CN105726591A - Method for extracting effective components of isatis roots through aqueous enzymatic method - Google Patents
Method for extracting effective components of isatis roots through aqueous enzymatic method Download PDFInfo
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- CN105726591A CN105726591A CN201410755966.6A CN201410755966A CN105726591A CN 105726591 A CN105726591 A CN 105726591A CN 201410755966 A CN201410755966 A CN 201410755966A CN 105726591 A CN105726591 A CN 105726591A
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Abstract
The invention discloses a method for extracting effective components of isatis roots through an aqueous enzymatic method. The method is characterized by comprising the following steps: (1) pulverizing isatis roots and adding water while performing uniform stirring to obtain a thick liquid for later use; (2) adjusting acidity of the thick liquid through citric acid, and adding protease-cellulose compound enzyme for enzymolysis; (3) adding a proper amount of water into the obtained enzymatic hydrolysate, heating the obtained solution to 85-55 DEG C, performing extraction, performing precipitation, and performing press filtration through a filter press to obtain a filtrate and filter residue; and (4) adding a phosphoric acid solution with a concentration of 85% into the filtrate, performing full mixing, adding a proper amount of water for full mixing, and performing vacuum drying at a temperature of 90 DEG C under an absolute pressure of 4.0 kPa till the water content is less than 2% to obtain the effective components of isatis roots. The method is mild in processing condition. The extraction ratio of a product is improved, and the obtained product has no residual solvent. The extraction time is shortened, and the extraction cost is lowered, so that the method is suitable for industrial production.
Description
Technical field
The invention belongs to aqueous enzymatic extraction technical field, the method being specifically related to aqueous enzymatic extraction effective ingredient of isatis root.
Background technology
Radix Isatidis is the dry root of cruciate flower plant Isatis indigotica Fort., is the main source of Radix Isatidis.Clinical conventional Chinese medicine, begins to be loaded in Shennong's Herbal, and bitter in the mouth is cold in nature, has heat-clearing and toxic substances removing, removing heat from blood sore-throat relieving function.For diseases such as maculae caused by violent heat pathogen, crimson tongue purple dark, mumps, scarlet fever, major part pestilence, erysipelas, carbuncle.
At present, the extracting method of endophytic effective ingredient has milling process, raw mill method, solvent extraction method, microwave extraction method, supercritical extraction.But the following defect existed: being that extraction ratio is low on the one hand, be that extraction time is long on the other hand, extraction cost is higher.Technological progress is endless, finds better extracting method by studying, makes extraction ratio higher, and extraction time is shorter, or the less extracting method of extraction cost, is highly significant.
Summary of the invention
Disengage to make effective ingredient of isatis root be prone to, thus obtaining the purer effective ingredient of isatis root compared with high yield pulp1, overcoming the extraction ratio of effective ingredient in traditional method for extracting Radix Isatidis, shortening extraction time, reduce cost, it is easy to realize factorial praluction.
The technical scheme that the present invention takes is:
The method of aqueous enzymatic extraction effective ingredient of isatis root, it is characterised in that specifically include following steps:
(1) taking Radix Isatidis pulverizing is 100 order fine powders, adds water and stirs, and heats to 50-55 DEG C of maintenance 8-12 minute, obtains seed slurry standby；
(2) adjust the acidity that seed is starched to be PH=5-6 with citric acid, add protease-fiber composite enzyme and carry out enzymolysis 45-55min, obtain enzymolysis solution standby；
(3) adding suitable quantity of water in above-mentioned enzymolysis solution, heat to 85-55 DEG C of lixiviate, sedimentation 3 separation, liquid, slag mixture centrifuge separates, and slag, aqueous phase are discharged, and use pressure filter filter pressing, obtain filtrate and filtering residue, and filtering residue is discharged, and filtrate is standby；
(4) filtrate adds after the phosphoric acid solution of 85% is sufficiently mixed, add after suitable quantity of water is sufficiently mixed, carry out vacuum drying when temperature, the absolute pressure 4.0kPa of 90 DEG C to water content less than 2%, obtain effective ingredient of isatis root.
The invention have the benefit that
(1) with the effective ingredient of aqueous enzymatic extraction Radix Isatidis, utilizing Radix Isatidis filtrate and the difference of filtering residue to be separated by effective ingredient, extraction ratio is up to more than 20%, and its extraction ratio is significantly larger than general solvent extraction method.
(2) aqueous enzymatic method treatment conditions of the present invention are gentle, can not only improve the extraction ratio of product, the product no solvent residue obtained, and extraction time shortens, and extraction cost is lower, is suitable for industrialized production.
Detailed description of the invention
The method of aqueous enzymatic extraction effective ingredient of isatis root, it is characterised in that specifically include following steps:
(1) taking Radix Isatidis pulverizing is 100 order fine powders, adds water and stirs, and heats to 50-55 DEG C of maintenance 8-12 minute, obtains seed slurry standby；
(2) adjust the acidity that seed is starched to be PH=5-6 with citric acid, add protease-fiber composite enzyme and carry out enzymolysis 45-55min, obtain enzymolysis solution standby；
(3) adding suitable quantity of water in above-mentioned enzymolysis solution, heat to 85-55 DEG C of lixiviate, sedimentation 3 separation, liquid, slag mixture centrifuge separates, and slag, aqueous phase are discharged, and use pressure filter filter pressing, obtain filtrate and filtering residue, and filtering residue is discharged, and filtrate is standby；
(4) filtrate adds after the phosphoric acid solution of 85% is sufficiently mixed, add after suitable quantity of water is sufficiently mixed, carry out vacuum drying when temperature, the absolute pressure 4.0kPa of 90 DEG C to water content less than 2%, obtain effective ingredient of isatis root.
Claims (1)
1. the method for aqueous enzymatic extraction effective ingredient of isatis root, it is characterised in that specifically include following steps:
(1) taking Radix Isatidis pulverizing is 100 order fine powders, adds water and stirs, and heats to 50-55 DEG C of maintenance 8-12 minute, obtains seed slurry standby；
(2) adjust the acidity that seed is starched to be PH=5-6 with citric acid, add protease-fiber composite enzyme and carry out enzymolysis 45-55min, obtain enzymolysis solution standby；
(3) adding suitable quantity of water in above-mentioned enzymolysis solution, heat to 85-55 DEG C of lixiviate, sedimentation 3 separation, liquid, slag mixture centrifuge separates, and slag, aqueous phase are discharged, and use pressure filter filter pressing, obtain filtrate and filtering residue, and filtering residue is discharged, and filtrate is standby；
(4) filtrate adds after the phosphoric acid solution of 85% is sufficiently mixed, add after suitable quantity of water is sufficiently mixed, carry out vacuum drying when temperature, the absolute pressure 4.0kPa of 90 DEG C to water content less than 2%, obtain effective ingredient of isatis root.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410755966.6A CN105726591A (en) | 2014-12-11 | 2014-12-11 | Method for extracting effective components of isatis roots through aqueous enzymatic method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410755966.6A CN105726591A (en) | 2014-12-11 | 2014-12-11 | Method for extracting effective components of isatis roots through aqueous enzymatic method |
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| CN105726591A true CN105726591A (en) | 2016-07-06 |
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| CN201410755966.6A Pending CN105726591A (en) | 2014-12-11 | 2014-12-11 | Method for extracting effective components of isatis roots through aqueous enzymatic method |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106397533A (en) * | 2016-12-13 | 2017-02-15 | 林蓬浩 | High-purity preparation process of isatis root plant protein |
| CN106496314A (en) * | 2016-12-13 | 2017-03-15 | 林蓬浩 | The preparation technology of antiviral Radix Isatidis vegetable protein |
| CN106518961A (en) * | 2016-12-13 | 2017-03-22 | 林蓬浩 | Extraction method of radix isatidis vegetable protein |
| CN106749508A (en) * | 2016-12-13 | 2017-05-31 | 林蓬浩 | The extracting method of high-purity Radix Isatidis vegetable protein |
| CN106749509A (en) * | 2016-12-13 | 2017-05-31 | 林蓬浩 | Improve the extracting method of Radix Isatidis vegetable protein purity |
| CN107349247A (en) * | 2017-05-23 | 2017-11-17 | 回音必集团安徽制药有限公司 | A kind of Radix Isatidis Optimized Extraction Process |
-
2014
- 2014-12-11 CN CN201410755966.6A patent/CN105726591A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106397533A (en) * | 2016-12-13 | 2017-02-15 | 林蓬浩 | High-purity preparation process of isatis root plant protein |
| CN106496314A (en) * | 2016-12-13 | 2017-03-15 | 林蓬浩 | The preparation technology of antiviral Radix Isatidis vegetable protein |
| CN106518961A (en) * | 2016-12-13 | 2017-03-22 | 林蓬浩 | Extraction method of radix isatidis vegetable protein |
| CN106749508A (en) * | 2016-12-13 | 2017-05-31 | 林蓬浩 | The extracting method of high-purity Radix Isatidis vegetable protein |
| CN106749509A (en) * | 2016-12-13 | 2017-05-31 | 林蓬浩 | Improve the extracting method of Radix Isatidis vegetable protein purity |
| CN107349247A (en) * | 2017-05-23 | 2017-11-17 | 回音必集团安徽制药有限公司 | A kind of Radix Isatidis Optimized Extraction Process |
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Application publication date: 20160706 |
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| WD01 | Invention patent application deemed withdrawn after publication |