CN106820160A - A kind of trace elements of selenium dietary supplements with alleviation salmonella induction enteritis function - Google Patents
A kind of trace elements of selenium dietary supplements with alleviation salmonella induction enteritis function Download PDFInfo
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- CN106820160A CN106820160A CN201710066209.1A CN201710066209A CN106820160A CN 106820160 A CN106820160 A CN 106820160A CN 201710066209 A CN201710066209 A CN 201710066209A CN 106820160 A CN106820160 A CN 106820160A
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- Prior art keywords
- dietary supplements
- salmonella
- feed
- pharmaceutical composition
- selenium
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Links
- 241000607142 Salmonella Species 0.000 title claims abstract description 40
- 239000011669 selenium Substances 0.000 title claims abstract description 38
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 32
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 32
- 235000015872 dietary supplement Nutrition 0.000 title claims abstract description 28
- 230000006698 induction Effects 0.000 title claims abstract description 10
- 239000011573 trace mineral Substances 0.000 title abstract description 18
- 235000013619 trace mineral Nutrition 0.000 title abstract description 18
- 208000004232 Enteritis Diseases 0.000 title abstract description 10
- 229940091258 selenium supplement Drugs 0.000 claims abstract description 31
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims abstract description 19
- 235000015921 sodium selenite Nutrition 0.000 claims abstract description 19
- 229960001471 sodium selenite Drugs 0.000 claims abstract description 18
- 239000011781 sodium selenite Substances 0.000 claims abstract description 18
- 239000008194 pharmaceutical composition Substances 0.000 claims abstract description 17
- 239000000047 product Substances 0.000 claims abstract description 8
- 241000209140 Triticum Species 0.000 claims abstract description 6
- 235000021307 Triticum Nutrition 0.000 claims abstract description 6
- 239000003814 drug Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 7
- 239000002775 capsule Substances 0.000 claims description 6
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- 229910021641 deionized water Inorganic materials 0.000 claims description 4
- 238000005469 granulation Methods 0.000 claims description 4
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- 238000002360 preparation method Methods 0.000 claims description 3
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- 239000004615 ingredient Substances 0.000 abstract 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 17
- 125000003748 selenium group Chemical group *[Se]* 0.000 description 9
- 230000000694 effects Effects 0.000 description 6
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- 239000008188 pellet Substances 0.000 description 3
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- 206010039447 salmonellosis Diseases 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 206010012735 Diarrhoea Diseases 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
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- 239000002158 endotoxin Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
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- 230000036541 health Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000314 lubricant Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
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- 239000003381 stabilizer Substances 0.000 description 2
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- 235000019698 starch Nutrition 0.000 description 2
- 230000035882 stress Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 239000012049 topical pharmaceutical composition Substances 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- 206010000087 Abdominal pain upper Diseases 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 241000194020 Streptococcus thermophilus Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- KIPLYOUQVMMOHB-MXWBXKMOSA-L [Ca++].CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O.CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O Chemical compound [Ca++].CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O.CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O KIPLYOUQVMMOHB-MXWBXKMOSA-L 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
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- 239000008280 blood Substances 0.000 description 1
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- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
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- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000007884 disintegrant Substances 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 208000028104 epidemic louse-borne typhus Diseases 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
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- 230000000977 initiatory effect Effects 0.000 description 1
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- 239000002054 inoculum Substances 0.000 description 1
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- 210000004279 orbit Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
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- 231100000572 poisoning Toxicity 0.000 description 1
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- 150000003254 radicals Chemical class 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
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- 239000003826 tablet Substances 0.000 description 1
- 229940063650 terramycin Drugs 0.000 description 1
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- 210000001835 viscera Anatomy 0.000 description 1
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- 239000008215 water for injection Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1322—Inorganic compounds; Minerals, including organic salts thereof, oligo-elements; Amino-acids, peptides, protein-hydrolysates or derivatives; Nucleic acids or derivatives; Yeast extract or autolysate; Vitamins; Antibiotics; Bacteriocins
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D13/00—Finished or partly finished bakery products
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
- A21D2/02—Treatment of flour or dough by adding materials thereto before or during baking by adding inorganic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/04—Sulfur, selenium or tellurium; Compounds thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a kind of with the trace elements of selenium dietary supplements for alleviating salmonella induction enteritis function, belong to food technology field.The main beneficiating ingredient of trace elements of selenium dietary supplements of the invention is sodium selenite, and it can suppress salmonella and mouse immune organ is infected, and significantly improve mouse body Antioxidant Indexes.Trace elements of selenium dietary supplements of the invention is used to prepare has acidified milk, wheat products and the pharmaceutical composition of alleviating salmonella induction enteritis function, with application prospect widely.
Description
Technical field
The present invention relates to a kind of with the trace elements of selenium dietary supplements for alleviating salmonella induction enteritis function, belong to
Food technology field.
Background technology
Salmonella typhimurium is that one kind can infect many mammiferous hosts, triggers intestines problem and diarrhoea even dead
The pathogenic bacteria for dying.It belongs to without host specificity but has the pathogen of invasive, it was reported that the food of the developed country such as Japan, U.S.
40~80% is to infect initiation by salmonella in thing poisoning thing., the salmonella that the World Health Organization announces in 1981
There are more than 2000 kinds, children's salmonella infection mouse typhus of wherein reported literature has accounted for 53-80%.Salmonella typhimurium property
Shape is：It is aerobic, without gemma, Gram-negative bacteria amphitrichous.Adapt to extraneous viability strong, be widely distributed in nature
In.The common infection sources has：Temporarily without the salmonella carrier of illness, the internal organ of poultry and other animals are contaminated strong
Health livestock and poultry cultivation and egg and egg products etc..Alimentary canal is the primary approach that salmonella is infected to body.Intake is by Salmonella
The food and water that bacterium polluted can all cause infection morbidity.After salmonella enters enteron aisle, caused a disease by endotoxin.Part is husky
Salmonella can be colonized door in enteron aisle, and most of salmonella is then excreted by excrement.Salmonella it is latent
Phase is indefinite, and most common Clinical types are generated heat for chilly, vomiting, diarrhoea, stomachache, the enteritis phenomenon such as stool water sample.Now for
The effective ways of salmonella infection are to use antibiotic, such as terramycin, chloramphenicol, and are aided with symptomatic treatment.Furans and
Sulfamido also has certain effect.
With widely using for antibiotic, salmonella particularly salmonella typhimurium is also big to the drug resistance of antibiotic
Width strengthens.Although only small part salmonella (or spontaneous mutation) can possess drug resistance under the induction of antibiotic,
After the widely using of antibiotic, this small part bacterium obtains amount reproduction, as dominant microflora and causes body enteritis.Therefore,
We need find one can more preferably prevent or control salmonella infection body and do not have the new method of side effect.
The content of the invention
In order to solve the above problems, the invention provides for alleviating salmonella infecting and salmonella to body
The trace elements of selenium dietary supplements of the illness of enteron aisle is induced, is with sodium selenite as active ingredient or principle active component.
First purpose of the invention is to provide and a kind of can alleviate salmonella infecting or salmonella to body
The dietary supplements of the illness of enteron aisle is induced, the dietary supplements is with sodium selenite as principle active component.
In one embodiment, the dietary supplements is feed, beverage, wheat flour, wheat products etc..
In one embodiment, it is additionally included in acceptable carrier in feed in the feed.
In one embodiment, the feed is granular pattern feed.
In one embodiment, the feed is mouse feed.
In one embodiment, the preparation of the feed：Will be sodium selenite dissolving in deionized water, then with feeding
Material is mixed, granulation；Wherein Se content is 0.15~2.00mg/kg feeds.
In one embodiment, Se content is 0.40~1.00mg/kg feeds in the feed.
In one embodiment, the beverage is milk-contained drink, such as acidified milk.
In one embodiment, the wheat products are including bread etc..
Second object of the present invention is to provide and a kind of can alleviate salmonella infecting or salmonella to body
The medicine or pharmaceutical composition of the illness of enteron aisle are induced, the medicine or pharmaceutical composition are with sodium selenite as main effective
Composition.
In one embodiment, described pharmaceutical composition also includes pharmaceutically acceptable carrier.
In one embodiment, the sodium selenite in described pharmaceutical composition is the sodium selenite as obtained by freeze-drying
Powder.
In one embodiment, medicine or pharmaceutical composition can be configured to several formulation, wherein containing pharmacy neck
Some excipient commonly used in domain；For example, oral formulations (such as tablet, capsule, solution or suspension)；The preparation of injectable
(dried powder of such as solution or suspension of injectable, or injectable adds water for injection to make immediately before the injection
With)；Topical formulations (such as ointment or solution).
In one embodiment, described pharmaceutical composition is capsule or tablet.
In one embodiment, the carrier for pharmaceutical composition of the present invention is available common class in pharmaceutical field
Type, including：The adhesive of oral formulations, lubricant, disintegrant, cosolvent, diluent, stabilizer, suspending agent, non-pigment,
Flavouring etc.；Preservative, solubilizer, stabilizer of injectable formulation etc.；The matrix of topical formulations, diluent, lubricant,
Preservative etc..
In one embodiment, described pharmaceutical composition is put together with label；Selenium intake is recorded on the label
In 200~400 μ g/d.
Sodium selenite of the present invention all meets edible demand, and form is in crystal type powder, water is dissolved in, insoluble in second
Alcohol.Fusing point is 350 DEG C, purity>99.99%, molecular weight is 119.9634, stable in the air, to humidity sensitive, easily crystallization.
Trace elements of selenium dietary supplements of the invention or medicine or pharmaceutical composition have following beneficial characteristics：
1st, salmonella can be suppressed to infect body tissue；
2nd, the oxidation resistance of body can be improved.
Present invention demonstrates that, as trace elements of selenium dietary supplements 200~400 μ g/d of addition, can effectively suppress husky
Door infects by Salmonella to Different Organs, improves the immunocompetence of enteron aisle, mitigates because salmonella infects the oxidation brought to body
Stress damage.
Brief description of the drawings
Fig. 1 is feed making method flow.
Specific embodiment
Embodiment 1：It is prepared by mouse feed
Feed making method of the invention is as shown in Figure 1.
Sodium selenite dissolving in deionized water, then mixed with feed, granulation.0.15,0.40,1.00 is weighed respectively
With 2.00mg sodium selenites, it is dissolved in the deionized water of 200mL, pours into the feedstuff of 1kg, mixing granulation.Form selenium
Content is the feed of 0.15,0.40,1.00 and 2.00mg/kg, is respectively designated as control group, selenium-rich group, selenium group high and high selenium
Group.They are respectively 50,200,400 and 800ug/d equivalent to the intake of people.In implementation of the invention, the composition of feed is such as
Shown in table 1 below, table 2：
The basal feed of table 1 is constituted and content
The basal feed of table 2 is constituted and nutrient composition content
Embodiment 2：Trace elements of selenium dietary supplements infects the influence of mouse tissue to salmonella
40 SPF of four week old grades of mouse are randomly divided into 4 groups, every group 10.Being grouped is respectively, control group, selenium-rich group,
Selenium group high and high selenium group.Every group of difference feeding Se content is the feed of 0.15,0.40,1.00 and 2.00ppm.Mouse is freely adopted
Food drinking-water, feeding 2 months.It is extensive after the streptomysin of bore gavage 20mg by all mouse 4h fasting for solids and liquids after two months terminate
The free diet of multiple mouse.After streptomysin treatment 20h, 4h fasting for solids and liquids, 5 diluted with PBS through bore gavage again
×107CFU salmonella typhimuriums SL1344.Whole mouse are killed after 48h to be dissected, and take spleen and lymphonodi mesenterici
(mLN) cleaned with physiological saline, and in the physiological saline containing 1% Triton X-100 be homogenized, by homogenate be applied to containing
On the Mai Kangkai plating mediums of 0.5% streptomysin, wherein salmonella quantity is counted.
The salmonella of table 3 infects degree to immune organ
Note：*:Compare P with control group<0.05.
Salmonella after body enteron aisle is intruded into, in infecting the immune organ around alimentary canal, to device is immunized
Official causes oxidativestress damage.The as shown by data of table 3, either in spleen or lymphonodi mesenterici, selenium-rich group and selenium high
The salmonella quantity of group is considerably less than control group, and this explanation present invention can effectively suppress husky in selenium-rich group and selenium group high
Door infects by Salmonella to Immune Organs of Body, so as to alleviate the damage that salmonella induction enteritis is caused to body.
Embodiment 3：Influence of the trace elements of selenium dietary supplements to anti-oxidative ability of mice
According to the mouse experiment described in embodiment 1, after experiment terminates, eye socket takes blood and puts to death mouse, and the liver for taking mouse is used
The physiological saline cleaning of precooling, homogenate.SOD (superoxide dismutase), MDA (MDA) and T-AOC are (total anti-in determining liver
Oxidability) etc. Antioxidant Indexes.Test result such as following table.
The anti-oxidant measure of the mouse liver of table 4
Note：* represent that the group has significant difference, p compared with control group<0.05；The group that * represents compares with control group to be had
Very big significant difference, p<0.01.
Salmonella can secrete endotoxin after body is invaded by own metabolism, increase the oxidative stress of body cell
Effect.The enteritis that it is induced can also increase the concentration of active oxygen in body, and bigger oxidativestress damage is caused to body.SOD
(superoxide dismutase) is a kind of active material in body, and its main function is to eliminate organism in metabolic processes
The harmful substances such as the free radical of middle generation, repair because of the cell that oxidative stress effect is damaged, and T-AOC (TAC) is to comment
One overall target of valency antioxidant ability of organism, when the oxidation resistance of body tissue is stronger, the numerical value of T-AOC is got over
Greatly.MDA (MDA) is that biological interior free yl occurs the significant product of peroxidization with lipid, is biological vivo oxidation
The index of stress damage.
Can be seen that trace elements of selenium dietary supplements of the invention by the result of table 4 can effectively improve mouse antioxygen
Change ability.SOD is apparently higher than control group wherein in selenium-rich group mouse liver, although selenium group high compares without obvious with control group
Difference, but also slightly rise.This two groups in the level of T-AOC, all apparently higher than control group, illustrate this two groups total
Oxidation resistance is higher than control group, and the oxidation resistance of body is strengthened.From MDA values as can be seen that the MDA of selenium group high
Value will be significantly lower than control group, although selenium-rich group and control group are relatively without obvious difference, but MDA concentration has also dropped
It is low, this illustrate this two groups of mouse receive salmonella oxidative stress effect the no control group of influence it is obvious.High selenium group is presented
Opposite trend.Data above absolutely proves that trace elements of selenium dietary supplements of the invention is true in selenium-rich group and selenium group high
It is real to alleviate because salmonella infects oxidative stress effect of the induction enteritis to body band.
Application Example 1：Prepare the acidified milk containing trace elements of selenium dietary supplement
After fresh milk sugaring dissolving, a certain amount of sodium selenite is added, make its concentration for 200 μ g/kg, in 60 DEG C, 20MPa conditions
Under carry out homogeneous, the then sterilization 5min under the conditions of 95 DEG C cools to 35 DEG C, adds business dry powder leaven bulgarian milk bar
Bacterium and business dry powder leaven streptococcus thermophilus, the inoculum concentration of the Mixed Microbes is the 0.03% of fresh milk weight, is mixed, at 40 DEG C
Lower heat-preservation fermentation, after curdled milk, 24h is refrigerated at 4 DEG C of temperature, obtains the Selenium-enriched fermentation breast.
Application Example 2：Prepare the bread of content trace elements of selenium dietary supplement
The sodium selenite of 10g white granulated sugars and 200 μ g is dissolved in the warm water of 300ml (41~45 DEG C), dry ferment is sprinkled upon
Waterborne, stirring stands 10-15min.Then water is poured into 1kg flour, is mixed, kneaded dough, 60min is placed at room temperature.Use fist
Head flattens dough and excludes wherein bubble, and dough is divided into etc. into size several pieces, then will small dough holding chamber temperature is lower continues fermentation extremely
The twice of original volumes is big, and fermentation time is according to depending on room temperature, being between 60min~120min.Apply a little yellow in dough surface
Oil, the 30~35min of oven for baking with 220 DEG C.Room temperature is cooled to, that is, obtains described selenium-enriched bread.
Application Example 3：Prepare the capsule product containing trace elements of selenium dietary supplements
The μ g of sodium selenite 200 are weighed, is added in 3% sodium alginate soln by weight, be sufficiently stirred for dissolving, so
Micelle will be formed in this mixed liquor by weight 2% calcium chloride solution afterwards, solidification 30min is stood, micelle is collected by filtration, will collected
Freeze-drying 48h is carried out to micelle, the pulvis containing sodium selenite is obtained, this pulvis is loaded the medicine sold in the market
With in capsule, obtaining described capsule product.
Application Example 4：Prepare the tablet containing trace elements of selenium dietary supplements
Weigh respectively using freeze-drying method prepare the weight portion of sodium selenite pulvis 25.7, the weight portion of starch 55.0,
The weight portion of cellulose derivative 4.5, the weight portion of sodium carboxymethyl starch 12.0, the weight portion of talcum powder 0.8, the weight portion of sucrose 1.0 with
The weight portion of water 1.0, is fully mixed, and wet granular is made using conventional method, is then produced using such as Zhongnan (South Contral) Pharmacentical Machinery Fectory
Tablet press machine carries out compressing tablet, then using such as Qingzhou City Yikang Chinese traditional medicine machine Co., Ltd produce small drug drier in enter
Row drying, then it is packaged to be tablet of the invention.
Application Example 5：Prepare the mouse pellet containing trace elements of selenium dietary supplements
The sodium selenite of 0.4~1mg is added into 1kg powdery premixing feedstuffs, is fully mixed, with minitype fodder-pelleting machine
Pelletized, pelleting temperature is 65 DEG C, obtained selenium-rich pellet, gained pellet Se content is 0.4~1mg/kg.
Although the present invention is disclosed as above with preferred embodiment, it is not limited to the present invention, any to be familiar with this skill
The people of art, without departing from the spirit and scope of the present invention, can do various changes with modification, therefore protection model of the invention
Enclose being defined of being defined by claims.
Claims (10)
1. it is a kind of can alleviate salmonella to body infect or salmonella induction enteron aisle illness dietary supplements,
Characterized in that, the dietary supplements is with sodium selenite as principle active component.
2. dietary supplements according to claim 1, it is characterised in that the dietary supplements is feed, beverage, wheat
Powder, wheat products etc..
3. dietary supplements according to claim 2, it is characterised in that be additionally included in the feed in feed and be subjected to
Carrier.
4. dietary supplements according to claim 2, it is characterised in that the feed is granular pattern feed.
5. dietary supplements according to claim 2, it is characterised in that the preparation of the feed：Will sodium selenite is molten
Solution in deionized water, is then mixed with feed, granulation；Wherein Se content is 0.15~2.00mg/kg feeds.
6. dietary supplements according to claim 2, it is characterised in that in the feed Se content be 0.40~
1.00mg/kg feeds.
7. it is a kind of can alleviate salmonella to body infect or salmonella induction enteron aisle illness medicine or medicine
Compositions, it is characterised in that the medicine or pharmaceutical composition are with sodium selenite as principle active component.
8. pharmaceutical composition according to claim 7, it is characterised in that described pharmaceutical composition also includes pharmaceutically connecing
The carrier received.
9. pharmaceutical composition according to claim 7, it is characterised in that described pharmaceutical composition is capsule or piece
Agent.
10. pharmaceutical composition according to claim 7, it is characterised in that described pharmaceutical composition is put together with label；
Selenium intake is recorded on the label in 200~400 μ g/d.
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Citations (2)
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|---|---|---|---|---|
| CN102573523A (en) * | 2009-08-11 | 2012-07-11 | 金娟辰 | Health supplement food, feed, and pharmaceutical composition comprising chia seed and maca, and manufacturing method thereof |
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2017
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|---|---|---|---|---|
| CN102573523A (en) * | 2009-08-11 | 2012-07-11 | 金娟辰 | Health supplement food, feed, and pharmaceutical composition comprising chia seed and maca, and manufacturing method thereof |
| CN105248932A (en) * | 2015-08-25 | 2016-01-20 | 四川农业大学 | Trace element premix capable of reducing propagation and invasion of Salmonella Enteritidis phage type 4 in laying hen and preparation method thereof |
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