CN106560202A - Chinese herbal preparation for treating tumor - Google Patents
Chinese herbal preparation for treating tumor Download PDFInfo
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- CN106560202A CN106560202A CN201611058906.4A CN201611058906A CN106560202A CN 106560202 A CN106560202 A CN 106560202A CN 201611058906 A CN201611058906 A CN 201611058906A CN 106560202 A CN106560202 A CN 106560202A
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Abstract
The invention discloses a Chinese herbal preparation for treating tumor. The Chinese herbal preparation for treating tumor is prepared from, by weight, 15-25 parts of euchresta japonica, 10-15 parts of milk thistle extracts, 10-15 parts of leaves of red dates, 8-14 parts of mussaenda parviflora Matsum, 12-18 parts of dendrobium extracts, 15-20 parts of flosmume, 12-18 parts of freeze-dried power of nostoc sphaeroides, 5-15 parts of tree barks of hickory, 10-15 parts of extracts of olive leaves, 10-15 parts of grape fermentation powder, 7-13 parts of humulus lupulus, 5-10 parts of valeriana officinalis L., 10-20 parts of concha ostreae, 13-27 parts of scutellaria barbata D. Don, 5-10 parts of freeze-dried power of Indian mockstrawberrg herb, 10-20 parts of eleutherococcus senticosus, and 10-20 parts of radix astragali. The Chinese herbal preparation for treating tumor has the functions of eliminating toxic materials and dispersing stasis, invigorating blood circulation and removing blood stasis, tonifying qi and nourishing yin, killing cancer cells, inhibiting the growth of the cancer cells, controlling progression of diseases and improving immunologic functions. Natural ingredients are mainly adopted for preparing the Chinese herbal preparation for treating tumor, and the Chinese herbal preparation for treating tumor is safe to take, has no obvious side effects, and is suitable for a patient to take for a long time.
Description
Technical field
The present invention relates to field of traditional Chinese, and in particular to a kind of Chinese medicine preparation for treating tumor.
Background technology
Tumor is body under the effect of various carcinogenic factors, and some cell of local organization loses right on gene level
The normal regulation of its growth, the neoplasm for causing its clonal abnormality hypertrophy and being formed, tumor are generally divided into benign tumor and evil
Property tumor.Malignant tumor can destroy the 26S Proteasome Structure and Function of tissue, organ, be the commonly encountered diseases for seriously threatening human health so far
One of with frequently-occurring disease, effective antitumour medicine and method are found, it is that the important of world medical circle is ground thoroughly to capture malignant tumor
Study carefully problem.
The traditional Chinese medical science thinks that tumor is systemic disease, weakened body resistance blood stasis, residual poison clearly be its pathogenic characteristic, neoplasm metastasis it is basic
Reason is healthy energy virtual loss, is accumulate in evil poison.No matter perform the operation or chemotherapeutic treatment, uncharm purpose though having reached,
Patient's internal organs, negative and positive, the imbalance of QI and blood function are not fundamentally released, determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs and whole will be particular about according to Chinese traditional treatment tumor
Body is treated, and radiotherapy, chemotherapy are included dialectical medication category, is formed doctor trained in Western medicine and is attacked heresy, the general layout of traditional Chinese medical science righting, using the combination of Chinese and Western medicine
Method carry out oncotherapy and tend to obtain preferable effect, Chinese medicine to the auxiliary therapeutic action of tumor patient also increasingly
To accreditation.
The medicine for being presently used for treating tumor is a lot, but all there is the defect of large side effects in various degree, serious shadow
Ring the treatment and rehabilitation of patient.
The content of the invention
Object of the present invention is to provide a kind of Chinese medicine preparation for treating tumor, with detoxicating and resolving stagnation of pathogens, blood circulation promoting and blood stasis dispelling, benefit
Gas yin nourishing, kills cancerous cell, anticancer growth, symptom management development, the effect for improving immunologic function.The medicine is adopted mostly
Natural component, safety is taken, non-evident effect is adapted to patient's life-time service.
For achieving the above object, the technical scheme taken of the present invention is：
A kind of Chinese medicine preparation for treating tumor, is prepared from by the component of following weight portion：
Radix Sophorae Tonkinensiss 15-25 parts, Milk Thistle extract 10-15 parts, Fructus Jujubae leaf 10-15 parts, Mussaenda 8-14 parts, Herba Dendrobii are extracted
Thing 12-18 parts, FLOS MUME 15-20 part, Nostoc lyophilized powder 12-18 parts, Semen Caryae Cathayensis bark 5-15 parts, Olive leaf P.E 10-
15 parts, grape fermentation powder 10-15 parts, hop 7-13 parts, Rhizoma et radix valerianae 5-10 parts, Concha Ostreae 10-20 parts, Herba Scutellariae Barbatae 13-27 parts, Herba Duchesneae Indicae
Lyophilized powder 5-10 parts, Radix Et Caulis Acanthopanacis Senticosi 10-20 parts, Radix Astragali 10-20 parts.
Preferably, it is prepared from by the component of following weight portion：
15 parts of Radix Sophorae Tonkinensiss, 10 parts of Milk Thistle extract, 10 parts of Fructus Jujubae leaf, 8 parts of Mussaenda, 12 parts of Herba Dendrobii extract, green calyx
15 parts of prunus mume (sieb.) sieb.et zucc., 12 parts of Nostoc lyophilized powder, 5 parts of Semen Caryae Cathayensis bark, 10 parts of Olive leaf P.E, 10 parts of grape fermentation powder, hop 7
Part, 5 parts of Rhizoma et radix valerianae, 10 parts of Concha Ostreae, 13 parts of Herba Scutellariae Barbatae, 5 parts of Herba Duchesneae Indicae lyophilized powder, 10 parts of Radix Et Caulis Acanthopanacis Senticosi, 10 parts of the Radix Astragali.
Preferably, it is prepared from by the component of following weight portion：
25 parts of Radix Sophorae Tonkinensiss, 15 parts of Milk Thistle extract, 15 parts of Fructus Jujubae leaf, 14 parts of Mussaenda, 18 parts of Herba Dendrobii extract, green calyx
20 parts of prunus mume (sieb.) sieb.et zucc., 18 parts of Nostoc lyophilized powder, 15 parts of Semen Caryae Cathayensis bark, 15 parts of Olive leaf P.E, 15 parts of grape fermentation powder, hop
13 parts, 10 parts of Rhizoma et radix valerianae, 20 parts of Concha Ostreae, 27 parts of Herba Scutellariae Barbatae, 10 parts of Herba Duchesneae Indicae lyophilized powder, 20 parts of Radix Et Caulis Acanthopanacis Senticosi, 20 parts of the Radix Astragali.
Preferably, it is prepared from by the component of following weight portion：
20 parts of Radix Sophorae Tonkinensiss, 12.5 parts of Milk Thistle extract, 12.5 parts of Fructus Jujubae leaf, 11 parts of Mussaenda, 15 parts of Herba Dendrobii extract,
17.5 parts of FLOS MUME, 15 parts of Nostoc lyophilized powder, 10 parts of Semen Caryae Cathayensis bark, 12.5 parts of Olive leaf P.E, grape fermentation powder
12.5 parts, it is 10 parts of hop, 7.5 parts of Rhizoma et radix valerianae, 15 parts of Concha Ostreae, 20 parts of Herba Scutellariae Barbatae, 7.5 parts of Herba Duchesneae Indicae lyophilized powder, 15 parts of Radix Et Caulis Acanthopanacis Senticosi, yellow
15 parts of stilbene.
Preferably, the Milk Thistle extract is as obtained by prepared by following steps：
Dry Milk Thistle is placed in into 90% methanol or ethanol solution that flash extracter adds 6-8 times of volume, 1-3 is processed
Minute, after solid-liquid separation, reclaim methanol or ethanol solution, concentration so that extract is in paste, then with water-saturated n-butanol pair
Extract is extracted 3 times, after recycling design, using titanium rod filter concentrate drying, obtains Milk Thistle extract.
Preferably, the Herba Dendrobii extract is as obtained by prepared by following steps：
S1, the Herba Dendrobii that drains will be cleaned it is placed in steam-explosion jar, it is 0.6- to be first passed through nitrogen to steam explosion pressure inside the tank
1.4MPa, explosion treatment 8-25min；Then it is 1.4-1.8MPa to be passed through rapidly steam to steam explosion pressure inside the tank, at steam explosion
After reason 0.5-2.5min, relative density 1.10-1.20 is concentrated into using titanium rod filter room temperature, is spray-dried concentrated solution, obtains powder
Body；
S2, in powder body add 5-9 times of water, add by cellulase：Neutral protease, flavor protease in mass ratio 1:
The compound enzyme of 2: 3 mixing gained, is combined 3-5% of the enzyme dosage for powder body weight, 1-2h is digested at 25-35 DEG C, filter, must digest
Liquid；
S3, by naturally to thaw again after the fermentation liquid quick freeze of gained, make age of starch precipitation in extract；Collect supernatant
Liquid, remainder centrifugation are collected centrifugal liquid and are incorporated in supernatant；
S4, by supernatant with the flow velocity of 3-7BV/h by macroporous resin column, after dynamic adsorption saturation, deionized water with
The above-mentioned macroporous resin column of flow velocity drip washing of 5-8BV/h is colourless to effluent, then with the organic solvent that volume fraction is 50%-70%
Eluting is carried out with the flow velocity of 8-12BV/h, eluent is collected, concentrating under reduced pressure obtains Herba Dendrobii extract.
Preferably, the Nostoc lyophilized powder is as obtained by prepared by following steps：
Take, lyophilization obtains Nostoc lyophilized powder；Q wherein,
First time explosion treatment keeps pressure for 0.6-0.9Mpa, temperature 65-95 degree Celsius, time 7-23min, second explosion treatment
Holding pressure be 0.9-1.1Mpa, temperature 95-115 degree Celsius, time 0.8-1.2min, third time explosion treatment keep pressure be
3-5Mpa, temperature 70-90 degree Celsius, time 0.8-2.8min.
Preferably, the Olive leaf P.E is as obtained by prepared by following steps：
Will Folium olive clean after be placed in steam-explosion jar, it is 1.5-2.1MPa to be passed through nitrogen to steam explosion pressure inside the tank, and steam is quick-fried
After tear reason 0.8-2.8min, after being cooled to room temperature, alkaline protease is added by the 1-2% of raw material weight, be maintained at 25-35 DEG C
Lower enzymolysis 0.5-2h, filters, obtains enzymolysis solution；By enzymolysis solution directly by neutral alumina column, resin column blade diameter length ratio is 1: 5-1:
10, respectively with 1-4 times of cylinder hydrops, 1-3 times of 95% ethanol solution eluting remove impurity of column volume, with the 0.3- of 5-7 times of column volume
0.6% glacial acetic acid -55% ethanol (1: 1) eluant solution, collects eluent；It is after flinging to the glacial acetic acid and ethanol in eluent, micro-
Ripple is vacuum dried, and obtains Olive leaf P.E.
Preferably, the grape fermentation powder is as obtained by prepared by following steps：
After Fructus Vitis viniferae is cleaned after destemming, broken skin, enter in container, container is sealed with tin foil, after 30h, add Saccharum Sinensis Roxb. 2-3
Part, preservative film being covered after stirring evenly, stirs secondary daily, Pericarpium Vitis viniferae is pressed in juice, nitrogen is passed through after 7d to container inner pressure is
0.7-1.5MPa, processes 8-25min；Then be passed through rapidly steam to container inner pressure be 1.5-2.5Mpa, be cooled to room temperature by
According to the ratio microbe inoculation leaven of inoculum concentration 1.5-1.7%, keeping temperature 25-35 DEG C, after fermentation 48-54h, high temperature goes out
Living, oven drying at low temperature obtains grape fermentation powder.
Preferably, the microbe leaven is by lactobacilluss, Bacillus licheniformis, Corynebacterium glutamicum in mass ratio 1:
3: 2 mixing gained.
Preferably, the Herba Duchesneae Indicae lyophilized powder is by obtained by lyophilization after Fructus Rubi micronizing.
The invention has the advantages that：
With detoxicating and resolving stagnation of pathogens, blood circulation promoting and blood stasis dispelling, supplementing QI and nourishing YIN, cancerous cell, anticancer growth, symptom management are killed
Exhibition, the effect for improving immunologic function.The medicine mostly using natural component, take safety, non-evident effect, be adapted to patient it is long-term
Use.
Specific embodiment
In order that objects and advantages of the present invention become more apparent, with reference to embodiments the present invention is carried out further
Describe in detail.It should be appreciated that specific embodiment described herein is not used to limit this only to explain the present invention
It is bright.
In following examples, the Milk Thistle extract for being used is as obtained by prepared by following steps：
Dry Milk Thistle is placed in into 90% methanol or ethanol solution that flash extracter adds 6-8 times of volume, 1-3 is processed
Minute, after solid-liquid separation, reclaim methanol or ethanol solution, concentration so that extract is in paste, then with water-saturated n-butanol pair
Extract is extracted 3 times, after recycling design, using titanium rod filter concentrate drying, obtains Milk Thistle extract.
The Herba Dendrobii extract for being used is as obtained by prepared by following steps：
S1, the Herba Dendrobii that drains will be cleaned it is placed in steam-explosion jar, it is 0.6- to be first passed through nitrogen to steam explosion pressure inside the tank
1.4MPa, explosion treatment 8-25min；Then it is 1.4-1.8MPa to be passed through rapidly steam to steam explosion pressure inside the tank, at steam explosion
After reason 0.5-2.5min, relative density 1.10-1.20 is concentrated into using titanium rod filter room temperature, is spray-dried concentrated solution, obtains powder
Body；
S2, in powder body add 5-9 times of water, add by cellulase：Neutral protease, flavor protease in mass ratio 1:
The compound enzyme of 2: 3 mixing gained, is combined 3-5% of the enzyme dosage for powder body weight, 1-2h is digested at 25-35 DEG C, filter, must digest
Liquid；
S3, by naturally to thaw again after the fermentation liquid quick freeze of gained, make age of starch precipitation in extract；Collect supernatant
Liquid, remainder centrifugation are collected centrifugal liquid and are incorporated in supernatant；
S4, by supernatant with the flow velocity of 3-7BV/h by macroporous resin column, after dynamic adsorption saturation, deionized water with
The above-mentioned macroporous resin column of flow velocity drip washing of 5-8BV/h is colourless to effluent, then with the organic solvent that volume fraction is 50%-70%
Eluting is carried out with the flow velocity of 8-12BV/h, eluent is collected, concentrating under reduced pressure obtains Herba Dendrobii extract.
The Nostoc lyophilized powder for being used is as obtained by prepared by following steps：
Take, lyophilization obtains Nostoc lyophilized powder；Q wherein,
First time explosion treatment keeps pressure for 0.6-0.9Mpa, temperature 65-95 degree Celsius, time 7-23min, second explosion treatment
Holding pressure be 0.9-1.1Mpa, temperature 95-115 degree Celsius, time 0.8-1.2min, third time explosion treatment keep pressure be
3-5Mpa, temperature 70-90 degree Celsius, time 0.8-2.8min.
The Olive leaf P.E for being used is as obtained by prepared by following steps：
Will Folium olive clean after be placed in steam-explosion jar, it is 1.5-2.1MPa to be passed through nitrogen to steam explosion pressure inside the tank, and steam is quick-fried
After tear reason 0.8-2.8min, after being cooled to room temperature, alkaline protease is added by the 1-2% of raw material weight, be maintained at 25-35 DEG C
Lower enzymolysis 0.5-2h, filters, obtains enzymolysis solution；By enzymolysis solution directly by neutral alumina column, resin column blade diameter length ratio is 1: 5-1:
10, respectively with 1-4 times of cylinder hydrops, 1-3 times of 95% ethanol solution eluting remove impurity of column volume, with the 0.3- of 5-7 times of column volume
0.6% glacial acetic acid -55% ethanol (1: 1) eluant solution, collects eluent；It is after flinging to the glacial acetic acid and ethanol in eluent, micro-
Ripple is vacuum dried, and obtains Olive leaf P.E.
The grape fermentation powder for being used is as obtained by prepared by following steps：
After Fructus Vitis viniferae is cleaned after destemming, broken skin, enter in container, container is sealed with tin foil, after 30h, add Saccharum Sinensis Roxb. 2-3
Part, preservative film being covered after stirring evenly, stirs secondary daily, Pericarpium Vitis viniferae is pressed in juice, nitrogen is passed through after 7d to container inner pressure is
0.7-1.5MPa, processes 8-25min；Then be passed through rapidly steam to container inner pressure be 1.5-2.5Mpa, be cooled to room temperature by
According to the ratio microbe inoculation leaven of inoculum concentration 1.5-1.7%, keeping temperature 25-35 DEG C, after fermentation 48-54h, high temperature goes out
Living, oven drying at low temperature obtains grape fermentation powder；Wherein, microbe leaven is by lactobacilluss, Bacillus licheniformis, glutamic acid bar
The mixing gained of bacterium in mass ratio 1: 3: 2.
By obtained by lyophilization after Fructus Rubi micronizing, other medical materials for being used are city to the Herba Duchesneae Indicae lyophilized powder for being used
Sell.
Embodiment 1
A kind of Chinese medicine preparation for treating tumor, is prepared from by the component of following weight portion：
15 parts of Radix Sophorae Tonkinensiss, 10 parts of Milk Thistle extract, 10 parts of Fructus Jujubae leaf, 8 parts of Mussaenda, 12 parts of Herba Dendrobii extract, green calyx
15 parts of prunus mume (sieb.) sieb.et zucc., 12 parts of Nostoc lyophilized powder, 5 parts of Semen Caryae Cathayensis bark, 10 parts of Olive leaf P.E, 10 parts of grape fermentation powder, hop 7
Part, 5 parts of Rhizoma et radix valerianae, 10 parts of Concha Ostreae, 13 parts of Herba Scutellariae Barbatae, 5 parts of Herba Duchesneae Indicae lyophilized powder, 10 parts of Radix Et Caulis Acanthopanacis Senticosi, 10 parts of the Radix Astragali.
Embodiment 2
A kind of Chinese medicine preparation for treating tumor, is prepared from by the component of following weight portion：
25 parts of Radix Sophorae Tonkinensiss, 15 parts of Milk Thistle extract, 15 parts of Fructus Jujubae leaf, 14 parts of Mussaenda, 18 parts of Herba Dendrobii extract, green calyx
20 parts of prunus mume (sieb.) sieb.et zucc., 18 parts of Nostoc lyophilized powder, 15 parts of Semen Caryae Cathayensis bark, 15 parts of Olive leaf P.E, 15 parts of grape fermentation powder, hop
13 parts, 10 parts of Rhizoma et radix valerianae, 20 parts of Concha Ostreae, 27 parts of Herba Scutellariae Barbatae, 10 parts of Herba Duchesneae Indicae lyophilized powder, 20 parts of Radix Et Caulis Acanthopanacis Senticosi, 20 parts of the Radix Astragali.
Embodiment 3
A kind of Chinese medicine preparation for treating tumor, is prepared from by the component of following weight portion：
20 parts of Radix Sophorae Tonkinensiss, 12.5 parts of Milk Thistle extract, 12.5 parts of Fructus Jujubae leaf, 11 parts of Mussaenda, 15 parts of Herba Dendrobii extract,
17.5 parts of FLOS MUME, 15 parts of Nostoc lyophilized powder, 10 parts of Semen Caryae Cathayensis bark, 12.5 parts of Olive leaf P.E, grape fermentation powder
12.5 parts, it is 10 parts of hop, 7.5 parts of Rhizoma et radix valerianae, 15 parts of Concha Ostreae, 20 parts of Herba Scutellariae Barbatae, 7.5 parts of Herba Duchesneae Indicae lyophilized powder, 15 parts of Radix Et Caulis Acanthopanacis Senticosi, yellow
15 parts of stilbene..
The substantial amounts for the treatment of case practices of Jing, the anti-tumor agent obtained by the present embodiment have detoxicating and resolving stagnation of pathogens, blood circulation promoting and blood stasis dispelling, benefit
Gas yin nourishing, kills cancerous cell, anticancer growth, symptom management development, the effect for improving immunologic function, and therapeutic effect shows
Write, and have no that patient has untoward reaction, be adapted to patient's life-time service.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of Chinese medicine preparation for treating tumor, it is characterised in that be prepared from by the component of following weight portion：
Radix Sophorae Tonkinensiss 15-25 parts, Milk Thistle extract 10-15 parts, Fructus Jujubae leaf 10-15 parts, Mussaenda 8-14 parts, Herba Dendrobii extract
12-18 parts, FLOS MUME 15-20 part, Nostoc lyophilized powder 12-18 parts, Semen Caryae Cathayensis bark 5-15 parts, Olive leaf P.E 10-15
Part, grape fermentation powder 10-15 parts, hop 7-13 parts, Rhizoma et radix valerianae 5-10 parts, Concha Ostreae 10-20 parts, Herba Scutellariae Barbatae 13-27 parts, Herba Duchesneae Indicae are frozen
Dry powder 5-10 parts, Radix Et Caulis Acanthopanacis Senticosi 10-20 parts, Radix Astragali 10-20 parts.
2. a kind of Chinese medicine preparation for treating tumor as claimed in claim 1, it is characterised in that by the component system of following weight portion
It is standby to form：
15 parts of Radix Sophorae Tonkinensiss, 10 parts of Milk Thistle extract, 10 parts of Fructus Jujubae leaf, 8 parts of Mussaenda, 12 parts of Herba Dendrobii extract, FLOS MUME 15
Part, 12 parts of Nostoc lyophilized powder, 5 parts of Semen Caryae Cathayensis bark, 10 parts of Olive leaf P.E, 10 parts of grape fermentation powder, 7 parts of hop,
5 parts of Rhizoma et radix valerianae, 10 parts of Concha Ostreae, 13 parts of Herba Scutellariae Barbatae, 5 parts of Herba Duchesneae Indicae lyophilized powder, 10 parts of Radix Et Caulis Acanthopanacis Senticosi, 10 parts of the Radix Astragali.
3. a kind of Chinese medicine preparation for treating tumor as claimed in claim 1, it is characterised in that by the component system of following weight portion
It is standby to form：
25 parts of Radix Sophorae Tonkinensiss, 15 parts of Milk Thistle extract, 15 parts of Fructus Jujubae leaf, 14 parts of Mussaenda, 18 parts of Herba Dendrobii extract, FLOS MUME 20
Part, 18 parts of Nostoc lyophilized powder, 15 parts of Semen Caryae Cathayensis bark, 15 parts of Olive leaf P.E, 15 parts of grape fermentation powder, hop 13
Part, 10 parts of Rhizoma et radix valerianae, 20 parts of Concha Ostreae, 27 parts of Herba Scutellariae Barbatae, 10 parts of Herba Duchesneae Indicae lyophilized powder, 20 parts of Radix Et Caulis Acanthopanacis Senticosi, 20 parts of the Radix Astragali.
4. a kind of Chinese medicine preparation for treating tumor as claimed in claim 1, it is characterised in that by the component system of following weight portion
It is standby to form：
20 parts of Radix Sophorae Tonkinensiss, 12.5 parts of Milk Thistle extract, 12.5 parts of Fructus Jujubae leaf, 11 parts of Mussaenda, 15 parts of Herba Dendrobii extract, green calyx
17.5 parts of prunus mume (sieb.) sieb.et zucc., 15 parts of Nostoc lyophilized powder, 10 parts of Semen Caryae Cathayensis bark, 12.5 parts of Olive leaf P.E, 12.5 parts of grape fermentation powder,
10 parts of hop, 7.5 parts of Rhizoma et radix valerianae, 15 parts of Concha Ostreae, 20 parts of Herba Scutellariae Barbatae, 7.5 parts of Herba Duchesneae Indicae lyophilized powder, 15 parts of Radix Et Caulis Acanthopanacis Senticosi, 15 parts of the Radix Astragali.
5. a kind of Chinese medicine preparation for treating tumor as claimed in claim 1, it is characterised in that the Milk Thistle extract passes through
Following steps prepare gained：
Dry Milk Thistle is placed in into 90% methanol or ethanol solution that flash extracter adds 6-8 times of volume, 1-3 point is processed
Clock, after solid-liquid separation, reclaims methanol or ethanol solution, concentration so that extract is in paste, then with water-saturated n-butanol to carrying
Take thing to extract 3 times, after recycling design, using titanium rod filter concentrate drying, obtain Milk Thistle extract.
6. a kind of medicine for aiding in chemoembolization of hepatic carcinoma according to claim 1, it is characterised in that the Herba Dendrobii is extracted
Thing is as obtained by prepared by following steps：
S1, the Herba Dendrobii that drains will be cleaned it is placed in steam-explosion jar, it is 0.6-1.4MPa to be first passed through nitrogen to steam explosion pressure inside the tank,
Explosion treatment 8-25min；Then it is 1.4-1.8MPa, Steam explosion treatment 0.5- to be passed through rapidly steam to steam explosion pressure inside the tank
After 2.5min, relative density 1.10-1.20 is concentrated into using titanium rod filter room temperature, is spray-dried concentrated solution, obtains powder body；
S2, in powder body add 5-9 times of water, add by cellulase：Neutral protease, flavor protease in mass ratio 1: 2: 3
The compound enzyme of mixing gained, is combined 3-5% of the enzyme dosage for powder body weight, 1-2h is digested at 25-35 DEG C, filter, obtain enzymolysis solution；
S3, by naturally to thaw again after the fermentation liquid quick freeze of gained, make age of starch precipitation in extract；Collect supernatant,
Remainder is centrifuged, and collects centrifugal liquid and is incorporated in supernatant；
S4, by supernatant with the flow velocity of 3-7BV/h by macroporous resin column, after dynamic adsorption saturation, deionized water is with 5-
The above-mentioned macroporous resin column of flow velocity drip washing of 8BV/h is colourless to effluent, then with the organic solvent that volume fraction is 50%-70% with
The flow velocity of 8-12BV/h carries out eluting, collects eluent, and concentrating under reduced pressure obtains Herba Dendrobii extract.
7. a kind of Chinese medicine preparation for treating tumor as claimed in claim 1, it is characterised in that the Nostoc lyophilized powder passes through
Following steps prepare gained：
Take, lyophilization obtains Nostoc lyophilized powder；Q wherein, first
Separate explosion process keeps pressure for 0.6-0.9Mpa, temperature 65-95 degree Celsius, time 7-23min, and second explosion treatment keeps
Pressure is 0.9-1.1Mpa, temperature 95-115 degree Celsius, time 0.8-1.2min, and it is 3- that third time explosion treatment keeps pressure
5Mpa, temperature 70-90 degree Celsius, time 0.8-2.8min.
8. a kind of Chinese medicine preparation for treating tumor as claimed in claim 1, it is characterised in that the Olive leaf P.E passes through
Following steps prepare gained：
It is placed in steam-explosion jar after Folium olive is cleaned, it is 1.5-2.1MPa to be passed through nitrogen to steam explosion pressure inside the tank, at steam explosion
After reason 0.8-2.8min, after being cooled to room temperature, alkaline protease, enzyme at being maintained at 25-35 DEG C are added by the 1-2% of raw material weight
Solution 0.5-2h, filters, obtains enzymolysis solution；By enzymolysis solution directly by neutral alumina column, resin column blade diameter length ratio is 1: 5-1: 10, point
Not with 1-4 times of cylinder hydrops, 1-3 times of 95% ethanol solution eluting remove impurity of column volume, with the 0.3-0.6% ice of 5-7 times of column volume
Acetic acid -55% ethanol (1: 1) eluant solution, collects eluent；After flinging to the glacial acetic acid and ethanol in eluent, microwave vacuum is done
It is dry, obtain Olive leaf P.E.
9. a kind of Chinese medicine preparation for treating tumor as claimed in claim 1, it is characterised in that the grape fermentation powder by with
Lower step prepares gained：
After Fructus Vitis viniferae is cleaned after destemming, broken skin, enter in container, container is sealed with tin foil, Saccharum Sinensis Roxb. 2-3 parts are added after 30h, is stirred
Preservative film is covered after even, stirs secondary daily, Pericarpium Vitis viniferae is pressed in juice, it is 0.7- that nitrogen to container inner pressure is passed through after 7d
1.5MPa, processes 8-25min；Then it is 1.5-2.5Mpa to be passed through rapidly steam to container inner pressure, is cooled to room temperature according to connecing
The ratio microbe inoculation leaven of amount 1.5-1.7% is planted, keeping temperature 25-35 DEG C is fermented after 48-54h, and high-temperature inactivation is low
Temperature drying, obtains grape fermentation powder.
10. a kind of Chinese medicine preparation for treating tumor as claimed in claim 8, it is characterised in that the microbe leaven by
The mixing gained of lactobacilluss, Bacillus licheniformis, Corynebacterium glutamicum in mass ratio 1: 3: 2.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106860768A (en) * | 2017-04-13 | 2017-06-20 | 河南中医药大学 | A kind of Chinese medicine compound prescription for treating the cancer of the esophagus |
| CN107050128A (en) * | 2017-05-09 | 2017-08-18 | 江苏护理职业学院 | A kind of antitumor giant knotweed compound preparation and preparation method thereof |
| CN109793877A (en) * | 2019-04-03 | 2019-05-24 | 赤峰市肿瘤医院(赤峰学院第二附属医院) | A kind of Chinese medicine composition for treating tumour |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105920194A (en) * | 2016-04-24 | 2016-09-07 | 新乡医学院 | Anti-tumor traditional Chinese medicinal agent |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105920194A (en) * | 2016-04-24 | 2016-09-07 | 新乡医学院 | Anti-tumor traditional Chinese medicinal agent |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106860768A (en) * | 2017-04-13 | 2017-06-20 | 河南中医药大学 | A kind of Chinese medicine compound prescription for treating the cancer of the esophagus |
| CN107050128A (en) * | 2017-05-09 | 2017-08-18 | 江苏护理职业学院 | A kind of antitumor giant knotweed compound preparation and preparation method thereof |
| CN109793877A (en) * | 2019-04-03 | 2019-05-24 | 赤峰市肿瘤医院(赤峰学院第二附属医院) | A kind of Chinese medicine composition for treating tumour |
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Application publication date: 20170412 |