CN106718948A

CN106718948A - Improve the screening technique of the aseptic seeding method of Glycyrrhiza Seeds germination rate

Info

Publication number: CN106718948A
Application number: CN201710171875.1A
Authority: CN
Inventors: 不公告发明人
Original assignee: Individual
Current assignee: Individual
Priority date: 2017-03-22
Filing date: 2017-03-22
Publication date: 2017-05-31

Abstract

The present invention provides the screening technique of the aseptic seeding method for improving Glycyrrhiza Seeds germination rate, Seeds preprocess is adjusted to operate the physiological effect to Radix Glycyrrhizae tissue culturing seedling by using unitary variant method, it is determined that most suitable pretreatment operation and operating time, and reversely determine basal medium by determining that Seeds preprocess is operated, the increment of tissue-cultured seedling is introduced simultaneously as screening criteria, is more suitable for commercial plant metaplasia product.

Description

Improve the screening technique of the aseptic seeding method of Glycyrrhiza Seeds germination rate

Technical field

The invention belongs to Radix Glycyrrhizae technical field of tissue culture, more particularly to the aseptic seeding side for improving Glycyrrhiza Seeds germination rate The screening technique of method.

Background technology

Radix Glycyrrhizae,（Scientific name：Glycyrrhiza uralensis Fisch）Alias：State is old, Herba Hedyotis cantonensis, Glycyrrhiza Uralensis, sweet Son.Pulse family, Glycyrrhiza perennial herb, root are sturdy with root-like stock, are a kind of help Chinese herbal medicines.To a kind of good medicine of human body, Medicinal part is root and rhizome, and Medicinal Materials Characters root is long 25~100 centimetres in cylinder, 0.6~3.5 centimetre of diameter.Crust is elastic Differ, surface rufous or taupe brown.In cylinder, there is bud scar on surface to rhizome, and there is marrow at section middle part.Gas is micro-, and taste is sweet and special. Indication is clearing heat and detoxicating, expelling phlegm and arresting coughing, gastral cavity abdomen etc..Happiness is dark moist, the low dry climate of sunshine temperature long.Being grown Radix Glycyrrhizae more In arid, semiarid desert steppe, desert edge and loess hill area.Root and root-like stock hyoscine.Traditional Radix Glycyrrhizae is obtained The method of taking is excavation leaf plant resource, soil erosion and serious soil sandification is caused, simultaneously because people are devastatingly right Leaf licorice is excavated, and wild plant is almost become extinct, and the protectiveness to Radix Glycyrrhizae utilizes extremely urgent, but due to licorice planting germination Rate is not high and people are to the demand of its this life metabolin, so that the tissue cultures of Radix Glycyrrhizae are to studying and production has important work With, however existing screening technique be not suitable for industrialization large-scale production screened.

Cerium is periodic system Ι Ι Ι races subgroup lanthanide series, a kind of rare earth element.Atomic number 58.Stable isotope：136、 138、140、142.Grey metal, there is malleability.Density：Quadratic crystal 6.9, cubic crystal 6.7.799 DEG C of fusing point, boiling point 3426 ℃.Cerium is a kind of silver-gray active metal, and powder easy spontaneous combustion in atmosphere is soluble in acid.The title of cerium derives from asteroid The English name of ceres.Content of the cerium in the earth's crust about 0.0046%, is abundance highest in rare earth element.

The content of the invention

Above mentioned problem is had based on prior art, the present invention provides the aseptic seeding method of raising Glycyrrhiza Seeds germination rate Screening technique, adjusts Seeds preprocess to operate the physiological effect to Radix Glycyrrhizae tissue culturing seedling, it is determined that most by using unitary variant method Suitable pretreatment operation and operating time, and reversely determine basal medium by determining that Seeds preprocess is operated, while introducing group The increment of seedling is trained as screening criteria, commercial plant metaplasia product is more suitable for.

A kind of screening technique of the aseptic seeding method for improving Glycyrrhiza Seeds germination rate, it is comprised the following steps：

Step S10 determines preliminary screening scope, and data of literatures is consulted in retrieval, in contrast other plant species aseptic seeding Seeds preprocess is operated, and determines the preliminary screening scope of pretreatment operation；

Step S20 selects explant, and selection health, the Glycyrrhiza Seeds that growing environment is consistent and growth conditions are similar are used as explant Body, unified sterilization treatment is carried out to explant；

Step S30 control cultures, its culture medium based on MS culture mediums, the concentration of casein is 0.5g/L, activated carbon it is dense The concentration for 0.15 mg/L, cerous nitrate is spent for 3g/L, and the concentration of agar powder is 10g/L, the concentration of white granulated sugar is 7g/L, is prepared Into test culture medium, same amount of Glycyrrhiza Seeds are inoculated with respectively, and carry out conventional organization culture as a control group, in conventional training Foster basic condition carries out Seeds preprocess operation as experimental group in addition, and control group all sets multiple parallel with experimental group Group；

Step S40 record detection parameters, culture counts the quantity and the number of browning of the sprouting of each experimental group and control group after 7 days Amount, and calculate germination rate and melting brown rate；

Step S50 increments are calculated, and respectively take out the Radix Glycyrrhizae seedling of same experimental group or control group, rinse dry with clear water Only, dry surface moisture and Radix Glycyrrhizae seedling is weighed, record weight；

Step S60 calculate assessment factor, by weight amount reduce 10 times, along with germination rate subtract melting brown rate calculate assessment because Number, contrasts the assessment factor of various concentrations gradient, picks out the corresponding Seeds preprocess operation of the maximum assessment factor of numerical value；

Step S70 reduces screening scope, and step S10- is repeated using the pretreatment operation scope picked out in step S60 S60 screens the time range of pretreatment operation；

Screen basal medium, the most suitable pretreatment operation determined using step S70 and the different bases of time collocation in step S80 directions Basal culture medium, repeat step S40 and step S50, and using the computational methods calculating assessment factor of step S60, it is determined that most suitable base Basal culture medium.

Preferably, each experimental group or control group set 5-15 parallel group in described step S30.

Preferably, the number of times repeated in described step S70 is 2-3 times.

Preferably, in described step S80 different basal mediums include MS culture mediums, N6 culture mediums, B5 medium, Nitsh culture mediums, White culture mediums and Miller culture mediums.

Specific embodiment

With reference to specific embodiment, the invention will be further described.

As the presently preferred embodiments, each experimental group or control group set 10 parallel groups in described step S30.

As the presently preferred embodiments, the number of times repeated in described step S70 is 3 times.

As the presently preferred embodiments, in described step S80 different basal mediums include MS culture mediums, N6 culture mediums, B5 medium, Nitsh culture mediums, White culture mediums and Miller culture mediums.

Seeds preprocess is defined below with germination rate and growth quality higher by screening：After having gathered Glycyrrhiza Seeds The preprocess method that the dense sulfuric acid treatment 60min of mechanical friction 10min or use 98% is carried out to seed coordinates with MS culture mediums Based on culture medium, the concentration of casein is 0.5g/L, the concentration of activated carbon is that 0.15 mg/L, the concentration of cerous nitrate are 3g/L, The concentration of agar powder is the concentration of 10g/L, white granulated sugar for the sowing culture medium of 7g/L has germination rate and biological quality higher.

Claims

1. the screening technique of the aseptic seeding method of Glycyrrhiza Seeds germination rate is improved, it is characterised in that it is comprised the following steps：

2. the screening technique of the aseptic seeding method for improving Glycyrrhiza Seeds germination rate according to claim 1, its feature exists In each experimental group or control group set 5-15 parallel group in described step S30.

3. the screening technique of the aseptic seeding method for improving Glycyrrhiza Seeds germination rate according to claim 1, its feature exists In the number of times repeated in described step S70 is 2-3 times.

4. the screening technique of the aseptic seeding method for improving Glycyrrhiza Seeds germination rate according to claim 1, its feature exists In, in described step S80 different basal mediums include MS culture mediums, N6 culture mediums, B5 medium, Nitsh culture mediums, White culture mediums and Miller culture mediums.