CN106820160B - Microelement selenium dietary supplement with function of relieving salmonella induced enteritis - Google Patents

Microelement selenium dietary supplement with function of relieving salmonella induced enteritis Download PDF

Info

Publication number
CN106820160B
CN106820160B CN201710066209.1A CN201710066209A CN106820160B CN 106820160 B CN106820160 B CN 106820160B CN 201710066209 A CN201710066209 A CN 201710066209A CN 106820160 B CN106820160 B CN 106820160B
Authority
CN
China
Prior art keywords
salmonella
dietary supplement
selenium
feed
relieving
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201710066209.1A
Other languages
Chinese (zh)
Other versions
CN106820160A (en
Inventor
陈卫
田丰伟
翟齐啸
李鹏
赵建新
张灏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangnan University
Original Assignee
Jiangnan University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangnan University filed Critical Jiangnan University
Priority to CN201710066209.1A priority Critical patent/CN106820160B/en
Publication of CN106820160A publication Critical patent/CN106820160A/en
Application granted granted Critical
Publication of CN106820160B publication Critical patent/CN106820160B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/1322—Inorganic compounds; Minerals, including organic salts thereof, oligo-elements; Amino-acids, peptides, protein-hydrolysates or derivatives; Nucleic acids or derivatives; Yeast extract or autolysate; Vitamins; Antibiotics; Bacteriocins
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D13/00—Finished or partly finished bakery products
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
    • A21D2/02—Treatment of flour or dough by adding materials thereto before or during baking by adding inorganic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/04—Sulfur, selenium or tellurium; Compounds thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention discloses a trace element selenium dietary supplement with a function of relieving salmonella induced enteritis, and belongs to the technical field of foods. The main beneficial component of the microelement selenium dietary supplement is sodium selenite, which can inhibit infection of salmonella on immune organs of mice and obviously improve the oxidation resistance index of mouse organisms. The microelement selenium dietary supplement is used for preparing fermented milk, wheat products and pharmaceutical compositions with the function of relieving salmonella induced enteritis, and has very wide application prospect.

Description

Microelement selenium dietary supplement with function of relieving salmonella induced enteritis
Technical Field
The invention relates to a trace element selenium dietary supplement with a function of relieving salmonella induced enteritis, belonging to the technical field of foods.
Background
Salmonella typhimurium is a pathogenic bacterium that infects many mammalian hosts, causing intestinal disease and diarrhea and even death. It belongs to a pathogenic bacterium which is not host-specific but invasive, and it is reported that 40 to 80% of food poisoning accidents in developed countries such as Japan and the United states are caused by Salmonella infection. In 1981, more than 2000 species of salmonella were published by the world health organization, with 53-80% of the pediatric salmonella infections reported in the literature. The characteristics of the salmonella typhimurium are as follows: aerobic, spore-free, flagellated gram-negative bacteria. Has strong ability of living adapting to the outside world and is widely distributed in the nature. Common sources of infection are: carriers of temporarily asymptomatic salmonella, internal organs of poultry and other animals, contaminated healthy livestock breeding, eggs and egg products, etc. The digestive tract is the primary route of infection of the body by salmonella. Ingestion of food and water contaminated with salmonella can cause the onset of infection. After entering the intestinal tract, salmonella causes disease by endotoxin. Part of the salmonella can be colonized in the intestinal tract, and most of the salmonella is excreted out of the body through the feces. The latent period of salmonella is indefinite, and the most common clinical types are enteritis such as aversion to cold, fever, vomiting, diarrhea, abdominal pain, water sample of stool and the like. The current effective approach to salmonella infection is to use antibiotics, such as oxytetracycline, chloramphenicol, and the like, with symptomatic treatment. Furans and sulfonamides also have certain effects.
With the widespread use of antibiotics, the resistance of salmonella, particularly salmonella typhimurium, to antibiotics has also increased substantially. Although only a small proportion of salmonella will have resistance to antibiotics (or spontaneous mutation), after extensive use of antibiotics, this small proportion of bacteria multiply to become dominant flora and cause enteritis in the body. Therefore, there is a need to find a new method for better preventing or controlling Salmonella infection in the body without side effects.
Disclosure of Invention
In order to solve the problems, the invention provides a trace element selenium dietary supplement for relieving infection of salmonella on organisms and intestinal tract infection induced by salmonella, which takes sodium selenite as an effective component or a main effective component.
The first purpose of the invention is to provide a dietary supplement capable of relieving infection of salmonella on the body or intestinal tract-induced symptoms of salmonella, wherein the dietary supplement takes sodium selenite as a main effective component.
In one embodiment, the dietary supplement is a feed, beverage, wheat flour, wheat product, or the like.
In one embodiment, the feed further comprises a carrier acceptable in the feed.
In one embodiment, the feed is a pellet-type feed.
In one embodiment, the feed is a mouse feed.
In one embodiment, the preparation of the feed comprises: dissolving sodium selenite in deionized water, mixing with feed, and granulating; wherein the selenium content is 0.15-2.00 mg/kg feed.
In one embodiment, the selenium content of the feed is 0.40-1.00 mg/kg feed.
In one embodiment, the beverage is a milk-containing beverage, such as fermented milk.
In one embodiment, the wheat product comprises bread or the like.
The second purpose of the invention is to provide a medicine or a medicine composition capable of relieving infection of salmonella on the body or intestinal tract-induced symptoms of salmonella, wherein the medicine or the medicine composition takes sodium selenite as a main effective component.
In one embodiment, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier.
In one embodiment, the sodium selenite in the pharmaceutical composition is sodium selenite powder obtained by freeze-drying.
In one embodiment, the drug or pharmaceutical composition may be formulated in several dosage forms containing some excipients commonly used in the pharmaceutical arts; for example, oral formulations (e.g., tablets, capsules, solutions or suspensions); injectable formulations (e.g., injectable solutions or suspensions, or injectable dry powders, which are immediately ready for use by addition of water for injection prior to injection); topical formulations (e.g. ointments or solutions).
In one embodiment, the pharmaceutical composition is a capsule or tablet.
In one embodiment, the carrier for the pharmaceutical composition of the invention is of the usual type available in the pharmaceutical field, including: binders, lubricants, disintegrants, solubilizing agents, diluents, stabilizers, suspending agents, non-coloring agents, flavoring agents, etc. for oral preparations; preservatives, solubilizers, stabilizers and the like for injectable preparations; bases for topical formulations, diluents, lubricants, preservatives, and the like.
In one embodiment, the pharmaceutical composition is placed with a label; the label is recorded with the selenium intake of 200-400 mug/d.
The sodium selenite of the invention meets the edible requirements, is in a crystalline powder form, is soluble in water and is insoluble in ethanol. Its melting point is 350 deg.C, purity is greater than 99.99%, molecular weight is 119.9634, it is stable in air, sensitive to humidity and easy to crystallize.
The trace element selenium dietary supplement or the medicament or the pharmaceutical composition of the invention has the following beneficial properties:
1. the infection of salmonella on body tissues can be inhibited;
2. can improve oxidation resistance of organism.
The invention shows that when the addition amount of the trace element selenium dietary supplement is 200-400 mu g/d, the infection of salmonella on different organs can be effectively inhibited, the immunity of intestinal tracts is improved, and the oxidative stress damage to organisms caused by the infection of the salmonella is reduced.
Drawings
Fig. 1 is a flow of a feed preparation method.
Detailed Description
Example 1: preparation of mouse feed
The preparation method of the feed is shown in figure 1.
Dissolving sodium selenite in deionized water, mixing with feed, and granulating. 0.15, 0.40, 1.00 and 2.00mg of sodium selenite are respectively weighed, dissolved in 200mL of deionized water, poured into 1kg of feed raw materials, mixed and granulated. The feeds with the selenium contents of 0.15, 0.40, 1.00 and 2.00mg/kg are formed and are respectively named as a control group, a selenium-rich group, a high-selenium group and a very-high-selenium group. They correspond to human intakes of 50, 200, 400 and 800ug/d, respectively. In the practice of the invention, the ingredients of the feed are shown in tables 1 and 2 below:
TABLE 1 basal feed composition and content
Figure BDA0001220910860000031
TABLE 2 basal feed composition and nutrient content
Figure BDA0001220910860000032
Example 2: effect of a microelement selenium dietary supplement on Salmonella infection of mouse tissues
40 four week-old SPF-rated mice were randomly divided into 4 groups of 10 mice each. The group is a control group, a selenium-rich group, a high-selenium group and a very high-selenium group. Each group was fed with feeds containing 0.15, 0.40, 1.00 and 2.00ppm selenium respectively. Mice were fed water ad libitum for 2 months. After the two months, all mice were fasted for 4h with no water, and mice were returned to free diet after oral gavage with 20mg of streptomycin. After 20h streptomycin treatment, fasting was again performed for 4h, and 5X 10 diluted with PBS was gavaged orally7CFU salmonella typhimurium SL 1344. All mice were sacrificed after 48h for dissection, and spleen and mesenteric lymph node (mLN) were washed with physiological saline and homogenized in physiological saline containing 1% Triton 100, and the homogenate was spread on Macconk plate medium containing 0.5% streptomycin to count the number of Salmonella therein.
TABLE 3 infection degree of Salmonella on immune organs
Figure BDA0001220910860000041
Note: p <0.05 compared to control.
After the salmonella invades the intestinal tract of the body, the salmonella can invade immune organs around the digestive tract, and oxidative stress damage is caused to the immune organs. The data in table 3 show that the amount of salmonella in the selenium-rich group and the selenium-rich group is significantly less than that in the control group, both in spleen and mesenteric lymph node, which indicates that the invention can effectively inhibit the infection of salmonella to the immune organs of the organism in the selenium-rich group and the selenium-rich group, thereby relieving the damage of the organism caused by enteritis induced by salmonella.
Example 3: effect of a microelement selenium dietary supplement on the antioxidant Capacity of mice
Following the mouse experiments described in example 1, after completion of the experiments, blood was taken from the orbit to kill the mice, and the livers of the mice were washed with pre-cooled physiological saline and homogenized. Measuring antioxidant indexes such as SOD (superoxide dismutase), MDA (malondialdehyde) and T-AOC (total antioxidant capacity) in the liver. The test results are given in the table below.
TABLE 4 mouse liver Oxidation resistance assay
Figure BDA0001220910860000042
Note: indicates that the group has significant difference compared with the control group, p is < 0.05; indicates that the group had a very significant difference compared to the control group, p < 0.01.
After the salmonella invades the body, the salmonella secretes endotoxin through self metabolism, and the oxidative stress effect of body cells is increased. It induced enteritis can also increase the concentration of active oxygen in the body, causing greater oxidative stress damage to the body. SOD (superoxide dismutase) is an active substance in organisms, the main functions of the SOD are to eliminate harmful substances such as free radicals generated by the organisms in the metabolic process and repair cells damaged by oxidative stress effect, T-AOC (total antioxidant capacity) is a comprehensive index for evaluating the antioxidant capacity of the organisms, and the higher the antioxidant capacity of organism tissues, the larger the value of the T-AOC. MDA (malondialdehyde) is a marked product of peroxidation of free radicals and lipid in organisms, and is an index of oxidative stress injury in organisms.
As can be seen from the results in table 4, the trace element selenium dietary supplement of the present invention can effectively improve the antioxidant ability of mice. The SOD in the liver of the selenium-enriched mice is obviously higher than that in the control group, and although the high-selenium group has no obvious difference compared with the control group, the SOD is slightly increased. The two groups are obviously higher than the control group in the level of T-AOC, which shows that the total antioxidant capacity of the two groups is higher than that of the control group, and the antioxidant capacity of the organism is enhanced. As can be seen from the MDA value, the MDA value of the high-selenium group is obviously lower than that of the control group, and although the selenium-rich group and the control group have no obvious difference, the MDA concentration is also reduced, which indicates that the two groups of mice have no obvious influence on the salmonella oxidative stress effect. The very high selenium group showed the opposite trend. The above data fully demonstrate that the microelement selenium dietary supplement of the present invention can indeed alleviate the oxidative stress effect on the body belt caused by enteritis induced by salmonella infection in the selenium-rich group and the high-selenium group.
Application example 1: fermented milk containing microelement selenium dietary additive
Adding sugar into fresh milk to dissolve, adding a certain amount of sodium selenite to ensure that the concentration of the sodium selenite is 200 mug/kg, homogenizing at 60 ℃ and 20MPa, then sterilizing at 95 ℃ for 5min, cooling to 35 ℃, adding a commercial dry powder starter, namely lactobacillus bulgaricus and a commercial dry powder starter, namely streptococcus thermophilus, wherein the inoculation amount of the mixed bacteria is 0.03 percent of the weight of the fresh milk, uniformly mixing, performing heat preservation fermentation at 40 ℃, curdling, and refrigerating at 4 ℃ for 24 hours to obtain the selenium-enriched fermented milk.
Application example 2: bread for preparing dietary additive containing trace element selenium
Dissolving 10g of white granulated sugar and 200 mu g of sodium selenite in 300ml of warm water (41-45 ℃), spraying dry yeast on the water, stirring, and standing for 10-15 min. Then pouring water into 1kg of flour, mixing, kneading, and standing at room temperature for 60 min. Flattening dough with fist to eliminate air bubble, dividing dough into equal parts, fermenting at room temperature for 60-120 min. Coating a little butter on the surface of the dough, and baking the dough in an oven at 220 ℃ for 30-35 min. And cooling to room temperature to obtain the selenium-rich bread.
Application example 3: capsule product for preparing dietary supplement containing trace element selenium
Weighing 200 mu g of sodium selenite, adding the sodium selenite into a sodium alginate solution accounting for 3 percent by weight, fully stirring and dissolving, then forming colloidal particles in a calcium chloride solution accounting for 2 percent by weight of the mixed solution, standing and solidifying for 30min, filtering and collecting the colloidal particles, freezing and drying the collected colloidal particles for 48h to obtain powder containing the sodium selenite, and filling the powder into medicinal capsules sold in the market at present to obtain the capsule product.
Application example 4: tablet for preparing dietary supplement containing trace element selenium
25.7 parts by weight of sodium selenite powder prepared by a freeze drying method, 55.0 parts by weight of starch, 4.5 parts by weight of cellulose derivative, 12.0 parts by weight of sodium carboxymethyl starch, 0.8 parts by weight of talcum powder, 1.0 part by weight of cane sugar and 1.0 part by weight of water are respectively weighed, fully and uniformly mixed, prepared into wet granules by a conventional method, and then tableted by a tablet press produced by pharmaceutical machinery factories in the south of China, for example, and then dried in a small-sized pharmaceutical drying agent produced by Yikang traditional Chinese medicine machinery Limited company in Qingzhou city, and then packaged to obtain the tablet of the invention.
Application example 5: mouse granulated feed for preparing dietary supplement containing trace element selenium
Adding 0.4-1 mg of sodium selenite into 1kg of powdery premixed feed, fully and uniformly mixing, and granulating by using a small-sized granulated feed machine at the granulating temperature of 65 ℃ to obtain the selenium-rich granulated feed, wherein the selenium content of the obtained granulated feed is 0.4-1 mg/kg.
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.

Claims (3)

1. The application of sodium selenite in preparing a dietary supplement capable of relieving infection of salmonella on the body is characterized in that the dietary supplement takes sodium selenite as a main effective component; dissolving sodium selenite in deionized water, mixing with feed, and granulating; wherein the selenium content is 0.40-1.00 mg/kg feed.
2. The use according to claim 1, wherein the feed further comprises a carrier acceptable in feed.
3. Use according to claim 1, wherein the feed is a pellet-type feed.
CN201710066209.1A 2017-02-06 2017-02-06 Microelement selenium dietary supplement with function of relieving salmonella induced enteritis Active CN106820160B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710066209.1A CN106820160B (en) 2017-02-06 2017-02-06 Microelement selenium dietary supplement with function of relieving salmonella induced enteritis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710066209.1A CN106820160B (en) 2017-02-06 2017-02-06 Microelement selenium dietary supplement with function of relieving salmonella induced enteritis

Publications (2)

Publication Number Publication Date
CN106820160A CN106820160A (en) 2017-06-13
CN106820160B true CN106820160B (en) 2021-03-30

Family

ID=59122930

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710066209.1A Active CN106820160B (en) 2017-02-06 2017-02-06 Microelement selenium dietary supplement with function of relieving salmonella induced enteritis

Country Status (1)

Country Link
CN (1) CN106820160B (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102573523A (en) * 2009-08-11 2012-07-11 金娟辰 Health supplement food, feed, and pharmaceutical composition comprising chia seed and maca, and manufacturing method thereof
CN105248932A (en) * 2015-08-25 2016-01-20 四川农业大学 Trace element premix capable of reducing propagation and invasion of Salmonella Enteritidis phage type 4 in laying hen and preparation method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102573523A (en) * 2009-08-11 2012-07-11 金娟辰 Health supplement food, feed, and pharmaceutical composition comprising chia seed and maca, and manufacturing method thereof
CN105248932A (en) * 2015-08-25 2016-01-20 四川农业大学 Trace element premix capable of reducing propagation and invasion of Salmonella Enteritidis phage type 4 in laying hen and preparation method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
亚硒酸钠等五种化合物对黄曲霉毒素;阮萃才等;《广西医学院学报》;19920331;第9卷(第3期);全文 *

Also Published As

Publication number Publication date
CN106820160A (en) 2017-06-13

Similar Documents

Publication Publication Date Title
TWI463986B (en) New use of lactobacillus plantarum cmu995 strain
CN104758318B (en) A kind of compound formulation and preparation method thereof reducing diarrhea of weaned piglets rate
CA2689862A1 (en) Probiotic compositions comprising propionibacterium and uses thereof
CN103719623B (en) Prevention or alleviate weanling pig stress the Chinese herbal feed additive and preparation method thereof of syndrome
JPH0195726A (en) Reconstructive dryed product for direct ingestion and method for its manufacture
CN105685970A (en) Compound nutritious food capable of improving whole digestive tract
CN106860483A (en) Probiotics complex composition and application containing Bifidobacterium and lactobacillus acidophilus
CN110917174A (en) Oral probiotic capsule for regulating balance of female vaginal flora and preparation method thereof
KR101000364B1 (en) Double-coating methods for enhancing viability
EP3897678A1 (en) Probiotic combination for treatment of allergic disorders
CN106071173A (en) A kind of feed additive for milch cow postpartum recovery and preparation method thereof
CN104171743B (en) A kind of feed additive for chickens and preparation method thereof
CN115671132B (en) Composition of probiotics and prebiotics and application thereof
CN104524453B (en) Can be used for preventing fermentation of Chinese herbal medicine preparation and the application thereof of broiler coli-infection
CN113876935A (en) Composition for treating intestinal diseases of pets and application thereof
Lassen Vitamin A deficiency and resistance against a specific infection. Preliminary report
CN106889615B (en) Microelement selenium dietary supplement with function of regulating intestinal flora and application thereof
CN106820160B (en) Microelement selenium dietary supplement with function of relieving salmonella induced enteritis
CN114933984B (en) Probiotic and prebiotic composite composition for promoting in-vivo plasticizer and heavy metal discharge and preparation method thereof
CN105995981A (en) Full-nutrient formula food containing probiotic microcapsules and having special medical purpose and preparation method thereof
CN110384743A (en) A kind of complex prebiotics chewable tablets inhibiting helicobacter pylori
CN112704234A (en) Nutritional composition containing composite peptide and probiotics and preparation method thereof
WO2024055976A1 (en) Composition of sunflower seed protein and use thereof
CN111821322A (en) Poultry micro-ecological oral preparation capable of replacing antibiotics and application thereof
CN112716942B (en) Indole and application of duck feed containing indole in maintaining duck intestinal health and/or preventing necrotic enteritis of ducks

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant