CN107075539B - 用于分析结构不同的复杂脂质的方法、组合物和试剂盒 - Google Patents

用于分析结构不同的复杂脂质的方法、组合物和试剂盒 Download PDF

Info

Publication number
CN107075539B
CN107075539B CN201580040519.5A CN201580040519A CN107075539B CN 107075539 B CN107075539 B CN 107075539B CN 201580040519 A CN201580040519 A CN 201580040519A CN 107075539 B CN107075539 B CN 107075539B
Authority
CN
China
Prior art keywords
lipid
site
mixture
fatty acids
fatty acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201580040519.5A
Other languages
English (en)
Chinese (zh)
Other versions
CN107075539A (zh
Inventor
史蒂文·沃特金斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Metabolon Inc
Original Assignee
Metabolon Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metabolon Inc filed Critical Metabolon Inc
Publication of CN107075539A publication Critical patent/CN107075539A/zh
Application granted granted Critical
Publication of CN107075539B publication Critical patent/CN107075539B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N2030/042—Standards
    • G01N2030/045—Standards internal
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2405/00—Assays, e.g. immunoassays or enzyme assays, involving lipids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2496/00—Reference solutions for assays of biological material
    • G01N2496/80—Multi-analyte reference solutions containing cholesterol, glucose and the like
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/104165—Lipid, cholesterol, or triglyceride standard or control

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201580040519.5A 2014-07-30 2015-07-30 用于分析结构不同的复杂脂质的方法、组合物和试剂盒 Active CN107075539B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462031099P 2014-07-30 2014-07-30
US62/031099 2014-07-30
PCT/US2015/042904 WO2016019140A1 (en) 2014-07-30 2015-07-30 Methods, compositions, and kits for analysis of structurally diverse complex lipids

Publications (2)

Publication Number Publication Date
CN107075539A CN107075539A (zh) 2017-08-18
CN107075539B true CN107075539B (zh) 2021-11-12

Family

ID=55218314

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201580040519.5A Active CN107075539B (zh) 2014-07-30 2015-07-30 用于分析结构不同的复杂脂质的方法、组合物和试剂盒

Country Status (8)

Country Link
US (1) US11181535B2 (enExample)
EP (1) EP3174991B1 (enExample)
JP (1) JP6810023B2 (enExample)
CN (1) CN107075539B (enExample)
AU (1) AU2015296304B2 (enExample)
CA (1) CA2955204A1 (enExample)
ES (1) ES2978375T3 (enExample)
WO (1) WO2016019140A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3602605A4 (en) * 2017-03-31 2020-12-30 Metabolon, Inc. COMPREHENSIVE AND QUANTITATIVE LIPID AND TOCOPHEROL ANALYSIS
JP7306676B2 (ja) * 2019-02-26 2023-07-11 国立大学法人九州大学 脂質プロファイリングシステム、脂質プロファイリング方法、及び脂質プロファイリングプログラム
CN114144679A (zh) * 2019-06-04 2022-03-04 阿凡提极性脂质有限责任公司 用于脂质组学中的通用脂质定量标准
CN110609097A (zh) * 2019-09-12 2019-12-24 谱尼测试集团股份有限公司 一种磷脂酰丝氨酸类化合物的筛查方法
CN116106283B (zh) * 2023-02-16 2025-07-29 清华大学 缩醛磷脂的荧光分析方法与应用
CN116879415A (zh) * 2023-05-25 2023-10-13 厦门市迈理奥科技有限公司 一种脂质组学分析内标及其用途和检测方法
EP4741813A1 (en) * 2023-07-03 2026-05-13 Shimadzu Corporation Analysis method using lysophosphatidylcholine compound containing stable isotope element in choline moiety

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1952650A (zh) * 2006-08-31 2007-04-25 华南理工大学 一种定量测定纸浆树脂中甘油三酯含量的方法
CN102105792A (zh) * 2008-05-28 2011-06-22 巴斯夫欧洲公司 评估肝毒性的工具和方法
WO2014016586A1 (en) * 2012-07-24 2014-01-30 University Of Dundee Isotopic labelling of proteins
CN103743851A (zh) * 2014-02-14 2014-04-23 中国农业科学院油料作物研究所 一种食用油中甘油三酯的单柱二维液相色谱-质谱分析方法及其应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4224031A (en) * 1977-11-15 1980-09-23 Mee John M L CI Mass spectrometric analysis of physiologically active compounds
GB0128498D0 (en) 2001-11-28 2002-01-23 Inst Of Child Health International standards for sphingolipids
US20040024065A1 (en) 2002-03-11 2004-02-05 Watkins Steven M. Novel metabolic targets and markers
US7306952B2 (en) * 2002-06-26 2007-12-11 Washington University In St. Louis Molecular fingerprinting of triglycerides in biological samples by electrospray ionization tandem mass spectrometry
AU2004256368B2 (en) * 2003-07-11 2010-08-05 Tatiana A. Egorova-Zachernyuk Compositions and methods for stable isotope labelling of biological compounds
US20100159540A1 (en) * 2007-03-26 2010-06-24 Ana Rodriguez Synthesis of resolvins and intermediates, compounds prepared thereby, and uses thereof
US8263413B1 (en) * 2008-10-17 2012-09-11 Andrew S Hansen Methods for analyzing lipids and membrane proteins in biological matter using stable isotopes and mass spectrometry
SG10201405975VA (en) * 2009-10-01 2014-11-27 Phenomenome Discoveries Inc Serum-based biomarkers of pancreatic cancer and uses thereof for diseasedetection and diagnosis
EP2345897A1 (en) * 2010-01-18 2011-07-20 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Improved method for the detection of polyunsaturated fatty acids
CN101824064A (zh) * 2010-01-29 2010-09-08 上海化工研究院 一种13c标记胆甾醇羧酸酯的合成方法
US10705100B1 (en) * 2013-09-11 2020-07-07 HB Biotech, LLC Methods for analyzing lipids and membrane proteins in biological matter using stable isotopes and mass spectrometry

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1952650A (zh) * 2006-08-31 2007-04-25 华南理工大学 一种定量测定纸浆树脂中甘油三酯含量的方法
CN102105792A (zh) * 2008-05-28 2011-06-22 巴斯夫欧洲公司 评估肝毒性的工具和方法
WO2014016586A1 (en) * 2012-07-24 2014-01-30 University Of Dundee Isotopic labelling of proteins
CN103743851A (zh) * 2014-02-14 2014-04-23 中国农业科学院油料作物研究所 一种食用油中甘油三酯的单柱二维液相色谱-质谱分析方法及其应用

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Cholesterol efflux analyses using stable isotopes and mass spectrometry;Robert J. Brown等;《Analytical Biochemistry》;20121013;第433卷;第56-64页 *
Plasma lipid profi ling in a large population-based cohort;Jacquelyn M. Weir;《Journal of Lipid Research》;20130718;第54卷;第2898-2980页 *
Quantitation and Standardization of Lipid Internal Standards for Mass Spectroscopy;Jeff D. Moore等;《Methods in Enzymology》;20071022;第432卷;第363页倒数第1段至第364页第1段，表14.1，摘要 *

Also Published As

Publication number Publication date
EP3174991A1 (en) 2017-06-07
EP3174991A4 (en) 2018-02-14
CA2955204A1 (en) 2016-02-04
JP2017524708A (ja) 2017-08-31
AU2015296304A1 (en) 2017-02-02
WO2016019140A1 (en) 2016-02-04
JP6810023B2 (ja) 2021-01-06
AU2015296304B2 (en) 2019-08-29
EP3174991B1 (en) 2024-03-20
US20170192023A1 (en) 2017-07-06
US11181535B2 (en) 2021-11-23
CN107075539A (zh) 2017-08-18
ES2978375T3 (es) 2024-09-11

Similar Documents

Publication Publication Date Title
US11181535B2 (en) Isotopically-labeled cholesteryl ester internal standard composition and kit
Aldana et al. Exploring the lipidome: current lipid extraction techniques for mass spectrometry analysis
Jurowski et al. Analytical techniques in lipidomics: state of the art
Wiesner et al. Lipid profiling of FPLC-separated lipoprotein fractions by electrospray ionization tandem mass spectrometry
EP2607902B1 (en) Markers of non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) and methods of use thereof
Witting et al. The Caenorhabditis elegans lipidome: A primer for lipid analysis in Caenorhabditis elegans
Chen et al. Atherosclerotic dyslipidemia revealed by plasma lipidomics on ApoE−/− mice fed a high-fat diet
Liu et al. Lipidomics in milk: Recent advances and developments
CN112739441A (zh) 用于脂质定量的系统和方法
CN105120852A (zh) 评估生物样品质量的工具和方法
Flis et al. Phosphatidylcholine supply to peroxisomes of the yeast Saccharomyces cerevisiae
EP2286245A2 (en) Means and methods for assessing increased peroxisomal proliferation
JP2017524708A5 (enExample)
Nakanishi et al. Qualitative and quantitative analyses of phospholipids by LC–MS for lipidomics
McDonald et al. Approaches to lipid analysis
Astarita et al. Lipidomic analysis of biological samples by liquid chromatography coupled to mass spectrometry
Holcapek et al. Mass spectrometry for lipidomics: methods and applications
Berry et al. Analysis of polyunsaturated aminophospholipid molecular species using isotope-tagged derivatives and tandem mass spectrometry/mass spectrometry/mass spectrometry
Hewelt-Belka et al. Analytical strategies and applications in lipidomics
Markello et al. High-performance liquid chromatography of lipids for the identification of human metabolic disease
Vecchini et al. Analysis of phospholipid molecular species
Mateckaja et al. Detergent-resistant membranes in HeLa cells. A comparative study with an electrochemical and lipidomic perspective
Kumar et al. Background of Membrane Lipids
Li Development of Metabolomics and Lipidomics Workflows for Phosphorylated Metabolites Using Ultra-High-Performance Liquid Chromatography with Tandem Mass Spectrometry
Mhatre et al. Evaluation of erythrocyte membrane lipids and proteins in renal disorders

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant