CN102428868A - Development of novel potato test-tube plantlet culture medium - Google Patents
Development of novel potato test-tube plantlet culture medium Download PDFInfo
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- CN102428868A CN102428868A CN2011102739512A CN201110273951A CN102428868A CN 102428868 A CN102428868 A CN 102428868A CN 2011102739512 A CN2011102739512 A CN 2011102739512A CN 201110273951 A CN201110273951 A CN 201110273951A CN 102428868 A CN102428868 A CN 102428868A
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Abstract
The present invention provides a special culture medium for potato test-tube plantlets, which belongs to the high and new technical field of agriculture. According to the special culture medium, breeding speed of the potato test-tube plantlets is accelerated and production of potato minituber is promoted. Through the comparative experiment it is discovered that nitrogen nutrition with various forms and ratios has enormous impact on growing states of the potato test-tube plantlet, and a formula of the potato test-tube plantlet culture medium is determined by analyzing the experiment design and performing production inspection. The application of ammonium nitrogen or urea only as a nitrogen source in the culture medium it is unfavorable for growth of the plantlet; the mixture of ammonium nitrogen and nitrate nitrogen in an appropriate proportion is used to replace nitrogen source in the MS culture medium; and the novel culture medium can increase the growth amount (fresh weight) of the potato test-tube plantlet by 78 per cent and increase the leaf size (length) of the potato test-tube plantlet by 16.9 per cent. The special culture medium for the potato test-tube plantlet is very effective.
Description
Technical field
this technology belongs to the agricultural high-tech field.
Background technology
potato detoxicating technology is the important channel of realizing the potato high yield, and the cultivation of potato test-tube plantlet and fast breeding are one of key technologies of the former potato seed production of toxicity-removing white potato.For a long time, produce MS or MA medium that the potato test-tube plantlet medium mainly adopts tradition to continue, though can carry out the production of potato test-tube plantlet, these medium do not aim at potato test-tube plantlet and cultivate and design.Therefore, its nutrient composition content and ratio growing of the most suitable potato test-tube plantlet not necessarily.
Summary of the invention
MS medium nutrient component and proportioning thereof have at first been studied in
, think that content of elements can satisfy the potato test-tube plantlet growth.Primary study of the present invention the influence that potato test-tube plantlet is grown of nitrogen, phosphorus, potassium, hope can be found out and be more suitable for nutrition-allocated proportion and the element morphology that potato test-tube plantlet grows, thus the special-purpose potato test-tube plantlet medium of development.
the present invention realizes through following test method.Adopt 7 kinds of culture medium prescriptions, wherein compare (CK) with the MS medium.Each culture medium prescription is seen table 1.Each medium total nitrogen content is identical with the MS medium, but sources of nitrogen is different, and trace element is formed identical with MS, and the phosphorus potassium content is variant.In the 100ml triangular flask, add the 8ml culture fluid, 10 sections of the Atlantic Ocean potato kind test-tube plantlet stipes segments in every 3 weeks of bottle graft kind cultivation, 15 bottles of every processing are cultivated under 23 ~ 25 ℃ of temperature, illumination 3000lx condition.Respectively at sampling survey plant height, the number of blade, blade size and fresh weight after inoculating 14 days, 23 days and 30 days, 3 bottles of every processing samplings.Represent the blade size with length of blade, test data is all carried out variance analysis.
number of blade and blade size are analyzed.The results of analysis of variance shows, single-strain blade quantity difference with insignificance between processing.The single-strain blade number is maximum in No. 7 medium, reaches 8.5/strain, and comparison is according to many 9%, but does not reach significance level.Explain that several kinds of medium do not have evident difference on blade forms.The plant leaf size differences is obvious in the different medium.When taking a sample for the first time in No. 4 medium plant leaf maximum, secondly be No. 2 medium, difference with insignificance between the two, but compare significant difference with other.Sampling for the second time is still the plant leaf maximum of No. 4 medium, and No. 2 medium takes second place, but on the blade size, does not all reach significance level between all medium.Then maximum when taking a sample for the third time with the blade of No. 7 medium, significantly greater than contrast and other processing.Next is No. 4 medium.
plant fresh weight and unit plant height fresh weight are analyzed.The plant fresh weight is represented plant absolute growth amount, and unit plant height fresh weight, then represents the sturdy degree of plant.Unit plant height fresh weight is big, explains that plant strain growth is sturdy.Result of the test finds out that what the average fresh weight of plant was maximum is No. 4 and No. 2 medium, all significantly greater than contrast.The plant fresh weight and the contrast difference of No. 6, No. 7 medium are not remarkable.Average unit plant height fresh weight is No. 2 medium maximums, and secondly comparison is No. 7 medium according to weighing 34.6%, is higher than contrast 15.4%, all reaches significance level.The plant height of No. 4 medium is maximum, but does not reach significance level with contrast ratio, and all reaches significance level with other several processing ratios.The plant length of internode does not have difference basically with contrast in No. 4 medium, and the plant length of internode of all the other 3 medium all is shorter than contrast, and No. 7, No. 2 medium significantly are shorter than contrast.The plant internode is short under same condition of culture, shows that plant strain growth is healthy and strong.
result show, No. 1, No. 3 medium is not suitable for the potato test-tube plantlet growth, all contains KCl 1000 mg/L and K2SO4 440mg/L in these two kinds of medium.In addition, also contain (NH4) 2SO4 4000mg/L in No. 1 medium, contain urea 2100 mg/L in No. 3 medium.It is reported, contain KCl 1000 mg/L in the potato test-tube plantlet medium, but the plant normal growth, and the content of K2SO4 can not cause plant dead in the medium.Can think that thus No. 1, No. 3 medium contain too much NH4+ and urea probably and cause the plant can not normal growth.In all the other 5 kinds of medium, best with No. 4 culture effect.Indexs such as its plant fresh weight, blade size and unit plant height fresh weight all are significantly higher than contrast MS medium, and its plant height is basic identical with contrast, and the plant fresh weight is higher than contrast, and this explains that its plant comparison is sturdy according to plant.This medium and MS medium difference are to replace the NH4NO3 among the MS with (NH4) 2SO4, and nitrate nitrogen replenishes with KNO3.No. 2 medium is made nitrogenous source with nitrate nitrogen fully, and its plant strain growth is more sturdy than No. 4 medium.The difference of this medium and MS is not contain NH4NO3, contains KNO3 6100mg/L, and other composition is identical, and this proof KNO3 can promote plant strain growth healthy and strong, shortens length of internode.
the present invention (No. 4) are adjusted into (NH4) 2SO4 2000mg/L, KNO3 3000mg/L with nitrogenous source in the MS medium; Other constituent contents are identical with the MS medium; Can make potato test-tube plantlet growth increment (fresh weight) improve 78%; Blade size (length) increases by 16.9%, can effectively improve potato test-tube plantlet and breed speed, and effect is very obvious.
Claims (2)
1. the present invention is the potato test-tube plantlet special culture media, mainly is that the nitrogen nutrition to the MS medium improves, and effect is very obvious, agrees without the applicant, requires its prescription is protected.
2. require research process is protected.
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| CN2011102739512A CN102428868A (en) | 2011-09-16 | 2011-09-16 | Development of novel potato test-tube plantlet culture medium |
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| CN2011102739512A CN102428868A (en) | 2011-09-16 | 2011-09-16 | Development of novel potato test-tube plantlet culture medium |
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| CN102428868A true CN102428868A (en) | 2012-05-02 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168697A (en) * | 2013-04-19 | 2013-06-26 | 云南农业大学 | Culture medium for delaying growth speed of potato tissue culture seedling |
| CN106718915A (en) * | 2016-12-23 | 2017-05-31 | 江西宜信堂医疗科技有限公司 | A kind of Potato Shoot-tips culture medium, preparation method and applications |
| CN112106660A (en) * | 2020-10-09 | 2020-12-22 | 山东蓬勃生物科技有限公司 | Culture medium and culture method for improving potato propagation process efficiency |
Citations (3)
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| JPH02291219A (en) * | 1989-05-01 | 1990-12-03 | Snow Brand Milk Prod Co Ltd | Potato hairy root capable of producing 5-lipoxygenase, its production and 5-lipoxygenase |
| CN1089076A (en) * | 1993-01-07 | 1994-07-13 | 天津市蔬菜研究所 | Fast propagation technique for potato using depoisoned small seed-potato |
| CN1158691A (en) * | 1997-03-10 | 1997-09-10 | 大港油田集团运输公司 | Production method and cultivation equipment for miniature detoxicated potato seeds |
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2011
- 2011-09-16 CN CN2011102739512A patent/CN102428868A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02291219A (en) * | 1989-05-01 | 1990-12-03 | Snow Brand Milk Prod Co Ltd | Potato hairy root capable of producing 5-lipoxygenase, its production and 5-lipoxygenase |
| CN1089076A (en) * | 1993-01-07 | 1994-07-13 | 天津市蔬菜研究所 | Fast propagation technique for potato using depoisoned small seed-potato |
| CN1158691A (en) * | 1997-03-10 | 1997-09-10 | 大港油田集团运输公司 | Production method and cultivation equipment for miniature detoxicated potato seeds |
Non-Patent Citations (4)
| Title |
|---|
| 《山东农业科学》 19991231 马伟青等 "培养基中氮磷钾对马铃薯试管苗生长的影响" 第36-37页 1-2 , 第4期 * |
| 《甘肃农业科技》 20031231 王正新 "马铃薯脱毒试管苗简易培养研究" 第14-17页 1-2 , 第6期 * |
| 王正新: ""马铃薯脱毒试管苗简易培养研究"", 《甘肃农业科技》, no. 6, 31 December 2003 (2003-12-31), pages 14 - 17 * |
| 马伟青等: ""培养基中氮磷钾对马铃薯试管苗生长的影响"", 《山东农业科学》, no. 4, 31 December 1999 (1999-12-31), pages 36 - 37 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168697A (en) * | 2013-04-19 | 2013-06-26 | 云南农业大学 | Culture medium for delaying growth speed of potato tissue culture seedling |
| CN106718915A (en) * | 2016-12-23 | 2017-05-31 | 江西宜信堂医疗科技有限公司 | A kind of Potato Shoot-tips culture medium, preparation method and applications |
| CN112106660A (en) * | 2020-10-09 | 2020-12-22 | 山东蓬勃生物科技有限公司 | Culture medium and culture method for improving potato propagation process efficiency |
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Application publication date: 20120502 |