CN106922750A - A kind of fermentation of Chinese herbal medicine improver of water quality for improving mud blood clam seed rearing and its preparation application method - Google Patents
A kind of fermentation of Chinese herbal medicine improver of water quality for improving mud blood clam seed rearing and its preparation application method Download PDFInfo
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- CN106922750A CN106922750A CN201710085152.XA CN201710085152A CN106922750A CN 106922750 A CN106922750 A CN 106922750A CN 201710085152 A CN201710085152 A CN 201710085152A CN 106922750 A CN106922750 A CN 106922750A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
Abstract
The invention discloses a kind of fermentation of Chinese herbal medicine improver of water quality for improving mud blood clam seed rearing, it is characterised in that be made up of the raw material of the weight/mass percentage composition of following each component：Gynostemma pentaphylla 5~10%, polygala 2~5%, Semen Cuscutae 4~8%, rough gentian 3 ~ 8%, golden cypress 5 ~ 10%, the bark of official magnolia 5 ~ 10%, purslane 5 ~ 15%, dandelion 4 ~ 8%, corydlis bungeana 6 ~ 12%, Radix Isatidis 10 ~ 15%, cordate houttuynia 5 ~ 10%, Snakegourd Fruit 5 ~ 10% and rhodiola root 4 ~ 8%.The invention also discloses the fermentation of Chinese herbal medicine improver of water quality preparation and application for improving mud blood clam seed rearing.Herbal raw material source of the present invention is wide, it is cheap, production and processing technology is simple, is soaked using ultrasonic assistant, then carry out aerobic fermentation and anaerobic fermentation, improve Chinese herbal medicine functional components and beneficial microbe composition, with improvement mud blood clam seedling water, effectively suppress the outburst of disease microorganism, strengthen mud blood clam seed young immune disease-resistance ability, the survival rate of seed is improved, is a kind of preferable, green, environmental protection, safety, new improver of water quality.
Description
Technical field
The present invention relates to a kind of marine shellfish Chinese herbal medicine improver of water quality, mud blood clam seed rearing is improved more particularly, to one kind
Fermentation of Chinese herbal medicine improver of water quality and its prepare application method.
Background technology
Mud blood clam, belongs to Mollusca, and Bivalvia, Taxodonta, blood clam section, blood clam belongs to, and is the cultivated shellfish of Chinese tradition, coastal each
Ground is distributed.Hebei, Shandong, Zhejiang, Fujian, Guangdong are propagated artificially, and yield is rich.Mud blood clam meat flavour is delicious, can be fresh
Food or wine stain, also can be made into dry product, and blood clam meat contains a large amount of protein and vitamin, and blood clam blood is scarlet, and the edge of meat has as a spun gold
Colo(u)r streak.Shell can be used as medicine, and have functions that disappear clot and resolving sputum product.Consumption demand amount constantly expands, and promotes the fast of mud blood clam aquaculture
Speed development, the increase to seed demand, artificial breeding is also developed rapidly.However, in mud blood clam nursery production, often sending out
Raw two peak mortalities：One is that, in the straight hinge larvas phase, two are occurred in period of metamorphosis to juvenile mollusk radial rib（About 300 μm big
It is small）.Analysis main cause of death has：1. harmful microorganism causes disease to infect；2. the environment discomfort such as water quality deterioration causes.In the past
In mud blood clam seedling raising process, pathogen growth is suppressed by adding antibiotic in seedling water, improve the survival rate of seed.
However, antibiotic is easily caused disease microorganism developing immunity to drugs, antibiotic is caused to fail；It is easily caused using antibiotic in addition
Larval metamorphosis deformity and mud blood clam medicament residue, the mud blood clam speed of growth is unhappy in causing later stage breeding process, and yield is not high, serious shadow
Ring the cultivation income of raiser；Furthermore water quality improvement is have ignored in seedling raising process, causes can not inherently to improve seed surviving
Rate.
From the point of view of existing patent with paper, relevant improvement seedling water water quality is had no, control mud blood clam seed disease, improved
The report of the fermentation of Chinese herbal medicine improver of water quality of mud blood clam seed yield, Chinese herb feed additive have medicine source extensively, efficiently, the resistance to the action of a drug
Not significantly, the advantages of toxic and side effect is small, during aquatic animal disease is prevented and treated, in addition to having the property of medicine and trophism concurrently, also have
Effect of aquatic livestock production performance and efficiency of feed utilization is improved, so be increasingly taken seriously, using also increasingly extensive.It is micro-
Biofermentation Chinese medicine additive is the reform and innovation to traditional Chinese medicine process technology, with various features and advantage.By this skill
Art introduces Chinese herbal medicine improver of water quality, can significantly improve its main active substances polysaccharide and active beneficial microorganism content,
Scientific and technological content is substantially improved, and effect is more significantly, and quality is more stablized, and benefit is more obvious, can significantly improve immunity of organism
Power, strengthens the level of aquatic livestock resist the disease, improves nursery water quality, for aquatic livestock in the current culture fishery of solution
Various problems have important application value.
The content of the invention
It is an object of the invention to the above mentioned problem according to present in existing mud blood clam seedling-raising technique, exploitation is a kind of to improve mud blood clam
The fermentation of Chinese herbal medicine improver of water quality of seed rearing come strengthen mud blood clam seed immunity of organism, improve mud blood clam seedling water water quality
Environment, reduce aquaculture pollution that metabolin discharge causes, reduce mud blood clam seed disease and occur, reduce mud blood clam seed abnormal rate,
The survival rate and yield of mud blood clam seed are improved, mud blood clam aquaculture healthy and sustainable development is promoted with this.
Above-mentioned purpose of the present invention is to be achieved by the following technical programs：
A kind of fermentation of Chinese herbal medicine improver of water quality for improving mud blood clam seed rearing, it is characterised in that by the quality hundred of following each component
The raw material of point content is made：Gynostemma pentaphylla 5~10%, polygala 2~5%, Semen Cuscutae 4~8%, the % of rough gentian 3 ~ 8, golden cypress 5 ~ 10%, the bark of official magnolia 5
~ 10%, purslane 5 ~ 15%, dandelion 4 ~ 8%, corydlis bungeana 6 ~ 12%, Radix Isatidis 10 ~ 15%, cordate houttuynia 5 ~ 10%, the He of Snakegourd Fruit 5 ~ 10%
Rhodiola root 4 ~ 8%.The percentage by weight sum of above-mentioned each component is 100%.
The present invention also provides preparation and the user of a kind of fermentation of Chinese herbal medicine improver of water quality for improving mud blood clam seed rearing
Method, specifically includes following steps：
（1）Get the raw materials ready：Impurity in each traditional Chinese medicinal material raw materials is rejected, it is clear with pure water according to above-mentioned each raw material of formula precise
Wash traditional Chinese medicinal material raw materials 3 ~ 4 times, be chopped into 5 ~ 8 mm, be well mixed, compound Chinese herb raw material is obtained；
（2）Extraction：To the pure water that 20 ~ 30 times are added in compound Chinese herb raw material, in the effect of 300 ~ 600 W ultrasonic assistants
Under, 95~100 DEG C of extraction temperature extracts 25~45 min.
（3）It is prepared by zymotic fluid：To being added in the Chinese herbal medicine feed liquid after extraction, 2.0~4.0% sucrose, 0.5~2.0% soybean are dense
Pix protein, 0.5~1.5% peptone, 0.3~0.5% K2HPO4, 0.1~0.4% MgSO4.7H2O, 0.01~0.04% CaCl2
With 0.01~0.03 % FeSO4.7H2O, pH are adjusted to 7.0~7.5.
（4）Aerobic fermentation：From liquid fermentation tank, liquid amount is 50~70%, 1~3% bacillus subtilis of inoculation, 1~
3% bacillus licheniformis and 2~4% S. cervisiaes, control 28~37 DEG C of fermentation temperature, the rpm of rotating speed 150~300, throughput
30~50 L/min, the h of fermentation time 24~36.
（5）Anaerobic fermentation：After aerobic fermentation terminates, 2~4% lactobacillus acidophilus of inoculation, 1~2% Bifidobacterium and 1~3% nitre
Change bacterium, 28~37 DEG C of fermentation temperature, the rpm of rotating speed 150~300, the h of temperature control anaerobic fermentation 12~24.
（6）Fermentation post processing：After the completion of fermentation, cross 100 ~ 150 mesh filter clothes and obtain ferment filtrate, obtain final product fermentation of Chinese herbal medicine water quality
Modifying agent.
（7）Usage and dosage：After mud blood clam seedling water changed a water every 2 days, addition seedling water volume 0.1 ~ 0.4%
Fermentation of Chinese herbal medicine improver of water quality.
The present invention provides a kind of fermentation of Chinese herbal medicine improver of water quality for improving mud blood clam seed rearing, with improvement mud blood clam nursery
Water body, effectively suppresses pathogenic microorganism outburst, strengthens mud blood clam seed immunity and premunition, improves mud blood clam seed larval growth
Can, the multiple efficacies such as the survival rate and yield of mud blood clam seed are improved, it is preferable, green, environmentally friendly, the peace of substitute antibiotics medicine
Entirely, new improver of water quality.Product rear pollution-free, noresidue, without any side effects, safety and environmental protection.Advantage of the invention exists
In：One is that the present invention is using ultrasonic assistant leaching, then carries out aerobic fermentation and anaerobic compared with the simple crushing of Chinese herbal medicine is added
Fermentation, improves Chinese herbal medicine functional components and beneficial microbe composition, can more effectively suppress disease microorganism, improves mud blood clam seed
Young immune disease-resistance ability and survival rate；Two is that raw material sources are wide in the present invention, and cheap, production and processing technology is simple.
Specific embodiment
Below by specific embodiment, the present invention will be further described.In the present invention, if not refering in particular to, Suo Youbai
Point than being unit of weight, all devices and raw material are commercially available or the industry is conventional, in following embodiments
Method, unless otherwise instructed, is this area conventional method.
Embodiment 1
It is a kind of improve mud blood clam seed rearing fermentation of Chinese herbal medicine improver of water quality, by herbal raw material gynostemma pentaphylla, polygala, Tu
Son, rough gentian, golden cypress, the bark of official magnolia, purslane, dandelion, corydlis bungeana, Radix Isatidis, cordate houttuynia, Snakegourd Fruit and rhodiola root composition.Chinese herbal medicine
The specific proportioning of each component is as shown in table 1 in raw material.
It is a kind of improve mud blood clam seed rearing fermentation of Chinese herbal medicine improver of water quality preparation and application, specifically include with
Under step：
（1）Get the raw materials ready：Impurity in each traditional Chinese medicinal material raw materials is rejected, it is clear with pure water according to above-mentioned each raw material of formula precise
Wash traditional Chinese medicinal material raw materials 3 times, be chopped into 7 mm, be well mixed, compound Chinese herb raw material is obtained；
（2）Extraction：To the pure water that 27 times are added in compound Chinese herb raw material, under the effect of 450 W ultrasonic assistants, extraction temperature
97 DEG C of degree, extracts 35 min.
（3）It is prepared by zymotic fluid：To added in the Chinese herbal medicine feed liquid after extraction 2.0% sucrose, 1.5% FSPC,
0.5% peptone, 0.3% K2HPO4、0.2% MgSO4.7H2O、0.02% CaCl2With 0.01% FeSO4.7H2O, pH are adjusted to 7.0.
（4）Aerobic fermentation：From liquid fermentation tank, liquid amount is 60%, 2% bacillus subtilis of inoculation, 1% lichens gemma
Bacillus and 2.5% S. cervisiae, control 32 DEG C of fermentation temperature, the rpm of rotating speed 240, the L/min of throughput 45, fermentation time 30
h。
（5）Anaerobic fermentation：After aerobic fermentation terminates, 2.5% lactobacillus acidophilus of inoculation, 1% Bifidobacterium, 2% nitrobacteria,
30 DEG C of fermentation temperature, the rpm of rotating speed 220, the h of temperature control anaerobic fermentation 16.
（6）Fermentation post processing：After the completion of fermentation, cross 120 mesh filter clothes and obtain ferment filtrate, obtain final product fermentation of Chinese herbal medicine water quality improvement
Agent.
（7）Usage and dosage：After mud blood clam seedling water changed a water every 2 days, the hair of addition seedling water volume 0.1%
Ferment Chinese herbal medicine improver of water quality.
Embodiment 2
It is a kind of improve mud blood clam seed rearing fermentation of Chinese herbal medicine improver of water quality, by herbal raw material gynostemma pentaphylla, polygala, Tu
Son, rough gentian, golden cypress, the bark of official magnolia, purslane, dandelion, corydlis bungeana, Radix Isatidis, cordate houttuynia, Snakegourd Fruit and rhodiola root composition.Chinese herbal medicine
The specific proportioning of each component is as shown in table 1 in raw material.
It is a kind of improve mud blood clam seed rearing fermentation of Chinese herbal medicine improver of water quality preparation and application, specifically include with
Under step：
（1）Get the raw materials ready：Impurity in each traditional Chinese medicinal material raw materials is rejected, it is clear with pure water according to above-mentioned each raw material of formula precise
Wash traditional Chinese medicinal material raw materials 3 times, be chopped into 6 mm, be well mixed, compound Chinese herb raw material is obtained；
（2）Extraction：To the pure water that 30 times are added in compound Chinese herb raw material, under the effect of 500 W ultrasonic assistants, extraction temperature
95 DEG C of degree, extracts 40 min.
（3）It is prepared by zymotic fluid：To added in the Chinese herbal medicine feed liquid after extraction 2.5% sucrose, 1.0% FSPC,
1.0% peptone, 0.4% K2HPO4、0.2% MgSO4.7H2O、0.03% CaCl2With 0.02% FeSO4.7H2O, pH are adjusted to 7.2.
（4）Aerobic fermentation：From liquid fermentation tank, liquid amount is 55%, 1% bacillus subtilis of inoculation, 1.5% lichens bud
Spore bacillus and 3% S. cervisiae, control 35 DEG C of fermentation temperature, the rpm of rotating speed 180, the L/min of throughput 40, fermentation time 26
h。
（5）Anaerobic fermentation：After aerobic fermentation terminates, 2.5% lactobacillus acidophilus of inoculation, 1.5% Bifidobacterium and 1.5% nitrification
Bacterium, 32 DEG C of fermentation temperature, the rpm of rotating speed 240, the h of temperature control anaerobic fermentation 20.
（6）Fermentation post processing：After the completion of fermentation, cross 100 mesh filter clothes and obtain ferment filtrate, obtain final product fermentation of Chinese herbal medicine water quality improvement
Agent.
（7）Usage and dosage：After mud blood clam seedling water changed a water every 2 days, the hair of addition seedling water volume 0.2%
Ferment Chinese herbal medicine improver of water quality.
Embodiment 3
It is a kind of improve mud blood clam seed rearing fermentation of Chinese herbal medicine improver of water quality, by herbal raw material gynostemma pentaphylla, polygala, Tu
Son, rough gentian, golden cypress, the bark of official magnolia, purslane, dandelion, corydlis bungeana, Radix Isatidis, cordate houttuynia, Snakegourd Fruit and rhodiola root composition.Chinese herbal medicine
The specific proportioning of each component is as shown in table 1 in raw material.
It is a kind of improve mud blood clam seed rearing fermentation of Chinese herbal medicine improver of water quality preparation and application, specifically include with
Under step：
（1）Get the raw materials ready：Impurity in each traditional Chinese medicinal material raw materials is rejected, it is clear with pure water according to above-mentioned each raw material of formula precise
Wash traditional Chinese medicinal material raw materials 4 times, be chopped into 5 mm, be well mixed, compound Chinese herb raw material is obtained；
（2）Extraction：To the pure water that 25 times are added in compound Chinese herb raw material, under the effect of 550 W ultrasonic assistants, extraction temperature
98 DEG C of degree, extracts 30 min.
（3）It is prepared by zymotic fluid：To added in the Chinese herbal medicine feed liquid after extraction 3.0% sucrose, 0.5% FSPC,
1.5% peptone, 0.3% K2HPO4、0.3% MgSO4.7H2O、0.01% CaCl2With 0.03 % FeSO4.7H2O, pH are adjusted to 7.
5。
（4）Aerobic fermentation：From liquid fermentation tank, liquid amount is 67%, 1.5% bacillus subtilis of inoculation, 2% lichens bud
Spore bacillus and 3% S. cervisiae, control 30 DEG C of fermentation temperature, the rpm of rotating speed 260, the L/min of throughput 50, fermentation time 32
h。
（5）Anaerobic fermentation：After aerobic fermentation terminates, 2% lactobacillus acidophilus of inoculation, 1.5% Bifidobacterium and 1% nitrobacteria,
35 DEG C of fermentation temperature, the rpm of rotating speed 180, the h of temperature control anaerobic fermentation 22.
（6）Fermentation post processing：After the completion of fermentation, cross 150 mesh filter clothes and obtain ferment filtrate, obtain final product fermentation of Chinese herbal medicine water quality improvement
Agent.
（7）Usage and dosage：After mud blood clam seedling water changed a water every 2 days, the hair of addition seedling water volume 0.3%
Ferment Chinese herbal medicine improver of water quality.
The composition and ratio of each embodiment herbal raw material of table 1
| Component | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Gynostemma pentaphylla（%） | 9 | 6 | 8 |
| Polygala（%） | 2 | 4 | 3 |
| Semen Cuscutae（%） | 8 | 7 | 6 |
| Rough gentian（%） | 5 | 6 | 7 |
| Golden cypress（%） | 8 | 7 | 8 |
| The bark of official magnolia（%） | 8 | 8 | 10 |
| Purslane（%） | 11 | 9 | 10 |
| Dandelion（%） | 5 | 7 | 6 |
| Corydlis bungeana（%） | 10 | 9 | 6 |
| Radix Isatidis（%） | 15 | 13 | 11 |
| Cordate houttuynia（%） | 6 | 8 | 9 |
| Snakegourd Fruit（%） | 7 | 8 | 9 |
| Rhodiola root（%） | 6 | 8 | 7 |
| Summation（%） | 100 | 100 | 100 |
Seedling growth test
Take five 20 square metres, 0.5 meter of the depth of water, circular nursery pond（Respectively A groups, B groups, C groups, D groups, E groups）Carry out mud blood clam
Seed rearing is tested, larval rearing density：10/ml of great-hearted mud blood clam D-shaped larva；Bait：Early stage feeds chrysophyceae（5 ~ 100,000
Cell/ml）, the later stage feeds Chaetoceros（1 ~ 20,000 cells/ml）, flat algae（1.5 ~ 20,000 cells/ml）Deng；Inflation：Continuous charge, fills
Tolerance produces bubble to be advisable on a small quantity with the water surface；Condition of water quality：25 ~ 27 DEG C of water temperature, sea water specific gravity 1.015 ~ 1.017, pH 7.5 ~
8.0；Change water：Change water 1 time within every 2 days, each quantity of exchanged water 20 ~ 30% throws 0.5 ‰ EDTA, cultivation cycle 12 days after changing water.A、B、C
Group adds the Chinese herbal medicine improver of water quality in embodiment 1 ~ 3 respectively, and D groups are changed water and throw penicillin 0.3 ‰ every time, and E groups are without in
Herbal medicine and penicillin, as a comparison case, bait feeding mode is identical with feeding volume.
After cultivation 12 days, mud blood clam seed is determined, survival rate, abnormal rate, Average shell size, the knot for obtaining are counted respectively
Fruit is as shown in table 2.
The statistics of each embodiment of table 2 and comparative example
From table 2, it is apparent that mud blood clam weed survival rate and housing size are apparently higher than penicillin group and right in each embodiment
Mud blood clam weed survival rate and housing size in ratio, and in each embodiment mud blood clam seed abnormal rate rate significantly lower than penicillin group and
Mud blood clam seed abnormal rate in comparative example, illustrates that Chinese herb feed additive of the invention can remarkably promote the growth of mud blood clam seed, improve seedling
The survival rate planted, and mud blood clam seed abnormal rate can be significantly reduced.
Embodiment described above is a kind of preferably scheme of the invention, not makees any formal to the present invention
Limitation, also has other variants and remodeling on the premise of without departing from the technical scheme described in claim.
Claims (2)
1. it is a kind of improve mud blood clam seed rearing fermentation of Chinese herbal medicine improver of water quality, it is characterised in that by the matter of following each component
The raw material for measuring percentage composition is made：Gynostemma pentaphylla 5~10%, polygala 2~5%, Semen Cuscutae 4~8%, rough gentian 3 ~ 8%, golden cypress 5 ~ 10%, thickness
Piao 5 ~ 10%, purslane 5 ~ 15%, dandelion 4 ~ 8%, corydlis bungeana 6 ~ 12%, Radix Isatidis 10 ~ 15%, cordate houttuynia 5 ~ 10%, Snakegourd Fruit 5 ~ 10%
With rhodiola root 4 ~ 8%；The percentage by weight sum of each component is 100%.
2. it is a kind of it is as claimed in claim 1 improve mud blood clam seed rearing fermentation of Chinese herbal medicine improver of water quality preparation and use
Method, it is characterised in that comprise the following steps：
（1）Get the raw materials ready：Impurity in each traditional Chinese medicinal material raw materials is rejected, it is clear with pure water according to above-mentioned each raw material of formula precise
Wash traditional Chinese medicinal material raw materials 3 ~ 4 times, be chopped into 5 ~ 8 mm, be well mixed, compound Chinese herb raw material is obtained；
（2）Extraction：To the pure water that 20 ~ 30 times are added in compound Chinese herb raw material, in the effect of 300 ~ 600 W ultrasonic assistants
Under, 95~100 DEG C of extraction temperature extracts 25~45 min；
（3）It is prepared by zymotic fluid：To 2.0~4.0% sucrose of addition, 0.5~2.0% soybean concentrate egg in the Chinese herbal medicine feed liquid after extraction
In vain, 0.5~1.5% peptone, 0.3~0.5% K2HPO4, 0.1~0.4% MgSO4.7H2O, 0.01~0.04% CaCl2With
0.01~0.03 % FeSO4.7H2O, pH are adjusted to 7.0~7. 5；
（4）Aerobic fermentation：From liquid fermentation tank, liquid amount is 50~70%, 1~3% bacillus subtilis of inoculation, 1~3% ground
Clothing bacillus and 2~4% S. cervisiaes, 28~37 DEG C of fermentation temperature of control, the rpm of rotating speed 150~300, throughput 30~
50 L/min, the h of fermentation time 24~36；
（5）Anaerobic fermentation：After aerobic fermentation terminates, 2~4% lactobacillus acidophilus of inoculation, 1~2% Bifidobacterium, 1~3% nitrification are thin
Bacterium, 28~37 DEG C of fermentation temperature, the rpm of rotating speed 150~300, the h of temperature control anaerobic fermentation 12~24；
（6）Fermentation post processing：After the completion of fermentation, cross 100 ~ 150 mesh filter clothes and obtain ferment filtrate, obtain final product fermentation of Chinese herbal medicine water quality improvement
Agent；
（7）Usage and dosage：After mud blood clam seedling water changed a water every 2 days, the hair of addition seedling water volume 0.1 ~ 0.4%
Ferment Chinese herbal medicine improver of water quality.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108850011A (en) * | 2018-06-04 | 2018-11-23 | 林莹 | Fishpond fungicide |
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- 2017-02-17 CN CN201710085152.XA patent/CN106922750A/en active Pending
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| CN102246830A (en) * | 2011-03-28 | 2011-11-23 | 陈景河 | Prawn ecological water quality modifier |
| CN105174493A (en) * | 2015-10-10 | 2015-12-23 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of fermentation type Chinese herbal medicine water quality modifying agent for aquaculture and application thereof |
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| CN108850011A (en) * | 2018-06-04 | 2018-11-23 | 林莹 | Fishpond fungicide |
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