CN106748565A - A kind of production method of complex microorganism calcium and magnesium sulphur silicon fertilizer - Google Patents
A kind of production method of complex microorganism calcium and magnesium sulphur silicon fertilizer Download PDFInfo
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- CN106748565A CN106748565A CN201710176255.7A CN201710176255A CN106748565A CN 106748565 A CN106748565 A CN 106748565A CN 201710176255 A CN201710176255 A CN 201710176255A CN 106748565 A CN106748565 A CN 106748565A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a kind of production method of complex microorganism calcium and magnesium sulphur silicon fertilizer, improvement bacillus subtilis is inserted in sugar residue and is well mixed prepared compound A, after fermentation 2 days, first time turning is carried out, and adds serpentine power, ardealite, silicate bacteria, poly- carbon enzyme, alkali protease, improvement rhizobium that compound B is obtained；Second turning is carried out after 4 days, continues to ferment, temperature reaches after 50 60 DEG C, stirred once every 23 days, obtained final product after the completion of fermentation；Each raw material is respectively according to weight portion：0.1 5 parts of bacillus subtilis of improvement, 12 35 parts of sugar residue, 30 55 parts of serpentine power, 40 75 parts of ardealite, 0.1 4 parts of poly- carbon enzyme, 0.1 2 parts of alkali protease, 0.5 5 parts of rhizobium of improvement.Fertilizer is set to contain substantial amounts of microorganism and calcium and magnesium sulphur silicon etc. using complex microorganism calcium and magnesium sulphur silicon fertilizer obtained in present invention process, comprehensive nutrition, can adjust the acid-base balance of soil, improve soil activation, to increasing crop yield, and improve fruit quality and improve its mouthfeel.
Description
Technical field
The present invention relates to technical field of fertilizers, specifically a kind of production method of complex microorganism calcium and magnesium sulphur silicon fertilizer.
Background technology
In recent decades, the chemicals such as chemical fertilizer, chemical pesticide, chemical herbicide largely are applied in agricultural production
Product.So that soil physical chemistry performance variation, soil hardening, vigor reduction, pollution environment, crops quality decline, remain it is exceeded, and
And the utilization rate of chemical fertilizer, agricultural chemicals is reduced year by year, its main cause is exactly that soil with organic matter is constantly reduced, and can stimulate plant growth
Largely reduced with the beneficial microbe that can improve crop disease-resistant ability, to change this present situation, it is necessary to being added with soil
Machine matter and beneficial microbe, therefore microbial manure just arises at the historic moment.In order to meet development high-efficiency agriculture and nuisanceless green agriculture
The demand of industry, it is proposed that composite microbiological fertilizer.Composite microbiological fertilizer refers to specified microorganisms (phosphorus decomposing, potassium decomposing, the micro- life of fixed nitrogen
Thing) or other by identification it is two or more mutually microorganisms of antagonism be not composited with nutriment, can provide, keep or change
Kind plant nutrient, improves agricultural output or improves the living microorganism product of quality of agricultural product.But existing, crops are applied
Fertilizer of the microbial manure based on nitrogenous, phosphorus and potassium, referred to as " macroelement fertilizer ", and " middle trace element " contains
The fertilizer of calcium, magnesium, sulphur, zinc, iron etc. is then often ignored.And traditional Crop fertilizer presence cannot meet crops to battalion
The need for supporting element, environment is polluted, utilization rate is low, the problem of effect of increasing production difference.
The content of the invention
It is an object of the invention to provide a kind of production method of complex microorganism calcium and magnesium sulphur silicon fertilizer, to solve above-mentioned background
The problem proposed in technology.
To achieve the above object, the present invention provides following technical scheme：
A kind of production method of complex microorganism calcium and magnesium sulphur silicon fertilizer, comprises the following steps：
1) improvement bacillus subtilis is inserted in sugar residue and is well mixed prepared compound A, the water content of regulation compound A
40-60%；
2) fermentation 2 days after, compound A carries out first time turning, and be uniformly added into compound A serpentine power,
Ardealite, silicate bacteria, poly- carbon enzyme, alkali protease, improvement rhizobium are obtained compound B, compound B and first time turning
Carry out simultaneously；
3) second turning being carried out after 4 days, compound B being dissipated to stack and is continued to ferment 15-30 days, temperature reaches 50-60
After DEG C, compound B was stirred every 2-3 days once, complex microorganism calcium and magnesium sulphur silicon fertilizer is obtained final product after the completion of fermentation；
Wherein, each raw material is respectively according to weight portion：0.1-5 parts of bacillus subtilis of improvement, sugar residue 12-35 parts, snake
30-55 parts of stone ore powder, ardealite 40-75 parts, 0.1-4 parts of poly- carbon enzyme, alkali protease 0.1-2 parts, improvement rhizobium 0.5-5
Part.
As further scheme of the invention：Step 1) in, regulation compound A makes its pH value 4-6.
As further scheme of the invention：Step 3) in, compound B was stirred every 2-3 days once, and control temperature
At 60-70 DEG C.
As further scheme of the invention：Step 3) in, moisture content of material is controlled in ＜ 25% after the completion of fermentation.
As further scheme of the invention：Each raw material is respectively according to weight portion：Improvement bacillus subtilis 0.1-3
Part, sugar residue 15-30 parts, serpentine power 35-50 parts, ardealite 45-70 parts, 0.1-2 parts of poly- carbon enzyme, alkali protease 0.1-1
Part, 0.5-3 parts of rhizobium of improvement.
As further scheme of the invention：Each raw material is respectively according to weight portion：Improvement bacillus subtilis 0.2-2
Part, sugar residue 18-28 parts, serpentine power 38-48 parts, ardealite 48-65 parts, 0.2-1.5 parts of poly- carbon enzyme, alkali protease 0.2-
0.8 part, 0.8-2.5 parts of rhizobium of improvement.
As further scheme of the invention：Improve the improved, process of bacillus subtilis：Take activity withered grass gemma high
Bacillus species 10000U/mg, is uniformly divided into two parts：(1) a Bacillus subtilis strain is put into high-temperature cabinet, and temperature is from 30 DEG C
Temperature is slowly heightened, half an hour is adjusted once, and 5 DEG C are heightened every time, and withered grass bud is taken out after temperature is adjusted to 60~70 DEG C of half an hour
Spore bacillus species, observe the Bacillus subtilis strain ratio of survival；The Bacillus subtilis strain of survival is placed incubator
Carry out ectogenesis；(2) another Bacillus subtilis strain is put into household freezer, and temperature slowly turns down temperature, half from 30 DEG C
Hour is adjusted once, and 5 DEG C are turned down every time, and Bacillus subtilis strain is taken out after temperature is adjusted to 0~-10 DEG C of half an hour, and observation is deposited
Bacillus subtilis strain ratio living；It is the Bacillus subtilis strain by high temperature Survival Reproduction and numerous by Survival of low temperature
The Bacillus subtilis strain mixing grown；Then, recycle above-mentioned steps to repeat 300 times, obtain final product high temperature resistant, low temperature resistant change
Good bacillus subtilis.
As further scheme of the invention：Improve the improved, process of rhizobium：Take activity rhizobium strain high
10000U/mg, is uniformly divided into two parts：(1) a rhizobium strain is put into high-temperature cabinet, and temperature slowly heightens temperature from 30 DEG C,
Half an hour is adjusted once, and 5 DEG C are heightened every time, and rhizobium strain, observation survival are taken out after temperature is adjusted to 60~70 DEG C of half an hour
Rhizobium strain ratio；The rhizobium strain of survival is placed incubator carries out ectogenesis；(2) another rhizobium strain
Household freezer is put into, temperature slowly turns down temperature from 30 DEG C, and half an hour adjusts once, 5 DEG C is turned down every time, when temperature is adjusted to 0~-10
Rhizobium strain is taken out after DEG C half an hour, the rhizobium strain ratio of survival is observed；The rhizobium by high temperature Survival Reproduction
Strain and the rhizobium strain bred by Survival of low temperature mix；Then, recycle above-mentioned steps to repeat 300 times, obtain final product resistance to height
Warm, low temperature resistant improvement rhizobium.
Compared with prior art, the beneficial effects of the invention are as follows：
Fertilizer is set to contain substantial amounts of microorganism and calcium using complex microorganism calcium and magnesium sulphur silicon fertilizer obtained in present invention process
Magnesium sulphur silicon etc., comprehensive nutrition and more stable greatly improves the plant nutrient ingredient of available state with sharp plant absorption, can adjust soil
The acid-base balance of earth, improves soil activation, not only can reach the purpose to increasing crop yield, and greatly improve fruit
Quality and its mouthfeel of improvement, while being also greatly improved the disease resistance and lodging resistance of plant, improve utilization rate of fertilizer, accelerate to plant
Thing biochemical reactions speed and material are accumulated, and promote fruit to do sth. in advance maturation.Present invention process is simple, easy to operate, production cost
It is low, it is suitable to large-scale production.
Specific embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described,
Obviously, described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, all
Belong to the scope of protection of the invention.
Embodiment 1
In the embodiment of the present invention, a kind of production method of complex microorganism calcium and magnesium sulphur silicon fertilizer is comprised the following steps：
1) improvement bacillus subtilis is inserted in sugar residue and is well mixed prepared compound A, the water content of regulation compound A
40%, pH value 4.Improve the improved, process of bacillus subtilis：Activity Bacillus subtilis strain 10000U/mg high is taken,
Even is divided into two parts：A Bacillus subtilis strain is put into high-temperature cabinet, and temperature slowly heightens temperature from 30 DEG C, and half an hour adjusts
Once, 5 DEG C are heightened every time, Bacillus subtilis strain is taken out after temperature is adjusted to 60 DEG C of half an hour, observe the withered grass bud of survival
Spore bacillus species ratio；The Bacillus subtilis strain of survival is placed incubator carries out ectogenesis；Another withered grass gemma
Bacillus species are put into household freezer, and temperature slowly turns down temperature from 30 DEG C, and half an hour is adjusted once, and 5 DEG C are turned down every time, when temperature is adjusted
Bacillus subtilis strain is taken out after to 0 DEG C of half an hour, the Bacillus subtilis strain ratio of survival is observed；By high gentle
The Bacillus subtilis strain of breeding living and the Bacillus subtilis strain bred by Survival of low temperature mix；Then, recycle
Above-mentioned steps are repeated 300 times, obtain final product high temperature resistant, low temperature resistant improvement bacillus subtilis.
2) fermentation 2 days after, compound A carries out first time turning, and be uniformly added into compound A serpentine power,
Ardealite, silicate bacteria, poly- carbon enzyme, alkali protease, improvement rhizobium are obtained compound B, compound B and first time turning
Carry out simultaneously.Improve the improved, process of rhizobium：Activity rhizobium strain 10000U/mg high is taken, two parts are uniformly divided into：One
Part rhizobium strain is put into high-temperature cabinet, and temperature slowly heightens temperature from 30 DEG C, and half an hour is adjusted once, and 5 DEG C are heightened every time, works as temperature
Degree takes out rhizobium strain after being adjusted to 60 DEG C of half an hour, observes the rhizobium strain ratio of survival；The rhizobium strain of survival
Placing incubator carries out ectogenesis；Another rhizobium strain is put into household freezer, and temperature slowly turns down temperature, half from 30 DEG C
Hour is adjusted once, and 5 DEG C are turned down every time, and rhizobium strain is taken out after temperature is adjusted to 0 DEG C of half an hour, observes the rhizobium bacterium of survival
The ratio of kind；The rhizobium strain bred the rhizobium strain by high temperature Survival Reproduction and by Survival of low temperature mixes；Then,
Recycle above-mentioned steps to repeat 300 times, obtain final product high temperature resistant, low temperature resistant improvement rhizobium.
3) second turning being carried out after 4 days, compound B being dissipated to stack and is continued to ferment 15 days, temperature is reached after 50 DEG C,
Compound B was stirred every 2 days once, and control temperature at 60-70 DEG C.Moisture content of material is set to control in ＜ after the completion of fermentation
25%, obtain final product complex microorganism calcium and magnesium sulphur silicon fertilizer.
Wherein, each raw material is respectively according to weight portion：0.1 part of bacillus subtilis of improvement, 12 parts of sugar residue, serpentine power
30 parts, 40 parts of ardealite, 0.1 part of poly- carbon enzyme, 0.1 part of alkali protease, improvement 0.5 part of rhizobium.
Embodiment 2
In the embodiment of the present invention, a kind of production method of complex microorganism calcium and magnesium sulphur silicon fertilizer is comprised the following steps：
1) improvement bacillus subtilis is inserted in sugar residue and is well mixed prepared compound A, the water content of regulation compound A
60%, pH value 6.Improve the improved, process of bacillus subtilis：Activity Bacillus subtilis strain 10000U/mg high is taken,
Even is divided into two parts：A Bacillus subtilis strain is put into high-temperature cabinet, and temperature slowly heightens temperature from 30 DEG C, and half an hour adjusts
Once, 5 DEG C are heightened every time, Bacillus subtilis strain is taken out after temperature is adjusted to 70 DEG C of half an hour, observe the withered grass bud of survival
Spore bacillus species ratio；The Bacillus subtilis strain of survival is placed incubator carries out ectogenesis；Another withered grass gemma
Bacillus species are put into household freezer, and temperature slowly turns down temperature from 30 DEG C, and half an hour is adjusted once, and 5 DEG C are turned down every time, when temperature is adjusted
Bacillus subtilis strain is taken out after to -10 DEG C of half an hour, the Bacillus subtilis strain ratio of survival is observed；By high temperature
The Bacillus subtilis strain of Survival Reproduction and the Bacillus subtilis strain bred by Survival of low temperature mix；Then, it is then sharp
Repeated 300 times with above-mentioned steps, obtain final product high temperature resistant, low temperature resistant improvement bacillus subtilis.
2) fermentation 2 days after, compound A carries out first time turning, and be uniformly added into compound A serpentine power,
Ardealite, silicate bacteria, poly- carbon enzyme, alkali protease, improvement rhizobium are obtained compound B, compound B and first time turning
Carry out simultaneously.Improve the improved, process of rhizobium：Activity rhizobium strain 10000U/mg high is taken, two parts are uniformly divided into：One
Part rhizobium strain is put into high-temperature cabinet, and temperature slowly heightens temperature from 30 DEG C, and half an hour is adjusted once, and 5 DEG C are heightened every time, works as temperature
Degree takes out rhizobium strain after being adjusted to 70 DEG C of half an hour, observes the rhizobium strain ratio of survival；The rhizobium strain of survival
Placing incubator carries out ectogenesis；Another rhizobium strain is put into household freezer, and temperature slowly turns down temperature, half from 30 DEG C
Hour is adjusted once, and 5 DEG C are turned down every time, and rhizobium strain is taken out after temperature is adjusted to -10 DEG C of half an hour, observes the rhizobium of survival
Strain ratio；The rhizobium strain bred the rhizobium strain by high temperature Survival Reproduction and by Survival of low temperature mixes；So
Afterwards, recycle above-mentioned steps to repeat 300 times, obtain final product high temperature resistant, low temperature resistant improvement rhizobium.
3) second turning being carried out after 4 days, compound B being dissipated to stack and is continued to ferment 30 days, temperature is reached after 60 DEG C,
Compound B was stirred every 3 days once, and control temperature at 70 DEG C.Moisture content of material is set to control in ＜ after the completion of fermentation
25%, obtain final product complex microorganism calcium and magnesium sulphur silicon fertilizer.
Wherein, each raw material is respectively according to weight portion：5 parts of bacillus subtilis of improvement, 35 parts of sugar residue, serpentine power 55
Part, 75 parts of ardealite, 4 parts of poly- carbon enzyme, 2 parts of alkali protease, 5 parts of rhizobium of improvement.
Embodiment 3
In the embodiment of the present invention, a kind of production method of complex microorganism calcium and magnesium sulphur silicon fertilizer is comprised the following steps：
1) improvement bacillus subtilis is inserted in sugar residue and is well mixed prepared compound A, the water content of regulation compound A
45%, pH value 4.5.Improve the improved, process of bacillus subtilis：Activity Bacillus subtilis strain 10000U/mg high is taken,
It is uniformly divided into two parts：A Bacillus subtilis strain is put into high-temperature cabinet, and temperature slowly heightens temperature, half an hour from 30 DEG C
Adjust once, 5 DEG C are heightened every time, Bacillus subtilis strain is taken out after temperature is adjusted to 65 DEG C of half an hour, observe the withered grass of survival
Bacillus ratio；The Bacillus subtilis strain of survival is placed incubator carries out ectogenesis；Another withered grass bud
Spore bacillus species are put into household freezer, and temperature slowly turns down temperature from 30 DEG C, and half an hour is adjusted once, and 5 DEG C are turned down every time, works as temperature
Bacillus subtilis strain is taken out after being adjusted to -5 DEG C of half an hour, the Bacillus subtilis strain ratio of survival is observed；Through too high
The Bacillus subtilis strain of warm Survival Reproduction and the Bacillus subtilis strain bred by Survival of low temperature mix；Then, then
Repeated 300 times using above-mentioned steps, obtain final product high temperature resistant, low temperature resistant improvement bacillus subtilis.
2) fermentation 2 days after, compound A carries out first time turning, and be uniformly added into compound A serpentine power,
Ardealite, silicate bacteria, poly- carbon enzyme, alkali protease, improvement rhizobium are obtained compound B, compound B and first time turning
Carry out simultaneously.Improve the improved, process of rhizobium：Activity rhizobium strain 10000U/mg high is taken, two parts are uniformly divided into：One
Part rhizobium strain is put into high-temperature cabinet, and temperature slowly heightens temperature from 30 DEG C, and half an hour is adjusted once, and 5 DEG C are heightened every time, works as temperature
Degree takes out rhizobium strain after being adjusted to 65 DEG C of half an hour, observes the rhizobium strain ratio of survival；The rhizobium strain of survival
Placing incubator carries out ectogenesis；Another rhizobium strain is put into household freezer, and temperature slowly turns down temperature, half from 30 DEG C
Hour is adjusted once, and 5 DEG C are turned down every time, and rhizobium strain is taken out after temperature is adjusted to -5 DEG C of half an hour, observes the rhizobium of survival
Strain ratio；The rhizobium strain bred the rhizobium strain by high temperature Survival Reproduction and by Survival of low temperature mixes；So
Afterwards, recycle above-mentioned steps to repeat 300 times, obtain final product high temperature resistant, low temperature resistant improvement rhizobium.
3) second turning being carried out after 4 days, compound B being dissipated to stack and is continued to ferment 20 days, temperature is reached after 55 DEG C,
Compound B was stirred every 2 days once, and control temperature at 60-70 DEG C.Moisture content of material is set to control in ＜ after the completion of fermentation
25%, obtain final product complex microorganism calcium and magnesium sulphur silicon fertilizer.
Wherein, each raw material is respectively according to weight portion：0.1 part of bacillus subtilis of improvement, 15 parts of sugar residue, serpentine power
35 parts, 45 parts of ardealite, 0.1 part of poly- carbon enzyme, 0.1 part of alkali protease, improvement 0.5 part of rhizobium.
Embodiment 4
In the embodiment of the present invention, a kind of production method of complex microorganism calcium and magnesium sulphur silicon fertilizer is comprised the following steps：
1) improvement bacillus subtilis is inserted in sugar residue and is well mixed prepared compound A, the water content of regulation compound A
55%, pH value 5.5.Improve the improved, process of bacillus subtilis：Activity Bacillus subtilis strain 10000U/mg high is taken,
It is uniformly divided into two parts：A Bacillus subtilis strain is put into high-temperature cabinet, and temperature slowly heightens temperature, half an hour from 30 DEG C
Adjust once, 5 DEG C are heightened every time, Bacillus subtilis strain is taken out after temperature is adjusted to 65 DEG C of half an hour, observe the withered grass of survival
Bacillus ratio；The Bacillus subtilis strain of survival is placed incubator carries out ectogenesis；Another withered grass bud
Spore bacillus species are put into household freezer, and temperature slowly turns down temperature from 30 DEG C, and half an hour is adjusted once, and 5 DEG C are turned down every time, works as temperature
Bacillus subtilis strain is taken out after being adjusted to -5 DEG C of half an hour, the Bacillus subtilis strain ratio of survival is observed；Through too high
The Bacillus subtilis strain of warm Survival Reproduction and the Bacillus subtilis strain bred by Survival of low temperature mix；Then, then
Repeated 300 times using above-mentioned steps, obtain final product high temperature resistant, low temperature resistant improvement bacillus subtilis.
2) fermentation 2 days after, compound A carries out first time turning, and be uniformly added into compound A serpentine power,
Ardealite, silicate bacteria, poly- carbon enzyme, alkali protease, improvement rhizobium are obtained compound B, compound B and first time turning
Carry out simultaneously.Improve the improved, process of rhizobium：Activity rhizobium strain 10000U/mg high is taken, two parts are uniformly divided into：One
Part rhizobium strain is put into high-temperature cabinet, and temperature slowly heightens temperature from 30 DEG C, and half an hour is adjusted once, and 5 DEG C are heightened every time, works as temperature
Degree takes out rhizobium strain after being adjusted to 65 DEG C of half an hour, observes the rhizobium strain ratio of survival；The rhizobium strain of survival
Placing incubator carries out ectogenesis；Another rhizobium strain is put into household freezer, and temperature slowly turns down temperature, half from 30 DEG C
Hour is adjusted once, and 5 DEG C are turned down every time, and rhizobium strain is taken out after temperature is adjusted to -5 DEG C of half an hour, observes the rhizobium of survival
Strain ratio；The rhizobium strain bred the rhizobium strain by high temperature Survival Reproduction and by Survival of low temperature mixes；So
Afterwards, recycle above-mentioned steps to repeat 300 times, obtain final product high temperature resistant, low temperature resistant improvement rhizobium.
3) second turning being carried out after 4 days, compound B being dissipated to stack and is continued to ferment 25 days, temperature is reached after 55 DEG C,
Compound B was stirred every 2 days once, and control temperature at 65 DEG C.Moisture content of material is set to control in ＜ after the completion of fermentation
25%, obtain final product complex microorganism calcium and magnesium sulphur silicon fertilizer.
Wherein, each raw material is respectively according to weight portion：3 parts of bacillus subtilis of improvement, 30 parts of sugar residue, serpentine power 50
Part, 70 parts of ardealite, 2 parts of poly- carbon enzyme, 1 part of alkali protease, 3 parts of rhizobium of improvement.
Embodiment 5
In the embodiment of the present invention, a kind of production method of complex microorganism calcium and magnesium sulphur silicon fertilizer is comprised the following steps：
1) improvement bacillus subtilis is inserted in sugar residue and is well mixed prepared compound A, the water content of regulation compound A
50%, pH value 5.Improve the improved, process of bacillus subtilis：Activity Bacillus subtilis strain 10000U/mg high is taken,
Even is divided into two parts：A Bacillus subtilis strain is put into high-temperature cabinet, and temperature slowly heightens temperature from 30 DEG C, and half an hour adjusts
Once, 5 DEG C are heightened every time, Bacillus subtilis strain is taken out after temperature is adjusted to 60 DEG C of half an hour, observe the withered grass bud of survival
Spore bacillus species ratio；The Bacillus subtilis strain of survival is placed incubator carries out ectogenesis；Another withered grass gemma
Bacillus species are put into household freezer, and temperature slowly turns down temperature from 30 DEG C, and half an hour is adjusted once, and 5 DEG C are turned down every time, when temperature is adjusted
Bacillus subtilis strain is taken out after to 0 DEG C of half an hour, the Bacillus subtilis strain ratio of survival is observed；By high gentle
The Bacillus subtilis strain of breeding living and the Bacillus subtilis strain bred by Survival of low temperature mix；Then, recycle
Above-mentioned steps are repeated 300 times, obtain final product high temperature resistant, low temperature resistant improvement bacillus subtilis.
2) fermentation 2 days after, compound A carries out first time turning, and be uniformly added into compound A serpentine power,
Ardealite, silicate bacteria, poly- carbon enzyme, alkali protease, improvement rhizobium are obtained compound B, compound B and first time turning
Carry out simultaneously.Improve the improved, process of rhizobium：Activity rhizobium strain 10000U/mg high is taken, two parts are uniformly divided into：One
Part rhizobium strain is put into high-temperature cabinet, and temperature slowly heightens temperature from 30 DEG C, and half an hour is adjusted once, and 5 DEG C are heightened every time, works as temperature
Degree takes out rhizobium strain after being adjusted to 60 DEG C of half an hour, observes the rhizobium strain ratio of survival；The rhizobium strain of survival
Placing incubator carries out ectogenesis；Another rhizobium strain is put into household freezer, and temperature slowly turns down temperature, half from 30 DEG C
Hour is adjusted once, and 5 DEG C are turned down every time, and rhizobium strain is taken out after temperature is adjusted to 0 DEG C of half an hour, observes the rhizobium bacterium of survival
The ratio of kind；The rhizobium strain bred the rhizobium strain by high temperature Survival Reproduction and by Survival of low temperature mixes；Then,
Recycle above-mentioned steps to repeat 300 times, obtain final product high temperature resistant, low temperature resistant improvement rhizobium.
3) second turning being carried out after 4 days, compound B being dissipated to stack and is continued to ferment 20 days, temperature is reached after 50 DEG C,
Compound B was stirred every 2 days once, and control temperature at 60-70 DEG C.Moisture content of material is set to control in ＜ after the completion of fermentation
25%, obtain final product complex microorganism calcium and magnesium sulphur silicon fertilizer.
Wherein, each raw material is respectively according to weight portion：0.2 part of bacillus subtilis of improvement, 18 parts of sugar residue, serpentine power
38 parts, 48 parts of ardealite, 0.2 part of poly- carbon enzyme, 0.2 part of alkali protease, improvement 0.8 part of rhizobium.
Embodiment 6
In the embodiment of the present invention, a kind of production method of complex microorganism calcium and magnesium sulphur silicon fertilizer is comprised the following steps：
1) improvement bacillus subtilis is inserted in sugar residue and is well mixed prepared compound A, the water content of regulation compound A
50%, pH value 5.Improve the improved, process of bacillus subtilis：Activity Bacillus subtilis strain 10000U/mg high is taken,
Even is divided into two parts：A Bacillus subtilis strain is put into high-temperature cabinet, and temperature slowly heightens temperature from 30 DEG C, and half an hour adjusts
Once, 5 DEG C are heightened every time, Bacillus subtilis strain is taken out after temperature is adjusted to 70 DEG C of half an hour, observe the withered grass bud of survival
Spore bacillus species ratio；The Bacillus subtilis strain of survival is placed incubator carries out ectogenesis；Another withered grass gemma
Bacillus species are put into household freezer, and temperature slowly turns down temperature from 30 DEG C, and half an hour is adjusted once, and 5 DEG C are turned down every time, when temperature is adjusted
Bacillus subtilis strain is taken out after to -10 DEG C of half an hour, the Bacillus subtilis strain ratio of survival is observed；By high temperature
The Bacillus subtilis strain of Survival Reproduction and the Bacillus subtilis strain bred by Survival of low temperature mix；Then, it is then sharp
Repeated 300 times with above-mentioned steps, obtain final product high temperature resistant, low temperature resistant improvement bacillus subtilis.
2) fermentation 2 days after, compound A carries out first time turning, and be uniformly added into compound A serpentine power,
Ardealite, silicate bacteria, poly- carbon enzyme, alkali protease, improvement rhizobium are obtained compound B, compound B and first time turning
Carry out simultaneously.Improve the improved, process of rhizobium：Activity rhizobium strain 10000U/mg high is taken, two parts are uniformly divided into：One
Part rhizobium strain is put into high-temperature cabinet, and temperature slowly heightens temperature from 30 DEG C, and half an hour is adjusted once, and 5 DEG C are heightened every time, works as temperature
Degree takes out rhizobium strain after being adjusted to 70 DEG C of half an hour, observes the rhizobium strain ratio of survival；The rhizobium strain of survival
Placing incubator carries out ectogenesis；Another rhizobium strain is put into household freezer, and temperature slowly turns down temperature, half from 30 DEG C
Hour is adjusted once, and 5 DEG C are turned down every time, and rhizobium strain is taken out after temperature is adjusted to -10 DEG C of half an hour, observes the rhizobium of survival
Strain ratio；The rhizobium strain bred the rhizobium strain by high temperature Survival Reproduction and by Survival of low temperature mixes；So
Afterwards, recycle above-mentioned steps to repeat 300 times, obtain final product high temperature resistant, low temperature resistant improvement rhizobium.
3) second turning being carried out after 4 days, compound B being dissipated to stack and is continued to ferment 25 days, temperature is reached after 60 DEG C,
Compound B was stirred every 3 days once, and control temperature at 60-70 DEG C.Moisture content of material is set to control in ＜ after the completion of fermentation
25%, obtain final product complex microorganism calcium and magnesium sulphur silicon fertilizer.
Wherein, each raw material is respectively according to weight portion：2 parts of bacillus subtilis of improvement, 28 parts of sugar residue, serpentine power 48
Part, 65 parts of ardealite, 1.5 parts of poly- carbon enzyme, 0.8 part of alkali protease, 2.5 parts of rhizobium of improvement.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be in other specific forms realized.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires to be limited rather than described above, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each implementation method is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should
Specification an as entirety, the technical scheme in each embodiment can also be formed into those skilled in the art through appropriately combined
May be appreciated other embodiment.
Claims (8)
1. a kind of production method of complex microorganism calcium and magnesium sulphur silicon fertilizer, it is characterised in that comprise the following steps：
1) improvement bacillus subtilis is inserted in sugar residue and is well mixed prepared compound A, the water content 40- of regulation compound A
60%；
2) after fermentation 2 days, compound A carries out first time turning, and serpentine power, phosphorus stone are uniformly added into compound A
Cream, silicate bacteria, poly- carbon enzyme, alkali protease, improvement rhizobium are obtained compound B, compound B with first time turning simultaneously
Carry out；
3) carry out second turning after 4 days, by compound B dissipate stack continue ferment 15-30 days, temperature reach 50-60 DEG C with
Afterwards, stirred compound B once every 2-3 days, complex microorganism calcium and magnesium sulphur silicon fertilizer is obtained final product after the completion of fermentation；
Wherein, each raw material is respectively according to weight portion：0.1-5 parts of bacillus subtilis of improvement, sugar residue 12-35 parts, serpentine ore
Powder 30-55 parts, ardealite 40-75 parts, 0.1-4 parts of poly- carbon enzyme, alkali protease 0.1-2 parts, improvement 0.5-5 parts of rhizobium.
2. the production method of complex microorganism calcium and magnesium sulphur silicon fertilizer according to claim 1, it is characterised in that step 1) in,
Regulation compound A makes its pH value 4-6.
3. the production method of complex microorganism calcium and magnesium sulphur silicon fertilizer according to claim 1, it is characterised in that step 3) in,
Compound B was stirred every 2-3 days once, and controls temperature at 60-70 DEG C.
4. the production method of complex microorganism calcium and magnesium sulphur silicon fertilizer according to claim 1, it is characterised in that step 3) in,
Moisture content of material is set to control in ＜ 25% after the completion of fermentation.
5. the production method of complex microorganism calcium and magnesium sulphur silicon fertilizer according to claim 1, it is characterised in that each raw material according to
Weight portion is respectively：0.1-3 parts of bacillus subtilis of improvement, sugar residue 15-30 parts, serpentine power 35-50 parts, ardealite 45-
70 parts, 0.1-2 parts of poly- carbon enzyme, alkali protease 0.1-1 parts, improvement 0.5-3 parts of rhizobium.
6. the production method of complex microorganism calcium and magnesium sulphur silicon fertilizer according to claim 5, it is characterised in that each raw material according to
Weight portion is respectively：0.2-2 parts of bacillus subtilis of improvement, sugar residue 18-28 parts, serpentine power 38-48 parts, ardealite 48-
65 parts, 0.2-1.5 parts of poly- carbon enzyme, alkali protease 0.2-0.8 parts, improvement 0.8-2.5 parts of rhizobium.
7. the production method of complex microorganism calcium and magnesium sulphur silicon fertilizer according to claim 1, it is characterised in that improvement withered grass bud
The improved, process of spore bacillus：Activity Bacillus subtilis strain 10000U/mg high is taken, two parts are uniformly divided into：(1) it is a withered
Careless Bacillus are put into high-temperature cabinet, and temperature slowly heightens temperature from 30 DEG C, and half an hour is adjusted once, and 5 DEG C are heightened every time, when
Temperature takes out Bacillus subtilis strain after being adjusted to 60~70 DEG C of half an hour, observes the Bacillus subtilis strain ratio of survival；
The Bacillus subtilis strain of survival is placed incubator carries out ectogenesis；(2) another Bacillus subtilis strain is put into
Household freezer, temperature slowly turns down temperature from 30 DEG C, and half an hour adjusts once, 5 DEG C is turned down every time, when temperature is adjusted to 0~-10 DEG C half
Bacillus subtilis strain is taken out after hour, the Bacillus subtilis strain ratio of survival is observed；By high temperature Survival Reproduction
Bacillus subtilis strain and by Survival of low temperature breed Bacillus subtilis strain mix；Then, above-mentioned step is recycled
It is rapid to repeat 300 times, obtain final product high temperature resistant, low temperature resistant improvement bacillus subtilis.
8. the production method of complex microorganism calcium and magnesium sulphur silicon fertilizer according to claim 1, it is characterised in that improvement rhizobium
Improved, process：Activity rhizobium strain 10000U/mg high is taken, two parts are uniformly divided into：(1) a rhizobium strain is put into
High-temperature cabinet, temperature slowly heightens temperature from 30 DEG C, and half an hour adjusts once, 5 DEG C is heightened every time, when temperature is adjusted to 60~70 DEG C half
Rhizobium strain is taken out after hour, the rhizobium strain ratio of survival is observed；The rhizobium strain of survival is placed incubator to enter
Row ectogenesis；(2) another rhizobium strain is put into household freezer, and temperature slowly turns down temperature from 30 DEG C, and half an hour adjusts one
It is secondary, 5 DEG C are turned down every time, rhizobium strain is taken out after temperature is adjusted to 0~-10 DEG C of half an hour, observe the rhizobium strain of survival
Ratio；The rhizobium strain bred the rhizobium strain by high temperature Survival Reproduction and by Survival of low temperature mixes；Then, then
Repeated 300 times using above-mentioned steps, obtain final product high temperature resistant, low temperature resistant improvement rhizobium.
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