than 10%, it is possible to occur chapped skin.Chap
It is a kind of common skin symptom.The elderly is because of organ aging, and subcutaneous sweat gland secretion is reduced, and dry skin, skin keratin is thickened, and loses
Elasticity and extensibility are gone, the stimulation of mechanicalness or chemical substance is more susceptible to, winter climate is cold in addition, therefore when extremity movement
It is more easy to chap than young man.
Skin care item on the market are generally young man's design at present, have ignored the skin demand of the elderly.The elderly can only select
Select the skin care item that young man is applicable.And the skin of the elderly and the skin properties of young man have dramatically different, nursing care mode
There is difference.The cosmetics that the elderly is applicable using young man, it is impossible to reach obvious skin effect.
Therefore, be badly in need of at present it is a kind of for the elderly design, safely and effectively can prevent and alleviate the elderly's skin
The skin care formulation of chap.
The content of the invention
An object of the present invention is to provide a kind of Chinese medicine composition for being used to preventing and alleviating senile skin chap.
The second object of the present invention be to provide a kind of Chinese medical extract prepared by above-mentioned Chinese medicine composition and its
Preparation method.
The third object of the present invention is to propose a kind of skin care formulation prepared based on above-mentioned Chinese medical extract.
To realize the purpose of the present invention, present invention employs following technical scheme：
The present invention utilizes advanced biotechnology, with reference to the theory of traditional Chinese medical science of Chinese tradition, by teasel root, the bark of eucommia, gumbo, Huang
Essence, the bark of official magnolia are effectively combined in together, and decide its consumption, so as to form compound preparation.In addition, the present invention makees its extract solution
For active component, add in the cream of preventing and treating chap, research shows, Chinese medical extract of the invention or prepared by it
Cream has protection with repairing skin barrier, anti-inflammatory, antipruritic effect, and safe and non-stimulating, gentle so as to effectively prevention
The old chap of solution, improves senile skin vigor, recovers senile skin health.
Wherein, teasel root, Chinese medicine name.For Dipsacaceae herbaceos perennial teasel (Dipsacus asperoides
C.Y.Cheng et T.M.Ai) root, there is hemostatic analgesia.
The bark of eucommia (Eucommia ulmoides), is the dry bark of the Eucommiaceae plant bark of eucommia, is Chinese rare tonic
Material.Its is sweet, warm-natured.Contain gutta-percha, glucoside, alkaloid, pectin, fat, resin, organic acid and the life of a variety of dimensions in bark
Element and the necessary trace element of human body.
Sealwort (Polygonatum sibiricum) is the plant of Liliaceae Polygonatum.Rhizome can be used as medicine.Sealwort rhizome contains
Nicotinic acid, mucilaginous substance, quinones.And the first, second containing Siberian solomonseal rhizome polysaccharide, third (being combined into by glucose, mannose, galacturonic acid),
Polygonatum rhizome oligosaccharide first, second, third (being combined into by glucose and fructose).In addition, separately containing 11 kinds of amino acid such as lysines.
Gumbo (Abelmoschus esculentus) is also known as okra, Abelmoschus esculentus, popular name swordweed, purling eggplant, gumbo
Fruit is containing pectin, cow's milk glycan etc., with helping digest, the effect of treat gastritis and gastric ulcer, protect skin and stomach lining.
The bark of official magnolia is the Magnoliacea plant bark of official magnolia (Magnolia officinalis Rehd.et Wils) or Magnolia bilola
The drying dry hide of (Magnolia officinalis Rehd.et Wils.var.biloba Rehd.et Wils.), root skin and
Branch skin.Magnolia bark contains Neolignans, and Neolignans, alkaloid, volatilization drop in monoterpene Neolignans
Oil etc., has effects that promoting the circulation of qi dampness elimination, warm in pain.
Specifically, a kind of Chinese medicine composition for being used to preventing and alleviating senile skin chap of the present invention, it is by following heavy
Measure each bulk drug composition of part：The parts by weight of teasel root 20~40, bark of eucommia 15-30 parts by weight, the parts by weight of gumbo 15~30, sealwort 10~
20 parts by weight and the parts by weight of the bark of official magnolia 10~20.
In the present invention, it is preferred to, described Chinese medicine composition is made up of each bulk drug of following parts by weight：The weight of teasel root 40
Measure part, the parts by weight of the bark of eucommia 30, the parts by weight of gumbo 20, the parts by weight of sealwort 15 and the parts by weight of the bark of official magnolia 15.
A kind of Chinese medical extract for being used to preventing and alleviating senile skin chap, it is by Chinese medicine composition of the present invention
Extract and obtain by the following method：
(1) after each bulk drug is crushed, parts by weight as described above weigh each bulk drug, mix, obtain each bulk drug
Mixture；
(2) the mixture concentration of volume percent for each bulk drug that step (1) is obtained is 50%~90% (v/v) second
The mass volume ratio of alcohol extracting, bulk drug mixture and ethanol is 1：10~1：50(g：ML), 55 DEG C~85 DEG C 1~4h of extraction,
Obtain extract solution；
(3) extract solution for obtaining step (2) is cooled to 20 DEG C~30 DEG C, and 100~200 mesh filter-cloth filterings obtain filtrate；
(4) added in the filtrate obtained to step (3) mixed with ethanol volume identical butanediol in step (2) or propane diols
It is molten uniform, the ethanol in solution is rotated out, is filtered by vacuum, filtrate is collected, produces.
In the present invention, it is preferred to, the concentration of volume percent of ethanol is 75% (v/v), bulk drug mixing in step (2)
The mass volume ratio of thing and ethanol is 1：20(g：mL).
In the present invention, it is preferred to, the condition of vacuum filtration is that 1.2 μm of filter plates are spread in Buchner funnel in step (4), is entered
Row vacuum filtration.
Further, the purposes the invention also provides described Chinese medical extract in external-use skin care preparation is prepared,
Wherein, it is preferred that described external-use skin care preparation has Shu Min, antipruritic, prevention and alleviates chapped skin and repair skin screen
Effect of barrier.
A kind of external-use skin care preparation for being used to preventing and alleviating senile skin chap of the present invention, it is by of the present invention
Chinese medical extract and skin care field customary adjuvant are made.
A kind of cream for being used to preventing and alleviating senile skin chap, it is by A phase, B phase, C phase, D phases and E phase compositions；
Wherein, A phases are by stereth -2, stereth -21, cetostearyl alcohol, Butyrospermum parkii fruit fat extract, ore deposit
Fat, caprylic/capric triglyceride, stearine, stearic acid, lanolin, dimethyl silicone polymer, ethylhexyl palmitate
And tocopherol acetate composition；
Wherein, B phases are made up of xanthans, glycerine, EDETATE SODIUM, niacinamide, allantoin, glycine betaine and water；
Wherein, C phases are made up of the Chinese medical extract described in α-mannosan aqueous solution and claim any one of 3-5；
Wherein, D phases are made up of emulsifying agent；
Wherein, E phases are made up of preservative；
Wherein, the weight/mass percentage composition of each raw material is as follows：
It is preferred that, the weight/mass percentage composition of each raw material is as follows：
In the present invention, it is preferred to, described cream is prepared in accordance with the following methods：
1) B phases：Xanthans is disperseed in advance with glycerine, is added to the water, 70 DEG C -95 DEG C, 10-60 points of insulation are heated to
Clock；
2) A phases：After A phases raw material is well mixed, 70 DEG C -95 DEG C are heated to, 10-60 minutes are incubated；
3) B phases raw material carries out that A phase raw materials are slowly added into B phases in homogeneous, homogenizing process using 2000-7000r/min
In, 2000-7000r/min homogeneous 1-10min add D phase raw materials, 1000-5000r/min homogeneous 1-5 minutes, stirring cooling；
4) C, E phase raw material are added when temperature is down to 30-45 DEG C, stirring is to being completely dissolved；
5) temperature drops to 25-30 DEG C, discharges, ageing；
6) it is filling, packaging, finished product.
According to different using approach, other external-use skin care preparations, such as Gel are can be made into after Chinese medicine composition of the present invention is extracted
Curry, emulsion etc..Above-mentioned formulation is not limited to, can be according to known method in skin care industry field, by above-mentioned Chinese medical extract
A variety of external preparations are made by known method.
Finally, the invention also provides the described cream for being used to preventing and alleviate senile skin chap Shu Min, it is antipruritic,
Purposes in prevention and alleviation chapped skin and reparation skin barrier, it is preferred that the applicable crowd of described cream is 50 weeks
Crowd more than year.Cream of the present invention recommends method：After daily morning and evening warm water cleaning, affected part is coated on, is chapped
Serious symptom person can increase on demand.
The beneficial outcomes of the present invention：
Raw material of the present invention select natural plant composition, non-stimulated to skin safe.Ground for the elderly's skin characteristic
The Chinese medical extract of hair not only has mitigation to chap in itself, also with anti-inflammation, moisturizing profit flesh, and Shu Min is antipruritic to wait work(
Effect, and skin barrier can be repaired, fundamentally treat the skin problems such as old chap, itch.Meanwhile, the present invention is to old age
Application on human skin has protective effect, the generation that the elderly can be prevented to chap.Easy, preparation method letter that raw material of the present invention are obtained
Just, green safety, alleviates and prevention chap effect is good, with good application prospect and market prospects.
Brief description of the drawings
Fig. 1 is the erythrocyte hemolysis result of the test for the Chinese medical extract that embodiment 1 is prepared；
Fig. 2 is the lost situation of change of moisture of skin after the anti-chap cream sample prepared using embodiment 5.
Embodiment
The invention will now be further described with reference to specific embodiments, advantages of the present invention and feature will be with description and
It is apparent.But embodiment is only exemplary, does not constitute any limitation to the scope of the present invention.Those skilled in the art should
It should be appreciated that, the details and form of technical solution of the present invention can be repaiied without departing from the spirit and scope of the invention
Change or replace, but these modifications and replacement are each fallen within protection scope of the present invention.
Medicinal material used in the present embodiment can be commercially available from China Medicament Group Corp., meet People's Republic of China's medicine
2010 editions standards of allusion quotation.Other preparation raw materials source is shown in Table 1：
Table 1
The preparation of the Chinese medical extract of the present invention of embodiment 1
(1) by teasel root, after the bark of official magnolia is crushed respectively, each bulk drug is weighed according to following weight respectively for the bark of eucommia, gumbo, sealwort,
It is well mixed：
Teasel root 20g, bark of eucommia 25g, gumbo 15g, sealwort 10g, bark of official magnolia 10g；
(2) extracted with concentration of volume percent for 50% (v/v) ethanol, the mass volume ratio between bulk drug and ethanol is
1：40(g：ML), 85 DEG C of extraction 4h；
(3) extract solution for obtaining step (2) is cooled to 30 DEG C, and 100 mesh filter-cloth filterings obtain filtrate；
(4) added in the filtrate obtained to step (3) it is miscible uniform with ethanol volume identical butanediol in step (2),
The ethanol in solution is rotated out, is filtered by vacuum, filtrate is collected, produces Chinese medical extract A.
Vacuum filtration condition is that 1.2 μm of filter plates are spread in Buchner funnel in the step (4), is filtered by vacuum.
The preparation of the Chinese medical extract of the present invention of embodiment 2
(1) by teasel root, after the bark of official magnolia is crushed respectively, each bulk drug is weighed according to following weight respectively for the bark of eucommia, gumbo, sealwort,
It is well mixed：
Teasel root 40g, bark of eucommia 30g, gumbo 20g, sealwort 15g, bark of official magnolia 15g；
(2) extracted with concentration of volume percent for 75% (v/v) ethanol, the mass volume ratio between bulk drug and ethanol is
1：20(g：ML), 70 DEG C of extraction 2h；
(3) extract solution for obtaining step (2) is cooled to 20 DEG C, and 200 mesh filter-cloth filterings obtain filtrate；
(4) added in the filtrate obtained to step (3) it is miscible uniform with ethanol volume identical butanediol in step (2),
The ethanol in solution is rotated out, is filtered by vacuum, filtrate is collected, produces Chinese medical extract B.
Vacuum filtration condition is that 1.2 μm of filter plates are spread in Buchner funnel in the step (4), is filtered by vacuum.
The preparation of the Chinese medical extract of the present invention of embodiment 3
(1) by teasel root, after the bark of official magnolia is crushed respectively, each bulk drug is weighed according to following weight respectively for the bark of eucommia, gumbo, sealwort,
It is well mixed：
Teasel root 30g, bark of eucommia 15g, gumbo 30g, sealwort 20g, bark of official magnolia 20g；
(2) extracted with concentration of volume percent for 90% (v/v) ethanol, the mass volume ratio between bulk drug and ethanol is
1：50(g：ML), 55 DEG C of extraction 1h；
(3) extract solution for obtaining step (2) is cooled to 25 DEG C, and 150 mesh filter-cloth filterings obtain filtrate；
(4) added in the filtrate obtained to step (3) it is miscible uniform with ethanol volume identical propane diols in step (2),
The ethanol in solution is rotated out, is filtered by vacuum, filtrate is collected, produces Chinese medical extract C.
Vacuum filtration condition is that 1.2 μm of filter plates are spread in Buchner funnel in the step (5), is filtered by vacuum.
The preparation of the cream of the present invention of embodiment 4
1st, raw material and proportioning (mass percent)
It is shown in Table 2：
Table 2
2nd, the preparation method of cream：
1) B phases：Xanthans is disperseed in advance with glycerine, is added to the water, 70 DEG C are heated to, 60 minutes are incubated；
2) A phases：It is well mixed after A phase raw materials are weighed up, is heated to 70 DEG C, is incubated 60 minutes；
3) B phases raw material carries out homogeneous using 2000r/min, A phase raw materials is slowly added into B phases, 2000r/min homogeneous
10min or so, adds D phase raw materials, 1000r/min homogeneous 1 minute or so, stirring cooling；
4) C, E phase raw material are added when temperature is down to 30 DEG C or so, stirring is to being completely dissolved；
5) temperature drops to 25 DEG C, discharges, ageing；
6) it is filling, packaging, finished product.
The preparation of the cream of the present invention of embodiment 5
1st, raw material and proportioning (mass percent)
It is shown in Table 3：
Table 3
2nd, the preparation method of cream：
1) B phases：Xanthans is disperseed in advance with glycerine, is added to the water, 85 DEG C are heated to, 30 minutes are incubated；
2) A phases：It is well mixed after A phase raw materials are weighed up, is heated to 95 DEG C, is incubated 10 minutes；
3) B phases raw material carries out that A phase raw materials are slowly added into B phases in homogeneous, homogenizing process using 7000r/min,
7000r/min homogeneous 1min or so, adds D phase raw materials, 5000r/min homogeneous 3 minutes or so, stirring cooling；
4) C, E phase raw material are added when temperature is down to 30 DEG C or so, stirring is to being completely dissolved；
5) temperature drops to 25 DEG C, discharges, ageing；
6) it is filling, packaging, finished product.
The preparation of the cream of the present invention of embodiment 6
1st, raw material and proportioning (mass percent)
It is shown in Table 4：
Table 4
2nd, the preparation method of cream：
1) B phases：Xanthans is disperseed in advance with glycerine, is added to the water, 95 DEG C are heated to, 10 minutes are incubated；
2) A phases：It is well mixed after A phase raw materials are weighed up, is heated to 80 DEG C, is incubated 20 minutes；
3) B phases raw material carries out that A phase raw materials are slowly added into B phases in homogeneous, homogenizing process using 4000r/min,
4000r/min homogeneous 5min or so, adds D phase raw materials, 3000r/min homogeneous 5 minutes or so, stirring cooling；
4) C, E phase raw material are added when temperature is down to 45 DEG C or so, stirring is to being completely dissolved；
5) temperature drops to 30 DEG C, discharges, ageing；
6) it is filling, packaging, finished product.
The Efficacy experiments of the product of the present invention of embodiment 7
First, the safety experiment of Chinese medical extract A, Chinese medical extract B, Chinese medical extract C and its cream
In order to assess whether the Chinese medical extract of the present invention has adverse reaction to human body, the present invention has carried out system
Safety evaluation is tested：Erythrocyte hemolysis experiment, the multiple Skin Irritation Test of mouse and human closed formula patch test.
(1) erythrocyte hemolysis is tested
Erythrocyte hemolysis test method is with reference to Red Blood Cell Test System, in European alternative certification
The heart (European Centre for the Validation of Alternative Methods, ECVAM), European Union authority
Method validation center provide.Research shows that erythrocyte hemolysis rate to a certain extent can be with the excitant of response sample, haemolysis
Rate is higher, shows that the potential excitant of sample is bigger.Embodiment 1 prepares the erythrocyte hemolysis of various concentrations Chinese medical extract sample
Experimental result is as shown in Figure 1.
Fig. 1 results are shown, in the concentration range of tested concentration 1%~10% (v/v), and embodiment 1 prepares traditional Chinese medicine extraction
Thing shows the red blood rate of extremely low red blood cell, shows to exist that potential excitant possibility is extremely low, and security is good.Invention
People prepares Chinese medical extract with embodiment 2, embodiment 3 and repeats erythrocyte hemolysis experiment, obtains identical effect.
(2) the multiple Skin Irritation Test of mouse
By the multiple Skin Irritation Test of mouse, it is determined that and evaluating whether Chinese medical extract locally has to mammal skin
Stimulation or corrosiveness and its degree.
Test method skin irritation/corrosion test method with reference to as defined in 2007 editions cosmetics health specifications.Will be by
Once (or multiple) is coated on the skin of animal subject examination thing, and in predetermined time interval, observation animal skin is locally pierced
Swash the degree of effect and scored.Using own control, to evaluate stimulation of the tested material to skin.Acute skin irritation
The property experimental observation time limit should be enough to evaluate the invertibity or irreversibility of the effect.
Experimental result shows that 14 days given the test agent of continuous smearing (add embodiment 1 and prepare (the v/ of Chinese medicine extract 5% respectively
V) and 10% (v/v)), mouse skin does not occur erythema and oedema phenomenon.Inventor prepares Chinese medicine with embodiment 2, embodiment 3
Extract repeats erythrocyte hemolysis experiment, obtains identical effect.
The present invention prepares Chinese medical extract security well, nonirritant.
(3) human closed formula patch test
Human body patch test method reference《Cosmetics health specification (2007 editions)》, embodiment 4, embodiment 5, embodiment 6
Cream counter sample 1, sample 2, sample 3 are prepared, closed patch is carried out respectively, sample is investigated and adverse reaction is caused to human body
Possibility.Bare substrate 1-3 is same with discrete phase used in sample 1-3, but blank base is made in the corresponding Chinese medical extract of water consumption substitution
Matter.
Skin adverse reaction classification is as shown in table 5.
The skin adverse reaction of table 5 is classified
According to table 5, dermoreaction feelings of 30 subjects in 30 minutes, 24h and 48h are observed using spot examination device is removed
Condition.Result of the test is as shown in table 6-8.
Table 6 removes 30 minutes observation results of spot examination device
| Sample number into spectrum | 0 grade | 1 grade | 2 grades | 3 grades | 4 grades |
| Blank | 30 | 0 | 0 | 0 | 0 |
| Sample 1 | 30 | 0 | 0 | 0 | 0 |
| Sample 2 | 28 | 2 | 0 | 0 | 0 |
| Sample 3 | 29 | 1 | 0 | 0 | 0 |
| Bare substrate 1 | 29 | 1 | 0 | 0 | 0 |
| Bare substrate 2 | 29 | 1 | 0 | 0 | 0 |
| Bare substrate 3 | 29 | 1 | 0 | 0 | 0 |
Table 7 removes spot examination device 24h observation results
| Sample number into spectrum | 0 grade | 1 grade | 2 grades | 3 grades | 4 grades |
| Blank | 30 | 0 | 0 | 0 | 0 |
| Sample 1 | 30 | 0 | 0 | 0 | 0 |
| Sample 2 | 30 | 0 | 0 | 0 | 0 |
| Sample 3 | 30 | 0 | 0 | 0 | 0 |
| Bare substrate 1 | 30 | 0 | 0 | 0 | 0 |
| Bare substrate 2 | 30 | 0 | 0 | 0 | 0 |
| Bare substrate 3 | 30 | 0 | 0 | 0 | 0 |
Table 8 removes spot examination device 48h observation results
| Sample number into spectrum | 0 grade | 1 grade | 2 grades | 3 grades | 4 grades |
| Blank | 30 | 0 | 0 | 0 | 0 |
| Sample 1 | 30 | 0 | 0 | 0 | 0 |
| Sample 2 | 30 | 0 | 0 | 0 | 0 |
| Sample 3 | 30 | 0 | 0 | 0 | 0 |
| Bare substrate 1 | 30 | 0 | 0 | 0 | 0 |
| Bare substrate 2 | 30 | 0 | 0 | 0 | 0 |
| Bare substrate 3 | 30 | 0 | 0 | 0 | 0 |
According to 2007《Cosmetics health specification》Requirement, judge prepared by the corresponding embodiment 4-6 of sample 1, sample 2, sample 3
Gained cream has no adverse reaction to human body skin.
Result of the test shows that cream of the invention has no adverse reaction to human body skin, and security is good.
2nd, Ku812 the cells IL-8, IL-6 that Chinese medical extract A, Chinese medical extract B, Chinese medical extract C are induced PAMCI
Influence
(1) experimental principle：
IL-6 is that the mononuclear macrophage activated in a kind of multifunctional cytokine, blood is its main source.It is also
Start systemic inflammatory reaction most strong cell factor.IL-8 is the cell factor secreted by Th1 cells, main mediated cell poison
Generated with the immune response auxiliary antibody of local inflammation-related, these effects can cause body local inflammation reaction.To inflammation because
Sub- IL-6 and IL-8 suppression show that Chinese medical extract has and suppress scytitis, and such as general red, itch is chapped.
(2) experimental method：
Positive drug and embodiment 1, embodiment 2, embodiment 3 gained Chinese medical extract A, B, C are prepared with DMSO or distilled water,
Used time, basic, normal, high concentration was 25mg/mL, 50mg/mL, 100mg/mL to be diluted containing 10% hyclone culture medium.
Ku812 cell densities are adjusted to 2.2 × 10 with the IMDM culture mediums containing 10% hyclone6Individual/mL cell
Suspension, is inoculated in 96 orifice plates, per the μ L of hole 200.If cell blank control group, model group, positive drug group and test medicine group, often
Group sets 3 parallel holes, 37 DEG C, 5%CO2Incubator culture stands 10min, positive drug and test medicine group add positive drug and by
Reagent thing, final concentration of 50 μ g/mL put pre-temperature in incubator and incubated, after 15min in addition to cell blank group, and each group cell adds thorn
Swashing agent PMA, A12387 makes final concentration be respectively 5nm, 1 μm, the cell blank group addition same volume culture mediums of DMSO containing isoconcentration.2h
Centrifugal separating cell, collects cell supernatant afterwards, determines IL-8, IL-6 content in supernatant.
(3) experimental result
Calculate the basic, normal, high concentration of extract A is respectively to IL-6 inhibiting rates：8.1%th, 13.7%, 28.1%；To IL-8
Inhibiting rate is respectively：5.1%th, 17.7%, 27.0%；The basic, normal, high concentration of extract B is respectively to IL-6 inhibiting rates：9.7%th,
16.4%th, 28.9%；It is respectively to IL-8 inhibiting rates：9.2%th, 18.1%, 30.1%；Extract C is distinguished IL-6 inhibiting rates
For：7.9%th, 20.6%, 23.4%；It is respectively to IL-8 inhibiting rates：6.1%th, 19.9%, 29.8%.Show embodiment 1, reality
Apply example 2, embodiment 3 gained Chinese medical extract A, B, C can by suppress IL-6, IL-8 play alleviate chap, it is Shu Min, antipruritic
Effect.
3rd, the alleviation chap effect of embodiment 4, embodiment 5, the preparation-obtained cream of embodiment 6
Test population：The 50 50-70 Sui volunteer with chapped skin symptom, wherein male 14, women 36.
Amount of samples：Volunteer is chapped after position is soaked 15 minutes with warm water daily and dried, (embodiment 5 is made by cream
It is standby) uniformly it is applied at chap, sooner or later twice daily, use 4 weeks.Cure criterion：Surface skin is flat, no scar, painless
Itch, elasticity is good, skin color is normal.Effectively：Partly there is hyperplastic scar summary close to criterion of cure in treatment face 80%
Surface is higher by, flush is congested, the slightly hard person of matter；It is effective：Treatment face 60% is close to criterion of cure, and part appearance and normal skin are slightly
Difference, is flushed with surrounding skin substantially, and color is slightly red or slightly white；It is invalid：There is obvious hyperplastic scar protuberance in treatment face 80%
Point sheet, it is in irregular shape, it is uneven, or skin is in patch pattern change, flush is congested, matter is hard, obfuscation, there is cusalgia and itch
Sense person.
As a result：1 month cure rate 22% (11), effective percentage is 44% (22), and obvious effective rate is 24% (12), is always had
Efficiency high is up to 90%.
Embodiment 4,6 creams repeat the experimental result be the same as Example 5 of above-mentioned experiment.
4th, the skin barrier repairing effect of cream of the present invention
1st, cuticula moisture loss is tested
Test sample is the gained cream of embodiment 5, and water consumption substitution Chinese medical extract is made blank cream matrix, is used as blank
Control.
Test before through the trained health volunteer 30 of professional (each 15 of men and women), the age at 50~55 years old, institute
There is subject without skin or systemic disease history, recipient site is without exception, and tested period does not smear any unrelated with experiment
Medicine or cosmetics.
Test site room temperature (25 ± 1) DEG C, relative humidity (40 ± 5) %, subject should keep body to be stabilization before test
State.Using 35 DEG C or so clear water wipe subject's both arms after, in start after the 30min that sat quietly under test environment test.
At subject's inboard arm is away from palm base portion 5cm, tested region (4cm × 4cm) is marked, same arm can be simultaneously
Mark multiple regions (interval 1cm), the equal random distribution of test sample.The blank value of each test zone is first measured, then test specimens
By (2.0 ± 0.1) mg/cm2Consumption carry out single application, after coating use Multi-functional skin moisture tester and moisture of skin
Lost instrument measures the moisture loss value in the tested region of 0.5h, 1h, 2h and 4h and blank control region respectively.
Test and the data that 5 times measure in subject's smearing same sample region are averaged, using SPSS statistical softwares
Carry out t check analyses.
2nd, moisture of skin, which scatters and disappears, changes
Moisture of skin windage (Transepidermal water loss, TEWL) is reflected in test period, test block
Domain moisture loss changes with time rule, and it can be with the skin barrier maintenance function of characterization test sample, its smaller, moisture of value
Scatter and disappear fewer, skin repair ability is stronger；Conversely, skin repair ability is weaker.
Percentage of water loss (%)=(sample sets water loss amount-starting moisture windage)/starting moisture windage × 100
Moisture loss result is shown in Fig. 2.It can be seen that from the result：In 4h, the gained cream of embodiment 5 has skin repair energy
Power effect, maintenance most pronounced effects, with skin barrier protective effect, can effectively prevent the generation of chap.Embodiment 4,6 cream
Frost repeats the experimental result be the same as Example 5 of above-mentioned experiment.
5th, skin care cream itching-relieving efficacies clinical verification made from embodiment 5
1st, experimental method：
Patient source is Chinese department of traditional Chinese medicine institute acupuncture hospital dermatology department out-patient.This group totally 36, man 12, female 24
Example, it is the oldest 58 years old, minimum 50 years old.The course of disease is most long 1 year, most short 2 months.Clinic is mainly shown as face, hand, lower limb, foot
Have discomfort during portion's skin, itch, furfur, especially using cosmetics after, there is a series of symptom, it is repeatedly refractory.Only for sensitivity
Crowd of the position based on face, cosmetics can be with user.Exclude severe allergy, systemic anaphylaxis and other there is medical sign
The patient of disease.
2nd, sample application method：
After daily mildy wash face cleaning, the skin care cream for applying embodiment 5 in face and sensitive part is appropriate, once in the morning and once at night.1
Week, 2 weeks, 3 weeks, 4 weeks (1 month) are respectively counted once.
3rd, curative effect determinate standard
(1) cure：Clinical symptom disappearance, condition susceptible disappear；
(2) effectively：Clinical symptom relief, condition susceptible disappear or disappeared, and reduce or reduce；
(3) it is invalid：Clinical symptoms, condition susceptible do not improve.
4th, clinical observation result and conclusion
Use the total situation of the skin care cream clinical observation of 1 month and subject's Symptoms and curative effect made from embodiment 5
Situation refer to table 9.
The total situation of skin care cream clinical observation made from the embodiment 5 of table 9
5th, clinical effectiveness is analyzed
(1) for skin of face sensitive group skin of face cardinal symptom (itch, erythema, dry furfur situation), reality is used
The effective percentage of 1 month of skin care cream made from example 5 is applied up to 94% (34), the cure rate of 1 month is 78% (28).
(2) overall assessment of the trier to the skin care cream of Application Example 5：
A, " feeling very good, be worth application " account for 72% (26)；
B, " feeling fine, can apply " account for 11% (4)；
C, " feel general, can with can be without " account for 14% (5)；
D, " feeling bad, be unworthy applying " account for 3% (1).
(3) as long as adhering to using notable to sensitive skin crowd therapeutic effect.
The cream that inventor is prepared with embodiment 4,6 repeats above-mentioned experiment, as a result ibid.
Claims (10)
1. it is a kind of be used for prevent and alleviate senile skin chap Chinese medicine composition, it is characterised in that described Chinese medicine composition by
Each bulk drug composition of following parts by weight：The parts by weight of teasel root 20~40, bark of eucommia 15-30 parts by weight, the parts by weight of gumbo 15~30 are yellow
Smart 10~20 parts by weight and the parts by weight of the bark of official magnolia 10~20.
2. Chinese medicine composition as claimed in claim 1, it is characterised in that described Chinese medicine composition is each by following parts by weight
Bulk drug is constituted：The parts by weight of teasel root 40, the parts by weight of the bark of eucommia 30, the parts by weight of gumbo 20, the parts by weight of sealwort 15 and the weight of the bark of official magnolia 15
Part.
3. a kind of Chinese medical extract for being used to preventing and alleviating senile skin chap, it is characterised in that the Chinese medical extract is by weighing
Profit requires that the Chinese medicine composition described in 1 or 2 is extracted by the following method and obtained：
(1) after each bulk drug is crushed, each bulk drug is weighed according to the parts by weight described in claim 1 or 2, mixes, obtains each original
Expect the mixture of medicine；
(2) mixture for each bulk drug that step (1) is obtained is carried with concentration of volume percent for 50%~90% (v/v) ethanol
Take, the mass volume ratio of bulk drug mixture and ethanol is 1：10~1：50(g：ML), 55 DEG C~85 DEG C 1~4h of extraction, are obtained
Extract solution；
(3) extract solution for obtaining step (2) is cooled to 20 DEG C~30 DEG C, and 100~200 mesh filter-cloth filterings obtain filtrate；
(4) added in the filtrate obtained to step (3) miscible with ethanol volume identical butanediol in step (2) or propane diols
It is even, the ethanol in solution is rotated out, is filtered by vacuum, filtrate is collected, produces.
4. Chinese medical extract as claimed in claim 3, it is characterised in that the concentration of volume percent of ethanol is in step (2)
The mass volume ratio of 75% (v/v), bulk drug mixture and ethanol is 1：20(g：mL).
5. Chinese medical extract as claimed in claim 3, it is characterised in that the condition of vacuum filtration is in Bu Shi leakages in step (4)
1.2 μm of filter plates are spread in bucket, are filtered by vacuum.
6. purposes of the Chinese medical extract described in claim any one of 3-5 in external-use skin care preparation is prepared, wherein, preferably
, described external-use skin care preparation has effects that Shu Min, antipruritic, prevention and alleviates chapped skin and repair skin barrier.
7. a kind of external-use skin care preparation for being used to preventing and alleviating senile skin chap, it is characterised in that the skin care formulation is by weighing
Profit requires that Chinese medical extract and skin care field customary adjuvant described in any one of 3-5 are made.
8. it is a kind of be used for prevent and alleviate senile skin chap cream, it is characterised in that described cream by A phase, B phase, C phase,
D phases and E phase compositions；
Wherein, A phases by stereth -2, stereth -21, cetostearyl alcohol, Butyrospermum parkii fruit fat extract, vaseline,
Caprylic/capric triglyceride, stearine, stearic acid, lanolin, dimethyl silicone polymer, ethylhexyl palmitate with
And tocopherol acetate composition；
Wherein, B phases are made up of xanthans, glycerine, EDETATE SODIUM, niacinamide, allantoin, glycine betaine and water；
Wherein, C phases are made up of the Chinese medical extract described in α-mannosan aqueous solution and claim any one of 3-5；
Wherein, D phases are made up of emulsifying agent；
Wherein, E phases are made up of preservative；
Wherein, the weight/mass percentage composition of each raw material is as follows：
It is preferred that, the weight/mass percentage composition of each raw material is as follows：
9. a kind of cream for being used to preventing and alleviating senile skin chap as claimed in claim 8, it is characterised in that described
Cream is prepared in accordance with the following methods：
1) B phases：Xanthans is disperseed in advance with glycerine, is added to the water, 70 DEG C -95 DEG C are heated to, 10-60 minutes are incubated；
2) A phases：After A phases raw material is well mixed, 70 DEG C -95 DEG C are heated to, 10-60 minutes are incubated；
3) B phases raw material carries out that A phase raw materials are slowly added into B phases in homogeneous, homogenizing process using 2000-7000r/min,
2000-7000r/min homogeneous 1-10min, add D phase raw materials, 1000-5000r/min homogeneous 1-5 minutes, stirring cooling；
4) C, E phase raw material are added when temperature is down to 30-45 DEG C, stirring is to being completely dissolved；
5) temperature drops to 25-30 DEG C, discharges, ageing；
6) it is filling, packaging, finished product.
10. the cream that senile skin chap is prevented and alleviated to being used for described in claim 8 or 9 is gentle in Shu Min, antipruritic, prevention
Solve the purposes in chapped skin and reparation skin barrier, it is preferred that the applicable crowd of described cream is 50 more than one full year of life
Crowd.
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| CN103301035A (en) * | 2013-05-29 | 2013-09-18 | 美晨集团股份有限公司 | Skin moistening product for preventing skin from being dried and chapped and preparation method of skin moistening product |
| CN104001081A (en) * | 2014-05-29 | 2014-08-27 | 圣原健康产业有限公司 | Anti-chapping traditional Chinese medicine composition and cosmetics containing same |
| CN105381007A (en) * | 2015-11-16 | 2016-03-09 | 北京工商大学 | Externally-applied traditional Chinese medicine composition with effect on whitening skin, preparation as well as preparation method and application thereof |
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| CN103301035A (en) * | 2013-05-29 | 2013-09-18 | 美晨集团股份有限公司 | Skin moistening product for preventing skin from being dried and chapped and preparation method of skin moistening product |
| CN104001081A (en) * | 2014-05-29 | 2014-08-27 | 圣原健康产业有限公司 | Anti-chapping traditional Chinese medicine composition and cosmetics containing same |
| CN105381007A (en) * | 2015-11-16 | 2016-03-09 | 北京工商大学 | Externally-applied traditional Chinese medicine composition with effect on whitening skin, preparation as well as preparation method and application thereof |
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Application publication date: 20170825 |