CN107075559A - 临床微量活检石蜡包埋组织的前处理方法 - Google Patents

临床微量活检石蜡包埋组织的前处理方法 Download PDF

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Publication number
CN107075559A
CN107075559A CN201480081410.1A CN201480081410A CN107075559A CN 107075559 A CN107075559 A CN 107075559A CN 201480081410 A CN201480081410 A CN 201480081410A CN 107075559 A CN107075559 A CN 107075559A
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soaking
tissue
section
tumor tissue
genomic dna
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崔路漫
赵鑫
侯勇
吴逵
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BGI Shenzhen Co Ltd
BGI Genomics Co Ltd
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BGI Shenzhen Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

本发明公开了一种临床微量活检石蜡包埋组织的前处理方法。该方法包括如下步骤：1)将经石蜡包埋的临床活检组织蜡块进行连续切片；2)取同一例临床活检组织蜡块连续切片中的一张切片，进行HE染色，得到对照切片；3)从所述同一例临床活检组织蜡块连续切片中取出未经HE染色的切片，脱蜡后作为待分离组织切片；4)根据所述对照切片的HE染色结果，从所述待分离组织切片中将肿瘤组织切割下来。

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PCT国内申请，说明书已公开。

Claims (16)

  1. PCT国内申请，权利要求书已公开。
CN201480081410.1A 2014-09-29 2014-09-29 临床微量活检石蜡包埋组织的前处理方法 Pending CN107075559A (zh)

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PCT/CN2014/000886 WO2016004548A1 (zh) 2014-09-29 2014-09-29 临床微量活检石蜡包埋组织的前处理方法

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CN107075559A true CN107075559A (zh) 2017-08-18

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108181148A (zh) * 2017-12-25 2018-06-19 天津市海河医院 Ct引导下经皮肺穿刺活检的组织压片及现场评价方法
CN111088216A (zh) * 2019-12-16 2020-05-01 齐妍 一种用于石蜡包埋组织分离检测细胞的方法
CN114689407A (zh) * 2022-04-14 2022-07-01 四川大学华西医院 一种动物微小组织石蜡切片的制作方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3559271A1 (en) * 2016-12-22 2019-10-30 Ventana Medical Systems, Inc. Fully automated nucleic acid extraction method for tissue samples

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103602726A (zh) * 2013-10-21 2014-02-26 田埂 同时对多种核酸样本进行测序的方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103602726A (zh) * 2013-10-21 2014-02-26 田埂 同时对多种核酸样本进行测序的方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
杜斌等: "《病理学实验与学习指导》", 31 January 2011 *
黄坚等: ""NBS1基因SNPs与中国汉族人群原发性肝癌的遗传易感性"", 《生物化学与生物物理进展》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108181148A (zh) * 2017-12-25 2018-06-19 天津市海河医院 Ct引导下经皮肺穿刺活检的组织压片及现场评价方法
CN111088216A (zh) * 2019-12-16 2020-05-01 齐妍 一种用于石蜡包埋组织分离检测细胞的方法
CN114689407A (zh) * 2022-04-14 2022-07-01 四川大学华西医院 一种动物微小组织石蜡切片的制作方法
CN114689407B (zh) * 2022-04-14 2023-05-19 四川大学华西医院 一种动物微小组织石蜡切片的制作方法

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