CN106749508A - The extracting method of high-purity Radix Isatidis vegetable protein - Google Patents
The extracting method of high-purity Radix Isatidis vegetable protein Download PDFInfo
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- CN106749508A CN106749508A CN201611143031.8A CN201611143031A CN106749508A CN 106749508 A CN106749508 A CN 106749508A CN 201611143031 A CN201611143031 A CN 201611143031A CN 106749508 A CN106749508 A CN 106749508A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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Abstract
The invention discloses a kind of extracting method of high-purity Radix Isatidis vegetable protein, including：Step one, chromatogram of Radix Isatidis is crushed；Step 2, to adding water in banlangen granules, banlangen keli, immersion 5~6 hours, in immersion process, within 4 hours above, control water temperature is maintained at 40~45 DEG C, within remaining time, control water temperature is maintained at 50 DEG C, centrifugal treating 3~5 minutes under 3000~4000rpm, are then supplemented with water afterwards, are placed in centrifugal treating 20~25 minutes under 4500~5500rpm again afterwards；Step 3, supernatant is taken, ultrafiltration takes filtrate, and freeze-drying obtains crude protein；Step 4, crude protein is dissolved, regulation to pH value is 4~6, obtains crude protein solution；Step 5, with cationic ion-exchange resin to treatment crude protein solution, obtain refined Radix Isatidis vegetable protein.The present invention improves the extraction efficiency of vegetable protein, helps to expand its application in anti-virus aspect.
Description
Technical field
The present invention relates to a kind of extracting method of high-purity Radix Isatidis vegetable protein.
Background technology
In recent years, SARS, bird flu and H1N1virus frequently break out in China or even world wide, right
The life security of people and social economy cause greatly infringement.
For a long time, people and achieve good result by vaccine come pre- anti-virus, but vaccine only has and virus subtype
Just can be effective during matching.Virus variation is fast and outburst has unpredictability, and vaccine research and production relatively lag behind.
For this drawback of vaccine, people are started widely studied antiviral drugs, are come to antiviral with this.Western medicine is disease-resistant
Cytotoxic drug has the features such as instant effect, curative effect are high, effect is accurate, but is respectively provided with specific aim per class medicine, specific just for certain
Virus work, because of the easy variability factor of viral antigen, cause some to make the application of the chemicals of antiviral drugs
There is certain limitation.The effects such as many Chinese medicines have suppressing virus replication, prevent pathological changes caused by virus, Chinese medicine is exempted from by regulation
The comprehensive functions such as epidemic disease function, improvement pulmonary circulation, antalgic and inflammation relieving, and then suppress or kill virus, with good preventing and treating virus
Effect.
It is also one of focus of research in recent years fully to excavate and find antiviral active substance from traditional Chinese medicine.Radix Isatidis
It is the standing medicine of family with reliable antivirus action.Radix Isatidis is cruciferae isatis (Isatis
Indigotica dry root), the effect of with clearing heat and detoxicating, cool blood relieving sore-throat, is usually used in treatment influenza, parotitis, warm disease hair
The diseases of viral infection such as heat, Japanese Type-B encephalitis, hepatitis.Radix Isatidis complicated component, its material base and mechanism of action
Indefinite, production technology is still relatively backward.Accordingly, it would be desirable to the vegetable protein with antiviral effectiveness in Radix Isatidis is carried
Taking technique is further studied, to expand the application to the vegetable protein in Radix Isatidis.
The content of the invention
For above-mentioned technical problem, the present invention has designed and developed a kind of extraction efficiency high-purity Radix Isatidis plant egg higher
White extracting method.
The present invention provide technical scheme be：
A kind of extracting method of high-purity Radix Isatidis vegetable protein, including：
Step one, chromatogram of Radix Isatidis is ground into the banlangen granules, banlangen keli that particle diameter is 300~350 μm；
Step 2, to adding water in banlangen granules, banlangen keli, the addition of water is 10~15 times of banlangen granules, banlangen keli, 50~60
Soaked 5~6 hours at DEG C, in immersion process, within 4 hours above, control water temperature is maintained at 40~45 DEG C, in residue
In time, control water temperature is maintained at 50 DEG C, and centrifugal treating 3~5 minutes under 3000~4000rpm, are then supplemented with water afterwards,
The water of supplement is 2~3 times of banlangen granules, banlangen keli, is placed in centrifugal treating 20~25 minutes under 4500~5500rpm again afterwards；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and freeze-drying obtains crude protein；
Step 4, by crude protein water dissolves, regulation to pH value is 4~6, obtains crude protein solution；
Step 5, with cationic ion-exchange resin to treatment crude protein solution, eluted repeatedly with water, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Preferably, in the extracting method of described high-purity Radix Isatidis vegetable protein, in the step one, by Radix Isatidis
Pulverizing medicinal materials are into the banlangen granules, banlangen keli that particle diameter is 310~320 μm.
Preferably, in the extracting method of described high-purity Radix Isatidis vegetable protein, in the step 2, to Radix Isatidis
Water is added in particle, the addition of water is 10 times of banlangen granules, banlangen keli, is soaked 5~6 hours, in immersion process, in 4 above
Within hour, control water temperature is maintained at 40~45 DEG C, and within remaining time, control water temperature is maintained at 50 DEG C, afterwards 3000~
Centrifugal treating 3~5 minutes under 4000rpm, are then supplemented with water, and the water of supplement is 2 times of banlangen granules, banlangen keli, is placed in again afterwards
Centrifugal treating 20~25 minutes under 4500~5500rpm.
Preferably, in the extracting method of described high-purity Radix Isatidis vegetable protein, in the step 2, to Radix Isatidis
Water is added in particle, the addition of water is 10 times of banlangen granules, banlangen keli, is soaked 5 hours, small in 4 above in immersion process
When within, control water temperature be maintained at 40 DEG C, within remaining time, control water temperature be maintained at 50 DEG C, afterwards in 3000~4000rpm
Lower centrifugal treating 3~5 minutes, is then supplemented with water, and the water of supplement is 2 times of banlangen granules, banlangen keli, it is placed in 4500 again afterwards~
Centrifugal treating 20~25 minutes under 5500rpm.
Preferably, in the extracting method of described high-purity Radix Isatidis vegetable protein, in the step 2, to Radix Isatidis
Water is added in particle, the addition of water is 10 times of banlangen granules, banlangen keli, is soaked 5 hours, small in 4 above in immersion process
When within, control water temperature be maintained at 40 DEG C, within remaining time, control water temperature be maintained at 50 DEG C, be centrifuged at 3,000 rpm afterwards
Treatment 3 minutes, is then supplemented with water, and the water of supplement is 2 times of banlangen granules, banlangen keli, is placed in again afterwards under 4500rpm at centrifugation
Reason 20 minutes.
Preferably, in the extracting method of described high-purity Radix Isatidis vegetable protein, in the step 5, cation is used
Exchanger resin is eluted, then cationic ion-exchange resin is entered with the ammoniacal liquor that pH value is 8~9 repeatedly to treatment crude protein solution with water
Row wash-out, the eluent that water elution and ammoniacal liquor are eluted all is retained, and carries out freeze-drying, obtains refined Radix Isatidis and plants
Thing albumen.
The extracting method of high-purity Radix Isatidis vegetable protein of the present invention is extracted by careful design extraction process
Vegetable protein purification mass percent more than 94%, improve the extraction efficiency of vegetable protein, contribute to expand
Its application in anti-virus aspect.
Specific embodiment
The present invention is described in further detail below, with make those skilled in the art with reference to specification word being capable of evidence
To implement.
The present invention provides a kind of extracting method of high-purity Radix Isatidis vegetable protein, including：
Step one, chromatogram of Radix Isatidis is ground into the banlangen granules, banlangen keli that particle diameter is 300~350 μm；
Step 2, to adding water in banlangen granules, banlangen keli, the addition of water is 10~15 times of banlangen granules, banlangen keli, immersion 5~6
Hour, in immersion process, within 4 hours above, control water temperature is maintained at 40~45 DEG C, within remaining time, control
Water temperature is maintained at 50 DEG C, afterwards centrifugal treating 3~5 minutes under 3000~4000rpm, is then supplemented with water, the water of supplement
It is 2~3 times of banlangen granules, banlangen keli, is placed in centrifugal treating 20~25 minutes under 4500~5500rpm again afterwards；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and freeze-drying obtains crude protein；
Step 4, by crude protein water dissolves, regulation to pH value is 4~6, obtains crude protein solution；
Step 5, with cationic ion-exchange resin to treatment crude protein solution, eluted repeatedly with water, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
The extracting method of high-purity Radix Isatidis vegetable protein of the present invention is extracted by careful design extraction process
Vegetable protein purification mass percent more than 94%, improve the extraction efficiency of vegetable protein, contribute to expand
Its application in anti-virus aspect.
Preferably, in the extracting method of described high-purity Radix Isatidis vegetable protein, in the step one, by Radix Isatidis
Pulverizing medicinal materials are into the banlangen granules, banlangen keli that particle diameter is 310~320 μm.
Preferably, in the extracting method of described high-purity Radix Isatidis vegetable protein, in the step 2, to Radix Isatidis
Water is added in particle, the addition of water is 10 times of banlangen granules, banlangen keli, is soaked 5~6 hours, in immersion process, in 4 above
Within hour, control water temperature is maintained at 40~45 DEG C, and within remaining time, control water temperature is maintained at 50 DEG C, afterwards 3000~
Centrifugal treating 3~5 minutes under 4000rpm, are then supplemented with water, and the water of supplement is 2 times of banlangen granules, banlangen keli, is placed in again afterwards
Centrifugal treating 20~25 minutes under 4500~5500rpm.
Preferably, in the extracting method of described high-purity Radix Isatidis vegetable protein, in the step 2, to Radix Isatidis
Water is added in particle, the addition of water is 10 times of banlangen granules, banlangen keli, is soaked 5 hours, small in 4 above in immersion process
When within, control water temperature be maintained at 40 DEG C, within remaining time, control water temperature be maintained at 50 DEG C, afterwards in 3000~4000rpm
Lower centrifugal treating 3~5 minutes, is then supplemented with water, and the water of supplement is 2 times of banlangen granules, banlangen keli, it is placed in 4500 again afterwards~
Centrifugal treating 20~25 minutes under 5500rpm.
Preferably, in the extracting method of described high-purity Radix Isatidis vegetable protein, in the step 2, to Radix Isatidis
Water is added in particle, the addition of water is 10 times of banlangen granules, banlangen keli, is soaked 5 hours, small in 4 above in immersion process
When within, control water temperature be maintained at 40 DEG C, within remaining time, control water temperature be maintained at 50 DEG C, be centrifuged at 3,000 rpm afterwards
Treatment 3 minutes, is then supplemented with water, and the water of supplement is 2 times of banlangen granules, banlangen keli, is placed in again afterwards under 4500rpm at centrifugation
Reason 20 minutes.
Preferably, in the extracting method of described high-purity Radix Isatidis vegetable protein, in the step 5, cation is used
Exchanger resin is eluted, then cationic ion-exchange resin is entered with the ammoniacal liquor that pH value is 8~9 repeatedly to treatment crude protein solution with water
Row wash-out, the eluent that water elution and ammoniacal liquor are eluted all is retained, and carries out freeze-drying, obtains refined Radix Isatidis and plants
Thing albumen.
Embodiment one
Step one, chromatogram of Radix Isatidis is ground into the banlangen granules, banlangen keli that particle diameter is 300~310 μm；
Step 2, to water is added in banlangen granules, banlangen keli, the addition of water is 10 times of banlangen granules, banlangen keli, is soaked 5 hours,
In immersion process, within 4 hours above, control water temperature is maintained at 40 DEG C, and within remaining time, control water temperature is maintained at 50
DEG C, then centrifugal treating 3 minutes at 3,000 rpm afterwards are supplemented with water, and the water of supplement is 2 times of banlangen granules, banlangen keli, afterwards
Centrifugal treating 20 minutes under 4500rpm are placed in again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and freeze-drying obtains crude protein；
Step 4, by crude protein water dissolves, regulation to pH value is 4, obtains crude protein solution；
Step 5, with cationic ion-exchange resin to treatment crude protein solution, eluted repeatedly with water, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94.5%.
Embodiment two
Step one, chromatogram of Radix Isatidis is ground into the banlangen granules, banlangen keli that particle diameter is 300~320 μm；
Step 2, to water is added in banlangen granules, banlangen keli, the addition of water is 15 times of banlangen granules, banlangen keli, is soaked 6 hours,
In immersion process, within 4 hours above, control water temperature is maintained at 45 DEG C, and within remaining time, control water temperature is maintained at 50
DEG C, centrifugal treating 5 minutes under 4000rpm, are then supplemented with water afterwards, and the water of supplement is 3 times of banlangen granules, banlangen keli, afterwards
Centrifugal treating 25 minutes under 5500rpm are placed in again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and freeze-drying obtains crude protein；
Step 4, by crude protein water dissolves, regulation to pH value is 6, obtains crude protein solution；
Step 5, with cationic ion-exchange resin to treatment crude protein solution, eluted repeatedly with water, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94.7%.
Embodiment three
Step one, chromatogram of Radix Isatidis is ground into the banlangen granules, banlangen keli that particle diameter is 340~350 μm；
Step 2, to water is added in banlangen granules, banlangen keli, the addition of water is 15 times of banlangen granules, banlangen keli, is soaked 6 hours,
In immersion process, within 4 hours above, control water temperature is maintained at 42 DEG C, and within remaining time, control water temperature is maintained at 50
DEG C, centrifugal treating 5 minutes under 4000rpm, are then supplemented with water afterwards, and the water of supplement is 3 times of banlangen granules, banlangen keli, afterwards
Centrifugal treating 25 minutes under 5500rpm are placed in again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and freeze-drying obtains crude protein；
Step 4, by crude protein water dissolves, regulation to pH value is 6, obtains crude protein solution；
Step 5, with cationic ion-exchange resin to treatment crude protein solution, eluted repeatedly with water, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94.9%.
Example IV
Step one, chromatogram of Radix Isatidis is ground into the banlangen granules, banlangen keli that particle diameter is 310~320 μm；
Step 2, to water is added in banlangen granules, banlangen keli, the addition of water is 10 times of banlangen granules, banlangen keli, is soaked 5 hours,
In immersion process, within 4 hours above, control water temperature is maintained at 40 DEG C, and within remaining time, control water temperature is maintained at 50
DEG C, then centrifugal treating 3 minutes at 3,000 rpm afterwards are supplemented with water, and the water of supplement is 2 times of banlangen granules, banlangen keli, afterwards
Centrifugal treating 20 minutes under 4500rpm are placed in again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and freeze-drying obtains crude protein；
Step 4, by crude protein water dissolves, regulation to pH value is 6, obtains crude protein solution；
Step 5, with cationic ion-exchange resin to treatment crude protein solution, eluted repeatedly with water, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94.9%.
Embodiment five
Step one, chromatogram of Radix Isatidis is ground into the banlangen granules, banlangen keli that particle diameter is 310~325 μm；
Step 2, to water is added in banlangen granules, banlangen keli, the addition of water is 10 times of banlangen granules, banlangen keli, is soaked 5.5 hours,
In immersion process, within 4 hours above, control water temperature is maintained at 40 DEG C, and within remaining time, control water temperature is maintained at
50 DEG C, then centrifugal treating 3 minutes at 3,000 rpm afterwards are supplemented with water, and the water of supplement is 2 times of banlangen granules, banlangen keli, it
It is placed in centrifugal treating 20 minutes under 4500rpm again afterwards；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and freeze-drying obtains crude protein；
Step 4, by crude protein water dissolves, regulation to pH value is 6, obtains crude protein solution；
Step 5, with cationic ion-exchange resin to treatment crude protein solution, eluted repeatedly with water, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94.8%.
Embodiment six
Step one, chromatogram of Radix Isatidis is ground into the banlangen granules, banlangen keli that particle diameter is 310~320 μm；
Step 2, to water is added in banlangen granules, banlangen keli, the addition of water is 10 times of banlangen granules, banlangen keli, is soaked 5 hours,
In immersion process, within 4 hours above, control water temperature is maintained at 45 DEG C, and within remaining time, control water temperature is maintained at 50
DEG C, centrifugal treating 3 minutes under 3500rpm, are then supplemented with water afterwards, and the water of supplement is 2 times of banlangen granules, banlangen keli, afterwards
Centrifugal treating 22 minutes under 4500rpm are placed in again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and freeze-drying obtains crude protein；
Step 4, by crude protein water dissolves, regulation to pH value is 6, obtains crude protein solution；
Step 5, with cationic ion-exchange resin to treatment crude protein solution, eluted repeatedly with water, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94.7%.
Embodiment seven
Step one, chromatogram of Radix Isatidis is ground into the banlangen granules, banlangen keli that particle diameter is 310~320 μm；
Step 2, to water is added in banlangen granules, banlangen keli, the addition of water is 10 times of banlangen granules, banlangen keli, is soaked 5 hours,
In immersion process, within 4 hours above, control water temperature is maintained at 45 DEG C, and within remaining time, control water temperature is maintained at 50
DEG C, then centrifugal treating 3 minutes at 3,000 rpm afterwards are supplemented with water, and the water of supplement is 3 times of banlangen granules, banlangen keli, afterwards
Centrifugal treating 20 minutes under 4500rpm are placed in again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and freeze-drying obtains crude protein；
Step 4, by crude protein water dissolves, regulation to pH value is 6, obtains crude protein solution；
Step 5, with cationic ion-exchange resin to treatment crude protein solution, eluted repeatedly with water, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94.7%.
Embodiment eight
Step one, chromatogram of Radix Isatidis is ground into the banlangen granules, banlangen keli that particle diameter is 310~320 μm；
Step 2, to water is added in banlangen granules, banlangen keli, the addition of water is 10 times of banlangen granules, banlangen keli, is soaked 5 hours,
In immersion process, within 4 hours above, control water temperature is maintained at 44 DEG C, and within remaining time, control water temperature is maintained at 50
DEG C, then centrifugal treating 3 minutes at 3,000 rpm afterwards are supplemented with water, and the water of supplement is 2 times of banlangen granules, banlangen keli, afterwards
Centrifugal treating 24 minutes under 4550rpm are placed in again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and freeze-drying obtains crude protein；
Step 4, by crude protein water dissolves, regulation to pH value is 6, obtains crude protein solution；
Step 5, with cationic ion-exchange resin to treatment crude protein solution, eluted repeatedly with water, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94.7%.
Embodiment nine
Step one, chromatogram of Radix Isatidis is ground into the banlangen granules, banlangen keli that particle diameter is 310~320 μm；
Step 2, to water is added in banlangen granules, banlangen keli, the addition of water is 10 times of banlangen granules, banlangen keli, is soaked 6 hours,
In immersion process, within 4 hours above, control water temperature is maintained at 42 DEG C, and within remaining time, control water temperature is maintained at 50
DEG C, then centrifugal treating 3 minutes at 3,000 rpm afterwards are supplemented with water, and the water of supplement is 3 times of banlangen granules, banlangen keli, afterwards
Centrifugal treating 20 minutes under 4500rpm are placed in again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and freeze-drying obtains crude protein；
Step 4, by crude protein water dissolves, regulation to pH value is 6, obtains crude protein solution；
Step 5, with cationic ion-exchange resin to treatment crude protein solution, eluted repeatedly with water, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94.5%.
Embodiment ten
Step one, chromatogram of Radix Isatidis is ground into the banlangen granules, banlangen keli that particle diameter is 320~330 μm；
Step 2, to water is added in banlangen granules, banlangen keli, the addition of water is 10 times of banlangen granules, banlangen keli, is soaked 5 hours,
In immersion process, within 4 hours above, control water temperature is maintained at 43 DEG C, and within remaining time, control water temperature is maintained at 50
DEG C, then centrifugal treating 3 minutes at 3,000 rpm afterwards are supplemented with water, and the water of supplement is 2 times of banlangen granules, banlangen keli, afterwards
Centrifugal treating 20 minutes under 4500rpm are placed in again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and freeze-drying obtains crude protein；
Step 4, by crude protein water dissolves, regulation to pH value is 6, obtains crude protein solution；
Step 5, with cationic ion-exchange resin to treatment crude protein solution, eluted repeatedly with water, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 95%.
Comparative example
Radix Isatidis vegetable protein is extracted using existing process, mass percent of the vegetable protein in purification is only
Account for 87%.
Although embodiment of the present invention is disclosed as above, it is not restricted to listed in specification and implementation method
With, it can be applied to various suitable the field of the invention completely, for those skilled in the art, can be easily
Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, the present invention is not limited
In specific details.
Claims (6)
1. a kind of extracting method of high-purity Radix Isatidis vegetable protein, it is characterised in that including：
Step one, chromatogram of Radix Isatidis is ground into the banlangen granules, banlangen keli that particle diameter is 300~350 μm；
Step 2, to adding water in banlangen granules, banlangen keli, the addition of water is 10~15 times of banlangen granules, banlangen keli, and immersion 5~6 is small
When, in immersion process, within 4 hours above, control water temperature is maintained at 40~45 DEG C, within remaining time, controls water
Temperature is maintained at 50 DEG C, afterwards centrifugal treating 3~5 minutes under 3000~4000rpm, is then supplemented with water, and the water of supplement is
2~3 times of banlangen granules, banlangen keli, are placed in centrifugal treating 20~25 minutes under 4500~5500rpm again afterwards；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and freeze-drying obtains crude protein；
Step 4, by crude protein water dissolves, regulation to pH value is 4~6, obtains crude protein solution；
Step 5, with cationic ion-exchange resin to treatment crude protein solution, eluted repeatedly with water, leave and take eluent freeze-drying,
Obtain refined Radix Isatidis vegetable protein.
2. the extracting method of high-purity Radix Isatidis vegetable protein as claimed in claim 1, it is characterised in that the step one
In, chromatogram of Radix Isatidis is ground into the banlangen granules, banlangen keli that particle diameter is 310~320 μm.
3. the extracting method of high-purity Radix Isatidis vegetable protein as claimed in claim 1, it is characterised in that the step 2
In, to water is added in banlangen granules, banlangen keli, the addition of water is 10 times of banlangen granules, banlangen keli, is soaked 5~6 hours, in immersion process
In, within 4 hours above, control water temperature is maintained at 40~45 DEG C, and within remaining time, control water temperature is maintained at 50 DEG C,
Centrifugal treating 3~5 minutes under 3000~4000rpm, are then supplemented with water afterwards, and the water of supplement is the 2 of banlangen granules, banlangen keli
Times, it is placed in centrifugal treating 20~25 minutes under 4500~5500rpm again afterwards.
4. the extracting method of high-purity Radix Isatidis vegetable protein as claimed in claim 3, it is characterised in that the step 2
In, to water is added in banlangen granules, banlangen keli, the addition of water is 10 times of banlangen granules, banlangen keli, is soaked 5 hours, in immersion process,
Within 4 hours above, control water temperature is maintained at 40 DEG C, and within remaining time, control water temperature is maintained at 50 DEG C, Zhi Hou
Centrifugal treating 3~5 minutes under 3000~4000rpm, are then supplemented with water, and the water of supplement is 2 times of banlangen granules, banlangen keli, afterwards
Centrifugal treating 20~25 minutes under 4500~5500rpm are placed in again.
5. the extracting method of high-purity Radix Isatidis vegetable protein as claimed in claim 4, it is characterised in that the step 2
In, to water is added in banlangen granules, banlangen keli, the addition of water is 10 times of banlangen granules, banlangen keli, is soaked 5 hours, in immersion process,
Within 4 hours above, control water temperature is maintained at 40 DEG C, and within remaining time, control water temperature is maintained at 50 DEG C, Zhi Hou
Centrifugal treating 3 minutes under 3000rpm, are then supplemented with water, and the water of supplement is 2 times of banlangen granules, banlangen keli, is placed in again afterwards
Centrifugal treating 20 minutes under 4500rpm.
6. the extracting method of high-purity Radix Isatidis vegetable protein as claimed in claim 1, it is characterised in that the step 5
In, with cationic ion-exchange resin to treatment crude protein solution, eluted repeatedly with water, then with the ammoniacal liquor that pH value is 8~9 to cation
Exchanger resin is eluted, and the eluent that water elution and ammoniacal liquor are eluted all is retained, and carries out freeze-drying, is refined
Radix Isatidis vegetable protein.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611143031.8A CN106749508A (en) | 2016-12-13 | 2016-12-13 | The extracting method of high-purity Radix Isatidis vegetable protein |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611143031.8A CN106749508A (en) | 2016-12-13 | 2016-12-13 | The extracting method of high-purity Radix Isatidis vegetable protein |
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