CN106688985A - Artificial egg collecting and hatching method of California holothurian - Google Patents

Artificial egg collecting and hatching method of California holothurian Download PDF

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Publication number
CN106688985A
CN106688985A CN201710183834.4A CN201710183834A CN106688985A CN 106688985 A CN106688985 A CN 106688985A CN 201710183834 A CN201710183834 A CN 201710183834A CN 106688985 A CN106688985 A CN 106688985A
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CN
China
Prior art keywords
eggs
ovum
holothurian
hatching
california
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710183834.4A
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Chinese (zh)
Inventor
王文琪
徐申波
梁彩凤
王鑫
任贻超
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qingdao Agricultural University
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Qingdao Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qingdao Agricultural University filed Critical Qingdao Agricultural University
Priority to CN201710183834.4A priority Critical patent/CN106688985A/en
Publication of CN106688985A publication Critical patent/CN106688985A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention discloses an artificial egg collecting and hatching method of California holothurian. The artificial egg collecting and hatching method of the California holothurian includes steps: (1) collecting eggs: performing egg collecting of parent holothurian through an artificial egg collecting insemination method, temperature rising or reducing induction and a shady drying running water comprehensive stimulation method; (2) washing the eggs: timely moving the parent holothurian out after the parent holothurian lays the eggs and the eggs are inseminated, and washing the eggs by draining and adding clean seawater more than once (siphoning out the seawater through a 260 mesh net cage); after concentrating the eggs with a net cage made of 300 mesh bolting silk, brushing the eggs more than once by using the clear seawater; (3) hatching. The artificial egg collecting and hatching method of the California holothurian first collects the eggs and washes the eggs after being collected with the clear seawater, and meets demands of the holothurian eggs for hatching environment, facilitates rapid development of inseminated eggs, and improves survival rate during the hatching process after the eggs are washed.

Description

A kind of ovum artificial collection and hatching method of California sea cucumber
Technical field
Ovum technical field is adopted the present invention relates to sea cucumber, the artificial of specifically a kind of California sea cucumber is adopted Ovum and hatching method.
Background technology
Sea cucumber originates in North America in California, is Important Economic species of the California to Alaska intertidal zone, Nutritious because its meat is tender, individual big, body wall thorniness, meat is thick, in good taste, for many years by domestic and international consumer's Favor.Different regions occur in that the upsurge of cultivation California sea cucumber in succession in recent years.Therefore sea cucumber adopt ovum and hatching skill Art is particularly important.
The content of the invention
Defect and deficiency it is an object of the invention to be directed to prior art, there is provided a kind of people of California sea cucumber Work adopts ovum and hatching method.
To achieve the above object, the technical solution adopted by the present invention is:
A kind of ovum artificial collection and hatching method of California sea cucumber, its step is:
1st, ovum is adopted:Carried out by the method for ovum artificial collection fertilization method, intensification or cooling induction and water integrated stimulation of drying in the shade Ovum is adopted in parent participation;
2nd, ovum is washed:Parent participation is removed in time after parent participation spawning and fertilization, with clean seawater by draining, adding water is carried out for several times Wash ovum (the 260 outside siphons of mesh net cage of draining);After the net cage being made of 300 mesh sieve thin,tough silk concentrates ovum, washed with clean seawater Brush is for several times;
3rd, hatch:
1. environmental condition:Salinity is 25-30 ‰;18-22 DEG C of water temperature, general control is at 20---21 DEG C;Illumination 500- 1000lx;PH7.8—8.3;Continuous micro is inflated or every 0.5 hour stirs water once;
2. density:By 1-5 m/l incubating oosperms;
3. incubator:Ovum is produced in spawning case, after washing ovum, has been carried out in former case by the density of 0.4---0.5/ml Hatching.
Beneficial effects of the present invention are:The present invention is first passed through and adopts ovum, to be adopted and wash ovum using clean seawater after ovum, wash after ovum The process hatched, meets the demand of the environment of hatching of sea cucumber children's ovum, is easy to embryonated egg Rapid development, improves survival rate.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, it is right below in conjunction with specific embodiment The present invention is further elaborated.It should be appreciated that specific embodiment described herein is only used to explain the present invention, and It is not used in the restriction present invention.
Embodiment:
A kind of ovum artificial collection and hatching method of California sea cucumber, its step is:
1st, ovum is adopted:Carried out by the method for ovum artificial collection fertilization method, intensification or cooling induction and water integrated stimulation of drying in the shade Ovum is adopted in parent participation;
2nd, ovum is washed:Parent participation is removed in time after parent participation spawning and fertilization, with clean seawater by draining, adding water is carried out for several times Wash ovum (the 260 outside siphons of mesh net cage of draining);After the net cage being made of 300 mesh sieve thin,tough silk concentrates ovum, washed with clean seawater Brush is for several times;
3rd, hatch:
1. environmental condition:Salinity 30 ‰;22 DEG C of water temperature, general control is at 21 DEG C;Illumination 1000lx;PH8.3;Continuous micro Inflation or every 0.5 hour agitation water are once;
2. density:By 5 m/l incubating oosperms;
3. incubator:Ovum is produced in spawning case, after washing ovum, has been hatched in former case by the density of 0.5/ml.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be in other specific forms realized.Therefore, no matter From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power Profit requires to be limited rather than described above, it is intended that all in the implication and scope of the equivalency of claim by falling Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each implementation method is only wrapped Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should Specification an as entirety, the technical scheme in each embodiment can also be formed into those skilled in the art through appropriately combined May be appreciated other embodiment.

Claims (1)

1. a kind of ovum artificial collection and hatching method of California sea cucumber, it is characterised in that:Step is:
(1) ovum, is adopted:Parent is carried out by the method for ovum artificial collection fertilization method, intensification or cooling induction and water integrated stimulation of drying in the shade That joins adopts ovum;
(2) ovum, is washed:Parent participation is removed in time after parent participation spawning and fertilization, with clean seawater by draining, adding water washed for several times Ovum (draining with the 260 outside siphons of mesh net cage);After the net cage being made of 300 mesh sieve thin,tough silk concentrates ovum, scrubbed with clean seawater For several times;
(3), hatch:
1. environmental condition:Salinity is 25-30 ‰;18-22 DEG C of water temperature, general control is at 20---21 DEG C;Illumination 500- 1000lx;PH7.8—8.3;Continuous micro is inflated or every 0.5 hour stirs water once;
2. density:By 1-5 m/l incubating oosperms;
3. incubator:Ovum is produced in spawning case, after washing ovum, has been incubated in former case by the density of 0.4---0.5/ml Change.
CN201710183834.4A 2017-03-24 2017-03-24 Artificial egg collecting and hatching method of California holothurian Pending CN106688985A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710183834.4A CN106688985A (en) 2017-03-24 2017-03-24 Artificial egg collecting and hatching method of California holothurian

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710183834.4A CN106688985A (en) 2017-03-24 2017-03-24 Artificial egg collecting and hatching method of California holothurian

Publications (1)

Publication Number Publication Date
CN106688985A true CN106688985A (en) 2017-05-24

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710183834.4A Pending CN106688985A (en) 2017-03-24 2017-03-24 Artificial egg collecting and hatching method of California holothurian

Country Status (1)

Country Link
CN (1) CN106688985A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115413612A (en) * 2022-09-15 2022-12-02 中国科学院南海海洋研究所 Non-irritant gathering and spawning method for holothuria leucospilota

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102100206A (en) * 2011-01-04 2011-06-22 陈水春 Artificial breeding method for tropical sea cucumbers
CN103026992A (en) * 2012-11-29 2013-04-10 大连棒棰岛海参发展有限公司 Stichopus japonicus seedling large-scale ecological culture method utilizing outdoor net cages
CN103355224A (en) * 2012-03-27 2013-10-23 响水海辰农业发展有限公司 Novel stichopus japonicus fry breeding method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102100206A (en) * 2011-01-04 2011-06-22 陈水春 Artificial breeding method for tropical sea cucumbers
CN103355224A (en) * 2012-03-27 2013-10-23 响水海辰农业发展有限公司 Novel stichopus japonicus fry breeding method
CN103026992A (en) * 2012-11-29 2013-04-10 大连棒棰岛海参发展有限公司 Stichopus japonicus seedling large-scale ecological culture method utilizing outdoor net cages

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
夏斌等: "加利福尼亚红刺参胚胎及幼体发育过程", 《水产学报》 *
王春生等: "《无公害刺参、鲍养殖技术》", 30 June 2005, 山东科学技术出版社 *
科学技术部中国农村技术开发中心组: "《设施农业在中国》", 31 October 2006, 中国农业科学技术出版社 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115413612A (en) * 2022-09-15 2022-12-02 中国科学院南海海洋研究所 Non-irritant gathering and spawning method for holothuria leucospilota

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