CN101946712A - Algae breeding method based on intergeneric hybridization - Google Patents
Algae breeding method based on intergeneric hybridization Download PDFInfo
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- CN101946712A CN101946712A CN2010102718545A CN201010271854A CN101946712A CN 101946712 A CN101946712 A CN 101946712A CN 2010102718545 A CN2010102718545 A CN 2010102718545A CN 201010271854 A CN201010271854 A CN 201010271854A CN 101946712 A CN101946712 A CN 101946712A
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- marine alga
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- algae
- breeding method
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Abstract
The invention discloses an algae breeding method based on intergeneric hybridization, which is characterized by using temperature, illumination and other physical conditions and chemical elements in culture solution, such as nitrogen, phosphorus, iron and the like to treat algae gametophyte cells of different genera of Laminariales; synchronously developing different genera of female gametophytes and male gametophytes to form intergeneric heterozygotes; and developing the intergeneric heterozygotes to large sporophytes. The method has the advantages that great differences of forms, yields and economic components (algin, polysaccharides from Dunaliella salina) of different genera of algaes are utilized to culture artificial algae with the comprehensive advantages of two genera of algaes through intergeneric hybridization, thus obtaining novel algae varieties or strains having the advantage of comprehensive economic characters, and improving economic attributes of yield, economic components and the like.
Description
Technical field
The present invention relates to a kind of breeding algae method, specifically a kind of marine alga breeding method based on intergeneric cross.
Background technology
There is not natural intergeneric cross marine alga in occurring in nature.The marine alga Hybrid that present marine alga breeding work is cultivated also all is in planting or the kind or the strain of interspecific cross.Although crossbreeding technology is used widely at present, but its breeding material all is the exchange or the combination of genetic material in being confined to belong to, the marine alga economic characters of its acquisition are still keeping the characteristic of this genus marine alga, especially can't be significantly improved for proterties such as output, economic sectors, the space of further improveing the marine alga economic characters on this basis is limited.
Summary of the invention
At the problems referred to above, the purpose of this invention is to provide a kind of marine alga breeding method based on intergeneric cross, cultivation has two artificial species of seaweed that belong to the marine alga overall merit, thereby obtains to have the marine alga new varieties or the new lines of integral economic trait advantage, improves its output and economic worth.
The objective of the invention is to be achieved through the following technical solutions: a kind of marine alga breeding method based on intergeneric cross, it is characterized in that utilizing the chemical element in physical condition and the culture fluid to handle the marine alga gametophyte cell that Laminariales do not belong to together, make that different genus is female, male gametophyte is grown synchronously, form the intergeneric cross zygote, and growth is large-scale sporophyte (thallus).
Described physical condition comprises temperature and illumination, is specially temperature 8-16 ℃, and intensity of illumination is 1000～4000lx, and periodicity of illumination is 8～16h.
Chemical element in the described culture fluid comprises nitrogen, phosphorus, iron, and concrete content is nitrate nitrogen content 20～80mg/L, and phosphate content is 2～5 mg/L, and divalent iron salt content is 5～20 mg/L.
The invention has the advantages that utilization does not belong to marine alga form, output, economic sector (algin, salt polysaccharides) the huge situation of difference together, pass through intergeneric cross, cultivation has two artificial species of seaweed that belong to the marine alga overall merit, thereby obtain to have the marine alga new varieties or the new lines of integral economic trait advantage, improve economic attributions such as output, economic sector.
Embodiment
Further specify the present invention below by specific embodiment.
Embodiment below by Laminariaceae Larminaria (Laminaria) marine alga and the breeding of Alariaceae undaria (Undaria) marine alga intergeneric cross further specifies the present invention.
The experiment material of present embodiment is the male gamete somatic cell of sea-tangle (Laminaria japonica) female gamete somatic cell and undaria pinnitafida (Undaria pinnatifida).Its acquisition mode is after collecting seedling by the kelp spore body of maturation isolate in the laboratory female, male gametophyte, the cell-line that obtains by cell culture technology then.
Present embodiment mainly is to be target to cultivate sea-tangle (Laminaria japonica) and undaria pinnitafida (Undaria pinnatifida) intergeneric cross kind.
The pulverizing of gametophyte cell-line is the cell branch of segment (about 1-3 cell), according to the male and female cell proportion is that 1:1～1:3 mixes, condition of culture is: temperature 8-16 ℃, intensity of illumination is 1000～4000lx, periodicity of illumination is 8～16h, and culture fluid is the sterilization seawater, and the chemical element content in the culture fluid is nitrate nitrogen content 20～80mg/L, phosphate content is 2～5 mg/L, and divalent iron salt content is 5～20 mg/L.Cultivate after 7-14 days, female and male gametophytes produces anthreid and egg capsule, arrange essence synchronously after, can form the intergeneric cross zygote, and then grow and be intergeneric cross sporophyte (thallus).
The thallus seedling of cultivating is carried out the mariculture experiment, observe its trait expression; Be the check cross reliability simultaneously, cultivate kelp synchronously (Laminaria japonica) and undaria pinnitafida (Undaria pinnatifida) compare.Culture through 5～10 months, observe ripe sporophyte, carry out the proterties comparative analysis, the sporophyte that morphological characters is separated between the graceful algae between sea-tangle and thick leaf is intergeneric cross sporophyte (thallus).
The intergeneric cross marine alga that utilizes the present technique method to obtain, its integral economic trait is better than the parent, is mainly reflected in aspects such as yield traits and economic sector height.
Some features that are appreciated that the foregoing description can change, and for example, experiment material can be chosen sea-tangle male gametophyte and undaria pinnitafida female gamete somatic cell, and perhaps experiment material can be chosen female gamete body clone or male gametophyte clone.
Claims (5)
1. marine alga breeding method based on intergeneric cross, it is characterized in that utilizing the chemical element in physical condition and the culture fluid to handle the marine alga gametophyte cell that Laminariales do not belong to together, make that different genus is female, male gametophyte is grown synchronously, form the intergeneric cross zygote, and grow and be large-scale sporophyte.
2. marine alga breeding method according to claim 1 is characterized in that described physical condition comprises temperature and illumination.
3. marine alga breeding method according to claim 1 is characterized in that the chemical element in the described culture fluid comprises nitrogen, phosphorus, iron.
4. side marine alga method of breeding according to claim 1 and 2 is characterized in that wherein said physical condition is temperature 8-16 ℃, and intensity of illumination is 1000～4000lx, and periodicity of illumination is 8～16h.
5. according to claim 1 or 3 described marine alga breeding methods, it is characterized in that the chemical element content in the wherein said culture fluid is nitrate nitrogen content 20～80mg/L, phosphate content is 2～5 mg/L, and divalent iron salt content is 5～20 mg/L.
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| CN2010102718545A CN101946712A (en) | 2010-09-03 | 2010-09-03 | Algae breeding method based on intergeneric hybridization |
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| CN2010102718545A CN101946712A (en) | 2010-09-03 | 2010-09-03 | Algae breeding method based on intergeneric hybridization |
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| CN2010102718545A Pending CN101946712A (en) | 2010-09-03 | 2010-09-03 | Algae breeding method based on intergeneric hybridization |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103651101A (en) * | 2013-12-06 | 2014-03-26 | 中国海洋大学 | Novel hybrid variety of Saccharinajaponica and Costariacostata |
| CN110622850A (en) * | 2019-11-07 | 2019-12-31 | 温州市洞头区水产科学技术研究所 | Sexual reproduction artificial crossbreeding method for fine purified variety and wild variety of sargassum fusiforme |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731141A (en) * | 2009-11-30 | 2010-06-16 | 大连水产学院 | Hybridization breeding method of undaria pinnatifida and sea tangle |
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2010
- 2010-09-03 CN CN2010102718545A patent/CN101946712A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731141A (en) * | 2009-11-30 | 2010-06-16 | 大连水产学院 | Hybridization breeding method of undaria pinnatifida and sea tangle |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103651101A (en) * | 2013-12-06 | 2014-03-26 | 中国海洋大学 | Novel hybrid variety of Saccharinajaponica and Costariacostata |
| CN110622850A (en) * | 2019-11-07 | 2019-12-31 | 温州市洞头区水产科学技术研究所 | Sexual reproduction artificial crossbreeding method for fine purified variety and wild variety of sargassum fusiforme |
| CN110622850B (en) * | 2019-11-07 | 2021-08-20 | 温州市洞头区水产科学技术研究所 | Sexual reproduction artificial crossbreeding method for fine purified variety and wild variety of sargassum fusiforme |
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Application publication date: 20110119 |