CN106883972B

CN106883972B - A herbal wine with kidney invigorating and yang supporting effects

Info

Publication number: CN106883972B
Application number: CN201710149968.4A
Authority: CN
Inventors: 陈美鑫
Original assignee: Guizhou Province Renhuan City Maotai Town Jingang Wine Industry Co ltd
Current assignee: Guizhou Province Renhuan City Maotai Town Jingang Wine Industry Co ltd
Priority date: 2017-03-14
Filing date: 2017-03-14
Publication date: 2020-09-04
Anticipated expiration: 2037-03-14
Also published as: CN106883972A

Abstract

The invention discloses a herbal wine with kidney tonifying and yang strengthening effects, which is prepared by processing a herbal composition, wherein the herbal composition comprises the following traditional Chinese medicine components in parts by weight: 4-8 parts of clove, 2-4 parts of cinnamon, 20-40 parts of Chinese yam, 8-15 parts of donkey-hide gelatin, 10-20 parts of wolfberry fruit, 15-20 parts of mulberry, 10-20 parts of fructus alpiniae oxyphyllae, 12-18 parts of rhizoma polygonati and 15-25 parts of raspberry. The invention has reasonable formula, obvious curative effect, small toxic and side effect and good health-care effect.

Description

A herbal wine with kidney invigorating and yang supporting effects

Technical Field

The invention relates to medicinal liquor, in particular to herbal wine with the functions of tonifying kidney and strengthening yang.

Background

The medicinal liquor has long source, is an important component of Chinese traditional medicine, is a treasure of cultural heritage, has a history of thousands of years from ancient times to present, and nowadays, along with the development of society and the improvement of living standard, the health consciousness of people is continuously enhanced, the medicinal liquor is more and more popular with people.

The traditional process of the medicinal liquor is to put the medicinal materials into the white spirit according to the mass ratio of the formula and soak the medicinal materials for a certain time, and then clear and transparent medicinal liquor is obtained by filtering and discarding residues. The quality of the wine depends on the content of effective components in the Chinese medicinal materials in the wine, and the content of the effective components is related to the content of alcohol in the white wine, the amount of the Chinese medicinal materials and the particle size, and is related to factors such as the soaking time, the room temperature and the like. Generally, the leaching of the active ingredients of the product produced by the traditional process soaking method is only about 10-20%, which not only affects the quality of the product, but also is a great waste for resources.

The yang-tonifying medicinal liquor is mainly used for patients with kidney-yang deficiency and erectile dysfunction and is mainly prepared from medlar, three penis and testis and penis and the like. The famous prescriptions include penis erection strengthening yang wine, epimedium wine, Qingsong age wine, lamb tonic wine, tortoise-age collection wine, ginseng and antler wine, fur seal kidney wine, etc. Although various yang-tonifying health-care wine sold in the market at present is available, most of the health-care wine has the problems of slow effect and unsatisfactory health-care effect due to unreasonable formula components.

Disclosure of Invention

The invention aims to provide the herbal wine with the functions of tonifying kidney and strengthening yang, which has the advantages of reasonable formula, obvious curative effect, small toxic and side effects and good health-care effect.

The technical scheme adopted by the invention for solving the technical problems is as follows:

a herbal wine with kidney-tonifying and yang-strengthening effects is prepared from a herbal composition, wherein the herbal composition comprises the following traditional Chinese medicine components in parts by weight: 4-8 parts of clove, 2-4 parts of cinnamon, 20-40 parts of Chinese yam, 8-15 parts of donkey-hide gelatin, 10-20 parts of wolfberry fruit, 15-20 parts of mulberry, 10-20 parts of fructus alpiniae oxyphyllae, 12-18 parts of rhizoma polygonati and 15-25 parts of raspberry.

Clove: warm middle energizer, warm kidney, descend adverse qi. It is indicated for hiccup, vomiting, regurgitation, dysentery, psychroalgia, bubo, hernia and tinea. Cinnamon: tonify primordial yang, warm spleen and stomach, remove cold accumulation, and promote blood circulation. Chinese yam: has the effects of nourishing and strengthening body, promoting digestion, astringing sweating due to debility and stopping diarrhea. E, donkey-hide gelatin: tonify blood, nourish yin, moisten dryness and stop bleeding. Can be used for treating blood deficiency, sallow complexion, giddiness, palpitation, vexation, insomnia, lung dryness and cough. Wolfberry fruit: nourishing liver and kidney, replenishing vital essence and improving eyesight. Mulberry: mulberry is sweet and cold in nature, and has the effects of tonifying liver and kidney, promoting fluid production and moistening dryness, blackening hair and improving eyesight and the like. Fructus alpiniae oxyphyllae: has the effects of warming spleen, stopping diarrhea, controlling saliva, warming kidney, reducing urination and stopping emission. Rhizoma polygonati: has effects in invigorating spleen, moistening lung, and promoting salivation. Raspberry: has the functions of invigorating kidney, stopping nocturnal emission, reducing urination, nourishing liver and improving eyesight.

The traditional Chinese medicine components are reasonably compatible and act synergistically, so that the traditional Chinese medicine composition has good effects of tonifying kidney and strengthening yang, and is obvious in effect and small in toxic and side effects.

Preferably, the herbal composition consists of the following traditional Chinese medicine components in parts by weight: 5-7 parts of clove, 2-4 parts of cinnamon, 25-35 parts of Chinese yam, 9-12 parts of donkey-hide gelatin, 15-18 parts of wolfberry fruit, 15-20 parts of mulberry, 12-17 parts of fructus alpiniae oxyphyllae, 12-18 parts of rhizoma polygonati and 15-20 parts of raspberry.

Preferably, the alcoholic strength of the herbal wine is 35-37 vol%.

A preparation method of herbal wine with kidney invigorating and yang strengthening effects comprises the following steps:

(1) mixing flos Caryophylli, cortex Cinnamomi, rhizoma Dioscoreae, fructus Lycii, Mori fructus, fructus Alpinae Oxyphyllae, rhizoma Polygonati and Rubi fructus, pulverizing, soaking in Chinese liquor for 1-3 months, and filtering to obtain extractive solution;

(2) melting colla Corii Asini, mixing with the leaching solution, adding purified water, and reducing to obtain wine solution;

(3) standing the liquor for more than 30 days, sieving the supernatant with 200 mesh sieve, packaging the filtrate into wine bottles, testing under light, and packaging.

Preferably, in the step (1), the alcoholic strength of the white spirit is 37-42 vol%.

Preferably, the white spirit is Maotai-flavor white spirit.

Preferably, in step (1), 400-600mL of white spirit is added to 100g of the crushed material for soaking.

Preferably, in the step (2), the alcoholic strength of the wine liquid after adding the purified water and reducing the alcoholic strength is 35-37 vol%.

The invention has the beneficial effects that: the traditional Chinese medicine components are reasonably compatible and act synergistically, so that the traditional Chinese medicine composition has good effects of tonifying kidney and strengthening yang, and is obvious in effect and small in toxic and side effects.

Drawings

FIG. 1 rat status observation.

Figure 2 effect of herbal wine on rat nutrition profile, note: p <0.01 to normal control group; compared with the kidney-yang deficiency model group, # P < 0.01.

Figure 3 effect of herbal alcohol on liver function, note: p <0.05 compared to normal control group.

Figure 4 effect of herbal wine on kidney function, note: p <0.01 to normal control group; compared with the kidney-yang deficiency model group, # P < 0.01.

Figure 5 effect of herbal wine on rat uric acid, note: p <0.001 to normal control group.

Figure 6 effect of herbal wine on rat T3, T4, note: p <0.05, P <0.01 to normal control group; compared with the kidney-yang deficiency model group, the ratio of the # P is less than 0.05.

Figure 7 effect of herbal alcohol on rat sex hormones, note: p <0.05 compared to normal control group; compared with the kidney-yang deficiency model group, # P <0.05, # # P < 0.001.

FIG. 8 the effect of herbal alcohol on rat liver tissue.

Figure 9 effect of herbal alcohol on rat testicular tissue.

FIG. 10 is a process flow diagram of the present invention.

Detailed Description

The technical solution of the present invention will be further specifically described below by way of specific examples.

In the present invention, the raw materials and equipment used are commercially available or commonly used in the art, unless otherwise specified. The methods in the following examples are conventional in the art unless otherwise specified.

Example 1:

a herbal wine with kidney invigorating and yang supporting effects is prepared by the following method (as shown in figure 10):

(1) mixing and crushing 4 parts by weight of clove, 4 parts by weight of cinnamon, 20 parts by weight of Chinese yam, 20 parts by weight of medlar, 15 parts by weight of mulberry, 20 parts by weight of sharpleaf galangal fruit, 12 parts by weight of rhizoma polygonati and 25 parts by weight of raspberry, adding Maotai-flavor liquor (sold in the market) with the liquor accuracy of 37 vol% into the crushed materials, soaking for 3 months, and filtering to obtain a leaching solution; each 100g of the crushed material is soaked in 600mL of white spirit.

(2) Melting 8 parts by weight of donkey-hide gelatin, uniformly mixing with the leaching liquor, and then adding purified water to reduce the alcohol content to 35% to obtain wine liquor.

(3) Standing the liquor for more than 30 days, taking the supernatant, sieving with a 200-mesh sieve, subpackaging the filtrate into wine bottles with 120 ml/bottle, performing light inspection, packaging, and inspecting to obtain the finished product.

Example 2:

a herbal wine with kidney-tonifying and yang-strengthening effects is prepared by the following method:

(1) mixing 8 parts by weight of clove, 2 parts by weight of cinnamon, 40 parts by weight of Chinese yam, 10 parts by weight of medlar, 20 parts by weight of mulberry, 10 parts by weight of sharpleaf galangal fruit, 18 parts by weight of rhizoma polygonati and 15 parts by weight of raspberry, crushing, adding soy sauce flavor type white spirit (sold in the market) with the wine accuracy of 42 vol% into the crushed materials, soaking for 1 month, and filtering to obtain a leaching solution; 400mL of white spirit is added to each 100g of the crushed material for soaking.

(2) Melting 15 parts by weight of donkey-hide gelatin, uniformly mixing the donkey-hide gelatin with the leaching liquor, and then adding purified water to reduce the alcohol content to 37 vol% to obtain liquor.

Example 3:

(1) mixing 6 parts by weight of clove, 3 parts by weight of cinnamon, 30 parts by weight of Chinese yam, 15 parts by weight of medlar, 15 parts by weight of mulberry, 15 parts by weight of sharpleaf galangal fruit, 15 parts by weight of rhizoma polygonati and 15 parts by weight of raspberry, crushing, adding soy sauce flavor type white spirit (sold in the market) with the wine accuracy of 40 vol% into the crushed materials, soaking for 2 months, and filtering to obtain a leaching solution; each 100g of the crushed material is soaked in 500mL of white spirit.

(2) Melting 10 parts by weight of donkey-hide gelatin, uniformly mixing with the leaching liquor, and then adding purified water to reduce the alcohol content to 36 vol% to obtain wine liquor.

The experimental study on the influence of the herbaceous plant wine on normal and kidney-yang-deficient rats comprises the following steps:

1 study protocol:

male SD rats 64 were randomly divided into 8 groups of 8 rats, and were kept at room temperature with controlled air humidity, ad libitum drinking and eating.

A, feeding normal control group with normal saline with the maximum intragastric administration volume equivalent to that of the treatment group;

b herbal wine treatment group (low): continuously feeding the mixture with low-dose herbaceous plant wine for 4 weeks;

(rat standard dose 1/2 converted according to normal alcohol content)

C herbal wine treatment group (middle): feeding standard dose of herbal wine

(according to the alcoholic strength, converting the normal wine capacity of normal people into rat standard dose) continuously feeding for 4 weeks;

herbal wine treatment group (high): feeding high-dose herbaceous plant wine

(according to the alcoholic strength, converting the standard dosage of rats by referring to the normal wine capacity of normal people by 3), and continuously feeding for 4 weeks;

e, in the kidney-yang deficiency model group, the hormone is removed after continuously injecting hydrocortisone injection with the weight of 25mg ∙ kg-1 for 8 days;

herbal wine treatment group (low): removing hormone after continuously injecting hydrocortisone injection at 25mg ∙ kg-1 body weight for 8 days; feeding herbal wine with standard dosage for treatment, and continuously feeding for 4 weeks;

(rat standard dose 1/2 converted according to normal alcohol content)

G herbal wine treatment group (middle): removing hormone after continuously injecting hydrocortisone injection at 25mg ∙ kg-1 body weight for 8 days; feeding low-dose herbal wine (according to alcohol content, converting into rat standard dose according to normal wine amount of normal person) for treatment, and continuously feeding for 4 weeks;

h herbal wine treatment group (high): feeding high-dose herbaceous plant wine

(according to the alcoholic strength, the standard dosage of rats is converted by normal wine amount of normal people 3) and the treatment is carried out, and the feeding is continuously carried out for 4 weeks.

After the model is successfully made, the herbaceous plant wine is continuously fed for 4 weeks, the state, the excrement and urine and the change situation of the activity of the rat are observed every day, and the weight is measured once a week. All rats were sacrificed at the end of week 4.

The detection indexes are as follows:

(1) blood is taken from the vena cava under anesthesia, serum is separated, blood biochemical indexes such as total serum protein (TP), Albumin (ALB), aspartate Aminotransferase (AST), alanine Aminotransferase (ALT), urea nitrogen (BUN), creatinine (CRea), Uric Acid (UA) and the like are detected, and the change of the liver function and the kidney function of each group of rats is observed.

(2) The kit is used for detecting the contents of testosterone, estradiol, thyroxine T3 and T4 in serum, and determining the influence of the herbaceous plant wine on the reproductive system and energy metabolism of the rats with kidney-yang deficiency.

(3) After formalin-fixed samples of liver tissues and testis tissues, HE staining is carried out, and histopathological changes are observed.

And finally, analyzing and discussing the experimental result to finally obtain a conclusion.

2, experimental results:

2.1 rat status observations:

the rats in the normal group move freely, have normal defecation and urination, and have normal drinking water and diet. After the model is made by hydrocortisone, the spirit of the rat is weakened, the activity is reduced, the stool is thin and not formed, and the symptoms of kidney-yang deficiency such as arch back, hair sparseness and the like appear.

2.2 Effect of herbal wines on rat weight

The rats in each group before modeling are randomly distributed, so the body weight of each group is not different, but after hydrocortisone modeling, the body weight of each group of model rats is obviously lower than that of the normal rats (P < 0.001). After the treatment of the herbal wine, the weight gain of the low dose is more than that of the model group, and the weight gain of the high dose and medium dose groups is not obvious. There was no significant difference between the body weights of the groups of rats that were not modeled (table 1).

TABLE 1 rat body weight changes

。

2.3 Effect of herbal wines on rat nutritional status

After the rats in the normal group are perfused with the herbal wine with low, medium and high concentrations, Total Protein (TP) and white protein (ALB) in blood have no obvious change, which indicates that the herbal wine has no obvious influence on the nutrition condition of the normal rats. After hydrocortisone molding, the content of TP and ALB is obviously reduced (P is less than 0.05), which indicates that the body energy metabolism of animals with kidney-yang deficiency is obstructed and the nutritional state is poor, so the animals are in the states of hunch back rise, aversion to cold and cold limbs. After the herbaceous plant wine is infused, the levels of TP and ALB are obviously increased, which shows that the herbaceous plant wine is helpful for improving the nutritional status of rats with kidney-yang deficiency and increasing the nutritional level of rats (figure 2).

2.4 influence of herbal wine on liver function:

no matter the rats in the normal group are filled with the herbal wine with low, medium and high concentrations, the indexes of glutamic-oxaloacetic transaminase (AST) and glutamic-pyruvic transaminase (ALT) in blood have no obvious change, which shows that the herbal wine has no obvious influence on the liver function of the normal rats. After the rat is treated by the herbal wine, the AST value of the rat is reduced, but the difference is not obvious, compared with a model group after the rat is treated by the herbal wine after the rat is infused with the herbal wine. It is demonstrated that drinking herbal wine for 4 weeks had no significant effect on liver function (fig. 3).

2.5 Effect of herbal wines on Kidney function

No matter the rats in the normal group are filled with the herbal wine with low, medium and high concentrations, the Creatinine (CREA) and urea nitrogen (BUN) indexes in the blood have no obvious change, which indicates that the herbal wine has no obvious influence on the renal function of the normal rats. After hydrocortisone modeling, the CREA and BUN are obviously increased, which indicates that kidney-yang deficiency animals have low renal function, while the CREA and BUN content after gastric lavage of herbal wine is reduced to the greatest extent in a high-concentration group (P is less than 0.01), which indicates that the herbal wine can obviously improve the renal function of rats with kidney-yang deficiency (figure 4).

2.6 Effect of herbal wines on uric acid

The Uric Acid (UA) level of normal rats was significantly reduced (P <0.001) after taking the herbal alcohol at both low and medium concentrations, but was not significantly changed in the high concentration group. For rats with kidney-yang deficiency, the effect of herbal alcohol in reducing uric acid was not obvious (fig. 5).

2.7 Effect of herbal wines on thyroxine

In the normal group, the herbaceous plant wine has little influence on thyroxine T3 and T4, but the levels of T3 and T4 in the serum of rats after hydrocortisone molding are obviously reduced (P is less than 0.05), and the thyroxine level is increased after the rats are subjected to gastric lavage by the herbaceous plant wine. Thyroxine can generate heat through uncoupling of mitochondrial respiratory chain, promote metabolism of sugar, protein and the like, and has important effects on organism productivity and growth and development. The reduction of thyroxine in the model group indicates that the model accords with the general kidney-yang deficiency, the T3 and T4 levels can be improved after the model is fed with herbal wine, and the herbal wine has a certain treatment effect on rats with kidney-yang deficiency (figure 6).

2.8 Effect of herbal wines on sex hormones

The content of estradiol in each group of rats has no obvious change; the change of each group on the content of testosterone in serum is obvious, and the content of testosterone in the herb plant wine in the gavage is obviously increased (P is less than 0.05) in normal rats; in rats of a model group injected with hydrocortisone, the testosterone content is remarkably reduced (P is less than 0.05), which is consistent with the clinical symptoms of kidney-yang deficiency, and after the rats are infused with herbal wine, the testosterone level is remarkably improved, which indicates that the herbal wine can improve the androgen level and remarkably improve the symptoms of kidney-yang deficiency (figure 7).

2.9 Effect of herbaceous plant wine on pathological changes of liver tissue of rat with kidney-yang deficiency

The HE (400X) staining of the liver of each group of rats showed no significant change in liver morphological function after administration of herbal wine to normal and model rats (FIG. 8).

2.10 Effect of herbal wine on Kidney Yang deficiency in rat testis tissue

As can be seen from HE staining (100 ×), the rat convoluted tubule of the model group has obvious atrophy degeneration, and the number of interstitial cells and sperm cells is obviously reduced compared with the normal group. In the normal group, it can be seen that the sperm cells at all levels in the seminal tubules are well developed, the number of the sperm cells is large, the arrangement is compact, the cavities are filled with sperms, the boundaries of the tube walls are neat, the basement membrane is thin, and the development of the testicular interstitial cells is normal. After the herbaceous plant wine is infused, the normal rat does not have obvious change, but the model rat can obviously change, the number of seminal tubules and sperms is increased, and the arrangement is more compact (figure 9).

3. Conclusion of the experiment

1. After the glucocorticoid hydrocortisone model is made, the weight of a rat is obviously reduced, and a series of symptoms such as loose stool, reduced activity, shrugging of the back and the like appear, which indicates that the construction of the kidney-yang deficiency model is successful.

2. After the rats are fed with the herbal wine, the weight, the nutritional status and the liver function of the rats are not obviously changed, which indicates that the herbal wine has no obvious toxic or side effect on normal organisms, and does not change in a high-dose group, and indicates that the herbal wine is very safe in a dose range.

3. After the model is established, the two indexes of the kidney function, namely urea nitrogen and blood creatinine, are obviously increased, which is the specific embodiment of the kidney function degradation; meanwhile, the levels of thyroxine T3 and T4 which represent two indexes of energy metabolism are obviously reduced, which indicates that the metabolism of a model rat is reduced and the productivity is reduced, so that the cold intolerance symptom is caused, and the levels of blood creatinine and urea nitrogen are obviously reduced after the herbal wine is fed, which indicates that the herbal wine has the function of enhancing the kidney function, and simultaneously, the contents of thyroxine T3 and T4 are also increased, which indicates that the herbal wine can improve the metabolic rate of an organism.

4. It can be seen from the level of male hormone testosterone and HE staining of testis that the reproductive function of model group rat is obviously reduced compared with normal rat, and after 4 weeks of herbaceous plant liquor gavage, the content of serum testosterone is obviously improved, and reproductive function is obviously enhanced.

5. In addition, uric acid level is one of the indexes for measuring kidney function, and the increase of uric acid level is a high-risk factor of various diseases including gout.

6. Most indexes have obvious change in low concentration and medium concentration groups, and the dosage of the index is converted from the daily recommended drinking amount of normal people, so that proper drinking is recommended.

The above-described embodiments are only preferred embodiments of the present invention, and are not intended to limit the present invention in any way, and other variations and modifications may be made without departing from the spirit of the invention as set forth in the claims.

Claims

1. The herbal wine with the effects of tonifying kidney and strengthening yang is characterized by being prepared from a herbal composition, wherein the herbal composition comprises the following traditional Chinese medicine components in parts by weight: 4-8 parts of clove, 2-4 parts of cinnamon, 20-40 parts of Chinese yam, 8-15 parts of donkey-hide gelatin, 10-20 parts of wolfberry fruit, 15-20 parts of mulberry, 10-20 parts of fructus alpiniae oxyphyllae, 12-18 parts of rhizoma polygonati and 15-25 parts of raspberry.

2. The herbal wine with the effects of tonifying kidney and strengthening yang as claimed in claim 1, wherein the herbal composition comprises the following traditional Chinese medicine components in parts by weight: 5-7 parts of clove, 2-4 parts of cinnamon, 25-35 parts of Chinese yam, 9-12 parts of donkey-hide gelatin, 15-18 parts of wolfberry fruit, 15-20 parts of mulberry, 12-17 parts of fructus alpiniae oxyphyllae, 12-18 parts of rhizoma polygonati and 15-20 parts of raspberry.

3. The herbal wine having the effects of invigorating kidney and strengthening yang as claimed in claim 1 or 2, wherein the alcohol content of the herbal wine is 35-37 vol%.

4. A method for preparing herbal wine having kidney-invigorating and yang-strengthening effects as claimed in claim 1, comprising the steps of:

5. The preparation method according to claim 4, wherein in the step (1), the alcoholic strength of the white spirit is 37-42 vol%.

6. The method according to claim 5, wherein the liquor is Maotai-flavor liquor.

7. The method according to claim 4, wherein in the step (1), 400mL of distilled spirit is added to 100g of the pulverized material for soaking.

8. The method according to claim 4, wherein the alcohol content of the alcohol solution after the alcohol content is reduced by adding purified water in the step (2) is 35 to 37 vol%.