CN102150618A - Method for quickly propagating high-quality liquorice seedlings - Google Patents
Method for quickly propagating high-quality liquorice seedlings Download PDFInfo
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- CN102150618A CN102150618A CN 201110024490 CN201110024490A CN102150618A CN 102150618 A CN102150618 A CN 102150618A CN 201110024490 CN201110024490 CN 201110024490 CN 201110024490 A CN201110024490 A CN 201110024490A CN 102150618 A CN102150618 A CN 102150618A
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Abstract
The invention relates to a method for quickly propagating high-quality liquorice seedlings. The method is realized by sequentially comprising the following steps of: firstly, washing seeds with running water, sterilizing the seeds with 75% alcohol and washing 3-4 times with sterile water; then sterilizing the seeds with 0.1% corrosive sublimate, and washing 3-4 times with sterile water; then inoculating the seeds to an MS (Murashige and Skoog) culture medium for culturing aseptic seedlings for 1 month; then cutting stalks of the aseptic seedlings; chopping the stalks into stalk sections with the length of 0.5cm; and inoculating into a 0.5mg/L MS+KH2PO310mg/L+NAA culture medium to be cultured continuously. The method for quickly propagating the high quality liquorice seedlings has the advantages that the rooting rate of the aseptic seedlings reaches 100%, aseptic liquorice nursery stocks can be quickly propagated, the cycle is short, and the method is not influenced by factors, such as seasons, weather, natural disaster and the like and can realize industrial production. By adopting the technology, many problems of low propagation capability, shortage of resources, pesticide residue and the like of a medicinal plant can be solved. Since the technology for quickly propagating aseptic liquorice seedling stalks is firstly utilized in Gansu Province, in China, novel liquorice germ plasms in Gansu can be quickly propagated and meet market requirements and have strategic meanings in the aspects of preventing wind, stabilizing sands, protecting ecological environment and germ plasm.
Description
Technical field
The present invention relates to a kind of Radix Glycyrrhizae breeding method, more specifically to the method for the quick breeding of a kind of Radix Glycyrrhizae high quality seedling.
Background technology
Radix Glycyrrhizae is one of Gansu Province's five big authentic medicinal herbses, mainly is distributed in the Hexi prefecture, is the wild sand-fixation plant of special-protection-by-the-State, is preserving the ecological environment and range resource, prevents that aspects such as desertification from playing an important role.In recent years, because Radix Glycyrrhizae sharply increases at the consumption of pharmacy industry, grocery trade and cosmetic industry etc., people excavate wild Radix Glycyrrhizae devastatingly, and wild plant is almost become extinct, and have seriously destroyed ecotope.Though our province is being obtained some achievements aspect the Radix Glycyrrhizae artificial cultivation, but artificial cultivation Radix Glycyrrhizae, generally needed for 4 years just can set seeds, and Radix Glycyrrhizae is gathered and generally only needs 2～3 years, so seed disappearance in producing region has become one big " bottleneck " that hinders the stable development of Radix Glycyrrhizae industry.But the research in Radix Glycyrrhizae scientific research field, especially breeding and the quick proliferation of seedling almost is blank.At present, the research overwhelming majority of Radix Glycyrrhizae concentrates on aspects such as cultivation, active ingredient and pharmacological effect research, and reports seldom about the Radix Glycyrrhizae tissue culture and the research of quick proliferation.Utilize research that tissue culture technique carries out Glycyrrhiza early, but mainly concentrate on other plant in the Glycyrrhiza.Studied the cultivation of glycyrrhiza inflate bat callus as people such as Liang Yuling, Kovalenko etc. have set up glycyrrhiza glabra hairy root cultivating system, and it is less to the research of Ural Radix Glycyrrhizae, only there are people such as Pei Yanxi to study the influence of plant growth regulator, and the quick proliferation of Radix Glycyrrhizae cane is also rarely had research the Radix Glycyrrhizae callus induction.
Summary of the invention
The technical problem to be solved in the present invention is: the problem that solves the breeding of our province Radix Glycyrrhizae breed breeding and breeding; solve long problem of seminal propagation cycle; a kind of Radix Glycyrrhizae high quality seedling method of breeding fast is provided; obtain high quality seedling; shorten the Radix Glycyrrhizae breeding cycle, accelerate Radix Glycyrrhizae stock breeding process, carry out the scale plant produced; satisfy the demand of market, protect wild Radix Glycyrrhizae natural resources and good natural ecological environment Radix Glycyrrhizae.
The present invention realizes by following sequential steps:
A, at first select the Radix Glycyrrhizae high quality seed to wash 2h with flowing water;
B, the seed after will washing be with 75% alcohol disinfecting 1min, then with the seed behind the alcohol disinfecting with aseptic water washing 3～4 times;
C, with the seed behind the aseptic water washing again with 0.1% mercuric chloride sterilization 7min, then with the seed after the mercury sterilization with aseptic water washing 3～4 times;
D, will be inoculated in through the seed of sterilization treatment in the MS medium and cultivate aseptic seedling at last, cultivation temperature is 26 ℃～28 ℃, and intensity of illumination is 1500LX～2000LX, and light application time is 18h/d～24h/d;
E, cultivation after 1 month are cut the cane of aseptic seedling, are cut into the stem section of 0.5cm length, are inoculated in MS+KH
2PO
4Carry out the numerous cultivation of quick expansion of aseptic seedling in the 10mg/L+NAA 0.5mg/L medium.
The present invention utilizes method for tissue culture to breed the cane of Ural Radix Glycyrrhizae fast, and its advantage is: make the aseptic seedling rooting rate reach 100%, can breed the aseptic nursery stock of Radix Glycyrrhizae fast.Cycle is short, is not subjected to the influence of factors such as season, weather, natural calamity, and can realizes batch production production.Utilize this technology can solve problems such as the fertility of medicinal plant is low, scarcity of resources, residue of pesticide.Our Gansu is to adopt plant tissue culture technique first on the other hand, and this breeds fast to Glycyrrhiza kaasuensis Chang et Peng new germ plasm resource, not only can meet the need of market, but also check winds and fix drifting sand, preserving the ecological environment and protecting aspects such as germplasm to have strategic importance.
Embodiment
Embodiment 1
The present invention has following sequential steps:
A, at first select Ural Radix Glycyrrhizae high quality seed to wash 2h with flowing water.
B, the seed after will washing be with 75% alcohol disinfecting 1min, then with the seed behind the alcohol disinfecting with aseptic water washing 3～4 times.
C, with the seed behind the aseptic water washing again with 0.1% mercuric chloride sterilization 7min, then with the seed after the mercury sterilization with aseptic water washing 3～4 times.
D, will be inoculated in through the seed of sterilization treatment in the MS medium and cultivate aseptic seedling at last, cultivation temperature is 26 ℃, and intensity of illumination is 1500LX, and light application time is 18h/d.
E, cultivation after 1 month are cut the cane of aseptic seedling, are cut into the stem section of 0.5cm length, insert MS+KH
2PO
4Proceed the numerous cultivation of quick expansion of aseptic seedling in the 10mg/L+NAA 0.5mg/L medium.
Embodiment 2
Embodiment 2 is identical with the method for embodiment 1, and the cultivation temperature of aseptic seedling in the steps d that different is is 27 ℃, and intensity of illumination is 1800LX, and light application time is 20h/d.
Embodiment 3
Embodiment 3 is identical with the method for embodiment 1, and the cultivation temperature of aseptic seedling in the steps d that different is is 28 ℃, and intensity of illumination is 2000LX, and light application time is 24h/d.
In sum: this technology makes the aseptic seedling rooting rate reach 100%, can breed the aseptic nursery stock of Radix Glycyrrhizae fast.Gansu is to adopt the quick multiplication technique of Radix Glycyrrhizae aseptic seedling cane first, and this breeds fast to Glycyrrhiza kaasuensis Chang et Peng new germ plasm resource, not only can meet the need of market, but also check winds and fix drifting sand, preserving the ecological environment and protecting aspects such as germplasm to have strategic importance.
Claims (1)
1. the Radix Glycyrrhizae high quality seedling method of breeding fast, this method has following sequential steps:
A, at first select the Radix Glycyrrhizae high quality seed to wash 2h with flowing water;
B, the seed after will washing be with 75% alcohol disinfecting 1min, then with the seed behind the alcohol disinfecting with aseptic water washing 3～4 times;
C, with the seed behind the aseptic water washing again with 0.1% mercuric chloride sterilization 7min, then with the seed after the mercury sterilization with aseptic water washing 3～4 times;
D, will be inoculated in through the seed of sterilization treatment in the MS medium and cultivate aseptic seedling at last, cultivation temperature is 26 ℃～28 ℃, and intensity of illumination is 1500LX～2000LX, and light application time is 18h/d～24h/d;
E, cultivation after 1 month are cut the cane of aseptic seedling, are cut into the stem section of 0.5cm length, insert MS+KH
2PO
4Carry out the numerous cultivation of quick expansion of aseptic seedling in the 10mg/L+NAA 0.5mg/L medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2011100244905A CN102150618B (en) | 2011-01-21 | 2011-01-21 | Method for quickly propagating high-quality liquorice seedlings |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011100244905A CN102150618B (en) | 2011-01-21 | 2011-01-21 | Method for quickly propagating high-quality liquorice seedlings |
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| CN102150618A true CN102150618A (en) | 2011-08-17 |
| CN102150618B CN102150618B (en) | 2012-06-27 |
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Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103120085A (en) * | 2011-11-18 | 2013-05-29 | 陈芳 | Seedling transplantation method of ecological restoration type liquorices |
| CN104521760A (en) * | 2015-01-14 | 2015-04-22 | 甘肃农业大学 | Method for doubling glycyrrhiza pallidiflora maxim chromosomes through tissue culture |
| CN104686345A (en) * | 2015-02-24 | 2015-06-10 | 陈桂容 | Tissue culture rapid propagation method of liquorice |
| CN106614001A (en) * | 2017-03-22 | 2017-05-10 | 陈志育 | Screening method of light source conditions for induction culture of liquorice cotyledon |
| CN106614000A (en) * | 2017-03-22 | 2017-05-10 | 陈志育 | Screening method of glycyrrhiza uralensis rooting medium containing aspirin |
| CN106665369A (en) * | 2017-03-22 | 2017-05-17 | 陈志育 | High-inductivity induction culture medium for cotyledons of radix glycyrrhizae |
| CN106718948A (en) * | 2017-03-22 | 2017-05-31 | 陈志育 | Improve the screening technique of the aseptic seeding method of Glycyrrhiza Seeds germination rate |
| CN106797891A (en) * | 2017-03-22 | 2017-06-06 | 陈志育 | A kind of Radix Glycyrrhizae method for tissue culture for promoting root |
| CN106879469A (en) * | 2017-03-22 | 2017-06-23 | 陈志育 | It is a kind of to screen the method for promoting Callus Tissue of Glycyrrhiza Multiplying culture condition |
| CN106888975A (en) * | 2017-03-22 | 2017-06-27 | 陈志育 | A kind of Radix Glycyrrhizae cotyledon induced tissue cultural method |
| CN106888973A (en) * | 2017-03-22 | 2017-06-27 | 陈志育 | A kind of Radix Glycyrrhizae root media containing aspirin |
| CN106888972A (en) * | 2017-03-22 | 2017-06-27 | 陈志育 | A kind of Radix Glycyrrhizae aseptic seeding culture medium of germination rate high |
| CN106900556A (en) * | 2017-03-22 | 2017-06-30 | 陈志育 | A kind of Radix Glycyrrhizae aseptic seeding method of germination rate high |
| CN106912383A (en) * | 2017-03-22 | 2017-07-04 | 陈志育 | A kind of Thidiazuron Radix Glycyrrhizae proliferated culture medium screening technique |
| CN106912382A (en) * | 2017-03-22 | 2017-07-04 | 陈志育 | A kind of screening technique of the condition of tissue culture for promoting Radix Glycyrrhizae to go out root |
| CN106912381A (en) * | 2017-03-22 | 2017-07-04 | 陈志育 | A kind of germination rate Radix Glycyrrhizae aseptic seeding Screening of Media method high |
| CN106922533A (en) * | 2017-03-22 | 2017-07-07 | 陈志育 | A kind of Radix Glycyrrhizae cultural method based on blue green light combination enhancing light source |
| CN106942056A (en) * | 2017-03-22 | 2017-07-14 | 陈志育 | A kind of Radix Glycyrrhizae cotyledon inducing culture screening technique |
| CN106942057A (en) * | 2017-03-22 | 2017-07-14 | 陈志育 | A kind of Thidiazuron Radix Glycyrrhizae proliferated culture medium |
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| CN1653886A (en) * | 2005-03-08 | 2005-08-17 | 天津大学 | Tissue culturing method for licorice adventitious root |
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Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103120085A (en) * | 2011-11-18 | 2013-05-29 | 陈芳 | Seedling transplantation method of ecological restoration type liquorices |
| CN103120085B (en) * | 2011-11-18 | 2015-05-06 | 陈芳 | Seedling transplantation method of ecological restoration type liquorices |
| CN104521760A (en) * | 2015-01-14 | 2015-04-22 | 甘肃农业大学 | Method for doubling glycyrrhiza pallidiflora maxim chromosomes through tissue culture |
| CN104686345A (en) * | 2015-02-24 | 2015-06-10 | 陈桂容 | Tissue culture rapid propagation method of liquorice |
| CN106888975A (en) * | 2017-03-22 | 2017-06-27 | 陈志育 | A kind of Radix Glycyrrhizae cotyledon induced tissue cultural method |
| CN106888973A (en) * | 2017-03-22 | 2017-06-27 | 陈志育 | A kind of Radix Glycyrrhizae root media containing aspirin |
| CN106665369A (en) * | 2017-03-22 | 2017-05-17 | 陈志育 | High-inductivity induction culture medium for cotyledons of radix glycyrrhizae |
| CN106718948A (en) * | 2017-03-22 | 2017-05-31 | 陈志育 | Improve the screening technique of the aseptic seeding method of Glycyrrhiza Seeds germination rate |
| CN106797891A (en) * | 2017-03-22 | 2017-06-06 | 陈志育 | A kind of Radix Glycyrrhizae method for tissue culture for promoting root |
| CN106879469A (en) * | 2017-03-22 | 2017-06-23 | 陈志育 | It is a kind of to screen the method for promoting Callus Tissue of Glycyrrhiza Multiplying culture condition |
| CN106614001A (en) * | 2017-03-22 | 2017-05-10 | 陈志育 | Screening method of light source conditions for induction culture of liquorice cotyledon |
| CN106614000A (en) * | 2017-03-22 | 2017-05-10 | 陈志育 | Screening method of glycyrrhiza uralensis rooting medium containing aspirin |
| CN106888972A (en) * | 2017-03-22 | 2017-06-27 | 陈志育 | A kind of Radix Glycyrrhizae aseptic seeding culture medium of germination rate high |
| CN106900556A (en) * | 2017-03-22 | 2017-06-30 | 陈志育 | A kind of Radix Glycyrrhizae aseptic seeding method of germination rate high |
| CN106912383A (en) * | 2017-03-22 | 2017-07-04 | 陈志育 | A kind of Thidiazuron Radix Glycyrrhizae proliferated culture medium screening technique |
| CN106912382A (en) * | 2017-03-22 | 2017-07-04 | 陈志育 | A kind of screening technique of the condition of tissue culture for promoting Radix Glycyrrhizae to go out root |
| CN106912381A (en) * | 2017-03-22 | 2017-07-04 | 陈志育 | A kind of germination rate Radix Glycyrrhizae aseptic seeding Screening of Media method high |
| CN106922533A (en) * | 2017-03-22 | 2017-07-07 | 陈志育 | A kind of Radix Glycyrrhizae cultural method based on blue green light combination enhancing light source |
| CN106942056A (en) * | 2017-03-22 | 2017-07-14 | 陈志育 | A kind of Radix Glycyrrhizae cotyledon inducing culture screening technique |
| CN106942057A (en) * | 2017-03-22 | 2017-07-14 | 陈志育 | A kind of Thidiazuron Radix Glycyrrhizae proliferated culture medium |
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