CN107001451A - 检测组织样品中裂解的snap25的方法 - Google Patents

检测组织样品中裂解的snap25的方法 Download PDF

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Publication number
CN107001451A
CN107001451A CN201580042335.2A CN201580042335A CN107001451A CN 107001451 A CN107001451 A CN 107001451A CN 201580042335 A CN201580042335 A CN 201580042335A CN 107001451 A CN107001451 A CN 107001451A
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CN
China
Prior art keywords
snap25
antibody
ser
bont
thr
Prior art date
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Pending
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CN201580042335.2A
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English (en)
Chinese (zh)
Inventor
R·S·布罗伊德
B·蔡
E·费尔南德斯-萨拉斯
J·弗朗西斯
C·罗姆
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Allergan Inc
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Allergan Inc
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Application filed by Allergan Inc filed Critical Allergan Inc
Priority claimed from PCT/US2015/039372 external-priority patent/WO2016007508A1/en
Publication of CN107001451A publication Critical patent/CN107001451A/zh
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/5695—Mycobacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/34—Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/567—Framework region [FR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/72—Increased effector function due to an Fc-modification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/952—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from bacteria

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201580042335.2A 2014-07-07 2015-07-07 检测组织样品中裂解的snap25的方法 Pending CN107001451A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201462021379P 2014-07-07 2014-07-07
US62/021,379 2014-07-07
US201562158900P 2015-05-08 2015-05-08
US62/158,900 2015-05-08
US201562163829P 2015-05-19 2015-05-19
US62/163,829 2015-05-19
PCT/US2015/039372 WO2016007508A1 (en) 2014-07-07 2015-07-07 Method of detecting cleaved snap25 in tissue samples

Publications (1)

Publication Number Publication Date
CN107001451A true CN107001451A (zh) 2017-08-01

Family

ID=55016834

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201580042335.2A Pending CN107001451A (zh) 2014-07-07 2015-07-07 检测组织样品中裂解的snap25的方法

Country Status (6)

Country Link
US (2) US10527620B2 (https=)
EP (2) EP3567055A1 (https=)
JP (1) JP6630717B2 (https=)
KR (1) KR20170026624A (https=)
CN (1) CN107001451A (https=)
CA (1) CA2954504A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114072673A (zh) * 2018-11-29 2022-02-18 秀杰股份公司 基于细胞的用于测定肉毒杆菌毒素活性的方法
CN119044508A (zh) * 2024-10-31 2024-11-29 兰州生物技术开发有限公司 检测肉毒毒素活性或突触体相关蛋白的试剂盒及检测方法
CN119044510A (zh) * 2024-10-31 2024-11-29 兰州生物技术开发有限公司 一种测定肉毒毒素活性或snap25的试剂盒、抗体及方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
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PT2271670E (pt) * 2008-03-14 2014-11-28 Allergan Inc Ensaio imunobaseado da atividade do serotipo a da toxina botulínica
KR101983216B1 (ko) * 2018-11-29 2019-05-29 주식회사 에이비바이오 보툴리눔 독소 활성을 결정하기 위한 항체, 및 이를 이용한 활성 측정방법
WO2022043924A1 (en) * 2020-08-28 2022-03-03 Galderma Holding SA Novel x-aptamers for the use in detection of snap25

Citations (4)

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US6331415B1 (en) * 1983-04-08 2001-12-18 Genentech, Inc. Methods of producing immunoglobulins, vectors and transformed host cells for use therein
CN102422158A (zh) * 2009-03-13 2012-04-18 阿勒根公司 基于免疫的重靶向内肽酶活性测定
CN102548549A (zh) * 2009-08-25 2012-07-04 细胞研究有限公司 利用外代谢转换剂(辅酶q10)治疗肉瘤的方法
CN105683212A (zh) * 2013-08-21 2016-06-15 香港理工大学 高催化活性的基因改造梭菌属神经毒素

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WO2003011902A1 (en) * 2001-07-27 2003-02-13 Kenton S.R.L. Identification of specific tumour antigens by selection of cdna libraries with sera
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ES2374822T3 (es) 2004-02-24 2012-02-22 Allergan, Inc. Ensayo de selección de toxinas botul�?nicas.
US7399607B2 (en) 2004-09-22 2008-07-15 Allergan, Inc. Fluorescence polarization assays for determining clostridial toxin activity
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EP2293064B1 (en) 2005-04-05 2012-04-04 Allergan, Inc. Lipophilic dye-based FRET assays for clostridial toxin activity
US20100227868A1 (en) 2007-09-20 2010-09-09 Johnson Brent A Treatment methods with brimonidine
KR101604515B1 (ko) * 2008-03-14 2016-03-17 알러간, 인코포레이티드 면역-기반 보툴리눔 독소 세로타입 ａ 활성 검정
PT2271670E (pt) 2008-03-14 2014-11-28 Allergan Inc Ensaio imunobaseado da atividade do serotipo a da toxina botulínica
BRPI1008940A2 (pt) 2009-03-13 2020-10-27 Allergan, Inc. linhagem de célula clonal estabelecida suscetível à intoxicação por bont/a

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US6331415B1 (en) * 1983-04-08 2001-12-18 Genentech, Inc. Methods of producing immunoglobulins, vectors and transformed host cells for use therein
CN102422158A (zh) * 2009-03-13 2012-04-18 阿勒根公司 基于免疫的重靶向内肽酶活性测定
CN102548549A (zh) * 2009-08-25 2012-07-04 细胞研究有限公司 利用外代谢转换剂(辅酶q10)治疗肉瘤的方法
CN105683212A (zh) * 2013-08-21 2016-06-15 香港理工大学 高催化活性的基因改造梭菌属神经毒素

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114072673A (zh) * 2018-11-29 2022-02-18 秀杰股份公司 基于细胞的用于测定肉毒杆菌毒素活性的方法
CN114072673B (zh) * 2018-11-29 2024-07-26 秀杰股份公司 基于细胞的用于测定肉毒杆菌毒素活性的方法
CN119044508A (zh) * 2024-10-31 2024-11-29 兰州生物技术开发有限公司 检测肉毒毒素活性或突触体相关蛋白的试剂盒及检测方法
CN119044510A (zh) * 2024-10-31 2024-11-29 兰州生物技术开发有限公司 一种测定肉毒毒素活性或snap25的试剂盒、抗体及方法
CN119044508B (zh) * 2024-10-31 2025-01-28 兰州生物技术开发有限公司 检测肉毒毒素活性或突触体相关蛋白的试剂盒及检测方法
CN119044510B (zh) * 2024-10-31 2025-01-28 兰州生物技术开发有限公司 一种测定肉毒毒素活性或snap25的试剂盒、抗体及方法

Also Published As

Publication number Publication date
US20160003824A1 (en) 2016-01-07
EP3166971A1 (en) 2017-05-17
JP2017524689A (ja) 2017-08-31
KR20170026624A (ko) 2017-03-08
US10527620B2 (en) 2020-01-07
CA2954504A1 (en) 2016-01-14
JP6630717B2 (ja) 2020-01-15
EP3166971B1 (en) 2019-09-11
US20190317095A1 (en) 2019-10-17
EP3567055A1 (en) 2019-11-13

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