CN106717234B - A method of improving artificial culture mosses skinning drought-resistant ability - Google Patents
A method of improving artificial culture mosses skinning drought-resistant ability Download PDFInfo
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- CN106717234B CN106717234B CN201611191838.9A CN201611191838A CN106717234B CN 106717234 B CN106717234 B CN 106717234B CN 201611191838 A CN201611191838 A CN 201611191838A CN 106717234 B CN106717234 B CN 106717234B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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Abstract
The present invention relates to a kind of methods improving husky area artificial culture mosses skinning field drought-resistant ability.In laboratory conditions, select well-developed artificial culture moss (Bryum argenteumHedw.), by the cycle drought hardening for controlling water-arid-rehydration-recovery, the osmotic adjustment concentration for improving artificial culture moss body, improves its drought-resistant ability, quickly improves the ecological environment for large area of the artificial culture skinning in arid and semi-arid area and provides technical support.The present invention is easy to operate, at low cost, time-consuming short, can preferably be promoted in production application.
Description
Technical field
The invention belongs to field of environment engineering technology, specially a kind of method of artificial culture mosses skinning drought-resistant ability.
Background technology
In Arid&semi-arid area, biological breadcrust plays important in weather-proof, water erosion and enhancing soil fertility etc.
Effect.The highest stage that mosses skinning is developed as biological breadcrust, the ability of fix the sand, increase soil stability and fertility
What is showed in various skinning is most strong.Attention recently as people to Arid&semi-arid area ecological environment, mosses skinning
It is artificial culture and its be concerned in soil sandification prevention and control and soil improvement etc..Currently, due to arid and semi-arid
The limitation of area's moisture condition, causes in the field environment, although mosses skinning has very strong drought-resistant ability, development slow
Slowly.In terms of existing research and technology are also concentrated mainly on artificial quick cultivation mosses skinning, about artificial culture mosses skinning
Drought-resistant ability is also seldom related to.The drought-resistant ability of artificial culture mosses skinning limits its implementation and application in the wild.How
Indoors condition can be turned out by way of manually cultivating cover degree and density it is higher and with naturally growth mosses knot
The same mosses skinning that can fix sandy soil surface of skin.The implementation for reinforcing this kind of field with drought-resistant ability mosses skinning, is to change
One of the main outlet of kind arid and semi-arid area ecological environment.
Invention content
Weak for anti-adversity ability in existing artificial culture mosses skinning in the wild harsh habitat, survival ability is poor, Wu Fashi
The reality that the dramatic shift in habitat should be coerced from laboratory condition with favourable conditions to field is to solve water stress
The most important problem that arid and semi-arid area moss generally faces is handgrip, and the purpose of the present invention is to provide a kind of artificial
The method for cultivating mosses skinning drought-resistant ability.
The purpose of the present invention is be achieved through the following technical solutions：
A method of husky area artificial culture mosses skinning field drought-resistant ability is improved, well-developed moss is selected
(Bryum argenteumHedw.), the cycle drought hardening for carrying out control water-arid-rehydration-recovery, i.e., first to manually training
The well-off moisture of moss (100% saturation) is educated, the moisture of 60% saturated water amount is imposed again after skinning surfaces exsiccation, makes
Skinning restore normal growth, and applies water again after skinning surfaces exsiccation, is the 20% of saturated water amount for confluent, waits for skinning surfaces
It is a cycle after being dried again, the Osmotic Adjustment Substances improved in moss body (make artificial culture mosses skinning
Osmotic Adjustment Substances reach 50% or more of field skinning), to improve its drought-resistant ability.
The cycle drought hardening of control water-arid-rehydration-recovery in above-mentioned steps 1 includes gaseous state water process and liquid water
Handle two methods：
A. gaseous state method for treating water is：Sample is placed in the translucent cover of humidifier and (humidifies 250 ml/h of power, adds
Wet 500 cm of skinning area2), 60 min are humidified first, humidify 30 min again after skinning surfaces exsiccation, skinning surfaces become again
10 min are humidified after dry.Will humidify 60 min after skinning surfaces be dried needed for time be set as applying the water period.From humidifying 60 min
Start, to humidification 10 min after skinning exsiccation be considered as an exercise period.It is normally cultivated when no humidification process.Drought resisting is taken exercise
At least continue 3 exercise periods；
B. liquid method for treating water is：It first waters artificial culture mosses to 100% saturation, after skinning surfaces exsiccation
The saturated water amount for imposing 60% again imposes 20% saturated water amount after skinning surfaces are dried again.Skinning after saturated water amount will be bestowed
Time needed for the exsiccation of surface is set as applying the water period.Since bestowing 100% saturated water amount, tied to after bestowing 20% saturated water amount
Skin exsiccation is considered as an exercise period.Drought resisting exercise at least continues 3 exercise periods.
During drought resisting described in above-mentioned steps 1 is taken exercise, condition of culture is temperature, air humidity, gas concentration lwevel, light
Period is fixed to 25/17 DEG C (day night), 60%, 400 ppm, 12 h.
Advantages of the present invention and the advantageous effect of generation are：
Present invention determine that a kind of best mode of artificial culture mosses skinning drought-resistant ability.It is well known that chlorophyll contains
Amount can reflect that the biomass and photosynthetic capacity of moss crust, malonaldehyde and soluble sugar content reflect the anti-of mosses skinning indirectly
Non-irrigated ability, cover degree can directly reflect the upgrowth situation of mosses skinning.It is subjected to different exercising ways and different exercises by measuring
Chlorophyll, malonaldehyde and the soluble sugar content and artificial culture mosses skinning of the artificial culture mosses skinning of number are out of office
The cover degree that skinning is formed under external environment improves mosses skinning drought-resistant ability moisture environment by artificial measures, preferably goes out artificial training
Educate the best drought resisting exercise method of mosses skinning.
The present invention has carried out drought resisting exercise to the mosses skinning of artificial culture, artificially gives plant with sublethal dose
Drought condition makes plant be subjected to the drought hardening of certain time, adapts to adverse circumstance, improves its drought-resistant ability, and forge by drought resisting
Refining starts the resistance mechanism of artificial culture moss itself, to make its Heredity of drought resistance characteristic under certain environmental conditions induction
It is showed.
The present invention is easy to operate, at low cost, time-consuming short, and artificial culture mosses skinning is drought-resistant by cycle drought hardening
Ability is notable, can preferably be promoted in production application.
Description of the drawings
Fig. 1 is influence of the liquid drought resisting exercising way to artificial culture mosses skinning chlorophyll content.
Fig. 2 is shadow of the gaseous state drought resisting exercising way to artificial culture mosses skinning chlorophyll content.
Fig. 3 is shadow of the liquid drought resisting exercising way to artificial culture mosses skinning soluble sugar content.
Fig. 4 is shadow of the gaseous state drought resisting exercising way to artificial culture mosses skinning soluble sugar content.
Fig. 5 is influence of the liquid drought resisting exercising way to artificial culture mosses skinning MDA contents.
Fig. 6 is shadow of the liquid drought resisting exercising way to artificial culture mosses skinning MDA contents.
Fig. 7 is influence (first 30 day daily apply water) of the liquid drought resisting exercising way to mosses bio-crust coverage.
Fig. 8 is influence (first 30 day daily apply water) of the gaseous state drought resisting exercising way to mosses bio-crust coverage.
Fig. 9 is influence (first 30 day every 3 day apply water) of the liquid drought resisting exercising way to mosses bio-crust coverage.
Figure 10 is influence (first 30 day every 3 day apply water) of the gaseous state drought resisting exercising way to mosses bio-crust coverage.
Specific implementation mode
In the following, the present invention is in conjunction with specific embodiments described further technical solution again：
A method of husky area artificial culture mosses skinning field drought-resistant ability is improved, well-developed moss is selected
(Bryum argenteumHedw.), the cycle drought hardening for carrying out control water-arid-rehydration-recovery, i.e., first to manually training
The well-off moisture of moss (100% saturation) is educated, the moisture of 60% saturated water amount is imposed again after skinning surfaces exsiccation, makes
Skinning restore normal growth, and applies water again after skinning surfaces exsiccation, is the 20% of saturated water amount for confluent, waits for skinning surfaces
It is a cycle after being dried again.During drought resisting is taken exercise, condition of culture is temperature, air humidity, gas concentration lwevel, light week
Phase is fixed to 25/17 DEG C (day night), 60%, 400 ppm, 12 h.
The cycle drought hardening of artificial culture mosses control water-arid-rehydration-recovery includes at gaseous state water process and liquid water
Manage two methods：
1. the determination of drought resisting exercise method：
1.1 gaseous state water process
(1) control group (A)：60 min of humidification daily, remaining time normally cultivate (humidification 250 ml/h of power, humidification knot
Surface accumulates 500 cm2, similarly hereinafter)；
(2) enhance drought hardening group (B) step by step：Sample is placed in the translucent cover of humidifier, the 1st day humidification 60
Min, the 2nd day 30 min of humidification, humidifies 10 min on the 3rd day, remaining time normally cultivates；(3) it is interrupted drought hardening processing group
(C1)：Sample is placed in the translucent cover of humidifier, humidifies 10 min daily, remaining time normally cultivates；
(4) interruption drought hardening processing group (C2)：Sample is placed in the translucent cover of humidifier, humidifies 30 daily
Min, remaining time normally cultivate；
1.2 liquid water process
The true moss skinning of artificial culture is acquired with the vessel of bottom hole, average thickness is about 10 mm.Experiment material will be housed
The vessel of material are placed in big pallet, and different water supplies is realized for confluent in big pallet by controlling.Test specimen
Share 4 processing groups：
(1) control group (A)：Every 3 days timing make-up water, every time 5 mm (saturated water amount)；
(2) enhance drought hardening group (B) step by step：1st day 5 mm of watering, pour 3 mm (60% saturated water amount) on the 4th day, and the 7th
It pours (20% saturated water amount) 1 mm；
(3) interruption drought hardening processing group (C1)：Watering in every 3 days is primary, every time 1 mm；
(4) interruption drought hardening processing group (C2)：Watering in every 3 days is primary, every time 3 mm；
After vaporous water and the equal duration exercises of liquid water process 1,3,5 time, by a part of sample of each group after drought hardening
Spontaneously dry, measure physical signs (the result is shown in Figure 1 ~ 3), another part sample rub it is broken after be seeded in field and prick the straw checkerboard barrier that has set
Interior (sample area:Straw checkerboard barrier area ≈ 1:10), 30 days of beginning regular make-up water are divided to (two kinds of processing, one group of daily make-up water
Point, every 3 days supply moisture of another set), prosthetic moisture applies after 30 days, and observation forms the cover degree variation (knot of mosses skinning
Fruit sees Fig. 4~5).
The drought-resistant ability for helping to improve artificial culture mosses skinning is tempered in the drought resisting of appropriateness, and excessive drought resisting is taken exercise may
Damage moss.The experimental results showed that the influence that gaseous state water process and liquid water process grow artificial culture mosses skinning
Identical (therefore below in relation to do not repartition vaporous water and liquid water process in the description of result), after 3 drought resistings exercise：
Each group chlorophyll content is：B groups>A groups>C2 groups>The chlorophyll content of C1 groups (Fig. 1), B groups is higher than other groups,
Illustrate that B group artificial culture mosses skinning growing ways are preferable.
Each group soluble sugar content size is：B groups>C2 groups>A groups>C1 groups (Fig. 2), artificial culture mosses after taking exercise 5 times
The soluble sugar content of skinning can reach field 4~August of true moss skinning soluble sugar content mean value (0.0422 umolcm-2)
60% or more, illustrate that the drought resisting using this method is taken exercise and improve the drought-resistant ability of artificial culture mosses skinning.And B groups can
Dissolubility sugared content is higher than other groups, illustrates that the drought resistance for the artificial culture mosses skinning taken exercise by drought resisting step by step is stronger, step by step
The effect for enhancing drought resisting exercise method is best.
Each group MDA content sizes are：B groups<C2 groups<A groups<C1 groups (Fig. 3), mosses skinning MDA contains under drought stress
Amount is stepped up with the intensification of Water deficit levels, and stress is more serious, and MDA contents are bigger.B group MDA contents are less than other groups,
Illustrate that the damage that drought resisting step by step is taken exercise to artificial culture mosses skinning is minimum.
Field sow the result shows that, apply under conditions of water (Fig. 4) daily within first 30 days, cover degree is：A groups>B groups>C2 groups>C1
Group, cover degree is after stopping moisture supply：B groups>C2 groups>C1 groups>A groups.Illustrate under the environment of moisture abundance, by drought resisting
The artificial culture moss growth of exercise may be not so good as unannealed moss, but the moss taken exercise by drought resisting
Drought resistance is high in the field environment, and survival ability is strong, wherein the effect that enhancing drought resisting is taken exercise step by step is best.When there are certain water
Divide stress, i.e., applies under conditions of water (Fig. 5) within every 3 days preceding 30 days, cover degree is：B groups>A groups>C2 groups>C1 groups stop moisture and supply
Cover degree is after answering：B groups>C1 groups>C2 groups>A groups illustrate to exist by the artificial culture mosses skinning that enhancing drought resisting is taken exercise step by step
Drought resistance is high under wild environment, and viability is stronger, and effect is best.
In summary as a result, with better drought resisting train on, vaporous water and liquid water process are equal for drought resisting exercise step by step
It can achieve the purpose that drought resisting is taken exercise.
Claims (3)
1. a kind of method improving artificial culture mosses skinning drought-resistant ability, it is characterised in that：Well-developed mosses are selected to plant
ObjectBryum argenteumHedw., the cycle drought hardening for carrying out control water-arid-rehydration-recovery, i.e., first to artificial culture
The well-off moisture of moss, moss reach saturation, impose the water of 60% saturated water amount again after skinning surfaces exsiccation
Point, so that skinning is restore normal growth, applies water again after skinning surfaces exsiccation, be the 20% of saturated water amount for confluent, wait for skinning
Surface is a cycle after being dried again, and the Osmotic Adjustment Substances of artificial culture mosses skinning is made to reach field skinning
50%, improve its drought-resistant ability.
2. according to a kind of method of raising artificial culture mosses skinning drought-resistant ability described in claim 1, it is characterised in that：
The cycle for controlling water-arid-rehydration-recovery includes two methods of gaseous state water process and liquid water process：
A. gaseous state method for treating water is：Sample is placed in the translucent cover of humidifier, 250 ml/h of power, humidification knot are humidified
Surface accumulates 500 cm2, mosses humidification skinning is humidified into 60 min first, 30 min, skinning are humidified again after skinning surfaces exsiccation
Surface humidifies 10 min after being dried again, will humidify 60 min after mosses skinning surfaces be dried needed for time be set as Shi Shuizhou
Phase, since humidifying 60 min, to humidification 10 min after mosses skinning exsiccation be considered as an exercise period；Without humidification process when
Normal culture is waited, drought resisting exercise at least continues 3 exercise periods；
B. liquid method for treating water is：It first waters artificial culture mosses to 100% saturation, waits for that mosses skinning skinning surfaces are dried
The saturated water amount for imposing 60% again afterwards imposes 20% saturated water amount, by mosses skinning after mosses skinning skinning surfaces are dried again
Bestow skinning surfaces after saturated water amount be dried needed for time be set as applying the water period, since bestowing 100% saturated water amount, to applying
Skinning exsiccation is considered as an exercise period after giving 20% saturated water amount, and drought resisting exercise at least continues 3 exercise periods.
3. a kind of method of raising artificial culture mosses skinning drought-resistant ability according to claims 1 or 2, it is characterized in that
Mosses skinning drought resisting take exercise during regular culture conditions be：25 DEG C of temperature daytime, 17 DEG C of night, air humidity 60%, dioxy
Change 400 ppm of concentration of carbon, the photoperiod is fixed to 12 h.
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| CN102204434B (en) * | 2011-03-25 | 2013-04-17 | 北京市农林科学院 | Method for preventing water erosion desertification by using biological crust |
| RU2500746C1 (en) * | 2012-12-21 | 2013-12-10 | Юрий Васильевич Захаров | Mulching composition (versions) |
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Effective date of registration: 20200806 Address after: 730000 No.318, Donggang West Road, Chengguan District, Lanzhou City, Gansu Province Patentee after: NORTHWEST INSTITUTE OF ECO-ENVIRONMENT AND RESOURCES, CAS Address before: Chengguan District of Gansu city of Lanzhou province Donggang West Road 730000 No. 320 Patentee before: Institute of environment and Engineering in cold and dry areas, Chinese Academy of Sciences |
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