CN106793760B - 病毒抗性烟草及其制备方法 - Google Patents

病毒抗性烟草及其制备方法 Download PDF

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CN106793760B
CN106793760B CN201580035068.6A CN201580035068A CN106793760B CN 106793760 B CN106793760 B CN 106793760B CN 201580035068 A CN201580035068 A CN 201580035068A CN 106793760 B CN106793760 B CN 106793760B
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substitution
eif
virus
gene
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CN106793760A (zh
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高仓由光
古贺一治
新城亮
宇田川久史
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Japan Tobacco Inc
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CN201580035068.6A 2014-06-27 2015-06-29 病毒抗性烟草及其制备方法 Active CN106793760B (zh)

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JP2014133378 2014-06-27
JP2014-133378 2014-06-27
JP2014194424 2014-09-24
JP2014-194424 2014-09-24
PCT/JP2015/068713 WO2015199242A1 (ja) 2014-06-27 2015-06-29 ウイルス抵抗性タバコおよびその作出方法

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EP (1) EP3162195A4 (pt-PT)
JP (1) JP6445006B2 (pt-PT)
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AP (1) AP2016009666A0 (pt-PT)
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Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AP2016009666A0 (pt-PT) 2014-06-27 2016-12-31 Japan Tobacco Inc
WO2017188321A1 (ja) * 2016-04-28 2017-11-02 日本たばこ産業株式会社 ウイルス抵抗性タバコおよびその作出方法
JP6826602B2 (ja) * 2016-08-22 2021-02-03 日本たばこ産業株式会社 乾燥たばこ材料の生産方法
WO2018038249A1 (ja) * 2016-08-26 2018-03-01 日本たばこ産業株式会社 ウイルス抵抗性タバコおよびその作出方法
AU2018400836B2 (en) * 2018-01-09 2021-05-27 Nec Corporation Confirmation apparatus, confirmation method, and program
US11814639B2 (en) * 2018-02-02 2023-11-14 Kikkoman Corporation Virus-resistant plant and method for producing the same
CN110857439B (zh) * 2018-08-20 2022-05-17 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 一种高效产生siRNA的马铃薯Y病毒基因片段、弱毒疫苗、制备方法及其应用
CN110857438B (zh) * 2018-08-20 2022-05-17 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 一种高效产生siRNA的烟草花叶病毒基因片段、弱毒疫苗、制备方法及其应用
CN110857437A (zh) * 2018-08-20 2020-03-03 山东农业大学 一种马铃薯y病毒弱毒株、载体、制备方法及其应用
CN110885796B (zh) * 2018-08-20 2022-04-08 山东农业大学 一种抗马铃薯x病毒的弱毒疫苗、制备方法及其应用
CN109371056B (zh) * 2018-09-16 2022-04-08 云南省烟草农业科学研究院 一种选育对辣椒脉斑驳病毒抗性的烟草植物的方法
CN109082430A (zh) * 2018-09-16 2018-12-25 云南省烟草农业科学研究院 隐性抗烟草脉带花叶病毒的烟草eIFiso4E-S基因及其应用
CN111393516B (zh) * 2020-04-13 2022-04-08 云南省烟草农业科学研究院 一种烟草eIFiso4E-T突变体及在培育抗病毒病烟草中的应用
CN112094942B (zh) * 2020-10-23 2023-09-12 贵州省烟草科学研究院 与烟草pvy抗性紧密连锁的分子标记、引物及应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005118850A1 (fr) * 2004-05-25 2005-12-15 Genoplante-Valor Procede de selection ou d'obtention de plantes resistantes au pvmv.
CN101397569A (zh) * 2008-11-18 2009-04-01 北京师范大学 一种提高烟草病毒抗性的方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003299666A1 (en) 2002-12-17 2004-07-22 Cornell Research Foundation, Inc. RECESSIVE PLANT VIRAL RESISTANCE RESULTS FROM MUTATIONS IN TRANSLATION INITIATION FACTOR eIF4E
WO2008001414A1 (fr) 2006-06-23 2008-01-03 Japan Tobacco Inc. Vecteur cosmide destiné à transformer une plante et son procédé d'utilisation
ES2316298B1 (es) * 2007-09-21 2010-02-16 Consejo Superior De Investigaciones Cientificas Manipulacion en la funcion de atdbp1 para generar resistencia frente a potyvirus.
MX2012000009A (es) 2009-06-30 2012-06-12 Sca Hygiene Prod Ab Sistema de material de limpieza y metodos.
EP2537929B1 (en) 2010-02-17 2017-04-12 Japan Tobacco, Inc. Plant component regulation factor, and use thereof
GB201010740D0 (en) 2010-06-25 2010-08-11 Univ Warwick A plant
AP2016009666A0 (pt-PT) 2014-06-27 2016-12-31 Japan Tobacco Inc

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005118850A1 (fr) * 2004-05-25 2005-12-15 Genoplante-Valor Procede de selection ou d'obtention de plantes resistantes au pvmv.
CN101397569A (zh) * 2008-11-18 2009-04-01 北京师范大学 一种提高烟草病毒抗性的方法

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
A single Amino Acid Change in Viral Genome-Associatede Protein of Potato Virus Y Correlates with Resistance Breaking in ‘Virgin A mutant’Tabacco;Chikara Masuta等;《Phytopathology》;19990201;第89卷(第2期);摘要 *
Characterization of PVY (Potato Virus Y) resistance in tobacco: potential role of an eIF4E gene identified by high throughput sequencing technologies.;E. Julio等;《International Plant & Animal Genome Conference XXI》;20131231;第2-13页 *
Complete nucleotide sequence and genome organization of a Chinese isolate of tobacco bushy top virus;X.H.Mo等;《Achives of Virology》;20030201;第148卷(第2期);摘要 *
Exploring Natural Variations in eIF4E and Screening for Potyviral Resistance in Diverse Nicotiana Species;Jungsu Jung等;《Hort. Environ. Biotechnol》;20131112;第54卷(第5期);摘要、第431页左栏第2段、第438页右栏最后1段至第439页左栏第1段 *
Helper Component Proteinase of the Genus Potyvirus Is an Interaction Partner of Translation Initiation Factors eIF(iso)4E and eIF4E and Contains a 4E Binding Motif;Marjo Ala-Poikela等;《JOURNAL OF VIROLOGY》;20110731;第85卷(第13期);摘要 *
Potyviral VPg and HC-Pro Proteins and the Cellular Translation Initiation Factor eIF(iso)4E Interact with Exoribonuclease Rrp6 and a Small α-Heat Shock Protein;Miguel Angel Freire;《Plant Mol Biol Rep》;20131015;第32卷(第2期);596-604 *
Silencing of the Host Factor eIF(iso)4E Gene Confers Plum Pox Virus Resistance in Plum;Xinhua Wang等;《PLOS ONE》;20130131;第8卷(第1期);第1-12页 *
烟草重要基因篇:1. 烟草抗病相关基因;龚达平;《中国烟草科学》;20140228;第35卷(第1期);第134页第6节 *
烟草马铃薯Y病毒病相关基因eIF4E 的片段克隆及RNAi载体构建;皮金鹏等;《中国农学通报》;20120625;第28卷(第18期);摘要 *

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JP6445006B2 (ja) 2018-12-26
AP2016009666A0 (pt-PT) 2016-12-31
CN106793760A (zh) 2017-05-31
US10400251B2 (en) 2019-09-03
JPWO2015199242A1 (ja) 2017-05-25
EP3162195A1 (en) 2017-05-03
US20170107533A1 (en) 2017-04-20
EP3162195A4 (en) 2017-12-20
WO2015199242A1 (ja) 2015-12-30

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