CN106688554A - Method for selecting organic-selenium-rich wheat variety - Google Patents
Method for selecting organic-selenium-rich wheat variety Download PDFInfo
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- CN106688554A CN106688554A CN201611112650.0A CN201611112650A CN106688554A CN 106688554 A CN106688554 A CN 106688554A CN 201611112650 A CN201611112650 A CN 201611112650A CN 106688554 A CN106688554 A CN 106688554A
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Abstract
The invention relates to a method for selecting an organic-selenium-rich wheat variety and belongs to the field of crop production. The method comprises the steps that to-be-detected wheat varieties are sown on test land comprising a test area, wherein the selenium content of the soil in the test land is less than or equal to 0.2 mg/kg; intervention is conducted on the test area by means of a selenium-rich nutritional agent; after the wheat is harvested, the total selenium content and the organic selenium content of each to-be-detected wheat variety are detected, and when the total selenium content of the wheat is 0.2-0.5 mg/kg and the organic selenium content of the wheat is larger than or equal to 80%, the wheat variety is determined as the organic-selenium-rich wheat variety. By means of the method, the wheat variety which is high in selenium-rich capacity can be selected, and the method is beneficial to the variety selection of selenium-rich wheat production.
Description
Technical field
The present invention relates to field of crop production, and more particularly to a kind of screening technique of Wheat rich in organic selenium kind.
Background technology
Selenium is a kind of chemical element, chemical symbol Se, nutrient and beneficial to plant necessary to be a kind of animal body
Nutrient.Selenium element is trace quantity mineral substance element needed by human, and human body selenium total content in itself is 6mg-20mg.Due to human body
The interior organ in the absence of storage selenium, the selenium needed for body constantly should feed from diet.
Wheat is one of staple food that daily people can usually eat, and selenium element necessary to human body can be from edible wheat
Middle acquisition, but Se-rich wheat on the market is only the addition and addition that selenium element is carried out in the planting process of wheat now
Mode is unreasonable, meanwhile, to wheat addition be only in the prior art inorganic selenium, inorganic selenium can to human body toxic side effect, and
And also without the per mu yield situation for sufficiently considering wheat, furthermore, do not have the systematic selenium rich ability to wheat to be estimated, do not have yet
There is the wheat breed for clearly selecting selenium-rich, so the also blindness with Fruit variety in the production of Se-rich wheat, no
The economic worth of Se-rich wheat, nutritive value can to greatest extent be brought into play.
The content of the invention
It is an object of the invention to provide a kind of screening technique of Wheat rich in organic selenium kind, it can be by Selenium-rich nutrient
Wheat is intervened, the absorbability of the wheat to selenium element of different cultivars is determined, and the wheat of different cultivars selenium-rich energy
Power, so as to the wheat breed with stronger organic selenium-rich ability selected, optimizes the production of Se-rich wheat, and lifting selenium-rich is small
The nutrition of wheat and economic worth.
The present invention is solved its technical problem and is realized using following technical scheme.
A kind of screening technique of Wheat rich in organic selenium kind, including：Selection soil selenium-containing amount is less than or equal to 0.2mg/kg's
Experiment soil, experiment soil includes trial zone, and kind wheat to be detected is sowed in trial zone, and trial zone is carried out with Selenium-rich nutrient
Selenium-rich is intervened, and total selenium content and organic selenium content of each kind wheat to be detected of results are detected after results, when trial zone is received
The total selenium content of wheat for obtaining be 0.2mg/kg-0.5mg/kg, and Organic Selenium content be more than or equal to 80% when, judge it is to be detected
Kind wheat is Wheat rich in organic selenium kind.
The beneficial effect of the screening technique of the Wheat rich in organic selenium kind of the embodiment of the present invention is：Can be by of the invention
The screening technique of Wheat rich in organic selenium kind determines absorption accumulation ability of each wheat breed to selenium element, so as to filter out energy
The wheat breed of enough abundant organic selenium-rich, is optimized the plant development of follow-up Se-rich wheat.
Specific embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below will be in the embodiment of the present invention
Technical scheme be clearly and completely described.Unreceipted actual conditions person, builds according to normal condition or manufacturer in embodiment
The condition of view is carried out.Agents useful for same or the unreceipted production firm person of instrument, are the conventional product that can be obtained by commercially available purchase
Product.
The screening technique to the Wheat rich in organic selenium kind of the embodiment of the present invention is specifically described below.
A kind of screening technique of Wheat rich in organic selenium kind that the present invention is provided, random plantation kind wheat to be detected,
And intervened with Selenium-rich nutrient in the trial zone for carrying out wheat selenium-rich experiment, carry out total selenium content after results and Organic Selenium contains
The detection of amount, when wheat total selenium content be 0.2mg/kg-0.5mg/kg, and Organic Selenium content be more than or equal to 80% when,
The wheat of the kind is Wheat rich in organic selenium kind.
Specifically, the screening of Se-rich wheat kind, is carried out according to following methods：
In embodiments of the invention, the soil first to Wheat rich in organic selenium screening varieties to be carried out is selected, selection
Standard is uniform soil fertility, and Se content is less than or equal to 0.2mg/kg, and can be easy to the field irrigated.The soil in selected soil
Loamy texture amount should comply with《GB 15618-1995》Requirement, and the standard of irrigation water should meet《GB 22573-2008》
With《GB 22574-2008》Standard.
The experiment soil selected can be divided into trial zone and blank district, and trial zone will be used into selenium-rich nutritive
Agent carries out selenium-rich intervention, and screens the kind of Se-rich wheat in trial zone；Blank district does not carry out selenium-rich intervention, keeps its low
The state of selenium；The per mu yield of each wheat under the planting environment of different selenium contents will be carried out between trial zone and blank district
Amount compares, to judge kind wheat to be detected while the enrichment of selenium element is carried out, if can have good growing way and product
Amount.Kind wheat to be detected is sowed in trial zone and blank district.It should be further stated that, in trial zone and blank
The surrounding experimental field of the screening Se-rich wheat kind that check plot is formed, is provided with the protection row of 1m-1.2m wide, in protection row
A kind of random wheat selected from the wheat breed to be screened of plantation.And protect row and experimental field between be also installed with width
The aisle of 0.4m-0.6m, is easy to staff's walking about when field is managed and works.
Above-mentioned selenium-rich intervention includes that plantation is preceding and intervenes and intervene after planting.It is in kind wheat to be detected to intervene before plantation
Before being seeded in trial zone, the mineral fertilizer containing selenium is applied into trial zone；Intervention is the heading in kind wheat to be detected after plantation
Blooming stage sprays in the blade face of kind wheat to be detected organic selenium-rich liquid fertilizer.
The administration standard of the above-mentioned mineral fertilizer containing selenium is 100kg/ mus -120kg/ mus, and Se content therein is more than or equal to
200mg/kg；The application concentration of organic selenium-rich liquid fertilizer is 30mg/L, and above-mentioned application concentration is every liter after dilute with water to be rich in
Selenium content in machine selenium liquid fertilizer is 30mg, while the absolute Se content of every mu of organic selenium-rich liquid fertilizer of ground administration is 1g-
1.5g, preferably, the absolute Se content of every mu of organic selenium-rich liquid fertilizer of ground administration is 1.2g, the wheat for both having met is to selenium unit
The demand of element, turn avoid unnecessary waste.
The organic selenium-rich liquid fertilizer is that culture forms test tube in being inoculated into test tube rejuvenation culture medium by bacillus subtilis
Kind, then test tube kind is inoculated into fermentation tank and inorganic selenium is carried out fermentation and be obtained, wherein, the composition bag of test tube rejuvenation culture medium
Include：6g/L glucose, 5g/L sucrose, 5g/L beef extracts, 5g/L peptones, 4g/L sodium chloride, 18g/L agar；Bag in fermentation tank
Include：6g/L glucose, 5g/L sucrose, 5g/L beef extracts, 10g/L peptones, 4g/L sodium chloride, biofermentation defoamer 1g/L.
The ratio of the area of trial zone and the area of blank district can be 2:1, that is, ensure that trial zone can be set
Repeat to test, it is ensured that the accuracy of experiment, tested number is reduced again, reduce certain experimentation cost.But above-mentioned trial zone
It is not unique with the setting of the area ratio of blank district, the area ratio of trial zone and blank district can also be 1:
1、3:1 etc..
It is possible to further trial zone is divided into multiple experimental cultivation areas, the area in multiple experimental cultivation areas is phase
Deng, for example can be 6.7m2-15m2.Trial zone includes the first trial zone and second experiment in equal number experimental cultivation area
Area., each kind wheat to be detected respectively at least one experimental cultivation area of the first trial zone and the second trial zone extremely
Few experimental cultivation area plantation, you can carry out random district's groups plantation respectively with above-mentioned the first trial zone and the second trial zone
Experiment.
Blank district is divided into multiple blank growing areas according to the division methods of trial zone, multiple blank growing areas
Area is equal, and area all with experimental cultivation area is identical.By each kind wheat planting to be detected at least one blank
In growing area, i.e., the kind of each kind wheat is carried out according to random district's groups implantation methods in the blank growing area of blank district
Plant, the random plantation of wheat of a kind is selected at random in any one blank growing area of blank district.
It should be noted that the quantity in experimental cultivation area in the first trial zone, the quantity in the second experimental cultivation area and
The quantity of blank growing area is determined according to the wheat breed quantity to be carried out Wheat rich in organic selenium screening varieties, example
Such as：Carrying out the wheat of Se-rich wheat Fruit variety has 24 kinds, experimental cultivation area and the second experiment in the first trial zone
The experimental cultivation area in area and the quantity of blank growing area are 24, it is ensured that the wheat of each kind has corresponding two experiments
Plantation and a blank plantation；If the kind of the wheat of selenium-rich screening is carried out for 16 kinds, 20 kinds etc., to experimental cultivation area
Quantity with blank growing area is adjusted correspondingly.It should be further stated that, between adjacent experimental cultivation area, phase
Spaced, the width at above-mentioned interval between adjacent blank growing area and between adjacent experimental cultivation area and blank growing area
It is 0.4m-0.6m to spend, and the setting at interval can avoid the variet complexity in experiment, can also be easy to staff in the pipe in field
Reason operation.
Further, the standard of the application rate of wheat seed broadcasts 13kg-15kg for mu.
The process of plantation and management when the screening of Se-rich wheat kind is carried out, according to conventional High-Yield Cultivation of Wheat skill
Art is carried out, and Term Fertilization standard in Cultivate administration will meet《NY/T 394》Regulation.Specifically mode is as follows：
Arable land：Deep ploughed in trial zone and blank district respectively, cultivated depth is 25cm-30cm, then basal dressing
With the above-mentioned mineral fertilizer containing selenium, fine site preparation ploughs flat.Above-mentioned base manure is farm manure, and the standard of fertilising is every mu to apply
1000kg-2000kg。
Seed Treatment：The seed of the Wheat rich in organic selenium kind with bactericide to filtering out carries out sterilized seed dressing, is killed
Strain, above-mentioned bactericide includes olefin conversion and carbendazim, it is not limited to this；And the consumption dressed seed with bactericide
It is the 0.2% of seed weight.
Sowing：The depth of planting of wheat seed is 4cm-5cm, and line-spacing is 20cm-25cm, and the application rate on every mu of ground is
10kg-15kg.The time of sowing can determine according to the geographical position where the wheatland of selection and the climatic condition in the field.
For example：In very cold, it is adapted to the area of plant spring wheat, the sowing of wheat can be carried out in March-April；And in weather temperature
Warm area, then can be with 11 months-December Winter Wheat Planted.
Fertilising：Above-mentioned organic selenium-rich liquid fertilizer is sprayed in the heading flowering period of wheat, and two are carried out in the growth period of wheat
Secondary fertilising, is for the first time the period of seedling establishment in wheat, and every mu of ground is more than or equal to using the urea of 20kg-25kg or the content of nitrogen
40% formula fertilizer；It it is for the second time the boot stage in wheat, the urea of 7kg-8kg is applied on every mu of ground.
Weeding and wheatland control：Before wheat jointing, its drug resistance is most strong, can only wait chemical herbicide to be sprayed with wheat straw
Apply, weeds are removed；In order to prevent wheat excessive growth and later stage from lodging, chemical control agent the carrying out control such as paclobutrazol can be used；But it is chemical
Weeding and Chemical Control lodging-prevention are intended to be stopped using after the jointing stage of wheat, and poisoning, influence will be otherwise produced to wheat
Wheat it is solid.
Harvesting：If the winter wheat of November-December plantation, then the 5-6 middle of the month in Second Year enters harvesting, and dries
Storage；If the spring wheat of March-April plantation, then enter harvesting in mid-July then.
After results, to the wheat of all kinds, the detection of Se content is carried out respectively according to kind difference, when always containing for wheat
Selenium amount is 0.2mg/kg-0.5mg/kg, and the content of Organic Selenium, when being more than or equal to 80%, the wheat of the kind is organic selenium-rich
Wheat breed, when total selenium content of wheat is less than 0.2mg/kg more than 0mg/kg, the wheat of the kind is wheat containing selenium.
Below in conjunction with the embodiment of screening technique of Wheat rich in organic selenium kind, the present invention is described in further detail.
Embodiment 1
November 1, one piece of soil selenium-containing amount of selection carries out Wheat rich in organic selenium screening varieties for the arable land of 0.2mg/kg
Experiment, the surrounding in the soil region to be tested sets the protection row of width 1m and is stayed between protection row and experiment soil
If aisle 0.4m wide, the wheat breed planted on protection row is the western wheat 0638-1 in Anhui.The soil of trial zone and blank district
Deep ploughed, tilling depth 25cm, and the land equalization is divided into the first trial zone, the second trial zone and blank district, with every mu
The standard of 1000kg is applied on ground, applies farm manure.The first trial zone and the second trial zone are given with amount of application 100kg/ mus of standard
Using selenium content for the mineral fertilizer containing selenium of 200mg/kg carries out planting preceding intervention, distinguish in the first trial zone and the second trial zone
24 areas are marked off for 6.7m2Experimental cultivation area, mark off 24 areas for 6.7m in blank district2Blank plantation
Area, the interval in adjacent experimental cultivation area and interval and adjacent experimental cultivation area and the blank kind of adjacent blank growing area
Growing area at intervals of 0.4m.
It is randomly assigned to plant 24 wheats of kind in 24 experimental cultivation areas of the first trial zone, similarly in the second examination
Test area and blank test area and plant the wheat of above-mentioned 24 kinds at random；The application rate standard of wheat seed broadcasts 13kg for mu, and
Prior to seeding sterilized seed dressing is carried out with the 0.2% of seed weight olefin conversion；The depth of wheat cultivation is 4cm, and line-spacing is 20cm.
24 above-mentioned wheats of kind be respectively the western wheat 0638-1 in Anhui, red No. 3-2 of state, poplar wheat 23-3, China 2152-6, Hubei Province wheat 18-7,
Southern agriculture 14Y106-8, letter wheat 8811-9, peaceful wheat 26-10, China 2566-11, raise wheat 20-12, Hubei Province wheat 580-13, Hubei Province wheat 23-15, assist
Wheat 25-16, China 1168-17, China 2668-18, assist wheat 55-19, Hubei Province grace No. 7-21, Zheng wheat 9023-23, Hubei Province wheat 251-24, E Mai
170-5, E-en No.6-14, Hubei Province wheat 596-20, assist wheat 35-22, Hubei Province wheat 352-4.
It is the organic selenium-rich liquid fertilizer of 30mg/L in the heading flowering period spraying concentration of wheat, wherein the richness that every mu of ground is sprayed
Se content in Organic Selenium liquid fertilizer is 1.2g；In the period of seedling establishment of wheat, mu applies the urea of 20kg；In the boot stage of wheat, mu
Apply the urea of 7kg.Before wheat jointing, sprayed only with wheat straw, weeds are removed；Prevent excessive growth and later stage from falling with paclobutrazol
Volt.Mid-May, gathered in, dried, and the wheat of the first trial zone and the second trial zone is carried out Se content detection and
And compare the per mu yield of the first trial zone, the second trial zone and each kind wheat of blank district.
Embodiment 2
November 5, one piece of soil selenium-containing amount of selection carries out Wheat rich in organic selenium screening varieties for the arable land of 0.15mg/kg
Experiment, the surrounding in the soil region to be tested sets the protection row of width 1.2m and between protection row and experiment soil
Stay and set 0.6m aisle wide, the wheat breed planted on protection row is red No. 3-2 of state.Depth is carried out to trial zone and blank district
Plough, tilling depth 30cm, and the land equalization is divided into trial zone and blank district, every mu to be applied using the standard of 2000kg
Farm manure.The mineral fertilizer containing selenium with amount of application 120kg/ mus of standard to trial zone using selenium content as 250mg/kg is planted
Intervene before planting, 20 areas are marked off for 15m in trial zone2Experimental cultivation area, mark off 20 areas in blank district
It is 15m2Blank growing area, interval and the adjacent examination at the interval and adjacent blank growing area in adjacent experimental cultivation area
Test growing area and blank growing area at intervals of 0.6m.
It is randomly assigned to plant 20 wheats of kind in 20 experimental cultivation areas of trial zone, similarly in blank test area
20 above-mentioned wheats of kind are planted at random；The application rate standard of wheat seed broadcasts 15kg for mu, and uses seed prior to seeding
The carbendazim of the 0.2% of weight carries out sterilized seed dressing；The depth of wheat cultivation is 5cm, and line-spacing is 25cm.20 above-mentioned kinds
Wheat be respectively the western wheat 0638-1 in Anhui, red No. 3-2 of state, poplar wheat 23-3, China 2152-6, Hubei Province wheat 18-7, Nan Nong 14Y106-8, letter
Wheat 8811-9, peaceful wheat 26-10, China 2566-11, raise wheat 20-12, Hubei Province wheat 580-13, Hubei Province wheat 23-15, assist wheat 25-16, China 1168-
17th, China 2668-18, assist wheat 55-19, Hubei Province grace No. 7-21, Zheng wheat 9023-23, Hubei Province wheat 251-24, Hubei Province wheat 170-5.
It is the organic selenium-rich liquid fertilizer of 30mg/L in the heading flowering period spraying concentration of wheat, wherein the richness that every mu of ground is sprayed
Se content in Organic Selenium liquid fertilizer is 1g；In the period of seedling establishment of wheat, mu applies the formula fertilizer that the content of the nitrogen of 25kg is equal to 40%；
In the boot stage of wheat, mu applies the urea of 8kg.Before wheat jointing, sprayed with Dicamba, weeds are removed；It is rich with strengthening
Security protection stops excessive growth and later stage lodging.Mid-May, gathered in, dried, and the wheat of trial zone is carried out Se content detection,
And comparative test area and the per mu yield of each kind wheat of blank district.
Embodiment 3
November 10, one piece of soil selenium-containing amount of selection carries out Wheat rich in organic selenium screening varieties for the arable land of 0.2mg/kg
Experiment, the surrounding in the soil region to be tested sets the protection row of width 1.1m and between protection row and experiment soil
Stay and set 0.5m aisle wide, the wheat breed planted on protection row is poplar wheat 23-3.The soil of trial zone and blank district enters
Row deep ploughing, tilling depth 27cm, and the land equalization is divided into four pieces, wherein three pieces is trial zone, it is blank district to be left one piece,
Every mu to apply the standard of 1500kg, farm manure is applied, selenium content is applied to trial zone with amount of application 110kg/ mus of standard
For the mineral fertilizer containing selenium of 240mg/kg carries out planting preceding intervention.Mark off three areas in trial zone, be respectively the first trial zone,
Contain 16 areas for 9m in second trial zone and the 3rd trial zone, above-mentioned each area2Experimental cultivation area, in blank zoning
16 areas are separated for 9m2Blank growing area, the interval and the interval of adjacent blank growing area in adjacent experimental cultivation area
And adjacent experimental cultivation area and blank growing area at intervals of 0.5m.
It is randomly assigned to plant 16 wheats of kind in 16 experimental cultivation areas of the first trial zone, similarly in the second examination
Test area, the 3rd trial zone and blank test area and plant 16 above-mentioned wheats of kind at random；The application rate standard of wheat seed
For mu broadcasts 14kg, and with the 0.2% of seed weight olefin conversion carry out sterilized seed dressing prior to seeding；The depth of wheat cultivation is
4.5cm, line-spacing is 22cm.16 above-mentioned wheats of kind are respectively the western wheat 0638-1 in Anhui, red No. 3-2 of state, poplar wheat 23-3, China
2152-6, Hubei Province wheat 18-7, Nan Nong 14Y106-8, letter wheat 8811-9, peaceful wheat 26-10, China 2566-11, raise wheat 20-12, E Mai
580-13, Hubei Province wheat 23-15, assist wheat 25-16, China 1168-17, China 2668-18, assist wheat 55-19.
It is the organic selenium-rich liquid fertilizer of 30mg/L in the heading flowering period spraying concentration of wheat, wherein the richness that every mu of ground is sprayed
Se content in Organic Selenium liquid fertilizer is 1.5g；In the period of seedling establishment of wheat, mu applies the formula that the content of the nitrogen of 22kg is equal to 50%
Fertilizer；In the boot stage of wheat, mu applies the urea of 7.5kg.Before wheat jointing, sprayed with Dicamba, weeds are removed；With
Zhuang Feng An prevents excessive growth and later stage from lodging.Mid-June, gathered in, dried, and the inspection of Se content is carried out to the wheat of trial zone
Survey and the per mu yield of comparative test area and each kind wheat of blank district.
Embodiment 4
November 15, one piece of soil selenium-containing amount of selection carries out Wheat rich in organic selenium screening varieties for the arable land of 0.17mg/kg
Experiment, the surrounding in the soil region to be tested set the protection row of width 1.1m and protection row and test soil it
Between stay and set 0.4m aisle wide, the wheat breed planted on protection row is the western wheat 0638-1 in Anhui.Trial zone and blank district
Soil is deep ploughed, tilling depth 26cm, and the land equalization is divided into the first trial zone, the second trial zone and blank district, with
The standard of 1800kg is applied on every mu of ground, applies farm manure.With amount of application 105kg/ mus of standard to the first trial zone and the second examination
Area is tested using selenium content for the mineral fertilizer containing selenium of 200mg/kg carries out planting preceding intervention, in the first trial zone and the second trial zone
24 areas are respectively divided out for 6.7m2Experimental cultivation area, mark off 24 areas for 6.7m in blank district2Blank
Growing area, the interval in adjacent experimental cultivation area and interval and adjacent experimental cultivation area and the sky of adjacent blank growing area
White race growing area at intervals of 0.5m.
It is randomly assigned to plant 24 wheats of kind in 24 experimental cultivation areas of the first trial zone, similarly in the second examination
Test area and blank test area and plant the wheat of above-mentioned 24 kinds at random；The application rate standard of wheat seed broadcasts 14kg for mu, and
Prior to seeding sterilized seed dressing is carried out with the 0.2% of seed weight how clever bacterium；The depth of wheat cultivation is 4.7cm, and line-spacing is
22cm.24 above-mentioned wheats of kind are respectively the western wheat 0638-1 in Anhui, red No. 3-2 of state, poplar wheat 23-3, China 2152-6, E Mai
18-7, Nan Nong 14Y106-8, letter wheat 8811-9, peaceful wheat 26-10, China 2566-11, raise wheat 20-12, Hubei Province wheat 580-13, Hubei Province wheat 23-
15th, assist wheat 25-16, China 1168-17, China 2668-18, assist wheat 55-19, Hubei Province grace No. 7-21, Zheng wheat 9023-23, Hubei Province wheat 251-24,
Hubei Province wheat 170-5, E-en No.6-14, Hubei Province wheat 596-20, assist wheat 35-22, Hubei Province wheat 352-4.
It is the organic selenium-rich liquid fertilizer of 30mg/L in the heading flowering period spraying concentration of wheat, wherein the richness that every mu of ground is sprayed
Se content in Organic Selenium liquid fertilizer is 1.4g；In the period of seedling establishment of wheat, mu applies the urea of 22kg；In the boot stage of wheat, mu
Apply the urea of 7kg.Before wheat jointing, sprayed only with wheat straw, weeds are removed；Prevent excessive growth and later stage from falling with paclobutrazol
Volt.Mid-June, gathered in, dried, and the wheat of the first trial zone and the second trial zone is carried out Se content detection and
And compare the per mu yield of the first trial zone, the second trial zone and each kind wheat of blank district.
Embodiment 5
November 20, one piece of soil selenium-containing amount of selection carries out Wheat rich in organic selenium screening varieties for the arable land of 0.2mg/kg
Experiment, the surrounding in the soil region to be tested sets the protection row of width 1.2m and between protection row and experiment soil
Stay and set 0.6m aisle wide, the wheat breed planted on protection row is red No. 3-2 of state.Depth is carried out to trial zone and blank district
Plough, tilling depth 28cm, and the land equalization is divided into trial zone and blank district, every mu to apply the standard of 1800kg, apply
Enter farm manure.The mineral fertilizer containing selenium with amount of application 110kg/ mus of standard to trial zone using selenium content as 220mg/kg is carried out
Intervene, 20 areas are marked off for 15m in trial zone2Experimental cultivation area, mark off 20 areas for 15m in blank district2
Blank growing area, the interval in adjacent experimental cultivation area and interval and the adjacent experimental cultivation of adjacent blank growing area
Area and blank growing area at intervals of 0.6m.
It is randomly assigned to plant 20 wheats of kind in 20 experimental cultivation areas of trial zone, similarly in blank test area
20 above-mentioned wheats of kind are planted at random；The application rate standard of wheat seed broadcasts 13.5kg, and use kind prior to seeding for mu
The carbendazim of the 0.2% of sub- weight carries out sterilized seed dressing；The depth of wheat cultivation is 4.2cm, and line-spacing is 21cm.Above-mentioned 20
The wheat of kind is respectively the western wheat 0638-1 in Anhui, red No. 3-2 of state, poplar wheat 23-3, China 2152-6, Hubei Province wheat 18-7, Nan Nong 14Y106-
8th, letter wheat 8811-9, peaceful wheat 26-10, China 2566-11, raise wheat 20-12, Hubei Province wheat 580-13, Hubei Province wheat 23-15, assist wheat 25-16, China
1168-17, China 2668-18, assist wheat 55-19, Hubei Province grace No. 7-21, Zheng wheat 9023-23, Hubei Province wheat 251-24, Hubei Province wheat 170-5.
It is the organic selenium-rich liquid fertilizer of 30mg/L in the heading flowering period spraying concentration of wheat, wherein the richness that every mu of ground is sprayed
Se content in Organic Selenium liquid fertilizer is 1.1g；In the period of seedling establishment of wheat, mu applies the formula that the content of the nitrogen of 20kg is equal to 40%
Fertilizer；In the boot stage of wheat, mu applies the urea of 8kg.Before wheat jointing, sprayed with Dicamba, weeds are removed；With strong
Feng An prevents excessive growth and later stage from lodging.At the beginning of 6 months, gathered in, dried, and the wheat of trial zone is carried out Se content detection,
And comparative test area and the per mu yield of each kind wheat of blank district.
Embodiment 6
November 25, one piece of soil selenium-containing amount of selection carries out Wheat rich in organic selenium screening varieties for the arable land of 0.18mg/kg
Experiment, the surrounding in the soil region to be tested set the protection row of width 1.1m and protection row and test soil it
Between stay and set 0.4m aisle wide, the wheat breed planted on protection row is Anhui Xi Maiemai 251-24.Trial zone and blank
The soil in area is deep ploughed, tilling depth 26cm, and the land equalization is divided into the first trial zone, the second trial zone and blank
Area, every mu to apply the standard of 1200kg, applies farm manure.With amount of application 120kg/ mus of standard to the first trial zone and the
, for the mineral fertilizer containing selenium of 200mg/kg carries out planting preceding intervention, tried in the first trial zone and second using selenium content two trial zones
Test area and mark off 24 areas respectively for 6.7m2Experimental cultivation area, mark off 24 areas for 6.7m in blank district2's
Blank growing area, the interval and the interval and adjacent experimental cultivation area of adjacent blank growing area in adjacent experimental cultivation area
With blank growing area at intervals of 0.5m.
It is randomly assigned to plant 24 wheats of kind in 24 experimental cultivation areas of the first trial zone, similarly in the second examination
Test area and blank test area and plant 24 above-mentioned wheats of kind at random；The application rate standard of wheat seed is broadcast for mu
14.5kg, and with the 0.2% of seed weight olefin conversion carry out sterilized seed dressing prior to seeding；The depth of wheat cultivation is 4cm, OK
Away from being 20cm.24 above-mentioned wheats of kind be respectively the western wheat 0638-1 in Anhui, red No. 3-2 of state, poplar wheat 23-3, China 2152-6,
Hubei Province wheat 18-7, Nan Nong 14Y106-8, letter wheat 8811-9, peaceful wheat 26-10, China 2566-11, raise wheat 20-12, Hubei Province wheat 580-13, Hubei Province
Wheat 23-15, assist wheat 25-16, China 1168-17, China 2668-18, assist wheat 55-19, Hubei Province grace No. 7-21, Zheng wheat 9023-23, E Mai
251-24, Hubei Province wheat 170-5, E-en No.6-14, Hubei Province wheat 596-20, assist wheat 35-22, Hubei Province wheat 352-4.
It is the organic selenium-rich liquid fertilizer of 30mg/L in the heading flowering period spraying concentration of wheat, wherein the richness that every mu of ground is sprayed
Se content in Organic Selenium liquid fertilizer is 1.4g；In the period of seedling establishment of wheat, mu applies the urea of 25kg；In the boot stage of wheat, mu
Apply the urea of 7kg.Before wheat jointing, sprayed only with wheat straw, weeds are removed；Prevent excessive growth and later stage from falling with paclobutrazol
Volt.Mid-June, gathered in, dried, and the wheat of the first trial zone and the second trial zone is carried out Se content detection and
And compare the per mu yield of the first trial zone, the second trial zone and each kind wheat of blank district.
To the product to be detected of the trial zone in embodiment 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5, embodiment 6
The testing result for planting the selenium content of wheat is shown in Table 1, table 2, table 3, table 4, the table 5, (unit of total selenium and organic selenium content of table 6：μg/
100g)。
The wheat selenium content of the trial zone of the embodiment 1 of table 1
| Kind | Total selenium | Organic Selenium | Organic Selenium accounting | Type |
| The western wheat 0638-1 in Anhui | 21.9 | 17.9 | 81.7% | Organic selenium-rich |
| Red No. 3-2 of state | 30 | 25.2 | 84.0% | Organic selenium-rich |
| Poplar wheat 23-3 | 23.7 | 21 | 88.6% | Organic selenium-rich |
| Magnificent 2152-6 | 39.4 | 33.3 | 84.5% | Organic selenium-rich |
| Hubei Province wheat 18-7 | 32.3 | 28.3 | 87.6% | Organic selenium-rich |
| Southern agriculture 14Y106-8 | 32.2 | 26.2 | 81.4% | Organic selenium-rich |
| Letter wheat 8811-9 | 32.5 | 26 | 80.0% | Organic selenium-rich |
| Peaceful wheat 26-10 | 24.5 | 19.9 | 81.2% | Organic selenium-rich |
| Magnificent 2566-11 | 20.4 | 18.3 | 89.7% | Organic selenium-rich |
| Raise wheat 20-12 | 21.6 | 18.7 | 86.6% | Organic selenium-rich |
| Hubei Province wheat 580-13 | 27.5 | 24.4 | 88.7% | Organic selenium-rich |
| Hubei Province wheat 23-15 | 33 | 28.4 | 86.1% | Organic selenium-rich |
| Assist wheat 25-16 | 39 | 33.8 | 86.7% | Organic selenium-rich |
| Magnificent 1168-17 | 28.9 | 24.4 | 84.4% | Organic selenium-rich |
| Magnificent 2668-18 | 30.2 | 26.1 | 86.4% | Organic selenium-rich |
| Assist wheat 55-19 | 26.7 | 23 | 86.1% | Organic selenium-rich |
| Hubei Province grace No. 7-21 | 31.7 | 26.3 | 83.0% | Organic selenium-rich |
| Zheng wheat 9023-23 | 22 | 18.7 | 85.0% | Organic selenium-rich |
| Hubei Province wheat 251-24 | 23.7 | 19.6 | 82.7% | Organic selenium-rich |
| Hubei Province wheat 170-5 | 16.9 | 14.4 | 85.2% | Containing selenium |
| E-en No.6-14 | 15.5 | 14.3 | 92.3% | Containing selenium |
| Hubei Province wheat 596-20 | 18.2 | 14.8 | 81.3% | Containing selenium |
| Assist wheat 35-22 | 16.1 | 13.8 | 85.7% | Containing selenium |
| Hubei Province wheat 352-4 | 14.7 | - | - | Containing selenium |
Note：The content of total selenium and Organic Selenium is the average content of the first trial zone and the second trial zone.
The wheat selenium content of the trial zone of the embodiment 2 of table 2
| Kind | Total selenium | Organic Selenium | Organic Selenium accounting | Type |
| The western wheat 0638-1 in Anhui | 22 | 18 | 81.8% | Organic selenium-rich |
| Red No. 3-2 of state | 31 | 26.2 | 84.5% | Organic selenium-rich |
| Poplar wheat 23-3 | 24.7 | 22 | 89.1% | Organic selenium-rich |
| Magnificent 2152-6 | 40.4 | 34.3 | 84.9% | Organic selenium-rich |
| Hubei Province wheat 18-7 | 33.3 | 29.3 | 88.0% | Organic selenium-rich |
| Southern agriculture 14Y106-8 | 33.2 | 27.2 | 81.9% | Organic selenium-rich |
| Letter wheat 8811-9 | 33.5 | 27 | 80.6% | Organic selenium-rich |
| Peaceful wheat 26-10 | 25.5 | 20.9 | 82.0% | Organic selenium-rich |
| Magnificent 2566-11 | 21.4 | 19.3 | 90.2% | Organic selenium-rich |
| Raise wheat 20-12 | 22.6 | 18.7 | 82.7% | Organic selenium-rich |
| Hubei Province wheat 580-13 | 28.5 | 25.4 | 89.1% | Organic selenium-rich |
| Hubei Province wheat 23-15 | 34 | 29.4 | 86.5% | Organic selenium-rich |
| Assist wheat 25-16 | 40 | 34.8 | 87.0% | Organic selenium-rich |
| Magnificent 1168-17 | 29.9 | 25.4 | 84.9% | Organic selenium-rich |
| Magnificent 2668-18 | 31.2 | 27.1 | 86.9% | Organic selenium-rich |
| Assist wheat 55-19 | 27.7 | 24 | 86.6% | Organic selenium-rich |
| Hubei Province grace No. 7-21 | 32.7 | 27.3 | 83.5% | Organic selenium-rich |
| Zheng wheat 9023-23 | 23 | 19.7 | 85.7% | Organic selenium-rich |
| Hubei Province wheat 251-24 | 24.7 | 20.6 | 83.4% | Organic selenium-rich |
| Hubei Province wheat 170-5 | 17.9 | 15.4 | 86.0% | Containing selenium |
The wheat selenium content of the trial zone of the embodiment 3 of table 3
Note：The content of total selenium and Organic Selenium is the average content of the first trial zone, the second trial zone and the 3rd trial zone.
The wheat selenium content of the trial zone of the embodiment 4 of table 4
| Kind | Total selenium | Organic Selenium | Organic Selenium accounting | Type |
| The western wheat 0638-1 in Anhui | 22.4 | 18.5 | 82.6% | Organic selenium-rich |
| Red No. 3-2 of state | 30.5 | 25.8 | 84.6% | Organic selenium-rich |
| Poplar wheat 23-3 | 24.2 | 21.6 | 89.3% | Organic selenium-rich |
| Magnificent 2152-6 | 39.9 | 33.9 | 85.0% | Organic selenium-rich |
| Hubei Province wheat 18-7 | 32.8 | 28.9 | 88.1% | Organic selenium-rich |
| Southern agriculture 14Y106-8 | 32.7 | 26.8 | 82.0% | Organic selenium-rich |
| Letter wheat 8811-9 | 33 | 26.6 | 80.6% | Organic selenium-rich |
| Peaceful wheat 26-10 | 25 | 20.5 | 82.0% | Organic selenium-rich |
| Magnificent 2566-11 | 20.9 | 18.9 | 90.4% | Organic selenium-rich |
| Raise wheat 20-12 | 22.1 | 19.3 | 87.3% | Organic selenium-rich |
| Hubei Province wheat 580-13 | 28 | 25 | 89.3% | Organic selenium-rich |
| Hubei Province wheat 23-15 | 33.7 | 28.8 | 85.5% | Organic selenium-rich |
| Assist wheat 25-16 | 39.7 | 34.2 | 86.1% | Organic selenium-rich |
| Magnificent 1168-17 | 29.6 | 24.8 | 83.8% | Organic selenium-rich |
| Magnificent 2668-18 | 30.9 | 26.5 | 85.8% | Organic selenium-rich |
| Assist wheat 55-19 | 27.4 | 23.4 | 85.4% | Organic selenium-rich |
| Hubei Province grace No. 7-21 | 32.4 | 26.7 | 82.4% | Organic selenium-rich |
| Zheng wheat 9023-23 | 22.7 | 19.1 | 84.1% | Organic selenium-rich |
| Hubei Province wheat 251-24 | 24.4 | 20 | 82.0% | Organic selenium-rich |
| Hubei Province wheat 170-5 | 17.6 | 14.8 | 84.1% | Containing selenium |
| E-en No.6-14 | 16.2 | 14.7 | 90.7% | Containing selenium |
| Hubei Province wheat 596-20 | 18.9 | 15.2 | 80.4% | Containing selenium |
| Assist wheat 35-22 | 16.8 | 14.2 | 84.5% | Containing selenium |
| Hubei Province wheat 352-4 | 15.4 | - | - | Containing selenium |
Note：The content of total selenium and Organic Selenium is the average content of the first trial zone and the second trial zone.
The wheat selenium content of the trial zone of the embodiment 5 of table 5
| Kind | Total selenium | Organic Selenium | Organic Selenium accounting | Type |
| The western wheat 0638-1 in Anhui | 21.5 | 17.7 | 82.3% | Organic selenium-rich |
| Red No. 3-2 of state | 30.5 | 25.9 | 84.9% | Organic selenium-rich |
| Poplar wheat 23-3 | 24.2 | 21.7 | 89.7% | Organic selenium-rich |
| Magnificent 2152-6 | 39.9 | 34 | 85.2% | Organic selenium-rich |
| Hubei Province wheat 18-7 | 32.8 | 29 | 88.4% | Organic selenium-rich |
| Southern agriculture 14Y106-8 | 32.7 | 26.9 | 82.3% | Organic selenium-rich |
| Letter wheat 8811-9 | 33 | 26.7 | 80.9% | Organic selenium-rich |
| Peaceful wheat 26-10 | 25 | 20.6 | 82.4% | Organic selenium-rich |
| Magnificent 2566-11 | 20.9 | 19 | 90.9% | Organic selenium-rich |
| Raise wheat 20-12 | 22.1 | 18.4 | 83.3% | Organic selenium-rich |
| Hubei Province wheat 580-13 | 28 | 25.1 | 89.6% | Organic selenium-rich |
| Hubei Province wheat 23-15 | 33.5 | 29.1 | 86.9% | Organic selenium-rich |
| Assist wheat 25-16 | 39.5 | 34.5 | 87.3% | Organic selenium-rich |
| Magnificent 1168-17 | 29.4 | 25.1 | 85.4% | Organic selenium-rich |
| Magnificent 2668-18 | 30.7 | 26.8 | 87.3% | Organic selenium-rich |
| Assist wheat 55-19 | 27.2 | 23.7 | 87.1% | Organic selenium-rich |
| Hubei Province grace No. 7-21 | 32.2 | 27 | 83.9% | Organic selenium-rich |
| Zheng wheat 9023-23 | 22.5 | 19.4 | 86.2% | Organic selenium-rich |
| Hubei Province wheat 251-24 | 24.2 | 20.3 | 83.9% | Organic selenium-rich |
| Hubei Province wheat 170-5 | 17.4 | 15.1 | 86.8% | Containing selenium |
The wheat selenium content of the trial zone of the embodiment 6 of table 6
Note：The content of total selenium and Organic Selenium is the average content of the first trial zone and the second trial zone.
Consolidated statement 1, table 2, table 3, table 4, table 5, the result of table 6 show, the western wheat 0638-1 in Anhui, red No. 3-2 of state, poplar wheat 23-
3rd, China 2152-6, Hubei Province wheat 18-7, Nan Nong 14Y106-8, letter wheat 8811-9, peaceful wheat 26-10, China 2566-11, raise wheat 20-12, Hubei Province
Wheat 580-13, Hubei Province wheat 23-15, assist wheat 25-16, China 1168-17, China 2668-18, assist wheat 55-19, Hubei Province grace No. 7-21, Zheng Mai
9023-23, Hubei Province wheat 251-24 are Wheat rich in organic selenium kind, and can be seen that the screening technique result stabilization that the present invention is provided,
It is reliable.
To trial zone and blank pair in embodiment 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5 and embodiment 6
Per mu yield according to the wheat of each kind in area is compared.Embodiment 1, embodiment 2, embodiment 3, embodiment 4, embodiment
5 and the blank district expression in the following table of embodiment 6 be respectively control group 1, control group 2, control group 3, control group 4, right
According to group 5, control group 6；The trial zone of embodiment 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5 and embodiment 6 is in following table
In expression be respectively test group 1, test group 2, test group 3, test group 4, test group 5, test group 6.The results are shown in Table 7 (per mu yields
Amount unit：kg).
Table 7 each per mu yield of kind wheat under different planting environments
The result of consolidated statement 7 shows, in the case of soil selenium-containing, red No. 3 of state, raise wheat No. 23, peaceful wheat 26, China 2566, Hubei Province
Wheat 580, the yield of wheat 25, Hubei Province grace 7, Hubei Province wheat 251, Hubei Province wheat 170, E-en No.6 and E Mai 596 is assisted to rise or protected substantially
Hold constant, illustrate that above-mentioned kind under conditions of soil selenium-containing, can not only lift the Se content of wheat, can also be in certain journey
The per mu yield of wheat is lifted on degree or keeps yield constant, further promote the economic benefit of wheat；And the yield drop of China 2668
It is low more than 30%, the production for being unfavorable for for carrying out Wheat rich in organic selenium from for the angle of production.
In sum, the screening technique of the Wheat rich in organic selenium kind of the embodiment of the present invention, can determine different wheats pair
The absorption accumulation ability of selenium element, so as to filter out the wheat breed of organic selenium-rich, beneficial to the product carried out when Se-rich wheat is planted
Kind of selection, it is to avoid the blindness of production, improves the economic benefit of production.
Embodiments described above is a part of embodiment of the invention, rather than whole embodiments.Reality of the invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of selected implementation of the invention
Example.Based on the embodiment in the present invention, what those of ordinary skill in the art were obtained under the premise of creative work is not made
Every other embodiment, belongs to the scope of protection of the invention.
Claims (10)
1. a kind of screening technique of Wheat rich in organic selenium kind, it is characterised in that including：
Experiment soil of the selection soil selenium-containing amount less than or equal to 0.2mg/kg, the experiment soil includes trial zone, in the examination
Test area and sow kind wheat to be detected, the trial zone carries out selenium-rich intervention, each of results is detected after results with Selenium-rich nutrient
Total selenium content and organic selenium content of the individual kind wheat to be detected, when the total selenium content of wheat that the trial zone is harvested is
0.2mg/kg-0.5mg/kg, and Organic Selenium content be more than or equal to 80% when, judges the kind wheat to be detected as be rich in
Machine selenium wheat breed.
2. the screening technique of Wheat rich in organic selenium kind according to claim 1, it is characterised in that the experiment Division
It is multiple experimental cultivation areas, each described kind wheat to be detected is planted in experimental cultivation area described at least one.
3. the screening technique of Wheat rich in organic selenium kind according to claim 2, it is characterised in that the trial zone includes
First trial zone and the second trial zone in the experimental cultivation area containing equal number, each described kind wheat to be detected point
Do not tried described at least one of experimental cultivation area described at least one of first trial zone and second trial zone
Test growing area plantation.
4. the screening technique of Wheat rich in organic selenium kind according to claim 2, it is characterised in that each described experiment kind
The area equation and area of growing area are 6.7m2-15m2。
5. the screening technique of Wheat rich in organic selenium kind according to claim 4, it is characterised in that the experiment soil is also
Including not using the Selenium-rich nutrient to carry out the blank district of the selenium-rich intervention, the blank district includes multiple
Blank growing area, the area of the blank growing area is equal to the area in the experimental cultivation area, by each kind to be detected
Wheat planting is in blank growing area described at least one, harvesting and the relatively same kind wheat to be detected is in the experiment
Growing area is planted and in the per mu yield of the resulting wheat of blank growing area plantation, when the same kind wheat to be detected
When the per mu yield in the experimental cultivation area in the per mu yield of the blank growing area than reducing 30%, then judge described to be detected
Kind wheat is not the Wheat rich in organic selenium kind.
6. the screening technique of Wheat rich in organic selenium kind according to claim 5, it is characterised in that the trial zone and institute
The area ratio for stating blank district is 2:1.
7. the screening technique of Wheat rich in organic selenium kind according to claim 5, it is characterised in that the selenium-rich intervention bag
Intervene after including the preceding intervention of plantation and plantation, it is in the trial zone in the kind wheat cultivation to be detected to intervene before the plantation
Before, the mineral fertilizer containing selenium is applied into the trial zone；Intervention is the heading in the kind wheat to be detected after the plantation
Blooming stage sprays in the blade face of the kind wheat to be detected organic selenium-rich liquid fertilizer, and the heading flowering period is Qi Suihou's
5d-7d。
8. the screening technique of Wheat rich in organic selenium kind according to claim 7, it is characterised in that the mineral containing selenium
The administration standard of fertilizer is 100kg/ mus -120kg/ mus, and the content of the selenium in the mineral fertilizer containing selenium is more than or equal to 200mg/kg.
9. the screening technique of Wheat rich in organic selenium kind according to claim 7, it is characterised in that the organic selenium-rich liquid
The application concentration of state fertilizer is 30mg/L, and the application concentration is containing in every liter of organic selenium-rich liquid fertilizer after dilute with water
Selenium amount is 30mg, while the selenium content of every mu of organic selenium-rich liquid fertilizer of ground administration is 1g-1.5g.
10. the screening technique of Wheat rich in organic selenium kind according to claim 9, it is characterised in that what every mu of ground was applied
The selenium content of the organic selenium-rich liquid fertilizer is 1.2g.
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