CN106720683A - A kind of method for preparing functional black tea bacteria beverage as raw material with matrimony vine honeysuckle black tea - Google Patents
A kind of method for preparing functional black tea bacteria beverage as raw material with matrimony vine honeysuckle black tea Download PDFInfo
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- CN106720683A CN106720683A CN201611181459.1A CN201611181459A CN106720683A CN 106720683 A CN106720683 A CN 106720683A CN 201611181459 A CN201611181459 A CN 201611181459A CN 106720683 A CN106720683 A CN 106720683A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/163—Liquid or semi-liquid tea extract preparations, e.g. gels, liquid extracts in solid capsules
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/166—Addition of, or treatment with, enzymes or microorganisms
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/82—Theaceae (Tea family), e.g. camellia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
Abstract
The present invention relates to a kind of method for preparing functional black tea bacteria beverage as raw material with matrimony vine honeysuckle black tea, with matrimony vine, honeysuckle, black tea and white granulated sugar as raw material, fermented tea is strain to methods described, by way of shaker fermentation, obtains functional black tea bacteria beverage.This method adds matrimony vine and honeysuckle in Tea Fungus Fermentation, on the basis of the original sour-sweet aromatic quality of fermented tea is retained, increased the nutritional ingredient in red tea fungus fermented date liquid, traditional standing for fermentation instead of using oscillatory ventilation fermentation method, oscillatory ventilation culture is more conducive to the maturation of fermented tea, shortens the fermentation period of black-tea fungus drink, through zoopery evaluation, obtained beverage vessel is significantly increased the effect of immunity of organisms, is a kind of rising health care drink.
Description
Technical field
It is especially a kind of red as raw material preparation feature with matrimony vine honeysuckle black tea the invention belongs to functional beverage field
The method of tea bacteria beverage.
Background technology
Used as a kind of acidic beverages of folk tradition, it is to be with glucose-tea broth by acetic acid bacteria, saccharomycete, lactic acid bacteria to fermented tea
The fermented beverage that raw material is made.Contain the various compounds and trace element beneficial to human body in red tea fungus fermented date liquid, to various
The effect of chronic disease such as hypertension, high fat of blood, artery sclerosis, diabetes etc. with treatment with prevention, is a kind of pure natural
Microbial fermentation product.At present, fermented tea fermented beverage kind is more single, and concrete function is indefinite, which has limited fermented tea
The development of beverage, so the research to functional black tea bacteria beverage has good economic worth.
Matrimony vine contains various nutrition and active component as the plant of medicine-food two-purpose.Modern study shows that matrimony vine mainly contains
LBP-X, glycine betaine, atropine, hyoscyamine, with promote immune, anti-aging, it is antitumor, remove free radical, it is antifatigue,
The effects such as radioresistance, liver protection, protection and improvement reproductive function.Honeysuckle is conventional traditional Chinese medicine, with anti-inflammatory, antibacterial, is resisted
Various pharmacological activities such as viral, anti-oxidant, are widely used in food, medicine and health products.
By retrieval, the following several patent publication us related to the present patent application patent are found：
1st, a kind of preparation method of black-tea fungus drink (CN102440424A), specifically includes：With matrimony vine as primary raw material system
Standby fructus lycii extracted solution, is bacterium source with fermented tea then again with fructus lycii extracted solution as culture medium, with brown sugar as carbon source, is prepared new
The medlar black tea bacteria beverage of type characteristic.By implementing the present invention, matrimony vine and fermented tea are combined, it is combined
Nutriment more enriches；Matrimony vine and fermented tea all have significant health-care efficacy to human body diseases, after the two unites two into one, its
Effect complements each other again, and tea beverage its health-care efficacy for being formed more shows.Far super simple matrimony vine is made its resultant effect
Various food and traditional black-tea fungus drink.Meanwhile, the medlar black tea bacteria beverage for preparing remains matrimony vine to greatest extent
The multiple biological activities beneficial to human body are into sweet mouthfeel is agreeable to the taste, and palatability is very good, and applicable crowd is extremely wide, is a kind of
Have both beverage and have the dual-use function ecological health drink of medical efficacy, market prospects are very good.
2nd, a kind of preparation method of black-tea fungus drink (CN102440424A), is related to a kind of preparation side of black-tea fungus drink
Method, first by date, the fruit of Chinese wolfberry, fresh ginger etc. with clear water clean after in the ratio of raw material and water boil filtering, remove slag lower the temperature into
In Plastic Drum, add black tea strain ferment one week be finished product Chang Yinhou can benefiting qi and nourishing blood, in vigorous health, to constitution compared with weak person spy
Strengthen immunity is not conducive to suitably.
By contrast, there is the difference of essence with above-mentioned patent publication us in present patent application.
The content of the invention
It is an object of the invention to overcome the deficiencies in the prior art part, there is provided one kind is with matrimony vine honeysuckle black tea as raw material
The method for preparing functional black tea bacteria beverage, the method adds matrimony vine and honeysuckle in Tea Fungus Fermentation, is retaining
On the basis of the original sour-sweet aromatic quality of fermented tea, the nutritional ingredient in red tea fungus fermented date liquid is increased, oscillatory ventilation culture is more
Beneficial to the maturation of fermented tea, through zoopery evaluation, obtained beverage vessel is significantly increased the effect of immunity of organisms, is a kind of
Rising health care drink.
The present invention solves its technical problem and takes following technical scheme to realize：
It is a kind of with matrimony vine honeysuckle black tea as raw material prepare functional black tea bacteria beverage method, methods described with matrimony vine,
Honeysuckle, black tea and white granulated sugar are raw material, and fermented tea is strain, by way of shaker fermentation, obtain functional black tea bacterium drink
Material.
And, comprise the following steps that：
(1) the preparation of black tea mother bacterial liquid：Black tea and white granulated sugar are added in water, sterilize 15min after boiling at 121 DEG C, cool
To room temperature, glucose-tea broth is obtained, to fermented tea strain liquid of the OD values for 0.835 is added in glucose-tea broth, sealed with eight layers of gauze, shaking table
Fermentation 4-5 days, that is, obtain fermented tea mother liquor；
Wherein, the black tea and the mass ratio of water are 0.004~0.01:1, the mass ratio of white granulated sugar and water for 0.04~
0.07:1, the fermented tea strain liquid is 0.02 with the volume ratio of glucose-tea broth:1~0.005:1；
(2) the preparation of leaching liquor：Ready black tea, matrimony vine and honeysuckle are put into water and boil 80~120min, afterwards
Residue is filtered off, white granulated sugar is added in filter liquor, then filtered, the solution after filtering sterilizes 15min at 121 DEG C, taken out simultaneously
It is cool to room temperature, obtain final product leaching liquor；
Wherein, the black tea and the mass ratio of water are 0.004~0.01:1, the mass ratio of matrimony vine and water for 0.005~
0.02:1, honeysuckle is 0.002~0.008 with the mass ratio of water:1, white granulated sugar is 0.04~0.07 with the mass ratio of water:1；
(3) it is inoculated with：The volume of black tea mother bacterial liquid, the black tea mother bacterial liquid and leaching liquor is added in leaching liquor after sterilization
Than being 0.02:1~0.005:1；
(4) ferment：The liquid that will be inoculated with is positioned in 27~37 DEG C of shaking tables, is 27~37 DEG C in fermentation temperature, and shaking table turns
To be fermented under conditions of 80~120r/min, fermentation time is 4~5 days to speed, obtains final product functional black tea bacteria beverage.
And, (1) middle boiling time is 80~120min to the step.
And, the step is (2) middle to seal filtering using eight layers of gauze.
The advantage for obtaining of the invention and good effect are：
1st, this method adds matrimony vine and honeysuckle in Tea Fungus Fermentation, and retaining, fermented tea is original sour-sweet aromatic
On the basis of quality, the nutritional ingredient in red tea fungus fermented date liquid is increased, be instead of using oscillatory ventilation fermentation method traditional
Standing for fermentation, oscillatory ventilation culture is more conducive to the maturation of fermented tea, shortens the fermentation period of black-tea fungus drink, is commented through zoopery
Valency, obtained beverage vessel is significantly increased the effect of immunity of organisms, is a kind of rising health care drink.
2nd, the composition in black-tea fungus drink obtained in this method has carried out analysis measure, as a result finds, sugar contains in fermented tea
It is 26.89g/L to measure, and protein content is 1.67mg/mL, and total acid content is 18.35g/L, and acetic acid content is 15.68g/L, lactic acid
Content is 1.7mg/L, and glucuronic acid content is 0.096mg/L, and content of caffeine is 11.95mg/L, and polyphenol content is
0.23mg/mL, chlorogenic acid content is 23.9mg/L, and Vitamin C content is 17.26mg/L, and free aminoacid content is
77.67mg/L, the content of D-Glucose diacid-Isosorbide-5-Nitrae-lactone is 8.54mg/mL.
3rd, this method instead of traditional standing for fermentation using oscillatory ventilation fermentation method, and oscillatory ventilation fermentation is more beneficial for
The maturation of fermented tea, shortens the fermentation period of black-tea fungus drink.
4th, this method sterilizes when black-tea fungus drink is prepared to leaching liquor, it is to avoid the pollution of other miscellaneous bacterias, it is ensured that
The security of product.
5th, this method adds matrimony vine and honeysuckle in Tea Fungus Fermentation, and retaining, fermented tea is original sour-sweet aromatic
On the basis of quality, the nutritional ingredient in red tea fungus fermented date liquid is increased, often drinking can improve autoimmunity.
Brief description of the drawings
Fig. 1 is influence figure of the matrimony vine honeysuckle black-tea fungus drink to hypoimmunity mice organ index in the present invention；
Fig. 2 is influence figure of the matrimony vine honeysuckle black-tea fungus drink to hypoimmunity mice leukocyte count in the present invention；
Fig. 3 for the present invention in matrimony vine honeysuckle black-tea fungus drink to hypoimmunity mice peritoneal macrophage swallow dimethyl diaminophenazine chloride
The influence figure of phagocytic activity；
Fig. 4 for the present invention in matrimony vine honeysuckle black-tea fungus drink NK in hypoimmunity mice spleen is killed
The influence figure of activity；
Fig. 5 for the present invention in matrimony vine honeysuckle black-tea fungus drink to hypoimmunity mice spleen medium size lymphocyte multiplication capacity
Influence figure.
Specific embodiment
With reference to embodiment, the present invention is further described；Following embodiments are illustrative, be not it is limited,
Protection scope of the present invention can not be limited with following embodiments.
Raw material used in the present invention, unless otherwise specified, is conventional commercially available prod；Used in the present invention
Method, unless otherwise specified, is the conventional method of this area.
Embodiment 1：
It is a kind of with matrimony vine honeysuckle black tea as raw material prepare functional black tea bacteria beverage method, methods described with matrimony vine,
Honeysuckle, black tea and white granulated sugar are raw material, and fermented tea is strain, by way of shaker fermentation, obtain functional black tea bacterium drink
Material；
Comprise the following steps that：
(1) the preparation of black tea mother bacterial liquid：Black tea and white granulated sugar are added in water, sterilize 15min after boiling at 121 DEG C, cool
To room temperature, glucose-tea broth is obtained, to fermented tea strain liquid of the OD values for 0.835 is added in glucose-tea broth, sealed with eight layers of gauze, shaking table
Fermentation 4-5 days, that is, obtain fermented tea mother liquor；
Wherein, the black tea and the mass ratio of water are 0.004~0.01:1, the mass ratio of white granulated sugar and water for 0.04~
0.07:1, the fermented tea strain liquid is 0.02 with the volume ratio of glucose-tea broth:1~0.005:1；
(2) the preparation of leaching liquor：4g black tea, 5g matrimony vines and 2g honeysuckles are added in 1L water, are boiled and is kept
90min, filters off residue afterwards, and 40g white granulated sugars are added in filter liquor, is sealed with eight layers of gauze, and is sterilized at 121 DEG C
15min, takes out and cool to room temperature, obtains final product leaching liquor；
(3) it is inoculated with：The body of 8mL black tea mother bacterial liquids, the black tea mother bacterial liquid and leaching liquor is added in leaching liquor after sterilization
Product is than being 0.02:1~0.005:1；
(4) ferment：The liquid that will be inoculated with is positioned in shaking table, is 32 DEG C in fermentation temperature, and shaking speed is 90r/min
Under conditions of fermented, fermentation time be 5 days, obtain final product functional black tea bacteria beverage.
Embodiment 2：
It is a kind of with matrimony vine honeysuckle black tea as raw material prepare functional black tea bacteria beverage method, methods described with matrimony vine,
Honeysuckle, black tea and white granulated sugar are raw material, and fermented tea is strain, by way of shaker fermentation, obtain functional black tea bacterium drink
Material；
Comprise the following steps that：
(1) the preparation of black tea mother bacterial liquid：Black tea and white granulated sugar are added in water, sterilize 15min after boiling at 121 DEG C, cool
To room temperature, glucose-tea broth is obtained, to fermented tea strain liquid of the OD values for 0.835 is added in glucose-tea broth, sealed with eight layers of gauze, shaking table
Fermentation 4-5 days, that is, obtain fermented tea mother liquor；
Wherein, the black tea and the mass ratio of water are 0.004~0.01:1, the mass ratio of white granulated sugar and water for 0.04~
0.07:1, the fermented tea strain liquid is 0.02 with the volume ratio of glucose-tea broth:1~0.005:1；
(2) the preparation of leaching liquor：10g black tea, 20g matrimony vines and 8g honeysuckles are added in 1L water, are boiled and is kept
120min, filters off residue afterwards, and 70g white granulated sugars are added in filter liquor, is sealed with eight layers of gauze, and is sterilized at 121 DEG C
15min, takes out and cool to room temperature, obtains final product leaching liquor；
(3) it is inoculated with：11mL black tea mother bacterial liquids, the black tea mother bacterial liquid and leaching liquor are added in leaching liquor after sterilization
Volume ratio is 0.02:1~0.005:1；
(4) ferment：The liquid that will be inoculated with is positioned in shaking table, is 35 DEG C in fermentation temperature, and shaking speed is 120r/min
Under conditions of fermented, fermentation time be 4 days, obtain final product functional black tea bacteria beverage.
The related test results of the functional black tea bacteria beverage that the inventive method is prepared：
1st, immunoregulation effect of the matrimony vine honeysuckle fermented tea fermented beverage to BALB/c mouse
1.1 zooperies
Male BALB/c mouse 72, body weight 18-22g, purchase and blood disease hospital of the Chinese Academy of Medical Sciences, licensing
Number：SCXK (Tianjin) 2009-0002.All mouse are fed in (the environmental facility conjunction of University Of Science and Technology Of Tianjin's cleaning grade zoopery room
Lattice card number：SYXK (Tianjin) 2006-0005), environment temperature is that (22 ± 2) DEG C, humidity are 40%-60%, is handed in illumination in 12 hours
Male and female are separately raised in the environment for replacing, and freely take the photograph water, feeding, and experiment is started after adapting to one week.
The intervention of 1.2 animal packets and tested material
Mouse is randomly divided into six groups, i.e.,：Normal group (N), model control group (C), positive controls (P), fermented tea
Beverage low (L), in (M), high dose group (H), every group 12；In addition to Normal group, the intraperitoneal injection for three days on end of each group mouse
The endoxan of 80mg/kg sets up immune deficiency model, and Normal group is replaced with physiological saline；From first day of modeling,
Each experimental mice distinguishes black-tea fungus drink (the L groups of gavage respective concentration：2mg/mL；M groups：4mg/mL；H groups：8mg/mL), sun
Property control be commercially available Huang Qi Jing, Normal group and model control group replace with physiological saline, once a day, continuous 13 days.It is small
Mouse injection volume and gavage amount are 0.05mL/10g, every 3 days weight of being weighed in the set time once and recorded.
Influence of the 1.3 matrimony vine honeysuckle black-tea fungus drinks of the present invention to hypoimmunity mice body weight
The changes of weight (Mean ± SD, n=12) of each group mouse of table 1
Note：* compare with model control group, P<0.05, * * compares with model control group, P<0.01；# and Normal group ratio
Compared with P<0.05, ## compares with Normal group, P<0.01
As seen from Table 1, before and after testing the body weight ascendant trend of Normal group mouse substantially, illustrate under normal circumstances with
Experimental period increase, Mouse Weight should constantly rise；By contrast, model group C groups Mouse Weight is presented obvious decline
Trend, for three days on end after injection endoxan, mouse body function sustains damage, and causes mouse in feeding process for this explanation
Food-intake and amount of drinking water are reduced, and in the presence of composite factor, ultimately result in mouse phase body weight degradation after the test；It is positive
Control group, black-tea fungus drink low dose group, black-tea fungus drink middle dose group, the body weight of black-tea fungus drink high dose group mouse first under
Rise after drop, illustrate that endoxan can be injected to mouse with the increase astragalus membranaceous and black-tea fungus drink of gavage time
The Body weight loss symptom for producing afterwards has certain restitution, can be from the immune function of side reflection black-tea fungus drink regulation.
Influence of the 1.4 matrimony vine honeysuckle black-tea fungus drinks of the present invention to hypoimmunity mice organ index
The weight of pluck organ, form, color and luster and organ index are the important indicators for weighing animal health condition.According to
Body disease degree can be substantially determined according to organ weights, organ coefficient, so organ index is the basis commonly used in zoopery
Data.
Immune organ is immunocyte development, ripe, colonization, propagation and the place for producing immune response[68].Thymus gland and
Spleen is the Main Immune Organs of body, with specific immunity have it is close contact, the organ index of the two can reflect it
Development and function status, so as to reflect the immune level of body indirectly.
From figure 1 it appears that mouse is after continuous injection endoxan, internal immune organ has in various degree
Damage, thymus gland increase, cause thymus index to increase；Spleen reduces, and causes index and spleen index to reduce；This is probably mouse injection ring
After phosphamide, it is suppressed as the spleen of immune organ, causes thymus gland compensatory to increase, so that thymus index increases.
Compared with Normal group, the thymus index conspicuousness of model group mouse declines, and index and spleen index conspicuousness rises, and illustrates ring phosphinylidyne
Immunodeficiency models modeling success caused by amine；Compared with model group, positive controls mouse thymus index slightly has rising, spleen
Index is declined slightly, but without conspicuousness；By contrast, black-tea fungus drink dosage group is to hypoimmunity mice thymus index and spleen
Index returns to normal level and plays a role.
Influence of the 1.5 matrimony vine honeysuckle black-tea fungus drinks of the present invention to hypoimmunity mice leukocyte count
Leucocyte is divided into granulocyte, monocyte and the class of lymphocyte three, is widely present in blood, lymph and tissue,
It is a kind of important haemocyte in peripheral blood.Leucocyte resists the important component of system as body, with active shifting
Kinetic force, can do amoeboid movement, with defence and immunologic function, can resist by different mechanism of action and eliminate invasion machine
The pathogen of body.
From figure 2 it can be seen that the leukocyte count of model group mouse is presented extremely significant decline compared with Normal group
Trend (P<0.01) the immunodeficiency models modeling success of caused by cyclophosphamide, is illustrated；Positive controls can be significantly improved to be exempted from
Leukocyte count (the P of the low mouse of epidemic disease<0.05), and black-tea fungus drink dosage group has to the reduction of the leukocyte count of hypoimmunity mice
Good restitution, and as the increase of dosage is in rising trend, the significant difference compared with model group.Illustrate that fermented tea is drunk
Expect there is good restitution to the decline of the hypoimmunity mice leukocyte count of caused by cyclophosphamide.
1.6 matrimony vine honeysuckle black-tea fungus drinks of the present invention are to the phagocytosis dimethyl diaminophenazine chloride phagocytosis of hypoimmunity mice peritoneal macrophage
The influence of ability
Macrophage plays a significant role in body nospecific immunity, widely distributed in body.Macrophage is made
It is the cell that biological action in body is the most active, the antigen of body can be entered from any position in very first time identification,
Congenital immunity effect is played, antigen invasion is resisted.
As seen from Figure 3, the phagocytic activity pole conspicuousness ground of model control group Turnover of Mouse Peritoneal Macrophages is less than normal
Control group (P<0.01), positive controls pair and the phagocytic activity of other experimental mices then has difference compared with model control group
The enhancing of degree.Influence of the black-tea fungus drink dosage group to peritoneal macrophage phagocytic activity is in dose dependent, wherein black tea
Bacteria beverage high dose group effect conspicuousness increases (P<0.01).Test result indicate that black-tea fungus drink can promote endoxan to cause
The phagocytic activity of hypoimmunity mice peritoneal macrophage plays its immunoregulation effect.
1.7 matrimony vine honeysuckle black-tea fungus drinks of the present invention are to Cytotoxicity of Natural Killer Cells in hypoimmunity mice spleen
Influence
NK cells are primarily present in spleen, are one of important nospecific immunity cells of body.It is natural as body
Immunocyte, with nature cell cytotoxic activity, participates in the functions such as antitumor, anti-infective, immunological regulation, in immunity of organism monitoring
Play an important role, be the first line of defence that body resists exotic antigen or " non-self material ", and the NK cells of activation can also
Secretion cytokine profiles participate in immunological regulation so as to strengthen immunosurveillance.Therefore, NK cytoactives energy direct reaction body is exempted from
The functional level of epidemic disease system.
As shown in Figure 4, the NK cell killing activities pole conspicuousness of model control group mouse is less than Normal group mouse (P<
0.01), fermented tea dosage group has certain restitution to the killing activity of the NK cells of endoxan cause hypoimmunity mice
But effect is not notable, positive controls are suitable with fermented tea middle dose group to the recovery capability of NK cell killing activities.This explanation,
Reduction of the fermented tea to the NK cell killing activities of endoxan cause hypoimmunity mice has certain adjustment effect, but effect is not
Significantly.
Influence of 1.8 black-tea fungus drinks of the present invention to hypoimmunity mice spleen medium size lymphocyte multiplication capacity
Lymphocyte proliferation assay, also known as lymphocyte transformation test, refers to the lymphocyte cultivated in vitro, by spy
After Specific Antigen or the former stimulation of non-specific mitogenesis, the lymphoblast that can enter line splitting is converted into, this process claims
It is lymphopoiesis or lymphoblastic transformation, the propagation of lymphocyte and differentiation are one during immune response important
In the stage, determine T lymphocytes and the multiplication capacity of bone-marrow-derived lymphocyte or the height of conversion ratio can be respectively as the thin of measurement body
Immune and humoral immunity level the important biochemical indicator of born of the same parents.
From figure 5 it can be seen that influence of the black-tea fungus drink to hypoimmunity mice T and B lymphocyte proliferation ability has
Identical trend.Compared with Normal group, model group mouse after continuous injection endoxan, T lymphocytes and B lymphs
The multiplication capacity of cell has substantially reduces (P<0.01)；Compared with model group, positive controls T and B lymphocyte proliferation energy
Power has pole conspicuousness increase (P<0.01), black-tea fungus drink dosage group is recovered also present to the multiplication capacity of T and bone-marrow-derived lymphocyte
Good trend, and have a certain amount-effect relation.Test result indicate that, black-tea fungus drink can significantly improve lymphocyte
Multiplication capacity, i.e. black-tea fungus drink can be strengthened in body by the thin of T cell mediateds by stimulating lymphocyte transformation
The function of born of the same parents' humoral immunity that is immune and being mediated by bone-marrow-derived lymphocyte.
Influence of 1.9 black-tea fungus drinks to enzyme activity in hypoimmunity mice liver and serum
1.9.1 influence of the black-tea fungus drink to enzyme activity in hypoimmunity mice liver
The black-tea fungus drink of table 2 causes the influence of hypoimmunity mice liver GSH-PX, AKP, SOD and CAT enzymatic activity to CY
(Mean ± SD, n=12)
Note：* compare with model control group, P<0.05, * * compares with model control group, P<0.01；# and Normal group ratio
Compared with P<0.05, ## compares with Normal group, P<0.01
Black-tea fungus drink causes the influence of hypoimmunity mice liver GSH-PX, AKP, SOD and CAT enzymatic activity to be shown in Table 2 CY,
Compared with Normal group, GSH-PX, AKP, SOD and CAT enzymatic activity have different degrees of reduction in model group mouse liver,
Wherein SOD enzyme activity conspicuousness reduction (P<0.05), AKP enzymatic activitys pole conspicuousness reduction (P<0.01), illustrate that endoxan presses down
The activity of mouse relevant enzyme is made.
Compared with model group (C groups) GSH-PX enzyme activities, Huang Qi Jing positive controls P groups, black-tea fungus drink are low, middle dosage
Group (L groups and M groups) GSH-PX enzyme activities have risen, but without significant difference (P>0.05)；Black-tea fungus drink high dose group
(H groups) is presented significant difference (P<0.05).
Compared with model group (C groups) AKP enzyme activities, Huang Qi Jing positive controls P groups, black-tea fungus drink are low, middle dose group
(L groups and M groups) AKP enzyme activities have risen, but without significant difference (P>0.05)；Black-tea fungus drink high dose group (H groups)
Significant difference (P is presented<, and effect is better than Normal group 0.01).
Compared with model group (C groups) SOD enzyme activities, Huang Qi Jing positive controls P groups, low, medium and high dose of black-tea fungus drink
Amount group (L groups, M groups and H groups) SOD enzyme activities have risen, but without significant difference (P>0.05).
Compared with model group (C groups) CAT enzyme activities, Huang Qi Jing positive controls P groups, low, medium and high dose of black-tea fungus drink
Amount group (L groups, M groups and H groups) CAT enzyme activities have risen, but without significant difference (P>0.05).
1.9.2 influence of the black-tea fungus drink to enzyme activity in hypoimmunity mice serum
The black-tea fungus drink of table 3 causes the influence of hypoimmunity mice serum GSH-PX, AKP, SOD and CAT enzymatic activity to CY
(Mean ± SD, n=12)
Note：* compare with model control group, P<0.05, * * compares with model control group, P<0.01；# and Normal group ratio
Compared with P<0.05, ## compares with Normal group, P<0.01
Black-tea fungus drink causes the influence of hypoimmunity mice serum GSH-PX, AKP, SOD and CAT enzymatic activity to be shown in Table 3 CY,
Compared with Normal group, GSH-PX, AKP, SOD and CAT enzymatic activity have different degrees of reduction in model group mice serum,
Wherein GSH-PX, AKP, SOD enzyme activity pole conspicuousness reduction (P<0.01), illustrate that endoxan inhibits the work of mouse relevant enzyme
Property.
Compared with model group (C groups) GSH-PX enzyme activities, Huang Qi Jing positive controls P groups, black-tea fungus drink are low, medium and high
Dosage group (L groups, M groups and H groups) GSH-PX enzyme activities have risen, but without significant difference (P>0.05).
Compared with model group (C groups) AKP enzyme activities, Huang Qi Jing positive controls P groups have pole significant difference (P<0.01),
Black-tea fungus drink middle dose group (M groups) AKP enzyme activities are presented significant difference (P<0.05)；Black-tea fungus drink high dose (H groups)
Group pole significant difference (P is being presented<0.01).
Compared with model group (C groups) SOD enzyme activities, Huang Qi Jing positive controls P groups, black-tea fungus drink are low, middle dose group
(L groups and M groups) SOD enzyme activities have risen, but without significant difference (P>0.05), black-tea fungus drink high dose group presents aobvious
Write sex differernce (P<0.05).
Compared with model group (C groups) CAT enzyme activities, Huang Qi Jing positive controls P groups, low, medium and high dose of black-tea fungus drink
Amount group (L groups, M groups and H groups) CAT enzyme activities have risen, but without significant difference (P>0.05).
2nd, the measure of matrimony vine honeysuckle fermented tea fermented beverage Mineral Elements in Jadeite Shellfish content
The present invention is determined by having carried out analysis to the composition in matrimony vine honeysuckle fermented tea fermented beverage, is as a result found,
Sugared content is 26.89g/L in fermented tea, and protein content is 1.67mg/mL, and total acid content is 18.35g/L, and acetic acid content is
15.68g/L, lactic acid content is 1.7mg/L, and glucuronic acid content is 0.096mg/L, and content of caffeine is 11.95mg/mL,
Polyphenol content is 0.23mg/mL, and chlorogenic acid content is 23.9mg/L, and Vitamin C content is 17.26mg/L, free amino acid
Content is 77.67mg/mL, and the content of D-Glucose diacid-Isosorbide-5-Nitrae-lactone is 8.54mg/mL.
Claims (4)
1. it is a kind of with matrimony vine honeysuckle black tea as raw material prepare functional black tea bacteria beverage method, it is characterised in that：The side
With matrimony vine, honeysuckle, black tea and white granulated sugar as raw material, fermented tea is strain to method, by way of shaker fermentation, obtains feature
Black-tea fungus drink.
2. it is according to claim 1 with matrimony vine honeysuckle black tea as raw material prepare functional black tea bacteria beverage method, its
It is characterised by：Comprise the following steps that：
(1) the preparation of black tea mother bacterial liquid：Black tea and white granulated sugar are added in water, sterilize 15min after boiling at 121 DEG C, cool to room
Wen Hou, obtains glucose-tea broth, to fermented tea strain liquid of the OD values for 0.835 is added in glucose-tea broth, is sealed with eight layers of gauze, shaker fermentation
4-5 days, that is, obtain fermented tea mother liquor；
Wherein, the black tea and the mass ratio of water are 0.004~0.01:1, white granulated sugar is 0.04~0.07 with the mass ratio of water:
1, the fermented tea strain liquid is 0.02 with the volume ratio of glucose-tea broth:1~0.005:1；
(2) the preparation of leaching liquor：Ready black tea, matrimony vine and honeysuckle are put into water and boil 80~120min, filtered off afterwards
Residue, adds white granulated sugar in filter liquor, then filters, and the solution after filtering sterilizes 15min at 121 DEG C, take out and it is cool extremely
Room temperature, obtains final product leaching liquor；
Wherein, the black tea and the mass ratio of water are 0.004~0.01:1, matrimony vine is 0.005~0.02 with the mass ratio of water:1,
Honeysuckle is 0.002~0.008 with the mass ratio of water:1, white granulated sugar is 0.04~0.07 with the mass ratio of water:1；
(3) it is inoculated with：Black tea mother bacterial liquid is added in leaching liquor after sterilization, the black tea mother bacterial liquid is with the volume ratio of leaching liquor
0.02:1~0.005:1；
(4) ferment：The liquid that will be inoculated with is positioned in 27~37 DEG C of shaking tables, is 27~37 DEG C in fermentation temperature, and shaking speed is
Fermented under conditions of 80~120r/min, fermentation time is 4~5 days, obtains final product functional black tea bacteria beverage.
3. it is according to claim 2 with matrimony vine honeysuckle black tea as raw material prepare functional black tea bacteria beverage method, its
It is characterised by：(1) middle boiling time is 80~120min to the step.
4. it is according to claim 2 with matrimony vine honeysuckle black tea as raw material prepare functional black tea bacteria beverage method, its
It is characterised by：The step is (2) middle to seal filtering using eight layers of gauze.
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| CN108812998A (en) * | 2018-04-13 | 2018-11-16 | 华侨大学 | A kind of globe artichoke black-tea fungus drink and preparation method thereof |
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| CN109757578A (en) * | 2019-03-22 | 2019-05-17 | 中国科学院过程工程研究所 | A kind of production method of perilla leaf fermented tea fermented beverage |
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| CN109757573A (en) * | 2019-03-14 | 2019-05-17 | 天水师范学院 | A kind of dark plum hoveniae semoveniae semen fermented tea composite beverage and preparation method thereof |
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