CN106852153A8 - 具有广谱活性的杀昆虫多肽和其用途 - Google Patents

具有广谱活性的杀昆虫多肽和其用途 Download PDF

Info

Publication number
CN106852153A8
CN106852153A8 CN201580056324.XA CN201580056324A CN106852153A8 CN 106852153 A8 CN106852153 A8 CN 106852153A8 CN 201580056324 A CN201580056324 A CN 201580056324A CN 106852153 A8 CN106852153 A8 CN 106852153A8
Authority
CN
China
Prior art keywords
activity
killed
purposes
insect
broad spectrum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201580056324.XA
Other languages
English (en)
Other versions
CN106852153B (zh
CN106852153A (zh
Inventor
A.R.阿巴德
A.C.克劳
B.波兰德
X.施
T.C.沃尔菲
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Hi Bred International Inc
Original Assignee
Pioneer Hi Bred International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred International Inc filed Critical Pioneer Hi Bred International Inc
Publication of CN106852153A publication Critical patent/CN106852153A/zh
Publication of CN106852153A8 publication Critical patent/CN106852153A8/zh
Application granted granted Critical
Publication of CN106852153B publication Critical patent/CN106852153B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50Isolated enzymes; Isolated proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04Plant cells or tissues
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pest Control & Pesticides (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Insects & Arthropods (AREA)
  • Environmental Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Dentistry (AREA)
  • Virology (AREA)
  • Agronomy & Crop Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

本发明提供了从苏云金芽孢杆菌菌株获得的编码具有对昆虫害虫(包括鳞翅目和鞘翅目昆虫)的杀虫活性的多肽的核酸及其变体和片段。本发明的具体实施方案提供编码杀虫蛋白的分离核酸、包含本发明实施方案的核酸的杀虫组合物、DNA构建体及转化的微生物和植物。这些组合物可在用于防治害虫特别是植物害虫的方法中使用。
CN201580056324.XA 2014-10-16 2015-10-09 具有广谱活性的杀昆虫多肽和其用途 Active CN106852153B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462064848P 2014-10-16 2014-10-16
US62/064,848 2014-10-16
PCT/US2015/054869 WO2016060949A1 (en) 2014-10-16 2015-10-09 Insecticidal polypeptides having broad spectrum activity and uses thereof

Publications (3)

Publication Number Publication Date
CN106852153A CN106852153A (zh) 2017-06-13
CN106852153A8 true CN106852153A8 (zh) 2017-07-14
CN106852153B CN106852153B (zh) 2023-06-06

Family

ID=55747163

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201580056324.XA Active CN106852153B (zh) 2014-10-16 2015-10-09 具有广谱活性的杀昆虫多肽和其用途

Country Status (11)

Country Link
US (1) US10480008B2 (zh)
EP (1) EP3207126A4 (zh)
CN (1) CN106852153B (zh)
BR (1) BR112017007818B1 (zh)
CA (1) CA2963550A1 (zh)
MX (1) MX369364B (zh)
PH (1) PH12017500665A1 (zh)
RU (1) RU2750459C2 (zh)
UA (1) UA124757C2 (zh)
WO (1) WO2016060949A1 (zh)
ZA (1) ZA201702168B (zh)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8839920B2 (en) 2008-04-17 2014-09-23 Levant Power Corporation Hydraulic energy transfer
MX2018015906A (es) 2016-07-01 2019-04-04 Pioneer Hi Bred Int Proteinas insecticidas de plantas y metodos para sus usos.
CN108795975B (zh) * 2018-07-04 2021-10-22 中国农业科学院油料作物研究所 野生大豆相关蛋白在提高植物抗虫性中的应用
US20230235352A1 (en) 2020-07-14 2023-07-27 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use

Family Cites Families (97)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ201918A (en) 1981-09-18 1987-04-30 Genentech Inc N-terminal methionyl analogues of bovine growth hormone
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
EP0192319B1 (en) 1985-01-22 1992-07-15 Mycogen Corporation Cellular encapsulation of biological pesticides
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US4853331A (en) 1985-08-16 1989-08-01 Mycogen Corporation Cloning and expression of Bacillus thuringiensis toxin gene toxic to beetles of the order Coleoptera
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US4873192A (en) 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
DE68929471D1 (de) 1988-09-06 2003-07-10 Bayer Bioscience Nv Pflanzen, die mit einer lepidopter-lethalen DNS-Sequenz aus Bazillus thuringiensis transformiert werden
US5039523A (en) 1988-10-27 1991-08-13 Mycogen Corporation Novel Bacillus thuringiensis isolate denoted B.t. PS81F, active against lepidopteran pests, and a gene encoding a lepidopteran-active toxin
US5023179A (en) 1988-11-14 1991-06-11 Eric Lam Promoter enhancer element for gene expression in plant roots
US5110732A (en) 1989-03-14 1992-05-05 The Rockefeller University Selective gene expression in plants
US5240855A (en) 1989-05-12 1993-08-31 Pioneer Hi-Bred International, Inc. Particle gun
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
EP0400246A1 (en) 1989-05-31 1990-12-05 Plant Genetic Systems, N.V. Prevention of Bt resistance development
US5246852A (en) 1989-06-27 1993-09-21 Mycogen Corporation Bacillus thuringiensis isolate active against lepidopteran pests, and genes encoding novel lepidopteran-active toxins
US5188960A (en) * 1989-06-27 1993-02-23 Mycogen Corporation Bacillus thuringiensis isolate active against lepidopteran pests, and genes encoding novel lepidopteran-active toxins
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
EP0452269B1 (en) 1990-04-12 2002-10-09 Syngenta Participations AG Tissue-preferential promoters
CA2079877C (en) 1990-04-26 2001-07-31 Marnix Peferoen Bacillus thuringiensis strains and their genes encoding insecticidal toxins
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
CA2051562C (en) 1990-10-12 2003-12-02 Jewel M. Payne Bacillus thuringiensis isolates active against dipteran pests
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
US5277905A (en) 1991-01-16 1994-01-11 Mycogen Corporation Coleopteran-active bacillus thuringiensis isolate
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
ES2124738T3 (es) 1991-08-02 1999-02-16 Mycogen Corp Nuevo microorganismo e insecticida.
HU219057B (hu) 1991-08-27 2001-02-28 Novartis Ag. Inszekticid hatású proteinek Homoptera rovarok ellen és alkalmazásuk a növényvédelemben
JPH06511152A (ja) 1991-10-04 1994-12-15 ノースカロライナ ステイト ユニバーシティー 病原体耐性トランスジェニック植物
TW261517B (zh) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
AU675923B2 (en) 1991-12-04 1997-02-27 E.I. Du Pont De Nemours And Company Fatty acid desaturase genes from plants
US5324646A (en) 1992-01-06 1994-06-28 Pioneer Hi-Bred International, Inc. Methods of regeneration of Medicago sativa and expressing foreign DNA in same
US5273746A (en) 1992-01-29 1993-12-28 Mycogen Corporation Bacillus thuringiensis isolates active against phthiraptera pests
US5428148A (en) 1992-04-24 1995-06-27 Beckman Instruments, Inc. N4 - acylated cytidinyl compounds useful in oligonucleotide synthesis
US5401836A (en) 1992-07-16 1995-03-28 Pioneer Hi-Bre International, Inc. Brassica regulatory sequence for root-specific or root-abundant gene expression
EP0652965A1 (en) 1992-07-27 1995-05-17 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
WO1994011516A1 (en) 1992-11-17 1994-05-26 E.I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and related enzymes from plants
CA2127807A1 (en) 1992-11-20 1994-06-09 John Maliyakal Transgenic cotton plants producing heterologous bioplastic
IL108241A (en) 1992-12-30 2000-08-13 Biosource Genetics Corp Plant expression system comprising a defective tobamovirus replicon integrated into the plant chromosome and a helper virus
DE69428290T2 (de) 1993-01-13 2002-04-18 Pioneer Hi Bred Int Derivate von alpha-hordothionin mit höherem behalt an lysin
US5583210A (en) 1993-03-18 1996-12-10 Pioneer Hi-Bred International, Inc. Methods and compositions for controlling plant development
US5849870A (en) 1993-03-25 1998-12-15 Novartis Finance Corporation Pesticidal proteins and strains
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5593881A (en) 1994-05-06 1997-01-14 Mycogen Corporation Bacillus thuringiensis delta-endotoxin
US5633363A (en) 1994-06-03 1997-05-27 Iowa State University, Research Foundation In Root preferential promoter
US5530195A (en) 1994-06-10 1996-06-25 Ciba-Geigy Corporation Bacillus thuringiensis gene encoding a toxin active against insects
US5736369A (en) 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5792931A (en) 1994-08-12 1998-08-11 Pioneer Hi-Bred International, Inc. Fumonisin detoxification compositions and methods
EP0711834A3 (en) 1994-10-14 1996-12-18 Nissan Chemical Ind Ltd New Bacillus strain and product for the control of harmful organisms
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
EP0832235A1 (en) 1995-06-02 1998-04-01 Pioneer Hi-Bred International, Inc. HIGH METHIONINE DERIVATIVES OF alfa-HORDOTHIONIN
AU705933B2 (en) 1995-06-02 1999-06-03 Pioneer Hi-Bred International, Inc. High threonine derivatives of alpha-hordothionin
US5837876A (en) 1995-07-28 1998-11-17 North Carolina State University Root cortex specific gene promoter
US5981722A (en) 1995-11-20 1999-11-09 Board Of Regents For The University Of Oklahoma Trypsin inhibitors with insecticidal properties obtained from PENTACLETHRA MACROLOBA
US5703049A (en) 1996-02-29 1997-12-30 Pioneer Hi-Bred Int'l, Inc. High methionine derivatives of α-hordothionin for pathogen-control
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
JP3441899B2 (ja) 1996-11-01 2003-09-02 理化学研究所 完全長cDNAライブラリーの作成方法
US6232529B1 (en) 1996-11-20 2001-05-15 Pioneer Hi-Bred International, Inc. Methods of producing high-oil seed by modification of starch levels
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
WO1999025855A1 (en) 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Mobilization of viral genomes from t-dna using site-specific recombination systems
EP1032692A1 (en) 1997-11-18 2000-09-06 Pioneer Hi-Bred International, Inc. Targeted manipulation of herbicide-resistance genes in plants
DE69833457T2 (de) 1997-11-18 2006-09-14 Pioneer Hi-Bred International, Inc. Neuartige methode zur integration von fremd-dna ins eukaryotische genom
AU760113C (en) 1997-11-18 2004-04-22 Pioneer Hi-Bred International, Inc. Compositions and methods for genetic modification of plants
CA2315549A1 (en) 1998-02-26 1999-09-02 Pioneer Hi-Bred International, Inc. Family of maize pr-1 genes and promoters
AU762993C (en) 1998-02-26 2004-06-10 Pioneer Hi-Bred International, Inc. Constitutive maize promoters
WO1999061619A2 (en) 1998-05-22 1999-12-02 Pioneer Hi-Bred International, Inc. Cell cycle genes, proteins and uses thereof
DE69928264T2 (de) 1998-08-20 2006-07-13 Pioneer Hi-Bred International, Inc. Samen-bevorzugende promotoren
WO2000012733A1 (en) 1998-08-28 2000-03-09 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from end genes
US6518487B1 (en) 1998-09-23 2003-02-11 Pioneer Hi-Bred International, Inc. Cyclin D polynucleotides, polypeptides and uses thereof
US6489542B1 (en) * 1998-11-04 2002-12-03 Monsanto Technology Llc Methods for transforming plants to express Cry2Ab δ-endotoxins targeted to the plastids
IL142736A0 (en) 1998-11-09 2002-03-10 Pioneer Hi Bred Int Transcriptional activator lec 1 nucleic acids, polypeptides and their uses
US7462481B2 (en) 2000-10-30 2008-12-09 Verdia, Inc. Glyphosate N-acetyltransferase (GAT) genes
US6858778B1 (en) 2000-11-07 2005-02-22 Pioneer Hi-Bred International, Inc. Plants transformed with a DNA construct comprising a nucleic acid molecule encoding an 18 kD α-globulin
US7009087B1 (en) 2000-12-01 2006-03-07 Pioneer Hi-Bred International, Inc. Compositions and methods for altering the disulfide status of proteins
WO2004003148A2 (en) 2002-06-26 2004-01-08 E.I. Du Pont De Nemours And Company Genes encoding proteins with pesticidal activity
US7462760B2 (en) 2002-06-26 2008-12-09 Pioneer Hi-Bred International, Inc. Genes encoding plant protease-resistant pesticidal proteins and method of their use
US7449552B2 (en) * 2006-04-14 2008-11-11 Pioneer Hi-Bred International, Inc. Bacillus thuringiensis cry gene and protein
CN103937815A (zh) * 2008-01-10 2014-07-23 先锋国际良种公司 具有鞘翅目活性的新的苏云金芽孢杆菌基因
US8269069B1 (en) * 2008-07-30 2012-09-18 Dow Agrosciences, Llc Modified Bacillus thuringiensis cry proteins that inhibit coleopterans
CA2780540A1 (en) * 2009-11-12 2011-05-19 Pioneer Hi-Bred International, Inc. Novel bacillus thuringiensis gene with lepidopteran activity
CA2825951C (en) * 2011-02-11 2019-08-20 Monsanto Technology Llc Pesticidal nucleic acids and proteins and uses thereof
CN102675438B (zh) * 2011-03-18 2015-04-22 中国科学院上海生命科学研究院 基于RNAi技术的抗虫制剂及方法
CA2866239C (en) * 2012-03-08 2020-07-28 Athenix Corp. Bacillus thuringiensis toxin gene axmi335 and methods for its use
US9567381B2 (en) 2012-03-09 2017-02-14 Vestaron Corporation Toxic peptide production, peptide expression in plants and combinations of cysteine rich peptides
CA2886787A1 (en) 2012-10-05 2014-04-10 Dow Agrosciences Llc Use of cry1ea in combinations for management of resistant fall armyworm insects
US10077171B2 (en) * 2012-10-17 2018-09-18 Grant C. Chustz Safety barrier for vehicles and cranes
AU2014225732B2 (en) * 2013-03-07 2020-03-19 BASF Agricultural Solutions Seed US LLC Toxin genes and methods for their use
CN103333230B (zh) 2013-07-09 2016-02-17 福建农林大学 苏云金芽孢杆菌基因cry1Da3及其应用
AR099800A1 (es) 2014-03-21 2016-08-17 Agrigenetics Inc Cry1d para controlar el gusano de la mazorca del maíz
CN104673706B (zh) 2014-12-20 2018-03-06 东北农业大学 苏云金芽胞杆菌fh21、杀虫基因,表达蛋白及其应用

Also Published As

Publication number Publication date
RU2017116797A (ru) 2018-11-16
ZA201702168B (en) 2018-05-30
CN106852153B (zh) 2023-06-06
BR112017007818B1 (pt) 2022-12-13
US10480008B2 (en) 2019-11-19
RU2750459C2 (ru) 2021-06-28
EP3207126A1 (en) 2017-08-23
UA124757C2 (uk) 2021-11-17
MX369364B (es) 2019-11-06
US20170233759A1 (en) 2017-08-17
RU2017116797A3 (zh) 2018-12-13
EP3207126A4 (en) 2018-04-04
CA2963550A1 (en) 2016-04-21
MX2017004744A (es) 2017-07-27
BR112017007818A2 (pt) 2017-12-19
CN106852153A (zh) 2017-06-13
PH12017500665A1 (en) 2017-10-02
WO2016060949A1 (en) 2016-04-21

Similar Documents

Publication Publication Date Title
PH12017500679A1 (en) Insecticidal polypeptides having improved activity spectrum and uses thereof
PH12021500020A1 (en) Novel chimeric insecticidal proteins toxic or inhibitory to lepidopteran pests
PH12016500230A1 (en) Insecticidal polypeptides having broad spectrum activity and uses thereof
MX350329B (es) Gen de bacillus thuringiensis novedoso con actividad lepidoptera.
MX2013008392A (es) Nuevos genes de bacillus thuringiensis con actividad lepidoptera.
WO2012024200A3 (en) Novel bacillus thuringiensis gene with lepidopteran activity
AR074509A1 (es) Gen de bacillus thuringiensis con actividad pesticida contra insectos del orden lepidoptera
AR078964A1 (es) Gen de bacillus thuringiensis que codifica polipeptido con actividad pesticida contra lepidoptera
NZ706821A (en) Brevibacillus laterosporus strains nmi no. v1200945, nmi no. v1200946, and nmi no. v1200947, as biocontrol agents against plant pests, particularly lepidoptera and diptera
PH12017501018A1 (en) Composition and methods for controlling plant pests
WO2012024176A3 (en) Novel bacillus thuringiensis gene with lepidopteran activity
WO2016061377A3 (en) Lepidopteran-active cry1da1 amino acid sequence variant proteins
CN106852153A8 (zh) 具有广谱活性的杀昆虫多肽和其用途
PH12017501010A1 (en) Compositions and methods for controling plant pests
WO2012024372A3 (en) Novel bacillus thuringiensis gene with coleopteran activity
PH12018501814A1 (en) Insecticidal polypeptides having improved activity spectrum and uses thereof
BR112017007930A2 (pt) polipeptídeos inseticidas tendo atividades de amplo espectro e usos dos mesmos
EA201992824A1 (ru) Инсектицидные полипептиды, обладающие улучшенным спектром активности, и пути их применения
WO2017199078A3 (en) Bacillus thuringiensis cyt1a mutants

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
CI01 Publication of corrected invention patent application
CI01 Publication of corrected invention patent application

Correction item: Priority

Correct: 62/064,848 2014.10.16 US

Number: 24-01

Volume: 33

CI02 Correction of invention patent application
CI02 Correction of invention patent application

Correction item: Priority

Correct: 62/064,848 2014.10.16 US

Number: 24-01

Page: The title page

Volume: 33

GR01 Patent grant
GR01 Patent grant