CN106818230A - An engrafting method is changed in Magnolia wufengensis nursery stock early spring cleft grafting - Google Patents
An engrafting method is changed in Magnolia wufengensis nursery stock early spring cleft grafting Download PDFInfo
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- CN106818230A CN106818230A CN201710057062.XA CN201710057062A CN106818230A CN 106818230 A CN106818230 A CN 106818230A CN 201710057062 A CN201710057062 A CN 201710057062A CN 106818230 A CN106818230 A CN 106818230A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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- Developmental Biology & Embryology (AREA)
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Abstract
A kind of Magnolia wufengensis nursery stock early spring cleft grafting swap method, is included under 28 DEG C of temperature conditions of temperature and follows the steps below：Stock：The Flos micheliae Albae nursery stock that selection does not start vegetative activity is stock, is cut or sawed-off in grafting part, and a knife or the knife of right-angled intersection two are split from grafting part center is vertical；Scion：1 year full Magnolia wufengensis branch do not sprouted of budlet of selection does scion, and scion bottom is whittled into two bevels of equal length, it is desirable to its as deep as xylem, by knife under scion both sides cut smooth and band point, itself while thick compared with another side, 14 buds are retained in scion；Grafting：Pried open with the skin mouthful of hilt stock, stock is inserted into scion；Twine film：Scion is bundled with the joint of stock from the bottom to top with grafting membrane, bud-leaf exposes.It is an advantage of the invention that survival rate is more than 60%, for the amount reproduction of Magnolia wufengensis provides condition.
Description
Technical field
The invention belongs to seedling propagation field, it is related to the engrafting method of plant, and in particular to the cleft grafting of Magnolia wufengensis nursery stock
Change an engrafting method.
Background technology
Magnolia wufengensis (Magnolia wufengensis), also known as five peak yulans, are Beijing Forestry University Ma Lvyi professors
Et al. carry out the Magnoliacea plant novel species that finds when Plant Resource of Magnoliaceae is investigated at five peaks within 2004, only Chinese five peak is exclusive.
Magnolia wufengensis belong to megaphanerophyte high, and tree-like grace, and with extremely abundant flower morphology variation property, tapel number is from 9 valves
To 46 valves, floral color type has dark red, red with pale red different manifestations, and ornamental value is high, is splendid Evergreen garden plant
Kind.
Current Magnolia wufengensis also are not fairly well-developed out in afforestation market, and mainly reproductive survival rate is too low, because
This inventor changes a cut-grafting method using cleft grafting, increases substantially the survival rate of grafting nursery stock.
The content of the invention
An engrafting method is changed present invention aim at a kind of Magnolia wufengensis early spring cleft grafting of the survival rate more than 60% is provided, should
Method operation is relative complex, but high graft survival rate, is advantageously implemented the Magnolia wufengensis marketization.
It is to solve above technical problem, the technical scheme that the present invention is provided is to provide a kind of Magnolia wufengensis nursery stock early spring and splits
Connect under swap method is included in 2-8 DEG C of temperature conditions of temperature and follow the steps below：
Stock：The Flos micheliae Albae nursery stock that selection does not start vegetative activity is stock, is cut or sawed-off in grafting part, from grafting
Position center is vertical to split a knife or the knife of right-angled intersection two；
Scion：Selection 1 year full Magnolia wufengensis branch do not sprouted of budlet do scion, by scion bottom be whittled into two it is long
The equal bevel of degree, it is desirable to its as deep as xylem, by knife under scion both sides cut smooth and band point, itself while thick compared with another side, connect
Retain 1-4 bud on fringe；
Grafting：Pried open with the skin mouthful of hilt stock, stock is inserted into scion；
Twine film：Scion is bundled with the joint of stock from the bottom to top with grafting membrane, bud-leaf exposes.
Further, the stock also requires that interior smooth without knot, surface from top to bottom and texture is straight-through.
Further, temperature is existing to relatively low during early spring grafting, as far as possible by scion grafting side from stock to sunlight.
Further, during grafting, after scion insertion stock, the table of scion and stock joint is sprayed on exocellular polysaccharide
Face.
Compared with prior art, this graft technology has advantages below：
1st, graft survival rate, relative to the survival rate of existing 5-10%, has been greatly improved safflower beautiful more than 60%
Blue reproductive survival rate；
2nd, plant low temperature tolerance ability can be strengthened, grafting provides reference during to Magnolia wufengensis nursery stock winter or cold, right
Amount reproduction Magnolia wufengensis this rare varieties plays an important roll.
Specific embodiment
Respective description is carried out below in conjunction with instantiation.This example is that principle of the invention and feature are described, only
For explaining that the technology of the present invention is not intended to limit the scope of the technology of the present invention.
An engrafting method is changed in a kind of early spring cleft grafting of Magnolia wufengensis nursery stock, selects not yet to start the Flos micheliae Albae seedling of vegetative activity
Wood is stock, with Magnolia wufengensis current-year branch as scion, grafting work is carried out in the early spring, 2~8 DEG C of optimum temperature, but to red
Spending the further investigation of yulan nursery stock early spring graft technology and improving engrafting method has important milestone significance.
Method one
A kind of Magnolia wufengensis nursery stock early spring cleft grafting swap method, comprises the following steps that：
1) stock：The Flos micheliae Albae nursery stock that selection stock bud not yet starts vegetative activity first is stock, by stock in grafting
Cut or sawed-off at position, it is desirable to which stock is smooth without knot, surface in certain length from top to bottom and texture is straight-through, then with splitting
Knife splits a knife or the knife of right-angled intersection two from grafting part center is vertical, and deep 3 to 5 cms, specific depth is determined according to stock size
It is fixed, it is desirable to edge of a knife alignment stock middle, can not overexert, expose interpolation slit.
2) scion：1 year full branch do not sprouted of budlet of selection does scion, and scion bottom is whittled into two equal lengths
Bevel, it is desirable to its as deep as xylem, under scion bud both sides knife cut smooth and band point, itself while thick compared with another side, cut scion
Length is consistent with the depth of stock of riving, and stays 1 to 2 buds to cut short as the case may be.
3) grafting：Mouthful pried open with the skin of hilt stock, stock lightly inserted into scion, the slightly thick side of scion outwards,
Slightly thin side is inwards, it is desirable to which stock is directed at laminating or the xylem surface in alignment of the two with the forming layer of scion, and center is rived
It is most connect 2 scion buds, what cross was rived most connects 4 scion buds, it is preferred that during early spring grafting temperature it is existing to compared with
It is low, as far as possible by scion grafting side from stock to sunlight.
4) film is twined：Scion is bundled with the joint of stock from the bottom to top with special grafting membrane, it is desirable to which attention is swathed
It is tight to intake and air, scion can not be encountered in order to avoid scion is shifted, bud-leaf exposes.
Head, empirical tests are changed by above method cut-grafting Magnolia wufengensis nursery stock cleft grafting：Graft survival rate is up to more than 60%, has
Beneficial to the further raising development of Magnolia wufengensis seedling grafting technology, grafting provides ginseng when Magnolia wufengensis nursery stock winter or cold
Examine, play an important roll to amount reproduction Magnolia wufengensis nursery stock.
Preferably, method two
Also include in addition to the above methods:During grafting, after scion insertion stock, scion and stock pair are sprayed on exocellular polysaccharide
The surface at place is connect, scion is bundled with the joint of stock from the bottom to top with special grafting membrane again then, it is desirable to which attention is twined
Wrap up in it is tight can not intake and air, scion can not be encountered in order to avoid scion is shifted, bud-leaf exposes.
Exocellular polysaccharide, be some peculiar microorganisms be secreted into that cell membrane is outer during growth metabolism, easily separated with thalline,
The water-soluble polysaccharide in environment is secreted into, belongs to the secondary metabolite of microorganism.
Exocellular polysaccharide (exopolysaccharides, EPS)：It is that some peculiar microorganisms are secreted during growth metabolism
Water-soluble polysaccharide that is outer to cell membrane, easily being separated with thalline, be secreted into environment, belongs to the secondary metabolite of microorganism.
Exocellular polysaccharide microbial polysaccharide compared with plant polyose has unique and excellent physical characteristic, such as in low concentration
Under, viscosity can be increased, without forming gel；Small shearforce, can quickly increase mobility, and reduce viscosity, as long as
Shearforce is eliminated, can extensive revival rapidly.The glucan produced by leuconostoc dextranicum bacteria can as column material, big point
Sub- modifying agent etc..
Experiment：
By above engrafting method one (experimental group 1), engrafting method two (experimental group 2) and traditional engrafting method simple bud cutting method
(control group 1) and carry out Experimental comparison with wooden cortex grafting (control group 2).Each experimental group randomly chooses four skills involved in the labour
Consistent grafting workman carries out grafting to 100 plants of Magnolia wufengensis, so as to be survived to three kinds of methods by respective grafting situation
Rate and labor intensive time are compared.It is as shown in the table：
| Experimental group 1 | Experimental group 2 | Control group 1 | Control group 2 | |
| Grafting quantity | 100 | 100 | 100 | 100 |
| Survive quantity | 60 | 72 | 5 | 3 |
| Survival rate | 60% | 72% | 5% | 3% |
| The consuming time/h | 2.5 | 2.6 | 2 | 2 |
Test result indicate that：By this graft technology inventive method, one is due to eugonic Flos micheliae Albae nursery stock, life
Power is strong, easily forms callus；Two is that cleft grafting is fitted closely with the forming layer of stock, thin-walled between scion is in hibernation
Cell is adjacent to, and joint is closely sealed, produces the ability of callus strong between stock and scion, easily forms new conducting tissue, water
Point and the material such as nutrition can be transported in place；Three is that Magnolia wufengensis nursery stock spring grafting mouthful faces south, and is easy to the position daylighting of grafting bud,
Head and stock healing are changed in favourable grafting cleft grafting.60% survival rate is can reach using this kind of engrafting method, far above two other
The survival rate of control group 5% and 3%.Therefore this grafting invention solves surviving for Magnolia wufengensis nursery stock early spring grafting well
Rate, grafting and breeding when this is to Magnolia wufengensis nursery stock early spring or cold has material impact.
Experimental group 2, using exocellular polysaccharide plant surface formed viscosity, without formed gel the characteristics of, can improve
Scion and stock connective stability, can also fill the space of special grafting membrane and plant surface, again will not be to plant growth shape
Into obstruction, so as to further increase plant survival rate.
The above is only the preferred embodiment of this graft technology invention, protection scope of the present invention should be with claim institute
The scope of restriction is defined.For those skilled in the art, without departing from the spirit and scope of the present invention
Can also make it is some improvement and it is perfect, these improve and improve should also be considered as protection scope of the present invention.
Claims (4)
1. a kind of Magnolia wufengensis nursery stock early spring cleft grafting swap method, it is characterised in that：Methods described is included in 2-8 DEG C of temperature of temperature
In the case of follow the steps below：
Stock：The Flos micheliae Albae nursery stock that selection does not start vegetative activity is stock, is cut or sawed-off in grafting part, from grafting part
Center is vertical to split a knife or the knife of right-angled intersection two；
Scion：1 year full Magnolia wufengensis branch do not sprouted of budlet of selection does scion, and scion bottom is whittled into two length phases
Deng bevel, it is desirable to its as deep as xylem, by knife under scion both sides cut smooth and band point, itself while thick compared with another side, in scion
Retain 1-4 bud；
Grafting：Pried open with the skin mouthful of hilt stock, stock is inserted into scion；
Twine film：Scion is bundled with the joint of stock from the bottom to top with grafting membrane, bud-leaf exposes.
2. an engrafting method is changed in Magnolia wufengensis nursery stock according to claim 1 early spring cleft grafting, it is characterised in that:The stock
Also require that interior smooth without knot, surface from top to bottom and texture is straight-through.
3. an engrafting method is changed in the cleft grafting of Magnolia wufengensis nursery stock according to claim 1, it is characterised in that:During early spring grafting
Temperature is existing to relatively low, as far as possible by scion grafting side from stock to sunlight.
4. an engrafting method is changed in Magnolia wufengensis nursery stock according to claim 1 early spring cleft grafting, it is characterised in that:During grafting,
After scion insertion stock, the surface of scion and stock joint is sprayed on exocellular polysaccharide.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710057062.XA CN106818230A (en) | 2017-01-22 | 2017-01-22 | An engrafting method is changed in Magnolia wufengensis nursery stock early spring cleft grafting |
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| CN201710057062.XA CN106818230A (en) | 2017-01-22 | 2017-01-22 | An engrafting method is changed in Magnolia wufengensis nursery stock early spring cleft grafting |
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| CN106818230A true CN106818230A (en) | 2017-06-13 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109548505A (en) * | 2019-01-17 | 2019-04-02 | 曹春花 | The grafting and breeding technique of Magnolia wufengensis |
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| CN102318533A (en) * | 2011-08-24 | 2012-01-18 | 句容市颐和花木草专业合作社 | A kind of quick-growing cultivation method of southern magnolia |
| CN102356730A (en) * | 2011-09-15 | 2012-02-22 | 张冠一 | Rapid cultivation method for colorful Magnolia denudata |
| CN104996171A (en) * | 2015-07-08 | 2015-10-28 | 北京林业大学 | High position grafting method for magnolia wufengensis |
| CN105009946A (en) * | 2015-07-08 | 2015-11-04 | 北京林业大学 | Grafting method of Magnolia wufengensis |
| CN105940965A (en) * | 2016-05-17 | 2016-09-21 | 南京红森苗木有限公司 | Magnolia Grandiflora Linn gardening form directive culturing method |
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2017
- 2017-01-22 CN CN201710057062.XA patent/CN106818230A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102318533A (en) * | 2011-08-24 | 2012-01-18 | 句容市颐和花木草专业合作社 | A kind of quick-growing cultivation method of southern magnolia |
| CN102356730A (en) * | 2011-09-15 | 2012-02-22 | 张冠一 | Rapid cultivation method for colorful Magnolia denudata |
| CN104996171A (en) * | 2015-07-08 | 2015-10-28 | 北京林业大学 | High position grafting method for magnolia wufengensis |
| CN105009946A (en) * | 2015-07-08 | 2015-11-04 | 北京林业大学 | Grafting method of Magnolia wufengensis |
| CN105940965A (en) * | 2016-05-17 | 2016-09-21 | 南京红森苗木有限公司 | Magnolia Grandiflora Linn gardening form directive culturing method |
Non-Patent Citations (1)
| Title |
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| 蔡东元: "《苗木生产技术》", 31 August 2012, 机械工业出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109548505A (en) * | 2019-01-17 | 2019-04-02 | 曹春花 | The grafting and breeding technique of Magnolia wufengensis |
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Application publication date: 20170613 |