CN106718915A - A kind of Potato Shoot-tips culture medium, preparation method and applications - Google Patents
A kind of Potato Shoot-tips culture medium, preparation method and applications Download PDFInfo
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- CN106718915A CN106718915A CN201611209711.5A CN201611209711A CN106718915A CN 106718915 A CN106718915 A CN 106718915A CN 201611209711 A CN201611209711 A CN 201611209711A CN 106718915 A CN106718915 A CN 106718915A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to the regeneration field of plant tissue culture technique, and in particular to a kind of Potato Shoot-tips culture medium, preparation method and applications, described culture medium prescription is：0.8MS culture mediums, 150 250mg/L KNO3、40‑100mg/L Ca(H2PO4)2·H2O, 0.1 0.3mg/L solanines, 0.2 0.4mg/L heteroauxins, the benzyl purines of 0.2 0.5mg/L 6,0.05 0.2mg/L gibberellic acid, 0.5 1mg/L glutathione, 25 35g/L sucrose and 8 10g/L agar, the present invention enriches the nutriment of culture medium, improve the quality of potato plant, promote the speed of growth of potato, reduce growing-seedling period, cost is reduced, is conducive to improving detoxification efficiency.
Description
Technical field
The invention belongs to the regeneration field of plant tissue culture technique, and in particular to a kind of Potato Shoot-tips culture medium,
Preparation method and applications.
Background technology
Due to the accumulation from generation to generation of the pollution of environment or virus, substantial amounts of virus is deposited in present plant, led
Causing pathological changes of plant influences the quality and quantity of product；If consumption plant, the health of people will be had a strong impact on, wherein, belong to
The plant of vegetative propagation, is highly prone to the infection of virus in growth course, for example, Potyvirus kind up to more than 30 is infected,
And over time, the generations of heredity of plant, infecting Potyvirus species can be more and more, present potato
Virus has become the problem of global generality, has a strong impact on the development of agricultural.
At present, the general chemical classes medicament of people is only effective to disease caused by bacterium or fungi, but virus is soaked
The plant action of dye is minimum, therefore, culture virusfree plant seedling has important practical significance and application value.Conventional preventing and treating
Viral methods are Physical, i.e., processed by X-ray, ultraviolet and high temperature etc., reach the purpose of passivation virus, but,
Physical belongs to small probability antivirus in virus is prevented and treated, and also causes injury to plant simultaneously, for example：Gene mutation or
Tissue damaged etc..Also chemical method antivirus, is killed virus using the medicine of suppressing virus replication, but simultaneously can be to host tissue
Injury is caused, generalization is poor.The method commonly used in industry is biological method, i.e., seed, callus or stem apex are entered
Row culture, but seed culture is effective just for generative propagation, and callus tissue culture easily makes a variation, therefore, conventional means are stem
Sharp virus-free culture, and cycle is short, the advantages of survival rate is high.
The detoxification principle of Shoot Tip Culture be due to stem apex Shi Xin growing tissues, or hormone-content difference, cause virus
The probability of infection is small, and content is low, therefore, Shoot Tip Culture has become the main path for solving Potyvirus, but, stem apex training
Foster technology is required further improvement.
The Chinese patent of Publication No. 105532479A discloses a kind of Potato Shoot-tips differential medium and preparation method.
Contain L- NAC hydrochlorides in the formula of the culture medium, calculated with 1L culture mediums, the L- NACs hydrochloride
Content is 0.1mg.The formula 1. or 2. for, 1.：0.1mg/L+ is red for MS culture mediums+heteroauxin 0.1mg/L+6- benzyl purines
Mould sour 0.1mg/L+L- NACs hydrochloride 0.1mg/L+30g/L sucrose+5-7g/L agar powders；②：MS culture mediums+NAA
0.1mg/L+6- benzyl purine 0.1mg/L+ gibberellic acid 0.1mg/L+L- NAC hydrochloride 0.1mg/L+30g/L sucrose+5-
7g/L agar powders.The invention adds L-cysteine hydrochloride in potato tissue culture with culture medium (MS culture mediums) is middle, used as anti-
Oxygen agent, reduces browning, to increase the planting percent of Potato Shoot-tips separate living tissue.But 1 is used during Shoot Tip Culture
The MS culture medium ammonium nitrogen too high levels of individual concentration, and other nutriments are also added into, can make to grow speed in Shoot Tip Culture
Degree is slow, and L-cysteine hydrochloride is easily decomposed under illumination condition, destroys the electrolyte balance of culture medium, moreover,
Decompose the material for producing not knowing, the development of plant may be influenceed.
The content of the invention
In order to further improve prior art, promote the speed of growth and emergence rate of Shoot Tip Culture, the invention discloses one
Plant Potato Shoot-tips culture medium and its application.The present invention is the further improvement to MS culture mediums, is allowed to more adapt to potato haulm
The culture of point.
Technical solution of the present invention is as follows：
A kind of Potato Shoot-tips culture medium, described culture medium prescription is：0.8MS culture mediums, 150-250mg/L KNO3、
40-100mg/L Ca(H2PO4)2·H2O, 0.1-0.3mg/L solanine, 0.2-0.4mg/L heteroauxins, 0.2-0.5mg/L
6- benzyl purines, 0.05-0.2mg/L gibberellic acid, 0.5-1mg/L glutathione, 25-35g/L sucrose and 8-10g/L agar..
Further, described culture medium prescription is：0.8MS culture mediums, 210mg/L KNO3、65mg/L Ca
(H2PO4)2·H2O, 0.15mg/L solanine, 0.3mg/L heteroauxins, 0.3mg/L 6- benzyl purines, 0.12mg/L are red mould
Acid, 0.7mg/L glutathione, 32g/L sucrose and 9g/L agar.
The proportioning of wherein MS culture mediums is：A great number of elements is：NH4NO31650, KNO31900, CaCl2·2H2O 440,
MgSO4·7H2O 370, KH2PO4170 trace elements：KI 0.83, H3BO36.2, MnSO4·4H2O 22.3, ZnSO4·7H2O
8.6, Na2MoO4·2H2O 0.25, CuSO4·5H2O 0.025, CoCl2·6H2O 0.025, molysite：FeSO4·7H2O
(27.8), Na2-EDTA·2H2O (37.3), organic substance：Inositol 100, nicotinic acid 0.5, puridoxine hydrochloride (vitamin B6) 0.5,
Thiamine hydrochloride (vitamin B1) 0.1, glycine 2.0, unit is mg/L.
Further, the invention provides the preparation method of described Potato Shoot-tips culture medium, step is
S1：It is original 0.8 times that MS culture mediums are diluted into concentration, is subsequently adding KNO3、Ca(H2PO4)2·H2O, black nightshade
Alkali, heteroauxin, 6- benzyl purines, gibberellic acid, glutathione, stir, and obtain liquid A；
S2：Take liquid A obtained in 1/3 step S1 to boil in the small aluminum pot of people, then sucrose, agar inclined in the small aluminum pot of people,
Heat while stirring, untill all thawing is so clear that you can see the bottom to agar, be subsequently poured into remaining liquid A, stir, obtain glue
Shape thing B；
S3：The pH value of the jelly B obtained using the NaOH or HCl solution regulating step S2 of 1M is to 5.8,115-125 DEG C
Steam sterilizing, sterilizes 15-25 minutes, is down to room temperature, obtains final product.
Further, the mixing speed in step S1 and S2 is 45-60r/min.
Further, application of the described Potato Shoot-tips culture medium in Potato Shoot-tips induced tissue culture.
Cultural method is：In an aseptic environment, the spire for being grown in cone periphery is peelled off on the Potato Shoot-tips from after sterilization
And phyllopodium, until exposing round and smooth growth cone.Growth cones of the 0.2-0.4mm with 1-2 piece phyllopodium is cut, is seeded in described
Culture medium on, be placed in 22-25 DEG C, under the illumination condition of intensity of illumination 2200-2800LX, 12-16h/d cultivate.
Calcium dihydrogen phosphate：It is referred to as double superhosphate, is pale powder, containing effective P4O10Up to 30%~45%, be
It is more than the twice of normal superphosphate.Double superhosphate is mainly used as acid phosphate fertilizer.Calcium dihydrogen phosphate is applied to supply plant
The elements such as phosphorus, calcium, can be used as base manure, foliage top dressing, foliage spray.It is used in mixed way with nitrogenous fertilizer, there is nitrogen fixation, reduces the damage of nitrogen
Lose.Germination, root long, branch, the solid and maturation of plant can be promoted, can be used as producing the raw material of Chemical Mixed Fertilizer.The present invention is by Ca
(H2PO4)2·H2O is applied in culture medium, finds the Ca (H of the appropriate concentration of addition2PO4)2H2O, can promote the hair of stem apex
Educate, improve the speed of growth.
Solanine：It is widely present in the plants of Solanaceae such as potato, tomato and eggplant.In tomato dark green prematurity,
Contain solanine in the inside.After potato sprouting, the solanine content of its young shoot and eye part is up to 0.3%~0.5%.
Glutathione：It is to be combined by glutamic acid, cysteine and glycine, the tripeptides containing sulfydryl, with anti-oxidant
Effect and integration detoxication.Glutathione participates in biotransformation, and wherein plant growth can make the mistake that organism metabolism is produced
Polyradical meeting damage biological film, accelerates plant in body aging, especially culture medium, vulnerable, and addition glutathione can
To improve the survival rate of potato.
The present invention is diluted on the basis of MS culture mediums, then with the addition of KNO3、Ca(H2PO4)2·H2O, black nightshade
Alkali, heteroauxin, 6- benzyl purines, gibberellic acid, glutathione, sucrose and agar.Wherein dilute the purpose of MS basal mediums
It is the content for reducing ammonia salt in nitrogen source, reduces the content of ammonium nitrogen, be conducive to the growth of culture, planting percent is high；Add
KNO3On the other hand it is that, in order to supplement potassium element, appropriate raising potassium element contains partly in order to supplement nitrate nitrogen element
Amount, is conducive to germination percentage；Ca (the H of addition2PO4)2·H2O is used in mixed way with nitrogen source, there is nitrogen fixation, reduces the loss of nitrogen, and
And promote the growth of plant；The present invention is also added into a small amount of solanine, is widely present in the young shoot of potato, and experiment is proved
Adding a small amount of solanine can promote the emergence rate of potato；The glutathione of addition is conducive to improving the resistance of plant,
The vigorous vitality of plant is kept, and reduces the browning of callus.
Compared with prior art, the present invention has advantages below：
1st, the Potato Shoot-tips culture medium that the present invention is provided is the improvement in MS basal mediums, enriches the battalion of culture medium
Material is supported, the quality of potato is improve.
2nd, detoxification of the culture medium that the present invention is provided to potato has good effect, further improves potato
The quality of seedling.
3rd, culture medium of the present invention promotes the speed of growth of potato, reduces growing-seedling period, reduces cost, is conducive to
Improve detoxification efficiency.
Specific embodiment
By specific examples below, the invention will be further described, but is not limitation of the present invention.The present invention
The MS culture mediums of the Sigma that culture medium is supplied purchased from Beijing Baeyer enlightening Bioisystech Co., Ltd.
Embodiment 1
A kind of Potato Shoot-tips culture medium, described culture medium prescription is：0.8MS culture mediums, 210mg/L KNO3、
65mg/L Ca(H2PO4)2·H2O, 0.15mg/L solanine, 0.3mg/L heteroauxins, 0.3mg/L 6- benzyl purines,
0.12mg/L gibberellic acid, 0.7mg/L glutathione, 32g/L sucrose and 9g/L agar.
The preparation method of the culture medium is that step is：
S1：It is original 0.8 times that MS culture mediums are diluted into concentration, is subsequently adding KNO3、Ca(H2PO4)2·H2O, black nightshade
Alkali, heteroauxin, 6- benzyl purines, gibberellic acid, glutathione, stir, and mixing speed is 50r/min, obtains liquid A；
S2：Take liquid A obtained in 1/3 step S1 to boil in the small aluminum pot of people, then sucrose, agar inclined in the small aluminum pot of people,
Heat while stirring, mixing speed is 50r/min, untill all thawing is so clear that you can see the bottom to agar, be subsequently poured into remaining
Liquid A, stirs, and obtains jelly B；
S3：The pH value of the jelly B obtained using the NaOH or HCl solution regulating step S2 of 1M to 5.8,120 DEG C of steam
Sterilizing, sterilizes 20 minutes, is down to room temperature, obtains final product.
Embodiment 2
A kind of Potato Shoot-tips culture medium, described culture medium prescription is：0.8MS culture mediums, 150mg/L KNO3、
40mg/L Ca(H2PO4)2·H2O, 0.3mg/L solanine, 0.2mg/L heteroauxins, 0.5mg/L 6- benzyl purines,
0.05mg/L gibberellic acid, 1mg/L glutathione, 25g/L sucrose and 10g/L agar.
The preparation method of the culture medium is similar to Example 1.
Embodiment 3
A kind of Potato Shoot-tips culture medium, described culture medium prescription is：0.8MS culture mediums, 250mg/L KNO3、
100mg/L Ca(H2PO4)2·H2O, 0.1mg/L solanine, 0.4mg/L heteroauxins, 0.2mg/L 6- benzyl purines,
0.2mg/L gibberellic acid, 0.5mg/L glutathione, 35g/L sucrose and 8g/L agar.
The preparation method of the culture medium is similar to Example 1.
Comparative example 1
A kind of Potato Shoot-tips culture medium, described culture medium prescription is：0.8MS culture mediums, 210mg/L KNO3、
65mg/L Ca(H2PO4)2·H2O, 0.15mg/L solanine, 0.3mg/L heteroauxins, 0.3mg/L 6- benzyl purines,
0.12mg/L gibberellic acid, 0.85mg/L L- NACs hydrochloride, 32g/L sucrose and 9g/L agar.
Compared with Example 1, difference is that glutathione is replaced with into L- NAC hydrochlorides.
The preparation method of the culture medium is similar to Example 1.
Test example 1
Culture medium：Embodiment 1-3, Potato Shoot-tips culture medium obtained in comparative example 1.
In an aseptic environment, from after sterilization peelled off on potato (middle potato 5) stem apex be grown in cone periphery spire and
Phyllopodium, until exposing round and smooth growth cone.0.3mm is cut with 2 growth cones of phyllopodium, described culture medium is seeded in
On, 24 DEG C are placed in, cultivated under the illumination condition of intensity of illumination 2500LX, 14h/d, change a subculture within 3 days, each test process
3 repetitions, each repeats 50 stem apexs, cultivates the growth performance of 50 days observation planting percents and bud, and concrete outcome is as shown in table 1.
The stem apex upgrowth situation of table 1
From table 1 it follows that the emergence rate of embodiment of the present invention 1-3 medium cultures has reached more than 93%, seedling
28.5-32 days time, more than 91%, seedling leaves are dark green, stem is healthy and strong, without aetiolation for transplanting survival rate, planted after transplanting
Strain is healthy and strong, root system quality is good, illustrates to be conducive to the Shoot Tip Culture of potato by the MS culture mediums after improvement, and with comparative example 1
Compare, glutathione is replaced with into L- NAC hydrochlorides, the seedling time is slightly shorter, and transplanting survival rate is slightly below this hair
Bright, plant quality is also slightly worse, and reason is probably the photo-labile of L- NAC hydrochlorides.
In sum, the present invention enriches the nutriment of culture medium, improves the quality of potato plant, promotes horse
The speed of growth of bell potato, reduces growing-seedling period, reduces cost, is conducive to improving detoxification efficiency.
Above the present invention is described in detail with a general description of the specific embodiments, but in the present invention
On the basis of, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore, not
Deviate these modifications or improvements on the basis of present invention spirit, belong to the scope of protection of present invention.
Claims (5)
1. a kind of Potato Shoot-tips culture medium, it is characterised in that described culture medium prescription is：0.8MS culture mediums, 150-
250mg/L KNO3、40-100mg/L Ca(H2PO4)2·H2O, 0.1-0.3mg/L solanine, 0.2-0.4mg/L heteroauxins,
0.2-0.5mg/L 6- benzyl purines, 0.05-0.2mg/L gibberellic acid, 0.5-1mg/L glutathione, 25-35g/L sucrose and 8-
10g/L agar.
2. Potato Shoot-tips culture medium according to claim 1, it is characterised in that described culture medium prescription is：0.8MS
Culture medium, 210mg/L KNO3、65mg/L Ca(H2PO4)2·H2O, 0.15mg/L solanine, 0.3mg/L heteroauxins,
0.3mg/L 6- benzyl purines, 0.12mg/L gibberellic acid, 0.7mg/L glutathione, 32g/L sucrose and 9g/L agar.
3. the preparation method of Potato Shoot-tips culture medium according to claim 1 and 2, it is characterised in that step is：
S1：It is original 0.8 times that MS culture mediums are diluted into concentration, is subsequently adding KNO3、Ca(H2PO4)2·H2O, solanine, Yin
Indolylbutyric acid, 6- benzyl purines, gibberellic acid, glutathione, stir, and obtain liquid A；
S2：Take liquid A obtained in 1/3 step S1 to boil in the small aluminum pot of people, then sucrose, agar are inclined in the small aluminum pot of people, Bian Jia
Hot side stirring, untill all thawing is so clear that you can see the bottom to agar, is subsequently poured into remaining liquid A, stirs, and obtains jelly
B；
S3：The pH value of the jelly B obtained using the NaOH or HCl solution regulating step S2 of 1M is to 5.8,115-125 DEG C of steam
Sterilizing, sterilizes 15-25 minutes, is down to room temperature, obtains final product.
4. the preparation method of Potato Shoot-tips culture medium according to claim 3, it is characterised in that in step S1 and S2
Mixing speed is 45-60r/min.
5. application of the Potato Shoot-tips culture medium according to claim 1 and 2 in Potato Shoot-tips induced tissue culture.
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| CN201611209711.5A CN106718915A (en) | 2016-12-23 | 2016-12-23 | A kind of Potato Shoot-tips culture medium, preparation method and applications |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115088615A (en) * | 2022-07-21 | 2022-09-23 | 北京市农林科学院 | Method for improving doubling efficiency of corn haploid by glutathione |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102428868A (en) * | 2011-09-16 | 2012-05-02 | 李庆伟 | Development of novel potato test-tube plantlet culture medium |
| CN105432689A (en) * | 2016-01-27 | 2016-03-30 | 时启梅 | Traditional Chinese medicine disinfectant and preparation method thereof |
| CN105532479A (en) * | 2016-02-04 | 2016-05-04 | 内蒙古农业大学 | Potato stem tip differentiation medium and preparation method thereof |
| CN105794638A (en) * | 2016-03-19 | 2016-07-27 | 北华大学 | Medium composition for preventing magnolia sieboldii bud tissue culture browning and method |
-
2016
- 2016-12-23 CN CN201611209711.5A patent/CN106718915A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102428868A (en) * | 2011-09-16 | 2012-05-02 | 李庆伟 | Development of novel potato test-tube plantlet culture medium |
| CN105432689A (en) * | 2016-01-27 | 2016-03-30 | 时启梅 | Traditional Chinese medicine disinfectant and preparation method thereof |
| CN105532479A (en) * | 2016-02-04 | 2016-05-04 | 内蒙古农业大学 | Potato stem tip differentiation medium and preparation method thereof |
| CN105794638A (en) * | 2016-03-19 | 2016-07-27 | 北华大学 | Medium composition for preventing magnolia sieboldii bud tissue culture browning and method |
Non-Patent Citations (1)
| Title |
|---|
| 赵云峰等: "茄子采后生理及贮藏保鲜技术研究进展", 《食品工业科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115088615A (en) * | 2022-07-21 | 2022-09-23 | 北京市农林科学院 | Method for improving doubling efficiency of corn haploid by glutathione |
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Application publication date: 20170531 |