CN106666240A - Fermented feed for litopenaeus vannamei and production method thereof - Google Patents

Abstract

The invention discloses a fermented feed for litopenaeus vannamei and a production method thereof. The technical scheme of the fermented feed for the litopenaeus vannamei comprises the following ingredients in percentage by weight: 40-70% of skimmed rice bran, 2-2.5% of brown sugar, 2.5-5% of baker's yeast, 20-35% of an EM stock solution, and 5-10% of monobutyrin. The production method of the fermented feed for the litopenaeus vannamei comprises the following steps: (1), performing ultra-fine grinding on the skimmed rice bran by using a superfine grinding machine until the fineness reaches 80 mesh; (2), uniformly mixing the brown sugar, the bake' yeast, the EM stock solution and the monobutyrin with the skimmed rice bran powder prepared in the step (1) according to the percentage by weight; and (3), transferring the mixture prepared in the step (2) into a sealed anaerobic environment, and carrying out fermentation at 20-37 DEG C for 20-30 hours. The technical scheme provided by the the invention has the following advantages: the raw materials are easy to obtain, the preparation method is simple, and the cost is relatively low; moreover, the fermented feed can be directly used for feeding the litopenaeus vannamei, so that the usage method is simple and convenient. The fermented feed is capable of increasing food intake of the litopenaeus vannamei, as well as inhibiting vibrio breeding in the litopenaeus vannamei, so as to improve breeding success rate.

Description

A kind of Environment of Litopenaeus vannamei Low fermented feed and its production method

Technical field

The present invention relates to feed processing technology field, more particularly to a kind of Environment of Litopenaeus vannamei Low fermented feed and its producer Method.

Background technology

In the last few years, with the raising of prawn culturing density, the expansion of scale is cultivated, shrimp disease problem is also increasingly tight Weight；Especially because vibrio parahaemolytious causes prawn Deaths syndrome（EMS）Even more increasing is brought to shrimp culture industry Loss.For problem above, many researchers it is also proposed corresponding disease of prawn precautionary measures or enhancing prawn premunition Related art method.Due to easily producing drug resistance with traditional antibiotic even forbidden drug preventing and treating shrimp disease, formed Medicament residue, brings the Tough questions such as food safety hazard and environmental pollution.Therefore pass through nutrition regulation and exempt from from nutritional point The method that epidemic disease stimulates improves prawn autoimmunity activity, so that the death rate after reducing the generation of disease of prawn or falling ill is a kind of The method of environmental protection.Research and practice prove that it is to effectively improve cultivation to control cause of disease to grow by the regulation and control of internal and external environment The technological means of success rate, is the necessary guarantee for promoting shrimp culture industry sustainable health development.

The content of the invention

In view of the shortcomings of the prior art, can suppress to vannamei boone pair it is a primary object of the present invention to provide one kind The fermented feed that vibrios grows in shrimp body, the fermented feed can improve water quality while vibrios growth in prawn body is reduced, The grazing rate of prawn is improved simultaneously.

To achieve the above object, the invention provides following technical scheme：A kind of Environment of Litopenaeus vannamei Low fermented feed, with weight Meter, including following component：Defatted rice bran 40 ~ 70%, brown sugar 2 ~ 2.5%, Saccharomyces cerevisiae 2.5 ~ 5%, EM stostes 20 ~ 35%, only son's acid Glyceride 5 ~ 10%.

Including following component preferably, by weight,：Defatted rice bran 50%, brown sugar 5%, Saccharomyces cerevisiae 5%, EM stostes 30%, Only son's acid glyceride 10%.

A kind of production method of Environment of Litopenaeus vannamei Low fermented feed, including following production stage：

（1）, defatted rice bran first carried out into ultramicro grinding in micronizer reach 80 mesh fineness；

（2）, by brown sugar, Saccharomyces cerevisiae, EM stostes, only son's acid glyceride and step（1）Obtained defatted rice bran by weight hundred Divide than well mixed；

（3）, by step（2）Obtained mixture is transferred in the environment of sealing anaerobic, and fermentation 20 ~ 30 is small under the conditions of 20-37 DEG C When.

Preferably, step（3）In, fermentation time is 24 hours.

The present invention has the following advantages that the present invention can effectively improve prawn and be infected in breeding process relative to prior art The probability of vibrios disease, reduces the growth of vibrios in gut of shrimp, while improving prawn grazing rate, promotes prawn to ingest and give birth to It is long.This have the advantage that：Raw material is simple and easy to get, and compound method is simple, and cost is relatively low；Can directly feed and be taken the photograph to prawn Food, it is easy to use；Prawn food ration can be improved, while suppress vibrios in prawn body growing, cultivation success rate is improved.

Specific embodiment

A kind of Environment of Litopenaeus vannamei Low fermented feed, by weight, including following component：Defatted rice bran 40 ~ 70%, brown sugar 2 ~ 2.5%, Saccharomyces cerevisiae 2.5 ~ 5%, EM stostes 20 ~ 35%, only son's acid glyceride 5 ~ 10%.

The preferred plan of Environment of Litopenaeus vannamei Low fermented feed of the invention is, defatted rice bran 50%, brown sugar 5%, Saccharomyces cerevisiae 5%, EM stostes 30%, only son's acid glyceride 10%.

The production method of above-mentioned Environment of Litopenaeus vannamei Low fermented feed, including following production stage：

（1）, first defatted rice bran carried out in micronizer into ultramicro grinding reach 100% crossing 80 mesh fineness；

（2）, by brown sugar, Saccharomyces cerevisiae, EM stostes, only son's acid glyceride and step（1）Obtained defatted rice bran by weight hundred Divide than well mixed；

（3）, by step（2）Obtained mixture is transferred in the environment of sealing anaerobic, and fermentation 20 ~ 30 is small under the conditions of 20-37 DEG C When.

Further, step（3）In, fermentation time is 24 hours.

Embodiment 1

1st, feed prepares：

Extracting degreasing rice bran 50kg crosses 80 eye mesh screens through ultramicro grinding 100%, and then by itself and brown sugar 5kg, Saccharomyces cerevisiae 5kg, EM are former Liquid 30kg, only son's acid glyceride 10kg are well mixed, and are transferred in closed container, and ferment 24 hours, i.e. cost under the conditions of 25 DEG C Fermented feed.

2nd, culture experiment：

Culture experiment is carried out in the proving ground of Guangdong Evergreen Feed Industry Co., Ltd. 863.Experiment institute the aquarium that uses for The Glass fibre reinforced plastic tub of volume 300L.Cultivation seawater used is sand filter water, and each aquarium inflow is 0.5 L/min.During experiment Water temperature is controlled to 29.0 ± 0.5 °C, and salinity is 29 ‰, and dissolved oxygen maintains 7mg/l or so.The prawn of selection original body mass 0.25g It is randomly assigned in 6 aquariums, 2 test process groups, every group of 3 repetitions, each aquarium is put 30 tails experiment shrimp in a suitable place to breed, thrown daily Feed 2 times, control group normally feeds prawn perfect compound feed, and experimental group feeds 90% complete feed and 10% fermented feed. Experiment is carried out 8 weeks altogether.

3rd, sample collection and chemical analysis

After culture experiment terminates, every barrel of shrimp body gross weight is weighed respectively, record shrimp body number, calculate every barrel of average weight of shrimp.This Afterwards, 10 tail prawns are randomly selected for every barrel, determines enteron aisle vibrios content.All data use one-way analysis of variance (ANOVA), when Significant difference (P between treatment<0.05) when, being checked with Tukey ' s carries out Multiple range test analysis.All statistical analyses exist Carried out in the softwares of SPSS 11.0.

4 result of the tests

Feed group	Whole opisthosoma weight	Survival rate %	Enteron aisle vibrios content（Individual/ml）
				Control group	49.3	83.3b	2890a
Experimental group	49.2	95.6a	1480b
				Pooled S.E.M.a	3.2	4.3	325
ANOVAb
				F values	0.97	4.869	6.72
P values	0.403	0.001	0.002

Shown by above result of the test, the prawn fermented feed that the present invention is provided can significantly improve prawn culturing survival rate, dropped Vibrios content in low gut of shrimp.

Embodiment 2

According to defatted rice bran 70%, brown sugar 2.5%, Saccharomyces cerevisiae 2.5%, EM stostes 20%, the ratio mixing of only son's acid glyceride 5% Uniformly, then in the closed container under the conditions of 30 DEG C fermentation 24 hours fermented feed product.2 months-June in 2016 is in Jiang Sunan The logical such as Dong Guangrong villages Sang Zhi China pool mouthful uses.This area is that hut supports shrimp.Totally 30 huts, 0.8 mu of the area of each canopy, put seedling 30000/canopy.15 huts normally feed prawn perfect compound feed, and 15 huts are matched somebody with somebody using the prawn that fermented feed substitutes 15% in addition Feed is closed, whole process is used.After the sunrise shrimp of June 10,30 mouthfuls of yield and success rate in pond are calculated.Result shows, the 15 of control group Mouth pond, due to there is EMS in breeding process, causes have 4 mouthfuls of row pools not cultivate successfully, and 11 mouthfuls of pond average products are in addition 450 jin；And using 15 mouthfuls of ponds of fermented feed, cultivation all successes, average every 960 jin of the yield of hut.

Result of the test explanation can improve hut prawn culturing using fermented feed of the invention in production practices Success rate, and improve prawn culturing yield.

Embodiment 3

According to defatted rice bran 50%, brown sugar 5%, Saccharomyces cerevisiae 2.5%, EM stostes 32.5%, the ratio of only son's acid glyceride 10% is by thing Material is well mixed, and defatted rice bran is first crushed to 100% and crosses 80 mesh with micronizer.Then in the closed pile polybag of anaerobic Fermentation 24 hours, 26-32 DEG C of temperature, i.e. cost fermented feed.This batch produces totally 100 tons of fermented feed, and in May, 2016-September exists Used in Lianyungang of Jiangsu and Yancheng Region soil pool prawn culturing family, use ratio 10-30% substitutes corresponding mixed feed.10 Carry out market survey month, withdraw 33 parts of questionnaire, by statistical analysis, the raiser using the product is generally reflected, throw The prawn grazing rate for feeding the fermented feed is significantly improved, and the thicker nigrescence color of enteron aisle, cultivation survival rate with do not use the product phase Than significantly improving, and feeding for mixed feed is saved, reduced corresponding feed coefficient.

The above is only the preferred embodiment of the present invention, and protection scope of the present invention is not limited merely to above-mentioned implementation Example, all technical schemes belonged under thinking of the present invention belong to protection scope of the present invention.It should be pointed out that for the art Those of ordinary skill for, some improvements and modifications without departing from the principles of the present invention, these improvements and modifications Should be regarded as protection scope of the present invention.

Claims (4)

1. a kind of Environment of Litopenaeus vannamei Low fermented feed, it is characterised in that by weight, including following component：Defatted rice bran 40 ~ 70%, Brown sugar 2 ~ 2.5%, Saccharomyces cerevisiae 2.5 ~ 5%, EM stostes 20 ~ 35%, only son's acid glyceride 5 ~ 10%.

2. a kind of Environment of Litopenaeus vannamei Low fermented feed according to claim 1, it is characterised in that by weight, including it is as follows Component：Defatted rice bran 50%, brown sugar 5%, Saccharomyces cerevisiae 5%, EM stostes 30%, only son's acid glyceride 10%.

3. a kind of production method of Environment of Litopenaeus vannamei Low fermented feed, it is characterised in that including following production stage：

（1）, defatted rice bran first carried out into ultramicro grinding in micronizer reach 80 mesh fineness；

（2）, by brown sugar, Saccharomyces cerevisiae, EM stostes, only son's acid glyceride and step（1）Obtained defatted rice bran by weight hundred Divide than well mixed；

（3）, by step（2）Obtained mixture is transferred in the environment of sealing anaerobic, and fermentation 20 ~ 30 is small under the conditions of 20-37 DEG C When.

4. a kind of production method of Environment of Litopenaeus vannamei Low fermented feed according to claim 3, it is characterised in that step（3） In, fermentation time is 24 hours.