CN107349247A - A kind of Radix Isatidis Optimized Extraction Process - Google Patents
A kind of Radix Isatidis Optimized Extraction Process Download PDFInfo
- Publication number
- CN107349247A CN107349247A CN201710365797.9A CN201710365797A CN107349247A CN 107349247 A CN107349247 A CN 107349247A CN 201710365797 A CN201710365797 A CN 201710365797A CN 107349247 A CN107349247 A CN 107349247A
- Authority
- CN
- China
- Prior art keywords
- water
- radix isatidis
- minutes
- bacterium solution
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000010231 banlangen Substances 0.000 title claims abstract description 38
- 238000000605 extraction Methods 0.000 title claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 32
- 238000003809 water extraction Methods 0.000 claims abstract description 23
- 239000002994 raw material Substances 0.000 claims abstract description 14
- 238000001556 precipitation Methods 0.000 claims abstract description 11
- 238000011084 recovery Methods 0.000 claims abstract description 10
- 238000001694 spray drying Methods 0.000 claims abstract description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 27
- 239000000243 solution Substances 0.000 claims description 27
- 238000000855 fermentation Methods 0.000 claims description 23
- 230000004151 fermentation Effects 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 22
- 239000000706 filtrate Substances 0.000 claims description 20
- 239000000463 material Substances 0.000 claims description 18
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 16
- 239000000843 powder Substances 0.000 claims description 15
- 239000012535 impurity Substances 0.000 claims description 14
- 230000008014 freezing Effects 0.000 claims description 13
- 238000007710 freezing Methods 0.000 claims description 13
- 241000894006 Bacteria Species 0.000 claims description 10
- 150000001875 compounds Chemical class 0.000 claims description 10
- 239000012141 concentrate Substances 0.000 claims description 10
- 239000006228 supernatant Substances 0.000 claims description 10
- 238000005406 washing Methods 0.000 claims description 10
- 239000007864 aqueous solution Substances 0.000 claims description 8
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 8
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 8
- 239000003814 drug Substances 0.000 claims description 7
- 241000194108 Bacillus licheniformis Species 0.000 claims description 5
- 241000186000 Bifidobacterium Species 0.000 claims description 5
- 241000186660 Lactobacillus Species 0.000 claims description 5
- 125000004122 cyclic group Chemical group 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 239000000047 product Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- 239000011149 active material Substances 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 6
- 230000008901 benefit Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 239000002253 acid Substances 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 241000334160 Isatis Species 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000001986 anti-endotoxic effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000003359 percent control normalization Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of Radix Isatidis Optimized Extraction Process, following steps are specifically included：（1）Pretreatment of raw material；（2）Water extract-alcohol precipitation operates；（3）Spray drying, obtains finished product.It is more shorter than the water extraction time in the prior art using a kind of Radix Isatidis Optimized Extraction Process provided by the invention by experiment, but it is higher to the recovery rate of active material in Radix Isatidis, the pharmacy cost of enterprise can be substantially reduced, is worth of widely use.
Description
Technical field
A kind of purification technique field of bioactive substance of the present invention, and in particular to Radix Isatidis Optimized Extraction Process.
Background technology
Radix Isatidis is the dry root of cruciferae isatis, has extensive cultivation in China, is that China commonly uses for many years
Heat-clearing and detoxifying drug, have antiviral, antiendotoxin, antipyretic-antalgic, anti-inflammatory, activate blood circulation and disperse blood clots, anticancer the effect of.
The technique of the extensive extraction Radix Isatidis of industrialization, is often water extraction and alcohol precipitation method, because its have it is inexpensive, simple to operate etc.
Advantage, but this method extraction efficiency is low, expends time length, there is very big optimization space.
The content of the invention
In order to solve the above problems, the invention provides a kind of Radix Isatidis Optimized Extraction Process, traditional industry water is optimized
Alcohol deposition method is carried, makes Radix Isatidis.
The present invention is achieved through the following technical solutions：
A kind of Radix Isatidis Optimized Extraction Process, specifically includes following steps：
（1）Pretreatment of raw material
Raw material Radix Isatidis is put into medicine-washing machine, the sodium bicarbonate aqueous solution that use quality fraction is 2% embathes 5-8 minute heel row
Water, converting, clear water is secondary to embathe 1-2 minutes, filters out medicinal material, drains away the water, and is placed in medicine cutter and cuts into slices, is then fed into fermentation tank
In, the compound bacteria bacterium solution of 0.3-0.6 times of Radix Isatidis mass fraction is added, 90-120 minutes are sealed by fermentation at a temperature of 20-25 DEG C,
Filtered after washing, obtain fermentation medicinal material, then fermentation medicinal material is dried to water content 1% using 10-15 DEG C of cold wind cyclic drying system
Hereinafter, freezing processing 45-55 minutes in -15 DEG C of freezers are subsequently placed in, are taken out after the completion of freezing processing, are ground to 80 mesh,
Obtain medicinal powder；The compound bacteria bacterium solution, by lactobacillus bacterium solution, Bifidobacterium bacterium solution, Bacillus licheniformis liquid in mass ratio 5:3:
It is made after 8-12 mixing；
（2）Water extract-alcohol precipitation operates
By step（1）The obtained medicinal powder is added in extractor, adds the water of the 20-25 times of medicinal powder mass fraction, is carried out
Abundant water extraction, is first heated to 80-85 DEG C during water extraction, be incubated 5-10 minutes, then treats that it naturally cools to 45-50 DEG C, then
Secondary 80-85 DEG C of heating, so handles 1.5-2 hours, filters to obtain filter residue and first-time filtrate, the filter residue then is used into phase repeatedly
Carried with method secondary water, filtering and impurity removing obtains secondary filtrate, merges the first-time filtrate and secondary filtrate, is concentrated under reduced pressure into relatively close
Degree 1.24, obtains concentrate, concentrate is slowly added into dense ethanol solution, until concentration of alcohol reaches 62-65%, stands, centrifuging and taking
Supernatant, the supernatant recovery ethanol produce decoction；
（3）Spray drying, obtains finished product.
Beneficial effects of the present invention：Raw material Radix Isatidis is embathed by the sodium bicarbonate aqueous solution that mass fraction is 2%, energy
The acid impurities remained in medicinal material are effectively removed, while play the effect of sterilizing, it is effective by the Radix Isatidis of cold fermentation
Eliminate the impurity such as protein, lipid, facilitate subsequent purification to handle, at the same avoid fermentation temperature it is too high caused by medicine effectively into
Analyze, then dried using cold wind, cryogenic freezing carries out pulverization process again, further avoid the precipitation of effective ingredient,
The efficiency of follow-up water extraction operation is improved simultaneously；Using the method for alternating temperature water extraction, avoid effective ingredient and divide at high temperature
Solution, while the hydrolysis of water-solubility impurity is effectively reduced, the generation of impurity is avoided, improves the recovery rate of Radix Isatidis, simultaneously
The method of alternating temperature reduces heating cost, improves the economic benefit of enterprise；By experiment, a kind of plate provided by the invention is used
Blue root Optimized Extraction Process, it is more shorter than the water extraction time in the prior art, but it is higher to the recovery rate of active material in Radix Isatidis,
The pharmacy cost of enterprise can be substantially reduced, is worth of widely use.
Embodiment
Embodiment 1
A kind of Radix Isatidis Optimized Extraction Process, specifically includes following steps：
（1）Pretreatment of raw material
Raw material Radix Isatidis is put into medicine-washing machine, the sodium bicarbonate aqueous solution that use quality fraction is 2% embathes draining after 5 minutes,
Converting, clear water is secondary to be embathed 1 minute, is filtered out medicinal material, is drained away the water, and is placed in medicine cutter and is cut into slices, is then fed into fermentation tank, adds
Enter the compound bacteria bacterium solution of 0.3 times of Radix Isatidis mass fraction, be sealed by fermentation 90 minutes at a temperature of 20 DEG C, filter, must send out after washing
Ferment medicinal material, then fermentation medicinal material is dried to water content below 1% using 10 DEG C of cold wind cyclic drying systems, it is subsequently placed in -15 DEG C
Freezing processing 45 minutes, take out after the completion of freezing processing in freezer, grind to 80 mesh, obtain medicinal powder；The compound bacteria bacterium solution,
By lactobacillus bacterium solution, Bifidobacterium bacterium solution, Bacillus licheniformis liquid in mass ratio 5:3:It is made after 8 mixing；Raw material plate is blue
Root embathes by the sodium bicarbonate aqueous solution that mass fraction is 2%, can effectively remove the acid impurities remained in medicinal material, rise simultaneously
To the effect of sterilizing, the Radix Isatidis by cold fermentation effectively eliminates the impurity such as protein, lipid, facilitates subsequent purification
Processing, while avoid the too high caused effective ingredient of fermentation temperature from separating out, then dried using cold wind, cryogenic freezing is entered again
Row pulverization process, further avoids the precipitation of effective ingredient, while improves the efficiency of follow-up water extraction operation；
（2）Water extract-alcohol precipitation operates
By step（1）The obtained medicinal powder is added in extractor, adds the water of 20 times of medicinal powder mass fractions, is carried out abundant
Water extraction, 80 DEG C are first heated to during water extraction, are incubated 5 minutes, then treat that it naturally cools to 45 DEG C, heat 80 DEG C again, such as
This is handled 1.5 hours repeatedly, filters to obtain filter residue and first-time filtrate, and the filter residue then is used into the secondary water extraction of same procedure, mistake
Miscellaneous secondary filtrate is filtered out, merges the first-time filtrate and secondary filtrate, is concentrated under reduced pressure into relative density 1.24, obtains concentrate,
Concentrate is slowly added to dense ethanol solution, until concentration of alcohol reaches 62%, stood, centrifuging and taking supernatant, the supernatant returns
Receive ethanol and produce decoction；Using the method for alternating temperature water extraction, avoid effective ingredient and decompose at high temperature, while effectively reduce
The hydrolysis of water-solubility impurity, avoids the generation of impurity, improves the recovery rate of Radix Isatidis, while the method for alternating temperature reduces
Heating cost, improve the economic benefit of enterprise；
（3）Spray drying, obtains finished product.
Embodiment 2
A kind of Radix Isatidis Optimized Extraction Process, specifically includes following steps：
（1）Pretreatment of raw material
Raw material Radix Isatidis is put into medicine-washing machine, the sodium bicarbonate aqueous solution that use quality fraction is 2% embathes draining after 8 minutes,
Converting, clear water is secondary to be embathed 2 minutes, is filtered out medicinal material, is drained away the water, and is placed in medicine cutter and is cut into slices, is then fed into fermentation tank, adds
Enter the compound bacteria bacterium solution of 0.6 times of Radix Isatidis mass fraction, be sealed by fermentation 120 minutes at a temperature of 25 DEG C, filter, must send out after washing
Ferment medicinal material, then fermentation medicinal material is dried to water content below 1% using 15 DEG C of cold wind cyclic drying systems, it is subsequently placed in -15 DEG C
Freezing processing 55 minutes, take out after the completion of freezing processing in freezer, grind to 80 mesh, obtain medicinal powder；The compound bacteria bacterium solution,
By lactobacillus bacterium solution, Bifidobacterium bacterium solution, Bacillus licheniformis liquid in mass ratio 5:3:It is made after 12 mixing；Raw material plate is blue
Root embathes by the sodium bicarbonate aqueous solution that mass fraction is 2%, can effectively remove the acid impurities remained in medicinal material, rise simultaneously
To the effect of sterilizing, the Radix Isatidis by cold fermentation effectively eliminates the impurity such as protein, lipid, facilitates subsequent purification
Processing, while avoid the too high caused effective ingredient of fermentation temperature from separating out, then dried using cold wind, cryogenic freezing is entered again
Row pulverization process, further avoids the precipitation of effective ingredient, while improves the efficiency of follow-up water extraction operation；
（2）Water extract-alcohol precipitation operates
By step（1）The obtained medicinal powder is added in extractor, adds the water of 25 times of medicinal powder mass fractions, is carried out abundant
Water extraction, 85 DEG C are first heated to during water extraction, are incubated 10 minutes, then treat that it naturally cools to 50 DEG C, heat 85 DEG C again, such as
This is handled 2 hours repeatedly, filters to obtain filter residue and first-time filtrate, and the filter residue then is used into the secondary water extraction of same procedure, filtering
Clean to obtain secondary filtrate, merges the first-time filtrate and secondary filtrate, is concentrated under reduced pressure into relative density 1.24, obtains concentrate, will
Concentrate is slowly added to dense ethanol solution, until concentration of alcohol reaches 65%, stands, centrifuging and taking supernatant, the supernatant recovery
Ethanol produces decoction；Using the method for alternating temperature water extraction, avoid effective ingredient and decompose at high temperature, effectively reduce simultaneously
The hydrolysis of water-solubility impurity, the generation of impurity is avoided, improve the recovery rate of Radix Isatidis, while the method for alternating temperature is reduced and added
Hot cost, improve the economic benefit of enterprise；
（3）Spray drying, obtains finished product.
Comparative example 1
This comparative example 1 compared with Example 1, without step（1）The operation, using existing Radix Isatidis pretreatment side
Method, method and step all same in addition.
Comparative example 2
This comparative example 2 compared with Example 2, without step（2）The operation, operated using only conventional water extract-alcohol precipitation,
Method and step all same in addition.
Control group
Existing industrial Extraction Technology of Radix Isatidis.
The extraction of Radix Isatidis is carried out using above-mentioned 5 kinds of methods respectively, in the extraction time and extract that determine every kind of method
The content of active component, as a result such as table 1：
Table 1
| Extraction time（min） | Active component content（%） | |
| Embodiment 1 | 90 | 0.5% |
| Embodiment 2 | 120 | 0.6% |
| Comparative example 1 | 90 | 0.2% |
| Comparative example 2 | 180 | 0.4% |
| Control group | 180 | 0.5% |
As shown in Table 1, using a kind of Radix Isatidis Optimized Extraction Process provided by the invention, than the water extraction time in the prior art more
It is short, but it is higher to the recovery rate of active material in Radix Isatidis, the pharmacy cost of enterprise can be substantially reduced, being worthy to be popularized makes
With.
Claims (2)
1. a kind of Radix Isatidis Optimized Extraction Process, it is characterised in that specifically include following steps：
（1）Pretreatment of raw material
Raw material Radix Isatidis is put into medicine-washing machine, the sodium bicarbonate aqueous solution that use quality fraction is 2% embathes 5-8 minute heel row
Water, converting, clear water is secondary to embathe 1-2 minutes, filters out medicinal material, drains away the water, and is placed in medicine cutter and cuts into slices, is then fed into fermentation tank
In, the compound bacteria bacterium solution of 0.3-0.6 times of Radix Isatidis mass fraction is added, 90-120 minutes are sealed by fermentation at a temperature of 20-25 DEG C,
Filtered after washing, obtain fermentation medicinal material, then fermentation medicinal material is dried to water content 1% using 10-15 DEG C of cold wind cyclic drying system
Hereinafter, freezing processing 45-55 minutes in -15 DEG C of freezers are subsequently placed in, are taken out after the completion of freezing processing, are ground to 80 mesh,
Obtain medicinal powder；The compound bacteria bacterium solution, by lactobacillus bacterium solution, Bifidobacterium bacterium solution, Bacillus licheniformis liquid in mass ratio 5:3:
It is made after 8-12 mixing；
（2）Water extract-alcohol precipitation operates
By step（1）The obtained medicinal powder is added in extractor, adds the water of the 20-25 times of medicinal powder mass fraction, is carried out
Abundant water extraction, is first heated to 80-85 DEG C during water extraction, be incubated 5-10 minutes, then treats that it naturally cools to 45-50 DEG C, then
Secondary 80-85 DEG C of heating, so handles 1.5-2 hours, filters to obtain filter residue and first-time filtrate, the filter residue then is used into phase repeatedly
Carried with method secondary water, filtering and impurity removing obtains secondary filtrate, merges the first-time filtrate and secondary filtrate, is concentrated under reduced pressure into relatively close
Degree 1.24, obtains concentrate, concentrate is slowly added into dense ethanol solution, until concentration of alcohol reaches 62-65%, stands, centrifuging and taking
Supernatant, the supernatant recovery ethanol produce decoction；
（3）Spray drying, obtains finished product.
2. a kind of Radix Isatidis Optimized Extraction Process according to claim 1, it is characterised in that specifically include following steps：
（1）Pretreatment of raw material
Raw material Radix Isatidis is put into medicine-washing machine, the sodium bicarbonate aqueous solution that use quality fraction is 2% embathes draining after 8 minutes,
Converting, clear water is secondary to be embathed 2 minutes, is filtered out medicinal material, is drained away the water, and is placed in medicine cutter and is cut into slices, is then fed into fermentation tank, adds
Enter the compound bacteria bacterium solution of 0.6 times of Radix Isatidis mass fraction, be sealed by fermentation 120 minutes at a temperature of 25 DEG C, filter, must send out after washing
Ferment medicinal material, then fermentation medicinal material is dried to water content below 1% using 15 DEG C of cold wind cyclic drying systems, it is subsequently placed in -15 DEG C
Freezing processing 55 minutes, take out after the completion of freezing processing in freezer, grind to 80 mesh, obtain medicinal powder；The compound bacteria bacterium solution,
By lactobacillus bacterium solution, Bifidobacterium bacterium solution, Bacillus licheniformis liquid in mass ratio 5:3:It is made after 12 mixing；
（2）Water extract-alcohol precipitation operates
By step（1）The obtained medicinal powder is added in extractor, adds the water of 25 times of medicinal powder mass fractions, is carried out abundant
Water extraction, 85 DEG C are first heated to during water extraction, are incubated 10 minutes, then treat that it naturally cools to 50 DEG C, heat 85 DEG C again, such as
This is handled 2 hours repeatedly, filters to obtain filter residue and first-time filtrate, and the filter residue then is used into the secondary water extraction of same procedure, filtering
Clean to obtain secondary filtrate, merges the first-time filtrate and secondary filtrate, is concentrated under reduced pressure into relative density 1.24, obtains concentrate, will
Concentrate is slowly added to dense ethanol solution, until concentration of alcohol reaches 65%, stands, centrifuging and taking supernatant, the supernatant recovery
Ethanol produces decoction；
（3）Spray drying, obtains finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710365797.9A CN107349247A (en) | 2017-05-23 | 2017-05-23 | A kind of Radix Isatidis Optimized Extraction Process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710365797.9A CN107349247A (en) | 2017-05-23 | 2017-05-23 | A kind of Radix Isatidis Optimized Extraction Process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107349247A true CN107349247A (en) | 2017-11-17 |
Family
ID=60271593
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710365797.9A Pending CN107349247A (en) | 2017-05-23 | 2017-05-23 | A kind of Radix Isatidis Optimized Extraction Process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107349247A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109010407A (en) * | 2018-09-11 | 2018-12-18 | 华润三九（雅安）药业有限公司 | A kind of Radix Isatidis syrup and preparation method thereof |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1157732A (en) * | 1996-02-22 | 1997-08-27 | 大连经济技术开发区蚂蚁保健制品开发公司 | Ant queen jelly oral liquid and its manufacturing method |
| CN1456265A (en) * | 2003-02-21 | 2003-11-19 | 毛友昌 | Isatis root effervescent particle preparing method |
| CN101036684A (en) * | 2006-03-15 | 2007-09-19 | 上海医药工业研究院 | Isatis-Root extractive and the purifying method and the medicine combination |
| CN101396382A (en) * | 2007-09-27 | 2009-04-01 | 上海市脑血管病防治研究所 | Composite isatis root orally disintegrating tablet and preparation method thereof |
| CN103134272A (en) * | 2011-11-23 | 2013-06-05 | 天津天士力现代中药资源有限公司 | Radix isatidis extraction drying method |
| CN104288194A (en) * | 2014-10-30 | 2015-01-21 | 黄琴 | Preparation method for compound radix isatidis granules |
| CN104814988A (en) * | 2015-05-20 | 2015-08-05 | 福州闽海药业有限公司 | Preparation method of indigowoad root granules |
| CN105237615A (en) * | 2015-09-25 | 2016-01-13 | 苏州蔻美新材料有限公司 | Method used for simultaneous extraction of radix isatidis polypeptides and polysaccharides |
| CN105726591A (en) * | 2014-12-11 | 2016-07-06 | 青岛清泉生物科技有限公司 | Method for extracting effective components of isatis roots through aqueous enzymatic method |
-
2017
- 2017-05-23 CN CN201710365797.9A patent/CN107349247A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1157732A (en) * | 1996-02-22 | 1997-08-27 | 大连经济技术开发区蚂蚁保健制品开发公司 | Ant queen jelly oral liquid and its manufacturing method |
| CN1456265A (en) * | 2003-02-21 | 2003-11-19 | 毛友昌 | Isatis root effervescent particle preparing method |
| CN101036684A (en) * | 2006-03-15 | 2007-09-19 | 上海医药工业研究院 | Isatis-Root extractive and the purifying method and the medicine combination |
| CN101396382A (en) * | 2007-09-27 | 2009-04-01 | 上海市脑血管病防治研究所 | Composite isatis root orally disintegrating tablet and preparation method thereof |
| CN103134272A (en) * | 2011-11-23 | 2013-06-05 | 天津天士力现代中药资源有限公司 | Radix isatidis extraction drying method |
| CN104288194A (en) * | 2014-10-30 | 2015-01-21 | 黄琴 | Preparation method for compound radix isatidis granules |
| CN105726591A (en) * | 2014-12-11 | 2016-07-06 | 青岛清泉生物科技有限公司 | Method for extracting effective components of isatis roots through aqueous enzymatic method |
| CN104814988A (en) * | 2015-05-20 | 2015-08-05 | 福州闽海药业有限公司 | Preparation method of indigowoad root granules |
| CN105237615A (en) * | 2015-09-25 | 2016-01-13 | 苏州蔻美新材料有限公司 | Method used for simultaneous extraction of radix isatidis polypeptides and polysaccharides |
Non-Patent Citations (4)
| Title |
|---|
| 唐涛等: "《病原微生物学》", 31 January 2005, 远方出版社 * |
| 张文意等: "槐耳/板蓝根双向发酵体系物质基础变化", 《中国实验方剂学杂志》 * |
| 张明海: "《吃好每天3顿饭》", 28 February 2013, 天津科学技术出版社 * |
| 王大龙: "《小苏打健康生活书》", 31 January 2008, 吉林科学技术出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109010407A (en) * | 2018-09-11 | 2018-12-18 | 华润三九（雅安）药业有限公司 | A kind of Radix Isatidis syrup and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102060936B (en) | Method for extracting rice bran polysaccharide from sub-critical water | |
| CN101779705B (en) | Production method of grape seed oil | |
| CN103724442B (en) | A kind of preparation method of low albumen high-purity Stichopus japonicus polysaccharide | |
| CN102453601B (en) | Extraction method of Cinnamomum camphora seed oil by ultrasound-assisted compound enzyme | |
| CN108865415A (en) | A kind of extracting method of peony seed oil | |
| CN105505558A (en) | Method for synchronously extracting soybean oil, soybean milk powder and soybean dietary fiber by aqueous enzymatic method | |
| CN103214533A (en) | Method for continuously preparing cordycepin and cordyceps polysaccharide by using membrane separation technology | |
| CN104788511A (en) | Method for preparing persimmon tannins with high micromolecular tannin content from persimmons | |
| CN101775233A (en) | Membrane separation integrated technology-based preparation method for producing deodorized red radish pigment | |
| CN103361179A (en) | Extraction method of lemongrass essential oil | |
| CN102558377A (en) | Preparation method of soybean polysaccharide gum | |
| CN105777929A (en) | Extracting method for pleurotus eryngii polysaccharide | |
| CN107349247A (en) | A kind of Radix Isatidis Optimized Extraction Process | |
| CN104447384B (en) | Instant capsicim efficient cryogenic extraction process | |
| CN103864956B (en) | A kind of method extracting pectin from eggplant fruit | |
| CN103349850B (en) | A kind of method for extracting active ingredient in wax-apple | |
| CN102071180A (en) | New process for preparing pineapple bromelain | |
| CN102180991B (en) | Comprehensive utilization method of pumpkin | |
| CN111150758B (en) | Method for continuously preparing polyphenol, polysaccharide and dietary fiber from lotus rhizome nodes | |
| CN103766915A (en) | Processing technology for high-content polysaccharide and O-acetyl aloe dry powder product | |
| CN101366727B (en) | Method for preparing calf blood protein-removed extract and products thereby | |
| CN108252143B (en) | Method for extracting plant salt and cellulose from suaeda salsa | |
| CN102669523A (en) | Method for preparing functional soybean oligosaccharides | |
| CN104857108A (en) | Method for preparing hawthorn extractive through continuous countercurrent ultrasonic equipment | |
| CN107082791A (en) | A kind of method that phenylethanoid glycosides are extracted from saline cistanche |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171117 |