CN106518961A - Extraction method of radix isatidis vegetable protein - Google Patents
Extraction method of radix isatidis vegetable protein Download PDFInfo
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- CN106518961A CN106518961A CN201611143032.2A CN201611143032A CN106518961A CN 106518961 A CN106518961 A CN 106518961A CN 201611143032 A CN201611143032 A CN 201611143032A CN 106518961 A CN106518961 A CN 106518961A
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- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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Abstract
The invention discloses an extraction method of radix isatidis vegetable protein. The method comprises the steps that 1, radix isatidis herbs are crushed; 2, water is added into radix isatidis particles, addition of the water is 10-15 times that of the radix isatidis particles, soaking is conducted for 5-6 h at the temperature ranging from 50 DEG C to 60 DEG C, centrifugal treatment is conducted for 3-5 min at 3,000-4,000 rpm, then, water is supplemented, the quantity of the supplemented water is 2-3 times that of the radix isatidis particles, and the mixture is subjected to centrifugal treatment for 20-25 min at 4,500-5,500 rpm; 3, supernatant is taken and subjected to ultrafiltration, filtrate is taken, freeze drying is conducted, and crude protein is obtained; 4, the crude protein is dissolved with water, the pH value is adjusted to be 4-6, and a crude protein solution is obtained; 5, cation exchange resin is used for treating the crude protein solution, and refined radix isatidis vegetable protein is obtained. According to the extraction method, the extraction efficiency of the vegetable protein is improved, and the applicability of the vegetable protein in the antiviral aspect can be expanded conveniently.
Description
Technical field
The present invention relates to a kind of extracting method of Radix Isatidis vegetable protein.
Background technology
In recent years, SARS, bird flu and influenza A H1N1 influenza virus are frequently broken out in China or even world wide, right
The life security of people and social economy cause greatly infringement.
For a long time, people and achieve good result by vaccine come pre- anti-virus, but vaccine is only and virus subtype
Just can be effective during matching.Virus variation is fast and breaks out with unpredictability, and vaccine research and production relatively lag behind.
For this drawback of vaccine, people are started widely studied antiviral drugs, are come to antiviral with this.Western medicine is disease-resistant
Cytotoxic drug has the features such as instant effect, curative effect are high, effect is accurate, but is respectively provided with specific aim per class medicine, specific just for certain
Virus work, because of the easy variability factor of virus antigen, cause some to make the application of the chemicalses of antiviral drugs
There is certain limitation.The effects such as many Chinese medicines have suppressing virus replication, prevent pathological changes caused by virus, Chinese medicine are exempted from by adjusting
Epidemic disease function, improve the comprehensive functions such as pulmonary circulation, antalgic and inflammation relieving, and then suppress or kill virus, with good preventing and treating virus
Effect.
It is also one of focus of research in recent years from Chinese medicine fully to excavate and find antiviral active substance.Radix Isatidis
With reliable antivirus action, it is the standing medicine of family.Radix Isatidis are cruciferae isatis (Isatis
Indigotica dry root), the effect of with heat-clearing and toxic substances removing, removing heat from blood sore-throat relieving, is usually used in treating influenza, parotitiss, epidemic febrile disease and sends out
The diseases of viral infection such as heat, epidemic encephalitis type B, hepatitis.Radix Isatidis complicated component, its material base and mechanism of action
Indefinite, production technology is still relatively backward.Accordingly, it would be desirable to the vegetable protein with antiviral effectiveness in Radix Isatidis is carried
Taking technique is further studied, to expand the application to the vegetable protein in Radix Isatidis.
The content of the invention
For above-mentioned technical problem, the present invention has designed and developed a kind of carrying for the higher Radix Isatidis vegetable protein of extraction efficiency
Take method.
The present invention provide technical scheme be：
A kind of extracting method of Radix Isatidis vegetable protein, including：
Step one, chromatogram of Radix Isatidis is ground into the Radix Isatidis granule that particle diameter is 300～350 μm；
Step 2, water is added in Radix Isatidis granule, 10～15 times for Radix Isatidis granule of the addition of water, 50～60
Soak 5～6 hours at DEG C, centrifugal treating 3～5 minutes under 3000～4000rpm, are then supplemented with water, supplementary water afterwards
2～3 times for Radix Isatidis granule are measured, centrifugal treating 20～25 minutes under 4500～5500rpm are placed in afterwards again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and lyophilization obtains crude protein；
Step 4, by crude protein water dissolution, adjust to pH value be 4～6, obtain crude protein solution；
Step 5, with cation exchange resin to processing crude protein solution, with water eluting repeatedly, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Preferably, in the extracting method of described Radix Isatidis vegetable protein, in the step one, by chromatogram of Radix Isatidis powder
It is broken into the Radix Isatidis granule that particle diameter is 310～320 μm.
Preferably, in the extracting method of described Radix Isatidis vegetable protein, in the step 2, in Radix Isatidis granule
Add water, 10 times for Radix Isatidis granule of the addition of water soaks 5～6 hours at 50～60 DEG C, afterwards 3000～
Centrifugal treating 3～5 minutes under 4000rpm, are then supplemented with water, and the supplementary water yield is 2 times of Radix Isatidis granule, is placed in again afterwards
Centrifugal treating 20～25 minutes under 4500～5500rpm.
Preferably, in the extracting method of described Radix Isatidis vegetable protein, in the step 2, in Radix Isatidis granule
Add water, 10 times for Radix Isatidis granule of the addition of water soaks 5 hours at 50 DEG C, afterwards under 3000～4000rpm from
The heart is processed 3～5 minutes, is then supplemented with water, 2 times for Radix Isatidis granule of the supplementary water yield, it is placed in 4500 afterwards again～
Centrifugal treating 20～25 minutes under 5500rpm.
Preferably, in the extracting method of described Radix Isatidis vegetable protein, in the step 2, in Radix Isatidis granule
Add water, 10 times for Radix Isatidis granule of the addition of water soaks 5 hours at 50 DEG C, afterwards centrifugal treating at 3,000 rpm
3 minutes, water is then supplemented with, the supplementary water yield is 2 times of Radix Isatidis granule, is placed in centrifugal treating 20 under 4500rpm afterwards again
Minute.
Preferably, in the extracting method of described Radix Isatidis vegetable protein, in the step 5, use cation exchange tree
Fat then carries out eluting to cation exchange resin with the ammonia that pH value is 8～9 to processing crude protein solution with water eluting repeatedly,
The eluent of water elution and ammonia eluting all is retained, lyophilization is carried out, refined Radix Isatidis vegetable protein is obtained.
The extracting method of Radix Isatidis vegetable protein of the present invention is by careful design extraction process, the plant extracted
Albumen purification mass percent more than 94%, improve the extraction efficiency of vegetable protein, contribute to expanding which anti-
The application of viral aspect.
Specific embodiment
The present invention is described in further detail below, with make those skilled in the art with reference to specification word being capable of evidence
To implement.
The present invention provides a kind of extracting method of Radix Isatidis vegetable protein, including：
Step one, chromatogram of Radix Isatidis is ground into the Radix Isatidis granule that particle diameter is 300～350 μm；
Step 2, water is added in Radix Isatidis granule, 10～15 times for Radix Isatidis granule of the addition of water, 50～60
Soak 5～6 hours at DEG C, centrifugal treating 3～5 minutes under 3000～4000rpm, are then supplemented with water, supplementary water afterwards
2～3 times for Radix Isatidis granule are measured, centrifugal treating 20～25 minutes under 4500～5500rpm are placed in afterwards again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and lyophilization obtains crude protein；
Step 4, by crude protein water dissolution, adjust to pH value be 4～6, obtain crude protein solution；
Step 5, with cation exchange resin to processing crude protein solution, with water eluting repeatedly, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
The extracting method of Radix Isatidis vegetable protein of the present invention is by careful design extraction process, the plant extracted
Albumen purification mass percent more than 94%, improve the extraction efficiency of vegetable protein, contribute to expanding which anti-
The application of viral aspect.
Preferably, in the extracting method of described Radix Isatidis vegetable protein, in the step one, by chromatogram of Radix Isatidis powder
It is broken into the Radix Isatidis granule that particle diameter is 310～320 μm.
Preferably, in the extracting method of described Radix Isatidis vegetable protein, in the step 2, in Radix Isatidis granule
Add water, 10 times for Radix Isatidis granule of the addition of water soaks 5～6 hours at 50～60 DEG C, afterwards 3000～
Centrifugal treating 3～5 minutes under 4000rpm, are then supplemented with water, and the supplementary water yield is 2 times of Radix Isatidis granule, is placed in again afterwards
Centrifugal treating 20～25 minutes under 4500～5500rpm.
Preferably, in the extracting method of described Radix Isatidis vegetable protein, in the step 2, in Radix Isatidis granule
Add water, 10 times for Radix Isatidis granule of the addition of water soaks 5 hours at 50 DEG C, afterwards under 3000～4000rpm from
The heart is processed 3～5 minutes, is then supplemented with water, 2 times for Radix Isatidis granule of the supplementary water yield, it is placed in 4500 afterwards again～
Centrifugal treating 20～25 minutes under 5500rpm.
Preferably, in the extracting method of described Radix Isatidis vegetable protein, in the step 2, in Radix Isatidis granule
Add water, 10 times for Radix Isatidis granule of the addition of water soaks 5 hours at 50 DEG C, afterwards centrifugal treating at 3,000 rpm
3 minutes, water is then supplemented with, the supplementary water yield is 2 times of Radix Isatidis granule, is placed in centrifugal treating 20 under 4500rpm afterwards again
Minute.
Preferably, in the extracting method of described Radix Isatidis vegetable protein, in the step 5, use cation exchange tree
Fat then carries out eluting to cation exchange resin with the ammonia that pH value is 8～9 to processing crude protein solution with water eluting repeatedly,
The eluent of water elution and ammonia eluting all is retained, lyophilization is carried out, refined Radix Isatidis vegetable protein is obtained.
Embodiment one
Step one, chromatogram of Radix Isatidis is ground into the Radix Isatidis granule that particle diameter is 300～310 μm；
Step 2, water is added in Radix Isatidis granule, the addition of water is 10 times of Radix Isatidis granule, is soaked at 50 DEG C
5 hours, then centrifugal treating 3 minutes at 3,000 rpm afterwards were supplemented with water, 2 times for Radix Isatidis granule of the supplementary water yield,
It is placed in centrifugal treating 20 minutes under 4500rpm afterwards again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and lyophilization obtains crude protein；
Step 4, by crude protein water dissolution, adjust to pH value be 4, obtain crude protein solution；
Step 5, with cation exchange resin to processing crude protein solution, with water eluting repeatedly, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94%.
Embodiment two
Step one, chromatogram of Radix Isatidis is ground into the Radix Isatidis granule that particle diameter is 300～320 μm；
Step 2, water is added in Radix Isatidis granule, the addition of water is 15 times of Radix Isatidis granule, is soaked at 60 DEG C
6 hours, centrifugal treating 5 minutes under 4000rpm, were then supplemented with water afterwards, and the supplementary water yield is 3 times of Radix Isatidis granule,
It is placed in centrifugal treating 25 minutes under 5500rpm afterwards again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and lyophilization obtains crude protein；
Step 4, by crude protein water dissolution, adjust to pH value be 6, obtain crude protein solution；
Step 5, with cation exchange resin to processing crude protein solution, with water eluting repeatedly, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94.2%.
Embodiment three
Step one, chromatogram of Radix Isatidis is ground into the Radix Isatidis granule that particle diameter is 340～350 μm；
Step 2, water is added in Radix Isatidis granule, the addition of water is 15 times of Radix Isatidis granule, is soaked at 60 DEG C
6 hours, centrifugal treating 5 minutes under 4000rpm, were then supplemented with water afterwards, and the supplementary water yield is 3 times of Radix Isatidis granule,
It is placed in centrifugal treating 25 minutes under 5500rpm afterwards again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and lyophilization obtains crude protein；
Step 4, by crude protein water dissolution, adjust to pH value be 6, obtain crude protein solution；
Step 5, with cation exchange resin to processing crude protein solution, with water eluting repeatedly, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94.5%.
Example IV
Step one, chromatogram of Radix Isatidis is ground into the Radix Isatidis granule that particle diameter is 310～320 μm；
Step 2, water is added in Radix Isatidis granule, the addition of water is 10 times of Radix Isatidis granule, is soaked at 50 DEG C
5 hours, then centrifugal treating 3 minutes at 3,000 rpm afterwards were supplemented with water, 2 times for Radix Isatidis granule of the supplementary water yield,
It is placed in centrifugal treating 20 minutes under 4500rpm afterwards again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and lyophilization obtains crude protein；
Step 4, by crude protein water dissolution, adjust to pH value be 6, obtain crude protein solution；
Step 5, with cation exchange resin to processing crude protein solution, with water eluting repeatedly, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94.3%.
Embodiment five
Step one, chromatogram of Radix Isatidis is ground into the Radix Isatidis granule that particle diameter is 310～325 μm；
Step 2, water is added in Radix Isatidis granule, the addition of water is 10 times of Radix Isatidis granule, is soaked at 50 DEG C
5.5 hours, centrifugal treating 3 minutes at 3,000 rpm, were then supplemented with water afterwards, and the supplementary water yield is the 2 of Radix Isatidis granule
Times, it is placed in centrifugal treating 20 minutes under 4500rpm afterwards again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and lyophilization obtains crude protein；
Step 4, by crude protein water dissolution, adjust to pH value be 6, obtain crude protein solution；
Step 5, with cation exchange resin to processing crude protein solution, with water eluting repeatedly, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94.3%.
Embodiment six
Step one, chromatogram of Radix Isatidis is ground into the Radix Isatidis granule that particle diameter is 310～320 μm；
Step 2, water is added in Radix Isatidis granule, the addition of water is 10 times of Radix Isatidis granule, is soaked at 50 DEG C
5 hours, centrifugal treating 3 minutes under 3500rpm, were then supplemented with water afterwards, and the supplementary water yield is 2 times of Radix Isatidis granule,
It is placed in centrifugal treating 22 minutes under 4500rpm afterwards again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and lyophilization obtains crude protein；
Step 4, by crude protein water dissolution, adjust to pH value be 6, obtain crude protein solution；
Step 5, with cation exchange resin to processing crude protein solution, with water eluting repeatedly, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94.2%.
Embodiment seven
Step one, chromatogram of Radix Isatidis is ground into the Radix Isatidis granule that particle diameter is 310～320 μm；
Step 2, water is added in Radix Isatidis granule, the addition of water is 10 times of Radix Isatidis granule, is soaked at 55 DEG C
5 hours, then centrifugal treating 3 minutes at 3,000 rpm afterwards were supplemented with water, 3 times for Radix Isatidis granule of the supplementary water yield,
It is placed in centrifugal treating 20 minutes under 4500rpm afterwards again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and lyophilization obtains crude protein；
Step 4, by crude protein water dissolution, adjust to pH value be 6, obtain crude protein solution；
Step 5, with cation exchange resin to processing crude protein solution, with water eluting repeatedly, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94.3%.
Embodiment eight
Step one, chromatogram of Radix Isatidis is ground into the Radix Isatidis granule that particle diameter is 310～320 μm；
Step 2, water is added in Radix Isatidis granule, the addition of water is 10 times of Radix Isatidis granule, is soaked at 50 DEG C
5 hours, then centrifugal treating 3 minutes at 3,000 rpm afterwards were supplemented with water, 2 times for Radix Isatidis granule of the supplementary water yield,
It is placed in centrifugal treating 24 minutes under 4550rpm afterwards again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and lyophilization obtains crude protein；
Step 4, by crude protein water dissolution, adjust to pH value be 6, obtain crude protein solution；
Step 5, with cation exchange resin to processing crude protein solution, with water eluting repeatedly, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94.2%.
Embodiment nine
Step one, chromatogram of Radix Isatidis is ground into the Radix Isatidis granule that particle diameter is 310～320 μm；
Step 2, water is added in Radix Isatidis granule, the addition of water is 10 times of Radix Isatidis granule, is soaked at 55 DEG C
6 hours, then centrifugal treating 3 minutes at 3,000 rpm afterwards were supplemented with water, 3 times for Radix Isatidis granule of the supplementary water yield,
It is placed in centrifugal treating 20 minutes under 4500rpm afterwards again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and lyophilization obtains crude protein；
Step 4, by crude protein water dissolution, adjust to pH value be 6, obtain crude protein solution；
Step 5, with cation exchange resin to processing crude protein solution, with water eluting repeatedly, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94.1%.
Embodiment ten
Step one, chromatogram of Radix Isatidis is ground into the Radix Isatidis granule that particle diameter is 320～330 μm；
Step 2, water is added in Radix Isatidis granule, the addition of water is 10 times of Radix Isatidis granule, is soaked at 50 DEG C
5 hours, then centrifugal treating 3 minutes at 3,000 rpm afterwards were supplemented with water, 2 times for Radix Isatidis granule of the supplementary water yield,
It is placed in centrifugal treating 20 minutes under 4500rpm afterwards again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and lyophilization obtains crude protein；
Step 4, by crude protein water dissolution, adjust to pH value be 6, obtain crude protein solution；
Step 5, with cation exchange resin to processing crude protein solution, with water eluting repeatedly, leave and take eluent freezing dry
It is dry, obtain refined Radix Isatidis vegetable protein.
Finally, the shared mass percent in purification of vegetable protein is 94.5%.
Comparative example
Radix Isatidis vegetable protein is extracted using existing process, mass percent of the vegetable protein in purification is only
Account for 87%.
Although embodiment of the present invention is disclosed as above, which is not restricted to listed by description and embodiment
With, it can be applied to various suitable the field of the invention completely, for those skilled in the art, can be easily
Other modification is realized, therefore under the general concept limited without departing substantially from claim and equivalency range, the present invention is not limited
In specific details.
Claims (6)
1. a kind of extracting method of Radix Isatidis vegetable protein, it is characterised in that include：
Step one, chromatogram of Radix Isatidis is ground into the Radix Isatidis granule that particle diameter is 300～350 μm；
Step 2, water is added in Radix Isatidis granule, 10～15 times for Radix Isatidis granule of the addition of water, at 50～60 DEG C
Immersion 5～6 hours, centrifugal treating 3～5 minutes under 3000～4000rpm, are then supplemented with water afterwards, and the supplementary water yield is
2～3 times of Radix Isatidis granule, are placed in centrifugal treating 20～25 minutes under 4500～5500rpm afterwards again；
Step 3, supernatant is taken, ultrafiltration takes filtrate, and lyophilization obtains crude protein；
Step 4, by crude protein water dissolution, adjust to pH value be 4～6, obtain crude protein solution；
Step 5, with cation exchange resin to processing crude protein solution, with water eluting repeatedly, leave and take eluent lyophilization,
Obtain refined Radix Isatidis vegetable protein.
2. the extracting method of Radix Isatidis vegetable protein as claimed in claim 1, it is characterised in that in the step one, by plate
Blue root herb is ground into the Radix Isatidis granule that particle diameter is 310～320 μm.
3. the extracting method of Radix Isatidis vegetable protein as claimed in claim 1, it is characterised in that in the step 2, to plate
Water is added in blue root granule, the addition of water is 10 times of Radix Isatidis granule, is soaked 5～6 hours, Zhi Hou at 50～60 DEG C
Centrifugal treating 3～5 minutes under 3000～4000rpm, are then supplemented with water, and the supplementary water yield is 2 times of Radix Isatidis granule, afterwards
Centrifugal treating 20～25 minutes under 4500～5500rpm are placed in again.
4. the extracting method of Radix Isatidis vegetable protein as claimed in claim 3, it is characterised in that in the step 2, to plate
Add water in blue root granule, 10 times for Radix Isatidis granule of the addition of water soaks 5 hours at 50 DEG C, afterwards 3000～
Centrifugal treating 3～5 minutes under 4000rpm, are then supplemented with water, and the supplementary water yield is 2 times of Radix Isatidis granule, is placed in again afterwards
Centrifugal treating 20～25 minutes under 4500～5500rpm.
5. the extracting method of Radix Isatidis vegetable protein as claimed in claim 4, it is characterised in that in the step 2, to plate
Water is added in blue root granule, the addition of water is 10 times of Radix Isatidis granule, is soaked 5 hours, afterwards in 3000rpm at 50 DEG C
Lower centrifugal treating 3 minutes, is then supplemented with water, and the supplementary water yield is 2 times of Radix Isatidis granule, is placed under 4500rpm again afterwards
Centrifugal treating 20 minutes.
6. the extracting method of Radix Isatidis vegetable protein as claimed in claim 1, it is characterised in that in the step 5, with sun
Ion exchange resin to processing crude protein solution, with water eluting repeatedly, then with the ammonia that pH value is 8～9 to cation exchange tree
Fat carries out eluting, and the eluent of water elution and ammonia eluting all is retained, and carries out lyophilization, obtains refined Baphicacanthus cusia
Root vegetable protein.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2990132A1 (en) * | 2012-05-04 | 2013-11-08 | Cocagne & Cie | Proteinic extract obtained from Isatis tinctoria, useful as anti-aging agent in a cosmetic, dermatological and pharmaceutical composition for treating/preventing skin alterations, preferably dermis alterations, comprises proteins |
| CN103641899A (en) * | 2013-11-01 | 2014-03-19 | 深圳职业技术学院 | Isatis root vegetable proteins or polypeptides, extraction method thereof, and application thereof in antiviral drug |
| CN103694363A (en) * | 2013-11-27 | 2014-04-02 | 广东药学院 | Preparation method of refined polysaccharide of isatis root |
| CN105237615A (en) * | 2015-09-25 | 2016-01-13 | 苏州蔻美新材料有限公司 | Method used for simultaneous extraction of radix isatidis polypeptides and polysaccharides |
| CN105726591A (en) * | 2014-12-11 | 2016-07-06 | 青岛清泉生物科技有限公司 | Method for extracting effective components of isatis roots through aqueous enzymatic method |
-
2016
- 2016-12-13 CN CN201611143032.2A patent/CN106518961A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2990132A1 (en) * | 2012-05-04 | 2013-11-08 | Cocagne & Cie | Proteinic extract obtained from Isatis tinctoria, useful as anti-aging agent in a cosmetic, dermatological and pharmaceutical composition for treating/preventing skin alterations, preferably dermis alterations, comprises proteins |
| CN103641899A (en) * | 2013-11-01 | 2014-03-19 | 深圳职业技术学院 | Isatis root vegetable proteins or polypeptides, extraction method thereof, and application thereof in antiviral drug |
| CN103694363A (en) * | 2013-11-27 | 2014-04-02 | 广东药学院 | Preparation method of refined polysaccharide of isatis root |
| CN105726591A (en) * | 2014-12-11 | 2016-07-06 | 青岛清泉生物科技有限公司 | Method for extracting effective components of isatis roots through aqueous enzymatic method |
| CN105237615A (en) * | 2015-09-25 | 2016-01-13 | 苏州蔻美新材料有限公司 | Method used for simultaneous extraction of radix isatidis polypeptides and polysaccharides |
Non-Patent Citations (3)
| Title |
|---|
| 于鑫鑫 等: "板蓝根有效成分提取的影响因素", 《养殖技术顾问》 * |
| 肖平: "基于清热解毒板蓝根蛋白及相关成分研究", 《中国学位论文全文数据库》 * |
| 胡兴昌 等: "板蓝根生药中蛋白质提取方法的比较", 《上海师范大学学报（自然科学版）》 * |
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