CN106721665A - A kind of Chinese herbal and crude drugs preparations for improving Bostrichthys sinensis immunity - Google Patents
A kind of Chinese herbal and crude drugs preparations for improving Bostrichthys sinensis immunity Download PDFInfo
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- CN106721665A CN106721665A CN201611251143.5A CN201611251143A CN106721665A CN 106721665 A CN106721665 A CN 106721665A CN 201611251143 A CN201611251143 A CN 201611251143A CN 106721665 A CN106721665 A CN 106721665A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention provides a kind of Chinese herbal and crude drugs preparations for improving Bostrichthys sinensis immunity, and preparation process is as follows:Pretreatment:Compound Chinese herb is crushed, is mixed, be made compound Chinese herb powder;Extraction:The pure water for adding compound Chinese herb powder to accumulate 5 ~ 11 times, Ultrasonic Wave-Assisted Extraction;Fermentation:Fermenting mixture is added after extraction, pH value is adjusted, zymotic fluid is made, by zymotic fluid addition fermentation tank, bacillus subtilis and S. cervisiae is inoculated with, aerobic fermentation is inoculated with lactobacillus bulgaricus and bifidobacterium bifidum, anaerobic fermentation;Post processing:Anaerobic fermented liquid is adsorbed with wheat bran, be dry, pulverize, sieved, obtain Chinese herbal and crude drugs preparations.Chinese herbal and crude drugs preparations prepared by the present invention can suppress the harmful bacteria breeding of Bostrichthys sinensis enteron aisle and grow, and effect substantially, can replace antibiotic usage, and noresidue, on the growth of Bostrichthys sinensis without influence, lifts culture benefit.
Description
Technical field
The invention belongs to immunostimulant field, and in particular to a kind of Chinese herbal medicine system of raising Bostrichthys sinensis immunity
Agent.
Background technology
Bostrichthys sinensis belong to Osteichthyes, Perciformes, free time tiger fish suborder, Eleotridae, are commonly called as " snakeheaded fish ", " mudfish ", " soil
Fish " etc., is a kind of water warm shallow sea fish.The salt water region such as coastal areas of southern China, river mouth, bay is distributed, and likes to inhabit
Husky mud substrate or reef area, seek troglodytism.Bostrichthys sinensis meat exquisiteness, delicious flavour, nutritious, economic worth are high,
It is a kind of rare seafood fish.In recent years, the success of Bostrichthys sinensis artificial breeding, is the development of Bostrichthys sinensis aquaculture
Lay a good foundation.
Antibioticses feed addictive is subject to heat to hold in both hands, and is widely used in feed industry, and water is promoted to a certain extent
Produce the development of aquaculture, but antibiotic bring high residue, high pollution and the resistance to the action of a drug the problems such as more serious.Chinese herbal medicine is raised
Feed additives meet green aquatic products aquaculture development requirement, eliminate the safety worries that people feed out aquatic products product to it, value
Application must be widelyd popularize.But the research level of current Chinese herbal feed additive is relatively low, and manufacture craft simple coarse, product science and technology contains
Amount is relatively low, and quality is not sufficiently stable, and such problem seriously governs the popularization and application of Chinese medicine fodder addition.
Prior art such as Authorization Notice No. CN 103609848 B aquatic products fermentation of Chinese herbal medicine feed addictive and its preparation
A kind of method disclosure of the invention aquatic products fermentation of Chinese herbal medicine feed addictive, it is with prevention, health care, treatment and regulation immune
It is the preferable, green, environmentally friendly of substitute antibiotics medicine, safety, novel fodder additive etc. multiple efficacies.The fermentation of Chinese herbal medicine
Feed addictive is obtained by compound Chinese herb by " Chinese herbal medicine Ultrasonic Wave-Assisted Extraction+multi-cultur es two-step fermentation " technique, tool
There is the two-fold advantage of Chinese herbal medicine and probiotics, can efficiently nurse one's health aquatic products animal intestinal tract health, treat both principal and secondary aspect of disease, reinvigoration is protected
Intestinal microecology balance and optimal immune state are held, the digestibility of nutriment is improved, animal body immunity is improved, carried
Height cultivation income, reduces cultivation risk, and substitute antibiotics use, aquatic livestock is had no adverse effects, using safety, in the absence of medicine
The problems such as thing residual and drug resistance, there are wide market prospects, but suppressing the flourish side of aquatic products harmful intestinal tract bacteria
Face need to be improved, and ferment effect also has room for promotion.
The content of the invention
The present invention provides that a kind of phagostimulating effect is good, can effectively suppress Bostrichthys sinensis harmful intestinal tract for above-mentioned technical problem
Bacteria growing and breeding, beneficial to cultivation immunity is improved, the excellent one kind of ferment effect improves Bostrichthys sinensis in preparation process
The Chinese herbal and crude drugs preparations of immunity.
It is of the invention to be for the scheme that above-mentioned technical problem is taken:A kind of Chinese herbal medicine for improving Bostrichthys sinensis immunity
Preparation, preparation process is as follows:
1)Pretreatment:Compound Chinese herb is crushed, is mixed, be made compound Chinese herb powder;
2)Extraction:The pure water for adding compound Chinese herb powder to accumulate 5 ~ 11 times, Ultrasonic Wave-Assisted Extraction;
3)Fermentation:Fermenting mixture is added after extraction, pH value is adjusted, zymotic fluid is made, by zymotic fluid addition fermentation tank, is inoculated with
Bacillus subtilis and S. cervisiae, aerobic fermentation are inoculated with lactobacillus bulgaricus and bifidobacterium bifidum, anaerobic fermentation;
4)Post processing:Anaerobic fermented liquid is adsorbed with wheat bran, be dry, pulverize, sieved, obtain Chinese herbal and crude drugs preparations.
Compound Chinese herb is crushed to 40 ~ 70 mesh in step 1, extracting effect is lifted, by the beneficiating ingredient extraction in herbal medicine
Out.
In step 2 under ultrasonic assistant, 20 ~ 30min is extracted under the conditions of 90 ~ 110 DEG C, lift extracting effect, improve leaching
Soluble beneficiating ingredient content in extract.
The zymotic fluid of fermentation tankage size 60 ~ 71% is added in step 3, bacillus subtilis and S. cervisiae, inoculation is inoculated with
Amount is respectively 2 ~ 4%, 4.8 ~ 8%, and it is 27 ~ 37 DEG C to control fermentation temperature, 150 ~ 300rpm of rotating speed, 10 ~ 20L/min of throughput, hair
12 ~ 24h of ferment, after fermentation ends, is inoculated with lactobacillus bulgaricus and bifidobacterium bifidum, and inoculum concentration is 2 ~ 4%, and fermentation temperature is
28 ~ 37 DEG C, ferment 12 ~ 24h.The active polysaccharide in Chinese herbal and crude drugs preparations is improved, beneficial to the immunity and product that improve Bostrichthys sinensis
Matter.
Bacillus subtilis(Chinese agriculture Microbiological Culture Collection administrative center, bacterium numbering ACCC10619), wine brewing
Saccharomycete (Angel Yeast), lactobacillus bulgaricus(Chinese agriculture Microbiological Culture Collection administrative center, bacterium numbering
ACCC10638)And bifidobacterium bifidum(ATCC standard strains, bacterium numbering ATCC29521)
Wheat bran volume is 1 ~ 2 times of fermentating liquid volume in step 4, beneficiating ingredient in zymotic fluid can be preferably absorbed, at 40 ~ 60 DEG C
Under the conditions of dry, moisture control 8 ~ 10%, be crushed to 60 ~ 80 mesh, beneficial to being added in feed, help disappearing for Bostrichthys sinensis
Change, improve grazing rate.
Compound Chinese herb is made up of following abundant and weight portion:12 ~ 18 parts of rhodiola root, 8 ~ 12 parts of the Radix Astragali, 8 ~ 12 parts of matrimony vine,
6 ~ 10 parts of Radix Isatidis, 3 ~ 5 parts of Radix Codonopsis, 6 ~ 10 parts of cordate houttuynia, 6 ~ 8 parts of Radix Angelicae Sinensis, 3 ~ 5 parts of Radix Glycyrrhizae, old 6 ~ 10 parts of bacterium, Semen Cuscutae
6 ~ 10 parts, 6 ~ 10 parts of thizoma curculiginis, 5 ~ 7 parts of honeysuckle, 3 ~ 5 parts of the fleece-flower root, 1 ~ 5 part of realgar, 0.002 ~ 0.005 part of unprocessed Radix Aconiti Lateralis.Can
Suppress the breeding and growth of harmful bacteria in Bostrichthys sinensis enteron aisle well, antibiotic medicine, noresidue, in not influenceing can be replaced
The growth of magnificent Bostrichthys sinensis and quality.
Fermenting mixture is made up of following component and weight portion in step 3:3 ~ 5 parts of soluble starch, 2 ~ 3 parts of beancake powder,
0.01 ~ 0.03 part of DPHP, 0.02 ~ 0.04 part of alizarin red, 0.3 ~ 0.5 part of potassium dihydrogen phosphate, MgSO4.7H2O
0.01 ~ 0.03 part, CaCl20.005 ~ 0.012 part, ferment effect is lifted, increase the content of beneficial bacterium in Chinese herbal and crude drugs preparations.
Beneficial effect:The compound Chinese herb that the present invention is used has suppression harmful bacteria, promotes digestion, improves Bostrichthys sinensis
Immunity and resisting stress power, extracting effect can be obviously improved using Ultrasonic Wave-Assisted Extraction, using multi-cultur es two-step fermentation and system
Standby fermenting mixture lifting ferment effect, shortens fermentation time, and Chinese herbal and crude drugs preparations prepared by the present invention can suppress Chinese Wu Tang
The harmful bacteria breeding and growth in Eclipta prostrata road, effect substantially, can replace antibiotic usage, noresidue, the growth to Bostrichthys sinensis
Without influence, be conducive to lifting culture benefit.
Specific embodiment
It is described in further detail with reference to embodiments:
Embodiment 1:
A kind of Chinese herbal and crude drugs preparations for improving Bostrichthys sinensis immunity, preparation process is as follows:
1)Pretreatment:Compound Chinese herb is crushed, is mixed, be made compound Chinese herb powder;
2)Extraction:The pure water for adding compound Chinese herb powder to accumulate 5 ~ 11 times, Ultrasonic Wave-Assisted Extraction;
3)Fermentation:Fermenting mixture is added after extraction, adjusts pH value to 7 ~ 7.2, be made zymotic fluid, zymotic fluid is added into fermentation tank
In, bacillus subtilis and S. cervisiae are inoculated with, aerobic fermentation is inoculated with lactobacillus bulgaricus and bifidobacterium bifidum, detests
Aerobe fermentation;
4)Post processing:Anaerobic fermented liquid is adsorbed with wheat bran, be dry, pulverize, sieved, obtain Chinese herbal and crude drugs preparations.
Compound Chinese herb is crushed to 40 ~ 70 mesh in step 1, extracting effect is lifted, by the beneficiating ingredient extraction in herbal medicine
Out.
In step 2 under ultrasonic assistant, 20 ~ 30min is extracted under the conditions of 90 ~ 110 DEG C, lift extracting effect, improve leaching
Soluble beneficiating ingredient content in extract.
The zymotic fluid of fermentation tankage size 60 ~ 71% is added in step 3, bacillus subtilis and S. cervisiae, inoculation is inoculated with
Amount is respectively 2 ~ 4%, 4.8 ~ 8%, and it is 27 ~ 37 DEG C to control fermentation temperature, 150 ~ 300rpm of rotating speed, 10 ~ 20L/min of throughput, hair
12 ~ 24h of ferment, after fermentation ends, is inoculated with lactobacillus bulgaricus and bifidobacterium bifidum, and inoculum concentration is 2 ~ 4%, and fermentation temperature is
28 ~ 37 DEG C, ferment 12 ~ 24h.The active polysaccharide in Chinese herbal and crude drugs preparations is improved, beneficial to the immunity and product that improve Bostrichthys sinensis
Matter.
Bacillus subtilis(Chinese agriculture Microbiological Culture Collection administrative center, bacterium numbering ACCC10619), wine brewing
Saccharomycete (Angel Yeast), lactobacillus bulgaricus(Chinese agriculture Microbiological Culture Collection administrative center, bacterium numbering
ACCC10638)And bifidobacterium bifidum(ATCC standard strains, bacterium numbering ATCC29521)
Wheat bran volume is 1 ~ 2 times of fermentating liquid volume in step 4, beneficiating ingredient in zymotic fluid can be preferably absorbed, at 40 ~ 60 DEG C
Under the conditions of dry, moisture control 8 ~ 10%, be crushed to 60 ~ 80 mesh, beneficial to being added in feed, help disappearing for Bostrichthys sinensis
Change, improve grazing rate.
Compound Chinese herb is made up of following abundant and weight portion:12 ~ 18 parts of rhodiola root, 8 ~ 12 parts of the Radix Astragali, 8 ~ 12 parts of matrimony vine,
6 ~ 10 parts of Radix Isatidis, 3 ~ 5 parts of Radix Codonopsis, 6 ~ 10 parts of cordate houttuynia, 6 ~ 8 parts of Radix Angelicae Sinensis, 3 ~ 5 parts of Radix Glycyrrhizae, old 6 ~ 10 parts of bacterium, Semen Cuscutae
6 ~ 10 parts, 6 ~ 10 parts of thizoma curculiginis, 5 ~ 7 parts of honeysuckle, 3 ~ 5 parts of the fleece-flower root, 1 ~ 5 part of realgar, 0.002 ~ 0.005 part of unprocessed Radix Aconiti Lateralis.Can
Suppress the breeding and growth of harmful bacteria in Bostrichthys sinensis enteron aisle well, antibiotic medicine, noresidue, in not influenceing can be replaced
The growth of magnificent Bostrichthys sinensis and quality.
Fermenting mixture is made up of following component and weight portion in step 3:3 ~ 5 parts of soluble starch, 2 ~ 3 parts of beancake powder,
0.01 ~ 0.03 part of DPHP, 0.02 ~ 0.04 part of alizarin red, 0.3 ~ 0.5 part of potassium dihydrogen phosphate, MgSO4.7H2O
0.01 ~ 0.03 part, CaCl20.005 ~ 0.012 part, DPHP can combine compound Chinese herb with potassium dihydrogen phosphate
In active ingredient, produce more beneficial bacterium, so as to lift ferment effect, increase the content of beneficial bacterium in Chinese herbal and crude drugs preparations.
Embodiment 2:
A kind of Chinese herbal and crude drugs preparations for improving Bostrichthys sinensis immunity, preparation process is as follows:
1)Pretreatment:Compound Chinese herb is crushed, is mixed, be made compound Chinese herb powder;
2)Extraction:The pure water for adding preferred compound Chinese herb powder to accumulate 8 times, Ultrasonic Wave-Assisted Extraction;
3)Fermentation:Fermenting mixture is added after extraction, adjusts pH value to preferred 7, be made zymotic fluid, zymotic fluid added and is fermented
In tank, bacillus subtilis and S. cervisiae are inoculated with, aerobic fermentation is inoculated with lactobacillus bulgaricus and bifidobacterium bifidum,
Anaerobic fermentation;
4)Post processing:Anaerobic fermented liquid is adsorbed with wheat bran, be dry, pulverize, sieved, obtain Chinese herbal and crude drugs preparations.
Compound Chinese herb is crushed to preferred 60 mesh in step 1, extracting effect is lifted, by the beneficiating ingredient leaching in herbal medicine
Put forward.
In step 2 under ultrasonic assistant, preferred 25min, lifting extraction effect are extracted under the conditions of preferred 100 DEG C
Really, the soluble beneficiating ingredient content in leaching liquor is improved.
The zymotic fluid of preferred fermentation tankage size 65% is added in step 3, bacillus subtilis and S. cervisiae is inoculated with,
Inoculum concentration is respectively preferred 3%, 5%, controls fermentation temperature for preferred 30 DEG C, preferably rotating speed 200rpm, preferably throughput
15L/min, preferably ferment 16h, after fermentation ends, is inoculated with lactobacillus bulgaricus and bifidobacterium bifidum, and inoculum concentration is preferred
3%, fermentation temperature is preferred 33 DEG C, and ferment preferred 15h.The active polysaccharide in Chinese herbal and crude drugs preparations is improved, it is Chinese beneficial to improving
The immunity and quality of Bostrichthys sinensis.
Bacillus subtilis(Chinese agriculture Microbiological Culture Collection administrative center, bacterium numbering ACCC10619), wine brewing
Saccharomycete (Angel Yeast), lactobacillus bulgaricus(Chinese agriculture Microbiological Culture Collection administrative center, bacterium numbering
ACCC10638)And bifidobacterium bifidum(ATCC standard strains, bacterium numbering ATCC29521)
Wheat bran volume described in step 4 is preferred 2 times of fermentating liquid volume, can preferably absorb beneficiating ingredient in zymotic fluid,
Dried under the conditions of preferred 50 DEG C, moisture is controlled 8 ~ 10%, is crushed to preferred 75 mesh, beneficial to being added in feed, in helping
The digestion of magnificent Bostrichthys sinensis, improves grazing rate.
Compound Chinese herb is made up of following abundant and preferred weight part:16 parts of rhodiola root, 11 parts of the Radix Astragali, 10 parts of matrimony vine, plate
7 parts of blue root, 4 parts of Radix Codonopsis, 8 parts of cordate houttuynia, 7 parts of Radix Angelicae Sinensis, 4 parts of Radix Glycyrrhizae, old 8 parts of bacterium, 8 parts of Semen Cuscutae, 9 parts of thizoma curculiginis, honeysuckle
6 parts, 4 parts of the fleece-flower root, 3 parts of realgar, 0.004 part of unprocessed Radix Aconiti Lateralis.The breeding of harmful bacteria in Bostrichthys sinensis enteron aisle can well be suppressed
And growth, antibiotic medicine can be replaced, noresidue does not influence growth and the quality of Bostrichthys sinensis.
Fermenting mixture is made up of following component and preferred weight part in step 3:4 parts of soluble starch, beancake powder 2.6
Part, 0.02 part of DPHP, 0.03 part of alizarin red, 0.4 part of potassium dihydrogen phosphate, MgSO4.7H20.02 part of O, CaCl2
0.011 part, ferment effect is lifted, increase the content of beneficial bacterium in Chinese herbal and crude drugs preparations.
Embodiment of above is merely to illustrate the present invention, and not limitation of the present invention, the ordinary skill people of this area
Member, without departing from the spirit and scope of the present invention, can also make a variety of changes and modification.Therefore, it is all equivalent
Technical scheme fall within scope of the invention, scope of patent protection of the invention should be defined by the claims.
Claims (7)
1. it is a kind of improve Bostrichthys sinensis immunity Chinese herbal and crude drugs preparations, it is characterised in that:Preparation process is as follows:
1)Pretreatment:Compound Chinese herb is crushed, is mixed, be made compound Chinese herb powder;
2)Extraction:The pure water for adding compound Chinese herb powder to accumulate 5 ~ 11 times, Ultrasonic Wave-Assisted Extraction;
3)Fermentation:Fermenting mixture is added after extraction, pH value is adjusted, zymotic fluid is made, by zymotic fluid addition fermentation tank, is inoculated with
Bacillus subtilis and S. cervisiae, aerobic fermentation are inoculated with lactobacillus bulgaricus and bifidobacterium bifidum, anaerobic fermentation;
4)Post processing:Anaerobic fermented liquid is adsorbed with wheat bran, be dry, pulverize, sieved, obtain Chinese herbal and crude drugs preparations.
2. it is according to claim 1 it is a kind of improve Bostrichthys sinensis immunity Chinese herbal and crude drugs preparations, it is characterised in that:It is described
Compound Chinese herb is crushed to 40 ~ 70 mesh in step 1.
3. it is according to claim 1 it is a kind of improve Bostrichthys sinensis immunity Chinese herbal and crude drugs preparations, it is characterised in that:It is described
In step 2 under ultrasonic assistant, 20 ~ 30min is extracted under the conditions of 90 ~ 110 DEG C.
4. it is according to claim 1 it is a kind of improve Bostrichthys sinensis immunity Chinese herbal and crude drugs preparations, it is characterised in that:It is described
The zymotic fluid of fermentation tankage size 60 ~ 71% is added in step 3, bacillus subtilis and S. cervisiae is inoculated with, inoculum concentration is respectively
2 ~ 4%, 4.8 ~ 8%, it is 27 ~ 37 DEG C to control fermentation temperature, 150 ~ 300rpm of rotating speed, 10 ~ 20L/min of throughput, and ferment 12 ~ 24h,
After fermentation ends, lactobacillus bulgaricus and bifidobacterium bifidum are inoculated with, inoculum concentration is 2 ~ 4%, and fermentation temperature is 28 ~ 37 DEG C, hair
12 ~ 24h of ferment.
5. it is according to claim 1 it is a kind of improve Bostrichthys sinensis immunity Chinese herbal and crude drugs preparations, it is characterised in that:It is described
Wheat bran volume described in step 4 is 1 ~ 2 times of fermentating liquid volume, is dried under the conditions of 40 ~ 60 DEG C, and moisture is controlled in 8 ~ 10%, powder
It is broken to 60 ~ 80 mesh.
6. it is according to claim 1 it is a kind of improve Bostrichthys sinensis immunity Chinese herbal and crude drugs preparations, it is characterised in that:It is described
Compound Chinese herb is made up of following abundant and weight portion:12 ~ 18 parts of rhodiola root, 8 ~ 12 parts of the Radix Astragali, 8 ~ 12 parts of matrimony vine, Radix Isatidis 6 ~
10 parts, 3 ~ 5 parts of Radix Codonopsis, 6 ~ 10 parts of cordate houttuynia, 6 ~ 8 parts of Radix Angelicae Sinensis, 3 ~ 5 parts of Radix Glycyrrhizae, old 6 ~ 10 parts of bacterium, 6 ~ 10 parts of Semen Cuscutae,
6 ~ 10 parts of thizoma curculiginis, 5 ~ 7 parts of honeysuckle, 3 ~ 5 parts of the fleece-flower root, 1 ~ 5 part of realgar, 0.002 ~ 0.005 part of unprocessed Radix Aconiti Lateralis.
7. it is according to claim 1 it is a kind of improve Bostrichthys sinensis immunity Chinese herbal and crude drugs preparations, it is characterised in that:It is described
Fermenting mixture is made up of following component and weight portion in step 3:3 ~ 5 parts of soluble starch, 2 ~ 3 parts of beancake powder, two palmityls
0.01 ~ 0.03 part of hydroxyproline, 0.02 ~ 0.04 part of alizarin red, 0.3 ~ 0.5 part of potassium dihydrogen phosphate, MgSO4.7H2O 0.01~0.03
Part, CaCl20.005 ~ 0.012 part.
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Cited By (1)
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CN107751567A (en) * | 2017-10-13 | 2018-03-06 | 无锡正大生物股份有限公司 | The preparation method of feed slow release compound stevioside micro-capsule |
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CN105661013A (en) * | 2016-03-09 | 2016-06-15 | 广东海洋大学 | Method for preparing compound type growth-promoting feed additive by fermenting Chinese herbal medicines |
CN105876129A (en) * | 2016-03-08 | 2016-08-24 | 广州三行生物科技有限公司 | Fermented Chinese herbal medicine preparation replacing antibiotics and preparation method and application thereof |
CN106071405A (en) * | 2016-06-27 | 2016-11-09 | 青岛铭晨信息技术有限公司 | A kind of preparation method and application of Penaeus vannamei Chinese medicine immunity enhancer |
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CN103598485A (en) * | 2013-12-06 | 2014-02-26 | 青岛嘉瑞生物技术有限公司 | Preparation method and application of Chinese herbal immunopotentiator used for snakeheads |
CN105876129A (en) * | 2016-03-08 | 2016-08-24 | 广州三行生物科技有限公司 | Fermented Chinese herbal medicine preparation replacing antibiotics and preparation method and application thereof |
CN105661013A (en) * | 2016-03-09 | 2016-06-15 | 广东海洋大学 | Method for preparing compound type growth-promoting feed additive by fermenting Chinese herbal medicines |
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