CN106589004A - Treating method for pig manure - Google Patents

Treating method for pig manure Download PDF

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Publication number
CN106589004A
CN106589004A CN201610891084.1A CN201610891084A CN106589004A CN 106589004 A CN106589004 A CN 106589004A CN 201610891084 A CN201610891084 A CN 201610891084A CN 106589004 A CN106589004 A CN 106589004A
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CN
China
Prior art keywords
reaction
dilution
swine excrement
processing method
carrying
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CN201610891084.1A
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Chinese (zh)
Inventor
杜建伟
项赟
张明杨
温勇
陈雷
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South China Institute of Environmental Science of Ministry of Ecology and Environment
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South China Institute of Environmental Science of Ministry of Ecology and Environment
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Priority to CN201610891084.1A priority Critical patent/CN106589004A/en
Publication of CN106589004A publication Critical patent/CN106589004A/en
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07GCOMPOUNDS OF UNKNOWN CONSTITUTION
    • C07G15/00Hormones
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07JSTEROIDS
    • C07J1/00Normal steroids containing carbon, hydrogen, halogen or oxygen, not substituted in position 17 beta by a carbon atom, e.g. estrane, androstane
    • C07J1/0003Androstane derivatives
    • C07J1/0018Androstane derivatives substituted in position 17 beta, not substituted in position 17 alfa
    • C07J1/0022Androstane derivatives substituted in position 17 beta, not substituted in position 17 alfa the substituent being an OH group free esterified or etherified
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07JSTEROIDS
    • C07J1/00Normal steroids containing carbon, hydrogen, halogen or oxygen, not substituted in position 17 beta by a carbon atom, e.g. estrane, androstane
    • C07J1/0051Estrane derivatives
    • C07J1/0066Estrane derivatives substituted in position 17 beta not substituted in position 17 alfa
    • C07J1/007Estrane derivatives substituted in position 17 beta not substituted in position 17 alfa the substituent being an OH group free esterified or etherified
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/30Fuel from waste, e.g. synthetic alcohol or diesel
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Endocrinology (AREA)
  • Fodder In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention discloses a treating method for pig manure. The method comprises the following steps: (1) weighing fresh pig manure from a certain livestock farm, adding methanol, carrying out stirring and dilution, then carrying out ultrasonic extraction and filtering with an ultrafiltration membrane so as to obtain filter residues and a filtrate, subjecting the filtrate to dissolving and shaking with petroleum ether, extraction with ethyl acetate twice and purification with a gel column, then carrying out elution by using a mixture of absolute ethyl alcohol and chloroform in a ratio of 2: 9, carrying out drying so as to obtain a precipitate and then subjecting the precipitate to hormone detection; (2) adding water into the filter residues obtained in the step (1) for dilution, carrying out stirring, putting the diluted filter residues into a fermentation tank, adding inoculant for a microbial fermentation reaction, then adding a RW ferment agent, continuing the reaction, adding water for dilution after completion of the reaction and subjecting prepared mixed liquid to standing for subsequent usage; and (3) introducing the mixed liquid diluted in the step (2) into a membrane bioreactor for a nitratlon reaction so as to remove nitrogen and phosphorus substances in the pig manure, subjecting treated liquid to filtering via a nanometer modified solid-liquid separation membrane so as to obtain manure having undergone impurity removal, drying the manure and then recycling the dried manure.

Description

A kind of processing method of swine excrement
Technical field
The present invention relates to swine excrement processing technology field, particularly relates to a kind of processing method of swine excrement.
Background technology
The recycling of swine excrement is to environmental conservation and develops the economy and has certain meaning, hormone present in swine excrement Class material can detect and react that the survival condition of animal includes reproduction procreation information etc., and this point is especially in the research of wild animal In have a very big significance, can obtain on the premise of animal is not alarmed detection material, and hormone equally to high-volume cultivate Zooscopy its health there is good meaning.Swine excrement is good natural fertilizers, but swine excrement is not said in representative Can be used directly without process, the material such as hormone, nitrogen, phosphorus for containing in swine excrement is comprehended to soil, lake ripple, surface water without place Etc. cause certain pollution, therefore swine excrement should carry out certain process before recycling to it, so as to reduce harmful substance Harm to environment.
Recycling of the China to swine excrement is mostly production biological organic fertilizer, or carries out ecologic breeding etc., pig manure Just many to be prepared with fermentation method, to the Processing Technology Research of swine excrement harmful substance not enough, the harmful substance in swine excrement is not Jing process, life-time service can have a great impact to environment, and the place of harmful substance is carried out before how recycling to swine excrement Reason, and realize that the depth optimization of swine excrement is exactly our problems to be studied.
The content of the invention
Present invention solves the technical problem that being just to provide a kind of processing method of swine excrement.
The technical scheme is that:
A kind of processing method of swine excrement, comprises the following steps:
(1) certain plant's animal fresh excreta, plus the methanol stirring that the mass concentration of quintuple water, triplication is 90% are taken After dilution, place at 6 DEG C and be placed in again in supersonic extractors after 15h, with ethanol, acetone, the ethyl acetate of 15 times of amounts with 5:1:2 Ratio is mixed at extractant, 15-20 DEG C carries out supersound extraction 15min, and filtering residue and filtrate, gained filter are obtained after ultrafiltration membrance filter Liquid adjusts pH to be shaken with the petroleum ether dissolution of equimultiple under conditions of 3-6, and upper solution, recovered under reduced pressure are discarded after color separated Be extracted with ethyl acetate again after organic solvent twice, cross after DEAE fibre columns again with polydextran gel and thin base polydextran gel with 1:The gel column purification of 1 ratio mixing, afterwards with dehydrated alcohol and chloroform with 2:9 ratio mixing carries out eluting, dried After be precipitated thing, carry out hormone test;
(2) filtering residue plus 5 times of amount water dilutions to obtaining in (1) is stirred, and is placed in fermentation vat and is added microbial inoculum to carry out microorganism Ferment reacts, and adds RW enzyme agents to continue to react 5h in 1% ratio after reaction 8h, and reaction adds again the water dilution of 8 times of amounts after terminating, Stir, it is standby;
(3) by the mixed liquor after dilution in (2) with 14-20m3The speed of/s is passed through in membrane bioreactor to be carried out nitrifying instead Should, the nitrogen phosphorus substance contained in feces is removed, gained treatment fluid Jing nano modification solid-liquid separation membrane filtrations obtain the excrement after remove impurity Just, drying and processing is carried out to it, then is recycled.
Preferably, the ethanol mass concentration in described (1) in extractant is 85%.
Preferably, the polydextran gel and thin base polydextran gel are needed at normal temperatures and pressures, are 60- with mass concentration 70% alcohol solution dipping 15h, being stirred continuously expands gel, is washed away to be filtered dry after residual ethanol with salt-free water and reuses.
Preferably, the drying meanss are vacuum drying, and products therefrom is uniform.
Preferably, the microbial inoculum is bacillus cereuss, yeast and Lactobacillus buchneri with 7:2:3 ratio is mixed, institute Fermentation temperature is stated for 30-45 DEG C, pH regulator scope is 5.3-6.7.
Preferably, the membrane bioreactor is integral type, is capable of achieving the cross-flow reaction effect of face, and floor space is little, More fully, the film is a kind of new utilization process for suspension polymerization, and adds metallic zinc and obtained ceramic membrane for reaction.
The invention has the beneficial effects as follows:A kind of processing method of swine excrement of the present invention, not only can be in swine excrement Zoohormone is extracted, and on the one hand can have certain understanding to animal bodies situation by the hormone for extracting, and on the other hand may be used The hormone in swine excrement is removed, for subsequent recovery process condition is provided, and by biofermentation and membrane bioreactor, eliminated Nitrogen phosphorus in swine excrement, and make swine excrement obtain depth optimization, be conducive to environmental protection.
Specific embodiment
Embodiment 1:
A kind of processing method of swine excrement, comprises the following steps:
(1) certain plant's animal fresh excreta, plus the methanol stirring that the mass concentration of quintuple water, triplication is 90% are taken After dilution, place at 6 DEG C and be placed in again in supersonic extractors after 15h, with ethanol, acetone, the ethyl acetate of 15 times of amounts with 5:1:2 Ratio is mixed into extractant, and supersound extraction 15min is carried out at 15 DEG C, and filtering residue and filtrate, gained filtrate are obtained after ultrafiltration membrance filter Adjust pH to be shaken with the petroleum ether dissolution of equimultiple under conditions of 3, upper solution is discarded after color separated, recovered under reduced pressure is organic It is extracted with ethyl acetate again after solvent twice, crosses after DEAE fibre columns again with polydextran gel and thin base polydextran gel with 1:1 The gel column purification of ratio mixing, afterwards with dehydrated alcohol and chloroform with 2:9 ratio mixing carries out eluting, after dried To precipitate, hormone test is carried out;
(2) filtering residue plus 5 times of amount water dilutions to obtaining in (1) is stirred, and is placed in fermentation vat and is added microbial inoculum to carry out microorganism Ferment reacts, and adds RW enzyme agents to continue to react 5h in 1% ratio after reaction 8h, and reaction adds again the water dilution of 8 times of amounts after terminating, Stir, it is standby;
(3) by the mixed liquor after dilution in (2) with 14m3The speed of/s is passed through in membrane bioreactor carries out nitration reaction, The nitrogen phosphorus substance contained in feces is removed, gained treatment fluid Jing nano modification solid-liquid separation membrane filtrations obtain the feces after remove impurity, Drying and processing is carried out to it, then is recycled.
Wherein, the ethanol mass concentration in described (1) in extractant is 85%;The polydextran gel and thin base glucosan Gel is needed at normal temperatures and pressures, and with the alcohol solution dipping 15h that mass concentration is 60%, being stirred continuously expands gel, with nothing Saline is washed away to be filtered dry after residual ethanol and reused;The drying meanss are vacuum drying, and products therefrom is uniform;The microbial inoculum is bud Spore bacillus, yeast and Lactobacillus buchneri are with 7:2:3 ratio is mixed, and the fermentation temperature is 30 DEG C, pH regulator scope For 5.3;The membrane bioreactor is integral type, is capable of achieving the cross-flow reaction effect of face, and floor space is little, and reaction is more filled Point, the film is a kind of new utilization process for suspension polymerization, and adds metallic zinc and obtained ceramic membrane.
Embodiment 2:
A kind of processing method of swine excrement, comprises the following steps:
(1) certain plant's animal fresh excreta, plus the methanol stirring that the mass concentration of quintuple water, triplication is 90% are taken After dilution, place at 6 DEG C and be placed in again in supersonic extractors after 15h, with ethanol, acetone, the ethyl acetate of 15 times of amounts with 5:1:2 Ratio is mixed into extractant, and supersound extraction 15min is carried out at 17.5 DEG C, and filtering residue and filtrate, gained filter are obtained after ultrafiltration membrance filter Liquid adjusts pH to be shaken with the petroleum ether dissolution of equimultiple under conditions of 4.5, and upper solution, recovered under reduced pressure are discarded after color separated Be extracted with ethyl acetate again after organic solvent twice, cross after DEAE fibre columns again with polydextran gel and thin base polydextran gel with 1:The gel column purification of 1 ratio mixing, afterwards with dehydrated alcohol and chloroform with 2:9 ratio mixing carries out eluting, dried After be precipitated thing, carry out hormone test;
(2) filtering residue plus 5 times of amount water dilutions to obtaining in (1) is stirred, and is placed in fermentation vat and is added microbial inoculum to carry out microorganism Ferment reacts, and adds RW enzyme agents to continue to react 5h in 1% ratio after reaction 8h, and reaction adds again the water dilution of 8 times of amounts after terminating, Stir, it is standby;
(3) by the mixed liquor after dilution in (2) with 17m3The speed of/s is passed through in membrane bioreactor carries out nitration reaction, The nitrogen phosphorus substance contained in feces is removed, gained treatment fluid Jing nano modification solid-liquid separation membrane filtrations obtain the feces after remove impurity, Drying and processing is carried out to it, then is recycled.
Wherein, the ethanol mass concentration in described (1) in extractant is 85%;The polydextran gel and thin base glucosan Gel is needed at normal temperatures and pressures, and with the alcohol solution dipping 15h that mass concentration is 65%, being stirred continuously expands gel, with nothing Saline is washed away to be filtered dry after residual ethanol and reused;The drying meanss are vacuum drying, and products therefrom is uniform;The microbial inoculum is bud Spore bacillus, yeast and Lactobacillus buchneri are with 7:2:3 ratio is mixed, and the fermentation temperature is 37.5 DEG C, pH regulator model Enclose for 6;The membrane bioreactor is integral type, is capable of achieving the cross-flow reaction effect of face, and floor space is little, and reaction is more filled Point, the film is a kind of new utilization process for suspension polymerization, and adds metallic zinc and obtained ceramic membrane.
Embodiment 3:
A kind of processing method of swine excrement, comprises the following steps:
(1) certain plant's animal fresh excreta, plus the methanol stirring that the mass concentration of quintuple water, triplication is 90% are taken After dilution, place at 6 DEG C and be placed in again in supersonic extractors after 15h, with ethanol, acetone, the ethyl acetate of 15 times of amounts with 5:1:2 Ratio is mixed into extractant, and supersound extraction 15min is carried out at 20 DEG C, and filtering residue and filtrate, gained filtrate are obtained after ultrafiltration membrance filter Adjust pH to be shaken with the petroleum ether dissolution of equimultiple under conditions of 6, upper solution is discarded after color separated, recovered under reduced pressure is organic It is extracted with ethyl acetate again after solvent twice, crosses after DEAE fibre columns again with polydextran gel and thin base polydextran gel with 1:1 The gel column purification of ratio mixing, afterwards with dehydrated alcohol and chloroform with 2:9 ratio mixing carries out eluting, after dried To precipitate, hormone test is carried out;
(2) filtering residue plus 5 times of amount water dilutions to obtaining in (1) is stirred, and is placed in fermentation vat and is added microbial inoculum to carry out microorganism Ferment reacts, and adds RW enzyme agents to continue to react 5h in 1% ratio after reaction 8h, and reaction adds again the water dilution of 8 times of amounts after terminating, Stir, it is standby;
(3) by the mixed liquor after dilution in (2) with 20m3The speed of/s is passed through in membrane bioreactor carries out nitration reaction, The nitrogen phosphorus substance contained in feces is removed, gained treatment fluid Jing nano modification solid-liquid separation membrane filtrations obtain the feces after remove impurity, Drying and processing is carried out to it, then is recycled.
Wherein, the ethanol mass concentration in described (1) in extractant is 85%;The polydextran gel and thin base glucosan Gel is needed at normal temperatures and pressures, and with the alcohol solution dipping 15h that mass concentration is 70%, being stirred continuously expands gel, with nothing Saline is washed away to be filtered dry after residual ethanol and reused;The drying meanss are vacuum drying, and products therefrom is uniform;The microbial inoculum is bud Spore bacillus, yeast and Lactobacillus buchneri are with 7:2:3 ratio is mixed, and the fermentation temperature is 45 DEG C, pH regulator scope For 6.7;The membrane bioreactor is integral type, is capable of achieving the cross-flow reaction effect of face, and floor space is little, and reaction is more filled Point, the film is a kind of new utilization process for suspension polymerization, and adds metallic zinc and obtained ceramic membrane.
Analysis of experimental data:
Extraction research of the inventive method to hormone in swine excrement:
Test method:Fresh swine excrement is taken, with the method for the present invention hormone in feces is extracted, detected with euzymelinked immunosorbent assay (ELISA) The response rate of estradiol and testosterone illustrates that the present invention can effectively extract and reclaim swashing in swine excrement up to 80% or so in feces Element.
Research of the inventive method to nitrogen and phosphorus content in swine excrement:
Feces after above-mentioned hormone is extracted are passed through in membrane bioreactor after being fermented, and reaction detects feces after terminating In nitrogen and phosphorus content, clearance illustrates that the present invention can effectively remove feces harmful substance up to 80%, and the feces Jing after processing enter Row is further recycled, and pollution will not be brought to environment.
Finally it should be noted that:Above example only to illustrate technical scheme, rather than a limitation;Although The present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those within the art that:It still may be used To modify to the technical scheme described in previous embodiment, or equivalent is carried out to which part technical characteristic;And These modifications are replaced, and do not make the spirit and model of the essence disengaging embodiment of the present invention technical scheme of appropriate technical solution Enclose.

Claims (6)

1. a kind of processing method of swine excrement, it is characterised in that the method comprising the steps of:
(1) certain plant's animal fresh excreta, plus the methanol agitation and dilution that the mass concentration of quintuple water, triplication is 90% are taken Afterwards, place at 6 DEG C and be placed in again in supersonic extractors after 15h, with ethanol, acetone, the ethyl acetate of 15 times of amounts with 5:1:2 ratio Being mixed at extractant, 15-20 DEG C carries out supersound extraction 15min, and filtering residue and filtrate are obtained after ultrafiltration membrance filter, and gained filtrate is adjusted PH is shaken under conditions of 3-6 with the petroleum ether dissolution of equimultiple, and upper solution is discarded after color separated, and recovered under reduced pressure is organic It is extracted with ethyl acetate again after solvent twice, crosses after DEAE fibre columns again with polydextran gel and thin base polydextran gel with 1:1 The gel column purification of ratio mixing, afterwards with dehydrated alcohol and chloroform with 2:9 ratio mixing carries out eluting, after dried To precipitate, hormone test is carried out;
(2) to the filtering residue that obtains in (1) plus 5 times of amount water dilution stirrings, being placed in fermentation vat, to add microbial inoculum to carry out fermentable anti- Should, add RW enzyme agents to continue to react 5h in 1% ratio after reaction 8h, reaction adds again the water dilution of 8 times of amounts, stirring after terminating Uniformly, it is standby;
(3) mixed liquor after dilution in (2) is passed through in membrane bioreactor with the speed of 14-20m3/s carries out nitration reaction, The nitrogen phosphorus substance contained in feces is removed, gained treatment fluid Jing nano modification solid-liquid separation membrane filtrations obtain the feces after remove impurity, Drying and processing is carried out to it, then is recycled.
2. the processing method of a kind of swine excrement according to claim 1, it is characterised in that in (1) in extractant Ethanol mass concentration is 85%.
3. the processing method of a kind of swine excrement according to claim 1, it is characterised in that the polydextran gel and thin base Polydextran gel is needed at normal temperatures and pressures, and with the alcohol solution dipping 15h that mass concentration is 60-70%, being stirred continuously makes gel Expansion, is washed away to be filtered dry after residual ethanol with salt-free water and reuses.
4. the processing method of a kind of swine excrement according to claim 1, it is characterised in that the drying meanss are dry for vacuum It is dry.
5. the processing method of a kind of swine excrement according to claim 2, it is characterised in that the microbial inoculum be bacillus cereuss, Yeast and Lactobacillus buchneri are with 7:2:3 ratio is mixed, and the fermentation temperature is 30-45 DEG C, and pH regulator scope is 5.3-6.7。
6. the processing method of a kind of swine excrement according to claim 1, it is characterised in that the membrane bioreactor is Body formula, the film is a kind of new utilization process for suspension polymerization, and adds metallic zinc and obtained ceramic membrane.
CN201610891084.1A 2016-10-12 2016-10-12 Treating method for pig manure Pending CN106589004A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101307332A (en) * 2008-06-23 2008-11-19 昆明东燃科技开发有限公司 Innocent comprehensive approach method for human feces by biotechnology
CN102735768A (en) * 2012-06-06 2012-10-17 北京师范大学 Process for jointly detecting estrogens and their associations in livestock and poultry excrements
CN104671615A (en) * 2015-03-04 2015-06-03 江苏龙腾工程设计有限公司 Process for treating livestock and poultry breeding sewage entering Lake Taihu
CN105174617A (en) * 2015-09-11 2015-12-23 武汉市宜能环保科技有限公司 Wastewater advanced treatment system of large-scale pig farm
CN105198160A (en) * 2015-09-25 2015-12-30 重庆淏园环保科技有限公司 Method for treating domestic sewage by aid of complex bacteria

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101307332A (en) * 2008-06-23 2008-11-19 昆明东燃科技开发有限公司 Innocent comprehensive approach method for human feces by biotechnology
CN102735768A (en) * 2012-06-06 2012-10-17 北京师范大学 Process for jointly detecting estrogens and their associations in livestock and poultry excrements
CN104671615A (en) * 2015-03-04 2015-06-03 江苏龙腾工程设计有限公司 Process for treating livestock and poultry breeding sewage entering Lake Taihu
CN105174617A (en) * 2015-09-11 2015-12-23 武汉市宜能环保科技有限公司 Wastewater advanced treatment system of large-scale pig farm
CN105198160A (en) * 2015-09-25 2015-12-30 重庆淏园环保科技有限公司 Method for treating domestic sewage by aid of complex bacteria

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