CN107080777A - A kind of new citrus rag extract and preparation method thereof and the medical usage relaxed bowel - Google Patents
A kind of new citrus rag extract and preparation method thereof and the medical usage relaxed bowel Download PDFInfo
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- CN107080777A CN107080777A CN201710212909.7A CN201710212909A CN107080777A CN 107080777 A CN107080777 A CN 107080777A CN 201710212909 A CN201710212909 A CN 201710212909A CN 107080777 A CN107080777 A CN 107080777A
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- extract
- pectin
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- citrus rag
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- 239000000284 extract Substances 0.000 title claims abstract description 57
- 235000020971 citrus fruits Nutrition 0.000 title claims abstract description 43
- 241000207199 Citrus Species 0.000 title claims abstract description 42
- 238000000605 extraction Methods 0.000 title claims description 24
- 238000002360 preparation method Methods 0.000 title abstract description 7
- 235000010987 pectin Nutrition 0.000 claims abstract description 66
- 239000001814 pectin Substances 0.000 claims abstract description 66
- 229920001277 pectin Polymers 0.000 claims abstract description 66
- 150000003384 small molecules Chemical class 0.000 claims abstract description 38
- ULSUXBXHSYSGDT-UHFFFAOYSA-N tangeretin Chemical compound C1=CC(OC)=CC=C1C1=CC(=O)C2=C(OC)C(OC)=C(OC)C(OC)=C2O1 ULSUXBXHSYSGDT-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000000926 separation method Methods 0.000 claims abstract description 11
- 235000008603 tangeritin Nutrition 0.000 claims abstract description 11
- 239000003814 drug Substances 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims description 25
- 239000007788 liquid Substances 0.000 claims description 20
- 238000000108 ultra-filtration Methods 0.000 claims description 17
- 229930003944 flavone Natural products 0.000 claims description 11
- 235000011949 flavones Nutrition 0.000 claims description 11
- 239000000463 material Substances 0.000 claims description 10
- 150000002213 flavones Chemical class 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 8
- 239000012528 membrane Substances 0.000 claims description 7
- 238000012545 processing Methods 0.000 claims description 7
- 239000000706 filtrate Substances 0.000 claims description 6
- 239000000047 product Substances 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 5
- 230000036541 health Effects 0.000 claims description 4
- 238000001728 nano-filtration Methods 0.000 claims description 4
- 238000011084 recovery Methods 0.000 claims description 4
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 150000002212 flavone derivatives Chemical class 0.000 claims description 3
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 claims description 3
- 230000033228 biological regulation Effects 0.000 claims description 2
- 239000012535 impurity Substances 0.000 claims description 2
- 238000009413 insulation Methods 0.000 claims description 2
- 239000010178 pectin extract Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 15
- 230000002040 relaxant effect Effects 0.000 abstract description 3
- 239000002131 composite material Substances 0.000 abstract description 2
- 206010010774 Constipation Diseases 0.000 description 16
- 239000000976 ink Substances 0.000 description 15
- 241000699666 Mus <mouse, genus> Species 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 230000004048 modification Effects 0.000 description 10
- 238000012986 modification Methods 0.000 description 10
- 239000013642 negative control Substances 0.000 description 9
- 239000000126 substance Substances 0.000 description 8
- PYMYPHUHKUWMLA-UHFFFAOYSA-N 2,3,4,5-tetrahydroxypentanal Chemical compound OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 7
- 229940040387 citrus pectin Drugs 0.000 description 7
- 239000009194 citrus pectin Substances 0.000 description 7
- 238000003304 gavage Methods 0.000 description 7
- AXDJCCTWPBKUKL-UHFFFAOYSA-N 4-[(4-aminophenyl)-(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]aniline;hydron;chloride Chemical compound Cl.C1=CC(=N)C(C)=CC1=C(C=1C=CC(N)=CC=1)C1=CC=C(N)C=C1 AXDJCCTWPBKUKL-UHFFFAOYSA-N 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 6
- 230000013872 defecation Effects 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 239000012153 distilled water Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 239000003292 glue Substances 0.000 description 5
- 230000000968 intestinal effect Effects 0.000 description 5
- 230000037396 body weight Effects 0.000 description 4
- 150000004676 glycans Chemical class 0.000 description 4
- 150000008442 polyphenolic compounds Chemical class 0.000 description 4
- 235000013824 polyphenols Nutrition 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 239000005017 polysaccharide Substances 0.000 description 4
- 210000000813 small intestine Anatomy 0.000 description 4
- 241001672694 Citrus reticulata Species 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 229930003935 flavonoid Natural products 0.000 description 3
- 150000002215 flavonoids Chemical class 0.000 description 3
- 235000017173 flavonoids Nutrition 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 230000008991 intestinal motility Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
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- 241000894006 Bacteria Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- -1 compound diphenoxylate Chemical class 0.000 description 2
- 238000004132 cross linking Methods 0.000 description 2
- 229960004192 diphenoxylate Drugs 0.000 description 2
- 230000009144 enzymatic modification Effects 0.000 description 2
- 230000032050 esterification Effects 0.000 description 2
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- 150000002576 ketones Chemical class 0.000 description 2
- 230000008855 peristalsis Effects 0.000 description 2
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- 238000012360 testing method Methods 0.000 description 2
- UOQHWNPVNXSDDO-UHFFFAOYSA-N 3-bromoimidazo[1,2-a]pyridine-6-carbonitrile Chemical compound C1=CC(C#N)=CN2C(Br)=CN=C21 UOQHWNPVNXSDDO-UHFFFAOYSA-N 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000010428 Muscle Weakness Diseases 0.000 description 1
- 206010028372 Muscular weakness Diseases 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
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- 239000003963 antioxidant agent Substances 0.000 description 1
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- 231100000357 carcinogen Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
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- 238000001727 in vivo Methods 0.000 description 1
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- 230000031891 intestinal absorption Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 229940099563 lactobionic acid Drugs 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 210000001187 pylorus Anatomy 0.000 description 1
- 210000000664 rectum Anatomy 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 235000021391 short chain fatty acids Nutrition 0.000 description 1
- 150000004666 short chain fatty acids Chemical class 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/732—Pectin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to field of medicaments, more particularly to a kind of new citrus rag extract and its preparation method and application.A kind of new citrus rag extract, the extract includes citrus rag and extracts small molecule pectin and tangeritin that the extract after high molecular pectin passes through physical separation preparation, described extract is per 100ml pectin containing small molecule >=2000mg, tangeritin >=400mg.The citrus rag extract small molecule pectin and tangeritin of the present invention, which belongs to natural composite parts, good effect to relaxing bowel.
Description
Technical field
The present invention relates to field of medicaments, more particularly to a kind of new citrus rag extract and its preparation method and application.
Background technology
Citrus pectin is derived from the polysaccharide of plant cell wall, may generally serve as food additives and uses, and has good glue
It is solidifying, stably, emulsification, thickening, suspension function, can not be by likewise, natural citrus pectin molecule amount is big (Da of 5-30 ten thousand), after eating
Intestinal absorption and it can not be made to play one's part to the full in vivo.In consideration of it, carrying out the modification of size and structure to its molecule, have
Strengthen the prospect of biological function.
A few class main methods that pectin is modified, including chemical (pH) modification, enzyme modification, heat modification, radiation modification, grafting change
Property, cross-linking modified and substituting modification；
At present, small molecule pectin extraction technology both domestic and external, mainly uses natural citrus pectin to pass through method of modifying system
It is standby, including chemical (pH) modification, enzyme modification, heat modification, radiation modification, graft modification, cross-linking modified and substituting modification；These sides
Method carries out the processing of natural pectin strand chain rupture, can obtain the small molecule pectin that molecular weight is 2000-35000Da, esterification degree
2-30%, the effect one of hydrolysis is that, by the cut-out of pectin straight chain to reduce molecular weight, two be reduction esterification degree, the process of straight chain cut-out
The technique of middle use, can cause the activity reduction of pectin, and the effect obtained after taking only has 10% or so.
Past, because the only a few enterprise that small molecule pectin only has American-European, Japan and other countries grasps core production technology, I
State's small molecule pectin industry is limited to foreign technology monopolization for a long time, and the product overwhelming majority is based on import, so in the urgent need to certainly
Main research and development, the domestic small molecule pectin of technological precedence enrich domestic market, and we can make full use of the mandarin orange of China's abundant completely
Tangerine resource, produces the small molecule citrus pectin of high-quality, meets the domestic growing market demand, can suffer from the limit of technology
System so that this market is captured by overseas enterprise substantially.
Applicant Fan Xiao green grass or young crops applied Chinese invention patent (CN200610050803.3, CN200610051667.X,
CN201010226113.5, CN201110028976.6) disclose the new application of low molecular citrus pectin, low molecular citrus fruit
Glue is extracted after pectin using sodium hydroxide and potassium hydroxide catalysed acquisition low molecular citrus pectin by dry orange peel.It is used fruit
Glue straight chain is cut off in the way of reducing molecular weight, can cause the activity reduction of pectin.
Chinese invention patent application (the application number CN201610531658.4, public affairs of the high seapeak application of the present inventor
Open day：2016.12.07 a kind of method that pectin and polyphenol are synchronously extracted in citrus can processing technique) is disclosed, is passed through respectively
The separator of different stage isolate high molecular pectin, low molecule pectin and polyphenol in citrus can processing process water,
The materials such as monose, it is to avoid process water is difficult in traditional can processing causes environmental pollution and physical waste, and each is different
The separation and Extraction mode that the separation of Middle Molecular Substance is used is also all different, such as ultrafiltration, nanofiltration etc. will not make recovery process too
It is complicated.Present invention also offers the system that pectin and polyphenol are extracted in a kind of citrus can process water, according to high molecular pectin, low
Molecule pectin and polyphenol, monosaccharide molecule amount are of different sizes, the separator for being used to filter using different film devices, promote point
The above-mentioned substance purity separated out is higher, and the heavy metal and salt in process water can be effectively removed comprising demineralizer in system
Class, it is to avoid pollutions of these materials to the high molecular pectin after extraction, low molecule pectin and phenol, monose.
Constipation is one of clinically common symptom.With the quickening pace of modern life, the operating pressure of people is increasing,
Plus the irregular change with dietary structure of living, processed refined low fiber food is on the increase, and result in intestines peristalsis
, there is constipation in decrease, rectum muscle weakness etc..The carcinogen that long-term constipation can make enteric bacterial fermentation and produce stimulates intestines
Mucosal epithelial cells, cause special-shaped hyperplasia, easily induce canceration.
The content of the invention
First purpose of the present invention is to provide in a kind of new citrus rag extract, the citrus rag extract small point
Sub- pectin is a kind of water-soluble polysaccharide directly extracted from citrus rag, maintains the activity of pectin, tangeritin be from
The general name of the Flavonoid substances extracted is directly separated in citrus rag.Second object of the present invention is to provide a kind of above-mentioned
The preparation method of citrus rag extract, third object of the present invention is to provide the pharmaceutical of above-mentioned citrus rag extract
On the way.
In order to realize first above-mentioned purpose, present invention employs following technical scheme：
A kind of new citrus rag extract, the extract includes citrus rag and extracts the extract warp after high molecular pectin
Cross the small molecule pectin and tangeritin of physical separation preparation, described extract per 100ml pectin containing small molecule >=2000mg,
Tangeritin >=400mg.
Preferably, the molal weight of described small molecule pectin is below 50000Da, most preferably described small molecule fruit
Between the molal weight 1000-50000Da of glue.
In order to realize second above-mentioned purpose, present invention employs following technical scheme：
A kind of method for preparing described citrus rag extract, this method comprises the following steps：
1) processing of raw material
By citrus rag raw material, tank is carried into acid, and is heated to 40-50 DEG C, PH to 3.5-4.5 is adjusted, insulation 3~5 is small
When；
2) filter
Liquid after extraction, through filtering out residue, takes filtrate to enter extractor, filter residue carries out secondary extraction；
3) extract
Liquid after extraction enters extractor, adds extract, carries out high molecular pectin extraction, and the extract after extraction enters
Enter pressing device, will secure satisfactory grades sub- pectin；
4) extract is reclaimed
Extract is reclaimed by extraction recovery device, and extract residual fraction is the material liquid of small molecule pectin and flavones；
5) ultrafiltration I
Raw material containing small molecule pectin and flavones filters out the pectin and impurity more than 50,000 molecular weight by ultrafiltration；
6) ultrafiltration II
The filtrate after ultra-filtration and separation and the filter of ultrafiltration concentration I from 1000 molecular weight, obtains small molecule liquid pectin；
7) nanofiltration
From NF membrane separating flavone liquid, concentrated after ultrafiltration II is separated through filter into NF membrane, obtain citrus
Flavones liquid.
Certainly, small molecule pectin of the invention can also be special using the Chinese invention of the high seapeak application of the present inventor
Profit application (application number CN201610531658.4, publication date：2016.12.07) open method acquisition.
In order to realize the 3rd above-mentioned purpose, present invention employs following technical scheme：
Citrus rag extract is used to prepare the application in medicine, health products or the food relaxed bowel.
The present invention there is further disclosed herein extract of the citrus rag after high molecular pectin is extracted and be prepared by physical separation
Small molecule pectin be used to prepare the application in the medicine, health products or the food that relax bowel.Preferably, described small molecule
The molal weight of pectin is between below 50000Da, the molal weight 1000-50000Da of most preferably described small molecule pectin.
Citrus rag extract small molecular pectin of the present invention is a kind of water solubility directly extracted from citrus rag
Polysaccharide, small molecule citrus pectin prepared by this method, molal weight is in 1000-50000 dalton, exclusive world-class UF membrane
Production technology, the fine activity for maintaining pectin.Its characteristic for being different from domestic and international market modified small molecule pectin mainly has：
Pure natural, no added, high activity, i.e., do not destroy the chemical constitution activity of pectin itself.
Small molecule pectin of the present invention derives from plant cell wall, be it is a kind of connected by α-Isosorbide-5-Nitrae glycosidic bond poly- half
The long chain polysaccharides polymer of lactobionic acid, is a kind of pure natural dietary fiber, it is impossible to divided by the enzyme in the digested liquid of human body
Solution, partly by microbial fermentation can be decomposed into short chain fatty acids in big enteral, so as to reduce gut pH, suppress harmful intestinal tract bacteria
Growth, promotes beneficial bacterium propagation, strengthens intestinal peristalsis function.The moisture of 2.5 times of volume itself is also can absorb simultaneously, becomes excrement
It is soft and be easy to discharge, the sorrow of constipation is released, effect of ejecting poison is quickly notable.Tangeritin is that extraction is directly separated from citrus rag
The general name of the Flavonoid substances gone out, Flavonoid substances have anti-oxidant, antithrombotic, antitumor, anti-inflammation, antiviral and toxin expelling
Etc. a variety of effects, there is adjustment effect to metabolism.Citrus rag extract small molecule pectin and the tangeritin category of the present invention
There is good effect to relaxing bowel in natural composite parts.
Brief description of the drawings
Fig. 1 is the process chart of the embodiment of the present invention 1.
Embodiment
Embodiment 1
A kind of method for preparing citrus rag extract as shown in Figure 1, this method comprises the following steps：
1) processing of raw material
Citrus rag carries tank after crushing, cleaning, dry into acid, and is heated to 40-50 DEG C, with sour (HCl) regulation
PH to 3.5-4.5, is incubated 4 hours.
2) filter
Liquid after extraction, through filtering out residue, takes filtrate to enter extractor, filter residue carries out secondary extraction.
3) extract
Liquid after extraction enters extractor, adds extract, carries out high molecular pectin extraction, and the extract after extraction enters
Enter pressing device, will secure satisfactory grades sub- pectin.
4) extract is reclaimed
Extract is reclaimed by extraction recovery device, and extract residual fraction is the material liquid of small molecule pectin and flavones.
5) ultrafiltration I
Raw material containing small molecule pectin and flavones filters out the pectin and magazine more than 50,000 molecular weight by ultrafiltration.
6) ultrafiltration II
From the filtrate risen after filter separation and the filter of ultrafiltration concentration I of 1000 molecular weight, 1000-5 ten thousand small molecule fruit is obtained
Glue.
7) nanofiltration
From NF membrane separating flavone liquid, passing through after filter is separated into NF membrane for filter II will be played and concentrated, citrus is obtained
Flavones liquid.
Embodiment 2
1.1 material
Embodiment 1 is obtained, and finished product is brown suspension.By determining, per 100ml pectin containing small molecule >=2000mg, mandarin orange
Orange ketone (VP) >=400mg, total solid >=4500mg.
1.2 animals and packet
Cleaning grade (II grade) Kunming kind male white mouse 150, body weight is 22~25g, is tested by Military Medical Science Institute
Animal center is provided.If the 0.2nd, 0.4,0.8ml/ (25gbw) (be respectively equivalent to human body recommended amounts 5,10,20 times) three agent
The experimental group of amount, while Constipation Model control group and negative control group are set, every group of 15 animals.
1.3 method
According to《Health food is examined and assessment technique specification》" the bowel relaxing functions method of inspection " is carried out in (version in 2003), tool
Body method is as follows：
1.3.1 intestinal motility is tested
Each experimental group presses 0.8 milliliter/orally administration tested material, and negative and model control group gives the distilled water of equivalent,
Daily gavage 1 time, continuous 7 days.Water 16h is can't help in that afternoon of the last to sample, each group animal fasting, gives model the next morning
(Kang Pu medicine company pharmacy responsibility in Changzhou is public for the compound diphenoxylate solution that control group and three experimental group dosage are 5mg/ (kgbw)
Department's production, lot number：1604014)【0.2ml/(10g·bw)】, negative control group gives equivalent distilled water.Wait after 30min, three
Individual dosage group gives the prepared Chinese ink containing corresponding tested material and (takes india ink 1ml, add 49ml distilled water and be configured to 2% prepared Chinese ink, gavage
Dosage is 0.1ml/10gbw.Blent with prepared Chinese ink with gavage liquid, gavage total amount keeps 1ml), model control group and negative control
Group gives 2% prepared Chinese ink.Give cervical dislocation after prepared Chinese ink 25min and put to death animal, open abdominal cavity separation mesenterium, clip upper end is certainly deep and remote
Door, the intestinal tube of lower end to ileocecus, are placed on a white plastic paper greatly, small intestine gently are pulled into straight line and measured.Intestinal tube is long
Spend for " total small intestinal length ", be " prepared Chinese ink propulsion length " from pylorus to prepared Chinese ink forward position.Ink progradation is calculated by following equation
(P), and statistical analysis again after data conversion (Х) is carried out.
Ink progradation (P)=(prepared Chinese ink promotes length/total small intestinal length) × 100%；Х=sin √ ￣ P.
1.3.2 defecation is tested
Tested to tested material method with intestinal motility.Water 16h is can't help in that afternoon of the last to sample, each group animal fasting,
Give the compound diphenoxylate solution that model control group and three experimental group dosage are 10mg/ (kgbw) the next morning
【0.2ml/(10g·bw)】, negative control group gives equivalent distilled water.Wait after 30min, three dosage groups give containing it is corresponding by
The fuchsin solution for trying thing (takes fuchsin powder 1g, adds 49ml distilled water and be configured to 2% fuchsin, given low is 0.1ml/10g
bw.Mixed liquor is blent into fuchsin and gavage liquid, gavage total amount keeps 1ml), model control group and negative control group give 2%
Fuchsin, starts simultaneously at timing.Since gavage fuchsin, every animal of observed and recorded arrange first red toilet take time, 6h defecations grain
Count and weigh.
1.3.3 statistical procedures
Variance analysis is carried out using SPSS softwares and examined two-by-two.
2 results
Influence of the 2.1 citrus rag extracts to mouse weight
From table 1,2 results, body weight of each dosage group mouse before and after experiment and negative control group and model control group
Compare, there are no significant difference (P>0.05), show that the extract increases mouse weight not making significant difference.
Body weight compares before and after the mouse small intestine exercise testing of table 1
Note:Comparing difference there are no significant meaning (P between each group in table>0.05).
Body weight compares before and after the experiment of the mouse defecation of table 2
Note:Comparing difference there are no significant meaning (P between each group in table>0.05).
The influence that 2.2 citrus rag extracts are moved to mouse small intestine
From table 3, the ink progradation of Constipation Model control group mice is substantially less than negative control group (P<0.05), table
Show that Constipation Model is successfully established.Low, middle dose group mouse ink progradation is higher than model control group (P<0.05), turn through data
Change rear statistical analysis, 5 times, 10 multiple dose groups there is significant difference (P compared with Constipation Model group<0.05), wherein 10 times of groups
Effect is better than 5 times of groups, shows that the extract has the effect for the intestinal motility for promoting mouse.
The influence that the citrus rag extract of table 3 is moved to mouse small intestine
Note:1.aP<0.05, representing that each group ink progradation is compared with Constipation Model control group has significant difference；
2.bP<0.05, representing that each group ink progradation conversion value Х is compared with Constipation Model control group has significant difference.
Influence of the 2.3 citrus rag extracts to mouse defecation time, stool interval and feces volume
From table 4, just the time is more than negative control group (P to the small mouse's head of Constipation Model control group<0.01), and excrement
Grain number is less than negative control group (P<0.01).The first just time of each dosage group has shortened than Constipation Model control group, wherein 5
Again, 10 multiple dose group differences compared with model control group have pole significant (P<0.01), 20 times of groups are poor compared with model group
It is different to have significant (P<0.05)；Each dosage group fecal grains are more than Constipation Model control group, wherein 5 times, 10 times of groups and mould
Type control group difference has pole significant (P<0.01), 20 times of group differences compared with model group have significant (P<
0.05)；Each dosage group stool weight is all higher than Constipation Model control group, and has pole significant difference (P<0.01).Result above
Show, three dosage group given the test agent can substantially shorten the first just time, and increase its defecation quantity and weight of mice with constipation.
Influence of the tested material of table 4 to mouse defecation time, stool interval and feces volume
Note:Compared with Constipation Model control group, * P ＜ 0.05, * * P ＜ 0.01；Δ P ＜ 0.05, Δ Δ P ＜ 0.01；#P ＜
0.05, ##P ＜ 0.01.
Claims (8)
1. a kind of new citrus rag extract, it is characterised in that：The extract includes citrus rag and is extracting high molecular pectin
Extract afterwards passes through small molecule pectin and tangeritin prepared by physical separation, and described extract contains small molecule per 100ml
Pectin >=2000mg, tangeritin >=400mg.
2. a kind of new citrus rag extract according to claim 1, it is characterised in that：Described small molecule pectin
Molal weight is below 50000Da.
3. a kind of new citrus rag extract according to claim 2, it is characterised in that：Described small molecule pectin
Molal weight is between 1000-50000Da.
4. a kind of method of the citrus rag extract prepared described in claim 1 or 2, it is characterised in that this method includes following
The step of：
1) processing of raw material
By citrus rag raw material, tank is carried into acid, and be heated to 40-50 DEG C, regulation PH to 3.5-4.5, insulation 3~5 hours；
2) filter
Liquid after extraction, through filtering out residue, takes filtrate to enter extractor, filter residue carries out secondary extraction；
3) extract
Liquid after extraction enters extractor, adds extract, carries out high molecular pectin extraction, and the extract after extraction enters pressure
Device is squeezed, will secure satisfactory grades sub- pectin；
4) extract is reclaimed
Extract is reclaimed by extraction recovery device, and extract residual fraction is the material liquid of small molecule pectin and flavones；
5) ultrafiltration I
Raw material containing small molecule pectin and flavones filters out the pectin and impurity more than 50,000 molecular weight by ultrafiltration；
6) ultrafiltration II
The filtrate after ultra-filtration and separation and the filter of ultrafiltration concentration I from 1000 molecular weight, obtains small molecule liquid pectin；
7) nanofiltration
From NF membrane separating flavone liquid, concentrated after ultrafiltration II is separated through filter into NF membrane, obtain tangeritin
Liquid.
5. the citrus rag extract described in claim 1 or 2 or 3 is used to prepare medicine, health products or the food relaxed bowel
In application.
6. the small molecule pectin that extract of the citrus rag after high molecular pectin is extracted is prepared by physical separation is used to prepare
Application in the medicine, health products or the food that relax bowel.
7. application according to claim 6, it is characterised in that the molal weight of described small molecule pectin be 50000Da with
Under.
8. application according to claim 6, it is characterised in that the molal weight of described small molecule pectin is 1000-
Between 50000Da.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108310095A (en) * | 2018-02-12 | 2018-07-24 | 杭州络通生物科技有限公司 | Citrus rag extract is used to prepare the application in the drug, health products or food for treating or preventing essential hypertension |
| CN108379372A (en) * | 2018-02-12 | 2018-08-10 | 杭州络通生物科技有限公司 | Citrus rag extract is used to prepare the application in the drug, health products or food for treating or preventing type-2 diabetes mellitus |
| CN109043540A (en) * | 2018-10-16 | 2018-12-21 | 中国计量大学 | Improve method, flavone composition and the product containing chromocor extract of chromocor extract biology accessibility |
| CN111227247A (en) * | 2020-03-10 | 2020-06-05 | 中国农业科学院农产品加工研究所 | Citrus product with functions of improving intestinal flora structure and relieving atherosclerosis as well as preparation method and application of citrus product |
| CN112121731A (en) * | 2020-10-15 | 2020-12-25 | 崔万哲 | Washing article and preparation method thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115919941A (en) * | 2023-01-06 | 2023-04-07 | 杭州络通生物科技有限公司 | Medicine for regulating intestinal flora by using citrus reticulata blanco extract as main component and preparation method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106188335B (en) * | 2016-06-29 | 2019-02-15 | 高海峰 | The synchronous method and system for extracting pectin and polyphenol in citrus can processing technique |
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2017
- 2017-04-01 CN CN201710212909.7A patent/CN107080777A/en active Pending
- 2017-12-11 CN CN201711304483.4A patent/CN108042631A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108310095A (en) * | 2018-02-12 | 2018-07-24 | 杭州络通生物科技有限公司 | Citrus rag extract is used to prepare the application in the drug, health products or food for treating or preventing essential hypertension |
| CN108379372A (en) * | 2018-02-12 | 2018-08-10 | 杭州络通生物科技有限公司 | Citrus rag extract is used to prepare the application in the drug, health products or food for treating or preventing type-2 diabetes mellitus |
| CN109043540A (en) * | 2018-10-16 | 2018-12-21 | 中国计量大学 | Improve method, flavone composition and the product containing chromocor extract of chromocor extract biology accessibility |
| CN111227247A (en) * | 2020-03-10 | 2020-06-05 | 中国农业科学院农产品加工研究所 | Citrus product with functions of improving intestinal flora structure and relieving atherosclerosis as well as preparation method and application of citrus product |
| CN112121731A (en) * | 2020-10-15 | 2020-12-25 | 崔万哲 | Washing article and preparation method thereof |
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| CN108042631A (en) | 2018-05-18 |
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