CN107041852A - A kind of cosmetics for removing face mite - Google Patents

Abstract

The invention discloses a kind of cosmetics, the cosmetics are made up of active peptides and mixed-matrix；Wherein, mixed-matrix weight ratio is：Vaseline: lanolin: sodium tetraborate=10 13: 24: 1；The ratio between active peptides and mixed-matrix gross weight are 1: 200 250.The cosmetics that the present invention is prepared have preferable mite killing worm effect, while the characteristics of also having natural, gentle, non-stimulated to skin, its effect is better than ordinary cosmetics, in the whitening of skin, also all has preferable effect in moisturizing, is suitable for promoting the use of.

Description

A kind of cosmetics for removing face mite

Technical field

The present invention relates to a kind of cosmetics, and in particular to a kind of efficiently to remove the cosmetics of face mite.

Background technology

In recent years research is thought：Human body skin acarid and human body face produced problem：Such as epifolliculitis, seborrhea, Alopecia, blear-eye, external auditory meatus itch etc., it is particularly and acne, being related for brandy nose is very close.Acarid multiparasitization is in hair In capsule sebaceous glands, absorption intracellular nutritional material, and smegma thing, and damaged normal cell；And due to the machinery of polypide Stimulate and the chemical irritation of polypide excreta is acted on, parasitic site can cause hair follicle to expand, blood vessel dilatation peripheral cell infiltrates, Proliferation of fibrous tissue, and make tissue damage, cause allergic reaction, red grouper, papule, granuloma, warts and itch occurs in skin Etc. phenomenon.Acne is also known as ' acne ', ' acne ' etc. in the traditional Chinese medical science, among the people to be commonly called as ' dark sore ' or ' whelk ', associated with acarid. It is commonly considered as puberty men and women's conception vessel to lead to, taichong channel is contained, kidney qi is full and qi and blood is prosperous, does not save if diet is greasy, or seven feelings are too drastic, Kidney qi can be caused vigorous, damp heat in the spleen and the stomach, liver and gall stagnated fire, punching appoints the stasis of blood stagnant, gas fire phlegm-blood stasis is followed and done through upper, it is then acne to send out in fur. Facial vermiform mite active degree is with mood, diet, health and menstrual cycle fluctuations.Again because damp and hot stream connects, phlegm stagnation in collateral, Therefore acne, the more difficult speed of epifolliculitis such as brandy nose is cured.At present, medically treat main based on mite killing, with most chemicalses Combination carries out mite killing treatment, can all receive certain effect.

A kind of ointment for treating face mite disease in CN 1091369A, it is using sublimed sulfur, metronidazole, chloroquine, flutterred The quick, vitamin C of that, the fine powder of vitamin E are raw material, and it is mixed base to add Suitable melting vaseline, lanolin, sodium tetraborate Matter, mixes the ointment being made.Although the ointment can partly kill acarid, to the face acne caused by mite infection, acne The diseases such as sore, papillule shape hyperplasia, maculopapule, myxedema, acne rosacea, also there is obvious curative effects, but formula is complicated, and The course for the treatment of is longer, still there is larger room for improvement.

CN 102357157A disclose a kind of Chinese medicine composition for treating acne with mite infection.It is directed to pure Acne, is made up of following Chinese medicines, the red sage root, American Ginseng, honeysuckle, Ligusticum wallichii, the root of herbaceous peony, safflower, radix ampelopsis, rattlebush, Gorgon fruit, the root of Dahurain angelica, Rhodiola root, golden cypress, rhizoma nelumbinis.If patient has pet close contact history, so that skin is wiped quality testing and tested, acarid and other cause of diseases are found Body, is suitable on the basis of above-mentioned formula, moreover it is possible to further plus with the coptis, kuh-seng, the tuber of stemona, fructus schisandrae, the Radix Astragali, coix seed, white puncture Lamb's-quarters, the fruit of Chinese wolfberry, asarum, are together decocted, and are filtered its decoction, are warmly taken.The present invention goes out using pure Chinese medicine as raw material from theory of traditional Chinese medical science Hair, with conditioning viscera, Yin Yang balancing, irritability of relaxing, promoting blood circulation, changes blood stasis, desinsection except mite, eradicates acne, skin-protecting face nursing and anti- The effect of only recurring.This method also because method is complicated, and important component is numerous, to the lesions of liver and kidney of human body also compared with Greatly, curative effect, slowly, and is unsuitable for promoting the use of on a large scale.

At present, the cosmetics species of in the market is various, such as cosmetic cream, beauty cream, fair complexion cream, mildy wash, this kind of U.S. Hold cosmetics production technology and formula be essentially all it is similar, also it is different degrees of to human body skin have protection beauty kill The effect of bacterium, but effect is not fine, the effect for also all not having nursing for treating to face acnes, whelk caused by acarid. Therefore, except mite cosmetics that are efficient, lasting and being had no side effect to human body are found, it has also become the trend of current cosmetic area research.

The content of the invention

It is an object of the invention to provide a kind of except mite cosmetics, the thing that the active component of the cosmetic is matched by following weight parts Matter is constituted：Acaricidal activity peptide and mixed-matrix composition；Wherein, mixed-matrix weight ratio is：Vaseline: lanolin: sodium tetraborate =10-13: 2-4: 1；The ratio between acaricidal activity peptide and mixed-matrix gross weight are 1: 200-250.

Still further aspect of the present invention is there is provided a kind of acaricidal activity peptide, its sequence such as SEQ ID NO：Shown in 1-21 is any.

Another object of the present invention is to provide a kind of cosmetics for treating face mite, and it can thoroughly kill acarid, To diseases such as various acne rosaceas, acne, the acne caused by mite infection, there are obvious curative effects, its cosmetic quality being made Amount is stable, easy to use, non-stimulated to skin, have protection, softening function.

Cosmetics of the present invention can be used as preparing product after suncream, sunlight lotion, solarization, day cream, late frost, facial mask, skin cream, wash Face milk, skin toner powder etc., above-mentioned product technology as known to the industry are manufactured.Be particularly useful for making creme, emulsion, gel, Facial mask paste.

The beneficial effects of the invention are as follows:

The cosmetics of the offer of the present invention being capable of significant except mite function.The present invention uses acaricidal activity peptide to be prepared for raw material Obtained cosmetics, it is natural, gentle, non-stimulated to skin, and act on skin and can significantly kill acarid, its effect will The except mite product conventional better than prior art.

The detailed description of preferred embodiment

Following example illustrate each aspect of the present invention content.They limit the model of the present invention only to illustrate, not Enclose.

The preparation of the acaricidal activity peptide of embodiment 1

Applicant's early stage is by library screening, the active peptide with mite killing function of acquisition, and its sequence is respectively such as SEQ ID NO：Shown in 1-21, each polypeptide title corresponds to SMT-1~21.Artificial synthesized method is respectively adopted, described polypeptide is obtained (in later stage mass production, it would however also be possible to employ the method for the conventional prokaryotic expression in this area is produced).Each is more Peptide is dissolved using sterilized water, prepares standby as 120ppm concentration.

The acaricidal activity peptide compliance test result of embodiment 2

(1) acquisition and culture of acarid

(1) carried out taking acarid at positions such as cheek, foreheads with medical quantitative mite extracting apparatus.(2) acarid will be contained with toothpick Sebum secretion thing takes out from acari taking device, is put into the plate equipped with long patent flour, 28 degrees Celsius, 80% humidity culture 25 My god, you can the prolific acarid of harvest.

(2) acaricidic experiment

By acarid according to the similar average out to 23*3 groups of vigor, every group 20,3 are repeated 3 times to be parallel；Every group of sample point It is not coated on 120ppm polypeptide on slide；The polypeptide of each group one species of correspondence；And ensure each experiment Conditions Temperature and humidity all same.Polypeptide is set fully to be combined with acarid, covered.Ensure that every part of sample is used during observation same One microscope.The situation (active situation, the gnathosoma of acarid, and without motion completely) of polypide is recorded under high power lens.Work as polypide When completely inactive, as acarid is dead.Every group of each polypide death condition of 30min observed and recordeds.It is necessary during observation every time Keep experiment condition temperature and humidity consistent..Using SPSS19.0 statistics softwares, statistical analysis, measurement data are carried out Examined using t, enumeration data is handled using Chi-square Test, with P<0.05 is to have significant difference.Feminine gender is used as using sterilized water group Control, positive control is used as using the preparation in CN1247227C.As a result it is as shown in table 1：

From the results shown in Table 1, after dosing, the 1-21 group acarid death rates compare, with the extension of administration timing of drug, There is rise trend in this 21 groups of acarid death rates, and basic polypide is all dead in 60min, and dead required time is shorter.Nothing Bacterium water control group polypide is without death, positive controls, the death rate also height without the application after 60min.This absolutely proves, The mite killing polypeptide of the present invention is compared with other groups, and there were significant differences, the statistically significant (P of difference<0.05).

The preparation of the cosmetics of embodiment 3

By SEQ ID NO：1 i.e. SMT-1 mite killing polypeptides are added to the lanolin 90 melted in advance, are added after mixing in advance The vaseline of fusing.The uniform rear sodium tetraborate for adding dissolving to be mixed, and add the appropriate tune selected according to actual needs Taste agent essence, finally stirs, after cooling ointment.Wherein vaseline: lanolin: sodium tetraborate=10: 2: 1, polypeptide Addition be：The ratio between acaricidal activity peptide and mixed-matrix gross weight are 1: 250.Mixed-matrix is vaseline, lanolin, four boron The quality summation of sour sodium.

The preparation of cosmetics SMT-2~21 of embodiment 4

According to the method for embodiment 2, polypeptide is replaced with to SMT-2~21 totally 20 kinds of forms, remaining component and preparation respectively Method and embodiment 1 are identical, prepare the cosmetics.

The security verification of embodiment 5

In order to evaluate the security of cosmetics of the invention, design skin below acute toxicity test 300 grams of body weight of selection Left and right, skin smooth, not damaged, without dermopathic cavy 84, is divided into 21 groups, 4 repetitions of each polypeptide cosmetic correspondence.Globefish Mouse is in tested preceding 24 hours backs both sides unhairing 30cm².Wherein epidermis is scratched in side with sterile surgical knife work " well " font, with skin It is degree that slight oozing of blood, which occurs, in skin.1 gram of cosmetics of the invention is directly applied to plucked complete and damaged skin.After tested material 24 hours, it there are no and be poisoned to death.Continue to observe, continuous seven days, all animal subject outward appearance behaviors, abnormal motion, spiritual shape State, appetite is defecated, the result that the no abnormal of index such as breathing is tested according to above skin sensibiligen, can point out this The cosmetics of invention are without acute dermal toxicity, with higher security.

The cosmetic effect of embodiment 6 is detected

By cooperating with dept. of dermatology of the first affiliated hospital of Xi'an Communications University, 69 clinics of selection and laboratory diagnosis are mite property Patient's (average age 23.7 years old) of dermatitis participate in this 1 week by a definite date it is random, control clinical treatment experiment control groups use CN1247227C externally used compound Liposomal formulation, the cosmetics that 1-21 groups are prepared using the present invention in addition, not contain The cosmetics of the invention of antibacterial peptide are used as blank control.The patient for carrying out clinical observation enters this and treated before January, equal nothing System and local medication history.69 patients follow the doctor's advice, after daily morning and evening face is cleaned with neutral soap, after warm water cleaning, The cosmetics of the present invention are coated in face, daily once in the morning and once at night.By to facial inflammatory lesion skin damaged, non-inflammatory lesions skin Damage progress to score classification, as a result find, after medication end in one week, the skin damaged that is averaged decline rate is as shown in table 2 below：

From the results shown in Table 2, cosmetics of the invention have preferable skin damaged decline rate, and compared with the control Effect has significant difference (p<0.05).Find simultaneously the present invention for the facial skin damaged of inflammatory, clinical observation material proves this hair Bright cosmetics are better than current common dosage forms, and safe, nontoxic pair in disease of skin caused by treatment dragonfly worm skin infection Effect.

Take acarid to test in addition, carrying out surface skin for the patient treated after one week, use the trouble of cosmetics of the present invention Person's surface skin is substantially not detectable the presence of acarid, and this absolutely proves that mite killing worm effect is notable.And patient's skin of control formulation Skin surface remains a certain amount of acarid, continues to be treated using the preparation, and at 3 weeks, surface skin was detected not To the presence of the acarid.

Above content is to combine specific preferred embodiment Jin ー steps made for the present invention to describe in detail, it is impossible to assert The specific implementation of the present invention is confined to these explanations.For general technical staff of the technical field of the invention, On the premise of not departing from present inventive concept, some simple deduction or replace can also be made, should all be considered as belonging to the present invention's Protection domain.

Sequence table

The ＞ of ＜ 110 turn round and look at silver-colored phoenix

A kind of cosmetics of the ＞ of ＜ 120

〈160〉21

〈210〉1

〈211〉26

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-1

PGPYVPQHMQQTGRPTVWTFIDSSIHH

〈210〉2

〈211〉26

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-2

IYMRTSSIKWCTARGENHNNQGNNWMS

〈210〉3

〈211〉25

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-3

MHCIMMYWRSVHHSATLSIHFRWSNR

〈210〉4

〈211〉25

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-4

IQTLPHHCHSMGHPWLFHFLSSWNNH

〈210〉5

〈211〉26

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-5

RGTQNTVEHVQYRDEWAAYSQHHAVFW

〈210〉6

〈211〉24

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-6

WHSYRNCPVSWFHMYKTWPPYDRTM

〈210〉7

〈211〉24

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-7

GCPLDFPADQSTHPNFMPCEVRLVE

〈210〉8

〈211〉25

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-8

ATERRAPRHPMDGASPWACHSAQNQP

〈210〉9

〈211〉26

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-9

NTKDMMDSSSAYVPQFYAISAFWGIEM

〈210〉10

〈211〉25

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-10

NLCYHPQFGDKMWQAWPYWSRDKAVN

〈210〉11

〈211〉26

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-11

HQRWDQTIRHELTRYYTPVQGIGYTPA

〈210〉12

〈211〉25

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-12

VAHWQAHTFKIYDVSSHDWANWQQQS

〈210〉13

〈211〉26

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-13

IWMSWGKYQAFMMQKMLWTYANRNQLG

〈210〉14

〈211〉25

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-14

RRVVIRGFSGMASGDLVEQQSEGTIP

〈210〉15

〈211〉26

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-15

IWYSNTDVEQDLPEHIDCAHCCNWKPY

〈210〉16

〈211〉25

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-16

SRVDTGSMYLSPSSQAPWHQCVFDLL

〈210〉17

〈211〉25

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-17

GHQQENIIHYCQGTYVDRFPTDPTWP

〈210〉18

〈211〉25

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-18

AAEILNPFFKCPFICPKILTWIPYKM

〈210〉19

〈211〉26

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-19

HVSVQCYWSARHCHIQIHRFRTPIEDG

〈210〉20

〈211〉26

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-20

NAKRIKWWCEVFPRAPKFFKDIDCEKP

〈210〉21

〈211〉24

〈212〉PRT

The ＞ artificial sequences of ＜ 213

〈400〉SMT-21

EHEPHHSEQQWGTRVVVSENWTWKN

Claims (5)

1. a kind of cosmetics, it is characterised in that：With the effect of mite killing worm.

2. cosmetics as claimed in claim 1, it is characterised in that：The active component of the cosmetic is by acaricidal activity peptide and mixed base Matter is constituted；Wherein, mixed-matrix weight ratio is：Vaseline: lanolin: sodium tetraborate=10-13: 2-4: 1；Acaricidal activity peptide with The ratio between mixed-matrix gross weight is 1: 200-250.

3. cosmetics as claimed in claim 2, it is characterised in that：The acaricidal activity peptide sequence such as SEQ ID NO：1-21 Shown in any.

4. a kind of polypeptide, it is characterised in that：Its sequence such as SEQ ID NO：Shown in 1-21 is any.

5. polypeptide described in claim 4 is for preparing the application in mite killing worm cosmetics.