CN106961874A - A kind of method for promoting twigs of the chaste tree seed to sprout - Google Patents
A kind of method for promoting twigs of the chaste tree seed to sprout Download PDFInfo
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- CN106961874A CN106961874A CN201710226005.XA CN201710226005A CN106961874A CN 106961874 A CN106961874 A CN 106961874A CN 201710226005 A CN201710226005 A CN 201710226005A CN 106961874 A CN106961874 A CN 106961874A
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- twigs
- chaste tree
- tree seed
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- blade
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The invention discloses a kind of method for promoting twigs of the chaste tree seed to sprout, including：(1) single treatment;（2）After-treatment：(3) three processing；(4) vernalization.By the processing of each step of the invention, obvious facilitation Verbenaceae germination ratio is shown as to the sprouting of twigs of the chaste tree seed and germination index is respectively provided with significant raising.
Description
Technical field
The invention belongs to twigs of the chaste tree seed breeding technique field, and in particular to a kind of method that promotion twigs of the chaste tree seed is sprouted.
Background technology
The twigs of the chaste tree are the excellent shrub species of northern China arid area water and soil conservation.It is without artificial seeding, and plant just can shape
Into natural phytobiocoenose, barren land is set to put on green clothing.Twigs of the chaste tree well developed root system, it is as fine as a spider's web, frame up in very thin soil layer,
Can not only absorb water, retaining, and can also secret out of a kind of plant acid, soil is resolved into firm rock corrosion.Its that cyclopentadienyl
Close branch and dry branches and fallen leaves, as sponge is laid down on soil, can water conservation, conserve water and soil, but can culture fertility,
Extremely important effect is played in planting trees on barren hills and environmental protection, and the twigs of the chaste tree have good toughness and intensity, are wicker plaiting articles
Good raw material.
With being continuously increased for wicker products demand, the demand of twigs of the chaste tree raw material is also continuously increased, the wild twigs of the chaste tree because
Streaking is even, yield is relatively low, and the demand of industry can not be met, therefore many people start the artificial growth twigs of the chaste tree.Existing chaste tree
During bar seed seedling-raising, seed germination rate is low, and the seedling quality of incubation is poor, and the yield of the twigs of the chaste tree is relatively low.
The content of the invention
The purpose of the present invention is that there is provided a kind of method for promoting twigs of the chaste tree seed to sprout the problem of being directed to existing.
The present invention is achieved by the following technical solutions：
A kind of method for promoting twigs of the chaste tree seed to sprout, comprises the following steps：
(1) single treatment：Twigs of the chaste tree seed is put into soak a and soaked 1.2 hours, soaking temperature is 30 DEG C, is then taken out,
30s is soaked using clear water, further takes out, twigs of the chaste tree seed is layered on the feeding plate of plasma processor, in plasma processor
Make plasma treatment with nitrogen, the flow of nitrogen is 0.40-0.45L/min, and power is 30-40W, and processing time is 20-25s；
The soak a preparation methods are：Opal blade of grass, ant grass blade are pressed 1：After the mixing of 3 mass ratios, ant grass blade quality is added
8 times of clear water, are heated to after boiling, are incubated 30min, and then filtering removes filter residue, obtains filtrate, as soak a；
(2) after-treatment：The twigs of the chaste tree seed of above-mentioned processing is put into soak b and soaked 1.8 hours, soaking temperature is 32 DEG C,
Then take out, 30s is soaked using clear water, further takes out, twigs of the chaste tree seed is placed in single treatment 3-5min in corona field, a corona
Field field strength is 230KV/m, and twigs of the chaste tree seed then is placed in into progress after-treatment 30-50s, secondary corona field field strength in corona field again
For 260KV/m；The soak b preparation methods are：Tang blade of grass, caput grass blade are pressed 5：After the mixing of 2 mass ratios, snake is added
5 times of clear water of tongue blade of grass quality, after being beaten, the ethanol of 2 times of addition HERBA HEDYOTIS DIFFUSAE leaf quality is small with the stirring 3 of 220r/min rotating speeds
Shi Hou, carries out separation of solid and liquid under high speed centrifugation, and gained liquid is soak b；
(3) three processing：The twigs of the chaste tree seed of above-mentioned processing is placed into during relative air humidity is the environment that 70%, temperature is 32 DEG C
Place 1 hour, while carrying out uniform irradiation using the white light of 500-600lux intensities of illumination, then take out, twigs of the chaste tree seed is spread
On the feeding plate of plasma processor, make plasma treatment with argon gas in plasma processor, the flow of nitrogen is 0.45-
0.50L/min, power is 50-60W, and processing time is 15-18s；（4）Vernalization：Vernalization, 80% twigs of the chaste tree seed are carried out at 30 DEG C
After showing money or valuables one carries unintentionally, you can sowed.
Further, the plasma processor uses PSA2000, and the frequency of the exciting electrode of plasma gas is
13.56Mpa, electrode spacing is 1cm.
Further, the ultracentrifugal speed is 2500r/min.
Further, the twigs of the chaste tree seed is placed in when being handled in corona field, and environment temperature is 32 DEG C, relative air humidity is
65%。
The present invention has advantages below compared with prior art：Twigs of the chaste tree seed has light sleep characteristic, and conventional treatment is to chaste tree
Bar seed dormancy breaks limitation, causes Verbenaceae germination ratio relatively low, and the present invention by carrying out respectively to twigs of the chaste tree seed
The dormancy of twigs of the chaste tree seed is broken in three processing, is improved the germination percentage of twigs of the chaste tree seed, is passed through the soak a immersions in single treatment
Processing and the synergy of plasma treatment, enable to twigs of the chaste tree seed endoperoxides hydrogenase activity to significantly improve, and peroxidating
The height of hydrogenase activity, into positive correlation, is caused raw in twigs of the chaste tree seed with the vigor of seed by improving the activity of catalase
Reason metabolism is more vigorous, so as to breaking dormancy, can be caused in twigs of the chaste tree seed by after-treatment, Acid Phosphatase Activity
It is greatly improved with amount of ATP, Acid Phosphatase Activity gradually increases with the sprouting process of seed, its
The height of activity shows the speed of seed sprouting, by improving Acid Phosphatase Activity, to accelerate the sprouting of twigs of the chaste tree seed,
The sprouting of twigs of the chaste tree seed is made finally by three processing to promote consolidation processing, can further promote twigs of the chaste tree seed vitality, it is right
The sprouting of twigs of the chaste tree seed shows as obvious facilitation Verbenaceae germination ratio and germination index is respectively provided with significant raising.
Embodiment
Embodiment 1
A kind of method for promoting twigs of the chaste tree seed to sprout, comprises the following steps：
(1) single treatment：Twigs of the chaste tree seed is put into soak a and soaked 1.2 hours, soaking temperature is 30 DEG C, is then taken out,
30s is soaked using clear water, further takes out, twigs of the chaste tree seed is layered on the feeding plate of plasma processor, in plasma processor
Make plasma treatment with nitrogen, the flow of nitrogen is 0.40L/min, and power is 30W, and processing time is 20s；The soak a
Preparation method is：Opal blade of grass, ant grass blade are pressed 1：After the mixing of 3 mass ratios, ant grass blade 8 times of clear water of quality are added, plus
Heat is incubated 30min to after seething with excitement, and then filtering removes filter residue, obtains filtrate, as soak a；
(2) after-treatment：The twigs of the chaste tree seed of above-mentioned processing is put into soak b and soaked 1.8 hours, soaking temperature is 32 DEG C,
Then take out, 30s is soaked using clear water, further takes out, twigs of the chaste tree seed is placed in single treatment 3min in corona field, a corona field
Field strength is 230KV/m, and twigs of the chaste tree seed then is placed in into progress after-treatment 30s in corona field again, and secondary corona field field strength is
260KV/m；The soak b preparation methods are：Tang blade of grass, caput grass blade are pressed 5：After the mixing of 2 mass ratios, tang is added
5 times of clear water of blade of grass quality, after being beaten, the ethanol of 2 times of addition HERBA HEDYOTIS DIFFUSAE leaf quality is stirred 3 hours with 220r/min rotating speeds
Afterwards, separation of solid and liquid is carried out under high speed centrifugation, gained liquid is soak b；
(3) three processing：The twigs of the chaste tree seed of above-mentioned processing is placed into during relative air humidity is the environment that 70%, temperature is 32 DEG C
Place 1 hour, while carrying out uniform irradiation using the white light of 500lux intensities of illumination, then take out, twigs of the chaste tree seed is layered on
On the feeding plate of ion processing device, make plasma treatment with argon gas in plasma processor, the flow of nitrogen is 0.45L/
Min, power is 50W, and processing time is 15s；
（4）Vernalization：Vernalization is carried out at 30 DEG C, after 80% twigs of the chaste tree seed shows money or valuables one carries unintentionally, you can sowed.
The plasma processor uses PSA2000, and the frequency of the exciting electrode of plasma gas is 13.56Mpa, electrode
Away from for 1cm.
The ultracentrifugal speed is 2500r/min.
The twigs of the chaste tree seed is placed in when being handled in corona field, and environment temperature is 32 DEG C, relative air humidity is 65%.
Embodiment 2
A kind of method for promoting twigs of the chaste tree seed to sprout, comprises the following steps：
(1) single treatment：Twigs of the chaste tree seed is put into soak a and soaked 1.2 hours, soaking temperature is 30 DEG C, is then taken out,
30s is soaked using clear water, further takes out, twigs of the chaste tree seed is layered on the feeding plate of plasma processor, in plasma processor
Make plasma treatment with nitrogen, the flow of nitrogen is 0.45L/min, and power is 40W, and processing time is 25s；The soak a
Preparation method is：Opal blade of grass, ant grass blade are pressed 1：After the mixing of 3 mass ratios, ant grass blade 8 times of clear water of quality are added, plus
Heat is incubated 30min to after seething with excitement, and then filtering removes filter residue, obtains filtrate, as soak a；
(2) after-treatment：The twigs of the chaste tree seed of above-mentioned processing is put into soak b and soaked 1.8 hours, soaking temperature is 32 DEG C,
Then take out, 30s is soaked using clear water, further takes out, twigs of the chaste tree seed is placed in single treatment 5min in corona field, a corona field
Field strength is 230KV/m, and twigs of the chaste tree seed then is placed in into progress after-treatment 50s in corona field again, and secondary corona field field strength is
260KV/m；The soak b preparation methods are：Tang blade of grass, caput grass blade are pressed 5：After the mixing of 2 mass ratios, tang is added
5 times of clear water of blade of grass quality, after being beaten, the ethanol of 2 times of addition HERBA HEDYOTIS DIFFUSAE leaf quality is stirred 3 hours with 220r/min rotating speeds
Afterwards, separation of solid and liquid is carried out under high speed centrifugation, gained liquid is soak b；
(3) three processing：The twigs of the chaste tree seed of above-mentioned processing is placed into during relative air humidity is the environment that 70%, temperature is 32 DEG C
Place 1 hour, while carrying out uniform irradiation using the white light of 600lux intensities of illumination, then take out, twigs of the chaste tree seed is layered on
On the feeding plate of ion processing device, make plasma treatment with argon gas in plasma processor, the flow of nitrogen is 0.50L/
Min, power is 60W, and processing time is 18s；
（4）Vernalization：Vernalization is carried out at 30 DEG C, after 80% twigs of the chaste tree seed shows money or valuables one carries unintentionally, you can sowed.
The plasma processor uses PSA2000, and the frequency of the exciting electrode of plasma gas is 13.56Mpa, electrode
Away from for 1cm.
The ultracentrifugal speed is 2500r/min.
The twigs of the chaste tree seed is placed in when being handled in corona field, and environment temperature is 32 DEG C, relative air humidity is 65%.
Embodiment 3
A kind of method for promoting twigs of the chaste tree seed to sprout, it is characterised in that comprise the following steps：
(1) single treatment：Twigs of the chaste tree seed is put into soak a and soaked 1.2 hours, soaking temperature is 30 DEG C, is then taken out,
30s is soaked using clear water, further takes out, twigs of the chaste tree seed is layered on the feeding plate of plasma processor, in plasma processor
Make plasma treatment with nitrogen, the flow of nitrogen is 0.42L/min, and power is 32W, and processing time is 245s；The soak a
Preparation method is：Opal blade of grass, ant grass blade are pressed 1：After the mixing of 3 mass ratios, ant grass blade 8 times of clear water of quality are added, plus
Heat is incubated 30min to after seething with excitement, and then filtering removes filter residue, obtains filtrate, as soak a；
(2) after-treatment：The twigs of the chaste tree seed of above-mentioned processing is put into soak b and soaked 1.8 hours, soaking temperature is 32 DEG C,
Then take out, 30s is soaked using clear water, further takes out, twigs of the chaste tree seed is placed in single treatment 4min in corona field, a corona field
Field strength is 230KV/m, and twigs of the chaste tree seed then is placed in into progress after-treatment 40s in corona field again, and secondary corona field field strength is
260KV/m；The soak b preparation methods are：Tang blade of grass, caput grass blade are pressed 5：After the mixing of 2 mass ratios, tang is added
5 times of clear water of blade of grass quality, after being beaten, the ethanol of 2 times of addition HERBA HEDYOTIS DIFFUSAE leaf quality is stirred 3 hours with 220r/min rotating speeds
Afterwards, separation of solid and liquid is carried out under high speed centrifugation, gained liquid is soak b；
(3) three processing：The twigs of the chaste tree seed of above-mentioned processing is placed into during relative air humidity is the environment that 70%, temperature is 32 DEG C
Place 1 hour, while carrying out uniform irradiation using the white light of 520lux intensities of illumination, then take out, twigs of the chaste tree seed is layered on
On the feeding plate of ion processing device, make plasma treatment with argon gas in plasma processor, the flow of nitrogen is 0.46L/
Min, power is 52W, and processing time is 17s；
（4）Vernalization：Vernalization is carried out at 30 DEG C, after 80% twigs of the chaste tree seed shows money or valuables one carries unintentionally, you can sowed.
The plasma processor uses PSA2000, and the frequency of the exciting electrode of plasma gas is 13.56Mpa, electrode
Away from for 1cm.
The ultracentrifugal speed is 2500r/min.
The twigs of the chaste tree seed is placed in when being handled in corona field, and environment temperature is 32 DEG C, relative air humidity is 65%.
Comparative example 1：Differed only in embodiment 1 without step（1）Processing.
Comparative example 2：Step is differed only in embodiment 1（2）In without secondary corona field handle.
Comparative example 3：It is step with the difference of embodiment 1（3）In do not use white light photo-irradiation treatment.
Comparative example 4：It is step with the difference of embodiment 1（3）In do not use plasma device to be handled.
Experiment：It is 57% to test twigs of the chaste tree physical purity of seed, and water content is 6.5%, and dry granular weight is 8.6g, and seed superiority is 66%, is divided into
7 groups, every group 20, embodiment is respectively adopted and is handled with comparative example method；（Embodiment is averaged）
Germination test：It is placed in ZF-32 type illumination germinators and the type electro-heating standing-temperature cultivators of HHB II, determines and just sprout day, average hair
The Desiccation tolerance index such as bud time, germination index and germination percentage, just sprouts and refers to day first day that radicle exposes, the time exposed
Early explanation seed vitality is high, and average germinating time refers to the number of days that germination in first day is germinateed to last day, the short theory of number of days of germinateing
Bright seed vitality is high, germination index：GI=∑（Gt/Dt）, wherein, GI is germination index, and Gt is germination grain number, and Dt is corresponding
Germinate number of days：
Table 1
As can be seen from Table 1, the inventive method processing twigs of the chaste tree seed, can significantly improve the germination percentage of twigs of the chaste tree seed, shorten chaste tree
The germinating time of bar seed.
Claims (4)
1. a kind of method for promoting twigs of the chaste tree seed to sprout, it is characterised in that comprise the following steps：
(1) single treatment：Twigs of the chaste tree seed is put into soak a and soaked 1.2 hours, soaking temperature is 30 DEG C, is then taken out,
30s is soaked using clear water, further takes out, twigs of the chaste tree seed is layered on the feeding plate of plasma processor, in plasma processor
Make plasma treatment with nitrogen, the flow of nitrogen is 0.40-0.45L/min, and power is 30-40W, and processing time is 20-25s；
The soak a preparation methods are：Opal blade of grass, ant grass blade are pressed 1：After the mixing of 3 mass ratios, ant grass blade quality is added
8 times of clear water, are heated to after boiling, are incubated 30min, and then filtering removes filter residue, obtains filtrate, as soak a；
(2) after-treatment：The twigs of the chaste tree seed of above-mentioned processing is put into soak b and soaked 1.8 hours, soaking temperature is 32 DEG C,
Then take out, 30s is soaked using clear water, further takes out, twigs of the chaste tree seed is placed in single treatment 3-5min in corona field, a corona
Field field strength is 230KV/m, and twigs of the chaste tree seed then is placed in into progress after-treatment 30-50s, secondary corona field field strength in corona field again
For 260KV/m；The soak b preparation methods are：Tang blade of grass, caput grass blade are pressed 5：After the mixing of 2 mass ratios, snake is added
5 times of clear water of tongue blade of grass quality, after being beaten, the ethanol of 2 times of addition HERBA HEDYOTIS DIFFUSAE leaf quality is small with the stirring 3 of 220r/min rotating speeds
Shi Hou, carries out separation of solid and liquid under high speed centrifugation, and gained liquid is soak b；
(3) three processing：The twigs of the chaste tree seed of above-mentioned processing is placed into during relative air humidity is the environment that 70%, temperature is 32 DEG C
Place 1 hour, while carrying out uniform irradiation using the white light of 500-600lux intensities of illumination, then take out, twigs of the chaste tree seed is spread
On the feeding plate of plasma processor, make plasma treatment with argon gas in plasma processor, the flow of nitrogen is 0.45-
0.50L/min, power is 50-60W, and processing time is 15-18s；（4）Vernalization：Vernalization, 80% twigs of the chaste tree seed are carried out at 30 DEG C
After showing money or valuables one carries unintentionally, you can sowed.
2. a kind of method for promoting twigs of the chaste tree seed to sprout as claimed in claim 1, it is characterised in that the plasma processor
Using PSA2000, the frequency of the exciting electrode of plasma gas is 13.56Mpa, and electrode spacing is 1cm.
3. a kind of method for promoting twigs of the chaste tree seed to sprout as claimed in claim 1, it is characterised in that the ultracentrifugal speed
Spend for 2500r/min.
4. a kind of method for promoting twigs of the chaste tree seed to sprout as claimed in claim 1, it is characterised in that the twigs of the chaste tree seed is placed in
When being handled in corona field, environment temperature is 32 DEG C, relative air humidity is 65%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710226005.XA CN106961874A (en) | 2017-04-08 | 2017-04-08 | A kind of method for promoting twigs of the chaste tree seed to sprout |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710226005.XA CN106961874A (en) | 2017-04-08 | 2017-04-08 | A kind of method for promoting twigs of the chaste tree seed to sprout |
Publications (1)
| Publication Number | Publication Date |
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| CN106961874A true CN106961874A (en) | 2017-07-21 |
Family
ID=59337195
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201710226005.XA Withdrawn CN106961874A (en) | 2017-04-08 | 2017-04-08 | A kind of method for promoting twigs of the chaste tree seed to sprout |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1031168A (en) * | 1987-08-14 | 1989-02-22 | 鞍山市静电技术研究所 | Apparatus for treating seeds by electrostatic field |
| CN101336573A (en) * | 2008-08-11 | 2009-01-07 | 长江大学 | Method for increasing Verbenaceae germination ratio |
| CN103650699A (en) * | 2013-12-11 | 2014-03-26 | 苏州市奥普斯等离子体科技有限公司 | Plasma treatment device for seeds and treatment method of plasma treatment device |
| CN104956808A (en) * | 2015-06-10 | 2015-10-07 | 马魁 | Vitex negundo L. seed processing method |
| CN106134713A (en) * | 2016-06-30 | 2016-11-23 | 安徽金培因科技有限公司 | A kind of Caulis et Folium Lactucae Sativae implantation methods |
-
2017
- 2017-04-08 CN CN201710226005.XA patent/CN106961874A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1031168A (en) * | 1987-08-14 | 1989-02-22 | 鞍山市静电技术研究所 | Apparatus for treating seeds by electrostatic field |
| CN101336573A (en) * | 2008-08-11 | 2009-01-07 | 长江大学 | Method for increasing Verbenaceae germination ratio |
| CN103650699A (en) * | 2013-12-11 | 2014-03-26 | 苏州市奥普斯等离子体科技有限公司 | Plasma treatment device for seeds and treatment method of plasma treatment device |
| CN104956808A (en) * | 2015-06-10 | 2015-10-07 | 马魁 | Vitex negundo L. seed processing method |
| CN106134713A (en) * | 2016-06-30 | 2016-11-23 | 安徽金培因科技有限公司 | A kind of Caulis et Folium Lactucae Sativae implantation methods |
Non-Patent Citations (1)
| Title |
|---|
| "《基础物理学》" * |
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Application publication date: 20170721 |
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