CN107041556A - A kind of is health products of auxiliary reducing blood lipid of raw material and preparation method thereof with wood frog head - Google Patents
A kind of is health products of auxiliary reducing blood lipid of raw material and preparation method thereof with wood frog head Download PDFInfo
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- CN107041556A CN107041556A CN201710212499.6A CN201710212499A CN107041556A CN 107041556 A CN107041556 A CN 107041556A CN 201710212499 A CN201710212499 A CN 201710212499A CN 107041556 A CN107041556 A CN 107041556A
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- Prior art keywords
- wood frog
- head
- enzyme
- polysaccharides
- reducing blood
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- 241000191896 Rana sylvatica Species 0.000 title claims abstract description 47
- 210000004369 blood Anatomy 0.000 title claims abstract description 24
- 239000008280 blood Substances 0.000 title claims abstract description 24
- 230000036541 health Effects 0.000 title claims abstract description 23
- 150000002632 lipids Chemical class 0.000 title claims abstract description 18
- 239000002994 raw material Substances 0.000 title claims abstract description 13
- 238000002360 preparation method Methods 0.000 title abstract description 7
- 238000000034 method Methods 0.000 claims abstract description 22
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 21
- 239000005017 polysaccharide Substances 0.000 claims abstract description 21
- 150000004676 glycans Chemical class 0.000 claims abstract description 20
- 235000004347 Perilla Nutrition 0.000 claims abstract description 16
- 241000229722 Perilla <angiosperm> Species 0.000 claims abstract description 16
- 240000007087 Apium graveolens Species 0.000 claims abstract description 12
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 claims abstract description 12
- 235000010591 Appio Nutrition 0.000 claims abstract description 12
- 239000000843 powder Substances 0.000 claims abstract description 12
- URQMEZRQHLCJKR-UHFFFAOYSA-N 3-Methyl-5-propyl-2-cyclohexen-1-one Chemical compound CCCC1CC(C)=CC(=O)C1 URQMEZRQHLCJKR-UHFFFAOYSA-N 0.000 claims abstract description 4
- 102000004190 Enzymes Human genes 0.000 claims description 18
- 108090000790 Enzymes Proteins 0.000 claims description 18
- 239000000284 extract Substances 0.000 claims description 6
- 238000001556 precipitation Methods 0.000 claims description 6
- 238000002525 ultrasonication Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 239000012488 sample solution Substances 0.000 claims description 5
- 108091005804 Peptidases Proteins 0.000 claims description 4
- 239000004365 Protease Substances 0.000 claims description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 4
- 239000003513 alkali Substances 0.000 claims description 4
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 238000005238 degreasing Methods 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000000746 purification Methods 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 238000007873 sieving Methods 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 238000005119 centrifugation Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 13
- 238000002604 ultrasonography Methods 0.000 abstract description 5
- 230000036772 blood pressure Effects 0.000 abstract description 4
- 238000001035 drying Methods 0.000 abstract description 3
- 210000000988 bone and bone Anatomy 0.000 abstract 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 6
- 229910052791 calcium Inorganic materials 0.000 description 6
- 239000011575 calcium Substances 0.000 description 6
- 238000000605 extraction Methods 0.000 description 5
- 238000012545 processing Methods 0.000 description 5
- 230000000055 hyoplipidemic effect Effects 0.000 description 3
- 108010023302 HDL Cholesterol Proteins 0.000 description 2
- 241000700159 Rattus Species 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000003101 oviduct Anatomy 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 150000003626 triacylglycerols Chemical class 0.000 description 2
- 208000008454 Hyperhidrosis Diseases 0.000 description 1
- 108010028554 LDL Cholesterol Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 208000013219 diaphoresis Diseases 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- AZHSSKPUVBVXLK-UHFFFAOYSA-N ethane-1,1-diol Chemical compound CC(O)O AZHSSKPUVBVXLK-UHFFFAOYSA-N 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000009200 high fat diet Nutrition 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 108010022197 lipoprotein cholesterol Proteins 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Sustainable Development (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a kind of health products and preparation method thereof for aiding in reducing blood lipid for raw material with wood frog head, wherein, preparation method comprises the following steps：(1) Rana sylvatica Le conte bone is digested using ultrasound-assisted enzymolysis method, obtains Polysaccharides；(2) by mountain celery and purple perilla drying, crushing, powder is made；(3) by Polysaccharides, mountain celery powder and purple perilla powder with 1：2：2 mass ratio is well mixed, and produces this product.The present invention is advantageous in that：Mountain celery and purple perilla contain a variety of nutriments, and the effects such as have lowering blood pressure and blood fat, are combined together with Polysaccharides, three can cooperate with promotion reducing blood lipid, so the health products of the present invention can play good effect for reducing blood fat, and effect is notable.
Description
Technical field
The present invention relates to a kind of health products of auxiliary reducing blood lipid and preparation method thereof, and in particular to a kind of is original with wood frog head
Health products of auxiliary reducing blood lipid of material and preparation method thereof, belong to health product technology field.
Background technology
Wood frog is a kind of to integrate edible, medicinal, health care rare frog kind.
Wood frog head is the discarded object in oviductus ranae process, and yield is big, using few.At present, wood frog converted products is main
Based on oviductus ranae, and the utilization to the wood frog after stripping oil and the male frog is still seldom, just less to the processing and utilization of wood frog head,
Only functional study on wood frog head is also concentrated mainly on anticoagulation.
The health products species of auxiliary reducing blood lipid class on the market is various, and does not have with wood frog head for the health products of primary raw material
Have.
Therefore, with wood frog head for primary raw material, the comprehensive of wood frog can be improved by developing a kind of health products that can aid in reducing blood lipid
Utilization rate is closed, increases the added value of wood frog, the development tool of wood frog industry is of great significance.
The content of the invention
It is an object of the invention to provide a kind of health products with wood frog head for the auxiliary reducing blood lipid of raw material and its preparation side
Method.
In order to realize above-mentioned target, the present invention is adopted the following technical scheme that：
A kind of method that the health products for aiding in reducing blood lipid are prepared for raw material with wood frog head, it is characterised in that including following step
Suddenly：
Step1：Extract Polysaccharides
Wood frog takes head, dries, by the crushing of dried wood frog head, degreasing, weighs a certain amount of wood frog head powder, then presses
1：10 material liquid volume is than addition distilled water, first ultrasonication, then wood frog head is digested using alkali protease, digests
Condition be：Enzyme concentration 2wt%, 9.5 are adjusted to by pH, and the sample solution for having adjusted pH is put into 55 DEG C of water-bath and digested, enzyme
Solution carry out going out successively to solution after terminating enzyme, removing protein, alcohol precipitation is stayed overnight, centrifuges extract solution, collect precipitation, washing, dry
And separation and purification treatment, that is, obtain Polysaccharides；
Step2：Handle mountain celery and purple perilla
Mountain celery and purple perilla are positioned at 40 DEG C~50 DEG C relatively low temperature and dried, is then crushed, re-sieving, finally
Take powder stand-by；
Step3：Dispensing
By Polysaccharides, mountain celery powder and purple perilla powder according to 1：2：2 mass ratio is well mixed, and produces the present invention's
Health products.
Foregoing method, it is characterised in that in Step1, wood frog head is placed under conditions of 40 DEG C -50 DEG C and done
It is dry.
Foregoing method, it is characterised in that in Step1, the condition of ultrasonication is：It is ultrasonic under conditions of 300W
Ripple handles 30min.
Foregoing method, it is characterised in that in Step1, sample solution is put into the time digested in 55 DEG C of water-bath and is
4h~5h.
Foregoing method, it is characterised in that in Step1, the condition for the enzyme that goes out is：In the water-bath that solution is placed on to 95 DEG C
Progress is gone out enzyme, and the time for the enzyme that goes out is 10min-15min.
Foregoing method, it is characterised in that in Step1, centrifuges and is carried out under the conditions of 9500rpm.
The present invention is advantageous in that：
(1) using wood frog head as main raw material, wood frog processing added value can be increased substantially, wood frog raw material is improved comprehensive
Close utilization rate；
(2) wood frog head is handled using ultrasound-assisted enzymolysis method, Polysaccharides extraction conditions are gentle, recovery rate
Height, active ingredient retains more intact, and auxiliary lipid-lowering function is notable；
(3) mountain celery and purple perilla contain a variety of nutriments, and the effects such as have lowering blood pressure and blood fat, it is many with wood frog head
Sugar is combined together, and three can cooperate with promotion reducing blood lipid, so the health products of the present invention can play good reducing blood lipid and make
With, and effect is notable；
(4) wood frog collection is eaten, medicine is used for one, rich in calcium constituent, and obtained health products also have this additional work(of replenishing the calcium
Can, and side effect is smaller compared with inorganic calcium product.
Embodiment
Make specific introduce to the present invention below in conjunction with specific embodiment.
The present invention's is mainly included the following steps that with wood frog head for the method that raw material prepares the health products for aiding in reducing blood lipid：
First, Polysaccharides are extracted
We are handled wood frog head using ultrasound-assisted enzymolysis method, by single factor experiment, response surface optimization, really
The optimal extraction conditions of Polysaccharides are determined, we can obtain the recovery rate of maximum under the extraction conditions.
The process that ultrasound-assisted enzymolysis method extracts Polysaccharides is specific as follows：
Wood frog takes head on Changbai Mountain, is dried at a temperature of wood frog head is placed on into 40 DEG C, drying temperature can be properly increased,
But maximum temperature, no more than 50 DEG C, relatively low drying temperature can keep the bioactivity of required material-Polysaccharides.
By the crushing of dried wood frog head, degreasing, a certain amount of wood frog head powder is weighed, then by 1：10 material liquid volume
Than adding distilled water, then the ultrasonication 30min under conditions of 300W carries out enzyme using alkali protease to wood frog head again
Solve, the condition of enzymolysis is：Enzyme concentration 2wt% (by wood frog head powder quality than 2% add alkali protease), pH is adjusted to
9.5, the sample solution for having adjusted pH is put into 55 DEG C of water-bath and digests 4.5h, sufficient enzymolysis processing is carried out.
After enzymolysis terminates, solution is placed in 95 DEG C of water-bath and carries out the enzyme that goes out, the time for the enzyme that goes out is 10min, can be appropriate
15min is extended to, enzyme is complete to ensure to go out.
After the enzyme that goes out terminates, obtained solution decompression is concentrated into appropriate volume, then solution carried out with 95% ethanol
Alcohol precipitation is simultaneously stayed overnight, and is centrifuged extract solution under the conditions of 9500rpm afterwards, is collected precipitation, is done after being washed twice with absolute ethyl alcohol
It is dry, produce wood frog head Thick many candies.
Because the polysaccharide now obtained is mixture, wherein also many impurity, therefore we first pass through removing protein and saturating
Protein and small-molecule substance among analysis removing, then it is isolated and purified, finally give with hypolipemic function
Saccharide portion, it is standby.
We are extracted using ultrasound-assisted enzymolysis method to Polysaccharides, and not only extraction conditions are gentle, Er Qieti
Take rate high, be computed, the extraction yield of Polysaccharides is up to more than 4.3%.
In addition, we carry out zoopery by setting up High fat diet rats model, the hypolipemic function of Polysaccharides is entered
Research is gone.
Result of study is found：After administration terminates, T-CHOL (TC), triglycerides (TG) and low-density in rat blood serum
The level of lipoprotein cholesterol (LDL-C) has obvious reduction, and the content of HDL-C (HDL-C) is aobvious
Write and improve.
This explanation：Polysaccharides have significant hypolipemic function.
2nd, processing mountain celery and purple perilla
Mountain celery and purple perilla are positioned at 40 DEG C~50 DEG C relatively low temperature and dried, is then crushed, after 200 mesh
Sieve, finally takes powder stand-by.
Mountain celery has the health-care efficacy such as radiating inducing diaphoresis, damp-clearing pain-relieving, lowering blood pressure and blood fat, aid digestion.
Purple perilla is the local medicinal material in Changbai Mountain, its property and flavor of peppery and warm, have effects that to deliver, cold dispelling, qi-regulating and battalion.
We only do simple processing to mountain celery and purple perilla, and their healthcare function can be so kept to the full extent.
3rd, dispensing
By Polysaccharides, mountain celery powder and purple perilla powder according to 1：2：2 mass ratio is well mixed, and produces the present invention's
Health products.
Mountain celery and purple perilla contain a variety of nutriments, and the effects such as have lowering blood pressure and blood fat, and with having reducing blood lipid
After the Polysaccharides of effect are combined together, three can cooperate with promotion reducing blood lipid, so the health products of the present invention can rise
To good effect for reducing blood fat, and effect is notable.
Further, since wood frog is the animal of medicine-food two-purpose, rich in calcium constituent, so the present invention with wood frog head for raw material
Health products, while the health-care effect with auxiliary reducing blood lipid is ensured, also with the additional benefit replenished the calcium, and and inorganic calcium
Product is smaller compared to side effect.
It should be noted that the invention is not limited in any way for above-described embodiment, all use equivalent substitutions or equivalent change
The technical scheme that the mode changed is obtained, all falls within protection scope of the present invention.
Claims (7)
1. a kind of method that the health products for aiding in reducing blood lipid are prepared for raw material with wood frog head, it is characterised in that comprise the following steps：
Step1：Extract Polysaccharides
Wood frog takes head, dries, by the crushing of dried wood frog head, degreasing, a certain amount of wood frog head powder is weighed, then by 1：10
Material liquid volume than add distilled water, first ultrasonication, then using alkali protease to wood frog head digest, the bar of enzymolysis
Part is：Enzyme concentration 2wt%, 9.5 are adjusted to by pH, and the sample solution for having adjusted pH is put into 55 DEG C of water-bath and digested, enzymolysis knot
Solution is carried out successively after beam going out enzyme, removing protein, alcohol precipitation stay overnight, centrifuge extract solution, collect precipitation, washing, dry and
Separation and purification treatment, that is, obtain Polysaccharides；
Step2：Handle mountain celery and purple perilla
Mountain celery and purple perilla are positioned at 40 DEG C~50 DEG C relatively low temperature and dried, is then crushed, re-sieving finally takes powder
End is stand-by；
Step3：Dispensing
By Polysaccharides, mountain celery powder and purple perilla powder according to 1：2：2 mass ratio is well mixed, and produces the health care of the present invention
Product.
2. wood frog head according to the method described in claim 1, it is characterised in that in Step1, is placed on 40 DEG C -50 DEG C
Under the conditions of be dried.
3. according to the method described in claim 1, it is characterised in that in Step1, the condition of ultrasonication is：In 300W
Under conditions of ultrasonication 30min.
4. method according to claim 3, it is characterised in that in Step1, sample solution is put into enzyme in 55 DEG C of water-bath
The time of solution is 4h~5h.
5. method according to claim 4, it is characterised in that in Step1, the condition for the enzyme that goes out is：Solution is placed on
The enzyme that goes out is carried out in 95 DEG C of water-bath, the time for the enzyme that goes out is 10min-15min.
6. method according to claim 5, it is characterised in that in Step1, centrifugation is entered under the conditions of 9500rpm
OK.
What 7. a kind of method as described in claim 1 to 6 any one was prepared aids in reducing blood lipid with wood frog head for raw material
Health products.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710212499.6A CN107041556A (en) | 2017-04-01 | 2017-04-01 | A kind of is health products of auxiliary reducing blood lipid of raw material and preparation method thereof with wood frog head |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710212499.6A CN107041556A (en) | 2017-04-01 | 2017-04-01 | A kind of is health products of auxiliary reducing blood lipid of raw material and preparation method thereof with wood frog head |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107041556A true CN107041556A (en) | 2017-08-15 |
Family
ID=59544241
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710212499.6A Pending CN107041556A (en) | 2017-04-01 | 2017-04-01 | A kind of is health products of auxiliary reducing blood lipid of raw material and preparation method thereof with wood frog head |
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| CN (1) | CN107041556A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1377671A (en) * | 2001-04-03 | 2002-11-06 | 辽宁省桓仁药业有限责任公司 | Fat reducing Chinese tree frog soft capsule |
| CN1872093A (en) * | 2006-04-28 | 2006-12-06 | 崔岩 | Calcium tablets of bone of Rana chensinensis, and preparation method |
| CN103519192A (en) * | 2013-10-31 | 2014-01-22 | 蒋科富 | Frog meat food |
| CN104212862A (en) * | 2013-06-03 | 2014-12-17 | 吉林省现代中药工程研究中心有限公司 | Wood frog protein and bone calcium extraction method |
-
2017
- 2017-04-01 CN CN201710212499.6A patent/CN107041556A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1377671A (en) * | 2001-04-03 | 2002-11-06 | 辽宁省桓仁药业有限责任公司 | Fat reducing Chinese tree frog soft capsule |
| CN1872093A (en) * | 2006-04-28 | 2006-12-06 | 崔岩 | Calcium tablets of bone of Rana chensinensis, and preparation method |
| CN104212862A (en) * | 2013-06-03 | 2014-12-17 | 吉林省现代中药工程研究中心有限公司 | Wood frog protein and bone calcium extraction method |
| CN103519192A (en) * | 2013-10-31 | 2014-01-22 | 蒋科富 | Frog meat food |
Non-Patent Citations (2)
| Title |
|---|
| 姜丽冬 等: ""林蛙头多糖的提取工艺优化及其降血脂功能"", 《东北林业大学学报》 * |
| 姜丽冬等: ""超声波辅助酶解法提取林蛙头多糖及其抗氧化性的研究"", 《中国食品科学技术学会第十二届年会暨第八届中美食品业高层论坛论文摘要集》 * |
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Application publication date: 20170815 |