CN106793760B - 病毒抗性烟草及其制备方法 - Google Patents
病毒抗性烟草及其制备方法 Download PDFInfo
- Publication number
- CN106793760B CN106793760B CN201580035068.6A CN201580035068A CN106793760B CN 106793760 B CN106793760 B CN 106793760B CN 201580035068 A CN201580035068 A CN 201580035068A CN 106793760 B CN106793760 B CN 106793760B
- Authority
- CN
- China
- Prior art keywords
- iso
- eif
- gene
- replaced
- virus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/12—Leaves
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
- A01H1/122—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- A01H1/1245—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance
- A01H1/126—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance for virus resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physiology (AREA)
- Mycology (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014133378 | 2014-06-27 | ||
| JP2014-133378 | 2014-06-27 | ||
| JP2014-194424 | 2014-09-24 | ||
| JP2014194424 | 2014-09-24 | ||
| PCT/JP2015/068713 WO2015199242A1 (ja) | 2014-06-27 | 2015-06-29 | ウイルス抵抗性タバコおよびその作出方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106793760A CN106793760A (zh) | 2017-05-31 |
| CN106793760B true CN106793760B (zh) | 2021-03-09 |
Family
ID=54938317
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201580035068.6A Expired - Fee Related CN106793760B (zh) | 2014-06-27 | 2015-06-29 | 病毒抗性烟草及其制备方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US10400251B2 (enExample) |
| EP (1) | EP3162195A4 (enExample) |
| JP (1) | JP6445006B2 (enExample) |
| CN (1) | CN106793760B (enExample) |
| AP (1) | AP2016009666A0 (enExample) |
| WO (1) | WO2015199242A1 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106793760B (zh) | 2014-06-27 | 2021-03-09 | 日本烟草产业株式会社 | 病毒抗性烟草及其制备方法 |
| WO2017188321A1 (ja) * | 2016-04-28 | 2017-11-02 | 日本たばこ産業株式会社 | ウイルス抵抗性タバコおよびその作出方法 |
| CN109640707B (zh) * | 2016-08-22 | 2022-03-08 | 日本烟草产业株式会社 | 干燥烟草材料的生产方法 |
| BR112019003785A2 (pt) * | 2016-08-26 | 2019-05-21 | Japan Tobacco, Inc. | tabaco resistente a vírus e método de melhoramento para o mesmo |
| AU2018400836B2 (en) * | 2018-01-09 | 2021-05-27 | Nec Corporation | Confirmation apparatus, confirmation method, and program |
| EP3760037A4 (en) * | 2018-02-02 | 2021-12-08 | Kikkoman Corporation | VIRUS RESISTANT PLANT AND PROCESS FOR PRODUCING THE SAME |
| CN110857439B (zh) * | 2018-08-20 | 2022-05-17 | 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 | 一种高效产生siRNA的马铃薯Y病毒基因片段、弱毒疫苗、制备方法及其应用 |
| CN110885796B (zh) * | 2018-08-20 | 2022-04-08 | 山东农业大学 | 一种抗马铃薯x病毒的弱毒疫苗、制备方法及其应用 |
| CN110857437A (zh) * | 2018-08-20 | 2020-03-03 | 山东农业大学 | 一种马铃薯y病毒弱毒株、载体、制备方法及其应用 |
| CN110857438B (zh) * | 2018-08-20 | 2022-05-17 | 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 | 一种高效产生siRNA的烟草花叶病毒基因片段、弱毒疫苗、制备方法及其应用 |
| CN109082430A (zh) * | 2018-09-16 | 2018-12-25 | 云南省烟草农业科学研究院 | 隐性抗烟草脉带花叶病毒的烟草eIFiso4E-S基因及其应用 |
| CN109371056B (zh) * | 2018-09-16 | 2022-04-08 | 云南省烟草农业科学研究院 | 一种选育对辣椒脉斑驳病毒抗性的烟草植物的方法 |
| CN111393516B (zh) * | 2020-04-13 | 2022-04-08 | 云南省烟草农业科学研究院 | 一种烟草eIFiso4E-T突变体及在培育抗病毒病烟草中的应用 |
| CN112094942B (zh) * | 2020-10-23 | 2023-09-12 | 贵州省烟草科学研究院 | 与烟草pvy抗性紧密连锁的分子标记、引物及应用 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005118850A1 (fr) * | 2004-05-25 | 2005-12-15 | Genoplante-Valor | Procede de selection ou d'obtention de plantes resistantes au pvmv. |
| CN101397569A (zh) * | 2008-11-18 | 2009-04-01 | 北京师范大学 | 一种提高烟草病毒抗性的方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004057941A2 (en) | 2002-12-17 | 2004-07-15 | Cornell Research Foundation, Inc. | RECESSIVE PLANT VIRAL RESISTANCE RESULTS FROM MUTATIONS IN TRANSLATION INITIATION FACTOR eIF4E |
| WO2008001414A1 (en) | 2006-06-23 | 2008-01-03 | Japan Tobacco Inc. | Cosmid vector for transforming plant and method of using the same |
| ES2316298B1 (es) * | 2007-09-21 | 2010-02-16 | Consejo Superior De Investigaciones Cientificas | Manipulacion en la funcion de atdbp1 para generar resistencia frente a potyvirus. |
| US20120104016A1 (en) | 2009-06-30 | 2012-05-03 | Sca Hygiene Products Ab | Wiping material system and methods |
| EP2537929B1 (en) | 2010-02-17 | 2017-04-12 | Japan Tobacco, Inc. | Plant component regulation factor, and use thereof |
| GB201010740D0 (en) | 2010-06-25 | 2010-08-11 | Univ Warwick | A plant |
| CN106793760B (zh) | 2014-06-27 | 2021-03-09 | 日本烟草产业株式会社 | 病毒抗性烟草及其制备方法 |
-
2015
- 2015-06-29 CN CN201580035068.6A patent/CN106793760B/zh not_active Expired - Fee Related
- 2015-06-29 WO PCT/JP2015/068713 patent/WO2015199242A1/ja not_active Ceased
- 2015-06-29 AP AP2016009666A patent/AP2016009666A0/en unknown
- 2015-06-29 JP JP2016529686A patent/JP6445006B2/ja not_active Expired - Fee Related
- 2015-06-29 EP EP15811561.8A patent/EP3162195A4/en not_active Ceased
-
2016
- 2016-12-21 US US15/387,182 patent/US10400251B2/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005118850A1 (fr) * | 2004-05-25 | 2005-12-15 | Genoplante-Valor | Procede de selection ou d'obtention de plantes resistantes au pvmv. |
| CN101397569A (zh) * | 2008-11-18 | 2009-04-01 | 北京师范大学 | 一种提高烟草病毒抗性的方法 |
Non-Patent Citations (9)
| Title |
|---|
| A single Amino Acid Change in Viral Genome-Associatede Protein of Potato Virus Y Correlates with Resistance Breaking in ‘Virgin A mutant’Tabacco;Chikara Masuta等;《Phytopathology》;19990201;第89卷(第2期);摘要 * |
| Characterization of PVY (Potato Virus Y) resistance in tobacco: potential role of an eIF4E gene identified by high throughput sequencing technologies.;E. Julio等;《International Plant & Animal Genome Conference XXI》;20131231;第2-13页 * |
| Complete nucleotide sequence and genome organization of a Chinese isolate of tobacco bushy top virus;X.H.Mo等;《Achives of Virology》;20030201;第148卷(第2期);摘要 * |
| Exploring Natural Variations in eIF4E and Screening for Potyviral Resistance in Diverse Nicotiana Species;Jungsu Jung等;《Hort. Environ. Biotechnol》;20131112;第54卷(第5期);摘要、第431页左栏第2段、第438页右栏最后1段至第439页左栏第1段 * |
| Helper Component Proteinase of the Genus Potyvirus Is an Interaction Partner of Translation Initiation Factors eIF(iso)4E and eIF4E and Contains a 4E Binding Motif;Marjo Ala-Poikela等;《JOURNAL OF VIROLOGY》;20110731;第85卷(第13期);摘要 * |
| Potyviral VPg and HC-Pro Proteins and the Cellular Translation Initiation Factor eIF(iso)4E Interact with Exoribonuclease Rrp6 and a Small α-Heat Shock Protein;Miguel Angel Freire;《Plant Mol Biol Rep》;20131015;第32卷(第2期);596-604 * |
| Silencing of the Host Factor eIF(iso)4E Gene Confers Plum Pox Virus Resistance in Plum;Xinhua Wang等;《PLOS ONE》;20130131;第8卷(第1期);第1-12页 * |
| 烟草重要基因篇:1. 烟草抗病相关基因;龚达平;《中国烟草科学》;20140228;第35卷(第1期);第134页第6节 * |
| 烟草马铃薯Y病毒病相关基因eIF4E 的片段克隆及RNAi载体构建;皮金鹏等;《中国农学通报》;20120625;第28卷(第18期);摘要 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JPWO2015199242A1 (ja) | 2017-05-25 |
| US10400251B2 (en) | 2019-09-03 |
| EP3162195A1 (en) | 2017-05-03 |
| CN106793760A (zh) | 2017-05-31 |
| BR112016030279A2 (pt) | 2017-11-14 |
| EP3162195A4 (en) | 2017-12-20 |
| AP2016009666A0 (enExample) | 2016-12-31 |
| US20170107533A1 (en) | 2017-04-20 |
| JP6445006B2 (ja) | 2018-12-26 |
| WO2015199242A1 (ja) | 2015-12-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106793760B (zh) | 病毒抗性烟草及其制备方法 | |
| US11659807B2 (en) | Virus-resistant tobacco and breeding method therefor | |
| WO2019038417A1 (en) | METHODS FOR INCREASING GRAIN YIELD | |
| US20180273972A1 (en) | Methods of increasing virus resistance in cucumber using genome editing and plants generated thereby | |
| IL313136A (en) | Plants with tolerance or resistance to ToBRFV and methods for their production | |
| CN110892074A (zh) | 用于增加香蕉的保质期的组成物及方法 | |
| CN115873824B (zh) | 水稻rsb11基因在抗纹枯病中的应用 | |
| US20220106607A1 (en) | Gene for parthenogenesis | |
| CN113677696A (zh) | 一种培育抗灰斑病植物的方法 | |
| CN115485386B (zh) | 低生物碱含量的烟草属植物体及其制备方法 | |
| WO2017188321A1 (ja) | ウイルス抵抗性タバコおよびその作出方法 | |
| KR20220139958A (ko) | 토바모바이러스 저항성 토마토 식물, 토바모바이러스 저항성 토마토 식물의 생산 방법, 토마토 식물에 있어서의 토바모바이러스 저항성 부여 방법, 토바모바이러스 저항성 토마토 식물의 스크리닝 방법 및 토마토 식물에 있어서의 토바모바이러스 저항성 검출 방법 | |
| WO2020208017A1 (en) | Diagnostic kit and method for sweet-based rice blight resistance and resistant breeding lines | |
| EP3361858A1 (en) | Brassica plants with altered properties in seed production | |
| US20240240195A1 (en) | Compositions and methods for increasing extractibility of solids from coffee beans | |
| JP2023526035A (ja) | 標的突然変異生成によって変異体植物を得るための方法 | |
| JP5750749B2 (ja) | イネの弱勢判定方法 | |
| WO2025052377A1 (en) | Cowpea plants with improved traits and methods for generating the same | |
| JP2023111899A (ja) | 短葯形質を有するイネ、及びその製造方法 | |
| BR112016030279B1 (pt) | Métodos para reproduzir e selecionar tabaco resistente a vírus, polinucleotídeo para uso em detecção e marcador de dna para avaliar tabaco | |
| 조영득 | Molecular Genetic Analysis of Cytoplasmic Male Sterility by Mitochondrial Genome Sequencing and Isolation of a Restorer-of-fertility Candidate Gene in Pepper (Capsicum annuum L.) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210309 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |