CN107050128A - A kind of antitumor giant knotweed compound preparation and preparation method thereof - Google Patents
A kind of antitumor giant knotweed compound preparation and preparation method thereof Download PDFInfo
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- CN107050128A CN107050128A CN201710321147.4A CN201710321147A CN107050128A CN 107050128 A CN107050128 A CN 107050128A CN 201710321147 A CN201710321147 A CN 201710321147A CN 107050128 A CN107050128 A CN 107050128A
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Abstract
The invention discloses a kind of antitumor giant knotweed compound preparation and preparation method thereof, said preparation is prepared from by the raw material of following parts by weight：Orange 10 15 parts of the extract of 30 50 parts of Gentrin Knotweed P.E, 10 20 parts of selenium malt flour, 5 10 parts of Silkworm pupa protein, 5 10 parts of the fruit of Cherokee rose, 5 10 parts of nostoc yeast powder, 5 10 parts of hickory nut bark, 10 15 parts of Olive leaf P.E, 5 10 parts of airpotato yam, 5 10 parts of spirulina freeze-dried powder, 10 15 parts of beet root extract, blood, 6 10 parts of curcuma zedoary, 15 20 parts of cepehalotaxus fortunei extract, 10 20 parts of wilsonii, 10 20 parts of the Radix Astragali, 7 13 parts of hop, 5 10 parts of valerian.The present invention has detoxicating and resolving a mass, promoting blood circulation, supplementing qi and nourishing yin, and killing cancer cell, suppression growth of cancer cells, symptom management develop, improve immunologic function, the effect of pre- preventing tumor.
Description
Technical field
The present invention relates to field of traditional Chinese, and in particular to a kind of antitumor giant knotweed compound preparation and preparation method thereof.
Background technology
Tumour is a kind of common disease, frequently-occurring disease, is body under the effect of various tumorigenesis factors, and the cell of local organization is in base
Because having lost the normal regulation grown to it in level, cause neoformation formed by paraplasm.Antitumor Chinese medicine is one
Effective approach, therapeutic action of the Chinese Traditional Medicine to tumour is increasingly becoming the focus of antitumor research, and achieve it is larger enter
Step.
Polygonum cuspidatum Sieb. et Zucc is the dry root and rhizome of polygonaceae plant giant knotweed, mainly originates in the ground such as Jiangxi, Sichuan, Jiangsu, Shandong,
Its cold nature, mildly bitter flavor, Return liver, courage, lung channel have effects that clearing heat and detoxicating, preventing phlegm from forming and stopping coughing, removing dampness through diuresis and removing jaundice, blood stasis removing analgesic.Tiger
Mainly contain the Multiple components such as anthraquinone, diphenylethylene compounds in cane, wherein anthraquinone derivatives, based on sequestered, have
The compositions such as rheum emodin, Physcion, Chrysophanol, with antibacterial, liver protection, rush down under, the function such as anticancer；And diphenylethylene chemical combination
Thing mainly includes resveratrol and polygonin (polydatin), with antitumor, reducing blood lipid, suppresses platelet aggregation, tune
Save the multiple pharmacological effect such as lipoprotein metabolism, liver protection, anti-oxidant, antibacterial.
The content of the invention
To solve the above problems, the invention provides a kind of antitumor giant knotweed compound preparation and preparation method thereof, with solution
Malicious dissipating bind, promoting blood circulation, supplementing qi and nourishing yin, killing cancer cell, suppress growth of cancer cells, symptom management development, improve immunologic function,
The effect of pre- preventing tumor.The medicine uses natural component, takes safety mostly, and non-evident effect has no through clinical observation for many years
Patient has adverse reaction, is adapted to patient's long-term taking.
To achieve the above object, the technical scheme taken of the present invention is：
A kind of antitumor giant knotweed compound preparation, is prepared from by the raw material of following parts by weight：
30-50 parts of Gentrin Knotweed P.E, 10-20 parts of selenium malt flour, 5-10 parts of Silkworm pupa protein, 5-10 parts of the fruit of Cherokee rose, Ge Xian
5-10 parts of yeast powder of rice, 5-10 parts of hickory nut bark, 10-15 parts of Olive leaf P.E, 5-10 parts of airpotato yam, spirulina freeze-dried powder
5-10 parts, 10-15 parts of beet root extract, orange 10-15 parts of the extract of blood, 6-10 parts of curcuma zedoary, 15-20 parts of cepehalotaxus fortunei extract,
10-20 parts of wilsonii, 10-20 parts of the Radix Astragali, 7-13 parts of hop, 5-10 parts of valerian.
Wherein, the Gentrin Knotweed P.E is as obtained by prepared by following steps：
S1, by after clean giant knotweed rhizome and root stripping and slicing, add 3-8 times of water, add by mass percentage for 2-3% ratio
Enter cellulase, added for 1-2% at beta-glucosidase, 30-50 DEG C digest 1-2h by mass percentage, filtered, must digest
Liquid；
S2, in the enzymolysis liquid of gained add 6-10 times of volume 90% ethanol solution, handle 1-3 minutes, separation of solid and liquid
Afterwards, ethanol solution, concentration are reclaimed so that extract is in paste, then extract is extracted 3 times with water-saturated n-butanol, is reclaimed
After solvent, freeze-drying obtains Gentrin Knotweed P.E.
Wherein, the Silkworm pupa protein is as obtained by prepared by following steps：
S1, the silkworm chrysalis feeding steam steaming after draining will be cleaned, steam steaming is divided into three phases, and the first stage keeps pressure
Power is 6-9Mpa, 65-95 degrees Celsius of temperature, time 5-6 hour, and second stage keeps pressure to be that 9-11Mpa, temperature 95-115 take the photograph
Family name's degree, time 10-12 hour, the phase III keep pressure to be 3-5Mpa, 70-90 degrees Celsius of temperature, time 7-8 hour；
S2, the silkworm chrysalis for completing steam steaming obtain degreasing pupa albumen through supercritical carbon dioxide extracting degreasing；
S3, call in ethanol and carry out gradient agitator treating, a upper strata high position extracts ethanol washing lotion and pupa cortex out after washing, reclaims second
Alcohol, lower floor's centrifugation obtains silkworm chrysalis protein concentrate；
S4, in silkworm chrysalis protein concentrate quality percentage for 0.5-2% ratio neutral proteinase is added, enzyme at 30-50 DEG C
0.5-2h is solved, filtering obtains enzymolysis liquid；
S5, by naturally to thaw again after the enzymolysis liquid quick freeze of gained, precipitate the age of starch in enzymolysis liquid；In collection
Clear liquid, remainder centrifugation is collected centrifugal liquid and is incorporated in supernatant；
S6, the Compound-acid solution for adding 2-3 times of volume, connection ozone generator ozone handle deodorization, then steam stripping at reduced pressure
Deacidification and bad smell are gone in concentration, and freeze-drying obtains Silkworm pupa protein.
Wherein, the nostoc yeast powder is as obtained by prepared by following steps：
The nostoc cleaned after draining is taken to be placed in steam-explosion jar, it is 0.7- to be first passed through nitrogen to steam explosion pressure inside the tank
1.5MPa, explosion treatment 7-23min；Then it is at 1.5-2.1MPa, steam blasting to be passed through steam to steam explosion pressure inside the tank rapidly
Manage after 0.8-2.8min, according to inoculum concentration 1.5-1.7% ratio microbe inoculation leavening, 25-35 DEG C of keeping temperature, fermentation
After 48-54 hours, high-temperature sterilization, spray drying obtains nostoc yeast powder.
Wherein, the microbe leaven is by Bacillus natto, ferment bacterium, Corynebacterium glutamicum in mass ratio 3:2:1 mixing institute
.
Wherein, the Olive leaf P.E is as obtained by prepared by following steps：
Take olive leaf to be freeze-dried after cleaning, smash, room temperature is concentrated into relative density 1.10-1.20 after defrosting, spraying is dry
After dry concentrate, CO is carried out2Supercritical extract, fluid extraction pressure is 30-50MPa, 30-40 DEG C of extraction temperature, CO2Fluid stream
Measure as 750-850L/h, extraction time is 3-5h, obtains Olive leaf P.E.
Wherein, the spirulina freeze-dried powder is as obtained by prepared by following steps：
It is placed in after clean spirulina is handled through ultrasonication in autoclave, adds the water of 2-3 times of powder, be passed through nitrogen
Pressure is 0.7-1.5MPa in autoclave, handles 8-25min；Then it is 1.5- to be passed through pressure in steam to autoclave rapidly
2.5Mpa, freeze-drying, obtains spirulina freeze-dried powder.
Present invention also offers a kind of above-mentioned preparation method of antitumor giant knotweed compound preparation, comprise the following steps：
S1, by above-mentioned formula weigh each component；
S2, by the fruit of Cherokee rose weighed, hickory nut bark, airpotato yam, curcuma zedoary, wilsonii, the Radix Astragali, hop, valerian dodge
Formula extractor processing, gained extract solution first heats with high heat, after boiling with slow fire heat 1-1.5h, filtering and concentrating to 1.5g/ml,
90-100% ethanol is slowly added to, until concentration of alcohol is 55-65%, is refrigerated 8-9 hours, 2000-4000r/min centrifugations 15-
Supernatant is removed after 25min, precipitation is dissolved to original volume with deionized water, with ethanol precipitation 2 times, concentration of alcohol is respectively 65-
75%th, 75-85%；Precipitation is dissolved to original volume with deionized water, adds 1/6-1/5 volumes 10-12% solution of trichloroacetic acid,
Go to precipitate to obtain supernatant after standing 4-6h, 2000-4000r/min centrifugations 15-25min after fully mixing, gained supernatant is taken out
Filter, obtains extract；
S3, carried extract obtained with Gentrin Knotweed P.E, selenium malt flour, Silkworm pupa protein, nostoc yeast powder, olive leaf
Take after the orange extract of thing, spirulina freeze-dried powder, beet root extract, blood and cepehalotaxus fortunei extract be mixed evenly, freezing is dry
Dry, sterilizing is packed by specification, produces preparation.
The invention has the advantages that：
By using homemade Gentrin Knotweed P.E as main ingredient, cooperateing with selenium malt flour, Silkworm pupa protein, nostoc yeast powder, olive
The effect of olive leaf extract etc., with detoxicating and resolving a mass, promoting blood circulation, supplementing qi and nourishing yin, killing cancer cell, suppress growth of cancer cells,
Symptom management development, raising immunologic function, the effect of pre- preventing tumor.The medicine uses natural component, safety is taken, without substantially mostly
Side effect, has no that patient has adverse reaction through clinical observation for many years, is adapted to patient's long-term taking.
Embodiment
In order that objects and advantages of the present invention are more clearly understood, the present invention is carried out with reference to embodiments further
Describe in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair
It is bright.
Gentrin Knotweed P.E used in following examples is as obtained by prepared by following steps：
S1, by after clean giant knotweed rhizome and root stripping and slicing, add 3-8 times of water, add by mass percentage for 2-3% ratio
Enter cellulase, added for 1-2% at beta-glucosidase, 30-50 DEG C digest 1-2h by mass percentage, filtered, must digest
Liquid；
S2, in the enzymolysis liquid of gained add 6-10 times of volume 90% ethanol solution, handle 1-3 minutes, separation of solid and liquid
Afterwards, ethanol solution, concentration are reclaimed so that extract is in paste, then extract is extracted 3 times with water-saturated n-butanol, is reclaimed
After solvent, freeze-drying obtains Gentrin Knotweed P.E.
Used Silkworm pupa protein is as obtained by prepared by following steps：
S1, the silkworm chrysalis feeding steam steaming after draining will be cleaned, steam steaming is divided into three phases, and the first stage keeps pressure
Power is 6-9Mpa, 65-95 degrees Celsius of temperature, time 5-6 hour, and second stage keeps pressure to be that 9-11Mpa, temperature 95-115 take the photograph
Family name's degree, time 10-12 hour, the phase III keep pressure to be 3-5Mpa, 70-90 degrees Celsius of temperature, time 7-8 hour；
S2, the silkworm chrysalis for completing steam steaming obtain degreasing pupa albumen through supercritical carbon dioxide extracting degreasing；
S3, call in ethanol and carry out gradient agitator treating, a upper strata high position extracts ethanol washing lotion and pupa cortex out after washing, reclaims second
Alcohol, lower floor's centrifugation obtains silkworm chrysalis protein concentrate；
S4, in silkworm chrysalis protein concentrate quality percentage for 0.5-2% ratio neutral proteinase is added, enzyme at 30-50 DEG C
0.5-2h is solved, filtering obtains enzymolysis liquid；
S5, by naturally to thaw again after the enzymolysis liquid quick freeze of gained, precipitate the age of starch in enzymolysis liquid；In collection
Clear liquid, remainder centrifugation is collected centrifugal liquid and is incorporated in supernatant；
S6, the Compound-acid solution for adding 2-3 times of volume, connection ozone generator ozone handle deodorization, then steam stripping at reduced pressure
Deacidification and bad smell are gone in concentration, and freeze-drying obtains Silkworm pupa protein.
Used nostoc yeast powder is as obtained by prepared by following steps：
The nostoc cleaned after draining is taken to be placed in steam-explosion jar, it is 0.7- to be first passed through nitrogen to steam explosion pressure inside the tank
1.5MPa, explosion treatment 7-23min；Then it is at 1.5-2.1MPa, steam blasting to be passed through steam to steam explosion pressure inside the tank rapidly
Manage after 0.8-2.8min, according to inoculum concentration 1.5-1.7% ratio microbe inoculation leavening, 25-35 DEG C of keeping temperature, fermentation
After 48-54 hours, high-temperature sterilization, spray drying obtains nostoc yeast powder.
Used microbe leaven is by Bacillus natto, ferment bacterium, Corynebacterium glutamicum in mass ratio 3:2:1 mixing institute
.
Used Olive leaf P.E is as obtained by prepared by following steps：
Take olive leaf to be freeze-dried after cleaning, smash, room temperature is concentrated into relative density 1.10-1.20 after defrosting, spraying is dry
After dry concentrate, CO is carried out2Supercritical extract, fluid extraction pressure is 30-50MPa, 30-40 DEG C of extraction temperature, CO2Fluid stream
Measure as 750-850L/h, extraction time is 3-5h, obtains Olive leaf P.E.
Used spirulina freeze-dried powder is as obtained by prepared by following steps：
It is placed in after clean spirulina is handled through ultrasonication in autoclave, adds the water of 2-3 times of powder, be passed through nitrogen
Pressure is 0.7-1.5MPa in autoclave, handles 8-25min；Then it is 1.5- to be passed through pressure in steam to autoclave rapidly
2.5Mpa, freeze-drying, obtains spirulina freeze-dried powder.
Embodiment 1
A kind of preparation method of antitumor giant knotweed compound preparation, comprises the following steps：
S1, weigh by weight：30 parts of Gentrin Knotweed P.E, 10 parts of selenium malt flour, 5 parts of Silkworm pupa protein, 5 parts of the fruit of Cherokee rose,
5 parts of nostoc yeast powder, 5 parts of hickory nut bark, 10 parts of Olive leaf P.E, 5 parts of airpotato yam, 5 parts of spirulina freeze-dried powder, beet
Orange 10 parts of the extract of 10 parts of root extract, blood, 6 parts of curcuma zedoary, 15 parts of cepehalotaxus fortunei extract, 10 parts of wilsonii, 10 parts of the Radix Astragali, golden hop
Careless 7 parts, 5 parts of valerian；
S2, by the fruit of Cherokee rose weighed, hickory nut bark, airpotato yam, curcuma zedoary, wilsonii, the Radix Astragali, hop, valerian dodge
Formula extractor processing, gained extract solution first heats with high heat, after boiling with slow fire heat 1-1.5h, filtering and concentrating to 1.5g/ml,
90-100% ethanol is slowly added to, until concentration of alcohol is 55-65%, is refrigerated 8-9 hours, 2000-4000r/min centrifugations 15-
Supernatant is removed after 25min, precipitation is dissolved to original volume with deionized water, with ethanol precipitation 2 times, concentration of alcohol is respectively 65-
75%th, 75-85%；Precipitation is dissolved to original volume with deionized water, adds 1/6-1/5 volumes 10-12% solution of trichloroacetic acid,
Go to precipitate to obtain supernatant after standing 4-6h, 2000-4000r/min centrifugations 15-25min after fully mixing, gained supernatant is taken out
Filter, obtains extract；
S3, carried extract obtained with Gentrin Knotweed P.E, selenium malt flour, Silkworm pupa protein, nostoc yeast powder, olive leaf
Take after the orange extract of thing, spirulina freeze-dried powder, beet root extract, blood and cepehalotaxus fortunei extract be mixed evenly, freezing is dry
Dry, sterilizing is packed by specification, produces preparation.
Embodiment 2
A kind of preparation method of antitumor giant knotweed compound preparation, comprises the following steps：
S1, weigh by weight：50 parts of Gentrin Knotweed P.E, 20 parts of selenium malt flour, 10 parts of Silkworm pupa protein, the fruit of Cherokee rose 10
Part, 10 parts of nostoc yeast powder, 10 parts of hickory nut bark, 15 parts of Olive leaf P.E, 10 parts of airpotato yam, spirulina freeze-dried powder 10
Part, orange 15 parts of the extract of 15 parts of beet root extract, blood, 10 parts of curcuma zedoary, 20 parts of cepehalotaxus fortunei extract, 20 parts of wilsonii, the Radix Astragali
20 parts, 13 parts of hop, 10 parts of valerian；
S2, by the fruit of Cherokee rose weighed, hickory nut bark, airpotato yam, curcuma zedoary, wilsonii, the Radix Astragali, hop, valerian dodge
Formula extractor processing, gained extract solution first heats with high heat, after boiling with slow fire heat 1-1.5h, filtering and concentrating to 1.5g/ml,
90-100% ethanol is slowly added to, until concentration of alcohol is 55-65%, is refrigerated 8-9 hours, 2000-4000r/min centrifugations 15-
Supernatant is removed after 25min, precipitation is dissolved to original volume with deionized water, with ethanol precipitation 2 times, concentration of alcohol is respectively 65-
75%th, 75-85%；Precipitation is dissolved to original volume with deionized water, adds 1/6-1/5 volumes 10-12% solution of trichloroacetic acid,
Go to precipitate to obtain supernatant after standing 4-6h, 2000-4000r/min centrifugations 15-25min after fully mixing, gained supernatant is taken out
Filter, obtains extract；
S3, carried extract obtained with Gentrin Knotweed P.E, selenium malt flour, Silkworm pupa protein, nostoc yeast powder, olive leaf
Take after the orange extract of thing, spirulina freeze-dried powder, beet root extract, blood and cepehalotaxus fortunei extract be mixed evenly, freezing is dry
Dry, sterilizing is packed by specification, produces preparation.
Embodiment 3
A kind of preparation method of antitumor giant knotweed compound preparation, comprises the following steps：
S1, weigh by weight：40 parts of Gentrin Knotweed P.E, 15 parts of selenium malt flour, 7.5 parts of Silkworm pupa protein, the fruit of Cherokee rose 7.5
Part, 7.5 parts of nostoc yeast powder, 7.5 parts of hickory nut bark, 12.5 parts of Olive leaf P.E, 7.5 parts of airpotato yam, spirulina freeze
Orange 12.5 parts of the extract of 7.5 parts of dry powder, 7.5 parts of beet root extract, blood, 8 parts of curcuma zedoary, 17.5 parts of cepehalotaxus fortunei extract, thorn five
Plus 15 parts, 15 parts of the Radix Astragali, 10 parts of hop, 7.5 parts of valerian；
S2, by the fruit of Cherokee rose weighed, hickory nut bark, airpotato yam, curcuma zedoary, wilsonii, the Radix Astragali, hop, valerian dodge
Formula extractor processing, gained extract solution first heats with high heat, after boiling with slow fire heat 1-1.5h, filtering and concentrating to 1.5g/ml,
90-100% ethanol is slowly added to, until concentration of alcohol is 55-65%, is refrigerated 8-9 hours, 2000-4000r/min centrifugations 15-
Supernatant is removed after 25min, precipitation is dissolved to original volume with deionized water, with ethanol precipitation 2 times, concentration of alcohol is respectively 65-
75%th, 75-85%；Precipitation is dissolved to original volume with deionized water, adds 1/6-1/5 volumes 10-12% solution of trichloroacetic acid,
Go to precipitate to obtain supernatant after standing 4-6h, 2000-4000r/min centrifugations 15-25min after fully mixing, gained supernatant is taken out
Filter, obtains extract；
S3, carried extract obtained with Gentrin Knotweed P.E, selenium malt flour, Silkworm pupa protein, nostoc yeast powder, olive leaf
Take after the orange extract of thing, spirulina freeze-dried powder, beet root extract, blood and cepehalotaxus fortunei extract be mixed evenly, freezing is dry
Dry, sterilizing is packed by specification, produces preparation
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (8)
1. a kind of antitumor giant knotweed compound preparation, it is characterised in that be prepared from by the raw material of following parts by weight：
30-50 parts of Gentrin Knotweed P.E, 10-20 parts of selenium malt flour, 5-10 parts of Silkworm pupa protein, 5-10 parts of the fruit of Cherokee rose, nostoc hair
5-10 parts of ferment powder, 5-10 parts of hickory nut bark, 10-15 parts of Olive leaf P.E, 5-10 parts of airpotato yam, spirulina freeze-dried powder 5-10
Part, orange 10-15 parts of the extract of 10-15 parts of beet root extract, blood, 6-10 parts of curcuma zedoary, 15-20 parts of cepehalotaxus fortunei extract, thorn five
Plus 10-20 parts, 10-20 parts of the Radix Astragali, 7-13 parts of hop, 5-10 parts of valerian.
2. a kind of antitumor giant knotweed compound preparation as claimed in claim 1, it is characterised in that the Gentrin Knotweed P.E by with
Lower step prepares gained：
S1, by after clean giant knotweed rhizome and root stripping and slicing, add 3-8 times of water, be by mass percentage 2-3% ratio add it is fine
The plain enzyme of dimension, adds at beta-glucosidase, 30-50 DEG C for 1-2% digest 1-2h by mass percentage, filter, obtain enzymolysis liquid；
S2, in the enzymolysis liquid of gained add 6-10 times of volume 90% ethanol solution, handle 1-3 minute, after separation of solid and liquid, time
Receive ethanol solution, concentration so that extract is in paste, then extract is extracted 3 times with water-saturated n-butanol, recycling design
Afterwards, it is freeze-dried, obtains Gentrin Knotweed P.E.
3. a kind of antitumor giant knotweed compound preparation as claimed in claim 1, it is characterised in that the Silkworm pupa protein by with
Lower step prepares gained：
S1, the silkworm chrysalis feeding steam steaming after draining will be cleaned, steam steaming is divided into three phases, the first stage keeps the pressure to be
6-9Mpa, 65-95 degrees Celsius of temperature, time 5-6 hour, second stage keep pressure to be that 9-11Mpa, temperature 95-115 are Celsius
Degree, time 10-12 hour, the phase III keep pressure to be 3-5Mpa, 70-90 degrees Celsius of temperature, time 7-8 hour；
S2, the silkworm chrysalis for completing steam steaming obtain degreasing pupa albumen through supercritical carbon dioxide extracting degreasing；
S3, call in ethanol and carry out gradient agitator treating, a upper strata high position extracts ethanol washing lotion and pupa cortex out after washing, reclaims ethanol,
Lower floor's centrifugation obtains silkworm chrysalis protein concentrate；
S4, in silkworm chrysalis protein concentrate quality percentage for 0.5-2% ratio neutral proteinase is added, digested at 30-50 DEG C
0.5-2h, filtering, obtains enzymolysis liquid；
S5, by naturally to thaw again after the enzymolysis liquid quick freeze of gained, precipitate the age of starch in enzymolysis liquid；Collect supernatant
Liquid, remainder centrifugation is collected centrifugal liquid and is incorporated in supernatant；
S6, the Compound-acid solution for adding 2-3 times of volume, connection ozone generator ozone processing deodorization, then steam stripping at reduced pressure concentration
Deacidification and bad smell are gone, freeze-drying obtains Silkworm pupa protein.
4. a kind of antitumor giant knotweed compound preparation as claimed in claim 1, it is characterised in that the nostoc yeast powder passes through
Following steps prepare gained：
The nostoc cleaned after draining is taken to be placed in steam-explosion jar, it is 0.7-1.5MPa to be first passed through nitrogen to steam explosion pressure inside the tank, quick-fried
Tear manages 7-23min；Then it is 1.5-2.1MPa, Steam explosion treatment 0.8- to be passed through steam to steam explosion pressure inside the tank rapidly
After 2.8min, according to inoculum concentration 1.5-1.7% ratio microbe inoculation leavening, 25-35 DEG C of keeping temperature, ferment 48-54
After hour, high-temperature sterilization, spray drying obtains nostoc yeast powder.
5. a kind of antitumor giant knotweed compound preparation as claimed in claim 4, it is characterised in that the microbe leaven is by receiving
Beans bacterium, ferment bacterium, Corynebacterium glutamicum in mass ratio 3:2:1 mixing gained.
6. a kind of antitumor giant knotweed compound preparation as claimed in claim 1, it is characterised in that the Olive leaf P.E passes through
Following steps prepare gained：
Take olive leaf to be freeze-dried after cleaning, smash, room temperature is concentrated into relative density 1.10-1.20 after defrosting, be spray-dried dense
After contracting liquid, CO is carried out2Supercritical extract, fluid extraction pressure is 30-50MPa, 30-40 DEG C of extraction temperature, CO2Fluid flow is
750-850L/h, extraction time is 3-5h, obtains Olive leaf P.E.
7. a kind of antitumor giant knotweed compound preparation as claimed in claim 1, it is characterised in that the spirulina freeze-dried powder passes through
Following steps prepare gained：
It is placed in after clean spirulina is handled through ultrasonication in autoclave, adds the water of 2-3 times of powder, be passed through nitrogen paramount
It is 0.7-1.5MPa to press pressure in kettle, handles 8-25min；Then it is 1.5- to be passed through pressure in steam to autoclave rapidly
2.5Mpa, freeze-drying, obtains spirulina freeze-dried powder.
8. a kind of preparation method of antitumor giant knotweed compound preparation, it is characterised in that comprise the following steps：
S1, the formula as described in claim any one of 1-7 weigh each component；
S2, the fruit of Cherokee rose weighed, hickory nut bark, airpotato yam, curcuma zedoary, wilsonii, the Radix Astragali, hop, valerian carried with flash
Device is taken to handle, gained extract solution is first heated with high heat, with slow fire heating 1-1.5h after boiling, filtering and concentrating to 1.5g/ml, slowly
90-100% ethanol is added, until concentration of alcohol is 55-65%, is refrigerated 8-9 hours, 2000-4000r/min centrifugations 15-25min
Remove supernatant afterwards, precipitation is dissolved to original volume with deionized water, with ethanol precipitation 2 times, concentration of alcohol be respectively 65-75%,
75-85%；Precipitation is dissolved to original volume with deionized water, adds 1/6-1/5 volumes 10-12% solution of trichloroacetic acid, fully
Go to precipitate to obtain supernatant after standing 4-6h, 2000-4000r/min centrifugations 15-25min after mixing, gained supernatant suction filtration is obtained
Extract；
S3, by it is extract obtained with Gentrin Knotweed P.E, selenium malt flour, Silkworm pupa protein, nostoc yeast powder, olive leaf extract
After the orange extract of thing, spirulina freeze-dried powder, beet root extract, blood and cepehalotaxus fortunei extract are mixed evenly, freeze-drying,
Sterilizing, is packed by specification, produces preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710321147.4A CN107050128A (en) | 2017-05-09 | 2017-05-09 | A kind of antitumor giant knotweed compound preparation and preparation method thereof |
Applications Claiming Priority (1)
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| CN201710321147.4A CN107050128A (en) | 2017-05-09 | 2017-05-09 | A kind of antitumor giant knotweed compound preparation and preparation method thereof |
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| CN107789580A (en) * | 2017-11-20 | 2018-03-13 | 郑州大学 | A kind of medicine for treating tumour and preparation method thereof |
| CN109793877A (en) * | 2019-04-03 | 2019-05-24 | 赤峰市肿瘤医院(赤峰学院第二附属医院) | A kind of Chinese medicine composition for treating tumour |
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| CN105316774A (en) * | 2015-10-27 | 2016-02-10 | 湖州大成纺织有限公司 | White steam filature production technology |
| CN106560202A (en) * | 2016-11-21 | 2017-04-12 | 李军凯 | Chinese herbal preparation for treating tumor |
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| CN105316774A (en) * | 2015-10-27 | 2016-02-10 | 湖州大成纺织有限公司 | White steam filature production technology |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107789580A (en) * | 2017-11-20 | 2018-03-13 | 郑州大学 | A kind of medicine for treating tumour and preparation method thereof |
| CN109793877A (en) * | 2019-04-03 | 2019-05-24 | 赤峰市肿瘤医院(赤峰学院第二附属医院) | A kind of Chinese medicine composition for treating tumour |
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Application publication date: 20170818 |