CN106589004A - Treating method for pig manure - Google Patents
Treating method for pig manure Download PDFInfo
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- CN106589004A CN106589004A CN201610891084.1A CN201610891084A CN106589004A CN 106589004 A CN106589004 A CN 106589004A CN 201610891084 A CN201610891084 A CN 201610891084A CN 106589004 A CN106589004 A CN 106589004A
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- swine excrement
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07G—COMPOUNDS OF UNKNOWN CONSTITUTION
- C07G15/00—Hormones
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J1/00—Normal steroids containing carbon, hydrogen, halogen or oxygen, not substituted in position 17 beta by a carbon atom, e.g. estrane, androstane
- C07J1/0003—Androstane derivatives
- C07J1/0018—Androstane derivatives substituted in position 17 beta, not substituted in position 17 alfa
- C07J1/0022—Androstane derivatives substituted in position 17 beta, not substituted in position 17 alfa the substituent being an OH group free esterified or etherified
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J1/00—Normal steroids containing carbon, hydrogen, halogen or oxygen, not substituted in position 17 beta by a carbon atom, e.g. estrane, androstane
- C07J1/0051—Estrane derivatives
- C07J1/0066—Estrane derivatives substituted in position 17 beta not substituted in position 17 alfa
- C07J1/007—Estrane derivatives substituted in position 17 beta not substituted in position 17 alfa the substituent being an OH group free esterified or etherified
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Endocrinology (AREA)
- Fodder In General (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a treating method for pig manure. The method comprises the following steps: (1) weighing fresh pig manure from a certain livestock farm, adding methanol, carrying out stirring and dilution, then carrying out ultrasonic extraction and filtering with an ultrafiltration membrane so as to obtain filter residues and a filtrate, subjecting the filtrate to dissolving and shaking with petroleum ether, extraction with ethyl acetate twice and purification with a gel column, then carrying out elution by using a mixture of absolute ethyl alcohol and chloroform in a ratio of 2: 9, carrying out drying so as to obtain a precipitate and then subjecting the precipitate to hormone detection; (2) adding water into the filter residues obtained in the step (1) for dilution, carrying out stirring, putting the diluted filter residues into a fermentation tank, adding inoculant for a microbial fermentation reaction, then adding a RW ferment agent, continuing the reaction, adding water for dilution after completion of the reaction and subjecting prepared mixed liquid to standing for subsequent usage; and (3) introducing the mixed liquid diluted in the step (2) into a membrane bioreactor for a nitratlon reaction so as to remove nitrogen and phosphorus substances in the pig manure, subjecting treated liquid to filtering via a nanometer modified solid-liquid separation membrane so as to obtain manure having undergone impurity removal, drying the manure and then recycling the dried manure.
Description
Technical field
The present invention relates to swine excrement processing technology field, particularly relates to a kind of processing method of swine excrement.
Background technology
The recycling of swine excrement is to environmental conservation and develops the economy and has certain meaning, hormone present in swine excrement
Class material can detect and react that the survival condition of animal includes reproduction procreation information etc., and this point is especially in the research of wild animal
In have a very big significance, can obtain on the premise of animal is not alarmed detection material, and hormone equally to high-volume cultivate
Zooscopy its health there is good meaning.Swine excrement is good natural fertilizers, but swine excrement is not said in representative
Can be used directly without process, the material such as hormone, nitrogen, phosphorus for containing in swine excrement is comprehended to soil, lake ripple, surface water without place
Etc. cause certain pollution, therefore swine excrement should carry out certain process before recycling to it, so as to reduce harmful substance
Harm to environment.
Recycling of the China to swine excrement is mostly production biological organic fertilizer, or carries out ecologic breeding etc., pig manure
Just many to be prepared with fermentation method, to the Processing Technology Research of swine excrement harmful substance not enough, the harmful substance in swine excrement is not
Jing process, life-time service can have a great impact to environment, and the place of harmful substance is carried out before how recycling to swine excrement
Reason, and realize that the depth optimization of swine excrement is exactly our problems to be studied.
The content of the invention
Present invention solves the technical problem that being just to provide a kind of processing method of swine excrement.
The technical scheme is that:
A kind of processing method of swine excrement, comprises the following steps:
(1) certain plant's animal fresh excreta, plus the methanol stirring that the mass concentration of quintuple water, triplication is 90% are taken
After dilution, place at 6 DEG C and be placed in again in supersonic extractors after 15h, with ethanol, acetone, the ethyl acetate of 15 times of amounts with 5:1:2
Ratio is mixed at extractant, 15-20 DEG C carries out supersound extraction 15min, and filtering residue and filtrate, gained filter are obtained after ultrafiltration membrance filter
Liquid adjusts pH to be shaken with the petroleum ether dissolution of equimultiple under conditions of 3-6, and upper solution, recovered under reduced pressure are discarded after color separated
Be extracted with ethyl acetate again after organic solvent twice, cross after DEAE fibre columns again with polydextran gel and thin base polydextran gel with
1:The gel column purification of 1 ratio mixing, afterwards with dehydrated alcohol and chloroform with 2:9 ratio mixing carries out eluting, dried
After be precipitated thing, carry out hormone test;
(2) filtering residue plus 5 times of amount water dilutions to obtaining in (1) is stirred, and is placed in fermentation vat and is added microbial inoculum to carry out microorganism
Ferment reacts, and adds RW enzyme agents to continue to react 5h in 1% ratio after reaction 8h, and reaction adds again the water dilution of 8 times of amounts after terminating,
Stir, it is standby;
(3) by the mixed liquor after dilution in (2) with 14-20m3The speed of/s is passed through in membrane bioreactor to be carried out nitrifying instead
Should, the nitrogen phosphorus substance contained in feces is removed, gained treatment fluid Jing nano modification solid-liquid separation membrane filtrations obtain the excrement after remove impurity
Just, drying and processing is carried out to it, then is recycled.
Preferably, the ethanol mass concentration in described (1) in extractant is 85%.
Preferably, the polydextran gel and thin base polydextran gel are needed at normal temperatures and pressures, are 60- with mass concentration
70% alcohol solution dipping 15h, being stirred continuously expands gel, is washed away to be filtered dry after residual ethanol with salt-free water and reuses.
Preferably, the drying meanss are vacuum drying, and products therefrom is uniform.
Preferably, the microbial inoculum is bacillus cereuss, yeast and Lactobacillus buchneri with 7:2:3 ratio is mixed, institute
Fermentation temperature is stated for 30-45 DEG C, pH regulator scope is 5.3-6.7.
Preferably, the membrane bioreactor is integral type, is capable of achieving the cross-flow reaction effect of face, and floor space is little,
More fully, the film is a kind of new utilization process for suspension polymerization, and adds metallic zinc and obtained ceramic membrane for reaction.
The invention has the beneficial effects as follows:A kind of processing method of swine excrement of the present invention, not only can be in swine excrement
Zoohormone is extracted, and on the one hand can have certain understanding to animal bodies situation by the hormone for extracting, and on the other hand may be used
The hormone in swine excrement is removed, for subsequent recovery process condition is provided, and by biofermentation and membrane bioreactor, eliminated
Nitrogen phosphorus in swine excrement, and make swine excrement obtain depth optimization, be conducive to environmental protection.
Specific embodiment
Embodiment 1:
A kind of processing method of swine excrement, comprises the following steps:
(1) certain plant's animal fresh excreta, plus the methanol stirring that the mass concentration of quintuple water, triplication is 90% are taken
After dilution, place at 6 DEG C and be placed in again in supersonic extractors after 15h, with ethanol, acetone, the ethyl acetate of 15 times of amounts with 5:1:2
Ratio is mixed into extractant, and supersound extraction 15min is carried out at 15 DEG C, and filtering residue and filtrate, gained filtrate are obtained after ultrafiltration membrance filter
Adjust pH to be shaken with the petroleum ether dissolution of equimultiple under conditions of 3, upper solution is discarded after color separated, recovered under reduced pressure is organic
It is extracted with ethyl acetate again after solvent twice, crosses after DEAE fibre columns again with polydextran gel and thin base polydextran gel with 1:1
The gel column purification of ratio mixing, afterwards with dehydrated alcohol and chloroform with 2:9 ratio mixing carries out eluting, after dried
To precipitate, hormone test is carried out;
(2) filtering residue plus 5 times of amount water dilutions to obtaining in (1) is stirred, and is placed in fermentation vat and is added microbial inoculum to carry out microorganism
Ferment reacts, and adds RW enzyme agents to continue to react 5h in 1% ratio after reaction 8h, and reaction adds again the water dilution of 8 times of amounts after terminating,
Stir, it is standby;
(3) by the mixed liquor after dilution in (2) with 14m3The speed of/s is passed through in membrane bioreactor carries out nitration reaction,
The nitrogen phosphorus substance contained in feces is removed, gained treatment fluid Jing nano modification solid-liquid separation membrane filtrations obtain the feces after remove impurity,
Drying and processing is carried out to it, then is recycled.
Wherein, the ethanol mass concentration in described (1) in extractant is 85%;The polydextran gel and thin base glucosan
Gel is needed at normal temperatures and pressures, and with the alcohol solution dipping 15h that mass concentration is 60%, being stirred continuously expands gel, with nothing
Saline is washed away to be filtered dry after residual ethanol and reused;The drying meanss are vacuum drying, and products therefrom is uniform;The microbial inoculum is bud
Spore bacillus, yeast and Lactobacillus buchneri are with 7:2:3 ratio is mixed, and the fermentation temperature is 30 DEG C, pH regulator scope
For 5.3;The membrane bioreactor is integral type, is capable of achieving the cross-flow reaction effect of face, and floor space is little, and reaction is more filled
Point, the film is a kind of new utilization process for suspension polymerization, and adds metallic zinc and obtained ceramic membrane.
Embodiment 2:
A kind of processing method of swine excrement, comprises the following steps:
(1) certain plant's animal fresh excreta, plus the methanol stirring that the mass concentration of quintuple water, triplication is 90% are taken
After dilution, place at 6 DEG C and be placed in again in supersonic extractors after 15h, with ethanol, acetone, the ethyl acetate of 15 times of amounts with 5:1:2
Ratio is mixed into extractant, and supersound extraction 15min is carried out at 17.5 DEG C, and filtering residue and filtrate, gained filter are obtained after ultrafiltration membrance filter
Liquid adjusts pH to be shaken with the petroleum ether dissolution of equimultiple under conditions of 4.5, and upper solution, recovered under reduced pressure are discarded after color separated
Be extracted with ethyl acetate again after organic solvent twice, cross after DEAE fibre columns again with polydextran gel and thin base polydextran gel with
1:The gel column purification of 1 ratio mixing, afterwards with dehydrated alcohol and chloroform with 2:9 ratio mixing carries out eluting, dried
After be precipitated thing, carry out hormone test;
(2) filtering residue plus 5 times of amount water dilutions to obtaining in (1) is stirred, and is placed in fermentation vat and is added microbial inoculum to carry out microorganism
Ferment reacts, and adds RW enzyme agents to continue to react 5h in 1% ratio after reaction 8h, and reaction adds again the water dilution of 8 times of amounts after terminating,
Stir, it is standby;
(3) by the mixed liquor after dilution in (2) with 17m3The speed of/s is passed through in membrane bioreactor carries out nitration reaction,
The nitrogen phosphorus substance contained in feces is removed, gained treatment fluid Jing nano modification solid-liquid separation membrane filtrations obtain the feces after remove impurity,
Drying and processing is carried out to it, then is recycled.
Wherein, the ethanol mass concentration in described (1) in extractant is 85%;The polydextran gel and thin base glucosan
Gel is needed at normal temperatures and pressures, and with the alcohol solution dipping 15h that mass concentration is 65%, being stirred continuously expands gel, with nothing
Saline is washed away to be filtered dry after residual ethanol and reused;The drying meanss are vacuum drying, and products therefrom is uniform;The microbial inoculum is bud
Spore bacillus, yeast and Lactobacillus buchneri are with 7:2:3 ratio is mixed, and the fermentation temperature is 37.5 DEG C, pH regulator model
Enclose for 6;The membrane bioreactor is integral type, is capable of achieving the cross-flow reaction effect of face, and floor space is little, and reaction is more filled
Point, the film is a kind of new utilization process for suspension polymerization, and adds metallic zinc and obtained ceramic membrane.
Embodiment 3:
A kind of processing method of swine excrement, comprises the following steps:
(1) certain plant's animal fresh excreta, plus the methanol stirring that the mass concentration of quintuple water, triplication is 90% are taken
After dilution, place at 6 DEG C and be placed in again in supersonic extractors after 15h, with ethanol, acetone, the ethyl acetate of 15 times of amounts with 5:1:2
Ratio is mixed into extractant, and supersound extraction 15min is carried out at 20 DEG C, and filtering residue and filtrate, gained filtrate are obtained after ultrafiltration membrance filter
Adjust pH to be shaken with the petroleum ether dissolution of equimultiple under conditions of 6, upper solution is discarded after color separated, recovered under reduced pressure is organic
It is extracted with ethyl acetate again after solvent twice, crosses after DEAE fibre columns again with polydextran gel and thin base polydextran gel with 1:1
The gel column purification of ratio mixing, afterwards with dehydrated alcohol and chloroform with 2:9 ratio mixing carries out eluting, after dried
To precipitate, hormone test is carried out;
(2) filtering residue plus 5 times of amount water dilutions to obtaining in (1) is stirred, and is placed in fermentation vat and is added microbial inoculum to carry out microorganism
Ferment reacts, and adds RW enzyme agents to continue to react 5h in 1% ratio after reaction 8h, and reaction adds again the water dilution of 8 times of amounts after terminating,
Stir, it is standby;
(3) by the mixed liquor after dilution in (2) with 20m3The speed of/s is passed through in membrane bioreactor carries out nitration reaction,
The nitrogen phosphorus substance contained in feces is removed, gained treatment fluid Jing nano modification solid-liquid separation membrane filtrations obtain the feces after remove impurity,
Drying and processing is carried out to it, then is recycled.
Wherein, the ethanol mass concentration in described (1) in extractant is 85%;The polydextran gel and thin base glucosan
Gel is needed at normal temperatures and pressures, and with the alcohol solution dipping 15h that mass concentration is 70%, being stirred continuously expands gel, with nothing
Saline is washed away to be filtered dry after residual ethanol and reused;The drying meanss are vacuum drying, and products therefrom is uniform;The microbial inoculum is bud
Spore bacillus, yeast and Lactobacillus buchneri are with 7:2:3 ratio is mixed, and the fermentation temperature is 45 DEG C, pH regulator scope
For 6.7;The membrane bioreactor is integral type, is capable of achieving the cross-flow reaction effect of face, and floor space is little, and reaction is more filled
Point, the film is a kind of new utilization process for suspension polymerization, and adds metallic zinc and obtained ceramic membrane.
Analysis of experimental data:
Extraction research of the inventive method to hormone in swine excrement:
Test method:Fresh swine excrement is taken, with the method for the present invention hormone in feces is extracted, detected with euzymelinked immunosorbent assay (ELISA)
The response rate of estradiol and testosterone illustrates that the present invention can effectively extract and reclaim swashing in swine excrement up to 80% or so in feces
Element.
Research of the inventive method to nitrogen and phosphorus content in swine excrement:
Feces after above-mentioned hormone is extracted are passed through in membrane bioreactor after being fermented, and reaction detects feces after terminating
In nitrogen and phosphorus content, clearance illustrates that the present invention can effectively remove feces harmful substance up to 80%, and the feces Jing after processing enter
Row is further recycled, and pollution will not be brought to environment.
Finally it should be noted that:Above example only to illustrate technical scheme, rather than a limitation;Although
The present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those within the art that:It still may be used
To modify to the technical scheme described in previous embodiment, or equivalent is carried out to which part technical characteristic;And
These modifications are replaced, and do not make the spirit and model of the essence disengaging embodiment of the present invention technical scheme of appropriate technical solution
Enclose.
Claims (6)
1. a kind of processing method of swine excrement, it is characterised in that the method comprising the steps of:
(1) certain plant's animal fresh excreta, plus the methanol agitation and dilution that the mass concentration of quintuple water, triplication is 90% are taken
Afterwards, place at 6 DEG C and be placed in again in supersonic extractors after 15h, with ethanol, acetone, the ethyl acetate of 15 times of amounts with 5:1:2 ratio
Being mixed at extractant, 15-20 DEG C carries out supersound extraction 15min, and filtering residue and filtrate are obtained after ultrafiltration membrance filter, and gained filtrate is adjusted
PH is shaken under conditions of 3-6 with the petroleum ether dissolution of equimultiple, and upper solution is discarded after color separated, and recovered under reduced pressure is organic
It is extracted with ethyl acetate again after solvent twice, crosses after DEAE fibre columns again with polydextran gel and thin base polydextran gel with 1:1
The gel column purification of ratio mixing, afterwards with dehydrated alcohol and chloroform with 2:9 ratio mixing carries out eluting, after dried
To precipitate, hormone test is carried out;
(2) to the filtering residue that obtains in (1) plus 5 times of amount water dilution stirrings, being placed in fermentation vat, to add microbial inoculum to carry out fermentable anti-
Should, add RW enzyme agents to continue to react 5h in 1% ratio after reaction 8h, reaction adds again the water dilution of 8 times of amounts, stirring after terminating
Uniformly, it is standby;
(3) mixed liquor after dilution in (2) is passed through in membrane bioreactor with the speed of 14-20m3/s carries out nitration reaction,
The nitrogen phosphorus substance contained in feces is removed, gained treatment fluid Jing nano modification solid-liquid separation membrane filtrations obtain the feces after remove impurity,
Drying and processing is carried out to it, then is recycled.
2. the processing method of a kind of swine excrement according to claim 1, it is characterised in that in (1) in extractant
Ethanol mass concentration is 85%.
3. the processing method of a kind of swine excrement according to claim 1, it is characterised in that the polydextran gel and thin base
Polydextran gel is needed at normal temperatures and pressures, and with the alcohol solution dipping 15h that mass concentration is 60-70%, being stirred continuously makes gel
Expansion, is washed away to be filtered dry after residual ethanol with salt-free water and reuses.
4. the processing method of a kind of swine excrement according to claim 1, it is characterised in that the drying meanss are dry for vacuum
It is dry.
5. the processing method of a kind of swine excrement according to claim 2, it is characterised in that the microbial inoculum be bacillus cereuss,
Yeast and Lactobacillus buchneri are with 7:2:3 ratio is mixed, and the fermentation temperature is 30-45 DEG C, and pH regulator scope is
5.3-6.7。
6. the processing method of a kind of swine excrement according to claim 1, it is characterised in that the membrane bioreactor is
Body formula, the film is a kind of new utilization process for suspension polymerization, and adds metallic zinc and obtained ceramic membrane.
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CN201610891084.1A CN106589004A (en) | 2016-10-12 | 2016-10-12 | Treating method for pig manure |
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CN201610891084.1A CN106589004A (en) | 2016-10-12 | 2016-10-12 | Treating method for pig manure |
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Citations (5)
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CN101307332A (en) * | 2008-06-23 | 2008-11-19 | 昆明东燃科技开发有限公司 | Innocent comprehensive approach method for human feces by biotechnology |
CN102735768A (en) * | 2012-06-06 | 2012-10-17 | 北京师范大学 | Process for jointly detecting estrogens and their associations in livestock and poultry excrements |
CN104671615A (en) * | 2015-03-04 | 2015-06-03 | 江苏龙腾工程设计有限公司 | Process for treating livestock and poultry breeding sewage entering Lake Taihu |
CN105174617A (en) * | 2015-09-11 | 2015-12-23 | 武汉市宜能环保科技有限公司 | Wastewater advanced treatment system of large-scale pig farm |
CN105198160A (en) * | 2015-09-25 | 2015-12-30 | 重庆淏园环保科技有限公司 | Method for treating domestic sewage by aid of complex bacteria |
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2016
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CN101307332A (en) * | 2008-06-23 | 2008-11-19 | 昆明东燃科技开发有限公司 | Innocent comprehensive approach method for human feces by biotechnology |
CN102735768A (en) * | 2012-06-06 | 2012-10-17 | 北京师范大学 | Process for jointly detecting estrogens and their associations in livestock and poultry excrements |
CN104671615A (en) * | 2015-03-04 | 2015-06-03 | 江苏龙腾工程设计有限公司 | Process for treating livestock and poultry breeding sewage entering Lake Taihu |
CN105174617A (en) * | 2015-09-11 | 2015-12-23 | 武汉市宜能环保科技有限公司 | Wastewater advanced treatment system of large-scale pig farm |
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