CN106818204A - The sub- cultigen preparation method of straw rotting fungus quarrel - Google Patents
The sub- cultigen preparation method of straw rotting fungus quarrel Download PDFInfo
- Publication number
- CN106818204A CN106818204A CN201611120011.9A CN201611120011A CN106818204A CN 106818204 A CN106818204 A CN 106818204A CN 201611120011 A CN201611120011 A CN 201611120011A CN 106818204 A CN106818204 A CN 106818204A
- Authority
- CN
- China
- Prior art keywords
- bridge
- quarrel
- rotting fungus
- caching
- straw rotting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The sub- cultigen preparation method of straw rotting fungus quarrel is related to agricultural technology field, comprises the following steps：1）, prepare pass a bridge caching material；2）, from granularity quarrel son, by quarrel son and pass a bridge caching material dry weight compare 70：30 ratio is got the raw materials ready；3）, strain make.Caching of passing a bridge expects the nutrient needed for can fully supplementing straw rotting fungus mycelial growth, amino acid, reduced sugar and the various active factor necessary to mycelial growth can be passed through again, 5mm gap bridge caching material in charge level covering promote mycelium rapid field planting, are colonized than original early 3~4d of formula.The gap bridge caching material that this method is used can allow mycelium normally to preserve wherein, be not in move back bacterium phenomenon, meet because the production schedule is changed, the inconvenience that strain is unable to long term storage, degeneration is caused.
Description
Technical field
The present invention relates to agricultural technology field, and in particular to make the formula and method of straw rotting fungus cultigen using quarrel.
Background technology
Current straw rotting fungus cultigen is mainly with wheat making, but some areas are not wheat belts, and raw material sources are difficult.
The north is the main producing region of corn, but makes strain with iblet, and one is big iblet particle, is difficult to have thorough grasp moisture, easily pollution；
Two when being that bulky grain strain is used, and in order to ensure enough germination points, certainly will increase bacterial classification usage amount；Three is iblet cultigen
After planting, maize easily triggers green mold to pollute.The maximum problem of grain strain be intolerant to storage, cover with bottle it is latter as 10 days or so
Red water occurs, mycelium moves back bacterium.No matter wheat or iblet cultigen, are all difficult preservation and transport.
The content of the invention
The technical problems to be solved by the invention are：A kind of formula and side that straw rotting fungus cultigen is made using quarrel is provided
Method.
Technical solution of the invention is：It is comprised the following steps：
1）, prepare pass a bridge caching material：
1. straw rotting fungus waste mushroom leftover is dried, powder is standby into 5~10mm；
2. crumb structure, middle fertility are selected, the good medium loam of venting capability, grogs 3~5mm of diameter dries standby；
3. by straw rotting fungus waste mushroom leftover, the loam got ready and gypsum according to mass ratio 100: 80:5 ratio is mixed, and is made gap bridge
Caching material；
2）, from granularity quarrel son, in advance with 1% limewash soak 12~15h, by quarrel son and pass a bridge caching expect dry weight compare 70：
30 ratio is got the raw materials ready；
3）, strain make：
1. the caching material moisture content that will pass a bridge is adjusted to 45%, and quarrel for soaking is mixed in proportion, and is filled to the bottle of cleaning
It is interior, highly it is 3/5ths, then the gap bridge caching material 5mm for mixing up moisture is covered, sealed with tampon；
2. sterilizing in pressure cooker is put；
3. sterile working connects straw rotting fungus strain；
4. it is placed in lucifuge, clean culturing room, miscellaneous bacteria is investigated in 25~28 DEG C of cultures at seven days, shake bottle within 20 days, and 35 days long
Full bottle.
The solution have the advantages that：Nutrition needed for caching material of passing a bridge can fully supplement straw rotting fungus mycelial growth is first
Element, and can be passed a bridge by amino acid, reduced sugar and the various active factor necessary to mycelial growth, the 5mm in charge level covering
Caching material promotes mycelium rapid field planting, is colonized than original early 3~4d of formula.Growth is fast, careless containing being adapted in gap bridge caching material
The available nitrogen of rotten bacteria growing, by pass a bridge caching material mix in whole compost, in straw rotting fungus growth course can fast-growth,
Later stage shakes bottle measure and allows upper half bottle to cover with Mycelium culture material and be covered with full bottle multiple spot and grows.The gap bridge caching material that this method is used can
Be not in move back bacterium phenomenon to allow mycelium normally to preserve wherein, meet because the production schedule is changed, strain can not be long-term
The inconvenience that preservation, degeneration are caused.
Specific embodiment
1. caching material of passing a bridge is prepared
Straw rotting fungus mycelium field planting on grain compost is slow, growth is slow, and strain aging is fast, be difficult preservation and transport, makes in the past
" Mycelium in Agaricus blazei Murill is colonized slow, growth is slow on grain compost " can be solved the problems, such as gap bridge material with excrement grass fermentation material,
But never solve the problems, such as " grain strain aging is fast, be difficult preservation and transport ".Using caching material of passing a bridge, can effectively solve
Certainly above-mentioned two problems.
1. straw rotting fungus waste mushroom leftover
There are amino acid, the reduced sugar of suitable straw rotting fungus bacterial strain field planting in waste mushroom leftover after excrement grass fermentation material cultivation straw rotting fungus, it is special
It is not sprouting and field planting that available nitrogen and reduced sugar are conducive to straw rotting fungus bacterial strain.Straw rotting fungus waste mushroom leftover is dried, powder into 5~
10mm is standby.
2. loam
From crumb structure, middle fertility, the good medium loam of venting capability, 3~5mm of grogs diameter.Dry standby.
3. by straw rotting fungus waste mushroom leftover, the loam got ready and gypsum according to 100: 80:5（Mass ratio）Ratio mix, into
It is caching material of passing a bridge.
4. by quarrel and caching material 70 of passing a bridge：30（Dry weight ratio）Ratio get the raw materials ready.
2. be cut son
Granularity quarrel from（3mm±0.5mm）, 12~15h is soaked with 1% limewash in advance, to without white core.
3. strain makes
1. added water using the preceding caching material that will pass a bridge, moisture content is adjusted to 45%, will mix up the gap bridge caching material and the quarrel for soaking of moisture
Son is mixed in proportion, and is filled in the saline bottle of cleaning, is highly 3/5ths, and moisture is mixed up in covering above material
Pass a bridge caching material 5mm, is sealed with tampon, is put into Turnround basket.
2. the Turnround basket that seed bottle will be piled is placed in pressure cooker, has closed pot, boosts to the 0.05 cold sky of MPa emptyings
Gas, continues increasing temperature and pressure, and when pressure reaches 0.11MPa, temperature is incubated 2h when reaching 121 DEG C, waits pressure to be down to 0.05 MPa and puts
Gas.
3. 30 DEG C are cooled to after sterilizing and connect straw rotting fungus strain by sterile working code.
4. it is placed in lucifuge, clean culturing room, miscellaneous bacteria is investigated in 25~28 DEG C of cultures at seven days, shake bottle, 35 within 20 days
It covers with bottle.
4. preservation
Stored under the conditions of 10 DEG C~15 DEG C, storage period is no more than 45d.
There is the problem for being colonized the slow growth low and unsuitable preservation of yield rate, typically based on wheat in straw rotting fungus grain strain
Strain at least needs more than five days from sprouting to being colonized, and has 10%~20% strain not material feeding to be colonized, and finally results in yield rate
Than relatively low；Easily degenerated on wheat culture medium because of mycelia with the good strain of duration, it is aging, cause the unsuitable preservation of strain.In order to
Field planting and preservation problem are solved, we have used gap bridge caching material in strain manufacturing process, and carbon first is to constitute straw rotting fungus
The skeleton of mycelial cell and metabolite, is also the main energy sources needed for the metabolism of straw rotting fungus mycelium, small molecule
Structure is easily absorbed by straw rotting fungus mycelium, and nitrogen provides the original of synthetic cell metabolism product for straw rotting fungus mycelium
Material, selection quick-acting nitrogen source is conducive to straw rotting fungus mycelium to absorb.Secondly.All kinds of carbon sources of our repetition tests and
Nitrogen source, natural materials may possess other factors for being conducive to straw rotting fungus mycelial growth, cause natural materials to compare synthetic
Effect is good.By validation trial, the waste mushroom leftover after cultivation straw rotting fungus, with remarkable result, its straw rotting fungus strain are chosen
2~3 days are only needed from sprouting to being colonized, and 100% strain material feeding is colonized.Straw rotting fungus mycelium is in all of grain culture medium
On cover with after all occur degenerating quickly, aging phenomenon, it may be possible to quick-acting carbon sources decompose too fast in the carbon of grain, and later stage carbon source is not
Foot is caused.Therefore, we add all kinds of raw materials in the medium, find in soil straw rotting fungus mycelium can with long-term surviving,
Sandy soil, loam, turfy soil, Baijiang soil are chosen, it is found that its straw rotting fungus mycelium growing way and biomass are preferable in loam, it may be possible to
Because physical arrangement of the loam in granule, beneficial to water conservation and the certain permeability of holding, it is adapted to the survival of straw rotting fungus mycelium, while
The middle fertility of loam, you can meet straw rotting fungus mycelium metabolism needed for, again will not overnutrition cause mycelium excessive growth.
Grain and caching material of passing a bridge are 7：3 ratios, can both meet mycelium fast-germination, field planting, the requirement of growth, can allow bacterium again
Kind possess preservation term long, adapt to straw rotting fungus Production requirement.
Claims (1)
1. straw rotting fungus is cut sub- cultigen preparation method, it is characterised in that comprise the following steps：
1）, prepare pass a bridge caching material：
1. straw rotting fungus waste mushroom leftover is dried, powder is standby into 5~10mm；
2. crumb structure, middle fertility are selected, the good medium loam of venting capability, grogs 3~5mm of diameter dries standby；
3. by straw rotting fungus waste mushroom leftover, the loam got ready and gypsum according to mass ratio 100: 80:5 ratio is mixed, and is made gap bridge
Caching material；
2）, from granularity quarrel son, in advance with 1% limewash soak 12~15h, by quarrel son and pass a bridge caching expect dry weight compare 70：
30 ratio is got the raw materials ready；
3）, strain make：
1. the caching material moisture content that will pass a bridge is adjusted to 45%, and quarrel for soaking is mixed in proportion, and is filled to the bottle of cleaning
It is interior, highly it is 3/5ths, then the gap bridge caching material 5mm for mixing up moisture is covered, sealed with tampon；
2. sterilizing in pressure cooker is put；
3. sterile working connects straw rotting fungus strain；
4. it is placed in lucifuge, clean culturing room, miscellaneous bacteria is investigated in 25~28 DEG C of cultures at seven days, shake bottle within 20 days, and 35 days long
Full bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611120011.9A CN106818204A (en) | 2016-12-08 | 2016-12-08 | The sub- cultigen preparation method of straw rotting fungus quarrel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611120011.9A CN106818204A (en) | 2016-12-08 | 2016-12-08 | The sub- cultigen preparation method of straw rotting fungus quarrel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106818204A true CN106818204A (en) | 2017-06-13 |
Family
ID=59139999
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611120011.9A Pending CN106818204A (en) | 2016-12-08 | 2016-12-08 | The sub- cultigen preparation method of straw rotting fungus quarrel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106818204A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897273A (en) * | 2010-07-19 | 2010-12-01 | 青岛农业大学 | Coprinus comatus cultivating method and cultivating medium |
| CN103342591A (en) * | 2013-06-30 | 2013-10-09 | 邬金飞 | Straw mushroom cultivated species culture material compatibility, and preparation method of culture material |
| KR20140046515A (en) * | 2012-10-04 | 2014-04-21 | 신필교 | Manufacturing process of lentinus edodes culture |
| CN105875197A (en) * | 2016-04-28 | 2016-08-24 | 江苏沿江地区农业科学研究所 | Agaricus bisporus cultivation method |
-
2016
- 2016-12-08 CN CN201611120011.9A patent/CN106818204A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897273A (en) * | 2010-07-19 | 2010-12-01 | 青岛农业大学 | Coprinus comatus cultivating method and cultivating medium |
| KR20140046515A (en) * | 2012-10-04 | 2014-04-21 | 신필교 | Manufacturing process of lentinus edodes culture |
| CN103342591A (en) * | 2013-06-30 | 2013-10-09 | 邬金飞 | Straw mushroom cultivated species culture material compatibility, and preparation method of culture material |
| CN105875197A (en) * | 2016-04-28 | 2016-08-24 | 江苏沿江地区农业科学研究所 | Agaricus bisporus cultivation method |
Non-Patent Citations (3)
| Title |
|---|
| 向华: "《草菇 金针菇 猴头菌》", 31 January 1999, 中国农业出版社 * |
| 李杨瑞主编: "《食用菌栽培新技术》", 31 December 2006, 广西科学技术出版社 * |
| 杨国亮等: "《草菇无公害生产技术》", 31 January 2003, 中国农业出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104982252B (en) | A method of preparing vegetable seedling substrate by agricultural wastes | |
| CN101606471B (en) | Seeding medium made of vinasse methane-sludge and preparation method thereof | |
| CN104725085A (en) | Solanaceous vegetable seedling medium prepared by taking vegetable waste compost as fertilizer source and preparation method of solanaceous vegetable seedling medium | |
| CN104285678A (en) | Super-high-yield oyster mushroom culture method | |
| CN102643120A (en) | Preparation method of organic fertilizer | |
| CN104987156B (en) | A kind of method of binwang mushroom culture medium and cultivation binwang mushroom using mushroom bran | |
| CN103348868B (en) | Method for cultivating pleurotus cornucopiae by using biogas residues | |
| CN104261917A (en) | Method for preparing edible mushroom waste bud organic fertilizer through rapid fermenting | |
| CN104761309A (en) | Active probiotic organic fertilizer and application thereof | |
| CN104206286B (en) | Scaled cattle farms cow dung cascaded utilization of energy combines method of disposal | |
| CN105152707A (en) | Fermentation raw materials of vegetable seedling substrate and rapid cultivating technology of vegetable seedling substrate | |
| CN102452862A (en) | Organic putrilage controlled-spread fertilizer and preparation method thereof | |
| CN104094772B (en) | Utilize manioc waste, mulberry bar and straw to produce the method for Ji mushroom | |
| CN109197381A (en) | A kind of method of solid spawn liquefaction plantation seafood mushroom | |
| CN104106374B (en) | Utilize bagasse, mulberry bar and maize pulp to produce the method for Ji mushroom | |
| KR101262255B1 (en) | Manufacture method of culture medium for mushroom cultivation containing spent mushroom substrates and composted food | |
| CN101139223A (en) | Amino acid flavouring fertilizer | |
| CN107285863A (en) | Soil-less culturing nutrient block based on biogas residue and mushroom bran and preparation method thereof | |
| CN104671920A (en) | Organic substrate special for chilies and preparation method of organic substrate | |
| CN105884464A (en) | Edible fungus cultivation matrix and manufacturing method thereof | |
| CN101514124A (en) | Coprinus comatus fermentation material and a preparation method thereof | |
| CN101423426B (en) | Organic fertilizer for tobacco and preparation method thereof | |
| CN104446757A (en) | Light medium containing fermented sawdust and preparation method of light medium | |
| CN104285674B (en) | A kind of method utilizing mesh bag wall-cultivating straw mushroom | |
| CN110857261A (en) | Preparation method of edible fungus residue biological organic fertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170613 |
|
| RJ01 | Rejection of invention patent application after publication |