CN109258961A - A kind of mouse feed and its preparation method and application for preventing and treating Salmonella Typhimurium Infection - Google Patents

A kind of mouse feed and its preparation method and application for preventing and treating Salmonella Typhimurium Infection Download PDF

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Publication number
CN109258961A
CN109258961A CN201811202353.4A CN201811202353A CN109258961A CN 109258961 A CN109258961 A CN 109258961A CN 201811202353 A CN201811202353 A CN 201811202353A CN 109258961 A CN109258961 A CN 109258961A
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feed
salmonella typhimurium
mouse
mouse feed
gelatin
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方国臻
黄晶
李萍
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Tianjin University of Science and Technology
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Tianjin University of Science and Technology
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Priority to CN201811202353.4A priority Critical patent/CN109258961A/en
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/50—Feeding-stuffs specially adapted for particular animals for rodents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/288—Taraxacum (dandelion)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/46—Eucommiaceae (Eucommia family), e.g. hardy rubber tree
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/537—Salvia (sage)
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/57—Magnoliaceae (Magnolia family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
    • A61K36/605—Morus (mulberry)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8962—Allium, e.g. garden onion, leek, garlic or chives
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/906—Zingiberaceae (Ginger family)
    • A61K36/9068—Zingiber, e.g. garden ginger
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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Abstract

The present invention relates to a kind of mouse feeds for preventing and treating Salmonella Typhimurium Infection, and it is as follows that raw material forms g/kg: cornstarch 340~390, resistant starch 55~105, fish meal 240~280, sucrose 80~100, soybean oil 40~50, wheat bran 50~60, composite mineral matter premix 20~35, complex vitamin premix 10~20, DL- METHIONINE 2~3, l-cysteine 2~3, choline chloride 1~2, lipotropin 1~2, gelatin 20~50.This mouse feed has effects that dampness-eliminating and dysentery-stopping, antibacterial anti-inflammatory, can be used for preventing and treating the appearance of experiment mice salmonellosis problem, solves the problems, such as that at present excessively abuse of antibiotics prevents and treats salmonellosis and causes the appearance of endurance strain.A kind of green safe dietetic treatment is provided, the present invention is simple to equipment requirement, significant effect, and it is safe, without side-effects, it is suitble to large-scale promotion application.

Description

A kind of mouse feed and preparation method thereof that preventing and treating Salmonella Typhimurium Infection and Using
Technical field
The invention belongs to field of feed, are related to a kind of experiment mice feed, especially a kind of prevention and treatment mouse typhus sramana The mouse feed and its preparation method and application of Salmonella infection.
Background technique
Salmonella is a kind of pathogenic entero becteria, and can invade host intestine epithelial cell leads to serious clinical disease, For example, vomiting, diarrhea, intestinal inflammatory etc..20%~30% colon cancer is as caused by intestinal inflammatory, for this according to estimates Reason-study diet intervention has great importance to the effect for alleviating enteron aisle.
The potential prebiotic effect of resistant starch and functional characteristic make it obtain more and more concerns, it is largely present in It is a kind of important dietary fiber in the dietary materials of nondigestible carbohydrate, big gut health, prevention colitis can be promoted And colon cancer.A large amount of research has shown that resistant starch has many physiological properties beneficial to human body, while resistant starch is got over Come more for producing the food of high microsteping content low in calories.The diet that resistant starch also be used to improve food simultaneously contains Amount, while there are the potentiality for reducing blood glucose.The way that resistant starch passes through and increases the content of total dietary fiber in food, reduces energy Diameter improves intestinal health.Most importantly resistant starch can significantly improve the content of enteron aisle Short-Chain Fatty Acids, short-chain fat Acid can build the low bad border pH for enteron aisle not only to the growth and breeding for inhibiting most of pathogenic bacteria, can also promote on intestines Epithelial cell proliferation repairs gut barrier and is enriched with beneficial bacteria of intestinal tract.
At present China prevention and treatment experimental mouse intestinal inflammatory as caused by pathogenic bacteria main means be using antibiotic medicine, this Kind of drug can upset the balance of mouse itself intestinal flora and reduce mouse immunity, diet intervention as a kind of safety, green, Novel therapeutic means without side-effects but rarely have research.The present invention, which has developed a kind of feed and can be used as diet intervention, alleviates sense Contaminate intestinal inflammatory caused by salmonella typhimurium.
By retrieval, following two patent publication us related with the present patent application are found:
1, a kind of chicken feed and preparation method thereof for preventing and treating newcastle disease, the present invention provide a kind of new for preventing and treating chicken The chicken feed and preparation method thereof of city epidemic disease, by the raw material of following parts by weight form corn is a, pine needle meal is a, wheat is a, Soya bean is a, rape cake is a, wheat drum is a, soya-bean cake is a, a, the first bud grass meal of fine rice bran is a, conch meal is a, phosphorus Sour hydrogen calcium is a, salt is a, composite trace element is a, compound multivitamin is a, Chinese herb feed additive is a.It is a kind of For prevent and treat newcastle disease chicken feed have it is clearing heat and detoxicating, replenish qi to invigorate the spleen, strengthening vital QI to eliminate pathogenic factors, antibacterial anti-inflammatory, antiviral effect. It not only can be that the nutrition such as sufficient protein, vitamin, microelement are provided in chicken growth course by feeding this kind of feed Ingredient, promotes the growth and development of body, and can also be used in the effect for preventing and treating the appearance of newcastle disease problem.
2, a kind of Chinese herbal medicine mixed feed and preparation method thereof for improving beef cattle immunity, the present invention relates to a kind of raising meat Chinese herbal medicine mixed feed of ox immunity and preparation method thereof is made of the raw material of following parts by weight: 30~60 parts of corn, dregs of beans 5~15 parts, 10~20 parts of wheat bran, 5~15 parts of cottonseed cake, 1~5 part of bone meal, 15~45 parts of Chinese herb feed additive, salt 0.5~ 3.5 parts, 0.1~1 part of premix material additive, wherein Chinese herb feed additive is made of the raw material of following weight parts: feed mulberry 30~ 60 parts, octagonal 0.5~3.5 part, 1~6 part of Radix Salviae Miltiorrhizae, 1~6 part of ginger, 1~6 part of garlic, 1~6 part of Cortex Eucommiae, 1~6 part of dandelion. Rationally, lower production costs are used for beef cattle feeding, can effectively mention the various nutritional ingredient proportions of Chinese herbal medicine mixed feed of the invention High feed intake, disease-resistant growth-promoting, enhancing immunity of organisms and the effect for improving oxidation resistance, to overcome nutrition in the prior art The problems such as ingredient shortage, nutrient content deficiency, drug resistance and environmental pollution, be a kind of mixed feed of green natural.
By comparing both the above feed formula, it can be seen that mouse feed of the invention is different for animal species, matches Side is at low cost, and nutrition is more comprehensive, and operability is stronger, is conducive to large-scale promotion, and the present invention have developed one kind for the first time can To prevent and treat the feed of the mouse intestinal inflammation as caused by salmonella typhimurium.
Summary of the invention
Place that the purpose of the present invention is to overcome the deficiency in the prior art provides a kind of prevention and treatment Salmonella Typhimurium Infection Mouse feed and its preparation method and application, the mouse feed have effects that dampness-eliminating and dysentery-stopping, antibacterial anti-inflammatory, can be used for preventing With the appearance of Experiment on therapy mouse salmonellosis problem, solves at present excessively abuse of antibiotics prevention and treatment salmonella Appearance that is sick and causing endurance strain problem.A kind of green safe dietetic treatment is provided, the present invention is simple to equipment requirement, Significant effect, it is safe, without side-effects, it is suitble to large-scale promotion application.
The technical solution adopted by the present invention to solve the technical problems is:
A kind of mouse feed for preventing and treating Salmonella Typhimurium Infection, it is as follows that raw material forms g/kg:
Moreover, the resistant starch is RS3 type resistant starch.
Moreover, the gelatin is food-grade gelatin.
Moreover, the composite mineral matter premix combined by milk powder and animal's liver powder according to the ratio of mass ratio 3:4 and At.
Moreover, the complex vitamin premix is composed of sorghum flour and soy meal according to the ratio of mass ratio 1:1.
A kind of preparation method of the mouse feed of prevention and treatment Salmonella Typhimurium Infection as described above, steps are as follows:
(1) required various materials are weighed according to formula；
(2) mixing weighs the good all materials in addition to gelatin；
(3) the material mixed is crossed into 60-80 mesh；
(4) aqueous gelatin solution is made into water by load weighted gelatin is molten, the gelatin: the mass ratio of water is 2~5:50, The water-bath 30 minutes under conditions of 70 DEG C；
(5) the material after being sieved pours into blender, the aqueous gelatin solution after pouring into water-bath while stirring；
It (6) is 3-5 centimetres by length is twisted into good feed dough, diameter is 1 centimetre cylindric；
(7) the feed (6) step prepared is put into baking oven, 65 DEG C, until mouse feed is all dried from inside to outside, takes out Up to the mouse feed of prevention and treatment Salmonella Typhimurium Infection.The mouse of prevention and treatment Salmonella Typhimurium Infection as described above is raised Expect alleviating caused by infection salmonella typhimurium the application in terms of intestinal inflammatory as diet intervention.
The advantages of present invention obtains and good effect are as follows:
1, mouse feed of the present invention has effects that dampness-eliminating and dysentery-stopping, antibacterial anti-inflammatory, and it is small to can be used for preventing and treating experiment The appearance of mouse salmonellosis problem solves at present excessively abuse of antibiotics prevention and treatment salmonellosis and causes drug resistance The appearance of property bacterial strain problem.There is provided a kind of green safe dietetic treatment, the present invention is simple to equipment requirement, significant effect, peace Entirely, without side-effects, it is suitble to large-scale promotion application；Feed of the present invention can be used as diet intervention and alleviate infection Salmonella typhimurium Microbial intestinal inflammatory.
2, mouse feed of the present invention is added to resistant starch compared with common mouse feed formula, can be effectively relieved by mouse Intestinal inflammatory caused by salmonella typhi infects increases the content of enteron aisle Short-Chain Fatty Acids, caused by reducing infection sramana The death rate.
3, the composite mineral matter premix that feed of the present invention uses is by milk powder and animal's liver powder according to the ratio of mass ratio 3:4 Example is composed, it ensure that the full nutrition of final finished and good mouthfeel.
4, the complex vitamin premix that feed of the present invention uses is by sorghum flour and soy meal according to the ratio of mass ratio 1:1 It is composed, it ensure that the full nutrition of final finished and good physiological function.
5, the present invention uses aqueous gelatin solution when mixing all ingredients in the preparation, it is ensured that can reach small Flintiness needed for mouse feed is conducive to mouse molar teeth and palatability.
6, zoopery discovery is done with feed of the present invention, feed of the present invention is really to caused by infection salmonella typhimurium Mouse intestinal inflammation has prevention and relaxation effect.Experimental data shows that resistant starch feed can significantly improve pathogenic bacteria and cause Mouse intestinal tissue morphology damage, increase enteron aisle Short-Chain Fatty Acids content, mitigate inflammation, adjust intestinal flora, effectively Prevent intestinal inflammatory caused by salmonella.
Detailed description of the invention
Fig. 1 is each group experiment mice small intestine morphological contrast map in the present invention；
Fig. 2 is the concentration map of inflammatory factor in each group experiment mice serum in the present invention；Wherein, Fig. 2 .1 is inflammatory factor TNF-α contains spirogram, and Fig. 2 .2 is that inflammatory factor IL-1 β contains spirogram, and Fig. 2 .3 is that inflammatory factor IL-6 contains spirogram, and Fig. 2 .4 contains for NO Spirogram；
Fig. 3 is that each group experiment mice excrement Short-Chain Fatty Acids contain spirogram in the present invention；
Fig. 4 is the measurement chart of mouse death rate in the present invention.
Specific embodiment
The embodiment of the present invention is described in detail below, it should be noted that the present embodiment is narrative, is not limited , this does not limit the scope of protection of the present invention.
Raw material used in the present invention is unless otherwise specified conventional commercial product；Used in the present invention Method is unless otherwise specified the conventional method of this field.
Embodiment 1
A kind of mouse feed for preventing and treating Salmonella Typhimurium Infection, it is as follows that raw material forms g/kg:
Cornstarch 340g, RS3 type resistant starch 105g, it fish meal 280g, sucrose 100g, soybean oil 50g, wheat bran 50g, answers Close mineral substance premix 35g, complex vitamin premix 10g, DL- METHIONINE 3g, l-cysteine 3g, choline chloride 2g, weight wine Stone acid choline 2g, gelatin 20g.
The preparation method of the mouse feed of above-mentioned prevention and treatment Salmonella Typhimurium Infection carries out in accordance with the following steps:
(1) required various materials are weighed according to formula；
(2) mixing weighs the good all materials in addition to gelatin；
(3) the material mixed is crossed into 60-80 mesh；
(4) aqueous gelatin solution is made into 500ml water by load weighted food-grade gelatin is molten, water-bath under conditions of 70 DEG C 30 minutes；
(5) the material after being sieved pours into blender, the aqueous gelatin solution after pouring into water-bath while stirring；
It (6) is 3-5 centimetres by length is twisted into good feed dough, diameter is 1 centimetre cylindric；
(7) the feed (6) step prepared is put into baking oven, 65 DEG C, until mouse feed is all dried from inside to outside, takes out Up to the mouse feed of prevention and treatment Salmonella Typhimurium Infection.
Embodiment 2
A kind of mouse feed for preventing and treating Salmonella Typhimurium Infection, it is as follows that raw material forms g/kg:
Cornstarch 390g, RS3 type resistant starch 55g, it fish meal 280g, sucrose 100g, soybean oil 50g, wheat bran 50g, answers Close mineral substance premix 35g, complex vitamin premix 10g, DL- METHIONINE 3g, l-cysteine 3g, choline chloride 2g, weight wine Stone acid choline 2g, gelatin 20g.
The preparation method of the mouse feed of above-mentioned prevention and treatment Salmonella Typhimurium Infection carries out in accordance with the following steps:
(1) required various materials are weighed according to formula；
(2) mixing weighs the good all materials in addition to gelatin；
(3) the material mixed is crossed into 60-80 mesh；
(4) aqueous gelatin solution is made into 500ml water by load weighted food-grade gelatin is molten, water-bath under conditions of 70 DEG C 30 minutes；
(5) the material after being sieved pours into blender, the aqueous gelatin solution after pouring into water-bath while stirring；
It (6) is 3-5 centimetres by length is twisted into good feed dough, diameter is 1 centimetre cylindric；
(7) the feed (6) step prepared is put into baking oven, 65 DEG C, until mouse feed is all dried from inside to outside, takes out Up to the mouse feed of prevention and treatment Salmonella Typhimurium Infection.
Embodiment 3
A kind of mouse feed for preventing and treating Salmonella Typhimurium Infection, it is as follows that raw material forms g/kg:
More preferably, the resistant starch is RS3 type resistant starch.
More preferably, the gelatin is food-grade gelatin.
More preferably, the composite mineral matter premix is combined by milk powder and animal's liver powder according to the ratio of mass ratio 3:4 It forms.
More preferably, the complex vitamin premix combined by sorghum flour and soy meal according to the ratio of mass ratio 1:1 and At.
A kind of preparation method of the mouse feed of prevention and treatment Salmonella Typhimurium Infection as described above, steps are as follows:
(1) required various materials are weighed according to formula；
(2) mixing weighs the good all materials in addition to gelatin；
(3) the material mixed is crossed into 60-80 mesh；
(4) aqueous gelatin solution is made into water by load weighted gelatin is molten, the gelatin: the mass ratio of water is 2~5:50, The water-bath 30 minutes under conditions of 70 DEG C；
(5) the material after being sieved pours into blender, the aqueous gelatin solution after pouring into water-bath while stirring；
It (6) is 3-5 centimetres by length is twisted into good feed dough, diameter is 1 centimetre cylindric；
(7) the feed (6) step prepared is put into baking oven, 65 DEG C, until mouse feed is all dried from inside to outside, takes out Up to the mouse feed of prevention and treatment Salmonella Typhimurium Infection.
The mouse feed that the present invention prevents and treats Salmonella Typhimurium Infection, which can be applied, is alleviating infection as diet intervention In in terms of intestinal inflammatory caused by salmonella typhimurium.
Coherent detection of the invention:
Zoopery: feed of the present invention is taken to do following experiment:
The BALB/c of 6 week old of no-special pathogen (Specific pathogens Free, SPF) i.e. SPF grade is selected in test Female mice 80, experiment mice is divided into four groups, respectively chow diet group, resistant starch group, chow diet infected group, resistance Starch infected group group, every group 20,4 one cages of mouse.Rearing conditions are: humidity 60%-80%, and temperature maintains 22 ± 2 DEG C, 12h/12h night, the free feeding of mouse and drinking-water with primary, set 4 DEG C of refrigerators and are saved for resistant starch feed every 3 days, with with It takes.After all mouse basal feeds are fed 1 week, 4 groups are randomly divided into according to weight, chow diet group and chow diet infected group are given Chow diet is given, resistant starch feed group and resistant starch feed infected group give feed of the present invention.According to this feed mode After feeding 3 weeks, i.e., (there are also 1 week laundering period before) infected group (chow diet infected group and resistant starch after experiment beginning 4 weeks Feed infected group) start stomach-filling salmonella typhimurium, the single oral gavage amount of every mouse is 2.0 × 104CFU, bacteria suspension body Product is 0.1mL/；And what non-infected group mouse (chow diet group and resistant starch feed group) filled is for making bacteria suspension Physiological saline, volume are similarly 0.1mL/ only.Continuous gavage 6 days, in the 0th day, the 3rd day and the 6th day weighing Mice Body of stomach-filling Weight simultaneously collects stool in mice and is stored in -80 DEG C of refrigerators, saves to be measured.By stomach-filling the 7th day, eyeball took blood and dissects mouse and collects back Intestines, caecum, colon tissue samples.
The variation of mouse intestinal tissue morphology is observed, as a result as shown in Figure 1.Salmonella typhimurium causes small intestines structure broken Bad, be in particular in: intestinal villi rupture even falls off, intestinal wall fracture.Feed of the present invention can significantly protect infection Salmonella The integrality of small intestines structure after bacterium.
The inflammatory factor (TNF-α, IL-1 β, IL-6, NO) in each experimental mice serum is measured, as a result as shown in Figure 2. Salmonella Typhimurium Infection causes inflammatory factor level to increase, and after feeding feed of the invention, inflammatory factor is horizontal significant Decline.
The content of each experimental mice faecal short chain fatty acid is measured, as a result as shown in figure 3, feed of the present invention can be significant Increase the content of short chain fatty acids, wherein acetic acid, butyric acid are especially pronounced.Meanwhile being found through detection, short chain fatty acids producing strains Abundance also obtains a degree of increase.Further illustrate: mouse feed of the present invention can be generated by increasing short chain fatty acids The abundance of bacterium improves short-chain fat acid content.Short chain fatty acids are the products of bacterial metabolism polysaccharide, and it is thin to cross promotion enteric epithelium The generation of the increment of born of the same parents, especially butyrate is enterocyte function, repairs Gut barrie r, alleviate salmonellal intestines Road inflammation.
The final death rate of infected group mouse is measured, as a result as shown in Figure 4, it can be seen that feed of the present invention significantly reduces The death rate of mouse after infection salmonella.
Conclude that resistant starch feed of the present invention significantly improves stool in mice Short-Chain Fatty Acids by zoopery Content, wherein acetic acid, butyric acid are especially pronounced.Short chain fatty acids are the products of bacterial metabolism polysaccharide, and enteric epithelium can be promoted thin The generation of the increment of born of the same parents, especially butyrate strengthens enterocyte function, repairs Gut barrie r, alleviates salmonella and causes Intestinal inflammatory, reduce the death rate caused by mouse infection salmonella.
Although disclosing the embodiment of the present invention for the purpose of illustration, it will be appreciated by those skilled in the art that: not Be detached from the present invention and spirit and scope of the appended claims in, various substitutions, changes and modifications be all it is possible, therefore, this The range of invention is not limited to the embodiment and attached drawing disclosure of that.

Claims (7)

1. a kind of mouse feed for preventing and treating Salmonella Typhimurium Infection, it is characterised in that: it is as follows that its raw material forms g/kg:
2. the mouse feed of prevention and treatment Salmonella Typhimurium Infection according to claim 1, it is characterised in that: the resistance Starch is RS3 type resistant starch.
3. the mouse feed of prevention and treatment Salmonella Typhimurium Infection according to claim 1, it is characterised in that: the gelatin For food-grade gelatin.
4. the mouse feed of prevention and treatment Salmonella Typhimurium Infection according to claim 1, it is characterised in that: described compound Mineral substance premix is composed of milk powder and animal's liver powder according to the ratio of mass ratio 3:4.
5. the mouse feed of prevention and treatment Salmonella Typhimurium Infection according to any one of claims 1 to 3, feature exist In: the complex vitamin premix is composed of sorghum flour and soy meal according to the ratio of mass ratio 1:1.
6. a kind of preparation side of the mouse feed of prevention and treatment Salmonella Typhimurium Infection such as described in any one of claim 1 to 5 Method, it is characterised in that: steps are as follows:
(1) required various materials are weighed according to formula；
(2) mixing weighs the good all materials in addition to gelatin；
(3) the material mixed is crossed into 60-80 mesh；
(4) aqueous gelatin solution is made into water by load weighted gelatin is molten, the gelatin: the mass ratio of water is 2~5:50,70 Water-bath 30 minutes under conditions of DEG C；
(5) the material after being sieved pours into blender, the aqueous gelatin solution after pouring into water-bath while stirring；
It (6) is 3-5 centimetres by length is twisted into good feed dough, diameter is 1 centimetre cylindric；
(7) the feed (6) step prepared is put into baking oven, 65 DEG C, until mouse feed is all dried from inside to outside, takes out to obtain the final product The mouse feed for preventing and treating Salmonella Typhimurium Infection.
7. if the mouse feed of prevention and treatment Salmonella Typhimurium Infection described in any one of claim 1 to 5 is dry as diet The pre- application alleviated in intestinal inflammatory aspect caused by infection salmonella typhimurium.
CN201811202353.4A 2018-10-16 2018-10-16 A kind of mouse feed and its preparation method and application for preventing and treating Salmonella Typhimurium Infection Pending CN109258961A (en)

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CN112219789A (en) * 2020-09-30 2021-01-15 天津科技大学 Mouse feeding method for preventing and treating salmonella typhimurium infection
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