CN106747681A - A kind of method of preparing biological antibacterial organic fertilizer by using immune induced biological technology - Google Patents
A kind of method of preparing biological antibacterial organic fertilizer by using immune induced biological technology Download PDFInfo
- Publication number
- CN106747681A CN106747681A CN201510803007.1A CN201510803007A CN106747681A CN 106747681 A CN106747681 A CN 106747681A CN 201510803007 A CN201510803007 A CN 201510803007A CN 106747681 A CN106747681 A CN 106747681A
- Authority
- CN
- China
- Prior art keywords
- natural
- biological
- instar larva
- organic fertilizer
- immune
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Landscapes
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of method of preparing biological antibacterial organic fertilizer by using immune induced biological technology.There is superpower immune protein synthesis capability present invention utilizes silkworm, sericin antibacterial peptide is synthesized by natural fifth instar larva middle division of silkgland a large amount, synthesize fibroin albumen antibacterial peptide in posterior division of silkgland, Silk gene is regulated and controled with biological high reactivity reagent, with solid phase or the synthesis of liquid phase biotechnology, amplification, biological antibiotic organic fertilizer is obtained.The inventive method biotechnology synthetic route is simple and convenient and easy to study, low production cost, and yield degree of controllability is high, and disease prevention ability is up to more than 80% ~ 95%, and achievement is easily converted, is easy to industrialization production；Soil is mixed when agricultural using biological antibiotic organic fertilizer obtained in the inventive method, leaching root before planting, the infection caused to the pathogen such as gram-positive bacteria, Gram-negative bacteria or hay bacillus, bacterial wilt, Fusarium wilt, the antibacterial advantage with stronger antibacterial action and efficient, wide spectrum.
Description
Technical field
The present invention relates to a kind of preparation method of biological antibiotic organic fertilizer, more particularly to a kind of method of preparing biological antibacterial organic fertilizer by using immune induced biological technology.
Background technology
The continuous cropping obstacle of the crops such as some melon fruits and vegetables has turned into the bottleneck of restriction agricultural production at present, the items countryside tax system such as land resource and human and material resources, financial resources is caused to waste, agricultural performance is have impact on, while also greatly having hit the enthusiasm for production of peasant.It is main by fungoid disease opportunistic pathogen in the continuous cropping soil obstacle of succession crop（Fusarium oxysporum）The withered basket disease of continuous cropping for causing is the obstruction factor of most serious, and such as withered basket disease of continuous cropping banana Panama begins to appearance for 4 batches in continuous cropping, serious all the more after 6 batches of continuous cropping；By the blue or green withered microbial pepper ralstonia solanacearums of Lao Ershi once occurring to cause the capsicum of 20-30% dead, more than 50%, or even total crop failure are up to when serious.
At present, the preventing and treating to this continuous cropping obstacle mainly has the distinct methods such as chemical prevention, breeding resistant variety, but effect is poor.Correlative study result shows, the key issue of the withered basket disease of succession crop is microbiota and its secondary substance intoxicating problem in soil, therefore, preventing and treating to the obstacle of this kind of succession crop is also gradually transferred to by screening efficient antagonistic strain, while can either be in the comprehensive preventive health measures based on biological control of microbial organic fertilizer that is disease-resistant and can improving soil texture by antagonistic strain and organic fertilizer compounding exploitation.For example, Chinese patent ZL
200510122898.0 disclose a kind of microbial organic fertilizer of the Antagonistic Fungi and its production that can be overcome or eliminate the withered basket disease of succession crop, and its experiment shows that after being manured into soil Antagonistic Fungi can bred rapidly the microbial organic fertilizer, advantage group is formed in soil；Chinese patent ZL
200910183358.1、ZL 200910183361.3、ZL 200910035425.5、ZL
201010138221.7 individually disclose prevent and kill off continuous cropping cucumber rhizoctonia rot, prevent and kill off continuous cropping banana Panama wilt disease, prevent and kill off eliminating greensickness of continuous cropping cotton, preventing and treating pepper ralstonia solanacearum Antagonistic Fungi and its microbial organic fertilizer.
In terms of anti-microbial type bio-pharmaceutical research and development, antibacterial peptide is current research emphasis and focus.Antibacterial peptide (antibacterial peptides) is also known as peptide antibiotic (peptide antibiotics) or antimicrobial (antimicrobialpeptide), it is the active peptides in the extraneous biological infection source of class resistance that Immune System is produced, is widely present in insect, plant, animal and human body.It is in addition to playing the role of nonspecific antibacterium, fungi, virus, anti-infective, anti-disease, and also anti-cell variation, the effect of antitumor cell and does not damage human normal cell, especially still effective to multiple antibiotic resistant strain.Antibacterial peptide has stronger killing action to endurance strains such as the kind of Gram-positive and negative bacteria about more than 40, Pseudomonas aeruginosa and paratyphoid bacterium.
Existing scholar has carried out research, experiment to the worldwide bacterium destructiveness soil-borne disease such as the tomato caused by Stalk Rot and pepper ralstonia solanacearum (Bacterial Wilt), the banana blight (Fusarium wilt) caused by Fusarium wilt, proof utilizes antibacterial peptide, above disease can effectively be prevented and treated by mixing soil, applying the methods such as filling, plant leaching root, seed dressing, especially in the environment of the warm moist such as China Guangdong and Guangxi Provinces and Fujian, the incidence of disease can reduce more than 80%.By the extensive use of biologic antibiotic peptide, can effectively prevent, mitigate agricultural pest, reduce the amount of application of agricultural chemicals, reduce agricultural production input, it is ensured that agricultural product edible safety, realize agricultural production and income, so as to play a part of to get twice the result with half the effort, environmental protection.
On the other hand, correlative study is proved：Silkworm has superpower immune protein synthesis capability, and a silkworm in the growth period less than one month, can synthesize and secrete the silk-fibroin of 0.5 ~ 1.5g using the mulberry leaf of 22 ~ 25g.There is now the correlative study of cultivated silkworm antimicrobial peptide, such as Chinese patent ZL200910116463.3 discloses a kind of method that inducing silkworms produce antibacterial peptide, and described the method is to use virus or bacterial injections, or can use the method for making silkworm hungry；During using virus or bacterial injections, micro bacterium or virus is injected to silkworm, during using the method for making silkworm hungry, mulberry leaf are not fed to silkworm, make it hungry 48 hours, then normally raise 24 hours, abductive approach described in the patent is simple and easy to apply, more antibacterial peptide can be effectively induced, antibacterial peptide extraction efficiency is greatly improved；Chinese patent ZL200910193620.0 then discloses a kind of cultivated silkworm antimicrobial peptide BmcecE and its cDNA sequence and application, the cultivated silkworm antimicrobial peptide of the invention is to gram-positive bacteria (bacillus thuringiensis), and Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, clever bacterium sepsis germ or Ralstonia solanacearum) it is respectively provided with antibacterial activity, has a broad antifungal spectrum, cultivated silkworm antimicrobial peptide BmcecE can be used for people, poultry, the medicament research and development of fowl, prevention and treatment are by bacillus thuringiensis, Escherichia coli, Pseudomonas aeruginosa, clever bacterium sepsis germ or Ralstonia solanacearum infection cause people, poultry, fowl cause a disease, can be also used for the preparation of biological product additive, prevent by bacillus thuringiensis, Escherichia coli, Pseudomonas aeruginosa, the biological product that clever bacterium sepsis germ or Ralstonia solanacearum cause it is putrid and deteriorated.
But have no at present using immune induction biotechnology, synthesize fibroin albumen antibacterial peptide using fifth instar larva middle division of silkgland a large amount synthesis sericin antibacterial peptide, in posterior division of silkgland, and then synthesized by solid phase or liquid phase biotechnology, expanded, prepare the correlative study of the biological antibiotic organic fertilizer for the production of agriculture antibacterial.
The content of the invention
It is an object of the invention to provide a kind of method of preparing biological antibacterial organic fertilizer by using immune induced biological technology.
In order to solve the above problems, the technical solution adopted in the present invention is as follows：A kind of method of preparing biological antibacterial organic fertilizer by using immune induced biological technology, with natural fifth instar larva as raw material, sericin antibacterial peptide is synthesized by natural fifth instar larva middle division of silkgland a large amount, synthesize fibroin albumen antibacterial peptide in posterior division of silkgland, silk-fibroin antifungal genes are regulated and controled with biological high reactivity reagent, with solid phase or the synthesis of liquid phase biotechnology, amplification, biological antibiotic organic fertilizer is obtained, described regulation and control and solid phase or the synthesis of liquid phase biotechnology, amplification are concretely comprised the following steps：Grown into the 3rd day in natural fifth instar larva, when synthesizing sericin or natural fifth instar larva posterior division of silkgland synthesis fibroin albumen at natural fifth instar larva middle division of silkgland 1/2, carry out immune induction, then in immune peak period, by natural fifth instar larva silkworm body it is freeze-dried to moisture be 70% ~ 75% or by natural fifth instar larva silkworm body directly mill crushing after, in input 50kg reactors, and add the biological high reactivity reagent for accounting for natural fifth instar larva silkworm weight 1/1000, ferment 6-8 days at normal temperatures, after lyophilized, that is, obtain biological antibiotic organic fertilizer.
The method of preparing biological antibacterial organic fertilizer by using immune induced biological technology of the present invention is the one kind in following method：
（1）Natural fifth instar larva grows into the 3rd day, when synthesizing sericin at natural fifth instar larva body middle division of silkgland 1/2, hay bacillus is inoculated with inoculator, immune induction is carried out, in immune peak period, it is 70% ~ 75% by natural fifth instar larva silkworm body freeze-drying to moisture, then by freeze-dried natural fifth instar larva silkworm body input 50kg reactors, and the biological high reactivity reagent for accounting for natural fifth instar larva silkworm weight 1/1000 is added, is fermented 6-8 days at normal temperatures, after lyophilized, that is, obtain biological antibiotic organic fertilizer；
（2）Natural fifth instar larva grows into the 3rd day, when synthesizing sericin at natural fifth instar larva body middle division of silkgland 1/2, hay bacillus is inoculated with inoculator, immune induction is carried out, in immune peak period, in the liquid input 50kg reactors crushed and will be obtained after crushing that natural fifth instar larva silkworm body is directly milled, addition accounts for the biological high reactivity reagent of natural fifth instar larva silkworm weight 1/1000, ferment 6-8 days at normal temperatures, after freezing, that is, obtain biological antibiotic organic fertilizer；
（3）Natural fifth instar larva grows into the 3rd day, when synthesizing fibroin albumen at natural fifth instar larva body afterbody sericterium, hay bacillus is inoculated with inoculator, immune induction is carried out, in immune peak period, by natural fifth instar larva silkworm body freeze-drying to moisture 70% ~ 75%, by in freeze-dried natural fifth instar larva silkworm body input 50kg reactors, and the biological high reactivity reagent for accounting for natural fifth instar larva silkworm weight 1/1000 is added, fermented 6-8 days at normal temperatures, after lyophilized, that is, obtain biological antibiotic organic fertilizer；
（4）Natural fifth instar larva grows into the 3rd day, when synthesizing fibroin albumen at natural fifth instar larva body afterbody sericterium, hay bacillus is inoculated with inoculator, immune induction is carried out, in immune peak period, in the liquid input 50kg reactors crushed and will be obtained after crushing that natural fifth instar larva silkworm body is directly milled, addition accounts for the biological high reactivity reagent of natural fifth instar larva silkworm weight 1/1000, ferment 6-8 days at normal temperatures, after freezing, that is, obtain biological antibiotic organic fertilizer.
The immune induction method is：Hay bacillus is inoculated at natural fifth instar larva silkworm body middle division of silkgland 1/2 or is inoculated at natural fifth instar larva silkworm body afterbody sericterium with inoculator, natural fifth instar larva silkworm body on the 3rd ~ 5 reaches immune peak period after inoculation.
The biological high reactivity reagent is：Biological mitogen, is Zhanjiang Normal University's independent intellectual property right achievement in research production.
The lyophilized condition is：In 2-4 hours, temperature is dropped into -20 DEG C of pre-freezing temperature by normal temperature, kept for -20 DEG C 1.5-3 hours, then -5 ~ -8 DEG C are warming up to by -20 DEG C, maintained 2 ~ 4 hours, then drained with vavuum pump.
The biological antibiotic organic fertilizer is solid antimicrobial organic fertilizer or liquid antibiotic organic fertilizer.
The present invention is relative to the beneficial effect of prior art：
（1）The method of the synthetically prepared biological antibiotic organic fertilizer of immune induction biotechnology of the present invention, its biotechnology synthetic route is simple and convenient and easy to study, and low production cost, yield degree of controllability is high, and disease prevention ability is up to more than 95%, and is easy to industrialization production；
（2）The biological antibiotic organic fertilizer prepared using the inventive method mixes soil when agricultural, leaching root before planting, the infection caused to gram-positive bacteria (bacillus thuringiensis) and Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, clever bacterium sepsis germ or Ralstonia solanacearum) or by bacillus thuringiensis, Escherichia coli, Pseudomonas aeruginosa, the clever pathogen such as bacterium sepsis germ or Ralstonia solanacearum, hay bacillus, bacterial wilt, Fusarium wilt, the antibacterial advantage with stronger antibacterial action and efficient, wide spectrum.
Specific embodiment
The present invention is described in further details below by embodiment, these embodiments are only used for illustrating the present invention, do not limit the scope of the invention.
Embodiment 1Using the method for the invention, it follow these steps to prepare a kind of biological antibiotic organic fertilizer for the production of agriculture antibacterial：
（1）Antibody strain：Hay bacillus；Biological high reactivity reagent：From the biological mitogen of Zhanjiang Normal University's independent intellectual property right achievement in research production；
（2）Preparation process：
1. natural fifth instar larva grows into the 3rd day, when synthesizing sericin at natural fifth instar larva body middle division of silkgland 1/2, hay bacillus is inoculated at natural fifth instar larva silkworm body middle division of silkgland 1/2 with inoculator, immune induction, natural fifth instar larva silkworm body on the 5th reaches immune peak period after inoculation；
2. in immune peak period, it is 70% by the natural fifth instar larva silkworm body freeze-drying in immune peak period to moisture, then by freeze-dried natural fifth instar larva silkworm body input 50kg reactors, and the biological mitogen for accounting for natural fifth instar larva silkworm weight 1/1000 is added, ferment 6 days at normal temperatures；
3. fermentation ends, are freezed the product after fermentation using following lyophilisation condition：2.5 hours normal temperature drops to -20 DEG C of pre-freezing temperature, is kept for -20 DEG C 2 hours, then is warming up to -5 DEG C by -20 DEG C, maintains 2 hours, is then drained with vavuum pump；
4. product is solid antimicrobial organic fertilizer finished product after freezing, and agriculture antibacterial production is used for after packing.
Embodiment 2Using the method for the invention, it follow these steps to prepare a kind of biological antibiotic organic fertilizer for the production of agriculture antibacterial：
（1）Antibody strain：Hay bacillus；Biological high reactivity reagent：From the biological mitogen of Zhanjiang Normal University's independent intellectual property right achievement in research production；
（2）Preparation process：
1. natural fifth instar larva grows into the 3rd day, when synthesizing sericin at natural fifth instar larva body middle division of silkgland 1/2, hay bacillus is inoculated at natural fifth instar larva silkworm body middle division of silkgland 1/2 with inoculator, immune induction, natural fifth instar larva silkworm body on the 4th reaches immune peak period after inoculation；
2. in immune peak period, the natural fifth instar larva silkworm body in immune peak period is directly milled in the liquid input 50kg reactors crushed and will be obtained after crushing, addition accounts for the biological mitogen of natural fifth instar larva silkworm weight 1/1000, ferments 6 days at normal temperatures；
3. fermentation ends, are freezed the product after fermentation using following lyophilisation condition：2 hours normal temperature drops to -20 DEG C of pre-freezing temperature, is kept for -20 DEG C 3 hours, then is warming up to -6 DEG C by -20 DEG C, maintains 3 hours, is then drained with vavuum pump；
4. product is liquid antibiotic organic fertilizer finished product after freezing, and agriculture antibacterial production is used for after packing.
Embodiment 3Using the method for the invention, it follow these steps to prepare a kind of biological antibiotic organic fertilizer for the production of agriculture antibacterial：
（1）Target antibody strain：Hay bacillus；Biological high reactivity reagent：From the biological mitogen of Zhanjiang Normal University's independent intellectual property right achievement in research production；
（2）Preparation process：
1. natural fifth instar larva grows into the 3rd day, when synthesizing fibroin albumen at natural fifth instar larva body afterbody sericterium, hay bacillus is inoculated at natural fifth instar larva silkworm body afterbody sericterium with inoculator, immune induction, and natural fifth instar larva silkworm body on the 3rd reaches immune peak period after inoculation；
2. in immune peak period, it is 75% by the natural fifth instar larva silkworm body freeze-drying in immune peak period to moisture, then by freeze-dried natural fifth instar larva silkworm body input 50kg reactors, and the biological mitogen for accounting for natural fifth instar larva silkworm weight 1/1000 is added, ferment 8 days at normal temperatures；
3. fermentation ends, are freezed the product after fermentation using following lyophilisation condition：3 hours normal temperature drops to -20 DEG C of pre-freezing temperature, is kept for -20 DEG C 2.5 hours, then is warming up to -6 DEG C by -20 DEG C, maintains 2 hours, is then drained with vavuum pump；
4. product is solid antimicrobial organic fertilizer finished product after freezing, and agriculture antibacterial production is used for after packing.
Embodiment 4Using the method for the invention, it follow these steps to prepare a kind of biological antibiotic organic fertilizer for the production of agriculture antibacterial：
（1）Antibody strain：Hay bacillus；Biological high reactivity reagent：From the biological mitogen of Zhanjiang Normal University's independent intellectual property right achievement in research production；
（2）Preparation process：
1. natural fifth instar larva grows into the 3rd day, when synthesizing fibroin albumen at natural fifth instar larva body afterbody sericterium, hay bacillus is inoculated at natural fifth instar larva silkworm body afterbody sericterium with inoculator, immune induction, and natural fifth instar larva silkworm body on the 3rd reaches immune peak period after inoculation；
2. in immune peak period, the natural fifth instar larva silkworm body in immune peak period is directly milled in the liquid input 50kg reactors crushed and will be obtained after crushing, addition accounts for the biological mitogen of natural fifth instar larva silkworm weight 1/1000, ferments 7 days at normal temperatures；
3. fermentation ends, are freezed the product after fermentation using following lyophilisation condition：4 hours normal temperature drops to -20 DEG C of pre-freezing temperature, is kept for -20 DEG C 1.5 hours, then is warming up to -8 DEG C by -20 DEG C, maintains 4 hours, is then drained with vavuum pump；
4. product is liquid antibiotic organic fertilizer finished product after freezing, and agriculture antibacterial production is used for after packing.
Claims (6)
1. a kind of method of preparing biological antibacterial organic fertilizer by using immune induced biological technology, it is characterised in that：The method is with natural fifth instar larva as raw material, sericin antibacterial peptide is synthesized by natural fifth instar larva middle division of silkgland a large amount, synthesize fibroin albumen antibacterial peptide in posterior division of silkgland, silk-fibroin antifungal genes are regulated and controled with biological high reactivity reagent, with solid phase or the synthesis of liquid phase biotechnology, amplification, it is obtained biological antibiotic organic fertilizer, described regulation and control and solid phase or the synthesis of liquid phase biotechnology, amplification are concretely comprised the following steps：Grown into the 3rd day in natural fifth instar larva, when synthesizing sericin or natural fifth instar larva posterior division of silkgland synthesis fibroin albumen at natural fifth instar larva middle division of silkgland 1/2, carry out immune induction, then in immune peak period, by natural fifth instar larva silkworm body it is freeze-dried to moisture be 70% ~ 75% or by natural fifth instar larva silkworm body directly mill crushing after, in input 50kg reactors, and add the biological high reactivity reagent for accounting for natural fifth instar larva silkworm weight 1/1000, ferment 6-8 days at normal temperatures, after lyophilized, that is, obtain biological antibiotic organic fertilizer.
2. a kind of method of preparing biological antibacterial organic fertilizer by using immune induced biological technology as claimed in claim 1, it is characterised in that：It is the one kind in following method：
（1）Natural fifth instar larva grows into the 3rd day, when synthesizing sericin at natural fifth instar larva body middle division of silkgland 1/2, hay bacillus is inoculated with inoculator, immune induction is carried out, in immune peak period, it is 70% ~ 75% by natural fifth instar larva silkworm body freeze-drying to moisture, then by freeze-dried natural fifth instar larva silkworm body input 50kg reactors, and the biological high reactivity reagent for accounting for natural fifth instar larva silkworm weight 1/1000 is added, is fermented 6-8 days at normal temperatures, after lyophilized, that is, obtain biological antibiotic organic fertilizer；
（2）Natural fifth instar larva grows into the 3rd day, when synthesizing sericin at natural fifth instar larva body middle division of silkgland 1/2, hay bacillus is inoculated with inoculator, immune induction is carried out, in immune peak period, in the liquid input 50kg reactors crushed and will be obtained after crushing that natural fifth instar larva silkworm body is directly milled, addition accounts for the biological high reactivity reagent of natural fifth instar larva silkworm weight 1/1000, ferment 6-8 days at normal temperatures, after freezing, that is, obtain biological antibiotic organic fertilizer；
（3）Natural fifth instar larva grows into the 3rd day, when synthesizing fibroin albumen at natural fifth instar larva body afterbody sericterium, hay bacillus is inoculated with inoculator, immune induction is carried out, in immune peak period, by natural fifth instar larva silkworm body freeze-drying to moisture 70% ~ 75%, by in freeze-dried natural fifth instar larva silkworm body input 50kg reactors, and the biological high reactivity reagent for accounting for natural fifth instar larva silkworm weight 1/1000 is added, fermented 6-8 days at normal temperatures, after lyophilized, that is, obtain biological antibiotic organic fertilizer；
（4）Natural fifth instar larva grows into the 3rd day, when synthesizing fibroin albumen at natural fifth instar larva body afterbody sericterium, hay bacillus is inoculated with inoculator, immune induction is carried out, in immune peak period, in the liquid input 50kg reactors crushed and will be obtained after crushing that natural fifth instar larva silkworm body is directly milled, addition accounts for the biological high reactivity reagent of natural fifth instar larva silkworm weight 1/1000, ferment 6-8 days at normal temperatures, after freezing, that is, obtain biological antibiotic organic fertilizer.
3. the method for a kind of preparing biological antibacterial organic fertilizer by using immune induced biological technology according to claim 1 and 2, it is characterised in that：The immune induction method is：Hay bacillus is inoculated at natural fifth instar larva silkworm body middle division of silkgland 1/2 or is inoculated at natural fifth instar larva silkworm body afterbody sericterium with inoculator, natural fifth instar larva silkworm body on the 3rd ~ 5 reaches immune peak period after inoculation.
4. the method for a kind of preparing biological antibacterial organic fertilizer by using immune induced biological technology according to claim 1 and 2, it is characterised in that：The biological high reactivity reagent is：Biological mitogen.
5. the method for a kind of preparing biological antibacterial organic fertilizer by using immune induced biological technology according to claim 1 or 2, it is characterised in that：The lyophilized condition is：In 2-4 hours, temperature is dropped into -20 DEG C of pre-freezing temperature by normal temperature, kept for -20 DEG C 1.5-3 hours, then -5 ~ -8 DEG C are warming up to by -20 DEG C, maintained 2 ~ 4 hours, then drained with vavuum pump.
6. the method for a kind of preparing biological antibacterial organic fertilizer by using immune induced biological technology according to claim 1 or 2, it is characterised in that：The biological antibiotic organic fertilizer is solid antimicrobial organic fertilizer or liquid antibiotic organic fertilizer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510803007.1A CN106747681A (en) | 2015-11-19 | 2015-11-19 | A kind of method of preparing biological antibacterial organic fertilizer by using immune induced biological technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510803007.1A CN106747681A (en) | 2015-11-19 | 2015-11-19 | A kind of method of preparing biological antibacterial organic fertilizer by using immune induced biological technology |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106747681A true CN106747681A (en) | 2017-05-31 |
Family
ID=58916700
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510803007.1A Withdrawn CN106747681A (en) | 2015-11-19 | 2015-11-19 | A kind of method of preparing biological antibacterial organic fertilizer by using immune induced biological technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106747681A (en) |
-
2015
- 2015-11-19 CN CN201510803007.1A patent/CN106747681A/en not_active Withdrawn
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104498386B (en) | The preparation method and application of raw Bacillus amyloliquefaciens strain SZ23 and zymotic fluid in wild jujube | |
| CN105347918A (en) | Method for preparing bio-organic fertilizer from walnut green seedcase | |
| CN105724435A (en) | Agricultural insecticidal composition and application thereof | |
| CN106490057A (en) | A kind of composite pesticide for Rice Cropping and preparation method thereof | |
| CN104845624A (en) | Natural plant biological composite nutrient solution for killing nematode and preparation method thereof | |
| KR20150024629A (en) | Medium for cultivating mushrooms comprising medicinal herb and preparation method thereof | |
| KR20190065916A (en) | Medium composition for cultivating vegetable worms and the method of cultivating vegetable worms | |
| KR102251508B1 (en) | Novel Beauveria bassiana KNU-101 Strain with Improved Insecticidal Effect and Spore Production and Uses thereof | |
| CN104855134A (en) | High-yield cultivation method for auricularia polytricha | |
| CN110078806B (en) | Arthropoda-japonica antibacterial peptide AtR100 and application thereof | |
| CN102584452A (en) | Method for preparing biological antibacterial organic fertilizer by using immune induced biological technology | |
| CN105061078A (en) | Health bio-organic fertilizer and preparation method thereof | |
| CN102907458B (en) | Biological mosquitocide and preparation method thereof | |
| CN106397030A (en) | Composite microbial fertilizer and preparation method thereof | |
| CN102613249A (en) | Process for preparing board-spectrum antibiotics by immune induction biotechnology | |
| CN106396836B (en) | Soybean phytophthora root rot prevents and treats biological seed coating agent and preparation method thereof, application method | |
| CN101366394B (en) | Composite biological insecticide | |
| CN103621564B (en) | A kind of preparation method of biological antibiosis agent | |
| CN106747681A (en) | A kind of method of preparing biological antibacterial organic fertilizer by using immune induced biological technology | |
| CN103641631B (en) | Broad-spectrum antibacterial biological fertilizer | |
| CN108690818A (en) | A kind of microbial bacterial agent and preparation method thereof | |
| KR100314323B1 (en) | Bacillus sp. GB-017 KFCC-11070 | |
| JP2009007220A (en) | Anti-virulence and/or insect pest repelling organic fermentative bacterial fertilizer | |
| EP1384405B1 (en) | Bactericidal, bacteriostatic and fungicidal composition comprising two or more live species of trichoderma | |
| CN111543443A (en) | Biological insect-resist agent and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20170531 |
|
| WW01 | Invention patent application withdrawn after publication |