CN106822844A

CN106822844A - A kind of Chinese medicine composition of prevention glioma postoperative recurrence

Info

Publication number: CN106822844A
Application number: CN201710156755.4A
Authority: CN
Inventors: 徐华明; 韩德恩; 杨念; 申延利; 辛玉凤; 苗玉芳
Original assignee: Henan University of Traditional Chinese Medicine HUTCM
Current assignee: Henan University of Traditional Chinese Medicine HUTCM
Priority date: 2017-03-16
Filing date: 2017-03-16
Publication date: 2017-06-13
Also published as: CN113967244A

Abstract

Chinese medicine composition the present invention relates to prevent glioma postoperative recurrence, can effectively be solved the problems, such as to prevent the medication of glioma postoperative recurrence, and method is made up of the material medicine of following parts by weight meter：18 parts of Radix Codonopsis, 15 parts of the root of large-flowered skullcap, 15 parts of Os Draconis, 15 parts of raw oyster, 1 15 parts of cassia twig, 1 10 parts of Poria cocos, 15 parts of prepared RHIZOMA PINELLIZE without adju-vant, 18 parts of radix bupleuri, 1 15 parts of rheum officinale, fry two ugly 15 parts, 17 parts of moutan bark, 1 15 parts of gypsum, 15 parts of gypsum rubrum, 17 parts of rhizoma zingiberis, 17 parts of great Bei, 1 10 parts of radix scrophulariae, 15 parts of 18 parts of licorice and date, instant component is scientific and reasonable, preparation method is simple, it is easy to operate, low cost, it is convenient to take, effect is good, it is prepared by easily production, it is to prevent the innovation on glioma postoperative recurrence medicine, there is good economic and social benefit.

Description

A kind of Chinese medicine composition of prevention glioma postoperative recurrence

Technical field

The present invention relates to medicine, particularly a kind of Chinese medicine composition of prevention glioma postoperative recurrence.

Background technology

Glioma is the primary tumor caused by brain and spinal cord spongiocyte canceration, be central nervous system the most One of common tumour, accounts for the 30-50% of intracranial tumour, and with the incidence of disease is high, high recurrence rate, the death rate be high, cure rate is low Feature, its pathogenesis etiology still indefinite so far.Chinese glioma annual morbidity is 3-6 people/100,000 people, is fallen ill in recent years In rejuvenation trend, at 30-40 Sui, children's morbidity in 10 years old or so is also more in addition on age of onset peak.

To glioma still with surgery excision as essential therapeutic arsenals, supplemented by radiotherapy, chemotherapy, but because glioma is in The unrestricted hyperplasia of existing wellability, shortage apoptosis, and without obvious boundary between normal cerebral tissue, multiple cerebral lobes are invaded, or even invade Brain deep critical function area, it is thorough that operation is difficult to excision；Again because brain glioblastoma cell is insensitive to ray, chemotherapeutics is again difficult Through blood-brain barrier and with drug resistance, to cause the clinical radiation therapy of glioma and chemotherapy effect undesirable, thus have Recurrence rate very high, 6 months mean survival time (MST)s, complex treatment life cycle was less than 2 years.Chinese medicine this disease has unique curative effect, Particularly prevention of postoperative recurrence.Traditional Chinese medicine thinks that this disease is mainly spleen kidney deficiency, wind phlegm condensation, ecchymosis internal resistance, healthy tendency virtual loss, many Treated with strengthening spleen, tonifying kidney method.But current surgery excision, radiotherapy, chemotherapy are the high method for attacking heresy fiercely, the healthy tendency that can be damaged patient More exhaustion, is unfavorable for recovery and the extension patient survival of disease.

Though the existing multi-medicament for having a Prevention glioma postoperative recurrence, for various reasons, particularly medicine Problem present on composition, it is used and unsatisfactory, therefore develops the medicine of new Prevention glioma postoperative recurrence It is imperative.

The content of the invention

For above-mentioned situation, for the purpose for overcoming the defect of prior art, the present invention is just to provide a kind of prevention brain colloid The Chinese medicine composition of knurl postoperative recurrence, can effectively solve the problems, such as to prevent the medication of glioma postoperative recurrence.

The technical scheme that the present invention is solved is that a kind of Chinese medicine composition for preventing glioma postoperative recurrence is by following weight The material medicine of number meter is made：Radix Codonopsis 1-8 parts, root of large-flowered skullcap 1-5 parts, Os Draconis 1-5 parts, raw oyster 1-5 parts, cassia twig 1-15 parts, Poria cocos 1-10 parts, prepared RHIZOMA PINELLIZE without adju-vant 1-5 parts, radix bupleuri 1-8 parts, rheum officinale 1-15 parts, fry two ugly 1-5 parts, moutan bark 1-7 parts, gypsum 1- 15 parts, gypsum rubrum 1-5 parts, rhizoma zingiberis 1-7 parts, great Bei 1-7 parts, radix scrophulariae 1-10 parts, licorice 1-8 parts and date 1-5 parts, wherein, To first be mixed except other 16 taste material medicines of Os Draconis, raw oyster, add other 16 taste material medicine weight 7-10 Water again, soaks 30-60min；Os Draconis, raw oyster are mixed, plus Os Draconis, the water of 7-10 times of raw oyster weight, Immersion 30-60min, decocts 30-40min, in other the 16 taste material medicines poured into after immersion 30-60min, then decocts 30- 40min, filtering, obtains first time filtrate；The dregs of a decoction add the water of 7-10 times of material medicine weight, decoct 30-40min, filter Second filtrate, merges filtrate twice, is mixed into Chinese medicine liquid, waits to take.

Instant component is scientific and reasonable, and preparation method is simple, and easy to operate, low cost is convenient to take, and effect is good, easily production Prepare, be to prevent the innovation on glioma postoperative recurrence medicine, there is good economic and social benefit.

Specific embodiment

Specific embodiment of the invention is elaborated with reference to embodiments.

The present invention can be realized in specific implementation by following examples.

Embodiment 1

In specific implementation, a kind of Chinese medicine composition for preventing glioma postoperative recurrence is by following parts by weight meter for the present invention Material medicine is made：7 parts of Radix Codonopsis, 4 parts of the root of large-flowered skullcap, 4 parts of Os Draconis, 4 parts of raw oyster, 8 parts of cassia twig, 8 parts of Poria cocos, 4 parts of prepared RHIZOMA PINELLIZE without adju-vant, bavin 6 parts recklessly, 9 parts of rheum officinale, ugly 4 parts of stir-fry two, 6 parts of moutan bark, 9 parts of gypsum, 4 parts of gypsum rubrum, 6 parts of rhizoma zingiberis, 5 parts of great Bei, radix scrophulariae 8 3 parts of part, 6 parts of licorice and date, wherein, will first be mixed except other 16 taste material medicines of Os Draconis, raw oyster, plus Enter the water of other 7-10 times of 16 taste material medicine weight, soak 30-60min；Os Draconis, raw oyster are mixed, plus it is raw The water of keel, 7-10 times of raw oyster weight, soaks 30-60min, decocts 30-40min, pours into other after immersion 30-60min In 16 taste material medicines, then 30-40min is decocted, filtered, obtain first time filtrate；The dregs of a decoction add 7-10 times of material medicine weight Water, decoct 30-40min, filter to obtain second filtrate, merge filtrate twice, be mixed into Chinese medicine liquid, wait to take.

Embodiment 2

In specific implementation, a kind of Chinese medicine composition for preventing glioma postoperative recurrence is by following parts by weight meter for the present invention Material medicine is made：5 parts of Radix Codonopsis, 6 parts of the root of large-flowered skullcap, 3 parts of Os Draconis, 3 parts of raw oyster, 5 parts of cassia twig, 3 parts of Poria cocos, 6 parts of prepared RHIZOMA PINELLIZE without adju-vant, bavin 4 parts recklessly, 5 parts of rheum officinale, ugly 6 parts of stir-fry two, 4 parts of moutan bark, 5 parts of gypsum, 6 parts of gypsum rubrum, 3 parts of rhizoma zingiberis, 3 parts of great Bei, radix scrophulariae 6 6 parts of part, 4 parts of licorice and date, preparation method is with embodiment 1.

Embodiment 3

In specific implementation, a kind of Chinese medicine composition for preventing glioma postoperative recurrence is by following parts by weight meter for the present invention Material medicine is made：3 parts of Radix Codonopsis, 4 parts of the root of large-flowered skullcap, 2 parts of Os Draconis, 2 parts of raw oyster, 4 parts of cassia twig, 4 parts of Poria cocos, 2 parts of prepared RHIZOMA PINELLIZE without adju-vant, bavin 3 parts recklessly, 4 parts of rheum officinale, ugly 3 parts of stir-fry two, 5 parts of moutan bark, 7 parts of gypsum, 3 parts of gypsum rubrum, 5 parts of rhizoma zingiberis, 3 parts of great Bei, radix scrophulariae 2 5 parts of part, 8 parts of licorice and date, preparation method is with embodiment 1.

Embodiment 4

In specific implementation, a kind of Chinese medicine composition for preventing glioma postoperative recurrence is by following parts by weight meter for the present invention Material medicine is made：2 parts of Radix Codonopsis, 3 parts of the root of large-flowered skullcap, 3 parts of Os Draconis, 3 parts of raw oyster, 2 parts of cassia twig, 2 parts of Poria cocos, 3 parts of prepared RHIZOMA PINELLIZE without adju-vant, bavin 3 parts recklessly, 2 parts of rheum officinale, ugly 2 parts of stir-fry two, 2 parts of moutan bark, 6 parts of gypsum, 2 parts of gypsum rubrum, 3 parts of rhizoma zingiberis, 2 parts of great Bei, radix scrophulariae 3 Part, 3 parts of licorice, 5 parts of date, preparation method is with embodiment 1.

Embodiment 5

In specific implementation, a kind of Chinese medicine composition for preventing glioma postoperative recurrence is by the raw material of following weight meter for the present invention Medicine is made：Radix Codonopsis 100g, root of large-flowered skullcap 100g, Os Draconis 300g, raw oyster 300g, cassia twig 100g, Poria cocos 120g, prepared RHIZOMA PINELLIZE without adju-vant 100g, radix bupleuri 100g, rheum officinale 100g, fry two ugly 60g, moutan bark 100g, gypsum 300g, gypsum rubrum 100g, rhizoma zingiberis 100g, greatly Shellfish 100g, radix scrophulariae 300g, licorice 60g and date 200g, preparation method is with embodiment 1.

Embodiment 6

In specific implementation, a kind of Chinese medicine composition for preventing glioma postoperative recurrence is by the raw material of following weight meter for the present invention Medicine is made：Radix Codonopsis 200g, root of large-flowered skullcap 200g, Os Draconis 100g, raw oyster 100g, cassia twig 200g, Poria cocos 150g, prepared RHIZOMA PINELLIZE without adju-vant 200g, radix bupleuri 200g, rheum officinale 200g, fry two ugly 100g, moutan bark 200g, gypsum 100g, gypsum rubrum 200g, rhizoma zingiberis 200g, Great Bei 200g, radix scrophulariae 100g, licorice 100g and date 100g, preparation method is with embodiment 1.

Embodiment 7

In specific implementation, a kind of Chinese medicine composition for preventing glioma postoperative recurrence is by the raw material of following weight meter for the present invention Medicine is made：Radix Codonopsis 50g, root of large-flowered skullcap 50g, Os Draconis 150g, raw oyster 150g, cassia twig 50g, Poria cocos 50g, prepared RHIZOMA PINELLIZE without adju-vant 60g, radix bupleuri 60g, rheum officinale 60g, fry two ugly 30g, moutan bark 50g, gypsum 150g, gypsum rubrum 50g, rhizoma zingiberis 50g, great Bei 60g, radix scrophulariae 150g, licorice 100g and date 80g, preparation method is with embodiment 1.

Embodiment 8

In specific implementation, a kind of Chinese medicine composition for preventing glioma postoperative recurrence is by the raw material of following weight meter for the present invention Medicine is made：Radix Codonopsis 30g, root of large-flowered skullcap 30g, Os Draconis 80g, raw oyster 80g, cassia twig 30g, Poria cocos 40g, prepared RHIZOMA PINELLIZE without adju-vant 40g, radix bupleuri 20g, rheum officinale 30g, fry two ugly 20g, moutan bark 30g, gypsum 50g, gypsum rubrum 40g, rhizoma zingiberis 30g, great Bei 30g, radix scrophulariae 20g, Licorice 30g and date 30g, preparation method is with embodiment 1.

In above-mentioned raw materials medicine, wherein：

Radix Codonopsis, is the dry root of Campanulaceae Radix Codonopsis, sweet, flat, returns spleen, lung channel；Strengthening spleen and tonifying lung, nourishes blood and promotes the production of body fluid；For spleen Lung Qi deficiency, eat less and tired, false asthma cough, insufficiency of vital energy and blood, sallow complexion, palpitation.

The root of large-flowered skullcap, is the dry root of the labiate root of large-flowered skullcap, bitter, cold, return lung, courage, spleen, large intestine, small intestinl channel；Heat-clearing is dry It is wet, purging intense heat and detonicating, hemostasis, antiabortive；For damp-warm syndrome, summer-heat and damp, evil of vomitting uncomfortable in chest, damp and hot feeling of fullness, rush down dysentery, jaundice, cough with lung heat, hyperpyrexia Polydipsia, blood-head tells nosebleed, carbuncle sore tumefacting virus, fetal irritability.

Os Draconis, are Skeletal Fossils of the ancient times mammal like an elephant class, rhinoceros class, Hippocampal cortex etc., sweet, puckery, flat, are returned The heart, liver, kidney, large intestine channel；Calming the liver, tranquillizing the mind by relieving convulsion is astringent or styptic treatment for spontaneous sweating；For frightened epilepsy it is demented, palpitation is forgetful, insomnia and dreamful sleep, spontaneous perspiration steal Sweat, seminal emission stranguria with turbid discharge, hematemesis is had blood in stool, uterine bleeding under band, rush down dysentery prolapse of the anus, ulcer do not close up long.

Raw oyster, is the shell of Ostreidae animal Crassostrea rivularis, oyster long or ostrea talienwhanensis Crosse etc., and taste is salty, be slightly cold, and is returned Liver, courage, kidney channel；Beneficial the moon checking exuberance of yang, softening and resolving hard mass is astringent or styptic treatment for spontaneous sweating；For insomnia of palpitating with fear, dizziness and tinnitus, scrofula subcutaneous nodule, abdominal lump of mass in abdomen, from Sweat night sweat, seminal emission collapse band, stomachache pantothenic acid.

Cassia twig, is the dry twig of canella Chinese cassia tree, taste is pungent, sweet, temperature, the thoughts of returning home, lung, bladder warp；Sweating expelling pathogenic factors from muscles and skin, Wen Tong Passages through which vital energy circulates, helps yang transforming qi, flat gas that spins；For anemofrigid cold, coldness and pain in the epigastrium, thromboembolism Amenorrhea, joint pain, phlegm and retained fluid oedema, palpitaition Run quickly globefish.

Poria cocos, is the dry sclerotia of On Polyporaceae Poria cocos, sweet, light, flat, the thoughts of returning home, lung, spleen, kidney channel；Li Shui oozes Wet, invigorating the spleen calming heart；For oedema oliguria, phlegm retention anti-dazzle nervous, spleen eating less, loose stool diarrhea, confused and worried, insomnia of palpitating with fear.

Prepared RHIZOMA PINELLIZE without adju-vant, is the dry tuber of aroid prepared RHIZOMA PINELLIZE without adju-vant, taste is pungent, temperature, returns spleen, stomach, lung channel；Eliminating dampness and eliminating phlegm, Stopping nausea and vomiting by lowering the adverse flow of QI, dissolving lump and resolving mass；For the cold phlegm of damp phlegm, cough and phlegm, phlegm retention anti-dazzle nervous, anemophlegmatic vertigo, coma due to blocking of the respiratory system headache, vomiting gastric disorder causing nausea, Chest gastral cavity ruffian, globus hysteriocus.

Radix bupleuri, is the dry root of umbelliferae bupleurum or radix bupleuri scorzoneraefolii, taste is pungent, hardship, is slightly cold, Return liver, courage, lung channel；Dredge Dissipate bring down a fever, soothing liver-qi stagnation, rise lift yang-energy；For cold, fever, fevers and chills alternate, distending pain in the chest and hypochondrium, irregular menstruation, the prolapse of uterus, de- Anus.

Rheum officinale, is the dry root and rhizome of polygonum rheum palmatum, Rheum tanguticum Maxim or Rheum officinale, bitter, cold, is returned Spleen, stomach, large intestine, liver, pericardium channel；Heat and toxic materials clearing away, clearing heat-fire, removing pattogenic heat from the blood and toxic material from the body, dispelling stasis of blood and stimulating the menstrual flow, removing dampness through diuresis and removing jaundice；For damp and hot product Stagnant constipation, blood-head tells nosebleed, hot eyes pharynx swelling, carbuncle swells furunculosis, abdominalgia with intestinal abscess, blood stasis closed, postpartum stasis, traumatic injury, hygropyretic dysentery The red urine of disease, jaundice, stranguria oedema.

Fry two ugly, be the dry mature fruit that convolvulaceous plant pharbitis nilChoisy or garden leaf are led a cow, it is bitter, cold, return lung, kidney, Large intestine channel；Water pouring defaecation, dissolving phlegm are washed drink, desinsection and attack product.Gathered for oedema turgor, constipation and anuresis, phlegm and retained fluid, the circulation of vital energy in the wrong direction is breathed with cough, worm Product stomachache.

Moutan bark, is the dry root skin of ranunculaceae peony, taste is pungent, is slightly cold, the thoughts of returning home, liver and kidney channel；Clearing heat and cooling blood, it is living Blood stagnation resolvation；Enter ying blood, febrile virulent maculae, hematemesis and epistaxis, night fever abating at dawn, lossless hectic fever due to yin, closed dysmenorrhea, the pain of injury caused by falling and tumbling, carbuncle for heat Swollen sore.

Gypsum, is Sulfates mineral anhydrite race gypsum, in sweet, pungent, Great Cold, is attached to the lung and stomach meridians；Clearing heat-fire, remove It is tired to quench the thirst；For fever caused by exogenous pathogenic factors, hyperpyrexia polydipsia, dyspnea and cough due to lung-heat, the high Sheng of stomach fire, headache, toothache.

Gypsum rubrum, is calcium sulfate or calcium carbonate mineral, taste is pungent, salty, cold, the thoughts of returning home, stomach, kidney channel；Clearing heat-fire, sharp key, disappears It is swollen；For pyreticosis polydipsia, erysipelas is scalded.

Rhizoma zingiberis, is the dry rhizome of zingiber, taste is pungent, heat, returns spleen, stomach, kidney, the heart, lung channel；Warm cold dispelling, Hui Yang Promote blood circulation, Wenfei of drink；Breathed with cough for coldness and pain in the epigastrium, vomiting is had loose bowels, cold, the cold drink of the cold arteries and veins of limb.

Great Bei, is the dry bulb of liliaceous plant fritillary bulb, bitter, it is sweet, be slightly cold, return lung, the heart channel of Hang-Shaoyin；Clearing heat and moistening lung, change Phlegm and relieving cough；Coughed for lung-heat type cough, the few phlegm of dry cough, deficiency of Yin labor, cough sputum streaked with blood.

Radix scrophulariae, is the dry root of goatweed radix scrophulariae, sweet, bitter, salty, be slightly cold, return lung, stomach, kidney channel；Clearing heat and cooling blood, Nourishing Yin and falling fire, detoxicating and resolving a mass；For heat enter ying blood, febrile virulent maculae, consumption of YIN caused by febrile disease, the deep red polydipsia of tongue, Tianjin hinder constipation, hectic fever due to yin labor is coughed, Hot eyes pharyngalgia, diphtheria, carbuncle sore tumefacting virus.

Licorice, is the dry root and rhizome of glycyrrhizic legume, swollen fruit Radix or glycyrrhiza glabra, sweet, flat, the thoughts of returning home, Lung, spleen, stomach；Invigorate the spleen and benefit qi, clearing heat and detoxicating, expelling phlegm and arresting coughing, relieving spasm to stop pain, coordinating the drug actions of a prescription；It is weary for weakness of the spleen and the stomach, burnout Power, palpitation, coughing with a lot of sputum, gastral cavity abdomen, four limbs convulsion urgency pain, carbuncle sore tumefacting virus, alleviate drug toxicity, strong.

Date, is the dry mature fruit of Rhamnaceae plant jujube, sweet, temperature, is returned, spleen, stomach, the heart channel of Hang-Shaoyin；Tonifying middle-Jiao and Qi, blood-nourishing Calm the nerves；For spleen eating less, weak loose stool, hysteria of womam.

Effective compatibility of said medicine, mutually collaboration, mutually support, with strengthening spleen, tonifying kidney, dissipating phlegm and removing blood stasis, strengthening vital QI to eliminate pathogenic factors it Effect, is conducive to improving body's immunity, is to prevent the innovation on glioma postoperative recurrence medicine, and through clinical test and Using, very satisfied Advantageous Effects are achieved, relevant information is as follows：

Chinese medicine composition decocting liquid is to C6（Brain glioblastoma cell）Proliferative effect research experiment data

1. experiment material

1.1 experiment cells

C6（Brain glioblastoma cell）, purchased from Shanghai Inst. of Life Science, CAS cell resource center, use RIMP1640 culture mediums and hyclone, are placed in 37 DEG C and 5% CO₂Concentration incubator culture.

1.2 Experimental agents

Chinese medicine composition of the present invention.

1.3 experiment reagents

RIMP1640（GIBCO companies）；NBCS（The Hangzhou four seasons clear biological engineering material Co., Ltd, lot number： 150928）；0.25% trypsase（GIBCO companies）；Dimethyl sulfoxide（SIGMA companies）；EDTA（SIGMA companies）；Cell is bred And citotoxicity detection kit（Shanghai Bo Gu bio tech ltd, lot number：9368593）；

1.4 laboratory apparatus

The double one side clean bench of SJ-CJ-2FDQ types（Suzhou purifying equipment Co., Ltd of Soviet Union）；ESW-8CQ types CO2 is cultivated Case（Thermo Fisher Scientific, the U.S.）；Inverted phase contrast microscope（Leica, Germany）；Accurate pipettor （Eppendof, Germany）；Low speed centrifuge（Anhui Zhong Kezhongjia scientific instrument Co., Ltd）；Full-automatic high-pressure autoclave （GR60DR, Xiamen causes micro-）；Digital display three uses constant temperature water tank（Suzhou Weir experimental article Co., Ltd）；The ultrapure water systems of Milli-Q System（Merck Mi Libo）；Electronic balance（XS205, plum Teller-support benefit, Switzerland）；Cell counting count board；96 well culture plates； 0.2 μm of worry is first-class.

2. experimental technique

1. C6 cells are cultivated in 37 DEG C and 5% CO2 incubators with RIMP1640,10% hyclone, when cell growth to logarithm During the phase, discard nutrient solution and washed 3 times with PBS, add trypsase and EDTA to be digested 3 minutes in 37 DEG C, be subsequently adding culture medium, Attached cell is gently blown and beaten with pipettor to suspending completely, centrifuge tube is transferred to and is centrifuged 10 minutes in 1000rpm, adjust cell density It is 2 × 10⁴Individual/ml.

2. cell is added in 96 orifice plates（Per hole about 2 × 10⁴Individual cell）, it is placed in 37 DEG C and 5% CO2 incubators and cultivates 24 hours.

3. according to cell growth status, the appropriate concentration of example 1 decocting liquid is added, when continuation is incubated appropriate in incubator Between.

4. 5 × MTT is diluted to 1 × MTT.

5. 50 μ 1 × MTT of l are added per hole, is incubated 4 hours at 37 DEG C, MTT is reduced to first month and praise.

6. supernatant is then suctioned out, adds 200 μ l DMSO the first moon is praised abundant dissolving per hole, shaken up with plate shaker. ELIASA detects the optical density per hole at 570nm wavelength.

7. the OD values of each instrument connection are subtracted into background OD values or blank medicine hole OD values, the OD values of each repeating hole are taken the mean ± standard deviation；With cell survival rate（T/C%）To represent inhibition of the medicine to cell, T is the OD values of dosing cell, and C is right The OD values of photo cell；Cell survival rate %=（Dosing cell OD values/compared with control cells OD values）× 100%, every group of experiment repeats three It is secondary.

3. experimental result

Statistical result showed after the completion of MTT experiment, compares with blank control group, thin to C6 when drug dose reaches 10mg/ml Born of the same parents' Proliferation Ability is variant（P ﹤ 0.01）, there is pole significant difference when dosage is more than 15mg/ml（P ﹤ 0.001）；Result is shown in Table 1.

Table 1 is in 570nm wavelength OD values and its survival rate

Blank

Positive control

5mg/ml

10mg/ml

15mg/ml

20mg/ml

OD values（Mean±SD）

0.466±0.0045

0.255±0.0056

0.412±0.0048

0.332±0.0041

0.232±0.0039

0.189±0.0043

Cell survival rate（%）

100%

54.7%

88.4%

71.2% *

49.8% * *

40.6% * *

Background

0.007

0.006

0.008

0.007

0.006

0.007

Note：Compare with control group, * P ﹤ 0.01;* P ﹤ 0.001.

4. experiment conclusion

Chinese medicine composition of the invention can suppress C6（Brain glioblastoma cell）Cell is bred, and reduces the growing number of C6 cells, And the inhibitory action is presented drug dose dependence, and positive control drug Temozolomide has equal curative effect, but without Temozolomide Toxic and side effect, illustrate that Chinese medicine composition of the invention inhibits the propagation of glioma cell well, prevention glioma Postoperative recurrence has advantage.

According to clinical practical application, getting well for its curative effect did not expected that relevant clinical data was as follows.

1st, physical data：Accept Patients with gliomas 120, wherein male 73 for medical treatment altogether, women 47, age 17-72 Sui, Average age 38 years old.By 4 grades of criteria for classifications of WHO gliomas, number of cases at different levels such as following table.

	1 grade（WHO）	2 grades（WHO）	3 grades（WHO）	4 grades（WHO）
					Number of cases	5	47	23	45

Therapeutic scheme：Chinese medicine composition of the present invention is decocted in water for oral dose, twice daily, morning and evening sub-service, each 200-300mL, one The moon is a course for the treatment of, takes 2-3 course for the treatment of statistics curative effect.

2nd, curative effect determinate standard：

Clinical cure：Clinical symptom disappearance, imageological examination tumor disappearance does not recur in 5 years；

It is clinical effective：Clinical symptoms are most of to disappear, imageological examination tumor regression；

It is clinical effective：Clinical symptoms are relieved, and imageological examination tumour is without significant change；

It is clinical invalid：Clinical symptoms are unchanged, the increase of imageological examination tumour.

3rd, statistical procedures

Clinical cure 43 in 120 patients, effective 52, effective 17, invalid 8, total effective rate 93.3%, determined curative effect Significantly, there are not other discomforts in patient in therapeutic process.

By the above results as can be seen that Chinese medicine composition of the present invention has the work(of strengthening spleen, tonifying kidney, dissipating phlegm and removing blood stasis, strengthening vital QI to eliminate pathogenic factors Effect, is conducive to improving body's immunity, controls tumor proliferation, and attenuation synergistic, prevention recurrence extends life cycle, is prevention brain Innovation on After gliomas operation recurrence medicine, compared with prior art, with following Advantageous advantage：

（1）The effect of this formula has strengthening spleen, tonifying kidney, dissipating phlegm and removing blood stasis, strengthening vital QI to eliminate pathogenic factors, collectively promotes the vigorous of human righteousness, favorably In raising body's immunity；

（2）Chinese medicine in the present invention is natural botanical source, and security is good, there is actual economic worth and clinical value, is With the innovation on traditional Chinese medicine prevention glioma postoperative recurrence；

（3）Low cost of the present invention, material medicine enriches, prepared by easily production；

（4）Easy to use, effect is good, and curative effect is up to more than 93%, strong beneficial to body with very strong actual application value Health, beneficial to industrialized production and commercial popularization, obtains business success, there is good economic and social benefit.

Claims

1. it is a kind of prevent glioma postoperative recurrence Chinese medicine composition, it is characterised in that by the raw material of following parts by weight meter Medicine is made：Radix Codonopsis 1-8 parts, root of large-flowered skullcap 1-5 parts, Os Draconis 1-5 parts, raw oyster 1-5 parts, cassia twig 1-15 parts, Poria cocos 1-10 parts, clearly Tuber of pinellia 1-5 parts, radix bupleuri 1-8 parts, rheum officinale 1-15 parts, fry two ugly 1-5 parts, moutan bark 1-7 parts, gypsum 1-15 parts, gypsum rubrum 1-5 Part, rhizoma zingiberis 1-7 parts, great Bei 1-7 parts, radix scrophulariae 1-10 parts, licorice 1-8 parts and date 1-5 parts, wherein, first will be except Os Draconis, life Other 16 taste material medicines of oyster are mixed, and add the water of other 7-10 times of 16 taste material medicine weight, soak 30- 60min；Os Draconis, raw oyster are mixed, plus Os Draconis, the water of 7-10 times of raw oyster weight, 30-60min is soaked, decoct 30-40min is boiled, in other the 16 taste material medicines poured into after immersion 30-60min, then 30-40min is decocted, filtered, obtain first Secondary filtrate；The dregs of a decoction add the water of 7-10 times of material medicine weight, decoct 30-40min, filter to obtain second filtrate, merge two Secondary filtrate, is mixed into Chinese medicine liquid.

2. it is according to claim 1 prevention glioma postoperative recurrence Chinese medicine composition, it is characterised in that by following heavy The material medicine for measuring number meter is made：7 parts of Radix Codonopsis, 4 parts of the root of large-flowered skullcap, 4 parts of Os Draconis, 4 parts of raw oyster, 8 parts of cassia twig, 8 parts of Poria cocos, clearly 4 parts of the tuber of pinellia, 6 parts of radix bupleuri, 9 parts of rheum officinale, ugly 4 parts of stir-fry two, 6 parts of moutan bark, 9 parts of gypsum, 4 parts of gypsum rubrum, 6 parts of rhizoma zingiberis, great Bei 5 3 parts of part, 8 parts of radix scrophulariae, 6 parts of licorice and date, preparation method is with claim 1.

3. it is according to claim 1 prevention glioma postoperative recurrence Chinese medicine composition, it is characterised in that by following heavy The material medicine for measuring number meter is made：5 parts of Radix Codonopsis, 6 parts of the root of large-flowered skullcap, 3 parts of Os Draconis, 3 parts of raw oyster, 5 parts of cassia twig, 3 parts of Poria cocos, clearly 6 parts of the tuber of pinellia, 4 parts of radix bupleuri, 5 parts of rheum officinale, ugly 6 parts of stir-fry two, 4 parts of moutan bark, 5 parts of gypsum, 6 parts of gypsum rubrum, 3 parts of rhizoma zingiberis, great Bei 3 6 parts of part, 6 parts of radix scrophulariae, 4 parts of licorice and date, preparation method is with claim 1.

4. it is according to claim 1 prevention glioma postoperative recurrence Chinese medicine composition, it is characterised in that by following heavy The material medicine for measuring number meter is made：3 parts of Radix Codonopsis, 4 parts of the root of large-flowered skullcap, 2 parts of Os Draconis, 2 parts of raw oyster, 4 parts of cassia twig, 4 parts of Poria cocos, clearly 2 parts of the tuber of pinellia, 3 parts of radix bupleuri, 4 parts of rheum officinale, ugly 3 parts of stir-fry two, 5 parts of moutan bark, 7 parts of gypsum, 3 parts of gypsum rubrum, 5 parts of rhizoma zingiberis, great Bei 3 5 parts of part, 2 parts of radix scrophulariae, 8 parts of licorice and date, preparation method is with claim 1.

5. it is according to claim 1 prevention glioma postoperative recurrence Chinese medicine composition, it is characterised in that by following heavy The material medicine for measuring number meter is made：2 parts of Radix Codonopsis, 3 parts of the root of large-flowered skullcap, 3 parts of Os Draconis, 3 parts of raw oyster, 2 parts of cassia twig, 2 parts of Poria cocos, clearly 3 parts of the tuber of pinellia, 3 parts of radix bupleuri, 2 parts of rheum officinale, ugly 2 parts of stir-fry two, 2 parts of moutan bark, 6 parts of gypsum, 2 parts of gypsum rubrum, 3 parts of rhizoma zingiberis, great Bei 2 Part, 3 parts of radix scrophulariae, 3 parts of licorice, 5 parts of date, preparation method is with claim 1.

6. it is according to claim 1 prevention glioma postoperative recurrence Chinese medicine composition, it is characterised in that by following heavy The material medicine of gauge is made：Radix Codonopsis 100g, root of large-flowered skullcap 100g, Os Draconis 300g, raw oyster 300g, cassia twig 100g, Poria cocos 120g, Prepared RHIZOMA PINELLIZE without adju-vant 100g, radix bupleuri 100g, rheum officinale 100g, fry two ugly 60g, moutan bark 100g, gypsum 300g, gypsum rubrum 100g, rhizoma zingiberis 100g, great Bei 100g, radix scrophulariae 300g, licorice 60g and date 200g, preparation method is with claim 1.

7. it is according to claim 1 prevention glioma postoperative recurrence Chinese medicine composition, it is characterised in that by following heavy The material medicine of gauge is made：Radix Codonopsis 200g, root of large-flowered skullcap 200g, Os Draconis 100g, raw oyster 100g, cassia twig 200g, Poria cocos 150g, Prepared RHIZOMA PINELLIZE without adju-vant 200g, radix bupleuri 200g, rheum officinale 200g, fry two ugly 100g, moutan bark 200g, gypsum 100g, gypsum rubrum 200g, rhizoma zingiberis 200g, great Bei 200g, radix scrophulariae 100g, licorice 100g and date 100g, preparation method is with claim 1.

8. it is according to claim 1 prevention glioma postoperative recurrence Chinese medicine composition, it is characterised in that by following heavy The material medicine of gauge is made：Radix Codonopsis 50g, root of large-flowered skullcap 50g, Os Draconis 150g, raw oyster 150g, cassia twig 50g, Poria cocos 50g, clear half Summer 60g, radix bupleuri 60g, rheum officinale 60g, fry two ugly 30g, moutan bark 50g, gypsum 150g, gypsum rubrum 50g, rhizoma zingiberis 50g, great Bei 60g, radix scrophulariae 150g, licorice 100g and date 80g, preparation method is with claim 1.

9. it is according to claim 1 prevention glioma postoperative recurrence Chinese medicine composition, it is characterised in that by following heavy The material medicine of gauge is made：Radix Codonopsis 30g, root of large-flowered skullcap 30g, Os Draconis 80g, raw oyster 80g, cassia twig 30g, Poria cocos 40g, prepared RHIZOMA PINELLIZE without adju-vant 40g, radix bupleuri 20g, rheum officinale 30g, fry two ugly 20g, moutan bark 30g, gypsum 50g, gypsum rubrum 40g, rhizoma zingiberis 30g, great Bei 30g, Radix scrophulariae 20g, licorice 30g and date 30g, preparation method is with claim 1.